Genomes and Genes
Summary: The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
Publications524 found, 100 shown here
- Stochastic gene expression in a single cellMichael B Elowitz
Laboratory of Cancer Biology, Center for Studies in Physics and Biology, Rockefeller University, New York, NY 10021, USA
Science 297:1183-6. 2002..These results establish a quantitative foundation for modeling noise in genetic networks and reveal how low intracellular copy numbers of molecules can fundamentally limit the precision of gene regulation...
- Shedding light onto live molecular targetsRalph Weissleder
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts, USA
Nat Med 9:123-8. 2003
- Fluorescence imaging with near-infrared light: new technological advances that enable in vivo molecular imagingVasilis Ntziachristos
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th Street 5406, Charlestown MA 02129 2060, USA
Eur Radiol 13:195-208. 2003..development in biomedical imaging is the non-invasive mapping of molecular events in intact tissues using fluorescence. Underpinning to this development is the discovery of bio-compatible, specific fluorescent probes and proteins ..
- A photoactivatable GFP for selective photolabeling of proteins and cellsGeorge H Patterson
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Science 297:1873-7. 2002..victoria green fluorescent protein (GFP) that, after intense irradiation with 413-nanometer light, increases fluorescence 100 times when excited by 488-nanometer light and remains stable for days under aerobic conditions...
- LNA flow-FISH: a flow cytometry-fluorescence in situ hybridization method to detect messenger RNA using locked nucleic acid probesKelly L Robertson
Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Washington, DC 20375, USA
Anal Biochem 390:109-14. 2009We present a novel method using flow cytometry-fluorescence in situ hybridization (flow-FISH) to detect specific messenger RNA (mRNA) in suspended cells using locked nucleic acid (LNA)-modified oligonucleotide probes...
- In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epitheliaMelissa C Skala
Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA
Proc Natl Acad Sci U S A 104:19494-9. 2007..Increased intracellular variability in the redox ratio, NADH, and FAD fluorescence lifetimes were observed in precancerous cells compared with normal cells.
- Real-time fluorescence loop mediated isothermal amplification for the diagnosis of malariaNaomi W Lucchi
Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America
PLoS ONE 5:e13733. 2010..we attempted to improve this method for malaria diagnosis by using a simple and portable device capable of performing both the amplification and detection (by fluorescence) of LAMP in one platform. We refer to this as the RealAmp method.
- A new in vivo model to test anti-tuberculosis drugs using fluorescence imagingAndrea Zelmer
Immunology and Infection Department, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel St, London WC1E 7HT, UK
J Antimicrob Chemother 67:1948-60. 2012..Our aim was to develop a more efficient, fluorescence-based imaging assay to test new antibiotics in a mouse model using Mtb reporter strains.
- Printing proteins as microarrays for high-throughput function determinationG MacBeath
Center for Genomics Research, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
Science 289:1760-3. 2000..Three applications for protein microarrays were demonstrated: screening for protein-protein interactions, identifying the substrates of protein kinases, and identifying the protein targets of small molecules...
- Measurement of optical trapping forces by use of the two-photon-excited fluorescence of microspheresA V Kachynski
The Institute for Lasers, Photonics, and Biophotonics, State University of New York at Buffalo, 458 Natural Sciences Complex, Buffalo, New York 14260 3000, USA
Opt Lett 28:2288-90. 2003A novel technique for the calibration of laser trapping systems that utilizes two-photon-excited fluorescence of commercial dye-stained microspheres has been demonstrated...
- Quantum dots for live cells, in vivo imaging, and diagnosticsX Michalet
Department of Chemistry and Biochemistry, University of California, 607 Charles E Young Drive East, Los Angeles, CA 90095, USA
Science 307:538-44. 2005....
- Single-molecule fluorescence reveals sequence-specific misfolding in multidomain proteinsMadeleine B Borgia
University of Cambridge Chemical Laboratory, Lensfield Road, Cambridge CB2 1EW, UK
Nature 474:662-5. 2011..Diversifying the sequence between neighbouring domains seems to be a successful evolutionary strategy to avoid misfolding in multidomain proteins...
- Fluorescence-guided surgical sampling of glioblastoma identifies phenotypically distinct tumour-initiating cell populations in the tumour mass and marginS G M Piccirillo
Department of Clinical Neurosciences, Cambridge Centre for Brain Repair, University of Cambridge, Forvie Site, Robinson Way, Cambridge CB2 0PY, UK
Br J Cancer 107:462-8. 2012..We tested the use of 5-aminolevulinic acid (5-ALA) fluorescence to objective tissue sampling and to derive tumour-initiating cells (TICs) from mass and margin.
- Near-infrared fluorescence: application to in vivo molecular imagingScott A Hilderbrand
Center for Systems Biology, Massachusetts General Hospital Harvard Medical School, Simches Research Center, 185 Cambridge St, Suite 5 210, Boston, MA 02114, USA
Curr Opin Chem Biol 14:71-9. 2010..The use of optical probes with near-infrared fluorescence allows for improved photon penetration through tissue and minimizes the effects of tissue autofluorescence...
- Identification of a slowly inducible zeaxanthin-dependent component of non-photochemical quenching of chlorophyll fluorescence generated under steady-state conditions in ArabidopsisManuela Nilkens
Biochemie der Pflanzen, Heinrich Heine Universitat Dusseldorf, Dusseldorf, Germany
Biochim Biophys Acta 1797:466-75. 2010..Comparative analysis of different NPQ mutants from Arabidopsis showed that qZ was clearly not related to qE, qT or qI and thus represents a separate, Zx-dependent NPQ component...
- Near-infrared fluorescence (NIRF) imaging in breast-conserving surgery: assessing intraoperative techniques in tissue-simulating breast phantomsR G Pleijhuis
Department of Surgery, University Medical Center Groningen, Hanzeplein 1, Groningen, The Netherlands
Eur J Surg Oncol 37:32-9. 2011..In this study, the potential of near-infrared fluorescence (NIRF) imaging in BCS for pre- and intraoperative tumour localization, margin status assessment and detection ..
- PVP-coated graphene oxide for selective determination of ochratoxin A via quenching fluorescence of free aptamerLinfeng Sheng
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China
Biosens Bioelectron 26:3494-9. 2011..by Aspergillus Ochraceus and Penicillium verrucosumm, utilizing graphene oxide as quencher which can quench the fluorescence of FAM (carboxyfluorescein) attached to toxin-specific aptamer...
- Multifunctional nanoprobes for upconversion fluorescence, MR and CT trimodal imagingHuaiyong Xing
State Key Laboratory of High Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, PR China
Biomaterials 33:1079-89. 2012Early diagnosis probes that combine fluorescence, X-ray computed tomography (CT) and magnetic resonance (MR) imagings are anticipated to give three dimensional (3D) details of tissues and cells of high resolution and sensitivity...
- Instrumentation in developing chlorophyll fluorescence biosensing: a reviewArturo A Fernandez-Jaramillo
Biosystems Engineering CA, Postgraduate Study Division, Engineering Faculty, Autonomous University of Queretaro, Cerro de las Campanas St, Querétaro 76010, Qro Mexico
Sensors (Basel) 12:11853-69. 2012Chlorophyll fluorescence can be defined as the red and far-red light emitted by photosynthetic tissue when it is excited by a light source...
