transition temperature

Summary

Summary: The temperature at which a substance changes from one state or conformation of matter to another.

Top Publications

  1. ncbi Effect of product temperature during primary drying on the long-term stability of lyophilized proteins
    Stéphanie Passot
    UMR 782 Genie et Microbiologie des Procedes Alimentaires, AgroParisTech, INRA, Thiverval Grignon, France
    Pharm Dev Technol 12:543-53. 2007
  2. pmc Evaluation of high-resolution melting analysis as a diagnostic tool to detect the BRAF V600E mutation in colorectal tumors
    Martin Pichler
    Department of Internal Medicine, Medical University of Graz, Auenbruggerplatz 15, 8036 Graz, Austria
    J Mol Diagn 11:140-7. 2009
  3. pmc One-step triplex high-resolution melting analysis for rapid identification and simultaneous subtyping of frequently isolated Salmonella serovars
    Josef Zeinzinger
    Austrian Agency for Health and Food Safety, Institute of Medical Microbiology and Hygiene, Vienna, Austria
    Appl Environ Microbiol 78:3352-60. 2012
  4. ncbi An integrated approach to extreme thermostabilization and affinity maturation of an antibody
    Audrey D McConnell
    AnaptysBio, Inc, 10421 Pacific Center Court, Suite 200, San Diego, CA 92121, USA
    Protein Eng Des Sel 26:151-64. 2013
  5. ncbi Rapid molecular identification of Listeria species by use of real-time PCR and high-resolution melting analysis
    Dazhi Jin
    Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China
    FEMS Microbiol Lett 330:72-80. 2012
  6. pmc High resolution melting analysis for rapid and sensitive EGFR and KRAS mutation detection in formalin fixed paraffin embedded biopsies
    Hongdo Do
    Molecular Pathology Research and Development Laboratory, Department of Pathology, Peter MacCallum Cancer Centre, Locked Bag 1, A Beckett St, Melbourne, Victoria 8006, Australia
    BMC Cancer 8:142. 2008
  7. ncbi Classification of Mycoplasma synoviae strains using single-strand conformation polymorphism and high-resolution melting-curve analysis of the vlhA gene single-copy region
    Nathan Jeffery
    CSIRO Livestock Industries, F D McMaster Laboratory Chiswick, Armidale, New South Wales 2350, Australia
    Microbiology 153:2679-88. 2007
  8. ncbi Rapid detection and differentiation of Newcastle disease virus by real-time PCR with melting-curve analysis
    H M Pham
    Department of Disease Control, Laboratory of Infectious Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan
    Arch Virol 150:2429-38. 2005
  9. pmc High-resolution melting analysis as a powerful tool to discriminate and genotype Pseudomonas savastanoi pathovars and strains
    Andrea Gori
    Laboratorio di Patologia Vegetale e Molecolare, Dipartimento di Biotecnologie Agrarie, Universita degli Studi di Firenze, Sesto Fiorentino, Firenze, Italy
    PLoS ONE 7:e30199. 2012
  10. pmc Ultrasensitive detection of drug-resistant pandemic 2009 (H1N1) influenza A virus by rare-variant-sensitive high-resolution melting-curve analysis
    Neng Chen
    Department of Pathology, Stanford University School of Medicine, Stanford, California, USA
    J Clin Microbiol 49:2602-9. 2011

Research Grants

Detail Information

Publications275 found, 100 shown here

  1. ncbi Effect of product temperature during primary drying on the long-term stability of lyophilized proteins
    Stéphanie Passot
    UMR 782 Genie et Microbiologie des Procedes Alimentaires, AgroParisTech, INRA, Thiverval Grignon, France
    Pharm Dev Technol 12:543-53. 2007
    ..to investigate the effect of performing primary drying at product temperatures below and above Tg' (glass transition temperature of the freeze-concentrated phase) on the long-term stability of lyophilized proteins...
  2. pmc Evaluation of high-resolution melting analysis as a diagnostic tool to detect the BRAF V600E mutation in colorectal tumors
    Martin Pichler
    Department of Internal Medicine, Medical University of Graz, Auenbruggerplatz 15, 8036 Graz, Austria
    J Mol Diagn 11:140-7. 2009
    ..Despite the expected decrease in sensitivity, HRM can reliably be applied in archival formalin-fixed, paraffin-embedded samples tissues...
  3. pmc One-step triplex high-resolution melting analysis for rapid identification and simultaneous subtyping of frequently isolated Salmonella serovars
    Josef Zeinzinger
    Austrian Agency for Health and Food Safety, Institute of Medical Microbiology and Hygiene, Vienna, Austria
    Appl Environ Microbiol 78:3352-60. 2012
    ..These 39 serotypes represent more than 94% of all human and more than 85% of all nonhuman Salmonella isolates (including isolates from veterinary, food, and environmental samples) obtained in the years 2008 and 2009 in Austria...
  4. ncbi An integrated approach to extreme thermostabilization and affinity maturation of an antibody
    Audrey D McConnell
    AnaptysBio, Inc, 10421 Pacific Center Court, Suite 200, San Diego, CA 92121, USA
    Protein Eng Des Sel 26:151-64. 2013
    ....
  5. ncbi Rapid molecular identification of Listeria species by use of real-time PCR and high-resolution melting analysis
    Dazhi Jin
    Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China
    FEMS Microbiol Lett 330:72-80. 2012
    ..Consequently, this study demonstrates that the assay we developed is a functional tool for rapidly identifying six Listeria species, and has the potential for discriminating novel species food safety and epidemiological research...
  6. pmc High resolution melting analysis for rapid and sensitive EGFR and KRAS mutation detection in formalin fixed paraffin embedded biopsies
    Hongdo Do
    Molecular Pathology Research and Development Laboratory, Department of Pathology, Peter MacCallum Cancer Centre, Locked Bag 1, A Beckett St, Melbourne, Victoria 8006, Australia
    BMC Cancer 8:142. 2008
    ..However, up to now it has not been fully validated for clinical samples when formalin-fixed paraffin-embedded (FFPE) sections are the only material available for analysis as is often the case...
  7. ncbi Classification of Mycoplasma synoviae strains using single-strand conformation polymorphism and high-resolution melting-curve analysis of the vlhA gene single-copy region
    Nathan Jeffery
    CSIRO Livestock Industries, F D McMaster Laboratory Chiswick, Armidale, New South Wales 2350, Australia
    Microbiology 153:2679-88. 2007
    ..synoviae strains. In particular, the HRM curve analysis is a rapid and effective technique which can be performed in a single test tube in less than 2 h...
  8. ncbi Rapid detection and differentiation of Newcastle disease virus by real-time PCR with melting-curve analysis
    H M Pham
    Department of Disease Control, Laboratory of Infectious Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan
    Arch Virol 150:2429-38. 2005
    ..The total time for a PCR run was less than 1 hour. The results obtained in this study showed that the real-time PCR presented here is a good screening test for the identification of NDV...
  9. pmc High-resolution melting analysis as a powerful tool to discriminate and genotype Pseudomonas savastanoi pathovars and strains
    Andrea Gori
    Laboratorio di Patologia Vegetale e Molecolare, Dipartimento di Biotecnologie Agrarie, Universita degli Studi di Firenze, Sesto Fiorentino, Firenze, Italy
    PLoS ONE 7:e30199. 2012
    ..Its application is particularly suitable for sanitary certification programs for P. savastanoi, aimed at avoiding the spreading of this phytopathogen through asymptomatic plants...
  10. pmc Ultrasensitive detection of drug-resistant pandemic 2009 (H1N1) influenza A virus by rare-variant-sensitive high-resolution melting-curve analysis
    Neng Chen
    Department of Pathology, Stanford University School of Medicine, Stanford, California, USA
    J Clin Microbiol 49:2602-9. 2011
    ..Thus, patients naive to oseltamivir are most likely to be susceptible when this drug is used as a first-line treatment modality...
