reference standards

Summary

Summary: A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.

Top Publications

  1. pmc Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    Jo Vandesompele
    Center for Medical Genetics, Ghent University Hospital 1K5, De Pintelaan 185, B 9000 Ghent, Belgium
    Genome Biol 3:RESEARCH0034. 2002
  2. ncbi Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets
    Claus Lindbjerg Andersen
    Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital, Skejby, DK 8200 Aarhus N, Denmark
    Cancer Res 64:5245-50. 2004
  3. pmc A new mathematical model for relative quantification in real-time RT-PCR
    M W Pfaffl
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Germany
    Nucleic Acids Res 29:e45. 2001
  4. pmc Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
    Michael W Pfaffl
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Germany
    Nucleic Acids Res 30:e36. 2002
  5. pmc Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis
    Tomasz Czechowski
    Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany
    Plant Physiol 139:5-17. 2005
  6. ncbi Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress
    Nathalie Nicot
    Centre de Recherche Public Gabriel Lippmann, Environment and Biotechnologies Research Unit, 41 rue du Brill, L 4422 Belvaux, Luxembourg
    J Exp Bot 56:2907-14. 2005
  7. pmc Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR
    Ruibo Hu
    Institute of Crop Science, National Key Facility of Crop Gene Resource and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China
    BMC Mol Biol 10:93. 2009
  8. pmc The development of QUADAS: a tool for the quality assessment of studies of diagnostic accuracy included in systematic reviews
    Penny Whiting
    Centre for Reviews and Dissemination, University of York, England, UK
    BMC Med Res Methodol 3:25. 2003
  9. ncbi Real-time RT-PCR normalisation; strategies and considerations
    J Huggett
    Centre for Infectious Diseases and International Health, University College London, London, UK
    Genes Immun 6:279-84. 2005
  10. ncbi Quantitative mass spectrometric multiple reaction monitoring assays for major plasma proteins
    Leigh Anderson
    The Plasma Proteome Institute, Washington, DC 20009 3450, USA
    Mol Cell Proteomics 5:573-88. 2006

Detail Information

Publications355 found, 100 shown here

  1. pmc Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    Jo Vandesompele
    Center for Medical Genetics, Ghent University Hospital 1K5, De Pintelaan 185, B 9000 Ghent, Belgium
    Genome Biol 3:RESEARCH0034. 2002
    ....
  2. ncbi Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets
    Claus Lindbjerg Andersen
    Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital, Skejby, DK 8200 Aarhus N, Denmark
    Cancer Res 64:5245-50. 2004
    ..The presented strategy can be applied to evaluate the suitability of any normalization gene candidate in any kind of experimental design and should allow more reliable normalization of RT-PCR data...
  3. pmc A new mathematical model for relative quantification in real-time RT-PCR
    M W Pfaffl
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Germany
    Nucleic Acids Res 29:e45. 2001
    ..High accuracy and reproducibility (<2.5% variation) were reached in LightCycler PCR using the established mathematical model...
  4. pmc Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
    Michael W Pfaffl
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Germany
    Nucleic Acids Res 30:e36. 2002
    ..The latest software version of REST and examples for the correct use can be downloaded at http://www.wzw.tum.de/gene-quantification/...
  5. pmc Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis
    Tomasz Czechowski
    Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany
    Plant Physiol 139:5-17. 2005
    ..The developed PCR primers or hybridization probes for the novel reference genes will enable better normalization and quantification of transcript levels in Arabidopsis in the future...
  6. ncbi Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress
    Nathalie Nicot
    Centre de Recherche Public Gabriel Lippmann, Environment and Biotechnologies Research Unit, 41 rue du Brill, L 4422 Belvaux, Luxembourg
    J Exp Bot 56:2907-14. 2005
    ..The relative quantification of the hsp20.2 gene varied according to the internal control and the number of internal controls used, thus highlighting the importance of the choice of internal controls in such experiments...
  7. pmc Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR
    Ruibo Hu
    Institute of Crop Science, National Key Facility of Crop Gene Resource and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China
    BMC Mol Biol 10:93. 2009
    ..Thus, a systematic validation of reference genes is required to ensure proper normalization. For soybean, only a small number of validated reference genes are available to date...
  8. pmc The development of QUADAS: a tool for the quality assessment of studies of diagnostic accuracy included in systematic reviews
    Penny Whiting
    Centre for Reviews and Dissemination, University of York, England, UK
    BMC Med Res Methodol 3:25. 2003
    ..The aim of this project was to combine empirical evidence and expert opinion in a formal consensus method to develop a tool to be used in systematic reviews to assess the quality of primary studies of diagnostic accuracy...
  9. ncbi Real-time RT-PCR normalisation; strategies and considerations
    J Huggett
    Centre for Infectious Diseases and International Health, University College London, London, UK
    Genes Immun 6:279-84. 2005
    ..In this review we discuss the relative merits of different normalisation strategies and suggest a method of validation that will enable the measurement of biologically meaningful results...
  10. ncbi Quantitative mass spectrometric multiple reaction monitoring assays for major plasma proteins
    Leigh Anderson
    The Plasma Proteome Institute, Washington, DC 20009 3450, USA
    Mol Cell Proteomics 5:573-88. 2006
    ....
  11. pmc Identification and validation of reference genes for quantitative RT-PCR normalization in wheat
    Anna R Paolacci
    Dipartimento di Agrobiologia ed Agrochimica, Universita della Tuscia, Via S Camillo de Lellis, 01100 Viterbo, Italy
    BMC Mol Biol 10:11. 2009
    ..For selecting candidate reference genes we have developed a simple in silico method based on the data publicly available in the wheat databases Unigene and TIGR...
  12. pmc Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR
    Valeria Valente
    Department of Surgery and Anatomy, Faculty of Medicine, University of Sao Paulo, Av, dos Bandeirantes 3900, 140490 900, Ribeirao Preto, SP, Brazil
    BMC Mol Biol 10:17. 2009
    ..For glioblastoma, the most common type of tumor in the central nervous system, there was no previous report regarding this issue...
  13. pmc Exploring valid reference genes for gene expression studies in Brachypodium distachyon by real-time PCR
    Shin Young Hong
    Department of Chemistry, Seoul National University, Seoul, 151 742, Korea
    BMC Plant Biol 8:112. 2008
    ..Therefore, stable reference genes are necessary to normalize the gene expression data...
  14. pmc RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization
    Tomas Hruz
    Department of Biology, ETH Zurich, 8092 Zurich, Switzerland
    BMC Genomics 12:156. 2011
    ..There is increasing evidence, however, that the expression of commonly used reference genes can vary significantly in certain contexts...
  15. doi Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references
    Stéphanie Guénin
    Molecular Biology Centre, Centre de Ressources Régionales en Biologie Moléculaire, Universite de Picardie Jules Verne, Faculte des Sciences, 33 rue St Leu, F 80039 Amiens, France
    J Exp Bot 60:487-93. 2009
    ....
  16. pmc Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process
    Marino Expósito-Rodríguez
    Instituto de Productos Naturales y Agrobiología CSIC, Avda, Astrofísico Francisco Sánchez 3, 38206 La Laguna, Tenerife, Canary Islands, Spain
    BMC Plant Biol 8:131. 2008
    ..In the present report we describe the first systematic evaluation of potential internal controls during tomato development process to identify which are the most reliable for transcript quantification by real-time RT-PCR...
