mechanical stress

Summary

Summary: A purely physical condition which exists within any material because of strain or deformation by external forces or by non-uniform thermal expansion; expressed quantitatively in units of force per unit area.

Top Publications

  1. ncbi Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates
    N Q Balaban
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
    Nat Cell Biol 3:466-72. 2001
  2. ncbi Local force and geometry sensing regulate cell functions
    Viola Vogel
    Laboratory for Biologically Oriented Materials, Department of Materials, Swiss Federal Institute of Technology ETH, Zurich, Wolfgang Pauli Strasse 10, ETH Honggerberg, HCI F443, CH 8093 Zurich, Switzerland
    Nat Rev Mol Cell Biol 7:265-75. 2006
  3. pmc Cells lying on a bed of microneedles: an approach to isolate mechanical force
    John L Tan
    Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 100:1484-9. 2003
  4. ncbi The myofibroblast in wound healing and fibrocontractive diseases
    G Gabbiani
    Department of Pathology, CMU University of Geneva, 1 rue Michel Servet, 1211 Geneva 4, Switzerland
    J Pathol 200:500-3. 2003
  5. pmc Focal adhesion size controls tension-dependent recruitment of alpha-smooth muscle actin to stress fibers
    Jérôme M Goffin
    Laboratory of Cell Biophysics, Ecole Polytechnique Federale de Lausanne, CH 1015, Lausanne, Switzerland
    J Cell Biol 172:259-68. 2006
  6. pmc Integrin-mediated mechanotransduction requires its dynamic interaction with specific extracellular matrix (ECM) ligands
    S Jalali
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, CA 92093-0427, USA
    Proc Natl Acad Sci U S A 98:1042-6. 2001
  7. pmc Activation of integrins in endothelial cells by fluid shear stress mediates Rho-dependent cytoskeletal alignment
    E Tzima
    Department of Vascular Biology, The Scripps Research Institute, 10550 N.Torrey Pines Road, La Jolla, CA 92037, USA
    EMBO J 20:4639-47. 2001
  8. ncbi Cellular adaptation to mechanical stress: role of integrins, Rho, cytoskeletal tension and mechanosensitive ion channels
    Benjamin D Matthews
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA
    J Cell Sci 119:508-18. 2006
  9. pmc Micro-environmental mechanical stress controls tumor spheroid size and morphology by suppressing proliferation and inducing apoptosis in cancer cells
    Gang Cheng
    Edwin L Steele Laboratory of Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 4:e4632. 2009
  10. ncbi The differentiation of mesenchymal stem cells by mechanical stress or/and co-culture system
    I Chi Lee
    Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan, ROC
    Biochem Biophys Res Commun 352:147-52. 2007

Detail Information

Publications290 found, 100 shown here

  1. ncbi Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates
    N Q Balaban
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
    Nat Cell Biol 3:466-72. 2001
    ..The results put clear constraints on the possible molecular mechanisms for the mechanosensory response of focal adhesions to applied force...
  2. ncbi Local force and geometry sensing regulate cell functions
    Viola Vogel
    Laboratory for Biologically Oriented Materials, Department of Materials, Swiss Federal Institute of Technology ETH, Zurich, Wolfgang Pauli Strasse 10, ETH Honggerberg, HCI F443, CH 8093 Zurich, Switzerland
    Nat Rev Mol Cell Biol 7:265-75. 2006
    ..Recent tissue scaffolds that have been engineered at the micro- and nanoscale level now enable better dissection of the mechanosensing, transduction and response mechanisms...
  3. pmc Cells lying on a bed of microneedles: an approach to isolate mechanical force
    John L Tan
    Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 100:1484-9. 2003
    ....
  4. ncbi The myofibroblast in wound healing and fibrocontractive diseases
    G Gabbiani
    Department of Pathology, CMU University of Geneva, 1 rue Michel Servet, 1211 Geneva 4, Switzerland
    J Pathol 200:500-3. 2003
    ..The transition from fibroblasts to myofibroblasts is influenced by mechanical stress, TGF-beta and cellular fibronectin (ED-A splice variant)...
  5. pmc Focal adhesion size controls tension-dependent recruitment of alpha-smooth muscle actin to stress fibers
    Jérôme M Goffin
    Laboratory of Cell Biophysics, Ecole Polytechnique Federale de Lausanne, CH 1015, Lausanne, Switzerland
    J Cell Biol 172:259-68. 2006
    ..We propose that the different molecular composition and higher phosphorylation of FAs on supermature islets, compared with FAs on classical islets, accounts for higher stress resistance...
  6. pmc Integrin-mediated mechanotransduction requires its dynamic interaction with specific extracellular matrix (ECM) ligands
    S Jalali
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, CA 92093-0427, USA
    Proc Natl Acad Sci U S A 98:1042-6. 2001
    ..Our results indicate that the dynamic formation of new connections between integrins and their specific ECM ligands is critical in relaying the signals induced by shear stress to intracellular pathways...
  7. pmc Activation of integrins in endothelial cells by fluid shear stress mediates Rho-dependent cytoskeletal alignment
    E Tzima
    Department of Vascular Biology, The Scripps Research Institute, 10550 N.Torrey Pines Road, La Jolla, CA 92037, USA
    EMBO J 20:4639-47. 2001
    ..This transient inhibition is necessary for cytoskeletal alignment in the direction of flow. The results therefore define the role of integrins and Rho in a pathway leading to endothelial cell adaptation to flow...
  8. ncbi Cellular adaptation to mechanical stress: role of integrins, Rho, cytoskeletal tension and mechanosensitive ion channels
    Benjamin D Matthews
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA
    J Cell Sci 119:508-18. 2006
    To understand how cells sense and adapt to mechanical stress, we applied tensional forces to magnetic microbeads bound to cell-surface integrin receptors and measured changes in bead displacement with sub-micrometer resolution using ..
  9. pmc Micro-environmental mechanical stress controls tumor spheroid size and morphology by suppressing proliferation and inducing apoptosis in cancer cells
    Gang Cheng
    Edwin L Steele Laboratory of Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 4:e4632. 2009
    Compressive mechanical stress produced during growth in a confining matrix limits the size of tumor spheroids, but little is known about the dynamics of stress accumulation, how the stress affects cancer cell phenotype, or the molecular ..
  10. ncbi The differentiation of mesenchymal stem cells by mechanical stress or/and co-culture system
    I Chi Lee
    Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan, ROC
    Biochem Biophys Res Commun 352:147-52. 2007
    ..b>Mechanical stress affects the healing and remodeling process of ACL after surgery in important ways...
  11. ncbi Ligand-independent activation of vascular endothelial growth factor receptor 2 by fluid shear stress regulates activation of endothelial nitric oxide synthase
    Zheng Gen Jin
    Center for Cardiovascular Research and Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Circ Res 93:354-63. 2003
    ..Finally, we show that inhibiting VEGFR2 kinase significantly reduces flow-mediated NO-dependent arteriolar dilation in vivo. These data identify VEGFR2 as a key mechanotransducer that activates eNOS in response to blood flow...
  12. ncbi Mechanisms of force generation and transmission by myofibroblasts
    Boris Hinz
    Laboratory of Cell Biophysics, Swiss Federal Institute of Technology EPFL, Bâtiment SG AA B 143, CH 1015 Lausanne, Switzerland
    Curr Opin Biotechnol 14:538-46. 2003
    ..Regulation of these phenomena will be important for therapeutic strategies aimed at influencing fibrocontractive diseases...
  13. pmc Forced unfolding of proteins within cells
    Colin P Johnson
    Biophysical Engineering Lab, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 317:663-6. 2007
    ....
  14. pmc Mechanical stress-induced sarcomere assembly for cardiac muscle growth in length and width
    Brenda Russell
    Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, IL 60612, USA
    J Mol Cell Cardiol 48:817-23. 2010
    ..In this review, we link mechanotransduction to the molecular mechanisms for regulation of myocyte length and width...