  11. ncbi Heat-transfer resistance at solid-liquid interfaces: a tool for the detection of single-nucleotide polymorphisms in DNA
    Bart van Grinsven
    Institute for Materials Research IMO, IMOMEC, Hasselt University, Wetenschapspark 1, B 3590 Diepenbeek, Belgium
    ACS Nano 6:2712-21. 2012
    ..Reference measurements by confocal fluorescence microscopy and impedance spectroscopy confirm that the switching of heat-transfer resistance upon denaturation is indeed related to the thermal on-chip denaturation of DNA...
  12. ncbi Rapid identification of multidrug-resistant Mycobacterium tuberculosis isolates by rpoB gene scanning using high-resolution melting curve PCR analysis
    Ariane T Pietzka
    Austrian Agency for Health and Food Safety, Vienna, Austria
    J Antimicrob Chemother 63:1121-7. 2009
    ..Rifampicin resistance caused by diverse mutations in the rpoB gene is an established and widely used surrogate marker for MDR-TB. We used a high-resolution melting (HRM) curve analysis approach to scan for mutations in the rpoB gene...
  13. pmc Rapid differentiation of influenza A virus subtypes and genetic screening for virus variants by high-resolution melting analysis
    Jih Hui Lin
    Center for Disease Control and Prevention, Taipei, Taiwan, Republic of China
    J Clin Microbiol 46:1090-7. 2008
    ..This assay requires no multiplexing or hybridization probes and provides a new approach for influenza A virus subtyping and genetic screening of virus variants in a clinical virology laboratory...
  14. ncbi Rapid detection and differentiation of the major mycoplasma contaminants in cell cultures using real-time PCR with SYBR Green I and melting curve analysis
    Ryo Harasawa
    Veterinary Microbiology, Department of Veterinary Medicine, Iwate University, Morioka, Iwate 020 8550, Japan
    Microbiol Immunol 49:859-63. 2005
    ..These results demonstrate that the protocol described in the present study can decrease the time to obtain reproducible results by simultaneous detection and differentiation of the Mycoplasma species contaminating cell cultures...
  15. pmc Isolation of a highly thermal stable lama single domain antibody specific for Staphylococcus aureus enterotoxin B
    Russell R Graef
    MITRE Corporation, McLean, Virginia 22102, USA
    BMC Biotechnol 11:86. 2011
    ..The ability of these small antigen-binding molecules to refold after heating to achieve their original structure, as well as their diminutive size, makes them attractive candidates for diagnostic assays...
  16. ncbi Dielectric relaxation spectroscopy of hydrated and dehydrated silk fibroin cast from aqueous solution
    Lei Yu
    Department of Physics and Astronomy and Biomedical Engineering Department, Tufts University, Medford, MA 02155, USA
    Biomacromolecules 11:2766-75. 2010
    ..During crystallization, α-helices can be transformed into antiparallel β-sheets, which possess no dipole moment, causing the decreasing trend in the dielectric parameters as crystallization proceeds...
  17. ncbi Context dependence of trinucleotide repeat structures
    Natalya N Degtyareva
    Department of Chemistry, Furman University, Greenville, South Carolina 29613, USA
    Biochemistry 49:3024-30. 2010
    ..Thus, an open (CAG)(8) loop and exposed bases in the arms indicate that the strand junction profoundly influences repeated sequences within three-way junctions...
  18. pmc Development of a rapid high-throughput method for high-resolution melting analysis for routine detection and genotyping of noroviruses
    Etsuko Tajiri-Utagawa
    Second Department of Virology, National Institute of Infectious Diseases, Musashimurayama shi, Tokyo, Japan
    J Clin Microbiol 47:435-40. 2009
    ..This assay requires no multiplexing or hybridization probes and provides a new approach to the genetic screening of noroviruses in clinical virology laboratories...
  19. ncbi Analysis of thermal melting curves
    Jean Louis Mergny
    Laboratoire de Biophysique, INSERM UR565, CNRS UMR 5153, Museum national d histoire naturelle, 75231 Paris, France
    Oligonucleotides 13:515-37. 2003
    ..We present the classic problems encountered during these experiments and try to demonstrate that a number of useful pieces of information can be extracted from these experimental curves...
  20. pmc Rapid differentiation of Mycobacterium tuberculosis and M. bovis by high-resolution melt curve analysis
    Suheir Ereqat
    Al Quds Nutrition and Health Research Institute, Faculty of Medicine, Al Quds University, Abu Deis, PO Box 201760, West Bank, Palestine
    J Clin Microbiol 48:4269-72. 2010
    ..This assay correctly identified the 7 tested Mycobacterium reference strains and 52 clinical samples with a sensitivity of 2 pg DNA. This assay will help in prescribing adequate treatment and monitoring disease dynamics...
  21. ncbi Towards an understanding of the release behavior of temperature-sensitive liposomes: a possible explanation of the "pseudoequilibrium" release behavior at the phase transition temperature
    Xin Zhang
    Department of Chemical Engineering, Imperial College London, South Kensington Campus, London, UK
    J Liposome Res 23:167-73. 2013
    ..2000) maintain a constant permeability to ionic molecules when they are heated to the phase transition temperature of the membrane (Tm). However, DPPC:DSPE...
  22. ncbi Advantages and limits of real-time PCR assay and PCR-restriction fragment length polymorphism for the identification of cutaneous Leishmania species in Tunisia
    Imène Ben Abda
    Research Laboratory of Emerging Parasitosis LR 05 SP 03, Pasteur Institute of Tunis, 13, Place Pasteur BP 74 1002 Tunis, Tunisia
    Trans R Soc Trop Med Hyg 105:17-22. 2011
    ..Both PCR assays can be used in a routine diagnostic, however, further prospective studies including largest sampling, are required to determine their performances in a routine use...
  23. ncbi A novel approach for analyzing glass-transition temperature vs. composition patterns: application to pharmaceutical compound+polymer systems
    Ioannis M Kalogeras
    Solid State Physics Section, Department of Physics, University of Athens, Panepistimiopolis, Zografos 157 84, Greece
    Eur J Pharm Sci 42:470-83. 2011
    ..encapsulation, as well as the physicochemical stability of the products, is often reflected on their glass transition temperature (T(g)) vs. composition (w) dependencies...
  24. pmc High-resolution melting approach to efficient identification and quantification of H275Y mutant influenza H1N1/2009 virus in mixed-virus-population samples
    Hong Kai Lee
    Molecular Diagnosis Center, National University of Singapore, Singapore, Republic of Singapore
    J Clin Microbiol 49:3555-9. 2011
    ....
  25. ncbi Influence of lipid composition on the phase transition temperature of liposomes composed of both DPPC and HSPC
    Jun Chen
    College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China
    Drug Dev Ind Pharm 39:197-204. 2013
    ..The objective of this study was to investigate the influence of molar ratio of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and hydrogenated soy phosphatidylcholine (HSPC) on the phase transition temperature (T(m)) of the liposomes.
  26. ncbi Rapid detection and non-subjective characterisation of infectious bronchitis virus isolates using high-resolution melt curve analysis and a mathematical model
    Kylie Hewson
    Department of Veterinary Science, The University of Melbourne, Vic, Australia
    Arch Virol 154:649-60. 2009
    ..This model is a rapid, reliable, accurate and non-subjective system for detection of IBVs in poultry flocks...
  27. ncbi Glass transition temperature of hard chairside reline materials after post-polymerisation treatments
    Vanessa M Urban
    Department of Dentistry, Ponta Grossa State University UEPG, Ponta Grossa, PR, Brazil
    Gerodontology 27:230-5. 2010
    This study evaluated the effect of post-polymerisation treatments on the glass transition temperature (T(g)) of five hard chairside reline materials (Duraliner II-D, Kooliner-K, New Truliner-N, Ufi Gel hard-U and Tokuso Rebase Fast-T).