  17. pmc Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data
    Sinara Artico
    Department of Genetics, Federal University of Rio de Janeiro UFRJ, Rio de Janeiro, RJ, Brasil
    BMC Plant Biol 10:49. 2010
    ..Only a few studies on the identification of housekeeping gene have been carried on plants. Therefore qPCR studies on important crops such as cotton has been hampered by the lack of suitable reference genes...
  18. ncbi The STARD statement for reporting studies of diagnostic accuracy: explanation and elaboration
    Patrick M Bossuyt
    Department of Clinical Epidemiology and Biostatistics, Academic Medical Center University of Amsterdam, 1100 DE Amsterdam, The Netherlands
    Clin Chem 49:7-18. 2003
    ..Complete and informative reporting can only lead to better decisions in healthcare...
  19. pmc Identification of suitable endogenous control genes for microRNA gene expression analysis in human breast cancer
    Pamela A Davoren
    Department of Surgery, National University of Ireland, Galway, Ireland
    BMC Mol Biol 9:76. 2008
    ..This is the first study to identify reliable ECs for analysis of miRNA by RQ-PCR in human breast tissue...
  20. pmc Defining the true sensitivity of culture for the diagnosis of melioidosis using Bayesian latent class models
    Direk Limmathurotsakul
    Department of Tropical Hygiene, Mahidol University, Bangkok, Thailand
    PLoS ONE 5:e12485. 2010
    ....
  21. pmc Reference genes for accurate transcript normalization in citrus genotypes under different experimental conditions
    Valéria Mafra
    Laboratorio de Biotecnologia, Centro de Citricultura Sylvio Moreira, Cordeirópolis São Paulo, Brazil
    PLoS ONE 7:e31263. 2012
    ..This work constitutes the first systematic analysis for the selection of superior reference genes for transcript normalization in different citrus organs and under biotic stress...
  22. pmc Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae
    Marie Ange Teste
    CNRS, UMR5504, F 31400 Toulouse, France
    BMC Mol Biol 10:99. 2009
    ..The aim of this work was to search for a set of reference genes for reliable gene expression analysis in Saccharomyces cerevisiae...
  23. doi Markers of bone turnover for the prediction of fracture risk and monitoring of osteoporosis treatment: a need for international reference standards
    S Vasikaran
    Department of Core Clinical Pathology and Biochemistry, PathWest Laboratory Medicine, Royal Perth Hospital, Perth, WA, Australia
    Osteoporos Int 22:391-420. 2011
    ....
  24. pmc Mathematics of quantitative kinetic PCR and the application of standard curves
    R G Rutledge
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P E P S, PO Box 3800, Sainte Foy, Quebec G1V 4C7, Canada
    Nucleic Acids Res 31:e93. 2003
    ..A simplified method for absolute quantification is also proposed, in which quantitative scale is determined by DNA mass at threshold...
  25. doi Validation of reference genes for RT-qPCR studies of gene expression in banana fruit under different experimental conditions
    Lei Chen
    Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou, 510642, People s Republic of China
    Planta 234:377-90. 2011
    ..Taken together, our results provide guidelines for reference gene(s) selection under different experimental conditions and a foundation for more accurate and widespread use of RT-qPCR in banana...
  26. doi Selection of appropriate reference genes for gene expression studies by quantitative real-time polymerase chain reaction in cucumber
    Hongjian Wan
    State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People s Republic of China
    Anal Biochem 399:257-61. 2010
    ..This study also provides guidelines for selecting different reference genes under different conditions...
  27. doi Variations in clinical PET/CT operations: results of an international survey of active PET/CT users
    Thomas Beyer
    Department of Nuclear Medicine, University Hospital Essen, Essen, Germany
    J Nucl Med 52:303-10. 2011
    ..This study gathered information about clinical PET/CT operations worldwide to help guide discussions on the use and standardization of clinical PET/CT...
  28. doi Need for standardization of 18F-FDG PET/CT for treatment response assessments
    Ronald Boellaard
    Department of Nuclear Medicine and PET Research, VU University Medical Center, Amsterdam, The Netherlands
    J Nucl Med 52:93S-100S. 2011
    ..This article provides an overview of the need for standardization in relation to the specific use of SUVs and SUV changes in studies of treatment response assessments...
  29. ncbi A method for the absolute quantification of cDNA using real-time PCR
    Joseph A Whelan
    Onyvax Ltd, St George s Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK
    J Immunol Methods 278:261-9. 2003
    ..PicoGreen and reference standards are used to measure the amount of cDNA present in a sample using fluorescence...
  30. doi Identification of reference genes for reverse transcription quantitative real-time PCR normalization in pepper (Capsicum annuum L.)
    Hongjian Wan
    Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, People s Republic of China
    Biochem Biophys Res Commun 416:24-30. 2011
    ..Validation of these candidate genes could provide useful guidelines for reference gene selection in qRT-PCR studies in pepper...
  31. doi The 6-min walk distance in healthy subjects: reference standards from seven countries
    C Casanova
    Pulmonary Dept, Universidad La Candelaria, Santa Cruz de Tenerife, Spain
    Eur Respir J 37:150-6. 2011
    ..001). Age-specific reference standards of 6MWD were constructed for male and female adults...
  32. pmc Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation
    Yee Hwa Yang
    Department of Statistics, Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720 3860, USA
    Nucleic Acids Res 30:e15. 2002
    ..Lastly, to allow for comparisons of expression levels across slides, a robust method based on maximum likelihood estimation is proposed to adjust for scale differences among slides...
  33. pmc Cerulean, Venus, and VenusY67C FRET reference standards
    Srinagesh V Koushik
    Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD, USA
    Biophys J 91:L99-L101. 2006
    ..Since the results from each method were in good agreement, the FRET efficiency value of each construct could be determined with high accuracy and precision, thereby justifying their use as standards...
  34. pmc Model-based analysis of oligonucleotide arrays: expression index computation and outlier detection
    C Li
    Departments of Statistics and Human Genetics, University of California, Los Angeles, CA 90095
    Proc Natl Acad Sci U S A 98:31-6. 2001
    ..We also present model-based methods for identifying and handling cross-hybridizing probes and contaminating array regions. Applications of these results will be presented elsewhere...
  35. doi How to do successful gene expression analysis using real-time PCR
    Stefaan Derveaux
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Methods 50:227-30. 2010
    ....
  36. pmc Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR
    Asad Maroufi
    Department of Plant Production, Faculty of Bioscience Engineering, Coupure Links 653, Gent University, 9000 Ghent, Belgium
    BMC Mol Biol 11:15. 2010
    ..Thus far, only actin-2 has been used as a reference gene for qRT-PCR in chicory, and a full comparison of several candidate reference genes in chicory has not yet been reported...
  37. ncbi Housekeeping gene variability in normal and cancerous colorectal, pancreatic, esophageal, gastric and hepatic tissues
    Claudia Rubie
    Department of General, Vascular and Paediatric Surgery, University of the Saarland, Chirurgische Klinik, Gebaude 57, Homburg Saar 66421, Germany
    Mol Cell Probes 19:101-9. 2005
    ..In summary, our results can be used as guidance for other scientists studying various carcinomas for tissue-specific selection of the optimal housekeeping gene (HKG) to be used in normalizing target gene expression...