- The FLARE intraoperative near-infrared fluorescence imaging system: a first-in-human clinical trial in breast cancer sentinel lymph node mappingSusan L Troyan
Breast Care Center, Department of Surgery, Beth Israel Deaconess Medical Center, Boston, MA, USA
Ann Surg Oncol 16:2943-52. 2009..The FLARE imaging system overcomes this major obstacle...
- Fluorescence nanoparticles "quantum dots" as drug delivery system and their toxicity: a reviewShirin Ghaderi
Centre for Nanotechnology and Regenerative Medicine, Division of Surgery and Interventional Science, University College London, UK
J Drug Target 19:475-86. 2011b>Fluorescence nanocrystals or quantum dots (QDs) are engineered nanoparticles (NP) that have shown great promise with potential for many biological and biomedical applications, especially in drug delivery/activation and cellular imaging...
- Fluorescence and atomic force microscopy imaging of wall teichoic acids in Lactobacillus plantarumGuillaume Andre
Institute of Condensed Matter and Nanosciences Bio and Soft Matter, Université catholique de Louvain, Belgium
ACS Chem Biol 6:366-76. 2011..Here, we used single-molecule atomic force microscopy (AFM) combined with fluorescence microscopy to image the distribution of wall teichoic acids (WTAs) in Lactobacillus plantarum, in relation with ..
- Randomized, double-blind comparison of indocyanine green with or without albumin premixing for near-infrared fluorescence imaging of sentinel lymph nodes in breast cancer patientsMerlijn Hutteman
Department of Surgery, Leiden University Medical Center, Albinusdreef 2, 2300 RC Leiden, The Netherlands
Breast Cancer Res Treat 127:163-70. 2011Near-infrared (NIR) fluorescence imaging has the potential to improve sentinel lymph node (SLN) mapping in breast cancer. Indocyanine green (ICG) is currently the only clinically available fluorophore that can be used for SLN mapping...
- Cerenkov emission induced by external beam radiation stimulates molecular fluorescenceJohan Axelsson
Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
Med Phys 38:4127-32. 2011..This study quantifies the emissions and also demonstrates that the Cerenkov emission can excite a fluorophore, protoporphyrin IX (PpIX), embedded in biological phantoms...
- Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementationChang Deng Hu
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA
Mol Cell 9:789-98. 2002Networks of protein interactions coordinate cellular functions. We describe a bimolecular fluorescence complementation (BiFC) assay for determination of the locations of protein interactions in living cells...
- A fluorescence-based assay for N-myristoyltransferase activityVictor Goncalves
Department of Chemistry, Imperial College London, London SW7 2AZ, UK
Anal Biochem 421:342-4. 2012..Here we report a sensitive fluorescence-based assay to study the enzymatic activity of human NMT1 and NMT2 based on detection of CoA by 7-diethylamino-..
- Simple non-invasive assessment of advanced glycation endproduct accumulationR Meerwaldt
Division of Vascular Medicine, Department of Medicine U3 129, University Hospital Groningen, Hanzeplein, 9700 RB, Groningen, The Netherlands
Diabetologia 47:1324-30. 2004..We validated its use by comparing the values obtained using the AFR with the AGE content measured in extracts from skin biopsies of diabetic and control subjects...
- Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imagingHui Wang Ai
Department of Chemistry, University of Alberta, Edmonton, AB, Canada T6G 2G2
Biochem J 400:531-40. 2006..The new mTFP1 is one of the brightest and most photostable FPs reported to date. In addition, the fluorescence is insensitive to physiologically relevant pH changes and the fluorescence lifetime decay is best fitted as a ..
- Skin autofluorescence is a strong predictor of cardiac mortality in diabetesRobbert Meerwaldt
Department of Medicine, University Medical Center Groningen, Groningen, Netherlands
Diabetes Care 30:107-12. 2007..The aim of the present study was to evaluate the relationship between skin autofluorescence and metabolic burden (hyperglycemia and hyperlipidemia) and its relationship with CHD and mortality...
- Bacterial genetics by flow cytometry: rapid isolation of Salmonella typhimurium acid-inducible promoters by differential fluorescence inductionR H Valdivia
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305, USA
Mol Microbiol 22:367-78. 1996..in response to the phagosomal environment, we have applied a novel enrichment strategy, termed differential fluorescence induction (DFI), to screen an S. typhimurium library for promoters that are upregulated at pH 4.5...
- Mechanisms of tryptophan fluorescence shifts in proteinsJ T Vivian
Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA
Biophys J 80:2093-109. 2001Tryptophan fluorescence wavelength is widely used as a tool to monitor changes in proteins and to make inferences regarding local structure and dynamics...
- Membrane lipids of symbiotic algae are diagnostic of sensitivity to thermal bleaching in coralsDan Tchernov
Environmental Biophysics and Molecular Ecology Program, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 71 Dudley Road, New Brunswick, NJ 08901, USA
Proc Natl Acad Sci U S A 101:13531-5. 2004..Measurements of variable chlorophyll fluorescence kinetic transients indicate that thermally damaged membranes are energetically uncoupled but remain capable of ..
- mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structuresSimone Kredel
Institute of General Zoology and Endocrinology, University of Ulm, Ulm, Germany
PLoS ONE 4:e4391. 2009..The unique spectral properties make mRuby the marker of choice for a multitude of cell biological applications. Moreover, the use of mRuby has allowed novel insights in the biology of organelles responsible for severe human diseases...
- Pre-clinical whole-body fluorescence imaging: Review of instruments, methods and applicationsFrederic Leblond
Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
J Photochem Photobiol B 98:77-94. 2010b>Fluorescence sampling of cellular function is widely used in all aspects of biology, allowing the visualization of cellular and sub-cellular biological processes with spatial resolutions in the range from nanometers up to centimeters...
- Imaging intracellular fluorescent proteins at nanometer resolutionEric Betzig
Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA
Science 313:1642-5. 2006....
- Fluorescence-guided resection of glioblastoma multiforme by using 5-aminolevulinic acid-induced porphyrins: a prospective study in 52 consecutive patientsW Stummer
Department of Neurosurgery, Institute for Neuropathology, Klinikum Grosshadern, Ludwig Maximilians University, Munich, Germany
J Neurosurg 93:1003-13. 2000..In this study the authors analyze the influence of fluorescence-guided resection on postoperative magnetic resonance (MR) imaging and survival in a series of patients who ..
- Sodium octanoate to reverse indoxyl sulfate and p-cresyl sulfate albumin binding in uremic and normal serum during sample preparation followed by fluorescence liquid chromatographyHenriette de Loor
Department of Medicine, University Hospital Gasthuisberg, Leuven, Belgium
J Chromatogr A 1216:4684-8. 2009..Sodium octanoate binding competition is a novel sample preparation that allows for direct quantification of indoxyl sulfate and p-cresyl sulfate...
- Ratiometric fluorescence imaging of cellular glutathioneGun Joong Kim
Department of Chemistry, Hankuk University of Foreign Studies, Yongin, Republic of Korea
Org Lett 13:2799-801. 2011A fluorescent probe (1) with a hydrogen bond was designed for the detection of GSH. The probe exhibited a rapid and ratiometric fluorescence response to GSH through a Michael reaction and allowed us to obtain clear cellular images for GSH.
- Fluorescence imaging of cellular metabolites with RNAJeremy S Paige
Department of Pharmacology, Weill Medical College, Cornell University, New York, NY 10065, USA
Science 335:1194. 2012..an approach for generating fluorescent sensors based on Spinach, an RNA sequence that binds and activates the fluorescence of a small-molecule fluorophore...