  28. pmc Morphological behavior of lipid bilayers induced by melittin near the phase transition temperature
    Shuichi Toraya
    Graduate School of Engineering, Yokohama National University, Yokohama 240 8501, Japan
    Biophys J 89:3214-22. 2005
    ..On the other hand, additional large amplitude motion induced by melittin at a temperature near the Tc breaks down the magnetic alignment...
  29. ncbi Stabilization of low glass transition temperature indomethacin formulations: impact of polymer-type and its concentration
    Rina J Chokshi
    Applied Pharmaceutical Sciences, University of Rhode Island, Kingston, Rhode Island, USA
    J Pharm Sci 97:2286-98. 2008
    ..The stability of the amorphous form of INM could not be related to the glass transition temperature of the formulation as the mechanism of stabilization with EPO appears to be molecular interaction rather ..
  30. ncbi Determination of glass transition temperature and in situ study of the plasticizing effect of water by inverse gas chromatography
    Rahul Surana
    Department of Pharmaceutics, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Pharm Res 20:1647-54. 2003
    To use an inverse gas chromatographic (IGC) method to determine the glass transition temperature (Tg) of some amorphous pharmaceuticals and to extend this technique for the in situ study of the plasticizing effect of water on these ..
  31. ncbi Diisopropylammonium chloride: a ferroelectric organic salt with a high phase transition temperature and practical utilization level of spontaneous polarization
    Da Wei Fu
    Ordered Matter Science Research Center, Southeast University, Nanjing, 211196, P R China
    Adv Mater 23:5658-62. 2011
    A simple organic salt, diisopropylammonium chloride, shows the highest ferroelectric phase transition temperature among molecule-based ferroelectrics with a large spontaneous polarization, making it a candidate for practical technological ..
  32. ncbi Molecular-weight dependence of the glass transition temperature of freely-standing poly(methyl methacrylate) films
    C B Roth
    Department of Physics and the Guelph Waterloo Physics Institute, University of Guelph, N1G 2W1, Guelph, Ontario, Canada
    Eur Phys J E Soft Matter 20:441-8. 2006
    We have used transmission ellipsometry to measure the glass transition temperature, T(g), of freely-standing films of atactic and syndiotactic poly(methyl methacrylate) (PMMA)...
  33. ncbi Rapid identification of Listeria species and screening for variants by melting curve and high-resolution melting curve analyses of the intergenic spacer region of the rRNA gene
    Jun Wang
    Nippon Suisan Kaisha, Ltd, Food Safety Research Center, 559 6 Kitano Machi, Hachioji, Tokyo 192 0906, Japan
    Can J Microbiol 56:676-82. 2010
    ..In particular, the completion of both reaction and analysis in a closed tube saves time by eliminating the separate steps and lowers the risk of contamination...
  34. ncbi Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis
    Steven Y C Tong
    Menzies School of Health Research, Darwin, PO Box 41096 Casuarina, NT 0811, Australia
    Diagn Microbiol Infect Dis 67:399-401. 2010
    ..Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform...
  35. pmc Use of sloppy molecular beacon probes for identification of mycobacterial species
    Hiyam H El-Hajj
    Public Health Research Institute, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA
    J Clin Microbiol 47:1190-8. 2009
    ..Utilizing this general paradigm, screening assays can be designed for the identification of a wide range of species...
  36. ncbi High-resolution melting analysis of the spa locus reveals significant diversity within sequence type 93 methicillin-resistant Staphylococcus aureus from northern Australia
    S Y C Tong
    Menzies School of Health Research, Institute of Advanced Studies, Charles Darwin University, Darwin, NT, Australia
    Clin Microbiol Infect 15:1126-31. 2009
    ..aureus (MSSA), and the coexistence of MSSA and methicillin-resistant S. aureus versions of the same lineage within northern Australia...
  37. pmc aac(6')-Ib-cr genotyping by simultaneous high-resolution melting analyses of an unlabeled probe and full-length amplicon
    Jan M Bell
    SA Pathology, Women s and Children s Hospital, 72 King William Rd, North Adelaide, SA 5006, Australia
    Antimicrob Agents Chemother 54:1378-80. 2010
    ..Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon...
  38. ncbi Optimization of high-resolution melting analysis for low-cost and rapid screening of allelic variants of Bacillus anthracis by multiple-locus variable-number tandem repeat analysis
    Daniela Fortini
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    Clin Chem 53:1377-80. 2007
    ..Although the method has great differentiating power and reproducibility, faster genotyping at low cost may be requested to accurately identify B. anthracis strains in the field...
  39. pmc Classification of fowl adenovirus serotypes by use of high-resolution melting-curve analysis of the hexon gene region
    Penelope A Steer
    Faculty of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia
    J Clin Microbiol 47:311-21. 2009
    ....
  40. ncbi Small-molecule binding at an abasic site of DNA: strong binding of lumiflavin for improved recognition of thymine-related single nucleotide polymorphisms
    N B Sankaran
    Department of Chemistry, Graduate School of Science, Tohoku University, Aoba ku, Sendai 980 8578, Japan
    J Phys Chem B 113:1522-9. 2009
    ..In comparison to riboflavin, reported previously as a T-selective ligand, lumiflavin binds to the DNA much more strongly and is a more promising ligand for efficient detection of T-related single nucleotide polymorphisms...
  41. ncbi High-resolution melting analysis of the spa repeat region of Staphylococcus aureus
    Alex J Stephens
    Cooperative Research Centre for Diagnostics, Institute of Health and Biomedical Innovation, Queensland University of Technology, Queensland, Australia
    Clin Chem 54:432-6. 2008
    ..We describe here a method for genotyping S. aureus using high-resolution melting (HRM) analysis of the spa polymorphic region X...
  42. pmc Fluorometric assay for testing rifampin susceptibility of Mycobacterium tuberculosis complex
    K G P Hoek
    DST NRF Centre of Excellence for Biomedical Tuberculosis Research MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Health Sciences, Stellenbosch University, Tygerberg, Cape Town, South Africa
    J Clin Microbiol 46:1369-73. 2008
    ..This method, named "FAST-Rif" ("fluorometric assay for susceptibility testing of rifampin"), allowed the rapid, reliable, and easy detection of genotypic rifampin resistance as a marker for MDR-TB and XDR-TB...
  43. pmc Rapid universal identification of bacterial pathogens from clinical cultures by using a novel sloppy molecular beacon melting temperature signature technique
    Soumitesh Chakravorty
    Division of Infectious Disease, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, 185 South Orange Avenue, Newark, NJ 07103, USA
    J Clin Microbiol 48:258-67. 2010
    ..This approach enables highly multiplexed target detection using a simple PCR format that can transform infectious disease diagnostics and improve patient outcomes...
  44. ncbi Methylation-sensitive high-resolution melting-curve analysis of the SNRPN gene as a diagnostic screen for Prader-Willi and Angelman syndromes
    Helen E White
    National Genetics Reference Laboratory Wessex, Salisbury District Hospital, Odstock, Salisbury, Wiltshire, United Kingdom
    Clin Chem 53:1960-2. 2007
    ..Analysis of allelic methylation differences at the small nuclear ribonucleoprotein polypeptide N (SNRPN) locus differentiates the maternally and paternally inherited chromosome 15 and can be used as a diagnostic test for AS and PWS...
  45. ncbi Arginine-based PNA microarrays for APOE genotyping
    Alessandro Calabretta
    Department of Organic and Industrial Chemistry, University of Parma, Viale G P Usberti 17a, I 43100, Parma, Italy
    Mol Biosyst 5:1323-30. 2009
    ..Six different hybridisation patterns related to six different genotypes in the APOE gene were found to be clearly distinct in microarray experiments, demonstrating the potential of this approach for highly specific genetic analysis...