  38. pmc Importance of suitable reference gene selection for quantitative real-time PCR: special reference to mouse myocardial infarction studies
    Bert R Everaert
    Laboratory of Cell Biology and Histology, University of Antwerp, Antwerp, Belgium
    PLoS ONE 6:e23793. 2011
    ..In this study, we investigated the expression stability of 10 commonly used reference genes in a mouse myocardial infarction model...
  39. pmc Validation of endogenous reference genes for qRT-PCR analysis of human visceral adipose samples
    Rohini Mehta
    Molecular and Microbiology Department and Center for the Study of Genomics in Liver Diseases, George Mason University, Fairfax, VA, USA
    BMC Mol Biol 11:39. 2010
    ..In this study we validated candidate reference genes suitable for qRT-PCR profiling experiments using visceral adipose samples from obese and lean individuals...
  40. ncbi Identification of endogenous reference genes for qRT-PCR analysis in normal matched breast tumor tissues
    Bala Gur-Dedeoglu
    Department of Molecular Biology and Genetics, Faculty of Science, Bilkent University, Ankara, Turkey
    Oncol Res 17:353-65. 2009
    ....
  41. pmc mzML--a community standard for mass spectrometry data
    Lennart Martens
    Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
    Mol Cell Proteomics 10:R110.000133. 2011
    ..The resulting standard data format, mzML, is a well tested open-source format for mass spectrometer output files that can be readily utilized by the community and easily adapted for incremental advances in mass spectrometry technology...
  42. doi Evaluation of suitable reference gene for real-time PCR in human umbilical cord mesenchymal stem cells with long-term in vitro expansion
    Youwei Wang
    National Engineering Research Center of Cell Products, AmCellGene Co Ltd, TEDA, Tianjin 300457, People s Republic of China
    In Vitro Cell Dev Biol Anim 46:595-9. 2010
    ..Ywhaz and Rpl13a could be used as reference genes for relative gene quantification and normalization purposes in real-time PCR studies of human umbilical cord mesenchymal stem cells...
  43. pmc Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida
    Izaskun Mallona
    Genetics, Instituto de Biotecnología Vegetal, Universidad Politecnica de Cartagena, Cartagena, Spain
    BMC Plant Biol 10:4. 2010
    ..Ideally expression of these genes should be independent of the morphogenetic process or environmental condition tested as well as the methods used for RNA purification and analysis...
  44. pmc Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions
    Carla F Barsalobres-Cavallari
    Laboratório de Biotecnologia e Genética Molecular, Departamento de Genetica, Instituto de Biociencias, UNESP, Sao Paulo, Brazil
    BMC Mol Biol 10:1. 2009
    ..We also validated the expression profiles of the selected reference genes at various stages of development and under a specific biotic stress...
  45. doi Selection of suitable reference genes for real-time PCR studies of Atlantic halibut development
    Jorge M O Fernandes
    Department of Fisheries and Natural Sciences, Bodø Regional University, N 8049 Bodø, Norway
    Comp Biochem Physiol B Biochem Mol Biol 150:23-32. 2008
    ..We believe that either of these normalization factors can be used for future developmental gene expression studies in Atlantic halibut...
  46. ncbi Beta-actin is not a reliable loading control in Western blot analysis
    Angela Dittmer
    Klinik fur Gynakologie, Universitat Halle, Halle, Germany
    Electrophoresis 27:2844-5. 2006
    ..This shows that beta-Actin is not a reliable loading control in Western blot analysis. In general, it appeared that, at longer incubation times, antibodies seem to be less able to pick up differences in the level of its target protein...
  47. pmc NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins
    Kim D Pruitt
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Rm 6An 12J, 45 Center Drive, Bethesda, MD 20892 6510, USA
    Nucleic Acids Res 35:D61-5. 2007
    ..The format of all RefSeq records is validated, and an increasing number of tests are being applied to evaluate the quality of sequence and annotation, especially in the context of complete genomic sequence...
  48. ncbi Development of a new standard reference material: SRM 1955 (homocysteine and folate in human serum)
    Mary B Satterfield
    Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Anal Bioanal Chem 385:612-22. 2006
    ..Use of the new SRM should improve clinical measurements and will permit traceability to internationally recognized certified reference materials, as described by European Directive 98/79/EC on in vitro diagnostic medical devices...
  49. pmc Transcriptome sequencing of the Microarray Quality Control (MAQC) RNA reference samples using next generation sequencing
    Shrinivasrao P Mane
    Department of Biological Sciences, Virginia Tech, Blacksburg, VA 24061, USA
    BMC Genomics 10:264. 2009
    ..As a preliminary evaluation of these promising technologies, we performed deep sequencing of cDNA synthesized from the Microarray Quality Control (MAQC) reference RNA samples using Roche's 454 Genome Sequencer FLX...
  50. doi Reference genes for normalization: a study of rat brain tissue
    Birgit Egeskov Bonefeld
    Centre for Psychiatric Research, Aarhus University Hospital, Risskov, DK 8240 Risskov, Denmark
    Synapse 62:302-9. 2008
    ..These results underline the importance of choosing a proper normalization strategy when analyzing gene expression with qPCR. The strategy should be unbiased and carried out in every specific experimental setting...
  51. pmc Reference gene selection for quantitative real-time PCR normalization in Quercus suber
    Liliana Marum
    Instituto de Biologia Experimental e Tecnológica Instituto de Tecnologia Química e Biológica Universidade Nova de Lisboa, Oeiras, Portugal
    PLoS ONE 7:e35113. 2012
    ....
  52. doi Evaluation and identification of reliable reference genes for pharmacogenomics, toxicogenomics, and small RNA expression analysis
    Dongliang Chen
    Department of Biology, East Carolina University, Greenville, North Carolina 27858, USA
    J Cell Physiol 226:2469-77. 2011
    ..To widely use these genes as reference genes, these results should be corroborated by studies with other human cell lines and additional drugs classes and hormonal treatments...
  53. ncbi Selection of appropriate control genes to assess expression of tumor antigens using real-time RT-PCR
    Joeri L Aerts
    National Institutes of Health, Bethesda, MD, USA
    Biotechniques 36:84-6, 88, 90-1. 2004
    ....
  54. doi A review of solutions for diagnostic accuracy studies with an imperfect or missing reference standard
    Johannes B Reitsma
    Department of Clinical Epidemiology, Biostatistics and Bioinformatics, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    J Clin Epidemiol 62:797-806. 2009
    ..In diagnostic accuracy studies, the reference standard may be imperfect or not available in all patients. We systematically reviewed the proposed solutions for these situations and generated methodological guidance...
  55. doi Identification and validation of reference genes for normalization of transcripts from virus-infected Arabidopsis thaliana
    S T Lilly
    The New Zealand Institute for Plant and Food Research, Limited
    Mol Plant Microbe Interact 24:294-304. 2011
    ....
  56. doi Evaluation of endogenous control genes for gene expression studies across multiple tissues and in the specific sets of fat- and muscle-type samples of the pig
    Y R Gu
    Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya an, Sichuan, China
    J Anim Breed Genet 128:319-25. 2011
    ..Our report will serve as a valuable reference for other studies aimed at measuring tissue-specific mRNA abundance in porcine samples...
