precipitation

Summary

Top Publications

  1. ncbi Polyphenol/peptide binding and precipitation
    Adrian J Charlton
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S102TN, United Kingdom
    J Agric Food Chem 50:1593-601. 2002
  2. ncbi Comparison of protein precipitation methods for sample preparation prior to proteomic analysis
    Lei Jiang
    Bio X Life Science Research Center, Shanghai Jiao Tong University, Hao Ran Building, P O Box 501, 1954 Hua Shan Road, Shanghai 200030, PR China
    J Chromatogr A 1023:317-20. 2004
  3. ncbi Selenite precipitation by a rhizospheric strain of Stenotrophomonas sp. isolated from the root system of Astragalus bisulcatus: a biotechnological perspective
    Simona Di Gregorio
    Department of Science and Technology, Laboratories of Microbial Biotechnology and Environmental Microbiology, University of Verona, Strada Le Grazie 15 Ca Vignal, 37134Verona, Italy
    Environ Int 31:233-41. 2005
  4. ncbi Facile RAFT precipitation polymerization for the microwave-assisted synthesis of well-defined, double hydrophilic block copolymers and nanostructured hydrogels
    Zesheng An
    Department of Chemistry and Biochemistry, University of California, Santa Barbara, California 93106 9510, USA
    J Am Chem Soc 129:14493-9. 2007
  5. doi Design of equidistant and revert type precipitation patterns in reaction-diffusion systems
    Ferenc Molnar
    Eotvos University, Institute of Chemistry, Budapest, Hungary
    Phys Chem Chem Phys 10:2368-73. 2008
  6. ncbi Theoretical analysis of calcium phosphate precipitation in simulated body fluid
    Xiong Lu
    Department of Mechanical Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Biomaterials 26:1097-108. 2005
  7. ncbi A modified protein precipitation procedure for efficient removal of albumin from serum
    Yi Yun Chen
    Core Facilities for Proteomic Research, Taipei, Taiwan
    Electrophoresis 26:2117-27. 2005
  8. ncbi Combined use of crystalline salt forms and precipitation inhibitors to improve oral absorption of celecoxib from solid oral formulations
    Héctor R Guzmán
    TransForm Pharmaceuticals, Inc, 29 Hartwell Avenue, Lexington, Massachusetts 02421, USA
    J Pharm Sci 96:2686-702. 2007
  9. ncbi Partitioning and inactivation of viruses by the caprylic acid precipitation followed by a terminal pasteurization in the manufacturing process of horse immunoglobulins
    M Mpandi
    R and D, Serolab s a, Chemin de la Vulliette 4, CP 36, 1000 Lausanne 25, Switzerland
    Biologicals 35:335-41. 2007
  10. ncbi In vitro Ca-P precipitation on biodegradable thermoplastic composite of poly(epsilon-caprolactone-co-DL-lactide) and bioactive glass (S53P4)
    T Jaakkola
    Department of Prosthetic Dentistry and Biomaterials Research, Institute of Dentistry, University of Turku, Lemminkaisenkatu 2, 20520 Turku, Finland
    Biomaterials 25:575-81. 2004

Detail Information

Publications273 found, 100 shown here

  1. ncbi Polyphenol/peptide binding and precipitation
    Adrian J Charlton
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S102TN, United Kingdom
    J Agric Food Chem 50:1593-601. 2002
    ..Astringency arises from precipitation of polyphenol/peptide complexes, which is an important protective mechanism in animals that consume ..
  2. ncbi Comparison of protein precipitation methods for sample preparation prior to proteomic analysis
    Lei Jiang
    Bio X Life Science Research Center, Shanghai Jiao Tong University, Hao Ran Building, P O Box 501, 1954 Hua Shan Road, Shanghai 200030, PR China
    J Chromatogr A 1023:317-20. 2004
    ..find the most efficient method for sample preparation, we used human plasma and compared four widely applied precipitation methods, using trichloroacetic acid (TCA), acetone, chloroform/methanol and ammonium sulfate, as well as ..
  3. ncbi Selenite precipitation by a rhizospheric strain of Stenotrophomonas sp. isolated from the root system of Astragalus bisulcatus: a biotechnological perspective
    Simona Di Gregorio
    Department of Science and Technology, Laboratories of Microbial Biotechnology and Environmental Microbiology, University of Verona, Strada Le Grazie 15 Ca Vignal, 37134Verona, Italy
    Environ Int 31:233-41. 2005
    ....
  4. ncbi Facile RAFT precipitation polymerization for the microwave-assisted synthesis of well-defined, double hydrophilic block copolymers and nanostructured hydrogels
    Zesheng An
    Department of Chemistry and Biochemistry, University of California, Santa Barbara, California 93106 9510, USA
    J Am Chem Soc 129:14493-9. 2007
    Water-soluble macromolecular chain transfer agents (Macro-CTAs) were developed for the microwave-assisted precipitation polymerization of N-isopropylacrylamide...
  5. doi Design of equidistant and revert type precipitation patterns in reaction-diffusion systems
    Ferenc Molnar
    Eotvos University, Institute of Chemistry, Budapest, Hungary
    Phys Chem Chem Phys 10:2368-73. 2008
    ..The precipitation process proved itself to be a good candidate for building complex structures...
  6. ncbi Theoretical analysis of calcium phosphate precipitation in simulated body fluid
    Xiong Lu
    Department of Mechanical Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Biomaterials 26:1097-108. 2005
    The driving force and nucleation rate of calcium phosphate (Ca-P) precipitation in simulated body fluid (SBF) were analyzed based on the classical crystallization theory...
  7. ncbi A modified protein precipitation procedure for efficient removal of albumin from serum
    Yi Yun Chen
    Core Facilities for Proteomic Research, Taipei, Taiwan
    Electrophoresis 26:2117-27. 2005
    ..gel electrophoresis (1-D SDS-PAGE) and used as the first screening strategy to evaluate and optimize for the precipitation experimental conditions...
  8. ncbi Combined use of crystalline salt forms and precipitation inhibitors to improve oral absorption of celecoxib from solid oral formulations
    Héctor R Guzmán
    TransForm Pharmaceuticals, Inc, 29 Hartwell Avenue, Lexington, Massachusetts 02421, USA
    J Pharm Sci 96:2686-702. 2007
    ..salts in novel solid formulations, which were designed to simultaneously facilitate dissolution and inhibit precipitation in vitro, showed fast and complete absorption in beagle dogs at doses up to 7.5 mg/kg orally...
  9. ncbi Partitioning and inactivation of viruses by the caprylic acid precipitation followed by a terminal pasteurization in the manufacturing process of horse immunoglobulins
    M Mpandi
    R and D, Serolab s a, Chemin de la Vulliette 4, CP 36, 1000 Lausanne 25, Switzerland
    Biologicals 35:335-41. 2007
    ..Exploiting these two properties, we sequentially used the caprylic acid precipitation and the pasteurization to purify horse hyperimmune globulins used in the manufacturing of Sérocytol...
  10. ncbi In vitro Ca-P precipitation on biodegradable thermoplastic composite of poly(epsilon-caprolactone-co-DL-lactide) and bioactive glass (S53P4)
    T Jaakkola
    Department of Prosthetic Dentistry and Biomaterials Research, Institute of Dentistry, University of Turku, Lemminkaisenkatu 2, 20520 Turku, Finland
    Biomaterials 25:575-81. 2004
    ..The calcium phosphate precipitation and apatite formation were evaluated by scanning electron microscopy (SEM) and infra-red spectroscopy (IR) ..