  15. pmc Cell adhesion receptors in mechanotransduction
    Martin A Schwartz
    Department of Microbiology and Biomedical Engineering, Cardiovascular Research Center and Mellon Urological Cancer Research Institute, University of Virginia Charlottesville, VA 22908, USA
    Curr Opin Cell Biol 20:551-6. 2008
    ..Studies of developing organisms suggest that these mechanisms contribute to tissue level responses to tension and compression, thereby linking morphogenetic movements to cell fate decisions...
  16. ncbi Non-invasive axial loading of mouse tibiae increases cortical bone formation and modifies trabecular organization: a new model to study cortical and cancellous compartments in a single loaded element
    Roberto L de Souza
    Department of Veterinary Basic Sciences, Royal Veterinary College, Royal College Street, London NW1 0TU, UK
    Bone 37:810-8. 2005
    ....
  17. ncbi Cell differentiation by mechanical stress
    Gregory H Altman
    Department of Chemical and Biological Engineering, Bioengineering Center, Tufts University, Medford, Massachusetts 02155, USA
    FASEB J 16:270-2. 2002
    ..The application of mechanical stress over a period of 21 days up-regulated ligament fibroblast markers, including collagen types I and III and ..
  18. ncbi Cell mechanics: integrating cell responses to mechanical stimuli
    Paul A Janmey
    Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Annu Rev Biomed Eng 9:1-34. 2007
    ....
  19. pmc Mechanical tension controls granulation tissue contractile activity and myofibroblast differentiation
    B Hinz
    Department of Pathology, University of Geneva, Geneva, Switzerland
    Am J Pathol 159:1009-20. 2001
    ..Our results support the assumption that mechanical tension is crucial for myofibroblast modulation and for the maintenance of their contractile activity...
  20. pmc The NH2-terminal peptide of alpha-smooth muscle actin inhibits force generation by the myofibroblast in vitro and in vivo
    Boris Hinz
    Department of Pathology, Centre Medical Universitaire, University of Geneva, 1211 Geneva 4, Switzerland
    J Cell Biol 157:657-63. 2002
    ....
  21. ncbi Functional arteries grown in vitro
    L E Niklason
    Department of Anesthesia, Duke University, Durham, NC 27710, USA
    Science 284:489-93. 1999
    ..Tissue-engineered arteries were implanted in miniature swine, with patency documented up to 24 days by digital angiography...
  22. pmc Talin1 is critical for force-dependent reinforcement of initial integrin-cytoskeleton bonds but not tyrosine kinase activation
    Gregory Giannone
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    J Cell Biol 163:409-19. 2003
    ..Thus, force-dependent activation of tyrosine kinases is independent of early force-dependent structural changes that require talin1 as part of a critical scaffold...
  23. ncbi Involvement of CTGF in TGF-beta1-stimulation of myofibroblast differentiation and collagen matrix contraction in the presence of mechanical stress
    Qian Garrett
    Wound Healing Research Unit, Institute of Ophthalmology, London, United Kingdom
    Invest Ophthalmol Vis Sci 45:1109-16. 2004
    This study was undertaken to investigate the role of connective tissue growth factor (CTGF) in fibroblast-to-myofibroblast differentiation and fibroblast-mediated collagen matrix contraction in the presence of mechanical stress.
  24. ncbi Tensional homeostasis and the malignant phenotype
    Matthew J Paszek
    Department of Bioengineering, University of Pennsylvania, Philadelphia, 19104, USA
    Cancer Cell 8:241-54. 2005
    ..Thus, ERK and Rho constitute part of an integrated mechanoregulatory circuit linking matrix stiffness to cytoskeletal tension through integrins to regulate tissue phenotype...
  25. ncbi Integrins in mechanotransduction
    Akira Katsumi
    Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    J Biol Chem 279:12001-4. 2004
    ..We review the relationship between integrins and mechanical forces, the role of integrins in cellular responses to stretch and fluid flow, and propose that some of these events are mechanistically related...
  26. pmc Low-magnitude mechanical loading becomes osteogenic when rest is inserted between each load cycle
    Sundar Srinivasan
    Department of Orthopedics and Sports Medicine, University of Washington, Seattle 98104 2499, USA
    J Bone Miner Res 17:1613-20. 2002
    ..We conclude that 10 s of rest between each load cycle of a low-magnitude loading protocol greatly enhances the osteogenic potential of the regimen...
  27. ncbi Ischemia triggers BNP expression in the human myocardium independent from mechanical stress
    Helge Mollmann
    Kerckhoff Heart Center, Benekestrasse 2 8, 61231 Bad Nauheim, Germany
    Int J Cardiol 143:289-97. 2010
    ..Therefore, we investigated the BNP expression in isolated human muscle strips under conditions of ischemia with and without mechanical stress.
  28. ncbi Nonequilibrium mechanics of active cytoskeletal networks
    Daisuke Mizuno
    Department of Physics and Astronomy, Vrije Universiteit, 1081HV Amsterdam, Netherlands
    Science 315:370-3. 2007
    ..We present a quantitative theoretical model connecting the large-scale properties of this active gel to molecular force generation...
  29. ncbi Endothelial dysfunction, hemodynamic forces, and atherogenesis
    M A Gimbrone
    Department of Pathology, Brigham and Women s Hospital, Boston, Massachusetts 02115 5817, USA
    Ann N Y Acad Sci 902:230-9; discussion 239-40. 2000
    ....
  30. pmc Mechanical stress induces biotic and abiotic stress responses via a novel cis-element
    Justin W Walley
    Section of Plant Biology, University of California Davis, Davis, California, USA
    PLoS Genet 3:1800-12. 2007
    ..This structure suggests that plants may have a transcriptional network resembling the general stress signaling pathway in yeast and that the RSRE element may provide the key to this coordinate regulation...
  31. ncbi Mechanical stress activates angiotensin II type 1 receptor without the involvement of angiotensin II
    Yunzeng Zou
    Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine, 1 8 1 Inohana, Chuo Ku, Chiba 260 8670, Japan
    Nat Cell Biol 6:499-506. 2004
    ..Here we show that the AT1 receptor can be activated by mechanical stress through an angiotensin-II-independent mechanism...
  32. pmc Mechanical strain opens connexin 43 hemichannels in osteocytes: a novel mechanism for the release of prostaglandin
    Priscilla P Cherian
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78229 3900, USA
    Mol Biol Cell 16:3100-6. 2005
    ....
  33. pmc VEGF receptor 2 and the adherens junction as a mechanical transducer in vascular endothelial cells
    Ayelet Shay-Salit
    Department of Anatomy and Cell Biology, Bruce Rappaport Research Institute and the Rappaport Faculty of Medicine, Technion, Haifa 31096, Israel
    Proc Natl Acad Sci U S A 99:9462-7. 2002
    ..These results suggest that VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells...
  34. pmc The relationship between shear stress and flow-mediated dilatation: implications for the assessment of endothelial function
    Kyra E Pyke
    Human Vascular Control Laboratory, School of Physical and Health Education, Queen s University, Kingston, ON, Canada
    J Physiol 568:357-69. 2005
    ....
  35. ncbi Modulation of ERK 1/2 and p38 MAPK signaling pathways by ATP in osteoblasts: involvement of mechanical stress-activated calcium influx, PKC and Src activation
    S Katz
    Departamento de Biologia, Bioquimica y Farmacia, Universidad Nacional del Sur, San Juan 670, B8000ICN Bahia Blanca, Argentina
    Int J Biochem Cell Biol 38:2082-91. 2006
    ..We have found that when the cells are subsequently subjected to mechanical stress (medium perturbation), a transient calcium influx occurs...
  36. pmc Differential effect of steady versus oscillating flow on bone cells
    C R Jacobs
    Department of Orthopaedics and Rehabilitation, Penn State University, Hershey 17033, USA
    J Biomech 31:969-76. 1998
    ..Thus. these findings support the hypothesis that the response of bone cells to fluid flow is dependent on chemotransport effects...