  46. ncbi Combining sequence-specific probes and DNA binding dyes in real-time PCR for specific nucleic acid quantification and melting curve analysis
    Kristina Lind
    Chalmers University of Technology, Gothenburg, Sweden
    Biotechniques 40:315-9. 2006
    ..The probe signal reflect formation of target product, while melting curve analysis of the BOXTO signal reveals primer-dimer formation and the presence of any other anomalous products...
  47. ncbi Synthesis of cell-permeable peptide nucleic acids and characterization of their hybridization and uptake properties
    Peng Zhou
    Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA
    Bioorg Med Chem Lett 16:4931-5. 2006
    ..Thermal denaturation study showed that GPNA oligomers containing alternate D-backbone configuration bind sequence-specifically to DNA and, when incubated with mammalian cells, localized specifically to the endoplasmic reticulum (ER)...
  48. pmc High resolution melting analysis for JAK2 Exon 14 and Exon 12 mutations: a diagnostic tool for myeloproliferative neoplasms
    Inmaculada Rapado
    Hematology Science, Hospital Universitario, Madrid, Spain
    J Mol Diagn 11:155-61. 2009
    ..Hence, HRM analysis of exons 14 and 12 in JAK2 shows better diagnostic values than three other routinely used methods against which it was compared. In addition, HRM analysis has the advantage of detecting unknown mutations...
  49. ncbi Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping
    Mark G Herrmann
    Institute for Clinical and Experimental Pathology, ARUP, Salt Lake City, UT 84108, USA
    Clin Chem 53:1544-8. 2007
    ..We assessed the performance of these new instruments for genotyping and scanning for mutations...
  50. ncbi Strand invasion of mixed-sequence B-DNA by acridine-linked, gamma-peptide nucleic acid (gamma-PNA)
    Srinivas Rapireddy
    Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    J Am Chem Soc 129:15596-600. 2007
    ..This finding is significant because it demonstrates that the same principles that guide the recognition of single-stranded DNA and RNA can also be applied to double-stranded B-DNA...
  51. ncbi Detection of the JAK2(V617F) mutation in myeloproliferative disorders by melting curve analysis using the LightCycler system
    Randall J Olsen
    Department of Pathology, Baylor College of Medicine, Houston, Texas, USA
    Arch Pathol Lab Med 130:997-1003. 2006
    ..A specific mutation, JAK2(V617F), was recently recognized as having diagnostic value for myeloproliferative disorders. No practical assay is currently available for routine use in a clinical laboratory...
  52. pmc Transport properties of glass-forming liquids suggest that dynamic crossover temperature is as important as the glass transition temperature
    Francesco Mallamace
    Dipartimento di Fisica and Consorzio Nazionale Interuniversitario per le Scienze Fisiche della Materia, Universita di Messina, I 98166 Messina, Italy
    Proc Natl Acad Sci U S A 107:22457-62. 2010
    ..The crossover temperature, T(×), located well above the calorimetric glass transition temperature T(g), marks significant variations in the system thermodynamics, evidenced by the change of the SA-like T ..
  53. ncbi High-resolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution
    Luming Zhou
    Department of Pathology, University of Utah Medical Center, Salt Lake City, UT 84105, USA
    Clin Chem 51:1770-7. 2005
    ..However, simultaneous PCR product scanning and probe genotyping in the same reaction has not been described...
  54. ncbi Using an aryl phenanthroimidazole moiety as a conjugated flexible intercalator to improve the hybridization efficiency of a triplex-forming oligonucleotide
    Amany M A Osman
    Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Bioorg Med Chem 16:9937-47. 2008
    ..The required monomers for DNA synthesis using amidite chemistry were obtained by standard deprotection of the hydroxy groups followed by 4,4'-dimethoxytritylation and phosphitylation...
  55. ncbi SYTO9 and SYBR GREEN1 with high resolution melting analysis for molecular confirmatory testing of the common Southeast Asian beta(0)-thalassemia mutations
    Unthika Chamras
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand 50200
    Hemoglobin 33:539-45. 2009
    ..The SYTO9 and SYBR GREEN1 HRM analysis is simple and rapid. This approach will facilitate laboratory diagnosis and genetic counseling for regions with a high prevalence of beta-thal...
  56. ncbi Influence of curing protocol on selected properties of light-curing polymers: degree of conversion, volume contraction, elastic modulus, and glass transition temperature
    Magali Dewaele
    Laboratory of Chemistry and Physics of High Polymers, Universite Catholique de Louvain, Louvain la Neuve, Belgium
    Dent Mater 25:1576-84. 2009
    ..light-curing protocol on degree of conversion (DC), volume contraction (C), elastic modulus (E), and glass transition temperature (T(g)) as measured on a model polymer...
  57. pmc High-throughput genotyping of single nucleotide polymorphisms in the Plasmodium falciparum dhfr gene by asymmetric PCR and melt-curve analysis
    Rochelle E Cruz
    School of Public Health, University of Alberta, Edmonton, Canada
    J Clin Microbiol 48:3081-7. 2010
    ..Our assay provides a number of technical improvements that facilitate the high-throughput screening of patient samples to identify SP-resistant malaria...
  58. pmc Low amounts of sucrose are sufficient to depress the phase transition temperature of dry phosphatidylcholine, but not for lyoprotection of liposomes
    Constança Cacela
    Max Planck Institut fur Molekulare Pflanzenphysiologie, D 14424 Potsdam, Germany
    Biophys J 90:2831-42. 2006
    ..that the lyoprotection of liposomes depends on the depression of the gel to liquid-crystalline phase transition temperature (T(m)) of the dry membranes below ambient and on the prevention of membrane fusion by sugar glass ..
  59. pmc Detection of the JAK2 V617F mutation by LightCycler PCR and probe dissociation analysis
    Marla Lay
    Molecular Pathology Laboratory, L235, 300 Pasteur Dr, Stanford University School of Medicine, CA 94305, USA
    J Mol Diagn 8:330-4. 2006
    ..We found that the LightCycler method offered advantages of speed, reliability, and more straightforward interpretation over the restriction fragment-length polymorphism and sequencing approaches...
  60. pmc Rescue of glaucoma-causing mutant myocilin thermal stability by chemical chaperones
    J Nicole Burns
    Georgia Institute of Technology, Atlanta, 30332, USA
    ACS Chem Biol 5:477-87. 2010
    ....
  61. ncbi Nanoencapsulation of a water soluble drug in biocompatible polyesters. Effect of polyesters melting point and glass transition temperature on drug release behavior
    Vassilios Karavelidis
    Laboratory of Organic Chemical Technology, Chemistry Department, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece
    Eur J Pharm Sci 41:636-43. 2010
    ..Melting point (T(m)) and glass transition temperature (T(g)) of the host polymer matrices seem to be important parameters, since higher drug release rates are ..
  62. ncbi Closed-tube SNP genotyping without labeled probes/a comparison between unlabeled probe and amplicon melting
    Michael Liew
    Institute for Clinical and Experimental Pathology, ARUP, Salt Lake City, UT 84108 1221, USA
    Am J Clin Pathol 127:341-8. 2007
    ..When 2 temperature standards were used to bracket the amplicon of interest, the accuracy of amplicon genotyping of SNPs was increased to 100% (LightTyper) and 88% (I-Cycler)...
  63. ncbi High-resolution melting assay for the detection of gyrA mutations causing quinolone resistance in Salmonella enterica serovars Typhi and Paratyphi
    Robert Slinger
    Division of Bacteriology, Department of Pathology and Laboratory Medicine, Children s Hospital of Eastern Ontario, Ottawa, ON, Canada K1H 8L1
    Diagn Microbiol Infect Dis 57:455-8. 2007
    ..The presence of gyrA mutations led to small but consistent changes in amplicon melting temperatures that allowed quinolone-resistant isolates to be differentiated from susceptible ones...