- Thermodynamics of the multi-stage DNA lesion recognition and repair by formamidopyrimidine-DNA glycosylase using pyrrolocytosine fluorescence--stopped-flow pre-steady-state kineticsNikita A Kuznetsov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, 630090, Russia
Nucleic Acids Res 40:7384-92. 2012....
- Chlorophyll fluorescence analysis: a guide to good practice and understanding some new applicationsE H Murchie
Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Nottingham, Sutton Bonington LE12 5RD, UK
J Exp Bot 64:3983-98. 2013Chlorophyll fluorescence is a non-invasive measurement of photosystem II (PSII) activity and is a commonly used technique in plant physiology...
- Superoxide reacts with hydroethidine but forms a fluorescent product that is distinctly different from ethidium: potential implications in intracellular fluorescence detection of superoxideHongtao Zhao
Biophysics Research Institute and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
Free Radic Biol Med 34:1359-68. 2003..formation of a two-electron oxidized product, ethidium (E+), which binds to DNA and leads to the enhancement of fluorescence (excitation, 500-530 nm; emission, 590-620 nm)...
- Self-illuminating quantum dot conjugates for in vivo imagingMin Kyung So
Molecular Imaging Program, Department of Radiology, Stanford University, 1201 Welch Road, Stanford, California 94305 5484, USA
Nat Biotechnol 24:339-43. 2006..Compared with existing quantum dots, self-illuminating quantum dot conjugates have greatly enhanced sensitivity in small animal imaging, with an in vivo signal-to-background ratio of > 10(3) for 5 pmol of conjugate...
- Visualization of dynamics of plant-pathogen interaction by novel combination of chlorophyll fluorescence imaging and statistical analysis: differential effects of virulent and avirulent strains of P. syringae and of oxylipins on A. thalianaSusanne Berger
Julius von Sachs Institute of Biosciences, Department of Pharmaceutical Biology, Julius von Sachs Platz 2, D 97082 Wurzburg, Germany
J Exp Bot 58:797-806. 2007..12-oxo-phytodienoic acid (OPDA) and jasmonic acid on photosynthesis was investigated by kinetic chlorophyll fluorescence imaging and compared with the effects of infection by virulent and avirulent strains of Pseudomonas syringae...
- Single-cell quantification of molecules and rates using open-source microscope-based cytometryAndrew Gordon
The Molecular Sciences Institute, 2168 Shattuck Avenue, Berkeley, California 94704, USA
Nat Methods 4:175-81. 2007..Owing to their sensitivity, accuracy and ability to track changes in individual cells over time, these microscope methods may complement flow-cytometric measurements for studies of the quantitative physiology of cellular systems...
- Magnetic resonance-coupled fluorescence tomography scanner for molecular imaging of tissueScott C Davis
Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
Rev Sci Instrum 79:064302. 2008..The design allows spectral fitting of the remission light, thereby separating the fluorescence signal from the nonspecific background, which improves the accuracy and sensitivity when imaging low ..
- Far-red fluorescence: a direct spectroscopic marker for LHCII oligomer formation in non-photochemical quenchingYuliya Miloslavina
Max Planck Institut fur Bioanorganische Chemie, Germany
FEBS Lett 582:3625-31. 2008Time-resolved fluorescence on oligomers of the main light-harvesting complex from higher plants indicate that in vitro oligomerization leads to the formation of a weakly coupled inter-trimer chlorophyll-chlorophyll (Chl) exciton state ..
- Delayed fluorescence in photosynthesisVasilij Goltsev
Department of Biophysics and Radiobiology, Faculty of Biology, St Kliment Ohridski University of Sofia, 8 Dragan Tzankov Boulevard, Sofia, Bulgaria
Photosynth Res 101:217-32. 2009..occurs before the utilization of the excitation energy in the primary photochemical reaction is called prompt fluorescence. Light emission can also be observed from repopulated excited chlorophylls as a result of recombination of the ..
- Double labeling of oligonucleotide probes for fluorescence in situ hybridization (DOPE-FISH) improves signal intensity and increases rRNA accessibilityKilian Stoecker
Department of Microbial Ecology, University of Vienna, Vienna, Austria
Appl Environ Microbiol 76:922-6. 2010b>Fluorescence in situ hybridization (FISH) with singly labeled rRNA-targeted oligonucleotide probes is widely applied for direct identification of microbes in the environment or in clinical specimens...
- Simultaneous in vivo recording of prompt and delayed fluorescence and 820-nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensisReto J Strasser
Weed Research Laboratory, Nanjing Agricultural University, Nanjing 210095, China
Biochim Biophys Acta 1797:1313-26. 2010A new instrument (M-PEA), which measures simultaneously kinetics of prompt fluorescence (PF), delayed fluorescence (DF) and modulated light reflection at 820nm (MR), was used to screen dark-adapted leaves of the resurrection plant ..
- 5-aminolevulinic acid (5-ALA)-induced fluorescence in intracerebral metastases: a retrospective studyMarcel A Kamp
Neurochirurgische Klinik, Heinrich Heine Universitat Dusseldorf, Moorenstraße 5, Geb 13 71, 40225 Dusseldorf, Germany
Acta Neurochir (Wien) 154:223-8; discussion 228. 2012..Microsurgical, circumferential stripping of intracerebral metastases often proves to be insufficient to prevent local tumor recurrence...
- Visualization of cofilin-actin and Ras-Raf interactions by bimolecular fluorescence complementation assays using a new pair of split Venus fragmentsKazumasa Ohashi
Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan
Biotechniques 52:45-50. 2012The bimolecular fluorescence complementation (BiFC) assay is a method for visualizing protein-protein interactions in living cells...
- Fluorescence detection of DNA, adenosine-5'-triphosphate (ATP), and telomerase activity by zinc(II)-protoporphyrin IX/G-quadruplex labelsZhanxia Zhang
Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Anal Chem 84:4789-97. 2012The zinc(II)-protoporphyrin IX (ZnPPIX) fluorophore binds to G-quadruplexes, and this results in the enhanced fluorescence of the fluorophore...
- FMT-XCT: in vivo animal studies with hybrid fluorescence molecular tomography-X-ray computed tomographyAngelique Ale
Technische Universität München and Helmholtz Zentrum München, Institute for Biological and Medical Imaging, Neuherberg, Germany
Nat Methods 9:615-20. 2012..Here we examined in vivo performance of a camera-based hybrid fluorescence molecular tomography (FMT) system for 360° imaging combined with X-ray computed tomography (XCT)...
- Fluorescence-based reporter for gauging cyclic di-GMP levels in Pseudomonas aeruginosaMorten T Rybtke
Department of International Health, Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Appl Environ Microbiol 78:5060-9. 2012..aeruginosa...
- Fluorescence enhancement at docking sites of DNA-directed self-assembled nanoantennasG P Acuna
NanoBioSciences Group, Institute for Physical and Theoretical Chemistry, Technische Universitat Braunschweig, Hans Sommer Strasse 10, 38106 Braunschweig, Germany
Science 338:506-10. 2012We introduce self-assembled nanoantennas to enhance the fluorescence intensity in a plasmonic hotspot of zeptoliter volume...
- The origin of fluorescence from graphene oxideJingzhi Shang
Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371
Sci Rep 2:792. 2012Time-resolved fluorescence measurements of graphene oxide in water show multiexponential decay kinetics ranging from 1 ps to 2 ns...