  11. pmc Uranyl precipitation by Pseudomonas aeruginosa via controlled polyphosphate metabolism
    Neil Renninger
    Department of Chemical Engineering, University of California at Berkeley, Berkeley, CA 94720 1462, USA
    Appl Environ Microbiol 70:7404-12. 2004
    ..and time-resolved laser-induced fluorescence spectroscopy (TRLFS) suggest that this removal was due to the precipitation of uranyl phosphate at the cell membrane...
  12. ncbi Heparin-mediated extracorporeal low density lipoprotein precipitation as a possible therapeutic approach in preeclampsia
    Ying Wang
    Institute of Clinical Chemistry, University Hospital Grosshadern, Marchioninistrasse 15, 81377 Munich, Germany
    Transfus Apher Sci 35:103-10. 2006
    ..Heparin-mediated extracorporeal low density lipoprotein (LDL) precipitation (H.E.L.P...
  13. ncbi Struvite precipitation thermodynamics in source-separated urine
    Mariska Ronteltap
    Swiss Federal Institute of Aquatic Science and Technology, Eawag, 8600 Dubendorf, Switzerland
    Water Res 41:977-84. 2007
    Struvite (MgNH(4)PO(4).6H(2)O) precipitation eliminates phosphate efficiently from urine, a small but highly concentrated stream in the total flux of domestic wastewater...
  14. ncbi Phosphate and potassium recovery from source separated urine through struvite precipitation
    J A Wilsenach
    Delft University of Technology, Department of Biotechnology, Julianalaan 67, 2628BC, Delft, The Netherlands
    Water Res 41:458-66. 2007
    ..higher phosphate concentration than sludge reject water, allowing simpler (and less expensive) process for precipitation of phosphates...
  15. doi Micronization of atorvastatin calcium by antisolvent precipitation process
    Hai Xia Zhang
    Key Lab for Nanomaterials, Ministry of Education, Beijing University of Chemical Technology, Beijing, PR China
    Int J Pharm 374:106-13. 2009
    Amorphous atorvastatin calcium (AC) ultrafine powder has been successfully prepared by antisolvent precipitation and spray drying process, in which hydroxypropyl methylcellulose (HPMC) was employed to control the particle size and ..
  16. ncbi Comparison of a homogeneous assay with a precipitation method for the measurement of HDL cholesterol in diabetic patients
    Tonny Jensen
    Steno Diabetes Center, Gentofte, Denmark
    Diabetes Care 25:1914-8. 2002
    To compare direct-measured HDL cholesterol with HDL cholesterol measured by a precipitation method.
  17. ncbi Calcium phosphate precipitation method for the treatment of dentin hypersensitivity
    Toshiyuki Suge
    Department of Conservative Dentistry, School of Dentistry, Tokushima University, Tokushima, Japan
    Am J Dent 15:220-6. 2002
    To evaluate the feasibility of the calcium phosphate precipitation (CPP) method as a treatment for dentin hypersensitivity using vital teeth of beagle dogs...
  18. doi Heat-induced transfer of protons from chitosan to glycerol phosphate produces chitosan precipitation and gelation
    Marc Lavertu
    Institute of Biomedical Engineering, Department of Chemical Engineering, Ecole Polytechnique de Montreal, P O Box 6079, Station Centre Ville, Montreal, Quebec, Canada H3C 3A7
    Biomacromolecules 9:640-50. 2008
    ..Subsequently, an investigation of ionization and precipitation behavior of chitosan, including dependencies on temperature, added salt, and fraction of deacetylated monomers ..
  19. ncbi Fabrication of fast responsive, thermosensitive poly(N-isopropylacrylamide) hydrogels by using diethyl ether as precipitation agent
    Xiao Ding Xu
    Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan 430072, People s Republic of China
    J Biomed Mater Res A 86:1023-32. 2008
    ..the properties of thermosensitive poly(N-isopropylacrylamide) (PNIPAAm) hydrogel by using diethyl ether as a precipitation agent during the polymerization/crosslinking...
  20. pmc Linked redox precipitation of sulfur and selenium under anaerobic conditions by sulfate-reducing bacterial biofilms
    Simon L Hockin
    Division of Environmental and Applied Biology, Biological Sciences Institute, School of Life Sciences, University of Dundee, Dundee DD1 4HN, Scotland, United Kingdom
    Appl Environ Microbiol 69:7063-72. 2003
    ..Elemental selenium and elemental sulfur were precipitated outside SRB cells. Precipitation occurred by an abiotic reaction with bacterially generated sulfide...
  21. ncbi Evidence for surface precipitation of phosphate on goethite
    Adeline Ler
    Chemical and Environmental Engineering Department, National University of Singapore, 5 Engineering Drive 2, Singapore 117576
    Environ Sci Technol 37:2694-700. 2003
    ..have suggested that the interaction between phosphate and goethite includes ternary adsorption/ surface precipitation as well as surface complexformation...
  22. ncbi Dissolution and absorption modeling: model expansion to simulate the effects of precipitation, water absorption, longitudinally changing intestinal permeability, and controlled release on drug absorption
    Kevin C Johnson
    Intellipharm, LLC, Niantic, Connecticut 06357, USA
    Drug Dev Ind Pharm 29:833-42. 2003
    ..beyond the original application of simulating immediate-release dosage forms to include simulation of drug precipitation, water absorption from the gastrointestinal tract, changing gastrointestinal permeability, disintegration, and ..
  23. ncbi Purification of histidine-tagged single-chain Fv-antibody fragments by metal chelate affinity precipitation using thermoresponsive copolymers
    Ashok Kumar
    Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P O Box 124, SE 22100 Lund, Sweden
    Biotechnol Bioeng 84:494-503. 2003
    Metal chelate affinity precipitation (MCAP) has been successfully developed as a simple purification process for proteins that have affinity for metal ions...
  24. ncbi Use of seeds to control precipitation of calcium carbonate and determination of seed nature
    Marcel Donnet
    Ecole Polytechnique Federale de Lausanne, Powder Technology Laboratory, Materials Institute, CH 1015 Lausanne, Switzerland
    Langmuir 21:100-8. 2005
    Understanding and controlling precipitation reactions is a major challenge for industrial crystallization. Calcium carbonate is a widely studied system: more than 3000 papers have been devoted to the subject over the past 10 years...
  25. pmc Trichloroacetic acid-induced protein precipitation involves the reversible association of a stable partially structured intermediate
    Dakshinamurthy Rajalingam
    Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, USA
    Protein Sci 18:980-93. 2009
    Sample preparation for proteomic analysis involves precipitation of protein using 2,2,2-trichloroacetic acid (TCA). In this study, we examine the mechanism of the TCA-induced protein precipitation reaction...
  26. doi Precipitation of heavy metals from wastewater using simulated flue gas: sequent additions of fly ash, lime and carbon dioxide
    Quanyuan Chen
    School of Environmental Science and Engineering, Donghua University, 2999 Renmin Road, Shanghai 201620, PR China
    Water Res 43:2605-14. 2009
    ..to an acceptable level for discharge, in this work, fly ash was added as a seed material to enhance lime precipitation and the suspension was exposed to CO2 gas...