  64. pmc Interstrand DNA cross-links induced by alpha,beta-unsaturated aldehydes derived from lipid peroxidation and environmental sources
    Michael P Stone
    Department of Chemistry, Center in Molecular Toxicology, and Vanderbilt Institute for Chemical Biology, Vanderbilt University, Nashville, Tennessee 37235, USA
    Acc Chem Res 41:793-804. 2008
    ..Reduced derivatives of these cross-links are useful tools for studying their biological processing...
  65. pmc Mixture models for analysis of melting temperature data
    Christoffer Nellåker
    Department of Neuroscience, Karolinska Institutet, Retzius Väg, Stockholm, Sweden
    BMC Bioinformatics 9:370. 2008
    ..Methodological improvements in recent years allow the generation of high-resolution melting-temperature (Tm) data. However, there is currently no convention on how to statistically analyze such high-resolution Tm data...
  66. pmc Detection of the East and West African kdr mutation in Anopheles gambiae and Anopheles arabiensis from Uganda using a new assay based on FRET/Melt Curve analysis
    Katrijn Verhaeghen
    Departement of Parasitology, Prins Leopold Institute of Tropical Medicine, Nationalestraat 155, B 2000 Antwerp, Belgium
    Malar J 5:16. 2006
    ..Two different allele-specific polymerase chain reactions (AS-PCR) are needed to detect these known kdr mutations. However, these AS-PCR assays rely on a single nucleotide polymorphism mismatch, which can result in unreliable results...
  67. ncbi Molecular beacon-based temperature control and automated analyses for improved resolution of melting temperature analysis using SYBR I green chemistry
    Christoffer Nellåker
    Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
    Clin Chem 53:98-103. 2007
    ....
  68. pmc Analytical detection of immunoglobulin heavy chain gene rearrangements in gastric lymphoid infiltrates by peak area analysis of the melting curve in the LightCycler System
    Eduardo Retamales
    Departamento Laboratorio de Salud, Instituto Salud Publica de Chile, Santiago, Chile
    J Mol Diagn 9:351-7. 2007
    ....
  69. ncbi Comparison of different melting temperature calculation methods for short DNA sequences
    Alejandro Panjkovich
    Departamento de Genetica Molecular y Microbiologia, Facultad de Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Alameda 340, Santiago, Chile
    Bioinformatics 21:711-22. 2005
    ....
  70. pmc LightCycler technology in molecular diagnostics
    Elaine Lyon
    Department of Pathology, University of Utah, Salt Lake City, Utah, USA
    J Mol Diagn 11:93-101. 2009
    ..High-resolution melting allows mutation scanning by detecting all heterozygous changes. This review describes the major advances throughout the last 15 years regarding LightCycler technology and its application in clinical laboratories...
  71. ncbi Quantitative equivalence between polymer nanocomposites and thin polymer films
    Amitabh Bansal
    Department of Materials Science and Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA
    Nat Mater 4:693-8. 2005
    ..We show that the changes in glass-transition temperature with decreasing interparticle spacing for two filler surface treatments are quantitatively equivalent to ..
  72. ncbi Substitution of aspartic acid with glutamic acid increases the unfolding transition temperature of a protein
    Duck Yeon Lee
    Biomedical Research Center, Korea Institute of Science and Technology, Cheongyang Box 131, Seoul 130 650, Republic of Korea
    Biochem Biophys Res Commun 320:900-6. 2004
    ..5 degrees C. The change of Tm destabilizes the MjTRX by 0.55 kcal/mol and stabilizes the ETRX by 0.45 kcal/mol in free energy...
  73. ncbi Identification of trinucleotide repeat ligands with a FRET melting assay
    Samir Amrane
    Muséum National d Histoire Naturelle MNHN USM 503, INSERM U565, CNRS UMR 5153, Case Postale 26, 57 rue Cuvier 75005 Paris, France
    Chembiochem 9:1229-34. 2008
    ..Remarkably, we found that macrocyclic compounds, such as acridine dimers and trimers, exhibit interesting affinities and specificities for this motif...
  74. ncbi Energetic basis of molecular recognition in a DNA aptamer
    G Reid Bishop
    Department of Chemistry and Biochemistry, Mississippi College, 200 S Capitol St, Clinton, MS 39058, USA
    Biophys Chem 126:165-75. 2007
    ....
  75. ncbi Effect of physical properties of troglitazone crystal on the molecular interaction with PVP during heating
    Susumu Hasegawa
    Pharmaceutical Development Laboratories, Sankyo Co Ltd, 1 12 1 Shinomiya, Hiratsuka, Kanagawa 254 0014, Japan
    Int J Pharm 336:82-9. 2007
    ..PM) containing unmilled troglitazone must be heated above at least 50 degrees C higher than the glass transition temperature (T(g)) of PVP to obtain an SD showing 100% dissolution, while PMs containing milled troglitazone could be ..
  76. ncbi Organic glasses with exceptional thermodynamic and kinetic stability
    Stephen F Swallen
    Department of Chemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Science 315:353-6. 2007
    ..is optimized when deposition occurs on substrates at a temperature of 50 K below the conventional glass transition temperature. We attribute the substantial improvement in thermodynamic and kinetic properties to enhanced mobility ..
  77. ncbi Part I: characterization of solid dispersions of nimodipine prepared by hot-melt extrusion
    Xin Zheng
    Department of Pharmaceutics, Shenyang Pharmaceutical University, Shenyang, Liaoning, P R China
    Drug Dev Ind Pharm 33:791-802. 2007
    ..The weakening and shift of the N-H stretching vibration of the secondary amine groups of nimodipine as determined by FT-IR proved hydrogen bonding between the drug and polymers in the solid dispersion...
  78. ncbi Crystallization and phase behavior of fatty acid esters of 1,3 propanediol III: 1,3 propanediol dicaprylate/1,3 propanediol distearate (CC/SS) and 1,3 propanediol dicaprylate/1,3 propanediol dipalmitate (CC/PP) binary systems
    Madjid Abes
    Alberta Lipid Utilization Program, Department of Agricultural Food and Nutritional Science, 4 10 Agriculture Forestry Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2P5
    Chem Phys Lipids 151:110-24. 2008
    ..3 compared to the CC/SS mixtures were associated with the chain length difference...
  79. ncbi A calorimetric investigation of thermodynamic and molecular mobility contributions to the physical stability of two pharmaceutical glasses
    Deliang Zhou
    Department of Pharmaceutics, College of Pharmacy, University of Minnesota, Weaver Densford Hall, 308 Harvard Street SE, Minneapolis, MN 55455 0343, USA
    J Pharm Sci 96:71-83. 2007
    ..These observations below Tg are consistent with the previous work on physical stability of amorphous pharmaceuticals performed above Tg...
  80. ncbi Solid state chemistry of proteins: I. glass transition behavior in freeze dried disaccharide formulations of human growth hormone (hGH)
    Michael J Pikal
    School of Pharmacy, University of Connecticut, Storrs, Connecticut, USA
    J Pharm Sci 96:2765-76. 2007
    ..differential scanning calorimetry (DSC), a protein-rich system behaves as a "strong glass", and the glass transition temperature, T(g), cannot be directly determined by DSC...
  81. ncbi Enthalpy and interfacial free energy changes of water capillary condensed in mesoporous silica, MCM-41 and SBA-15
    Shigeharu Kittaka
    Department of Chemistry, Faculty of Science, Okayama University of Science, 1 1 Ridaicho, Okayama, 700 0005, Japan
    Phys Chem Chem Phys 8:3223-31. 2006
    ....
  82. ncbi Gelatinization and freeze-concentration effects on recrystallization in corn and potato starch gels
    Felicidad Ronda
    Departamento de Ingeniería Agrícola y Forestal, Tecnologia de los Alimentos, E T S Ingenierías Agrarias, Universidad de Valladolid, 34004 Palencia, Spain
    Carbohydr Res 343:903-11. 2008
    ..Freeze-concentration temperatures also showed a significant effect on the size and the perfection of crystals formed in starch recrystallization...