- A label-free, quantitative assay of amyloid fibril growth based on intrinsic fluorescenceDorothea Pinotsi
Department of Chemical Engineering and Biotechnology, University of Cambridge, Pembroke Street, Cambridge, CB2 3RA, UK
Chembiochem 14:846-50. 2013Kinetic assay of seeded growth: The graph shows the variation in intrinsic fluorescence intensity of amyloid fibrils...
- Validation and development of fluorescence two-dimensional differential gel electrophoresis proteomics technologyR Tonge
Proteomics Group, Enabling Science and Technology Biology, CTL, AstraZeneca Pharmaceuticals, Alderley Park, Macclesfield, Cheshire, SK10 7TG, UK
Proteomics 1:377-96. 2001b>Fluorescence two-dimensional differential gel electrophoresis (2-D DIGE*) is a new development in protein detection for two-dimensional gels...
- Fluorescence protease protection of GFP chimeras to reveal protein topology and subcellular localizationHolger Lorenz
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bldg 18T Library Drive, Bethesda, Maryland 20892, USA
Nat Methods 3:205-10. 2006..Here we describe a new fluorescence-based technique, fluorescence protease protection (FPP), for investigating the topology of proteins and for ..
- Coregistered fluorescence-enhanced tumor resection of malignant glioma: relationships between δ-aminolevulinic acid-induced protoporphyrin IX fluorescence, magnetic resonance imaging enhancement, and neuropathological parameters. Clinical articleDavid W Roberts
Section of Neurosurgery, Dartmouth Hitchcock Medical Center, Lebanon, New Hampshire, USA
J Neurosurg 114:595-603. 2011The aim of this study was to investigate the relationships between intraoperative fluorescence, features on MR imaging, and neuropathological parameters in 11 cases of newly diagnosed glioblastoma multiforme (GBM) treated using ..
- Sentinel lymph node mapping by indocyanin green fluorescence imaging in oropharyngeal cancer - preliminary experienceMarius G Bredell
Department of Craniomaxillofacial Surgery, University Hospital of Zurich, Zurich, Switzerland
Head Neck Oncol 2:31. 2010..The aim of this paper was to evaluate the potential application of Indocyanin Green (ICG) in the mapping and detection of sentinel lymph nodes (SLN) in cancers of the head and neck...
- Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide neural expression patternsStefanie Hampel
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
Nat Methods 8:253-9. 2011..The ability to independently visualize multiple lineage or neuron projections in the same preparation greatly advances the goal of mapping how neurons connect into circuits...
- Quantitative fluorescence in intracranial tumor: implications for ALA-induced PpIX as an intraoperative biomarkerPablo A Valdes
Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire, USA
J Neurosurg 115:11-7. 2011Accurate discrimination between tumor and normal tissue is crucial for optimal tumor resection. Qualitative fluorescence of protoporphyrin IX (PpIX), synthesized endogenously following δ-aminolevulinic acid (ALA) administration, has been ..
- Coherent nonlinear optical imaging: beyond fluorescence microscopyWei Min
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Annu Rev Phys Chem 62:507-30. 2011The quest for ultrahigh detection sensitivity with spectroscopic contrasts other than fluorescence has led to various novel approaches to optical microscopy of biological systems...
- An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitchingMichele L Markwardt
Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
PLoS ONE 6:e17896. 2011..To improve the fluorescence properties of Cerulean, we used the X-ray structure to rationally target specific amino acids for optimization ..
- δ-aminolevulinic acid-induced protoporphyrin IX concentration correlates with histopathologic markers of malignancy in human gliomas: the need for quantitative fluorescence-guided resection to identify regions of increasing malignancyPablo A Valdes
Dartmouth Medical School, Dartmouth College, Hanover, New Hampshire, USA
Neuro Oncol 13:846-56. 2011..identify regions of increasing malignancy in low- and high-grade gliomas beyond the capabilities of current fluorescence imaging in patients undergoing fluorescence-guided resection (FGR)...
- Impact of the combination of 5-aminolevulinic acid-induced fluorescence with intraoperative magnetic resonance imaging-guided surgery for gliomaAtsushi Tsugu
Department of Neurosurgery, Tokai University School of Medicine, Kanagawa, Japan
World Neurosurg 76:120-7. 2011..intraoperative magnetic resonance imaging (MRI)-guided resection with resection guided by 5-aminolevulinic acid (5-ALA)-induced fluorescence in patients with gliomas and to evaluate the impact of intraoperative MRI in glioma surgery.
- Noninvasive real-time fluorescence imaging of the lymphatic uptake of BSA-IRDye 680 conjugate administered subcutaneously in miceFang Wu
Department of Pharmaceutical Sciences, University at Buffalo, State University of New York, Amherst, New York 14260, USA
J Pharm Sci 101:1744-54. 2012..goal of our studies was to determine lymphatic uptake of bovine serum albumin (BSA) using real-time noninvasive fluorescence imaging. BSA labeled with near-infrared dye (IRDye) 680 was used as a model protein-dye conjugate...
- Novel single chain cAMP sensors for receptor-induced signal propagationViacheslav O Nikolaev
Institute of Pharmacology and Toxicology, University of Wurzburg, Versbacherstrasse 9, D 97078 Wurzburg, Germany
J Biol Chem 279:37215-8. 2004..b>Fluorescence resonance energy transfer between variants of green fluorescent protein (enhanced cyan fluorescent protein and ..
- Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulinA Miyawaki
Department of Pharmacology, University of California, San Diego, La Jolla 92093 0647, USA
Nature 388:882-7. 1997..Binding of Ca2+ makes calmodulin wrap around the M13 domain, increasing the fluorescence resonance energy transfer (FRET) between the flanking GFPs...
- Fluorescent pigments in corals are photoprotectiveA Salih
School of Biological Sciences, The University of Sydney, New South Wales, Australia
Nature 408:850-3. 2000....
- A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteinsYurii G Yanushevich
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia
FEBS Lett 511:11-4. 2002..The elimination of basic residues located near the N-termini of FPs results in the generation of non-aggregating versions of several FPs, specifically, drFP583 (DsRed), DsRed-Timer, ds/drFP616, zFP506, zFP538, amFP486, and asFP595...
- Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cellsDavid A Zacharias
Department of Pharmacology, Biomedical Sciences Graduate Program, and, Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093 0647, USA
Science 296:913-6. 2002..b>Fluorescence resonance energy transfer measurements in living cells revealed that acyl but not prenyl modifications promote ..
- Aptamers switch on fluorescence of triphenylmethane dyesJeremy R Babendure
Department of Pharmacology, Howard Hughes Medical Institute, University of California San Diego, La Jolla, CA 92093 0647, USA
J Am Chem Soc 125:14716-7. 2003..sequences originally selected only for binding to malachite green or sulforhodamine B, can greatly enhance the fluorescence of normally nonfluorescent triphenylmethane dyes...
- Bimolecular fluorescence complementation: visualization of molecular interactions in living cellsTom K Kerppola
Department of Biological Chemistry, Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
Methods Cell Biol 85:431-70. 2008..The bimolecular fluorescence complementation (BiFC) assay provides a direct approach for the visualization of molecular interactions in ..