  37. ncbi Unsteady and three-dimensional simulation of blood flow in the human aortic arch
    N Shahcheraghi
    Department of Mechanical and Aeronautical Engineering, Institute for Theoretical Dynamics, University of California, Davis 95616, USA
    J Biomech Eng 124:378-87. 2002
    ..Comparison of our numerical results with the localization of early atherosclerotic lesions broadly suggests preferential development of these lesions in regions of extrema (either maxima or minima) in wall shear stress and pressure...
  38. ncbi Mechanical stress-mediated Runx2 activation is dependent on Ras/ERK1/2 MAPK signaling in osteoblasts
    Takahiro Kanno
    Division of Oral and Maxillofacial Reconstructive Surgery, Department of Oral and Maxillofacial Surgery, Science of Physical Functions, Kyushu Dental College, Fukuoka, Japan
    J Cell Biochem 101:1266-77. 2007
    The sequence of biochemical events involved in mechanical stress-induced signaling in osteoblastic cells remains unclear...
  39. ncbi Mechanical strain induces specific changes in the synthesis and organization of proteoglycans by vascular smooth muscle cells
    R T Lee
    Cardiovascular Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, 02115, USA
    J Biol Chem 276:13847-51. 2001
    ..These data demonstrate that mechanical deformation increases specific vascular smooth muscle cell proteoglycan synthesis and aggregation, indicating a highly coordinated extracellular matrix response to biomechanical stimulation...
  40. pmc Mechanical strain injury increases intracellular sodium and reverses Na+/Ca2+ exchange in cortical astrocytes
    Candace L Floyd
    Department of Neurological Surgery, Center for Neuroscience, University of California, Davis, California 95616 8797, USA
    Glia 51:35-46. 2005
    ....
  41. ncbi Mechanosensitive ion channels: molecules of mechanotransduction
    Boris Martinac
    School of Medicine and Pharmacology, QEII Medical Centre, University of Western Australia, Crawley, WA 6009, Australia
    J Cell Sci 117:2449-60. 2004
    ..Moreover, cloning and sequencing of MS channels from several species has provided insights into their evolution, their physiological functions in prokaryotes and eukaryotes, and their potential roles in the pathology of disease...
  42. ncbi Uniaxial strain upregulates matrix-degrading enzymes produced by human vascular smooth muscle cells
    Kazuhiko Asanuma
    Division of Cardiology, Department of Medicine, Emory University School of Medicine, 1639 Pierce Drive, Atlanta, GA 30332, USA
    Am J Physiol Heart Circ Physiol 284:H1778-84. 2003
    ..We suggest that local increases in stationary mechanical strain resulting from stenting, hypertension, or atherosclerosis may lead to enhanced matrix degradation by SMC...
  43. pmc Biomechanical forces promote embryonic haematopoiesis
    Luigi Adamo
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 459:1131-5. 2009
    ..Collectively, these data reveal a critical role for biomechanical forces in haematopoietic development...
  44. pmc A sticky chain model of the elongation and unfolding of Escherichia coli P pili under stress
    Magnus Andersson
    Department of Physics, Umea University, SE 901 87 Umea, Sweden
    Biophys J 90:1521-34. 2006
    ..Entities such as the free energy, the stiffness, the elastic elongation, the opening length of the various bonds, and the number of PapA units in the rod are determined...
  45. ncbi Mechanical stress induces profound remodelling of keratin filaments and cell junctions in epidermolysis bullosa simplex keratinocytes
    David Russell
    Cancer Research UK Cell Structure Research Group, School of Life Sciences, MSI WTB complex, University of Dundee, Dundee, DD1 5EH, UK
    J Cell Sci 117:5233-43. 2004
    ..To investigate the part played by keratins in stress resilience, keratinocyte cell lines were subjected to mechanical stress. Repeated stretch and relaxation cycles over increasing time produced reproducible changes in the ..
  46. ncbi Functional living trileaflet heart valves grown in vitro
    S P Hoerstrup
    Department of Cardiovascular Surgery, Children s Hospital Boston, MA, USA
    Circulation 102:III44-9. 2000
    ..This study used an in vitro pulse duplicator system to provide a biomimetic environment during tissue formation to yield more mature implantable heart valves derived from autologous tissue...
  47. ncbi A finite element model of the human knee joint for the study of tibio-femoral contact
    Tammy L Haut Donahue
    Department of Mechanical Engineering, Michigan Technological University, Houghton 49931, USA
    J Biomech Eng 124:273-80. 2002
    ..However, unrealistic constraints on rotations other than flexion/extension can result in relatively large errors in contact variables...
  48. ncbi Mechanical stimuli to airway remodeling
    D J Tschumperlin
    Physiology Program, Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts, USA
    Am J Respir Crit Care Med 164:S90-4. 2001
    ..These findings demonstrate that mechanical stress may contribute to the remodeling of the asthmatic airway.
  49. pmc Myocardin-related transcription factor A is a common mediator of mechanical stress- and neurohumoral stimulation-induced cardiac hypertrophic signaling leading to activation of brain natriuretic peptide gene expression
    Koichiro Kuwahara
    Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Sakyo ku, Kyoto, Japan
    Mol Cell Biol 30:4134-48. 2010
    Subjecting cardiomyocytes to mechanical stress or neurohumoral stimulation causes cardiac hypertrophy characterized in part by reactivation of the fetal cardiac gene program...
  50. ncbi Differential responses of the Nrf2-Keap1 system to laminar and oscillatory shear stresses in endothelial cells
    Tomonori Hosoya
    Environmental Response Project ERATO Japan Science and Technology Agency, Graduate School of Comprehensive Human Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba, 305 8575, Japan
    J Biol Chem 280:27244-50. 2005
    ..These results suggest that O-flow inhibits Nrf2 activity at the DNA binding step, thereby suppressing athero-protective gene expression and hence predisposing the blood vessels to the formation of atherosclerosis...
  51. ncbi Global analysis of shear stress-responsive genes in vascular endothelial cells
    Norihiko Ohura
    Department of Biomedical Engineering, Graduate School of Medicine, University of Tokyo, Japan
    J Atheroscler Thromb 10:304-13. 2003
    ....
  52. ncbi Mechanical force-induced signal transduction in lung cells
    M Liu
    Thoracic Surgery Research Laboratory, Toronto General Hospital, University Health Network, Toronto M5G 2C4, Ontario, Canada M5G 1X8
    Am J Physiol 277:L667-83. 1999
    ..Further investigation in this exciting research field will facilitate our understanding of pulmonary physiology and pathophysiology at the cellular and molecular levels...
  53. pmc Mechanically induced osteogenic differentiation--the role of RhoA, ROCKII and cytoskeletal dynamics
    Emily J Arnsdorf
    Bone and Joint R and D Center, VA Palo Alto Health Care System, 3801 Miranda Avenue, Palo Alto, CA 94304, USA
    J Cell Sci 122:546-53. 2009
    ..Finally, we show RhoA activation and actin tension are negative regulators of both adipogenic and chondrogenic differentiation. However, an intact, dynamic actin cytoskeleton under tension is necessary for flow-induced gene expression...
  54. ncbi A mechanosensory complex that mediates the endothelial cell response to fluid shear stress
    Eleni Tzima
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 437:426-31. 2005
    ..Therefore, this mechanosensing pathway is required for the earliest-known events in atherogenesis...
  55. ncbi The cytoskeleton under external fluid mechanical forces: hemodynamic forces acting on the endothelium
    Brian P Helmke
    Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia 19104 6383, USA
    Ann Biomed Eng 30:284-96. 2002
    ..These quantitative studies strongly support the decentralized distribution of luminally imposed forces throughout the endothelial cell...
  56. ncbi Tensile properties of single stress fibers isolated from cultured vascular smooth muscle cells
    Shinji Deguchi
    Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University, Sendai 9808579, Japan
    J Biomech 39:2603-10. 2006
    ..These results suggest that SFs can bear intracellular stresses that may affect overall cell mechanical properties and will impact interpretation of intracellular stress distribution and force-transmission mechanism in adherent cells...