  83. ncbi Recrystallization of nifedipine and felodipine from amorphous molecular level solid dispersions containing poly(vinylpyrrolidone) and sorbed water
    Patrick J Marsac
    Department of Industrial and Physical Pharmacy, School of Pharmacy, Purdue University, West Lafayette, Indiana, 47907 USA
    Pharm Res 25:647-56. 2008
    ..To compare the physical stability of amorphous molecular level solid dispersions of nifedipine and felodipine, in the presence of poly(vinylpyrrolidone) (PVP) and small amounts of moisture...
  84. ncbi Study of thermal behaviour of sugar esters
    Angéla Szuts
    Department of Pharmaceutical Technology, University of Szeged, H 6720 Szeged, Eotvos u 6, Hungary
    Int J Pharm 336:199-207. 2007
    ..Time-dependent solid-state changes (crystalline and amorphous phases) were observed, and analysed by means of differential scanning calorimetry and X-ray powder diffraction...
  85. ncbi Gamma radiolysis of ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate
    Mingying Qi
    Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
    Radiat Res 167:508-14. 2007
    ..When the irradiated [bmim][PF6] samples were cooled, two crystal structures were found to coexist, and they suffered a continuous destruction under irradiation; their dose dependence, however, was different...
  86. ncbi Clathrate formation and dissociation in vapor/water/ice/hydrate systems in SBA-15, sol-gel and CPG porous media, as probed by NMR relaxation, novel protocol NMR cryoporometry, neutron scattering and ab initio quantum-mechanical molecular dynamics simulati
    J Beau W Webber
    Institute of Petroleum Engineering, Heriot Watt University, Edinburgh EH14 4AS, UK
    Magn Reson Imaging 25:533-6. 2007
    ..Ab initio quantum-mechanical molecular dynamics calculations are also being employed to probe the dynamics of liquids in pores at nanometric dimensions...
  87. ncbi Sudden rise of crystal growth rate of nifedipine near T(g) without and with polyvinylpyrrolidone
    Hajime Ishida
    School of Pharmacy, University of Wisconsin Madison, Madison, Wisconsin 53705, USA
    J Pharm Sci 96:1131-8. 2007
    ..in the beta polymorph has been measured from T(g) + 50 to T(g) - 20 K, where T(g) = 315 K is the glass transition temperature. A sudden 10-fold rise of growth rate was observed as the temperature decreased from above to below T(g) ..
  88. ncbi Crystal growth formation in melt extrudates
    Caroline Bruce
    College of Pharmacy, The University of Texas, Austin, TX 78712, United States
    Int J Pharm 341:162-72. 2007
    ..In vitro drug release studies showed no significant change during storage for up to 6 months at 25 degrees C/60% relative humidity and 40 degrees C/75% relative humidity...
  89. ncbi A comparative study of the effects of dimethylsulfoxide and glycerol on the bicontinuous cubic structure of hydrated monoolein and its phase behavior
    Satoru Abe
    Biophysics Laboratory, Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Aramaki 4 2, Maebashi 371 8510, Japan
    Chem Phys Lipids 147:59-68. 2007
    ..Glycerol shows opposite effects. The present study suggests that different mechanisms act in the cryopreservation by DMSO and glycerol...
  90. ncbi Differential scanning calorimetry (DSC) and temperature-modulated DSC study of three mouthguard materials
    Frank H Meng
    Section of Orthodontics, College of Dentistry, The Ohio State University, Columbus, OH 43218, USA
    Dent Mater 23:1492-9. 2007
    ..Employ differential scanning calorimetry (DSC) and temperature-modulated DSC (TMDSC) to investigate thermal transformations in three mouthguard materials and provide insight into their previously investigated energy absorption...
  91. ncbi Solubility of molecular crystals: polymorphism in the light of solubility theory
    P Bennema
    Synthon BV, Nijmegen, The Netherlands
    Int J Pharm 351:74-91. 2008
    ..In passing a new method is introduced that allows to estimate the transition temperature of enantiotropically related polymorphs from melting temperatures and enthalpies of the polymorphs.
  92. ncbi High pressure differential scanning calorimetry investigations on the pressure dependence of the melting of paracetamol Polymorphs I and II
    J Ledru
    School of Engineering and Physical Sciences, University of Aberdeen, Meston Building, Meston Walk, Aberdeen AB24 3UE, UK
    J Pharm Sci 96:2784-94. 2007
    ..7 MPa and T = 489.6 K) for the first time, and confirm those predicted by Espeau et al. from a topological p-T diagram based on theoretical arguments and experimental data at ambient pressure...
  93. ncbi Poly(vinyl alcohol)/silica nanocomposites: morphology and thermal degradation kinetics
    Zheng Peng
    Agriculture Ministry Key Laboratory of Natural Rubber Processing, Zhanjiang 524001, China
    J Nanosci Nanotechnol 6:3934-8. 2006
    ..However, at a given degradation temperature, the nanocomposite presents much lower reaction velocity constants (k), while its E is 20 kJ/mol higher than that of the PVA host...
  94. ncbi Effect of n-scCO(2) on crystalline to amorphous conversion of carbamazepine
    Shweta Ugaonkar
    Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, RI 02881, USA
    Int J Pharm 333:152-61. 2007
    ....
  95. ncbi Formulation, lyophilization and solid-state properties of a pegylated protein
    Mitra Mosharraf
    Product and Process Development, Pfizer Global Manufacturing, Pfizer, SE 112 87 Stockholm, Sweden
    Int J Pharm 336:215-32. 2007
    ..Pegylation caused a shorter dissolution time, lower moisture content, increased amorphicity, and a more rapid moisture-induced crystallization of sucrose...
  96. ncbi Differential scanning calorimetry
    Charles H Spink
    Department of Chemistry, State University of New York Cortland, Cortland, New York 13045, USA
    Methods Cell Biol 84:115-41. 2008
    ..The second example shows the application of DSC for the study of the unfolding of a simple hairpin oligonucleotide. Details of the data analysis are presented in a simple spreadsheet format...
  97. ncbi Corrosion behavior of shape memory, superelastic, and nonsuperelastic nickel-titanium-based orthodontic wires at various temperatures
    Deo K Pun
    Dental Biomaterials, School of Dentistry, Marquette University, Milwaukee, WI 53201, USA
    Dent Mater 24:221-7. 2008
    ..Nickel-titanium orthodontic wires have various temperature-dependent phases. The purpose of this study was to investigate temperature-dependent corrosion characteristics of shape memory, superelastic, and nonsuperelastic orthodontic wires...
  98. ncbi Calorimetric unfolding of the bimolecular and i-motif complexes of the human telomere complementary strand, d(C(3)TA(2))(4)
    Mahima Kaushik
    Department of Pharmaceutical Sciences, University of Nebraska Medical Center, 986025 Nebraska Medical Center, Omaha, NE 68198 6025, USA
    Biophys Chem 126:154-64. 2007
    ..Furthermore, the transition temperature, T(M), of the lower transition depends on strand concentration, while the T(M) of the higher transition is ..
  99. ncbi Interaction of cAMP receptor protein from Escherichia coli with cAMP and DNA studied by differential scanning calorimetry
    Urszula Błaszczyk
    Department of Physical Biochemistry, Faculty of Biotechnology, Jagiellonian University, Krakow, Poland
    J Protein Chem 22:285-93. 2003
    ..The thermal transition of the protein in CRP-DNA complexes, measured by CD, indicates that the protein stability in the complex is also DNA sequence-dependent...
  100. ncbi The effect of water on the glass transition of human hair
    F J Wortmann
    DWI at RWTH Aachen University, Pauwelsstrasse 8, D 52074 Aachen, Germany
    Biopolymers 81:371-5. 2006
    ..relationship between the data is suitably described by the Fox equation, yielding for human hair a glass transition temperature of T(g) = 144 degrees C, which is substantially lower than that for wool (174 degrees C)...