- Surgery with molecular fluorescence imaging using activatable cell-penetrating peptides decreases residual cancer and improves survivalQuyen T Nguyen
Department of Surgery, Howard Hughes Medical Institute, University of California at San Diego, La Jolla, CA 92093 0647, USA
Proc Natl Acad Sci U S A 107:4317-22. 2010..chelates enabled preoperative whole-body tumor detection by MRI, intraoperative guidance by real-time fluorescence, intraoperative histological analysis of margin status by fluorescence, and postoperative MRI tumor ..
- Tracking fluorescence-labeled rabies virus: enhanced green fluorescent protein-tagged phosphoprotein P supports virus gene expression and formation of infectious particlesStefan Finke
Max von Pettenkofer Institute and Gene Center, Feodor Lynen Strasse 25, D 81377 Munich, Germany
J Virol 78:12333-43. 2004..to enhanced green fluorescent protein (eGFP) can substitute for P throughout the viral life cycle, allowing fluorescence labeling and tracking of RV RNPs under live cell conditions...
- Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystalsDev K Chatterjee
Division of Bioengineering, National University of Singapore, Singapore 117574, Singapore
Biomaterials 29:937-43. 2008Upconversion fluorescence imaging technique with excitation in the near-infrared (NIR) region has been used for imaging of biological cells and tissues...
- Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imagingHui Wang Ai
University of Alberta, Department of Chemistry, Edmonton, Alberta, T6G 2G2 Canada
BMC Biol 6:13. 2008..To gain insight into the molecular basis for the blue-shifted fluorescence emission we undertook a mutagenesis-based study of residues in the immediate environment of the chromophore...
- Intraoperative evaluation of skin-flap viability using laser-induced fluorescence of indocyanine greenC Holm
Department of Plastic and Hand Surgery and Burn Centre, Technical University of Munich, Munich, Germany
Br J Plast Surg 55:635-44. 2002Laser-induced fluorescence of indocyanine green (ICG) is a new method for evaluating skin perfusion, which is superior to conventional fluorescein angiography...
- N-methyl-D-glucamine improves the laser-induced fluorescence capillary electrophoresis performance in the total plasma thiols measurementAngelo Zinellu
Chair of Clinical Biochemistry, University of Sassari, Viale San Pietro 43 B, I 07100 Sassari, Italy
Electrophoresis 24:2796-804. 2003We describe an ultrarapid capillary electrophoresis with laser-induced fluorescence (CE-LIF) method for total plasma thiols measurement...
- Diffusion dynamics of glycine receptors revealed by single-quantum dot trackingMaxime Dahan
Laboratoire Kastler Brossel, CNRS UMR 8552, Ecole Normale Supérieure and Université Pierre et Marie Curie, 24 rue Lhomond, 75005 Paris, France
Science 302:442-5. 2003..The entry of GlyRs into the synapse by diffusion was observed and further confirmed by electron microscopy imaging of QD-tagged receptors...
- Transcranial fluorescence imaging of auditory cortical plasticity regulated by acoustic environments in miceKuniyuki Takahashi
Department of Neurophysiology, Brain Research Institute, Niigata University, Japan
Eur J Neurosci 23:1365-76. 2006Functional brain imaging using endogenous fluorescence of mitochondrial flavoprotein is useful for investigating mouse cortical activities via the intact skull, which is thin and sufficiently transparent in mice...
- A protocol for cryoembedding the adult guinea pig cochlea for fluorescence immunohistologyBryony Coleman
The Department of Otolaryngology, University of Melbourne, East Melbourne 3002, Australia The Royal Victorian Eye and Ear Hospital, East Melbourne 3002, Australia
J Neurosci Methods 176:144-51. 2009..During our studies using the mouse embryonic stem cell line R1 B5-EGFP, we observed variable levels of fluorescence intensity of the GFP within these transfected cells...
- Considerations for the control of background fluorescence in clinical flow cytometryRuud Hulspas
Cytometry, Cytonome ST, LLC, Boston, Massachusetts 02210, USA
Cytometry B Clin Cytom 76:355-64. 2009Accurate measurement of antigen-positive cells by flow cytometry can be hampered by background fluorescence of antigen-negative cells and other particles (e.g., debris)...
- Delayed fluorescence as a universal tool for the measurement of circadian rhythms in higher plantsPeter D Gould
School of Biological Sciences, University of Liverpool, Crown Street, Liverpool, UK
Plant J 58:893-901. 2009..Delayed fluorescence (DF) is a fundamental process occurring in all photosynthetic organisms...
- Probing neuroserpin polymerization and interaction with amyloid-beta peptides using single molecule fluorescenceAlbert Chiou
Department of Chemistry, University of Cambridge, Cambridge, United Kingdom
Biophys J 97:2306-15. 2009..We have used a single molecule fluorescence method, two color coincidence detection, to determine the rate-limiting steps of the early stages of the ..
- DNA sequence detection using selective fluorescence quenching of tagged oligonucleotide probes by gold nanoparticlesHuixiang Li
Department of Chemistry, University of Rochester, Rochester, New York 14627, USA
Anal Chem 76:5414-7. 2004..Dye-tagged probe sequences have their fluorescence efficiently quenched when they are mixed with gold nanoparticles unless they hybridize with components of the ..
- In vivo near-infrared fluorescence imaging of integrin alphavbeta3 in brain tumor xenograftsXiaoyuan Chen
PET Imaging Science Center, University of Southern California Keck School of Medicine, Los Angeles, California, USA
Cancer Res 64:8009-14. 2004..1 +/- 5.6 nmol/L). The conjugate led to elevated cell-associated fluorescence on integrin-expressing tumor cells and endothelial cells and produced minimal cell fluorescence when ..
- Green fluorescent protein as a marker for gene expressionM Chalfie
Department of Biological Sciences, Columbia University, New York, NY 10027
Science 263:802-5. 1994..Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.
- Forced unfolding of proteins within cellsColin P Johnson
Biophysical Engineering Lab, University of Pennsylvania, Philadelphia, PA 19104, USA
Science 317:663-6. 2007..within stressed cells, in situ labeling of sterically shielded cysteines with fluorophores was analyzed by fluorescence imaging, quantitative mass spectrometry, and sequential two-dye labeling...
- Far-red fluorescent tags for protein imaging in living tissuesDmitry Shcherbo
Shemiakin Ovchinnikov Institute of Bioorganic Chemistry, Miklukho Maklaya 16 10, Moscow, Russia
Biochem J 418:567-74. 2009..also report tdKatushka2, a tandem far-red tag that performs well in fusions, provides 4-fold brighter near-IR fluorescence compared with mRaspberry or mCherry, and is 20-fold brighter than mPlum...
- Deep-tissue focal fluorescence imaging with digitally time-reversed ultrasound-encoded lightYing Min Wang
Bioengineering and Electrical Engineering, California Institute of Technology, 1200 E California Boulevard, Pasadena, California 91125, USA
Nat Commun 3:928. 2012b>Fluorescence imaging is one of the most important research tools in biomedical sciences. However, scattering of light severely impedes imaging of thick biological samples beyond the ballistic regime...
- Fluorescence quenching of dye molecules near gold nanoparticles: radiative and nonradiative effectsE Dulkeith
Photonics and Optoelectronics Group, Physics Department and CeNS, University of Munich, 80799 Munich, Germany
Phys Rev Lett 89:203002. 2002..chemically attached to differently sized gold nanoparticles, are investigated by means of time-resolved fluorescence experiments...