  57. ncbi Shear stress-mediated cytoskeletal remodeling and cortactin translocation in pulmonary endothelial cells
    Konstantin G Birukov
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    Am J Respir Cell Mol Biol 26:453-64. 2002
    ..Our results suggest a potential role for cortactin in the SS-induced EC cortical cytoskeletal remodeling and demonstrate a novel mechanism of Rac GTPase-dependent regulation of the pulmonary endothelial cytoskeleton by SS...
  58. pmc Conformational switch of angiotensin II type 1 receptor underlying mechanical stress-induced activation
    Noritaka Yasuda
    Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine, 1 8 1 Inohana, Chuo Ku, Chiba 260 8670, Japan
    EMBO Rep 9:179-86. 2008
    ..Our results show that the AT(1) receptor undergoes a conformational switch that couples mechanical stress-induced activation and inverse agonist-induced inactivation.
  59. pmc Positive end-expiratory pressure at minimal respiratory elastance represents the best compromise between mechanical stress and lung aeration in oleic acid induced lung injury
    Alysson Roncally S Carvalho
    Biomedical Engineering Program, COPPE, Federal University of Rio de Janeiro, Av, Horácio Macedo, CT Bloco H 327, 2030, 21941 914, Rio de Janeiro, Brazil
    Crit Care 11:R86. 2007
    ....
  60. pmc Roles of gap junctions and hemichannels in bone cell functions and in signal transmission of mechanical stress
    Jean Xin Jiang
    Department of Biochemistry, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229 3900, USA
    Front Biosci 12:1450-62. 2007
    ..Hemichannels in osteocytes mediate the immediate release of prostaglandins in response to mechanical stress. The major challenges remaining in the field are how the functions of these two types of channels are ..
  61. pmc Cell motility and local viscoelasticity of fibroblasts
    S Park
    Department of Physics, Texas Materials Institute, and Center for Nano and Molecular Science, University of Texas, Austin, Texas 78712, USA
    Biophys J 89:4330-42. 2005
    ..The correlation between the enhanced motility and the decrease in viscoelastic moduli supports the Elastic Brownian Ratchet model for driving lamellipodia extension...
  62. ncbi Shear stress inhibits adhesion molecule expression in vascular endothelial cells induced by coculture with smooth muscle cells
    Jeng Jiann Chiu
    Division of Medical Engineering Research, National Health Research Institutes, Taipei, Taiwan, Republic of China
    Blood 101:2667-74. 2003
    ..These coculture effects are inhibited by shear stress and require specific interaction between ECs and SMCs in close contact...
  63. ncbi Cell shape provides global control of focal adhesion assembly
    Christopher S Chen
    Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 2120, USA
    Biochem Biophys Res Commun 307:355-61. 2003
    ..This complex interplay between cell morphology, mechanics, and adhesion may be critical to how cells integrate from and function in living tissues...
  64. ncbi Identification of a 2-stage platelet aggregation process mediating shear-dependent thrombus formation
    Mhairi J Maxwell
    Australian Centre for Blood Diseases, Monash University, 89 Commercial Rd, Prahran, Australia, 3181
    Blood 109:566-76. 2007
    ..Moreover, they provide a model to explain how the discoid morphology of platelets facilitates the maintenance of adhesive interactions with thrombogenic surfaces under high shear stress conditions...
  65. ncbi Traction forces exerted through N-cadherin contacts
    Arthur Ganz
    INSERM, U706, Paris, F 75005 France
    Biol Cell 98:721-30. 2006
    ..The present study focuses on the determination of force transduction through cadherins at a single cell level...
  66. ncbi The structure and function of the endothelial glycocalyx layer
    Sheldon Weinbaum
    Department of Biomedical Engineering, The City College of New York, New York, NY 10031, USA
    Annu Rev Biomed Eng 9:121-67. 2007
    ....
  67. ncbi Shaken, not stirred: mechanical stress testing of an IgG1 antibody
    Sylvia Kiese
    Formulation R and D Biologics, Pharmaceutical and Analytical R and D, F Hoffmann La Roche Ltd, Basel, Switzerland
    J Pharm Sci 97:4347-66. 2008
    ..In this work, the aggregation behavior of a monoclonal antibody (IgG1) was investigated using two different mechanical stress methods namely stirring and shaking at two temperatures, various fill volumes and headspaces and different ..
  68. ncbi Osteopontin gene regulation by oscillatory fluid flow via intracellular calcium mobilization and activation of mitogen-activated protein kinase in MC3T3-E1 osteoblasts
    J You
    Musculoskeletal Research Laboratory, Department of Orthopaedics and Rehabilitation, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    J Biol Chem 276:13365-71. 2001
    ..In addition, experiments using a variety of pharmacological agents suggest that oscillatory flow induces Ca(2+)i mobilization via the L-type voltage-operated calcium channel and the inositol 1,4,5-trisphosphate pathway...
  69. ncbi Shear stress induces endothelial differentiation from a murine embryonic mesenchymal progenitor cell line
    Hao Wang
    Molecular Surgeon Research Center, Division of Vascular Surgery and Endovascular Therapy, Michael E DeBakey Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA
    Arterioscler Thromb Vasc Biol 25:1817-23. 2005
    ..Thus, we developed an in vitro shear stress system to examine the relationship between shear stress and the endothelial differentiation of a murine embryonic mesenchymal progenitor cell line, C3H/10T1/2...
  70. ncbi Effect of endothelial shear stress on the progression of coronary artery disease, vascular remodeling, and in-stent restenosis in humans: in vivo 6-month follow-up study
    Peter H Stone
    Cardiovascular Division, Brigham and Women s Hospital, 75 Francis St, Boston, Mass 02115, USA
    Circulation 108:438-44. 2003
    ..The endothelium controls local arterial responses by transduction of shear stress. Characterization of endothelial shear stress (ESS) may allow for prediction of progression of atherosclerosis and in-stent restenosis...
  71. ncbi Prolonged fluid shear stress induces a distinct set of endothelial cell genes, most specifically lung Krüppel-like factor (KLF2)
    Rob J Dekker
    Department of Biochemistry, Academic Medical Center, University of Amsterdam, The Netherlands
    Blood 100:1689-98. 2002
    ..To date LKLF is the first endothelial transcription factor that is uniquely induced by flow and might therefore be at the molecular basis of the physiological healthy, flow-exposed state of the endothelial cell...
  72. ncbi Effects of shear stress on 3-D human mesenchymal stem cell construct development in a perfusion bioreactor system: Experiments and hydrodynamic modeling
    Feng Zhao
    Department of Chemical and Biomedical Engineering, FAMU FSU College of Engineering, Florida State University, Tallahassee, FL 32310, USA
    Biotechnol Bioeng 96:584-95. 2007
    ..These results demonstrate that shear stress is an important microenvironment parameter that regulates hMSC construct development at a range significantly lower than those reported previously in the perfusion system...
  73. ncbi Development-related changes in the expression of shear stress responsive genes KLF-2, ET-1, and NOS-3 in the developing cardiovascular system of chicken embryos
    Bianca C W Groenendijk
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 230:57-68. 2004
    ..Our results suggest that, in the embryonic cardiovascular system, KLF-2 is expressed in regions of highest shear stress, and that ET-1 and NOS-3 expression, at least in the later stages, is related to shear stress...
  74. pmc Cell-cell mechanical communication through compliant substrates
    Cynthia A Reinhart-King
    Department of Biomedical Engineering, Cornell University, Ithaca, New York 14853, USA
    Biophys J 95:6044-51. 2008
    ..These findings are critical for the understanding of cell-cell adhesion during tissue formation and disease progression, and for the design of biomaterials intended to support both cell-matrix and cell-cell adhesion...