  101. ncbi Interfacial effects on vitrification of confined glass-forming liquids
    Olga Trofymluk
    Thermochemistry Facility and Nanomaterials in the Environment, Agriculture and Technology Organized Research Unit, University of California at Davis, Davis, CA 95616, USA
    J Chem Phys 123:194509. 2005
    ..In agreement with previous studies, where a controlled porous glass is used as a solid matrix, the glass transition temperature for o-terphenyl diminishes with decreasing pore size...

Research Grants59

  1. High-Tc susceptometer to monitor transfusional iron overload (NSR device)
    Gary M Brittenham; Fiscal Year: 2012
    ..abstract_text> ..
  2. Protease Operated Depot for Delivery of GLP-1
    Ashutosh Chilkoti; Fiscal Year: 2013
    ..The specific aims are: (1) to synthesize a library of PODs with varied ELP MW and transition temperature, number of GLP-1 repeats per oligomer, and different protease recognition sequence between the GLP-1 ..
  3. Image-guided Ultrasound-triggered Tumor Therapy
    Alexander L Klibanov; Fiscal Year: 2011
    ..Evaluate release efficacy as a function of liposome/particle membrane composition, structure, phase transition temperature and ultrasound intensity, frequency and pulse characteristics to achieve optimal release. 2...
  4. Novel Polymeric nanoparticles for drug delivery applications
    Anthony J McGoron; Fiscal Year: 2013
    ..Specific aims: (1) Measure the physico-chemical properties (FTIR, glass transition temperature, MW, NMR and degradation) of the PGMD polymer and prepare Nps and characterize their size, shape and ..
  5. A Thermo-responsive Biopharmaceutical to Enhance the Tear Production of Lacritin
    Sandeep Samudre; Fiscal Year: 2013
    ..ELPs with a transition temperature greater than 37[unreadable]C should clear quickly from the eye;however, ELPs with transition temperature ..
  6. Antimicrobial catheter
    LUIZ BARROCA DA SILVA; Fiscal Year: 2011
    ..use of a thermochromic material which can quickly change it's absorption properties at a preselected transition temperature allows for accurate control of the peak temperature...
  7. Light-Triggered Drug Release in Primed Pancreatic Tumors
    Jonathan F Lovell; Fiscal Year: 2013
    ..The release mechanism, which involves doping porphyrin bilayers with high transition temperature lipids, is based on a novel nanoscale heating phenomenon unique to porphysomes...
  8. EFFECTS OF GAMMA RADIATION ON BIOMEDICAL POLYMERS
    Cato Laurencin; Fiscal Year: 1990
    ....
  9. BASIC AND APPLIED RESEARCH ON SPERM CRYOPRESERVATION
    James Overstreet; Fiscal Year: 1992
    ..and the hypothesis that these transitions are a source of damage during CP will be tested by comparing transition temperature (Tm) for sperm that survive CP this the Tm of sperm that do not...
  10. GLASS TRANSITION TEMPERATURE AND WEAR OF DENTAL RESINS
    JAN STANNARD; Fiscal Year: 1993
    ..The hypothesis of this proposal is that the wear of composites are related to the glass transition temperature (Tg) of the polymer(s) used in composites; specifically wear is hypothesized to be lowest when the polymer'..
  11. Development of Chemical Receptors for Proteins
    Sanku Mallik; Fiscal Year: 2005
    ..After fabrication, these liposomes (in the unpolymerized state, above the gel-transition temperature) will be allowed to interact with the protein...
  12. Internal Polymeric Vessel Occluder for Temporary Vascular Occlusion of the Kidney
    PETER MADRAS; Fiscal Year: 2007
    ..However, the polymer used in these preliminary experiments has a lower than optimal transition temperature for a solid organ...
  13. Molecularly filled composites for improved dental restoratives
    KATHLEEN SCHRECK; Fiscal Year: 2010
    ..Properties of the resulting composites, including glass transition temperature, modulus, shrinkage, and mechanical strength will be measured in order to determine their ability to serve ..
  14. High Tc susceptometer for magnetic measure of body iron
    Gary Brittenham; Fiscal Year: 2005
    ..S. and worldwide. ..
  15. WATER SORPTION OF GLASSY DENTAL POLYMERS
    Robert Kusy; Fiscal Year: 1990
    ..This aim includes determination of the glass transition temperature, Tg, above which a polymer loses rigidity...
  16. Swellable Templated Copolymers
    RUDOLF SEITZ; Fiscal Year: 2006
    ..This polymer undergoes a thermal phase transition, coming out of solution above the thermal phase transition temperature. Molecular recognition occurs above the thermal phase transition...
  17. EFFECTS OF PRESSURE ON MEMBRANE-COMPONENT INTERACTIONS
    Suzanne Scarlata; Fiscal Year: 1992
    ..of charged vesicles under pressure will be established by monitoring the gel to liquid crystal phase transition temperature with fluorescent probes...
  18. LIPID PHASE TRANSITIONS AND PLATELET ACTIVATION
    Fern Tablin; Fiscal Year: 2001
    ..Recent work shows that AFGPs appear to stabilize platelets during chilling and storage below the phase transition temperature. The proposal seeks to better define the mechanisms involved in the cold storage lesion in platelets ..
  19. TOXICITY OF CHLOROPHENOLS IN MITOCHONDRIAL MEMBRANES
    Pavel Smejtek; Fiscal Year: 1990
    ..associated with transmembrane translocation of protons, (f) determination of changes of the gel- to-fluid transition temperature of lipid membranes as a measure of chlorophenol-induced changes of interlipid interaction, and exploration ..
  20. HYPERTHERMIA AND PERFUSION EFFECTS IN CANCER THERAPY
    Mark Dewhirst; Fiscal Year: 2009
    ..with novel thermolabile liposomes that release contents within seconds of reaching their phase transition temperature, (3) conduct phase III trials comparing best conventional therapy q HT, (4) examine whether ..
  21. Image-guided Ultrasound-triggered Tumor Therapy
    Alexander Klibanov; Fiscal Year: 2007
    ..Evaluate release efficacy as a function of liposome/particle membrane composition, structure, phase transition temperature and ultrasound intensity, frequency and pulse characteristics to achieve optimal release. 2...
  22. Nano-aggregates Improve Contraction Stress & Toughness in Dental Composite Resins
    Valerie Lee; Fiscal Year: 2009
    ..Plasticizers reduce the glass transition temperature (Tg) during curing such that chain mobility remains high for a longer time during the course of the ..
  23. ANTIATHEROGENIC ACTIONS OF OMEGA-3 FATTY ACIDS
    Papasani Subbaiah; Fiscal Year: 1990
    ..changes we have already shown in lipoproteins modify their physicochemical characteristics (size, phase transition temperature, subpopulation distribution, fluidity), and functional properties (the LDL catabolic pathways, HDL's ..
  24. MEMBRANE SURFACE PRESSURE--MEASUREMENT AND SIGNIFICANCE
    Robert MacDonald; Fiscal Year: 1992
    ..Goal I will be to vary the surface pressure until monolayer and bilayer correspond with respect to phase transition temperature, 2-dimensional diffusion coefficient and partition coefficient of a hydrophobic probe...
  25. Novel Controlled Delivery Systems for Contraceptives
    Jagdish Singh; Fiscal Year: 2005
    ..temperature sensitive triblock copolymers and characterize them for critical gel concentration, gel transition temperature (i.e...
  26. CELLULAR THERMOSTABILITY AND THERMOTOLERANCE
    JAMES LEPOCK; Fiscal Year: 2001
    ..determine the identity of the thermolabile proteins of the nuclear matrix and nuclei that denature at the transition temperature (Tm =46 C for V79 cells and 47.4 C for human A549 cells) of the critical target by thermal gel analysis...