- Direct characterization of amyloidogenic oligomers by single-molecule fluorescenceAngel Orte
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom
Proc Natl Acad Sci U S A 105:14424-9. 2008..We describe here the application of a two-color single-molecule fluorescence technique to examine the assembly of oligomeric species formed during the aggregation of the SH3 domain of PI3 ..
- Bright monomeric red fluorescent protein with an extended fluorescence lifetimeEkaterina M Merzlyak
Evrogen JSC, Miklukho Maklaya 16 10, Moscow 117997, Russia
Nat Methods 4:555-7. 2007..red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability...
- Non-independence of Mnt repressor-operator interaction determined by a new quantitative multiple fluorescence relative affinity (QuMFRA) assayT K Man
Department of Genetics, Washington University Medical School, 660 S. Euclid, Box 8232, St Louis, MO 63110, USA
Nucleic Acids Res 29:2471-8. 2001..Using a newly developed high-throughput quantitative multiple fluorescence relative affinity (QuMFRA) assay, we directly quantified the relative equilibrium binding constants (K(ref)) of ..
- Fluorescence-based assay of sphingosine kinasesAndreas Billich
Novartis Research Institute Vienna, Brunner Strasse 59, A 1235 Vienna, Austria
Anal Biochem 326:114-9. 2004..We developed a fluorescence-based assay, using a sphingosine derivative labeled with a 7-nitrobenz-2-oxa-1,3-diazole moiety (15-NBD-Sph)...
- Control of stochasticity in eukaryotic gene expressionJonathan M Raser
Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California San Francisco UCSF, 600 16th Street, Room S472D, San Francisco, CA 94143 2240, USA
Science 304:1811-4. 2004..These mutations suggest that noise is an evolvable trait that can be optimized to balance fidelity and diversity in eukaryotic gene expression...
- Assignment of the low-temperature fluorescence in oxygen-evolving photosystem IIElmars Krausz
Research School of Chemistry, Australian National University, Building 35 Science Road, Canberra, ACT 0200, Australia
Photosynth Res 84:193-9. 2005Low-temperature absorption and fluorescence spectra of fully active cores and membrane-bound PS II preparations are compared. Detailed temperature dependence of fluorescence spectra between 5 and 70 K are presented as well as 1...
- Discovery metabolite profiling of the prolyl peptidasesAlan Saghatelian; Fiscal Year: 2009..Furthermore, the application of DMP to peptidases will demonstrate the generality of this approach for the future characterization of medically relevant enzymes and signaling pathways. ..
- Genetic variation and regulatory networks: Mechanisms and complexityDANA PE ER; Fiscal Year: 2009..These tools will be made publicly available, including a friendly graphical user interface and visualization. ..
- Novel TIRF microscopy analyzing trafficking & signaling at the cell cortexDerek Toomre; Fiscal Year: 2009..Consistent with the recovery act's goal, this funding will provide full-time summer employment for 4 undergraduate students and accelerate scientific achievement of the parent DP2 award...
- High Throughput Genotyping and DNA Sequencing for Studying the Genetic ContributiSonia Dua; Fiscal Year: 2013..ID numbers, number of labeled vials or plates of samples, 260/280 absorption readings and ratios, picogreen fluorescence readings or other methods of quantification (if required for the project), volume, DNA concentration, and ..
- Lauren Endres; Fiscal Year: 2014..To assess levels of DNA damage, we will use Fluorescence In Situ Hybridization (FISH) staining with H2AX to detect DNA strand breaks and use COMET assays as a ..
- Inhibition of human neutrophil function by Francisella tularensisLee Ann H Allen; Fiscal Year: 2012..Toward this end we will utilize chemiluminescence fluorescence and other biochemical assays to quantify reactive oxygen species;confocal microscopy to localize NADPH oxidase ..
- Investigating Mechanism of Intracellular Rotational Transport with Optical TJeffrey N Anker; Fiscal Year: 2012..sized tracer particles with one hemisphere coated by gold creating an orientation-dependent scattering and fluorescence signal...
- Structural and molecular basis of drug-induced IKACh reductionSAMI FOUAD NOUJAIM; Fiscal Year: 2012..To achieve my aims, I will use a multidisciplinary approach, involving fluorescence microscopy, chemiluminescence, NMR spectroscopy, X-ray crystallography and electrophysiology...
- SAMI FOUAD NOUJAIM; Fiscal Year: 2014..To achieve my aims, I will use a multidisciplinary approach, involving fluorescence microscopy, chemiluminescence, NMR spectroscopy, X-ray crystallography and electrophysiology...
- Laura L Dugan; Fiscal Year: 2014..A variety of techniques, including live animal fluorescence imaging of Nox2 activity, EPR, confocal imaging, immuno-fluorescence, electrophysiology and behavioral testing ..
- Monitoring cellular redox signaling and oxidant stress in vivoPaul T Schumacker; Fiscal Year: 2010..a protein that moves to a known intracellular compartment and signals a change in ROS levels by altering its fluorescence. We will turn this gene on in certain types of cells in the mouse, and measure the fluorescence changes using a ..
- Tissue-specific delivery of probes by control of membrane trafficking of endoprotBlake Peterson; Fiscal Year: 2010..By flanking members of the peptide library with red and green fluorophores, fluorescence resonance energy transfer (FRET) will allow quantification of in vivo cleavage of members of the peptide ..
- ARNULF HANS WERNER KOEPPEN; Fiscal Year: 2014..Regional increase can be determined directly by a new technology, high-definition X-ray fluorescence (HDXRF);and indirectly by a systematic examination of iron-responsive proteins...
- Epigenomic Events in Development of the Locus Coeruleus Noradrenergic NeuronsJan Krzysztof Blusztajn; Fiscal Year: 2012..We have developed a method to purify defined neuronal populations throughout lifespan by fluorescence-activated cell sorting using transgenic mice that express enhanced green fluorescent protein (EGFP) under the ..
- ALAN AI PING WEI; Fiscal Year: 2014..The level of aflatoxin is directly related to the fluorescence level of the PB tracer in a one-step assay...
- Interplay Between Tissue Architecture and Nuclear Organization in the DNA DamagePierre Alexandre Vidi; Fiscal Year: 2013..research, I have built a solid background in molecular and cellular biology and acquired extensive expertise in fluorescence techniques that will be applied to this project...
- Y S Prakash; Fiscal Year: 2016..Using biochemistry, pharmacology, molecular biology, immunocytochemistry, fluorescence Ca2+ imaging, force measurement techniques, and lung mechanics, we will focus on specific mechanisms that may ..
- Characterizing TLR Function of Human Dendritic Cells in the ElderlyAlexander Panda; Fiscal Year: 2012..The PI will employ cell biology techniques including pulse-chase experiments, confocal microscopy and fluorescence resonance energy transfer to evaluate age-associated changes in the kinetics of TLR1 surface expression, ..
- MOLECULAR BIOLOGY OF HUMAN COAGULATION FACTOR VThomas Ortel; Fiscal Year: 2010..Binding interactions will be characterized using quantitative fluorescence binding assays and functional prothrombinase assays...
- Dynamics of Kinesins, Dyneins, and MyosinsPaul R Selvin; Fiscal Year: 2010..and myosin V in peroxisomes, melanosomes, PC12 cells, and other cell lines, using single molecule FIONA (Fluorescence Imaging with One Nanometer Accuracy), and optical trapping...