  75. ncbi Buonocore memorial lecture. Adhesion to enamel and dentin: current status and future challenges
    Bart Van Meerbeek
    Leuven BIOMAT Research Cluster, Department of Conservative Dentistry, School of Dentistry, Oral Pathology and Maxillo Facial Surgery, Catholic University of Leuven, Belgium
    Oper Dent 28:215-35. 2003
    ..Especially "mild," two-step self-etch adhesives that bond through a combined micromechanical and chemical interaction with tooth tissue closely approach conventional three-step systems in bonding performance...
  76. ncbi Shear stress regulation of Krüppel-like factor 2 expression is flow pattern-specific
    Nanping Wang
    Department of Bioengineering, Whitaker Institute of Biomedical Engineering, University of California San Diego, La Jolla, CA 92093 0412, USA
    Biochem Biophys Res Commun 341:1244-51. 2006
    ..In conclusion, differential regulation of KLF2 may mediate the distinct vascular effects induced by various patterns of shear stress...
  77. ncbi Cervical spondylotic myelopathy associated with kyphosis or sagittal sigmoid alignment: outcome after anterior or posterior decompression
    Kenzo Uchida
    Department of Orthopaedics and Rehabilitation Medicine, Fukui University Faculty of Medical Sciences, Eiheiji, Fukui, Japan
    J Neurosurg Spine 11:521-8. 2009
    The effects of sagittal kyphotic deformities or mechanical stress on the development of cervical spondylotic myelopathy, or the reduction and fusion of kyphotic sagittal alignment have not been consistently documented...
  78. ncbi Wall shear stress--an important determinant of endothelial cell function and structure--in the arterial system in vivo. Discrepancies with theory
    Robert S Reneman
    Department of Physiology, Cardiovascular Research Institute Maastricht, University of Maastricht, Maastricht, The Netherlands
    J Vasc Res 43:251-69. 2006
    ..Applications of wall shear stress as in flow-mediated arterial dilation, clinically in use to assess endothelial cell (dys)function, are also addressed. This review starts with some background considerations and some theoretical aspects...
  79. ncbi Signaling of mechanical stretch in human keratinocytes via MAP kinases
    S Kippenberger
    Department of Dermatology and Venerology, University Hospital, Frankfurt Main, Germany
    J Invest Dermatol 114:408-12. 2000
    ..It is likely that alterations of skin mechanics in vivo underlying pathogenic processes like wound formation and healing trigger physiologic responses via the MAP kinase pathway...
  80. ncbi Heparan sulfate proteoglycan is a mechanosensor on endothelial cells
    Jeffry A Florian
    Biomolecular Transport Dynamics Laboratory, Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, USA
    Circ Res 93:e136-42. 2003
    ..The full text of this article is available online at http://www.circresaha.org...
  81. ncbi The role of shear stress in the pathogenesis of atherosclerosis
    Kristopher S Cunningham
    Department of Pathology, Toronto General Research Institute, University Health Network, Canada
    Lab Invest 85:9-23. 2005
    ..Thus, shear stress is critically important in regulating the atheroprotective, normal physiology as well as the pathobiology and dysfunction of the vessel wall through complex molecular mechanisms that promote atherogenesis...
  82. ncbi Aging enhances a mechanically-induced reduction in tendon strength by an active process involving matrix metalloproteinase activity
    Jayesh Dudhia
    Department of Veterinary Clinical Sciences, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UK
    Aging Cell 6:547-56. 2007
    ....
  83. ncbi Mechanical stress-induced DNA damage and rac-p38MAPK signal pathways mediate p53-dependent apoptosis in vascular smooth muscle cells
    Manuel Mayr
    Institute for Biomedical Aging Research, Austrian Academy of Sciences, Innsbruck, Austria
    FASEB J 16:1423-5. 2002
    ..FASEB J. 2000; 15:261-270). In this article we investigated the molecular mechanisms of mechanical stress-induced apoptosis...
  84. ncbi Mechanical stress directly suppresses osteoclast differentiation in RAW264.7 cells
    Nobumitsu Suzuki
    Department of Oral Diagnosis and Oral Medicine, Hokkaido University Graduate School of Dental Medicine, Sapporo 060 8586, Japan
    Int J Mol Med 21:291-6. 2008
    Although it is known that mechanical stress to osteoblast and periodontal ligament cells suppresses osteoclast differentiation, little is known about the direct effect of mechanical stress on osteoclast differentiation...
  85. ncbi Mechanical stress promotes odontoblastic differentiation via the heme oxygenase-1 pathway in human dental pulp cell line
    Sun Kyung Lee
    Department of Oral and Maxillofacial Pathology, College of Dentistry, Wonkwang University, Iksan, South Korea
    Life Sci 86:107-14. 2010
    ..is involved in osteoblastic differentiation, the HO-1- and odontoblastic differentiation-inducing effects of mechanical stress (MS) have not been clarified in human dental pulp cells (HDPCs)...
  86. ncbi Regulation of extracellular matrix synthesis by mechanical stress
    M Chiquet
    M E Müller Institut für Biomechanik, Universitat Bern, Switzerland
    Biochem Cell Biol 74:737-44. 1996
    ..Similar enhancer elements might be present in the promoters of other genes induced by mechanical stress. It can be speculated that connective tissue cells sense force vectors in their ECM environment and react to ..
  87. ncbi Mechanical stress-evoked but angiotensin II-independent activation of angiotensin II type 1 receptor induces cardiac hypertrophy through calcineurin pathway
    Ning Zhou
    Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, China
    Biochem Biophys Res Commun 397:263-9. 2010
    b>Mechanical stress can induce cardiac hypertrophy through angiotensin II (AngII) type 1 (AT(1)) receptor independently of AngII, however, the intracellular mechanisms remain largely indeterminate...
  88. pmc beta-Arrestin-biased agonism of the angiotensin receptor induced by mechanical stress
    Kriti Rakesh
    Department of Medicine, Duke University Medical Center, DUMC 3104, 226 CARL Building, Durham, NC 27710, USA
    Sci Signal 3:ra46. 2010
    ..These data show that the heart responds to acute increases in mechanical stress by activating beta-arrestin-mediated cell survival signals.
  89. ncbi Determination of mechanical stress distribution in Drosophila wing discs using photoelasticity
    Ulrike Nienhaus
    Fachbereich Physik, Universitat Konstanz, Universitatstrasse 10, Fach 688, 78457 Konstanz, Germany
    Mech Dev 126:942-9. 2009
    ..In order to study the connections between mechanical stress, growth control and morphogenesis, information about the distribution of stress in a tissue is invaluable...
  90. pmc Effects of cell tension on the small GTPase Rac
    Akira Katsumi
    Division of Vascular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 158:153-64. 2002
    ..We hypothesize that regulation of Rac activity by tension may be important for motility, polarization, and directionality of cell movement...
  91. ncbi Who's afraid of the big bad Wolff?: "Wolff's law" and bone functional adaptation
    Christopher Ruff
    Center for Functional Anatomy and Evolution, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Am J Phys Anthropol 129:484-98. 2006
    ..Thus, when employed with appropriate caution, these features may be used to reconstruct mechanical loadings and behavioral differences within and between past populations...
  92. pmc Enhancement of osteoclastic bone resorption and suppression of osteoblastic bone formation in response to reduced mechanical stress do not occur in the absence of osteopontin
    M Ishijima
    Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo 101 0062, Japan
    J Exp Med 193:399-404. 2001
    Reduced mechanical stress to bone in bedridden patients and astronauts leads to bone loss and increase in fracture risk which is one of the major medical and health issues in modern aging society and space medicine...
  93. ncbi Regulation of GADD153 induced by mechanical stress in cardiomyocytes
    W P Cheng
    Taipei Medical University, Taipei, Taiwan
    Eur J Clin Invest 39:960-71. 2009
    ..However, the expression of GADD153 in cardiomyocytes under mechanical stress is little known...