  27. ncbi Preparation of ethyl cellulose/methyl cellulose blends by supercritical antisolvent precipitation
    Ana Rita C Duarte
    Nutraceuticals and Delivery Laboratory, ITQB IBET, Aptd 12, 2781 901 Oeiras, Portugal
    Int J Pharm 311:50-4. 2006
    ..The microspheres were precipitated from a mixture of dichloromethane (DCM) and dimethylsulfoxide (DMSO) (4:1 ratio). The best process conditions for this mixture were according to our study 40 degrees C and 80 bar...
  28. doi Purification of DNA from the cell-associated herpesvirus Marek's disease virus for 454 pyrosequencing using micrococcal nuclease digestion and polyethylene glycol precipitation
    Jeremy D Volkening
    Southeast Poultry Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Athens, GA 30605, USA
    J Virol Methods 157:55-61. 2009
    ..weight viral DNA is purified away from an excess of degraded cellular DNA using polyethylene glycol precipitation. 454-based pyrosequencing of viral DNA purified in this manner has generated data containing as little as 2...
  29. pmc Se(VI) reduction and the precipitation of Se(0) by the facultative bacterium Enterobacter cloacae SLD1a-1 are regulated by FNR
    N Yee
    Department of Environmental Sciences, Rutgers University, 14 College Farm Rd, New Brunswick, NJ 07102, USA
    Appl Environ Microbiol 73:1914-20. 2007
    ..fate of selenium in the environment is controlled, in part, by microbial selenium oxyanion reduction and Se(0) precipitation. In this study, we identified a genetic regulator that controls selenate reductase activity in the Se-reducing ..
  30. doi Proteome profile of human breast cancer tissue generated by LC-ESI-MS/MS combined with sequential protein precipitation and solubilization
    Yan Gong
    PolyomX Program, Cross Cancer Institute, Alberta Cancer Board, and Department of Chemistry, Oncology, and Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada
    J Proteome Res 7:3583-90. 2008
    ..The complexity of the breast cancer tissue proteome was reduced by using protein precipitation from a tissue extract, followed by sequential protein solubilization in solvents of different solubilizing ..
  31. ncbi Removal of phospholipid contaminants through precipitation of glycosylphosphatidylinositols
    Nahid Azzouz
    Institut fur Virologie, AG Parasitologie, Zentrum für Hygiene und Med Mikrobiologie, Philipps Universitat Marburg, 35037 Marburg, Germany
    Anal Biochem 343:152-8. 2005
    ..After delipidation, GPI proteins were solubilized from the pellet using a mixture of organic solvent containing ethanol and water...
  32. ncbi [Study of the metal precipitation from decontamination leachates of municipal wastes fly ash incinerators]
    B Levasseur
    Institut national de la Recherche Scientifique Centre Eau, Terre et Environnement, Universite du Quebec, 490 rue de la Couronne, Quebec, QC, Canada, G1K 9A9
    Environ Technol 26:421-31. 2005
    ..The objective of this study was to evaluate different total and selective precipitation methods for metals removal from ash decontamination leachates...
  33. ncbi Aerobic sulfide production and cadmium precipitation by Escherichia coli expressing the Treponema denticola cysteine desulfhydrase gene
    C L Wang
    Department of Chemical Engineering, University of California, Berkeley 94720-1462, USA
    Appl Microbiol Biotechnol 56:425-30. 2001
    ..Without supplemental cysteine, both the E. coli producing cysteine desulfhydrase and the control E. coli demonstrated minimal cadmium removal...
  34. ncbi Are picoplankton responsible for calcite precipitation in lakes?
    Maria Dittrich
    Swiss Federal Institute for Environmental Science and Technology, Eawag, Switzerland
    Ambio 33:559-64. 2004
    ..It has been accepted that calcite precipitation can be induced by bacteria and algae. However, the detailed mechanisms are still unclear...
  35. doi Stability of equine IgG antivenoms obtained by caprylic acid precipitation: towards a liquid formulation stable at tropical room temperature
    Alvaro Segura
    Instituto Clodomiro Picado, Facultad de Microbiologia, Universidad de Costa Rica, San Jose, Costa Rica
    Toxicon 53:609-15. 2009
    ..Results suggest that formulation based on sorbitol is an option to obtain liquid whole IgG antivenoms of higher stability at tropical room temperatures...
  36. ncbi [Heparin-induced extracorporeal LDL precipitation (H.E.L.P.)]
    D Horstkotte
    Herz und Diabeteszentrum NRW Kardiologische Klinik, GeorgStr 11, 32545, Bas Oeynhausen, Germany
    Z Kardiol 92:III1-5. 2003
    The H.E.L.P procedure (heparin-induced extracorporeal LDL precipitation) is a selective and sophisticated apheresis procedure. By means of heparin and lowering the pH level, lipoproteins and fibrinogen are reduced by 50-60%...
  37. ncbi [Responses of three Stipa communities net primary productivity along Northeast China Transect to seasonal distribution of precipitation]
    Wenping Yuan
    Laboratory of Quantitative Vegetation Ecology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    Ying Yong Sheng Tai Xue Bao 16:605-9. 2005
    ..and based on an integral regression model, discussed the responses of the NPP to the seasonal distribution of precipitation, aimed to reveal the effects of precipitation availability on plant NPP...
  38. ncbi Preparation of immunoglobulin Y from egg yolk using ammonium sulfate precipitation and ion exchange chromatography
    K Y Ko
    Department of Animal Science, Iowa State University, Ames, Iowa 50011, USA
    Poult Sci 86:400-7. 2007
    ..The concentrated solution was further purified using either cation exchange chromatography or ammonium sulfate precipitation. For the cation exchange chromatography method, the concentrated sample was loaded onto a column equilibrated ..
  39. doi Effect of polyols on polyethylene glycol (PEG)-induced precipitation of proteins: Impact on solubility, stability and conformation
    Vineet Kumar
    Department of Pharmaceutical Sciences, University of Connecticut, Storrs, CT 06269, USA
    Int J Pharm 366:38-43. 2009
    ..Glucose, sucrose, raffinose, glycerol and sorbitol, all reduced the extent of protein precipitation. Solubility of BSA in the presence of ethylene glycol increased in the case of PEG 1450 and PEG 8000, but was ..
  40. doi Polyethylene glycol precipitation: proceed with care
    Michael Fahie-Wilson
    Ann Clin Biochem 45:233-5. 2008
  41. ncbi Nickel accumulation and nickel oxalate precipitation by Aspergillus niger
    A Magyarosy
    Department of Chemical Engineering, University of California, Berkeley 94720 1462, USA
    Appl Microbiol Biotechnol 59:382-8. 2002
    ..X-ray and electron diffraction analysis showed that these crystals were nickel oxalate dihydrate...
  42. pmc EQCM immunoassay for phosphorylated acetylcholinesterase as a biomarker for organophosphate exposures based on selective zirconia adsorption and enzyme-catalytic precipitation
    Hua Wang
    Pacific Norwest National Laboratory, Richland, WA 99352, United States
    Biosens Bioelectron 24:2377-83. 2009
    ..Moreover, 4-chloro-1-naphthol (CN) was studied as an ideal chromogenic substrate for the enzyme-catalytic precipitation. Experimental results show that the developed EQCM technique can allow for the detection of Phospho-AChE in ..