  57. doi Validation of reference genes for real-time quantitative PCR studies in gene expression levels of Lactobacillus casei Zhang
    Wenjing Zhao
    Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, School of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot, China
    J Ind Microbiol Biotechnol 38:1279-86. 2011
    ..The systematic validation of candidate reference genes is important for obtaining reliable analysis results of real-time quantitative PCR studies in gene expression levels of Lactobacillus casei Zhang...
  58. ncbi Control genes in quantitative molecular biological techniques: the variability of invariance
    S R Stürzenbaum
    Cardiff University, School of Biosciences BIOSI 2, P O Box 911, Wales, Cardiff, UK
    Comp Biochem Physiol B Biochem Mol Biol 130:281-9. 2001
    ..Finally, different approaches undertaken to assess the invariance of control genes are critically analysed to generate a procedure that will help to discern the best control for novel experiments...
  59. pmc Identification of miR-23a as a novel microRNA normalizer for relative quantification in human uterine cervical tissues
    Yuanming Shen
    Women s Reproductive Health Laboratory of Zhejiang Province
    Exp Mol Med 43:358-66. 2011
    ..Our results suggested that miR-23a and miR-191 are the optimal reference microRNAs that can be used for normalization in profiling studies of cervical tissues; miR-23a is a novel microRNA normalizer...
  60. doi Reference gene selection for quantitative real-time polymerase chain reaction in Populus
    Meng Xu
    Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing 210037, China
    Anal Biochem 408:337-9. 2011
    ....
  61. ncbi Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR
    T D Schmittgen
    Department of Pharmaceutical Sciences, College of Pharmacy, and the Cancer Prevention and Research Center, Washington State University, Pullman, WA 99164 6534, USA
    J Biochem Biophys Methods 46:69-81. 2000
    ..The internal control gene needs to be properly validated when designing quantitative gene expression studies...
  62. pmc Utility of composite reference standards and latent class analysis in evaluating the clinical accuracy of diagnostic tests for pertussis
    Andrew L Baughman
    National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30329, USA
    Clin Vaccine Immunol 15:106-14. 2008
    ..The accuracy of PCR in this study was evaluated using three reference standards: culture, CRS, and LCA...
  63. pmc Identification of reference genes across physiological states for qRT-PCR through microarray meta-analysis
    Wei Chung Cheng
    Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan
    PLoS ONE 6:e17347. 2011
    ..This study aimed to identify reference genes for clinical studies through microarray meta-analysis of human clinical samples...
  64. pmc "GOLD or lower limit of normal definition? A comparison with expert-based diagnosis of chronic obstructive pulmonary disease in a prospective cohort-study"
    Gülmisal Güder
    Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht, The Netherlands
    Respir Res 13:13. 2012
    ..We wanted to assess the diagnostic accuracy and prognostic capability of the GOLD and LLN definition when compared to an expert-based diagnosis...
  65. doi Range of protein detection by selected/multiple reaction monitoring mass spectrometry in an unfractionated human cell culture lysate
    H Alexander Ebhardt
    Department of Biology, Institute of Molecular Systems Biology, Eidgenossische Technische Hochschule ETH Zurich, Zurich, Switzerland
    Proteomics 12:1185-93. 2012
    ....
  66. doi Reference gene validation for qPCR on normoxia- and hypoxia-cultured human dermal fibroblasts exposed to UVA: is β-actin a reliable normalizer for photoaging studies?
    F Bruge
    Dipartimento Scienze Cliniche Specialistiche ed Odotontostomatologiche, Universita Politecnica delle Marche, I 60131 Ancona, Italy
    J Biotechnol 156:153-62. 2011
    ..These reference genes are hence recommended for future qPCR analyses in studies concerning photo-damage on UVA-treated HDF...
  67. doi Stability of reference proteins in human placenta: general protein stains are the benchmark
    D Lanoix
    INRS Institut Armand Frappier, Universite du Quebec, 531 Boulevard des Prairies, Laval, QC H7V 1B7, Canada
    Placenta 33:151-6. 2012
    ..The present study demonstrated the importance of using stable reference protein markers and normalization strategy in order to get correct results in semi-quantitative Western blot experiments in placental tissues...
  68. doi Selection of reference genes for gene expression studies in rats
    Roberto Martínez-Beamonte
    Departamento de Bioquimica y Biologia Molecular y Celular, Facultad de Veterinaria, Instituto de Investigación Sanitaria de Aragón Universidad de Zaragoza, Spain
    J Biotechnol 151:325-34. 2011
    ..Therefore, BestKeeper is highly recommendable to select the most stable gene to be used as internal standard and the selection of a specific reference expression gene requires a validation for each tissue and experimental design...
  69. doi Getting real with real-time qPCR: a case study of reference gene selection for morphological variation in Drosophila melanogaster wings
    Bruna P Matta
    Laboratório de Evolução de Caracteres Complexos, Departamento de Genetica, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rua Professor Rodolpho Paulo Rocco, s n Prédio do CCS, Bloco A, sala A2 075 Cidade Universitária, Rio de Janeiro, 21941 617, RJ, Brazil
    Dev Genes Evol 221:49-57. 2011
    ....
  70. pmc A method to extract cytokines and matrix metalloproteinases from Schirmer strips and analyze using Luminex
    Karl R VanDerMeid
    Pharmaceutical R and D, Bausch Lomb, Inc, Rochester, NY 14609, USA
    Mol Vis 17:1056-63. 2011
    ..This report describes a procedure which can be used to recover tears from the Schirmer strip for the measurement of multiple tear cytokines as well as matrix metalloproteinases (MMPs) by Luminex technology...
  71. pmc Evaluation of suitable reference genes for gene expression studies in porcine alveolar macrophages in response to LPS and LTA
    Mehmet Ulas Cinar
    Institute of Animal Sciences, Unit of Animal Breeding and Husbandry, University of Bonn, Endenicher Allee 15, 53115 Bonn, Germany
    BMC Res Notes 5:107. 2012
    ..Three different algorithms (geNorm, Normfinder and BestKeeper) were applied to assess the stability of HKGs...
  72. ncbi Empirical evidence of design-related bias in studies of diagnostic tests
    J G Lijmer
    Department of Clinical Epidemiology and Biostatistics, Academic Medical Center, University of Amsterdam, The Netherlands
    JAMA 282:1061-6. 1999
    ..The literature contains a large number of potential biases in the evaluation of diagnostic tests. Strict application of appropriate methodological criteria would invalidate the clinical application of most study results...
  73. doi Reference genes for the normalization of gene expression in eucalyptus species
    Luisa Abruzzi de Oliveira
    Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 91 501 970, Brazil
    Plant Cell Physiol 53:405-22. 2012
    ..Hence, novel Eucons04, Eucons08 and Eucons21 genes are the best suitable references for the normalization of expression studies in the Eucalyptus genus...
  74. doi Identification and testing of superior reference genes for a starting pool of transcript normalization in Arabidopsis
    Sung Myun Hong
    Creative Research Initiatives, School of Life Sciences and Biotechnology, Korea University, Seoul 136 701, Korea
    Plant Cell Physiol 51:1694-706. 2010
    ..We recommend the use of these genes as a starting pool for the identification of suitable reference genes under given experimental conditions to ensure accurate normalization in qRT-PCR analysis...