  94. pmc Stiffness and fraction of Myosin motors responsible for active force in permeabilized muscle fibers from rabbit psoas
    Marco Linari
    Laboratorio di Fisiologia, Dipartimento di Biologia Animale e Genetica, Universita degli Studi di Firenze, Firenze, Italy
    Biophys J 92:2476-90. 2007
    ..The finding that the fraction of myosin heads attached to actin in an isometric contraction is 0.33 rules out the hypothesis of multiple mechanical cycles per ATP hydrolyzed...
  95. ncbi Role of the actin cytoskeleton in tuning cellular responses to external mechanical stress
    M B Asparuhova
    Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
    Scand J Med Sci Sports 19:490-9. 2009
    ..We describe examples of genes that are regulated by mechanical stress in a manner depending on actin dynamics, among them the ECM protein, tenascin-C.
  96. ncbi Mechanotransduction and the glycocalyx
    J M Tarbell
    Biomedical Engineering Department, The City College of New York, CUNY, New York, NY 10031, USA
    J Intern Med 259:339-50. 2006
    ..This review focuses on mechanotransduction of shear stress by ECs and considers the evidence in support of the surface glycocalyx acting as a mechanotransducer...
  97. ncbi Periodontal ligament cells under mechanical stress induce osteoclastogenesis by receptor activator of nuclear factor kappaB ligand up-regulation via prostaglandin E2 synthesis
    Hiroyuki Kanzaki
    Department of Life Long Oral Health Science, Graduate School of Dentistry, Tohoku University, Sendai, Japan
    J Bone Miner Res 17:210-20. 2002
    ..From this, we hypothesized that PDL cells under mechanical stress should play a pivotal role in osteoclast formation during orthodontic tooth movement...
  98. ncbi FAK mediates the activation of cardiac fibroblasts induced by mechanical stress through regulation of the mTOR complex
    Ana Paula Dalla Costa
    Department of Internal Medicine, School of Medicine and Department of Cell Biology, State University of Campinas, Campinas, SP, Brazil
    Cardiovasc Res 86:421-31. 2010
    Cardiac fibroblasts are activated by mechanical stress, but the underlying mechanisms involved remain poorly understood...
  99. ncbi Epithelial Na(+) channels are regulated by flow
    L M Satlin
    Department of Pediatrics, Mount Sinai School of Medicine, New York 10029 6574, USA
    Am J Physiol Renal Physiol 280:F1010-8. 2001
    ..We propose that intraluminal flow rate may be an important regulator of channel activity in the CCD...
  100. ncbi Transcriptional induction of FosB/DeltaFosB gene by mechanical stress in osteoblasts
    Daisuke Inoue
    Department of Medicine and Bioregulatory Sciences, University of Tokushima Graduate School of Medicine, 3 18 15 Kuramoto cho, Tokushima 770 8503, Japan
    J Biol Chem 279:49795-803. 2004
    b>Mechanical stress to bone plays a critical role in maintaining bone mass and strength. However, the molecular mechanism of mechanical stress-induced bone formation is not fully understood...
  101. pmc Basic FGF mediates an immediate response of articular cartilage to mechanical injury
    Tonia Vincent
    The Kennedy Institute of Rheumatology, Faculty of Medicine, Imperial College School of Science, Technology, and Medicine, 1 Aspenlea Road, London W6 8LH, UK
    Proc Natl Acad Sci U S A 99:8259-64. 2002
    ..The strong induction of matrix metalloproteinases and tissue inhibitor of metalloproteinases-1 suggests that bFGF could have a role in remodeling damaged tissue...

Research Grants69

  1. Mechanical Stress and the Heat-Treatment of Soft Tissue
    JAY HUMPHREY; Fiscal Year: 2003
    ..There is a need for a firmer scientific understanding. ..
  2. Pathogenic Mechanisms of PBEF in Ventilator Induced Lung Injury
    Joe G N Garcia; Fiscal Year: 2012
    ..Aim 1 will functionally characterize key regulatory elements which drive VILI-mediated PBEF expression (mechanical stress- and ROS-responsive promoter elements, 5'SNPs)...
  3. Role of the Renin-Angiotensin System in the Regulation of Biliary Proliferation
    SHANNON STROUD GLASER; Fiscal Year: 2010
    ..We have also obtained unique in vitro preliminary data demonstrating that mechanical stress, which occurs during extrahepatic obstructive disorders, induces cholangiocyte proliferation via mechanical ..
  4. Mechanical and Osmotic Signaling in Chondrocytes
    Farshid Guilak; Fiscal Year: 2013
    ..maintenance of the cartilage extracellular matrix by chondrocytes, and under abnormal conditions, mechanical stress may be a significant factor in the initiation and progression of joint degeneration...
  5. Structure and function of stress fibers and cell adhesion sites
    Mary C Beckerle; Fiscal Year: 2013
    ..physiological functions associated with respiration, circulation, locomotion, and digestion expose cells to mechanical stress that can deform or damage cytoskeletal elements...
  6. Obesity, Biomechanics, and Inflammation in Osteoarthritis
    Farshid Guilak; Fiscal Year: 2013
    ..In Aim 3, we will use controlled in vitro models of cartilage explant loading to examine the effects of mechanical stress in combination with pro- inflammatory cytokines and fatty acids on the anabolic and catabolic activities of ..
  7. The Role of Xanthine Oxidoreductase in Ventilator-Associated Lung Injury
    Paul M Hassoun; Fiscal Year: 2013
    ..In preliminary experiments that form the basis of this application renewal, we now demonstrate that mechanical stress, in the context of high tidal volume ventilation, leads to profound re-arrangement of the pulmonary ..
  8. Syndecan-1 in Aortic Aneurysm Formation
    Elliot Chaikof; Fiscal Year: 2012
    ..we speculate that macrophage syndecan-1 shed in response to pro-inflammatory stimuli, such as elevated local mechanical stress or oxidized lipids, will preferentially bind and effectively sequester chemokines and proteases relevant to ..
  9. Physiology of Respiratory Muscle Micromechanics
    ALADIN BORIEK; Fiscal Year: 2009
    ..diaphragm;each of these proteins has a distinct signaling role, which may depend on the direction of applied mechanical stress. Each of the proteins is involved in the initiation of a specific signal transduction pathway that affects ..
  10. Nuclear mechanics and mechanotransduction in muscular laminopathies
    Jan Lammerding; Fiscal Year: 2013
    ..phenotypes and to explore to what extent impaired nuclear structure and altered cellular sensitivity to mechanical stress contribute to the muscle-specific phenotypes...
  11. Protein Therapeutics for Muscular Dystrophy
    Norio Takizawa; Fiscal Year: 2013
    ..to increase the capacity of muscle cells to reseal their membranes following physiological levels of mechanical stress could address both of these mechanisms leading to improvement of the regenerative capacity in muscular ..
  12. Mechanically Stressed VSMC: A Role for Slingshot Phosphatase in Inflammation
    Alejandra San Martin; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Cell and tissues are subjected to different types of mechanical stress. Hypertension is a pathological state in which the increase in pressure induces enhanced mechanical forces within the vessel ..
  13. COX Inhibition & Musculoskeletal Responses to Exercise
    Wendy M Kohrt; Fiscal Year: 2010
    ..prostaglandins (PG) are important factors in the bone formation and muscle protein synthesis responses to mechanical stress (i.e., exercise) and that cyclooxygenase (COX) is a key enzyme for mechanically-induced PG synthesis...
  14. Disease Mechanisms in ARVC
    Jeffrey E Saffitz; Fiscal Year: 2013
    ..Thus, we will test the hypothesis that mechanical stress, such as might occur following strenuous or prolonged exercise, destabilizes desmosomes in the heart (..
  15. CYTOSKELETAL REGULATION OF LUNG ENDOTHELIAL PATHOBIOLOGY
    Joe G N Garcia; Fiscal Year: 2012
    ..Project #3 examines the effects of mechanical stress (cyclic stretch) on EC signaling to the cytoskeleton in the context of barrier dysfunction/restoration via ..