  43. ncbi Stabilizer choice for rapid dissolving high potency itraconazole particles formed by evaporative precipitation into aqueous solution
    Prapasri Sinswat
    College of Pharmacy, University of Texas at Austin, Austin, TX 78712, USA
    Int J Pharm 302:113-24. 2005
    ..including dissolution, of ultra-high potency powders containing itraconazole (ITZ) formed by evaporative precipitation into aqueous solution (EPAS)...
  44. ncbi Precipitation of lysozyme nanoparticles from dimethyl sulfoxide using carbon dioxide as antisolvent
    Gerhard Muhrer
    ETH Swiss Federal Institute of Technology Zurich, Institute of Process Engineering, Sonneggstrasse 3, CH 8092 Zurich, Switzerland
    Biotechnol Prog 19:549-56. 2003
    ..Moreover, these are consistent with the theoretical understanding of gas antisolvent recrystallization as achieved by using a recently developed model of the process...
  45. ncbi Supersaturation and renal precipitation: the key to stone formation?
    John P Kavanagh
    Department of Urology, South Manchester University Hospitals Trust, Wythenshawe Hospital, Manchester, UK
    Urol Res 34:81-5. 2006
  46. pmc Lead precipitation by Vibrio harveyi: evidence for novel quorum-sensing interactions
    Chad E Mire
    Department of Biological Sciences, Rowan University, Glassboro, New Jersey 08028, USA
    Appl Environ Microbiol 70:855-64. 2004
    ..200 degrees C. Regulation of the precipitation phenotype was also examined...
  47. ncbi Effects of solution conditions on the precipitation of phosphate for recovery. A thermodynamic evaluation
    Yonghui Song
    Institute for Aquatic Environmental Engineering, University of Karlsruhe, Germany
    Chemosphere 48:1029-34. 2002
    To understand the effects of solution conditions on the precipitation of calcium phosphates from wastewater for recovery, a computer programme PHREEQC was employed to calculate the speciation and saturation-index (SI) with respect to ..
  48. ncbi In-gel precipitation of enzymatically released phosphate
    Ana D Simonović
    Department of Biological Sciences, North Dakota State University, Fargo, ND 58105, USA
    Anal Biochem 334:312-7. 2004
    The phosphate precipitation reaction using ammonium molybdate and triethylamine under low pH has been applied to gel-based assays for detecting phosphate-releasing enzymes. The sensitivity of the assay is 10 pmol Pi/mm2 of 1...
  49. ncbi Preparation of All-Trans Retinoic Acid nanosuspensions using a modified precipitation method
    X Zhang
    State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory of Biomaterials and Devices, Southeast University, Nanjing, China
    Drug Dev Ind Pharm 32:857-63. 2006
    All-Trans Retinoic Acid (ATRA) nanosuspensions were prepared with a modified precipitation method. The ATRA solution in acetone was injected into pure water by an air compressor under the action of ultrasonication...
  50. pmc Evaluation of Western blotting methods using samples with or without sodium phosphotungstic acid precipitation for diagnosis of scrapie and chronic wasting disease
    Hongsheng Huang
    Ottawa Laboratory Fallowfield, Animal Diseases Research Institute, Canadian Food Inspection Agency, 3851 Fallowfield Road, Ottawa, Ontario
    Can J Vet Res 69:193-9. 2005
    ..procedures: an abnormal prion protein (PrPSc) concentration procedure using sodium phosphotungstic acid (PTA) precipitation and a procedure using crude sample without precipitation...
  51. ncbi Drug particle size influence on enteric beads produced by a droplet extrusion/precipitation method
    A M Cerdeira
    , , Portugal
    J Microencapsul 17:733-41. 2000
    The influence of drug particle size on the production of enteric beads by a polymer precipitation technique was investigated. Drug particle dimensions are known to play an important role in most microencapsulation techniques...
  52. pmc An in vitro study on the compatibility and precipitation of a combination of ciprofloxacin and vancomycin in human vitreous
    M Hui
    Department of Microbiology, The Chinese University of Hong Kong, Hong Kong, ROC
    Br J Ophthalmol 88:218-22. 2004
    To investigate the precipitation process of a mixture of vancomycin and ciprofloxacin by equilibrium dialysis and its subsequent effect on the level of available free antibiotics.
  53. ncbi Isolation and biochemical characterization of extracellular polymeric secretions (EPS) from modern soft marine stromatolites (Bahamas) and its inhibitory effect on CaCO3 precipitation
    Tomohiro Kawaguchi
    Department of Environmental Health Sciences, School of Public Health, University of South Carolina, Columbia 29208, USA
    Prep Biochem Biotechnol 32:51-63. 2002
    ..were conducted to examine biochemical characteristics and the role of EPS in the inhibition of CaCO3 precipitation. EPS consisted of acid polysaccharides and proteins...
  54. ncbi Surface-grafted viologen for precipitation of silver nanoparticles and their combined bactericidal activities
    Zhilong Shi
    Department of Chemical and Biomolecular Engineering, National University of Singapore, Kent Ridge, Singapore 119260
    Langmuir 20:6847-52. 2004
    ..The dual functionalities of HVVN and silver remain stable after prolonged immersion in phosphate buffer solution and after aging in a weathering chamber...
  55. ncbi Precipitation scavenging of polychlorinated biphenyls and polycyclic aromatic hydrocarbons along an urban to over-water transect
    John H Offenberg
    Chesapeake Biological Laboratory, The University of Maryland Center for Environmental Science, Solomons 20688, USA
    Environ Sci Technol 36:3763-71. 2002
    Concentrations of semi-volatile organic compounds in dissolved and particulate forms in precipitation are related to those measured concurrently in air measured at multiple locations along the tracks of several storms...
  56. ncbi Consolidation of degraded ornamental porous limestone stone by calcium carbonate precipitation induced by the microbiota inhabiting the stone
    C Jimenez-Lopez
    Dpto Microbiologia, Universidad de Granada, Fuentenueva s n, 18071 Granada, Spain
    Chemosphere 68:1929-36. 2007
    ..to activate, among the microbial community of the stone, those bacteria with a potential for calcium carbonate precipitation. In order to test this new method for stone consolidation, non-sterilized decayed porous limestone was ..
  57. ncbi High-sensitivity detection for model organophosphorus and carbamate pesticide with quartz crystal microbalance-precipitation sensor
    Namsoo Kim
    Korea Food Research Institute, San 46 1, Baekhyun dong, Bundang gu, Songnam si, Kyonggi do 463 746, Republic of Korea
    Biosens Bioelectron 22:1593-9. 2007
    ..coupling of AChE to 3-mercaptopropionic acid self-assembled monolayer, on the responses of a batch-type QCM-precipitation sensor was compared, resulting in a better sensitivity and binding efficiency in the former method...
  58. ncbi Linking chemical reactivity and protein precipitation to structural characteristics of foliar tannins
    T E C Kraus
    Department of Land, Air and Water Resources, University of California, One Shields Avenue, Davis, California 95616, USA
    J Chem Ecol 29:703-30. 2003
    ..growing in the pygmy forest of the northern California coast were examined for chemical reactivity, protein precipitation capacity (PPC), and structural characteristics (13C NMR)...
  59. ncbi Nephrocalcinosis: molecular insights into calcium precipitation within the kidney
    John A Sayer
    School of Cell and Molecular Biosciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    Clin Sci (Lond) 106:549-61. 2004
    ..Under these conditions, urine concentration and supersaturation leads to calcium crystal precipitation, which may be an intratubular event or initiate within the renal interstitium...