- Robust Peptide-Based Diagnostics of Botulinum ToxinsJohn E Mueller; Fiscal Year: 2012..g., fluorescence or luminescence plate reader);and/or 5) trained personnel to execute assays...
- Dual-Receptor Targeted Nanoparticles for Photodynamic Therapy of Brain CancerAnn Marie Broome; Fiscal Year: 2013..We will perform state of the art in vitro and in vivo fluorescence imaging to characterize the delivery and targeting of the conjugates, as well as determining their therapeutic (..
- Ferritin: Protein/mRNA/DNA in Fe/O Regulation/MetabolismELIZABETH C contact THEIL; Fiscal Year: 2010..Analyze iron effects on FTL/FTH DNA 5mC. Techniques: Biochemistry (binding kinetics/equilibria, UV-vis fluorescence MCD/CD, NMR spectroscopies, and X-ray crystallography), Molecular Biology (in vitro translation, mutagenesis, ..
- Imaging Nonlinear Absorption of Biomarkers for Improved Detection of MelanomaWarren S Warren; Fiscal Year: 2010..but pigmented lesions present a horrible target because the generated light is reabsorbed (and in any event the fluorescence from melanin is extremely weak)...
- Salivary-based Bone-loss Marker Detection Platform for Point-of-Care ScreeningMANAL BESHAY; Fiscal Year: 2012..Highly stable quantum dot labeling techniques will provide detection via fluorescence measurements, and will allow multiplexed detection using the same sample, test strip, and excitation light ..
- A Computational Framework for Mapping Long Range Genetic CircuitsTolga Tasdizen; Fiscal Year: 2010..Anderson, 2009) developed a computational framework for 3-dimensional neurocircuitry of the retina and in fluorescence image reconstruction (Roysam), experts in non-human primate brain circuitry and neuroanatomy (Angelucci, Hof), ..
- EXPRESSION PROFILES FOR RET-POSITIVE DRG NEURONSSanjay Jain; Fiscal Year: 2013..made innovative Ret-reporter mice that constitutively or conditionally label Ret-expressing cells with green fluorescence protein for the first time...
- Haoxing Xu; Fiscal Year: 2016..to apply a multidisciplinary approach using electrophysiology, Ca2+ imaging, immunochemistry, biochemistry, and fluorescence imaging to test our central hypotheses that TRPML1 mediates cation efflux from endosomes and lysosomes and that ..
- Imbalances in Calcium Homeostasis: Mechanism Behind Ethanol-Related Dendritic DegCynthia A Dlugos; Fiscal Year: 2010..Levels of the SERCA 2b ATPase pump and calreticulin will be assessed with confocal microscopy, and fluorescence intensity correlation after treatment durations of 10, 20 or 40 weeks in proximal and distal dendritic branches,..
- Peter Sarnow; Fiscal Year: 2016..Specifically, we will employ biochemical, NMR and single-molecule fluorescence approaches to explore the timing and control of subunit joining on IRES-initiated mRNAs...
- Bingmei M Fu; Fiscal Year: 2014..Specific aims are: 1) use quantitative fluorescence video and confocal microscopy to determine the adhesion rates of normal, non-malignant (MCF-10A), and malignant ..
- KINETICS OF DRUG MACROMOLECULE COMPLEX FORMATIONPALMER WILLIAM TAYLOR; Fiscal Year: 2013..Conformation and dynamics are examined through fluorescence spectroscopy, decay of fluorescence anisotropy and H/D exchange...
- Hamid I Akbarali; Fiscal Year: 2014..We will also investigate the interaction of src kinase with the calcium channel using fluorescence resonance energy transfer (FRET) and siRNA approaches to delineate the functional effect of src-Ca2+ channel ..
- Elizabeth R Jacobs; Fiscal Year: 2016..The second is fluorescence optical imaging to follow the redox ratio (RR) of lung...
- JULIAN G CAMBRONERO; Fiscal Year: 2016..WASp, and the adaptors Grb2 and 14-3-3 through biochemical, genetic (Rac2-/-, WASp-/- and PLD2-/- KO mice) and fluorescence microscopy experiments...
- Carol L Wells; Fiscal Year: 2014..standard microbiological methods, confocal microscopy and high resolution electron microscopy, cell sorting, fluorescence in situ hybridization, and biochemical analyses...
- Zbigniew Darzynkiewicz; Fiscal Year: 2014..The first approach analyzes changes in the maximal pixel intensity of fluorescence of DNA-bound fluorescent ligands, a reporter of chromatin relaxation, the initial event in a cell's DNA ..
- Localization and characterization of the Clostridium difficile biofilmAdam Driks; Fiscal Year: 2013..Further, we used fluorescence in-situ hybridization to show that C...
- Regulation of the Endothelial Citrulline-Nitric Oxide CycleDuane C Eichler; Fiscal Year: 2010..transduction inhibitors in conjunction with siRNA;3) functional studies via enzyme activity and NO assays;4) fluorescence microscopy;5) real time PCR and western blotting;and 6) promoter analysis using mutagenesis, luciferase assays ..
- Site-directed Chemotherapy for Breast Cancer using Novel Angiogenesis InhibitorShaker A Mousa; Fiscal Year: 2010..Preliminary data using fluorescence microscopy showed that fluorescein-loaded TclNPs localize preferentially at the plasma membrane of the breast ..
- Eve Donnelly; Fiscal Year: 2016..research training in the Mentor's laboratory in techniques for analysis of collagen biochemistry using fluorescence and UPLC assays, as well as quantification of micro damage in bone tissue using mechanical testing and microCT...
- Fast accurate ultrasensitive in vitro assay to determine BoNT potency &stabilityARMIN H REITMAIR; Fiscal Year: 2012..It is based on alternating laser excitation (ALEX) single molecule fluorescence spectroscopy, whereby two (or more) target recognition molecules are tagged with different color fluorescence ..
- Theranostic Nanomedicine for Breast Cancer Prevention and Image-Guided TherapyPrakash R Rai; Fiscal Year: 2011..We will use a hyperspectral fluorescence microscope capable of non-invasively imaging BPD and ICG for simultaneous, background-free, quantitative in ..
- Biophysics of the morphology and motility of Borrelia burgdorferi in diverse enviCharles W Wolgemuth; Fiscal Year: 2013..Finally, modeling and time-lapse fluorescence microscopy will be used to determine the mechanisms of motility in epithelial cell layers, and in the tick and ..
- Monitoring of Cardiovascular Function in Infants By Transcutaneous Dye DilutionIstvan Seri; Fiscal Year: 2013..injection of the FDA-approved drug indocyanine green (ICG) and optical measurement of circulating blood ICG fluorescence. We now propose to develop and validate a skin probe, which can be used to measure cardiovascular parameters ..
- Visualization of ototoxic mitochondrial dysfunction in mammalian hair cellsShelley A Batts; Fiscal Year: 2012..To define the role of ototoxic mitochondrial dysfunction in hair cell apoptosis, I will use fluorescence microscopy techniques in adult guinea pigs in vivo and in postnatal rat cochlear cultures in vitro...
- Regulation of the Expression of G[alpha]i2 by Reactive Oxygen SpeciesIfeanyi J Arinze; Fiscal Year: 2012..Its nuclear translocation will be monitored by fluorescence imaging of transfected green fluorescence protein (GFP)-tagged Nrf2, and by site-directed mutagenesis to assess ..