  16. REGULATION AND FUNCTION OF KERATINS IN THE EPIDERMIS
    DENNIS ROOP; Fiscal Year: 2010
    ..the keratin filament cytoskeleton is crucial to the structural integrity of epithelial tissues exposed to mechanical stress. The identification of mutations causing these disorders has improved our understanding of keratin structure ..
  17. Novel Methodology for Rapid Antibiotic Susceptibiility Testing in S. aureus
    Alexis F Sauer Budge; Fiscal Year: 2010
    ..Brigham and Women's Hospital and Harvard Medical School have teamed up to explore the hypothesis that mechanical stress on bacteria will damage the cell wall and rapidly initiate cell wall repair biosynthesis pathways...
  18. Intrinsic Repair of the Knee Meniscus
    Farshid Guilak; Fiscal Year: 2011
    ..Our prior work has demonstrated that mechanical stress alters the metabolism of menisci, inducing production of nitric oxide (NO) and prostaglandin E2 (PGE2) ..
  19. Microfluidic Tissue Engineering of Small Airway Injuries
    Shuichi Takayama; Fiscal Year: 2010
    ..mechanical stresses and damage airway epithelial cells, and that even normally sub-lethal amounts of fluid mechanical stress will become lethal in the presence of other insults such as bacteria or hyperoxia-mediated inflammation, ..
  20. Protein Therapeutics for Muscular Dystrophy
    Norio Takizawa; Fiscal Year: 2010
    ..to increase the capacity of muscle cells to reseal their membranes following physiological levels of mechanical stress could address both of these mechanisms leading to improvement of the regenerative capacity in muscular ..
  21. Signals for Post-pneumonectomy Compensatory Lung Growth
    Connie C W Hsia; Fiscal Year: 2013
    ..Lobar expansion may also alter and interact with regional perfusion. Relationship of in vivo tissue mechanical stress to cellular response has not been established, but the hypoxia-inducible factor-erythropoietin-vascular ..
  22. MAP Kinase Signaling in Apoptosis-Induced Ventilator-Associated Lung Injury
    Mahendra Damarla; Fiscal Year: 2013
    ..of HSP27 is critical for promoting HVT MV mediated apoptosis and resultant pulmonary edema using an in vitro mechanical stress model and an established murine model of VALI in wild type and MK2-/- mice...
  23. Role of mechanosensation in retinal function and dysfunction
    David Krizaj; Fiscal Year: 2013
    ..the cellular response in this disease but could also play a key function in other diseases that involve mechanical stress in the retina, such as diabetic retinopathy, ischemia and macular edema...
  24. Activation of sirtuins to prevent adverse cardiac remodeling after CABG
    Mahesh P Gupta; Fiscal Year: 2013
    ..Both human and animal studies have demonstrated that biochemical and mechanical stress on the heart leads to cardiac myocyte hypertrophy and cardiac fibroblasts (CF) differentiation to ..
  25. Myocyte specific regulation of metabolism and the response to biomechanical force
    Monte S Willis; Fiscal Year: 2013
    ..These studies will identify for the first time how mechanical stress from a variety of cardiac pathologies leads to characteristic changes in fatty acid and glucose oxidation ..
  26. XOR Regulation of Endothelial Cell Apoptosis in the Model of VALI
    BO SOO KIM; Fiscal Year: 2010
    ..pre-treatment of EC in vitro prevents CS induced apoptosis;however, the mechanisms by which XOR potentiates mechanical stress induced apoptosis are not well described and are the focus of this study...
  27. Schwann Cell Control of Chronic Nerve Injury
    Ranjan Gupta; Fiscal Year: 2013
    ..of extracellular signals from CNC injury into intracellular molecular pathways;possible signals include mechanical stress and ischemia contributing independently or synergistically...
  28. Mechanical stress and skeletal plasticity after spinal cord injury in humans
    Richard K Shields; Fiscal Year: 2013
    ..Aim 2 will determine the effects of low load vibratory mechanical stress (30 Hz, 0.6 g,) on bone density of the tibia over 1 year following SCI...
  29. METABOLISM OF THE TRABECULAR MESHWORK
    David L Epstein; Fiscal Year: 2011
    ..Project Summary: This grant has focused in the past on determining the potential role of factors induced by mechanical stress in trabecular meshwork (TM) cells as modulators of Schlemm's canal (SC) endothelium permeability and ..
  30. Extra / Intra-cellular Signaling in Novel 3-Dimensional Human Leiomyoma Cultures
    WILLIAM HENRY CATHERINO; Fiscal Year: 2013
    ..on ECM formation and dissolution on 3-D leiomyoma cultures, and (2) to confirm that perturbations in mechanical stress through formed ECM alter Rho/ERK signaling and increase aberrant ECM formation...
  31. The Effects of Altered Contractility on Cardiac Myocyte Signaling and Hypertrophy
    MARIA LYNN VALENCIK; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Pressure and volume overload in the heart causes mechanical stress in cardiac tissue, which in turn leads to altered phenotypes of cardiac myocytes...
  32. Thermodynamics and kinetics of DNA-ligand binding probed by DNA overstretching
    Andreas Hanke; Fiscal Year: 2013
    ..force experiments, a poor understanding of the structural and thermodynamic response of biomolecules to mechanical stress has limited the insight that such experiments have provided into helix destabilization and DNA-ligand ..
  33. Nanomechanics of the extracellular matrix
    Julio M Fernandez; Fiscal Year: 2013
    ..HEALTH RELEVANCE: The elasticity of healthy and diseased tissues is determined by how proteins respond to mechanical stress. It is then of great medical importance to understand the nanomechanical properties of proteins...
  34. Mechanisms Underlying Muscle Development in Drosophila
    ERIKA RAE GEISBRECHT; Fiscal Year: 2013
    ..In healthy muscle tissue, the MTJ provides resistance against the mechanical stress generated during muscle contraction, and it is now known that any decrease in MTJ stability leads to muscle ..
  35. Cardiac Structure and Function in Early Familial Cardiomyopathy
    Janaka Wansapura; Fiscal Year: 2013
    ..In aim 3, the relevance of increased left ventricular mechanical stress in the regional myocardial dysfunction and the development of fibrosis in aortic banded and un-operated Scgd-..
  36. The Role of Cyclooxygenase 2 in Mechanical Stress-Induced Lung Injury
    Stephanie A Nonas; Fiscal Year: 2013
    ..However, little is known about the expression of C0X2 in response to mechanical stress and its involvement in mechanical stress-induced lung injury...
  37. Mechanosensitivity of Cell Membranes: Role of Lipid-Protein Interactions
    Mirianas Chachisvilis; Fiscal Year: 2012
    ..e. changes in physical properties of the membrane under mechanical stress can regulate activity of membrane proteins coupled to intracellular signaling pathways...
  38. Revisiting the bacterial cell wall as a target for new antibiotics
    DOUGLAS BENJAMIN WEIBEL; Fiscal Year: 2011
    ..The cell wall functions as the 'cytoskeleton'in bacteria and protects cells from mechanical stress. The enormous osmotic pressure across the cell wall (>1 atm) requires that cell growth be tightly ..
  39. Biomechanical Mechanisms of Artery Tortuosity
    Hai Chao Han; Fiscal Year: 2013
    ..The two specific aims are to determine what types of blood flow, mechanical stress, and wall property changes initiate artery buckling and how buckling affects arterial blood flow and wall ..
  40. Genetic dissection of the role of chondroitin sulfate in cartilage
    Yu Yamaguchi; Fiscal Year: 2013
    ..In Aim 2, we will employ an in vitro mechanical stress model to characterize cell death and antioxidant responses of CS-deficient chondrocytes to mechanical stress...
  41. Noise-induced acute hair cell Death
    Bo Hua Hu; Fiscal Year: 2013
    ..hair cell death and evaluate the effect of a membrane repair strategy on the hair cell's response to mechanical stress, (2) to determine the effect of the disruption of membrane adhesion on the cytoskeletal structure of ..