  60. ncbi Screening of antifungal agents using ethanol precipitation and bioautography of medicinal and food plants
    Gracilene Schmourlo
    Nucleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro UFRJ, Centro de Ciencias da Saude CCS, Bloco H, Cidade Universitaria, Ilha do Fundao, Rio de Janeiro, RJ 21941 590, Brazil
    J Ethnopharmacol 96:563-8. 2005
    In the search for bioactive compounds, bioautography and ethanol precipitation of macromolecules (proteins, polysaccharides, etc.) of plant aqueous extracts were associated in an antifungal screening...
  61. ncbi Highly Efficient Phosphopeptide Enrichment by Calcium Phosphate Precipitation Combined with Subsequent IMAC Enrichment
    Xumin Zhang
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense, Denmark
    Mol Cell Proteomics 6:2032-42. 2007
    ..The method is based on calcium phosphate precipitation of the phosphopeptides prior to further enrichment with established affinity enrichment methods...
  62. doi Effect of surface roughness of Ti, Zr, and TiZr on apatite precipitation from simulated body fluid
    Xiaobo Chen
    Centre for Material and Fibre Innovation, Deakin University, Pigdons Road, Waurn Ponds, Victoria 3217, Australia
    Biotechnol Bioeng 101:378-87. 2008
    ..6 microm led to the fastest apatite formation on the metal surfaces. This apatite layer on the metal surface is expected to bond to the surrounding bones directly after implantation...
  63. ncbi Clinical significance of small dense low-density lipoprotein cholesterol levels determined by the simple precipitation method
    Tsutomu Hirano
    First Department of Internal Medicine, Showa University School of Medicine, 1 5 8 Hatanodai, Shinagawa ku, Tokyo 142 8666, Japan
    Arterioscler Thromb Vasc Biol 24:558-63. 2004
    ..established a simple method for the quantification of small dense LDL cholesterol (C) using heparin-magnesium precipitation. The small dense LDL-C level was identical to cholesterol in the denser LDL fraction with a density of 1...
  64. pmc Combination of methylated-DNA precipitation and methylation-sensitive restriction enzymes (COMPARE-MS) for the rapid, sensitive and quantitative detection of DNA methylation
    Srinivasan Yegnasubramanian
    Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine 1650 Orleans Street, CRB 116, Baltimore, MD 21231, USA
    Nucleic Acids Res 34:e19. 2006
    ..This novel technology could significantly improve our ability to detect CGI hypermethylation...
  65. ncbi Metal chelate affinity precipitation of RNA and purification of plasmid DNA
    Sindhu Balan
    Department of Biology and Biochemistry, University of Houston, 4800 Calhoun, Houston, TX 77204 5001, USA
    Biotechnol Lett 25:1111-6. 2003
    ..Here we describe a metal affinity precipitation method to resolve RNA from linear and plasmid DNA...
  66. ncbi Fractionation of equine antivenom using caprylic acid precipitation in combination with cationic ion-exchange chromatography
    Rutai Raweerith
    Department of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Road, Rajthevi, Bangkok 10400, Thailand
    J Immunol Methods 282:63-72. 2003
    A combined process of caprylic acid (CA) precipitation and ion-exchange chromatography on SP-Sepharose was studied as a means to fractionate pepsin-digested horse antivenom F(ab')(2) antibody...
  67. ncbi Protein precipitation and denaturation by dimethyl sulfoxide
    Tsutomu Arakawa
    Alliance Protein Laboratories, Thousand Oaks, CA 91360, USA
    Biophys Chem 131:62-70. 2007
    ..an unfavorable interaction of DMSO with the native protein correlates with the enhanced self-association and precipitation of proteins by DMSO...
  68. pmc Phosphoproteomic analysis of human brain by calcium phosphate precipitation and mass spectrometry
    Qiangwei Xia
    Department of Human Genetics, The Center for Neurodegenerative Diseases, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Proteome Res 7:2845-51. 2008
    ..We used calcium phosphate precipitation to analyze an AD postmortem brain, followed by liquid chromatography-tandem mass spectrometry...
  69. ncbi Geographic variation in body size: the effects of ambient temperature and precipitation
    Yoram Yom-Tov
    Department of Zoology, Tel Aviv University, 69978, Tel Aviv, Israel
    Oecologia 148:213-8. 2006
    ..We used a conservative approach to discern the effects of precipitation and temperature by applying sequential regression...
  70. ncbi Supercritical fluid processing of proteins: lysozyme precipitation from aqueous solution
    Saeed Moshashaée
    Eli Lilly and Company, Indianapolis, IN 46285, USA
    J Pharm Pharmacol 55:185-92. 2003
    ..However, SEDS samples produced at 200 bar and 40 degrees C exhibited negligible disturbance. Thus the SEDS process utilising aqueous solution was able to bring about size reduction of lysozyme with minimal loss of biological activity...
  71. ncbi Controlled protein precipitation in combination with chip-based nanospray infusion mass spectrometry. An approach for metabolomics profiling of plasma
    K Olaf Boernsen
    Biomarker Development and Integrative Expression Profiling, Novartis Pharma AG, Basel, Switzerland
    Anal Chem 77:7255-64. 2005
    ..The feasibility of direct sample infusion using the new chip-based nanoelectrospray device opens a new dimension for the rapid parallel analysis of complex biological mixtures...
  72. pmc Microbial reduction and precipitation of vanadium by Shewanella oneidensis
    W Carpentier
    Laboratory for Protein Biochemistry and Protein Engineering, Flanders Interuniversity Institute for Biotechnology, B 9000 Ghent, Belgium
    Appl Environ Microbiol 69:3636-9. 2003
    ..The bacterium reduces V(V) (vanadate ion) to V(IV) (vanadyl ion) in an anaerobic atmosphere. The resulting vanadyl ion precipitates as a V(IV)-containing solid...
  73. ncbi Supercritical antisolvent precipitation of nimesulide: preliminary experiments
    M Moneghini
    Department of Pharmaceutical Sciences, University of Trieste, P le Europa 1, 34127 Trieste, Italy
    Curr Drug Deliv 4:241-8. 2007
    ..Finally, the stability of the nimesulide form II, checked by DSC analysis, was ruled on over a period of 15 months...
  74. ncbi Stimulation of microbial urea hydrolysis in groundwater to enhance calcite precipitation
    Yoshiko Fujita
    Idaho National Laboratory, Idaho Falls, Idaho 83415, USA
    Environ Sci Technol 42:3025-32. 2008
    ..Generation of carbonate alkalinity from ureolysis promotes calcite precipitation. In calcite-saturated aquifers, this represents a potential long-term contaminant sequestration mechanism...
  75. ncbi A new method for the isolation of histatins 1, 3, and 5 from parotid secretion using zinc precipitation
    B Flora
    Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, MA 02118 2392, USA
    Protein Expr Purif 23:198-206. 2001
    ..A fast and efficient two-step procedure was developed using zinc precipitation of histatins from human parotid secretion followed by final purification using reversed-phase high-performance ..