  75. ncbi Quantitative bioanalytical methods validation and implementation: best practices for chromatographic and ligand binding assays
    C T Viswanathan
    Food and Drug Administration, Center for Drug Evaluation and Research, Rockville, Maryland 20857, USA
    Pharm Res 24:1962-73. 2007
    ..This communication represents the distillate of the workshop proceedings and provides the summary of consensus reached and also contains the validation topics where no consensus was reached...
  76. pmc Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR
    Kang Zhou
    Chemical and Pharmaceutical Engineering, Singapore MIT Alliance, 4 Engineering Drive 3, Singapore
    BMC Mol Biol 12:18. 2011
    ..The objective of this study is to identify a set of reliable reference genes for transcription analysis in recombinant protein over-expression studies in E. coli...
  77. pmc Optimization of multiplexed bead-based cytokine immunoassays for rat serum and brain tissue
    R E Hulse
    Department of Neurology, The University of Chicago, MC2030, 5841 South Maryland Avenue, Chicago, IL 60637, USA
    J Neurosci Methods 136:87-98. 2004
    ..Furthermore, we describe animal handling procedures that minimize stress as determined by serum glucocorticoid levels since they can influence cytokine expression...
  78. pmc A review of the challenge in measuring hemoglobin A1c
    Cas Weykamp
    Queen Beatrix Hospital, Queen Beatrixpark 1, The Netherlands
    J Diabetes Sci Technol 3:439-45. 2009
    ..The implementation of this consensus statement raised new questions, to be answered in a concerted action of clinicians, biochemists, external quality assessment organizers, patient groups, and manufacturers...
  79. doi Reference genes for the relative quantification of microRNAs in renal cell carcinomas and their metastases
    Zofia Wotschofsky
    Department of Biology, Chemistry and Pharmacy, Free University, Berlin, Germany
    Anal Biochem 417:233-41. 2011
    ..RNU6B most frequently used as normalizer in miRNA expression studies should be abandoned in order to avoid misleading results...
  80. doi The use of microRNAs as reference genes for quantitative polymerase chain reaction in soybean
    Franceli Rodrigues Kulcheski
    Centre of Biotechnology, Laboratory of Genomes and Plant Population, Federal University of Rio Grande do Sul UFRGS, CEP 91501 970, Porto Alegre, RS, Brazil
    Anal Biochem 406:185-92. 2010
    ..Furthermore, these miRNAs genes were more stable than the most commonly protein-coding genes used in soybean gene expression studies involving RT-qPCR...
  81. doi Determination of volatile organic compounds in exhaled breath of patients with lung cancer using solid phase microextraction and gas chromatography mass spectrometry
    Magdalena Ligor
    Department of Anaesthesiology and Critical Care Medicine, Innsbruck Medical University, Innsbruck, Austria
    Clin Chem Lab Med 47:550-60. 2009
    ..This might lead to a future diagnostic test for lung cancer...
  82. doi A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
    Eduardo H C Farias
    Laboratório de Biotecnologia de Polímeros Naturais, Departamento de Bioquimica, Universidade Federal do Rio Grande do Norte, Natal, RN, 59072 970, Brazil
    Glycobiology 18:250-9. 2008
    ..A comparison of sulfated galactans from different marine taxonomic groups revealed similar backbones of 3-beta-D-Galp-1...
  83. doi Identification of suitable reference genes for gene expression studies of human serous ovarian cancer by real-time polymerase chain reaction
    Yan Li Li
    Women s Reproductive Health Laboratory of Zhejiang Province, Women s Hospital, School of Medicine, Zhejiang University, Xueshi Rd No 2, Hangzhou 310006, China
    Anal Biochem 394:110-6. 2009
    ....
  84. doi Identification of reference genes suitable for qRT-PCR in grapevine and application for the study of the expression of genes involved in pterostilbene synthesis
    Magdalena Gamm
    Unité Mixte de Recherche INRA 1088 CNRS 5184, Université de Bourgogne Plante Microbe Environnement, 17 rue Sully, BP 86510, 21065 Dijon Cedex, France
    Mol Genet Genomics 285:273-85. 2011
    ..cinerea, respectively. Next, we have highlighted that the expression of ROMT is induced in P. viticola-infected leaves and also in B. cinerea-infected berries, confirming the involvement of pterostilbene in grapevine defenses...
  85. ncbi Comparative assessment of large-scale data sets of protein-protein interactions
    Christian von Mering
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69012 Heidelberg, Germany
    Nature 417:399-403. 2002
    ..To measure their accuracy and potential as well as to identify biases, strengths and weaknesses, we compare the methods with each other and with a reference set of previously reported protein interactions...
  86. pmc Validation of reference genes for quantitative RT-PCR studies of gene expression in perennial ryegrass (Lolium perenne L.)
    Julia M Lee
    DairyNZ Ltd, Private Bag 3221, Hamilton 3240, New Zealand
    BMC Mol Biol 11:8. 2010
    ..Reference genes that have stable expression at different biological and physiological states can be effectively used for normalisation; however, their expression stability must be validated before use...
  87. doi Benchmarking next-generation transcriptome sequencing for functional and evolutionary genomics
    John G Gibbons
    Department of Biological Sciences, Vanderbilt University, Nashville, TN, USA
    Mol Biol Evol 26:2731-44. 2009
    ....
  88. doi Normalizing genes for real-time polymerase chain reaction in epithelial and nonepithelial cells of mouse small intestine
    Fengchao Wang
    Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Road, Shapingba District, Chongqing 400038, China
    Anal Biochem 399:211-7. 2010
    ..Thus, this study demonstrates that GUSB and TBP are the optimal reference genes for the normalization of gene expression in the mouse small intestine...
  89. pmc Potential of an age adjusted D-dimer cut-off value to improve the exclusion of pulmonary embolism in older patients: a retrospective analysis of three large cohorts
    Renee A Douma
    Department of Vascular Medicine, Academic Medical Centre, Meibergdreef 9, 1105 AZ, Amsterdam, Netherlands
    BMJ 340:c1475. 2010
    ..In older patients, the the D-dimer test for pulmonary embolism has reduced specificity and is therefore less useful. In this study a new, age dependent cut-off value for the test was devised and its usefulness with older patients assessed...
  90. ncbi Housekeeping proteins: a preliminary study illustrating some limitations as useful references in protein expression studies
    Roisean E Ferguson
    Cancer Research UK Clinical Centre, St James s University Hospital, UK
    Proteomics 5:566-71. 2005
    ....
  91. pmc Recommendations of the wwPDB NMR Validation Task Force
    Gaetano T Montelione
    Center for Advanced Biotechnology and Medicine, Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA Electronic address
    Structure 21:1563-70. 2013
    ..This paper summarizes the recommendations of the NMR-VTF, and lays the groundwork for future work in developing standards and metrics for biomolecular NMR structure quality assessment...
  92. ncbi Housekeeping genes as internal standards in cancer research
    Nico Janssens
    Department of Biochemistry, University of Antwerp, Wilrijk, Belgium
    Mol Diagn 8:107-13. 2004
    ..Therefore, it is necessary to characterize the suitability of various housekeeping genes to serve as internal RNA controls under particular experimental conditions where transcription effects are being tested...