  27. FEASIBILTITY OF PRECLINICAL SCALEUP OF LIPOSOMAL TNF
    ALAN HAYMAN; Fiscal Year: 1992
    ..composed of DPPC, DSPC, and DSPE-PEG will be defined based on high binding to acylated TNF, high phase-transition temperature, and stability to storage under mock physiological conditions...
  28. IMPROVED PREVENTIVE/RESTORATIVE RESINS AND COMPOSITES
    SABINE DICKENS; Fiscal Year: 2006
    ..Fracture toughness, conversion, water sorption and the viscoelastic properties and glass transition temperature of the polymers will be determined...
  29. X-RAY DIFFRACTION OF NORMAL AND PATHOLOGICAL MEMBRANES
    GEORGE BRADY; Fiscal Year: 1980
    ..The work will be done on suspensions of membrane vesicles below and above the lipid transition temperature, when applicable, and the emphasis will be on the determination of the protein distribution and of the ..
  30. Cornea Preservation by Vitrification
    GREGORY FAHY; Fiscal Year: 2004
    ..In phase III we will launch commercial products and services. ..
  31. Improved Renal Preservation
    GREGORY FAHY; Fiscal Year: 2003
    ..The ability to preserve kidneys with less damage prior to transplantation will significantly reduce the costs and risks of post-transplant dialysis, reduce rejection episodes, and increase the net organ supply. ..
  32. Extended Cardiac Preservation
    GREGORY FAHY; Fiscal Year: 2005
    ..Should that be the case, the combination product, URS with an intermittent perfusion system, will be tested with greater priority. ..
  33. Regulation of Kv3.1 by MiRPs in Auditory Neurons
    GEOFFREY ABBOTT; Fiscal Year: 2006
    ..1 alpha subunits. 3. Examine the predicted effects in auditory neurons of MiRP modulation of Kv3.1, Kv3.3 and Kv3.1-Kv3.3 channels using a combination of heterologous coexpression experiments and computer simulations. ..
  34. Analysis of ATP7B in Screening for Wilson Disease
    Steven Dobrowolski; Fiscal Year: 2004
    ..Prospective screening for WD improves efficacy of treatment and quality of life for affected patients. Genotyping is the best option to confirm results based upon ceruloplasmin analysis in asymptomatic patients. ..
  35. LCAT AND PATHOPHYSIOLOGY OF PHOSPHOLIPID SPECIES
    Papasani Subbaiah; Fiscal Year: 2002
    ..These studies should provide novel insights into the physiological importance of LCAT beyond its role in cholesterol esterification. ..
  36. ROLE OF SPHINGOMYELIN IN LIPOPROTEIN METABOLISM
    Papasani Subbaiah; Fiscal Year: 2008
    ..These studies will provide novel insights into the physiological role of this important phospholipid, and could possibly open up novel therapeutic targets against inflammation and atherosclerosis. ..
  37. A Novel Inhibitor of Stat3 for Prostate Cancer Therapy
    Naijie Jing; Fiscal Year: 2009
    ..abstract_text> ..
  38. Distribution of PCB Atropisomers
    Hans Joachim Lehmler; Fiscal Year: 2008
    ..The long term career goal is to establish Dr. Lehmler as an investigator with independent research status and funding. ..
  39. Micro Lock-In Amplifer for Cortisol and Catecholamines
    Antonio Garcia; Fiscal Year: 2003
    ..Applications to wellness, therapeutic drug monitoring, and bio or chemical exposure are envisioned using the general approach of microlock-in amplifier detection technology. ..
  40. FilmArray-A Closed System for Multi-Pathogen Screening
    Steven Dobrowolski; Fiscal Year: 2005
    ..Pouches are designed to assay for candidate infectious agents consistent with clinical presentation. ..
  41. MGB Eclipse Probe Detection of Category A Organisms
    Irina Afonina; Fiscal Year: 2003
    ..In Phase II, we will expand the number of Category A organisms and improve throughput by multiplexing. In addition, commercial prototype MGB Eclipse(TM) probe assays will be developed for each of these organisms. ..
  42. Novel MRI Contrast Agent for Monitoring Thermal Ablation
    Nathan Mcdannold; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  43. Modular design of central metabolism in methylotrophs
    Gregory Crowther; Fiscal Year: 2006
    ..abstract_text> ..
  44. CYROBIOLOGICAL PRESERVATION OF ANOPHELES EMBRYOS
    Peter Mazur; Fiscal Year: 2001
    ..Such a mutant has been isolated in a related species, Anopheles quadrimaculatus. We also propose to collaborate with the Laboratory of Parasitic Diseases at NIH in the cryopreservation of A gambiae larvae. ..
  45. Genotype Analysis for Diagnosis of Urea Cycle Disorders
    Steven Dobrowolski; Fiscal Year: 2007
    ..The proposed assay panels provide a means to readily assess gene that are well established to contribute to hyperammonemia. [unreadable] [unreadable] [unreadable]..
  46. Contrast Enhanced Ultrasound with Therapeutic Modality
    Margaret Wheatley; Fiscal Year: 2004
    ..These studies are a prelude to show feasibility prior to in vivo studies. ..
  47. Thermo mechanical stress in cryopreserved blood vessels
    Yoed Rabin; Fiscal Year: 2006
    ..Although the experimental work in this study is targeted to blood vessels, the results of this study could be expanded and become useful for a wide variety of cryopreserved natural tissues and engineered constructs. ..
  48. Controlled Delivery System for Naltrexone
    EMMANUEL AKALA; Fiscal Year: 2004
    ....
  49. SPECTROSCOPIC AND RELATED STUDIES ON LENS MEMBRANE LIPID
    Douglas Borchman; Fiscal Year: 2003
    ..An understanding of these alterations will facilitate the development of therapies to prevent or perhaps even reverse these changes. ..
  50. Development and Characterization of a Novel Contrast Agent
    Margaret Wheatley; Fiscal Year: 2009
    ..A diagnostic tool that could safely and non-invasively detect tumors at an early stage, before metastasis, would be a great advance in health care. ..
  51. Perfluorocarbon Materials As Drug Delivery Vehicles
    Hans Joachim Lehmler; Fiscal Year: 2004
    ..abstract_text> ..
  52. Developing computerized tools for cryosurgery planning
    Yoed Rabin; Fiscal Year: 2007
    ..Such visualization capabilities will provide surgeons with insights into the physics of cryosurgery that are difficult to obtain from physical experiments or surgical practice. [unreadable] [unreadable]..
  53. Purchase of a 500 MHz Solid State NMR Spectrometer
    Ayyalusamy Ramamoorthy; Fiscal Year: 2007
    ..unreadable] [unreadable] All the major users of the instrument are supported by NIH R01 grants. [unreadable] [unreadable] [unreadable]..
  54. High Toughness Textured Ceramics for Biomedical Use
    Isabelle Denry; Fiscal Year: 2007
    ..Potential applications include dental restorations, dental implant superstructures, and bone replacement implants. ..
  55. DEVELOPING A POTENT INHIBITOR OF HIV-I INTEGRASE
    Naijie Jing; Fiscal Year: 2001
    ....
  56. Multidisciplinary Training Program in Human Imaging
    GEOFFREY CLARKE; Fiscal Year: 2008
    ..abstract_text> ..
  57. Structure and function of antimicrobial peptides
    Ayyalusamy Ramamoorthy; Fiscal Year: 2008
    ..These peptides have therapeutic potential as antibiotics and have particular importance for cystic fibrosis patients. ..
  58. Newborn screening for PKU and BH4 responsiveness
    Steven Dobrowolski; Fiscal Year: 2008
    ..High resolution melt profiling will play a role to identify BH4 responsive PKU patients. ..
  59. ACS Symposium, Frontiers in Chemical Toxicology
    Michael Stone; Fiscal Year: 2008
    ..Highly respected scientists from a variety of disciplines have agreed to present their most recent findings. [unreadable] [unreadable] [unreadable] [unreadable]..