  76. ncbi Development and characterisation of a molecularly imprinted polymer prepared by precipitation polymerisation for the determination of phenylurea herbicides
    Rita Carabias-Martínez
    Departamento de Quimica Analitica, Nutricion y Bromatologia, Facultad de Quimica, Universidad de Salamanca, 37008 Salamanca, Spain
    J Sep Sci 28:453-61. 2005
    ..We observed that the imprinted polymers obtained by precipitation displayed a greater capacity to retain the phenylureas...
  77. pmc Urease induced calcium precipitation by Helicobacter species may initiate gallstone formation
    C Belzer
    Gut 55:1678-9. 2006
  78. ncbi Precipitation of octacalcium phosphates on artificial enamel in artificial saliva
    Y Sato
    School of Dentistry at Tokyo, Department of Preventive and Community Dentistry, The Nippon Dental University, Japan
    J Mater Sci Mater Med 17:1173-7. 2006
    ..The AE was placed in the AS at 37 degrees C. XRD, SEM and EDX investigated precipitation on the AE. The precipitation was always identified as being OCP...
  79. ncbi Ammonium hexafluorosilicate elicits calcium phosphate precipitation and shows continuous dentin tubule occlusion
    Toshiyuki Suge
    Department of Conservative Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School, 3 18 15 Kuramoto, Tokushima 770 8504, Japan
    Dent Mater 24:192-8. 2008
    ..However, AgF stains the teeth black due to silver precipitation. To overcome this drawback, the authors prepared ammonium hexafluorosilicate [SiF: (NH(4))(2)SiF(6)], which ..
  80. doi Formation and characterization of solid dispersions of piroxicam and polyvinylpyrrolidone using spray drying and precipitation with compressed antisolvent
    Ke Wu
    Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, USA
    J Pharm Sci 98:2422-31. 2009
    Solid dispersions of a poorly water-soluble drug piroxicam in polyvinylpyrrolidone (PVP) were prepared by precipitation with compressed antisolvent (PCA) and spray drying techniques...
  81. ncbi Rifampicin microparticles production by supercritical antisolvent precipitation
    Ernesto Reverchon
    Dipartimento di Ingegneria Chimica ed Alimentare, Universita di Salerno, Via Ponte Don Melillo, 84084 Fiscano SA, Italy
    Int J Pharm 243:83-91. 2002
    Semi-continuous supercritical antisolvent (SAS) precipitation has been used to produce Rifampicin micro- and nanoparticles with controlled particle size (PS) and particle size distribution (PSD)...
  82. ncbi Electrochemically assisted co-precipitation of protein with calcium phosphate coatings on titanium alloy
    Xiaoliang Cheng
    Department of Chemistry, Acadia University, 6 University Ave, Wolfville, Nova Scotia, Canada B4P 2R6
    Biomaterials 25:5395-403. 2004
    ..protein-containing calcium phosphate coating (BSA/brushite) was prepared by electrochemically assisted co-precipitation onto a hydroxyapatite (HA) coated Ti-6Al-4V surface...
  83. ncbi Extraction and precipitation of chitosan from cell wall of zygomycetes fungi by dilute sulfuric acid
    Akram Zamani
    School of Engineering, University of Borås, SE 50190 Borås, Sweden
    Biomacromolecules 8:3786-90. 2007
    ..It might indicate the inability of acetic acid to separate chitosan from the cell wall...
  84. ncbi Human vascular smooth muscle cells undergo vesicle-mediated calcification in response to changes in extracellular calcium and phosphate concentrations: a potential mechanism for accelerated vascular calcification in ESRD
    Joanne L Reynolds
    Department of Medicine, Addenbrooke s Hospital, Cambridge, United Kingdom
    J Am Soc Nephrol 15:2857-67. 2004
    ..under normal physiologic conditions did not calcify, and VSMC were also able to inhibit the spontaneous precipitation of Ca and P in solution...
  85. ncbi Quantitative validation of different protein precipitation methods in proteome analysis of blood platelets
    Maria Zellner
    Surgical Research Laboratories, Medical University of Vienna, Vienna, Austria
    Electrophoresis 26:2481-9. 2005
    For the preparation of proteins for proteome analysis, precipitation is frequently used to concentrate proteins and to remove interfering compounds...
  86. pmc Strain-specific ureolytic microbial calcium carbonate precipitation
    Frederik Hammes
    Laboratory of Microbial Ecology and Technology LabMET, Ghent University, B 9000 Ghent, Belgium
    Appl Environ Microbiol 69:4901-9. 2003
    During a study of ureolytic microbial calcium carbonate (CaCO(3)) precipitation by bacterial isolates collected from different environmental samples, morphological differences were observed in the large CaCO(3) crystal aggregates ..
  87. pmc Purported nanobacteria in human blood as calcium carbonate nanoparticles
    Jan Martel
    Department of Biochemistry and Cellular Molecular Biology, Chang Gung University, 259 Wen Hua First Road, Kwei Shan, Tao Yuan 333, Taiwan, Republic of China
    Proc Natl Acad Sci U S A 105:5549-54. 2008
    ..by introducing varying levels of CO(2) and NaHCO(3) as well as other conditions known to influence the precipitation of CaCO(3)...
  88. doi Plasma ascorbic acid preparation and storage for epidemiological studies using TCA precipitation
    Irma Salminen
    Department of Health and Functional Capacity, Biomarker Laboratory, National Public Health Institute, Helsinki, Finland
    Clin Biochem 41:723-7. 2008
    ..To introduce a procedure to validate an ascorbic acid method using trichloroacetic acid (TCA) for plasma stabilization at different storage temperatures...
  89. ncbi Differential influence of proteolysis by calpain 2 and Lp82 on in vitro precipitation of mouse lens crystallins
    Mitsuyoshi Azuma
    Research Laboratory, Senju Pharmaceutical Corporation Limited, Kobe 651 2241, Japan
    Biochem Biophys Res Commun 307:558-63. 2003
    ..Lp82-induced proteolysis of crystallins caused increased susceptibility of truncated crystallins to in vitro precipitation. Since Lp82 is highest in young animals, Lp82-induced proteolysis and precipitation may be one of the factors ..
  90. doi Silacidins: highly acidic phosphopeptides from diatom shells assist in silica precipitation in vitro
    Stephan Wenzl
    Lehrstuhl Biochemie I, Universitat Regensburg, 93040 Regensburg, Germany
    Angew Chem Int Ed Engl 47:1729-32. 2008
  91. ncbi A semi-automated 96-well protein precipitation method for the determination of montelukast in human plasma using high performance liquid chromatography/fluorescence detection
    Chester J Kitchen
    Merck Research Laboratories, WP75A 303, Sumneytown Pike, West Point, PA 19486, USA
    J Pharm Biomed Anal 31:647-54. 2003
    A simple, semi-automated, protein precipitation assay for the determination of montelukast (SINGULAIR, MK-0476) in human plasma has been developed...
  92. doi Synthesis by precipitation polymerisation of molecularly imprinted polymer microspheres for the selective extraction of carbamazepine and oxcarbazepine from human urine
    A Beltran
    Departament de Quimica Analitica i Quimica Organica, Universitat Rovira i Virgili, Tarragona, Spain
    J Chromatogr A 1216:2248-53. 2009
    ..imprinted polymers (MIPs), in the physical form of well-defined polymer microspheres, were synthesised via precipitation polymerisation (PP) using an antiepileptic drug, carbamazepine (CBZ), as template molecule, methacrylic acid ..