  93. pmc Prerequisites for cytokine measurements in clinical trials with multiplex immunoassays
    Wilco de Jager
    Department of Pediatric Immunology, Centre for Molecular and Cellular Intervention CMCI, University Medical Centre Utrecht, Utrecht, The Netherlands
    BMC Immunol 10:52. 2009
    ..Therefore we set out to investigate several processes that might impact cytokine profiles such as blood collecting tubes, duration of storage, and number of freeze thawing cycles...
  94. pmc Avoiding pitfalls of internal controls: validation of reference genes for analysis by qRT-PCR and Western blot throughout rat retinal development
    Maurício Rocha-Martins
    Neurobiology Department, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
    PLoS ONE 7:e43028. 2012
    ....
  95. doi HPLC-MS method for the quantification of nine anti-HIV drugs from dry plasma spot on glass filter and their long term stability in different conditions
    Antonio D'Avolio
    Laboratory of Clinical Pharmacology and Pharmacogenetics, Department of Infectious Diseases, University of Torino, Amedeo di Savoia Hospital, Corso Svizzera 164, 10149 Turin, Italy
    J Pharm Biomed Anal 52:774-80. 2010
    ....
  96. pmc Evaluation of reference genes for real-time RT-PCR expression studies in the plant pathogen Pectobacterium atrosepticum
    Gunnhild W Takle
    Norwegian Institute for Agricultural and Environmental Research, Plant Health and Plant Protection Division, Høgskoleveien 7, 1432 As, Norway
    BMC Plant Biol 7:50. 2007
    ....
  97. ncbi Bone mineral density reference standards in adult baboons (Papio hamadryas) by sex and age
    L M Havill
    Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX 78245 0549, USA
    Bone 33:877-88. 2003
    ..As such, it provides much-needed reference standards that will allow researchers to evaluate the status of their animals in cross-sectional studies and more ..
  98. pmc Comparison of two reference standards in validating two field mydriatic digital photography as a method of screening for diabetic retinopathy
    P H Scanlon
    Gloucestershire Eye Unit, Cheltenham General Hospital, Sandford Road, Cheltenham GL53 7AN, UK
    Br J Ophthalmol 87:1258-63. 2003
    To compare two reference standards when evaluating a method of screening for referable diabetic retinopathy.
  99. pmc Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer
    Lise Aa Sørby
    Quality and Research Department, Ostfold Hospital Trust, 1603 Fredrikstad, Norway
    J Exp Clin Cancer Res 29:144. 2010
    ..The aim of this study was to validate a standardized experimental setup to identifying reference genes for normalization of qRT-PCR in the metastatic and non-metastatic colon cancer...
  100. pmc The Units Ontology: a tool for integrating units of measurement in science
    Georgios V Gkoutos
    Department of Computer Science, University of Aberystwyth, Old College, King Street, SY23 2AX
    Database (Oxford) 2012:bas033. 2012
    ..Here, we present the Units Ontology (UO), an ontology currently being used in many scientific resources for the standardized description of units of measurements...
  101. doi A liquid-liquid LC/MS/MS assay for the determination of artemether and DHA in malaria patient samples
    Lubbe Wiesner
    Department of Pharmacology, University of Cape Town, Medical School, Observatory, 7925 Cape Town, South Africa
    J Pharm Biomed Anal 55:373-8. 2011
    ....

Research Grants83

  1. Discovery metabolite profiling of the prolyl peptidases
    Alan Saghatelian; Fiscal Year: 2009
    ..Furthermore, the application of DMP to peptidases will demonstrate the generality of this approach for the future characterization of medically relevant enzymes and signaling pathways. ..
  2. Novel TIRF microscopy analyzing trafficking & signaling at the cell cortex
    Derek Toomre; Fiscal Year: 2009
    ..Consistent with the recovery act's goal, this funding will provide full-time summer employment for 4 undergraduate students and accelerate scientific achievement of the parent DP2 award...
  3. High Throughput Genotyping and DNA Sequencing for Studying the Genetic Contributi
    Sonia Dua; Fiscal Year: 2013
    ..The contractor is responsible for providing staff with the credentials required by CLIA. ..
  4. National Network of Libraries of Medicine (NN/LM) Region 2
    DENNIS PATTRATH; Fiscal Year: 2010
    ..abstract_text> ..
  5. National Network of Libraries of Medicine (NN/LM) Region 2
    Javier Crespo; Fiscal Year: 2010
    ..abstract_text> ..
  6. CFI Pathogen Inactivation Technology
    Trevor P Castor; Fiscal Year: 2010
    ..In addition to human plasma and human plasma proteins such as fibrinogen, the developed technology will also be applicable to recombinant therapeutics, monoclonal antibodies, transgenics and vaccines. ..
  7. National Network of Libraries of Medicine (NN/LM) Region 3
    Ruth Holst; Fiscal Year: 2010
    ..abstract_text> ..
  8. National Network of Libraries of Medicine (NN/LM) Region 3
    Ruth Holst; Fiscal Year: 2009
    ....
  9. National Network of Libraries of Medicine (NN/LM) region 4
    Claire Hamasu; Fiscal Year: 2010
    ..abstract_text> ..
  10. National Network of Libraries of Medicine (NN/LM) region 4
    Claire Hamasu; Fiscal Year: 2009
    ....
  11. National Network of Libraries of Medicine (NN/LM) Region 5
    MICHELLE MALIZIA; Fiscal Year: 2010
    ..abstract_text> ..
  12. Air Pollution on Adiposity and Vascular Dysfunction: White or Brown Matters?
    Qinghua Sun; Fiscal Year: 2013
    ....
  13. National Network of Libraries of Medicine (NN/LM) Region 5
    MICHELLE MALIZIA; Fiscal Year: 2009
    ....
  14. National Network of Libraries of Medicine (NN/LM) Region 6
    CATHY BURROUGHS; Fiscal Year: 2009
    ....
  15. National Network of Libraries of Medicine (NN/LM) Region 7
    TAMARA COMBS; Fiscal Year: 2010
    ..abstract_text> ..
  16. Polycyclic aromatic hydrocarbons and birth outcomes in Mexico City
    MARIE SYLVIA O'NEILL; Fiscal Year: 2013
    ....
  17. National Network of Libraries of Medicine (NN/LM) Region 1
    Colleen Cuddy; Fiscal Year: 2010
    ..abstract_text> ..
  18. National Network of Libraries of Medicine (NN/LM) Region 1
    KATE BURR OLIVER; Fiscal Year: 2009
    ....
  19. Bi-functional Therapeutics for Allergy-BFTA
    Swey Shen Chen; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE: The Project of bi-functional pan-IgE therapeutics may yield a commercializable product for treating IgE-mediated allergic diseases of different indications. ..
  20. A MicroRNA Approach to Screen for Sporadic Colon Cancer Exfoliately in Human Stoo
    Farid E Ahmed; Fiscal Year: 2010
    ..However, larger well designed clinical studies are needed to corroborate initial results. ..
  21. Iron in the pathogenesis of Friedreich's ataxia
    ARNULF HANS WERNER KOEPPEN; Fiscal Year: 2013
    ..The work is clinically relevant because it will resolve questions about iron in the formal pathogenesis and natural history of FRDA, and the potential value of iron chelation. ..
  22. Predicting Bilirubin-induced Auditory Toxicity in Infants with Severe Jaundice
    Sanjiv B Amin; Fiscal Year: 2010
    ....
  23. Air Pollution on Adiposity and Vascular Dysfunction: White or Brown Matters?
    Qinghua Sun; Fiscal Year: 2010
    ..abstract_text> ..