- JONATHAN NOAH THON; Fiscal Year: 2014..Harvard University), has added to my repertoire a number of specialized cell biology techniques including fluorescence and electron microscopy, live cell imaging, retroviral infection, cell culture, and transgenic mouse models to ..
- ANP Receptor: Molecular approach of signaling mechanismsKailash N Pandey; Fiscal Year: 2013..aspects of NPRA including the dynamics of internalization, trafficking, sequestration, and recycling by fluorescence microscopy in living cells in real time, and 4) delineate the molecular determinants mediating post-binding ..
- The role of SIRT1/FOXO4 pathway in podocyte apoptosis of diabetes mellitusPETER YENLUNG CHUANG; Fiscal Year: 2013..effects of AGE and high glucose on oxidant stress will be quantified by flow-cytometric measurements of DCF-DA fluorescence and dimerization of homovanillic acid...
- David X Zhang; Fiscal Year: 2016..cultured endothelial cells using an integrated approach incorporating molecular biology, electrophysiology and fluorescence/electron imaging techniques with in vitro assessment of vessel reactivity...
- Development of Bioactive Chemical Probes for Calcium-activated Chloride ChannelMin Li; Fiscal Year: 2012..Lily Jan's laboratory, which first cloned the TMEM16A gene, we have developed several cell lines and a fluorescence-based iodide surrogate flux assay...
- KELLY ANNE BURKE; Fiscal Year: 2014..Microscopy (light, fluorescence, confocal) will be used to examine cellular organization and adipocyte size and lipid accumulation...
- ANDREW GEORGE MUNTEAN; Fiscal Year: 2014..MLL-PAFc binding will be monitored by fluorescence resonance energy transfer (FRET) and interaction of candidate chemical compounds will be verified by NMR ..
- Renate B Pilz; Fiscal Year: 2014..We will use co-immunoprecipitation, immuno- fluorescence staining, and bimolecular fluorescence complementation to characterize the PKG-regulated Src signaling complex, ..
- Post-translational regulation of MLL in leukemogenesisANDREW GEORGE MUNTEAN; Fiscal Year: 2012..MLL-PAFc binding will be monitored by fluorescence resonance energy transfer (FRET) and interaction of candidate chemical compounds will be verified by NMR ..
- COREY B SMITH; Fiscal Year: 2015..the execution of these aims, we employ state of the art electrophysiological, electrochemical and quantitative fluorescence microscopy as well as a newly-developed silicon nanowire-based field effect transistor (SiNW-FET) biosensor to ..
- Regulation of the VDJ recombinase during genotoxic stressKarla K Rodgers; Fiscal Year: 2013..In this project, we will use fluorescence microscopy combined with assays to monitor cellular protein-protein and protein-DNA interactions...
- Characterizing gene regulation with single molecule sensitive probesPhilip J Santangelo; Fiscal Year: 2013..it is our contention that when combined with antibody-based proximity ligation assays (PLAs) and multicolor fluorescence microscopy, single RNA-protein interactions can be detected in fixed tissue or post live-cell hybridization, ..
- Mechanism and therapy for arteriovenous malformationSuk Paul Oh; Fiscal Year: 2013..Deep-penetrating and noninvasive multiphoton fluorescence microscopy will be employed to capture 3-dimensional time lapse images blood vessels at subcellular level ..
- Jeffrey R Schelling; Fiscal Year: 2016..renal function by serum creatinine and uine albumin: creatinine ratios;for ex vivo NHE1 activity by fluorescence methods...
- Mark Lim; Fiscal Year: 2014..The strength of positive bait-prey interactions on the bead is quantitatively measured using fluorescence imaging and then the bead identity decoded using photocleavable mass tags combined with MALDI mass spectrometry ..
- 3D Skin Model to Test Toxic and Sensitizing Potentials of Environmental ChemicalsAkira Takashima; Fiscal Year: 2010..a HTP-compatible DC biosensor clone by engineering our skin-derived DC line XS106 to express the yellow fluorescence protein (YFP) gene under the control of the IL-1[unreadable] promoter...
- Point-of Care System for Determination of Bilirubin Capacity in NeonatesGLEN D RAMSAY; Fiscal Year: 2010..a point-of-care system (a small fluorometer and disposables) that makes use of bili- rubin's natural fluorescence. Bilirubin bound to albumin produces a fluorescence signal that can be detected in raw blood...
- Sub-Wavelength Imaging of Intracellular Metal IonsJoseph R Lakowicz; Fiscal Year: 2010..b>Fluorescence microscopy is one of the most widely used methods in the biological sciences...
- Live cell imaging of IgH and c-Myc gene loci and the role of nuclear organizationMark T Groudine; Fiscal Year: 2010..IgH/c-Myc translocations, the IgH and c-Myc loci will be imaged in live cells using 4D (3 dimensions over time) fluorescence microscopy...
- Regulation of Epithelial Sodium Channel by CFTR chloride channelBakhrom Berdiev; Fiscal Year: 2009..We will determine the CFTR-ENaC interaction using Fluorescence Resonance Energy Transfer (FRET) microscopy and co-immunoprecipitation (co-IP)...
- In Vivo Optical Imaging Scanner for Small Animal ResearchSTUART BERR; Fiscal Year: 2009..state of the art imaging devices including magnetic resonance (MR), X-ray computed tomography (CT), planar fluorescence and bioluminescence imaging (BLI), single gamma emission tomography (SPECT), and positron emission tomography (..
- FLUORESCENCE-BASED STUDIES OF SPHINGOMYELIN AND CERAMIDEEDWARD SCHUCHMAN; Fiscal Year: 2002..b>Fluorescence-based methods will be developed to determine whether pre-implantation stage embryos are affected with these ..
- Fluorescence Fluctuation Spectroscopy for von Willebrand Factor Multimer AnalysisMichael J Levene; Fiscal Year: 2010DESCRIPTION (provided by applicant): PROJECT SUMMARY We propose the development of fluorescence fluctuation spectroscopy (FFS) for the evaluation of von Willebrand Factor (vWF) multimers as a more accurate and reproducible technique in ..
- PROTOTYPE CATHETER FOR IN VIVO HIGH-RESOLUTION CARDIAC FLUORESCENCE MAPPINGMiguel Valderrabano; Fiscal Year: 2010The objective of this project is to develop a system to optically record fluorescence signals from heart tissues in vivo. Fluorescent dyes have been used to record biological processes from the heart for more than a decade...
- A feasibility study of genetically encoded fluorescent gold nanoclustersJan Liphardt; Fiscal Year: 2009..In vivo cluster growth will be monitored with fluorescence and electron microscopy. 3...
- Optical Fluorescence and X-Ray Computed Tomography Scanner for Small Animal In-ViSETH D SHULMAN; Fiscal Year: 2011..is to develop a quantitative in-vivo small animal imaging system for fluorescent reporter probes that fuses fluorescence light emitting computed tomography (FLECT) with X- ray computed tomography (CT)...
- Single Molecule Protein and Biopolymer Folding DynamicsShimon Weiss; Fiscal Year: 2003The objective of this proposal is to study protein folding using single-molecule fluorescence methodology. Understanding protein folding is fundamental to deciphering how the genetic code is translated into functional protein units...
- ACAS 570 ANCHORED CELL ANALYZER AND SORTERRAYMOND HESTER; Fiscal Year: 1991..with the capacity to perform the following functions not now available: (1) time- and spatially-resolved fluorescence analyses on individual cells, (2) the potential for studying cell-cell interactions at the level of the ..