  93. ncbi Bacterial diversity and carbonate precipitation in the giant microbialites from the highly alkaline Lake Van, Turkey
    Purificación López-García
    Unité d Ecologie, Systematique et Evolution, UMR CNRS 8079, Universite Paris Sud, Orsay Cedex, France
    Extremophiles 9:263-74. 2005
    ..These heterotrophic bacteria may play a crucial role in the formation of Lake Van microbialites by locally promoting carbonate precipitation.
  94. ncbi CO2 and fluorinated solvent-based technologies for protein microparticle precipitation from aqueous solutions
    Marazban Sarkari
    Department of Chemical and Materials Engineering, University of Kentucky, Lexington, Kentucky 40506, USA
    Biotechnol Prog 19:448-54. 2003
    b>Precipitation with a compressed or supercritical fluid antisolvent (PCA) has been used to produce microparticles of biologically active proteins, pharmaceuticals, and polymers...
  95. ncbi Precipitation of mercuric sulfide nanoparticles in NOM-containing water: implications for the natural environment
    Amrika Deonarine
    Department of Civil and Environmental Engineering, Duke University, 121 Hudson Hall, Durham, North Carolina 27708, USA
    Environ Sci Technol 43:2368-73. 2009
    ..in surface waters and porewater of contaminated sediments as a result of kinetically hindered aggregation/precipitation reactions...
  96. ncbi Apparent solubility of hydroxyapatite in aqueous medium and its influence on the morphology of nanocrystallites with precipitation temperature
    K H Prakash
    School of Chemical and Biomedical Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798
    Langmuir 22:11002-8. 2006
    ..FESEM observations revealed that the aspect ratio of the particles decreased with increasing precipitation reaction temperature in one system [Ca(OH)2 + H3PO4] and in the other system it increased with increasing ..
  97. ncbi Precipitation of minerals by 22 species of moderately halophilic bacteria in artificial marine salts media: influence of salt concentration
    M A Rivadeneyra
    Department of Microbiology, University of Granada, 18071 Granada, Espana
    Folia Microbiol (Praha) 51:445-53. 2006
    b>Precipitation of minerals was shown by 22 species of moderately halophilic bacteria in both solid and liquid artificial marine salts media at different concentration and different Mg2+-to-Ca2+ ratio...
  98. ncbi Transfecting mammalian cells by DNA/calcium phosphate precipitates: effect of temperature and pH on precipitation
    E H Chowdhury
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Anal Biochem 314:316-8. 2003
  99. ncbi Effect of humic substances on the precipitation of calcium phosphate
    Yong Hui Song
    Chinese Research Academy of Environmental Sciences, Beijing 100012, China
    J Environ Sci (China) 18:852-7. 2006
    For phosphorus (P) recovery from wastewater, the effect of humic substances (HS) on the precipitation of calcium phosphate was studied...
  100. ncbi Corneal ciprofloxacin precipitation during bacterial keratitis
    Kirk R Wilhelmus
    Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA
    Am J Ophthalmol 136:1032-7. 2003
    ..To examine how age affects the risk of developing a white corneal precipitate during ciprofloxacin therapy for bacterial keratitis and to explore the effect of a white precipitate on rates of clinical improvement and cure...
  101. ncbi Calcium precipitation on the optical surfaces of a foldable intraocular lens: a clinicopathological correlation
    Suresh K Pandey
    Department of Ophthalmology, Storm Eye Institute, Medical University of South Carolina, 167 Ashley Ave, PO Box 250676, Charleston, SC 29425 5536, USA
    Arch Ophthalmol 120:391-3. 2002

Research Grants71

  1. Vitamin D and Estrogen Action in Bone: Concerted Role for hnRNPs in the C Family
    John S Adams; Fiscal Year: 2013
    ..Three specific aims are proposed: 1) using chromatin modification/precipitation, over/under expression and novel cell free translation technologies, explore the impact of hnRNPs on the cells ..
  2. Neuroprotection and Myelin Repair Mechanisms in Multiple Sclerosis
    Inderjit Singh; Fiscal Year: 2013
    ..served in World War II, the Korean conflict and the Gulf war in 1991 due to the environmental factors or the precipitation of the disease...
  3. ICSBP Function During Myeloid Differentiation
    ELIZABETH ANN EKLUND; Fiscal Year: 2011
    ..During the previous funding period, we pursued identification of such target-genes. Using chromatin immuno-precipitation and CpG island microarray screening, we identified ICSBP- target-genes which encode proteins involved in ..
  4. PTEN TUMOR SUPPRESSOR AND SIGNAL TRANSDUCTION
    RAMON E PARSONS; Fiscal Year: 2012
    ..A combination of overexpression, RNAi, and chromatin immuno- precipitation experiments are planned to identify the mechanism of activation...
  5. Impact of smoking on immune response and arthritis in humanized mice
    Veena Taneja; Fiscal Year: 2011
    ..inheriting DR4 develop arthritis suggesting other genetic and environmental factors that may be involved in precipitation of disease...
  6. Role of NPC1L1 in regulating energy metabolism, nutrient utilization & weight gai
    Philip N Howles; Fiscal Year: 2010
    ..unabsorbed cholesterol inhibits solubilization of saturated fatty acids by bile salt micelles causing their precipitation and excretion...
  7. ANALYSIS OF CHROMATIN REMODELING IN MAMMARY GLAND DEVELOPMENT USING TILING ARRAYS
    Monique Rijnkels; Fiscal Year: 2009
    ..problem is now possible with the recent development of ChIP-chip, a new technology combining Chromatin Immuno-precipitation (ChIP) and high-throughput oligonucleotide-tiling arrays (chip)...
  8. Transscleral Transport & Polymeric Delivery of Antibody & Steroids for Macular Ed
    Ashim K Mitra; Fiscal Year: 2010
    ..Risk of drug precipitation due to poor solubility;and short vitreal half life upon intravitreal injection are the key deterrents in their ..
  9. Study on the intracellular Network of TBX3
    Taosheng Huang; Fiscal Year: 2010
    ..The CHIP-GLAS assay combines chromatin precipitation and microarray, which could be used to identify 20,000 promoter DNA/transcription factor interactions in one ..
  10. The role of the nucleoporin Nup98 in gene regulation
    Martin W Hetzer; Fiscal Year: 2013
    ..For instance, the consequences of Nup98 knockdown and over-expression will be analyzed by chromatin immune- precipitation to investigate which binding partners, histone modifications and RNA polymerase II phosphorylation events ..
  11. Viral suppression of CNS autoimmunity
    Stephen A Stohlman; Fiscal Year: 2013
    ..infectious agents, are thought to be major contributing factors in both the induction of autoimmunity and in precipitation of relapse events...
  12. Germ cell specifications and differentiation in planarians.
    Phillip A Newmark; Fiscal Year: 2013
    ..Work performed under the first aim will use chromatin immuno-precipitation, next-generation sequencing, and computational approaches to identify targets of a germ cell-specific ..
  13. Plasma RNA based Early Lung Cancer Detection by Tethered Cationic Lipoplex Assay
    SERGE P NANA-SINKAM; Fiscal Year: 2013
    ..chain reaction (qRT-PCR) with pre-concentration techniques such as ultra-centrifugation or ExoQuickTM exosome precipitation kit (SBI Inc.), this approach is tedious, expensive and time consuming...