  24. PS11-003 Minority AIDS Research Initiative
    BRIDGETTE MERCEDEZ BRAWNER; Fiscal Year: 2013
    ..Given that adolescent health is imperative to public health, the proposed research is timely, innovative and bears great significance. ..
  25. Fast accurate ultrasensitive in vitro assay to determine BoNT potency &stability
    ARMIN H REITMAIR; Fiscal Year: 2012
    ....
  26. Role of Physico-chemical Properties in the Reprotoxicity of Inhaled Cd NP.
    JUDITH TERRY ZELIKOFF; Fiscal Year: 2010
    ....
  27. Air Toxics, Neighborhood Environment and Risk of Oral Clefts
    Philip J Lupo; Fiscal Year: 2013
    ..Ultimately, the identification of CL[unreadable]P risk factors will open the door to new strategies for lowering the prevalence of these significant conditions. ..
  28. Air Pollution, Inflammation and Preterm Birth: A Mechanistic Study in Mexico City
    MARIE SYLVIA O'NEILL; Fiscal Year: 2012
    ..abstract_text> ..
  29. Quantitation of Tocopherol Metabolism in Humans
    ANDREW JOSEPH CLIFFORD; Fiscal Year: 2010
    ..Particular attention will be directed to the absorption phase. The results are expected to provide information about vitamin E biopotency and eventually improved Dietary Recommendations. ..
  30. CT EIP Capacities ACA Grant
    TRACEY L WEEKS; Fiscal Year: 2011
    ....
  31. Adipocyte Renin-Angiotensin System and Programming of Hypertension
    Mina Desai; Fiscal Year: 2010
    ..Our proposed studies will therefore determine the role of adipose renin-angiotensin system in development of adult hypertension and obesity in growth restricted newborns. ..
  32. Osteoarthritis: Identification of Novel Imaging Biomarkers via 23Na/1H MRI at 7 T
    Gregory Chang; Fiscal Year: 2013
    ..changes in 7 T MR Measures of cartilage and bone are indicative of disease progression as assessed by reference standards (radiographs, conventional MRI, and clinical questionnaires)...
  33. Depot calcineurin inhibitors to prevent graft rejection
    Thomas J Smith; Fiscal Year: 2010
    ..In Phase 2 we propose to optimize the parameters of the delivery system, manufacture it according to FDA standards, and confirm its delivery profile and safety in further animal studies. ..
  34. Biomarkers of Sudden Death and Progressive Heart Failure
    GORDON FRANK TOMASELLI; Fiscal Year: 2010
    ....
  35. A Mindfulness-Based Approach to the Treatment of Obesity and Diabetes
    Carla K Miller; Fiscal Year: 2010
    ..The findings from this study will enable educators and clinicians to develop more effective education programs for adults with diabetes in both clinical and community settings. ..
  36. Nanodiagnostics and Nanotherapeutics: Building Research Ethics and Oversight
    Susan M Wolf; Fiscal Year: 2010
    ..abstract_text> ..
  37. CFI Inactivation of Human Plasma
    TREVOR CASTOR; Fiscal Year: 2009
    ..This approach is consistent with the regulatory authorities in Europe and the US that require a minimum of two virus inactivation technologies, which work by different mechanisms of action. ..
  38. QUANTITATIVE ANALYSIS OF AGING RETINA
    Christine A Curcio; Fiscal Year: 2012
    ....
  39. Bioconductor: an open computing resource for genomics
    Martin Morgan; Fiscal Year: 2010
    ..While that type of "success measure" is hard to assess, more effort in this area in subsequent proposals would be helpful. ..
  40. Bisphenol A: Urine flow disorder and prostate pathology
    FREDERICK S contact VOM SAAL; Fiscal Year: 2010
    ..Relating these diseases to estrogen exposure are likely to generate new therapeutic approaches based on blocking estrogen actions in the male urogenital system. ..
  41. Identifying Biomarkers for Post-Partum Depression in African-American Women
    Patrick F Sullivan; Fiscal Year: 2013
    ..Future research could evaluate whether a biomarker signature is of predictive utility and, if so, rational primary prevention may become feasible. ..
  42. Improving Air Quality for Laboratory Animal Research
    Sharron S Quisenberry; Fiscal Year: 2010
    ..A Project Liaison Officer will assist with communications and coordination among the key scientists and other Key Personnel. ..
  43. Robust BCT for Clinical Use
    DAVID KOPPERDAHL; Fiscal Year: 2010
    ..N. Diagnostics'business, and result in an important advance in the preventative care and treatment of osteoporosis. ..
  44. International Workshop on Opportunistic Protists (IWOP-12, 13 and 14)
    Louis M Weiss; Fiscal Year: 2012
    ..Although the opportunistic protists are difficult to study significant advances have resulted from the scientific interactions facilitated by the bi-annual IWOP meetings. ..
  45. Animal Models Workshop: The Physiology of Weight Loss Surgery
    LEE MICHAEL KAPLAN; Fiscal Year: 2011
    ..The workshop will include a two- and-one-half day scientific program, followed by a half-day series of live demonstrations of Roux-en-Y gastric bypass and other bariatric procedures in rat and mouse models. ..
  46. Phenotypes of diabetic kidney disease and the relationship to retinal pathology
    Amy Mottl; Fiscal Year: 2013
    ..This will form the basis for an R01 which will extend these robust techniques to a larger cohort and provide definitive data regarding the phenotypes of diabetic complications of the kidney and eye and their relationship to each other. ..
  47. Rapid Heart Attack Detection using an AC Electrokinetic Device
    Rajaram Krishnan; Fiscal Year: 2011
    ....
  48. Aptamer probes for Epigenetic Peptide Targets
    MARK PATRICK MCPIKE; Fiscal Year: 2010
    ..Unlike genetic aberrations, epigenetic changes are potentially reversible by modulating the activities of the enzymes that stabilize or remodel the packing of DNA in chromosomes. ..
  49. Ozone Cardiovascular Effects in Genetically Susceptible People
    MARK WALTER FRAMPTON; Fiscal Year: 2012
    ..Understanding the effects of ozone on the heart and circulation can help establish appropriate air pollution standards, and provide strategies to protect the most susceptible people. ..
  50. Harmful effects of transfusion of older stored red cells: iron and inflammation
    Steven L Spitalnik; Fiscal Year: 2012
    ....
  51. Placental shape features, gestational timing and maternal and infant health
    Carolyn M Salafia; Fiscal Year: 2011
    ....
  52. Competetive Renewal of MSM: Multiscale Studies of Segmentation in Vertebrates
    Claudio D Stern; Fiscal Year: 2013
    ..Its emphasis on model and data share ability will promote efficient sharing of resources, tools and models among biomodelers and experimentalists, significantly reducing duplication of effort. ..
  53. Investigating gene x environment interaction using human exposures to O3 & LPS
    David B Peden; Fiscal Year: 2010
    ..Finally, these studies will also add to fundamental advances in our understanding of the pathophysiology of common human conditions. ..
  54. Quantitation of In Vivo Human Lutein Metabolism
    ANDREW JOSEPH CLIFFORD; Fiscal Year: 2010
    ..Therefore, the purpose of this study is to determine how the human body uses lutein and other related compounds. ..