  42. Imaging protease activation in calcific aortic valve disease
    Mehran M Sadeghi; Fiscal Year: 2013
    ..b>Mechanical stress, atherosclerotic risk factors, deposition of lipid particles, and other factors induce focal inflammation ..
  43. Alternative treatments for disc degeneration: Effects on matrix homeostasis
    Gwendolyn A Sowa; Fiscal Year: 2013
    ..Key mechanisms involved in this process include inflammatory and mechanical stress. It is biologically plausible that CAM therapies, such as yoga or nutritional supplements, may exert their ..
  44. Bacterial Attachment under Mechanical Perturbations
    Jorge Alegre-Cebollada; Fiscal Year: 2013
    ..Bacteria colonizing the respiratory tract are adapted to attachment under mechanical stress caused by sliding mucus and coughing...
  45. Mechanical and Dynamical Regulation of Protein Kinases
    CHRISTOPHER LEE MCCLENDON; Fiscal Year: 2013
    ..A covariance of mechanical stress approach will be developed along with transfer entropy analysis in internal coordinates to identify ..
  46. Estrogen Receptor Beta Regulation of Mandibular Condylar Growth
    Sunil Wadhwa; Fiscal Year: 2013
    ..by regulating chondrogenesis and endochondral ossification, in order to achieve a better balance between mechanical stress and the load bearing capacity of the joint...
  47. Identification of genes responsible for sarcolemmal integrity in C. elegans
    Hongkyun Kim; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Many types of cells in metazoan species operate under conditions of mechanical stress, and are evolved to cope with plasma membrane damage...
  48. Role of matrix shear stress in annulus fibrosus cell mechanobiology
    Adam H Hsieh; Fiscal Year: 2012
    ..There is strong evidence from the literature that indicate that mechanical stress plays a role in the progression of DDD...
  49. Developing a device for visualization of large-scale cryopreservation
    Yoed Rabin; Fiscal Year: 2012
    ..high cooling rate required for vitrification drives another potentially devastating effect, which is thermo-mechanical stress (driven by the tendency of the material to contract with the decreasing temperature), with fracture ..
  50. Biophysical Modeling of Fluid and Solute Transport in the TMJ Disc
    Hai Yao; Fiscal Year: 2010
    ..a new multiphasic mechano-electrochemical finite element model of the TMJ disc which will provide details of mechanical stress, strain, fluid pressure, nutrient concentrations, electrical potential, fluid flow, and transport of ..
  51. Solid State NMR Structure/Function Studies of Amelogenin
    Wendy J Shaw; Fiscal Year: 2013
    ..Enamel is the most highly ordered biomineralization crystal and is uniquely designed to handle abrasions and mechanical stress. Enamelins, tuftelins, ameloblastins and amelogenins are proteins present during enamel formation and all ..
  52. Local Biomechanics of Median Nerve Insult in Carpal Tunnel Syndrome
    THOMAS DUDLEY BROWN; Fiscal Year: 2010
    ..The central paradigm of the proposed research is that local mechanical stress on the median nerve can be meaningfully quantified...
  53. Impact of Fluid versus Solid Stresses on Air-Blood Integrity
    Nicholas J Douville; Fiscal Year: 2010
    ..Solid mechanical stress (volutrauma) occurs as epithelial cells are exposed to the cyclic stretching and relaxation of the alveolus ..
  54. In Vivo Dynamic Lumbar Vertebral Motion and Disc Deformation During Lifting Tasks
    Xudong Zhang; Fiscal Year: 2012
    ..e., dynamic postures), and begin to develop an improved model for evaluating the low back mechanical stress associated with manual tasks...
  55. BIOMECHANICAL EVALUATION OF ABDOMINAL AORTIC ANEURYSM
    DAVID ALAN VORP; Fiscal Year: 2010
    ..AAA rupture occurs when the mechanical stress acting on the aneurysm wall locally exceeds its failure strength...
  56. STRUCTURAL DYNAMICS OF ACTOMYOSIN MOTILITY
    Yale E Goldman; Fiscal Year: 2013
    ..optical trap will be combined with single-molecule polTIRF microscopy to directly evaluate the influence of mechanical stress, strain, and flexibility on stepping rates and protein orientation changes that relate to chemo-mechanical ..
  57. Stretch Dependent Signaling in Dystrophic Skeletal Muscle
    CHRISTOPHER WILLIAM WARD; Fiscal Year: 2013
    ..While substantial evidence exists for an enhancement in mechanical stress dependent activation of reactive oxygen signaling (ROS) and calcium influx pathways in the mdx, little ..
  58. Lung Perfusion Heterogeneity and Mechanisms of Edema
    Susan R Hopkins; Fiscal Year: 2010
    ..b>Mechanical stress injury of the pulmonary capillaries has been shown to be important in the development of HAPE, but how the ..
  59. Using in vitro approaches to improve muscle regrowth in atrophied older humans
    MICHAEL JOHN STEC; Fiscal Year: 2013
    ..will determine key translational signaling processes associated with impaired protein synthesis responses to mechanical stress in myotubes from nonresponders...
  60. Development and testing of a law for morphomechanics at multiple scales
    Matthew A Wyczalkowski; Fiscal Year: 2012
    ..The proposed morphomechanical law states that tissue, when subject to mechanical stress, responds according to either the hyper-restoration (HR) or the stretch activation (SA) response, and the ..
  61. NEUROTRANSMITTERS AND CYTOKINES IN TOOTH MOVEMENT
    ZEEV DAVIDOVITCH; Fiscal Year: 1992
    ..and circulatory cells modulate the response be periodontal ligament (PDL) and alveolar bone cells to mechanical stress, particularly as it is applied during orthodontic tooth movement...
  62. ARTICULAR CARTILAGE REPAIR
    Robert Sah; Fiscal Year: 1999
    ..Using an in vitro model of cartilage repair, the applicant proposes to determine the role of static mechanical stress in modulating the integrative repair process...
  63. Biologic scaffold prostheses to enhance meniscus repair
    JOE WEINBERG; Fiscal Year: 2009
    ..These scaffolds provide physiologic mechanical properties prior to implantation. We have also shown that mechanical stress and cytokines alter the metabolism of menisci and inhibit repair of meniscal injury...
  64. Targeting Membrane Repair in Muscular Dystrophy
    Noah Weisleder; Fiscal Year: 2013
    ..to increase the capacity of muscle cells to reseal their membranes following physiological levels of mechanical stress could address both of these mechanisms leading to improvement of muscular dystrophy pathology...
  65. THE ROLE OF WALL STRESS DISTRIBUTION IN ABDOMINAL AORTIC
    Mark Fillinger; Fiscal Year: 2001
    ..The basic premise is that rupture occurs when the mechanical stress (i.e...
  66. Pulsed Electron Avalanche Knife: Intraocular Microsurg.
    Daniel Palanker; Fiscal Year: 2006
    ..This device will also be applied for capsulotomy with no risk of rupture and mechanical stress to zonular fibers exerted during capsulorhexis...
  67. Acetaminophen and Impaired Musculoskeletal Adaptations to Exercise Training
    CATHERINE JANKOWSKI; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): Mechanical stress (e.g., exercise) triggers an increase in prostaglandins (PCs) in skeletal muscle and bone through activation of the enzyme cyclooxygenase (COX)...
  68. Thermo mechanical stress in cryopreserved blood vessels
    Yoed Rabin; Fiscal Year: 2006
    ..Although the experimental work in this study is targeted to blood vessels, the results of this study could be expanded and become useful for a wide variety of cryopreserved natural tissues and engineered constructs. ..
  69. Intraocular Pressure-Mediated .Damage to the Optic Nerve
    Eric Nauman; Fiscal Year: 2004
    ..Elevated pressure increases the mechanical stress in the sclera and the support structures surrounding the optic nerve head including the lamina cribrosa...