  55. Development of a nanosecond pulsed electric field system to treat skin cancer
    RICHARD LEE NUCCITELLI; Fiscal Year: 2011
    ..The PulseCure uses ultrashort electrical pulses to trigger skin tumors to self- destruct. It offers a non-surgical therapy that may be a scar-free and could improve the quality of life for tens of thousands of dermatology patients. ..
  56. Sorbents for Toxic-Metal Removal in Pharmaceutical Development and Manufacture
    GIRISH SRINIVAS; Fiscal Year: 2013
    ..The carbons will be sold in canisters;pharmaceutical mixtures purified by eluting solutions through packed beds of the activated carbons. ..
  57. Convene the leaders of the multiple networks providing telehealth services in the
    STEPHEN BIGGIO; Fiscal Year: 2011
    ..1 Michael Gallelli, Boomer, Technology, and Health: Consumers Taking Charge, MIT Enterprise Forum Northwest, http://www.mitwa.org/sites/default/files/files/MITEF%20NW%20Boomers%20Technology%20and%20Health%20Report.pdf ..
  58. New Personal Sampling Methods that Conform to an Aerosol Deposition Convention
    DARRAH KAYE SLEETH; Fiscal Year: 2013
    ....
  59. Biomimetic Microsystem for High Throughput Evaluation of Engineered Nanomaterials
    Robert M Worden; Fiscal Year: 2010
    ....
  60. Tobacco Products and Atherosclerotic Disease
    Sanjay Srivastava; Fiscal Year: 2013
    ..PHS 398/2590 (Rev. 06/09) Page Continuation Format Page ..
  61. Processing and Preservation of Human Umbilical Cord Stem Cells for Cell Therapeut
    Alfred Bahnson; Fiscal Year: 2010
    ..This project will study how to maximize the stocks of these cells and ensure their safe use in cell therapy. ..
  62. Liquid Crystal-Based End-of-Service-Life-Indicator for Toluene Vapor
    BHARAT R ACHARYA; Fiscal Year: 2011
    ....
  63. Reliable Evaluation of Coronary Artery Disease using Myocardial BOLD MRI with CO2
    Rohan Dharmakumar; Fiscal Year: 2013
    ..These studies are expected to provide the fundamental scientific basis for a non-invasive and reliable imaging strategy for evaluating prognosis of patients with coronary artery disease. ..
  64. A Medical Home Pilot Evaluation: A Model for Comparative Effectiveness Research
    KIM DUNN DUNN; Fiscal Year: 2010
    ..Fourth, we will have a data set to compare safety net and private patients, treatments, and effectiveness to be used for comparative effectiveness research in community settings ..
  65. Variation in the Safety of Back Pain-Related Surgery
    Sohail K Mirza; Fiscal Year: 2010
    ....
  66. OpenLIS: The Development of a Clinical Laboratory Information System Using the Op
    ROGER ALLAN HUBBARD; Fiscal Year: 2010
    ..For example, Medsphere Systems Corporation (Carlsbad, CA) thrives as a provider of professional support for an open source electronic medical records system. We have the expertise and experience to do the same with OpenLIS. ..
  67. Integrating Patient Generated Family Health History from Varied EHR Entry Portals
    Jennifer S Haas; Fiscal Year: 2010
    ....
  68. Defining Interoperability Standards for PRO Assessments
    DERSHUNG YANG; Fiscal Year: 2010
    ..Significant efforts have been spent in defining other types of medical data by various standards organizations, providing many technical precedents that can guide our standards development efforts and mitigate technical risks. ..
  69. Development of ex vivo Expanded Megakaryocyte Progenitors for Platelet Recovery F
    Holger Karsunky; Fiscal Year: 2009
    ..Completion of these proposed goals should enable Cellerant to move this potential product close to initiate IND enabling pre-clinical toxicology studies and subsequently Phase I clinical trials. ..
  70. Pain Assessment & Intervention Needs (PAIN)
    Fern Fitzhenry; Fiscal Year: 2010
    ..We will also data mine for other implicit, previously unknown, useful information such as conditions that should trigger or escalate an alert for an acute pain management intervention. ..
  71. Ethical & Methodological Standards for Trials of Invasive Therapeutic Procedures
    Carol M Ashton; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE: The project will result in substantial and much-needed improvements in the methodological and ethical quality of trials performed to evaluate invasive therapeutic procedures in humans. ..
  72. Evidence-based Guidelines Affecting Policy, Practice and Stakeholders (E-GAPPS)
    Peter C Wyer; Fiscal Year: 2012
    ..E-GAPPS should have important positive effect on improving the quality, usefulness and effectiveness of scientifically informed clinical guidelines in improving the care of individual patients. ..
  73. Vector Surveillance Ontology and Vector Management Ontology
    Saul Lozano Fuentes; Fiscal Year: 2010
    ..Moore, Marlize Coleman and Sanika Chitari) with broad expertise in Information Systems / Decision Support Systems and mosquito- and tick-borne diseases. ..
  74. Proximal Prostate Fluids for Protein and miRNA Biomarkers
    Richard R Drake; Fiscal Year: 2010
    ..Defining the constituent proteins and cellular miRNAs, standardization of collection procedures and establishing stability and storage parameters will facilitate incorporation of EPS urine based assays into mainstream clinical use. ..
  75. Specialized Centers for Cell-Based Therapy for NHLBI an*
    Shelly Carter; Fiscal Year: 2009
    ..End of Abstract) ..
  76. Profiling Urine Glycosylation of PSA and other Glyco-Biomarkers in Prostate Cance
    Lewis Pannell; Fiscal Year: 2009
    ..Prostatic acid phosphatase, an earlier glycoprotein biomarker of prostate cancer, will be similarly studied. Analyses will be performed in urine, leading to the possibility of a self-administered test for PC. ..
  77. Enabling Electronic Prescribing and Enhanced Management of Controlled Medications
    Grant Carrow; Fiscal Year: 2009
    ....
  78. Broadly Neuroprotective Drug for TBI
    Fernando Gomez Pinilla; Fiscal Year: 2010
    ..We expect to generate valuable pre-clinical information that will entitle us to initiate the steps for seeking IND approval. ..
  79. Formulation and Standardization of a Sickle Cell Disease Drug for Clinical Trial
    Robert Swift; Fiscal Year: 2010
    ..Treatment options are few. There is a substantial unmet medical need for new safe and effective disease modifying drugs, like the one in this grant, to treat this disease. ..
  80. A Standard Animal Model for Articular Cartilage Repair
    Anthony Ratcliffe; Fiscal Year: 2010
    ....
  81. PILOT TESTING OF ELECTRONIC PRESCRIBING STANDARDS
    Douglas Bell; Fiscal Year: 2006
    ..RxNorm and the structured and codified Sig standards will be evaluated primarily through laboratory testing. These will be implemented as additions to the existing standards if they improve information delivery. ..
  82. SBIR PHASE I TOPIC #177: ENHANCING THE QUALITY OF INTER
    T Tanner; Fiscal Year: 2000
    ....
  83. DIGITAL HAND ATLAS IN ASSESSMENT OF SKELETAL DEVELOPMENT
    H K Huang; Fiscal Year: 2006
    ..These advantages combined with higher accuracy and fast turn around time in diagnosis would gain acceptance by both pediatric radiologists and other clinicians. ..