  14. Understanding the malaria-poverty vicious circle
    MARCIA CALDAS DE CASTRO; Fiscal Year: 2010
    ..Instruments for estimating the effect of malaria on SES include pregnancy status, elevation, and precipitation;instruments for estimating the effect of SES on malaria include gender and ethnicity;distance to major towns;..
  15. Structural and Functional Studies of the Novel DBD Domain of MKP5
    Amjad Farooq; Fiscal Year: 2012
    ..consequences of MKP5 DBD domain interaction with AP1 site in DNA will be analyzed using chromatin immuno-precipitation and luciferase reporter gene assays...
  16. GENETIC AND MOLECULAR ANALYSIS OF YEAST DNA REPLICATION
    Robert A Sclafani; Fiscal Year: 2012
    ..A combination of mutational analysis, whole-genome genetic screens, ChIP (chromatin immuno-precipitation), and affinity/antibody isolation will be used to test many of these hypotheses...
  17. Innate Immunity: Signal Transduction and Interferon Gene Regulation
    THOMAS P MANIATIS; Fiscal Year: 2010
    ..Cluster-wide changes in chromatin structure will be detected using chromatin precipitation (ChIP) and DNA microarray methods (chip)...
  18. Quick-Set Endodontic Material
    CAROLYN MARGARET PRIMUS; Fiscal Year: 2010
    ..will be tested by soaking prepared teeth in phosphate buffered saline solutions to observe the in situ precipitation of hydroxylapatite. The clinical handling and filling of roots and dentinal tubules will also be tested...
  19. Regulation of DNA Metabolism During Herpes Simplex Virus-1 Infection
    KEVIN FRANCIS BRYANT; Fiscal Year: 2010
    ..whether these complexes regulate the chromatin composition of the viral genome by performing chromatin immuno-precipitation assays...
  20. The Role of the Human Holliday Junction Resolvase Component SLX4 in DNA Recombina
    MOHAMED FIRAZ MOHIDEEN; Fiscal Year: 2013
    ..This will be tested by depleting SLX4 by siRNA coupled with co-precipitation and fluorescence-based localization experiments of MUS81 with the telomere-binding protein, TRF2...
  21. ALIMENTARY TRACT LIPIDS IN HEALTH AND DISEASE
    MARTIN CONRAD CAREY; Fiscal Year: 2013
    ..hepatic bile, where endogenous 13-glucuronidase hydrolysis of bilirubin glucuronides and calcium bilirubinate precipitation occurs...
  22. Optimization of the FPI model for epilepsy therapy development
    RAIMONDO D'AMBROSIO; Fiscal Year: 2013
    ..models employed in preclinical AED screening because they may not capture the mechanisms most important for precipitation and control of pharmacoresistant chronic spontaneous recurrent seizures (CSRSs) in humans...
  23. Effects of Climate Change on Cholera Dynamics and Prediction
    Shafiqul Islam; Fiscal Year: 2010
    ..Thus, a warmer global climate with increasingly variable precipitation patterns and accelerated sea level rise is likely to have wide-ranging effects on the outbreaks and ..
  24. Molecular Mechanisms of Cell Fate Decisions in Hair Follicle Stem Cells
    Tudorita Tumbar; Fiscal Year: 2013
    ..the distribution of histone marks across the genome at two stages of stem cell activity by chromatin immuno-precipitation and sequencing, and attempt to examine a potential link between two known adult tissue stem cells features: ..
  25. Neurobiological Correlates of Stress and Norepinephrine Transporter
    Meng Yang Zhu; Fiscal Year: 2012
    ..findings suggest that stress-induced alterations of NET may serve as the point of entry for stress-triggered precipitation of depression...
  26. Environmental Estrogen Induced Epigenetic Alteration of Uterine Stem Cells
    Hugh S Taylor; Fiscal Year: 2013
    ..The specific aims include 1) the use of chromatin precipitation, massively parallel sequencing and bisulfite sequencing to determine if exposure leads to preferential ..
  27. Molecular Evolution of Multifunctional DNA Nanoparticles
    Bradley T Messmer; Fiscal Year: 2013
    ..Two approaches will be used. In the first, we will perform co- precipitation experiments after crosslinking biotinylated DNA nanoparticles to the cell surface molecules...
  28. The Cyto-Protective Role of Hexokinase II During Metabolic Stress
    JONATHAN MICHAEL GALL; Fiscal Year: 2012
    ..Protein-protein interactions and HKII ubiquitination will be studied by immuno-precipitation. Mitchondrial fission will be evaluated by confocal microscopy using a mitochondrial dye and mitochondrial ..
  29. Hypothalamic Neuronal Plasticity in Chronic Stress
    De Pei Li; Fiscal Year: 2013
    ..The chronic unpredictable mild stress (CUMS) rat model provides a good analogy to the precipitation of depression by chronic and low-grade stressors in humans...
  30. Protocadherin Gene Expression and Function
    THOMAS P MANIATIS; Fiscal Year: 2012
    ..identification of the proteins that bind specifically to these sequences through whole genome Chromatin Immuno-Precipitation and deep sequencing (ChIP-Seq) experiments...
  31. Overwintering mortality of nymphal Ixodes scapularis, Lyme disease vector, in a c
    RICHARD S contact OSTFELD; Fiscal Year: 2010
    ..by applicant): Anthropogenic climate change is causing rising global temperatures, changes in patterns of precipitation, and, according to many, an increase in the extent and impact of vector-borne infectious disease on human ..
  32. Analgesics targeting truncated 6transmembrane exon 11 variants of MOR-1 (6TM-E11)
    Susruta Majumdar; Fiscal Year: 2013
    ..to morphine and can be given to morphine-dependent mice without a decrease in its own analgesic actions or the precipitation of withdrawal...
  33. Rapid genome-wide mapping of mammalian meiotic recombination hotspots.
    PHILIPPE ROGER JEAN BOIS; Fiscal Year: 2010
    ..together with our unique expertise in purifying meiotic cells by FACS combined with chromatin immuno-precipitation (ChIP), we are aiming to generate DSB "recombinome" atlases in mice by targeting DNA bound to the Dmc1 protein,..
  34. Selective Protein Capture by Epitope Imprinting
    Kenneth J Shea; Fiscal Year: 2012
    ..Two methods for the preparation of MIP nanoparticles are being developed, precipitation polymerization and suspension polymerization...
  35. Mitochondrial calcium homeostasis in SOD1-familial ALS
    Giovanni Manfredi; Fiscal Year: 2013
    ..the ER- CyPD interactions with SOD1, by mitochondrial fractionation, immuno-electron microscopy, and immuno-precipitation. 3) Test different complementary approaches to prevent Ca2+ overload in FALS mitochondria and improve disease ..
  36. Role of microRNAs in regulation of the marrow microenvironment
    Manoj M Pillai; Fiscal Year: 2013
    ..This technique, termed HITS-CLIP (High Through-put Sequencing following Cross-Linked Immuno Precipitation) utilizes ultraviolet (UV) radiation to cross-link miRs and mRNAs to the protein Argonaute, a component of the ..
  37. Poxvirus-mediated change to proteasomal peptide signatures in macrophages
    Dorota Skowyra; Fiscal Year: 2013
    ..western blot and immuno-cytochemistry to detect expression of 26S and 26Si specific subunits;immuno-precipitation with affinity purified, subunit-specific antibodies to characterize and isolate 26S ad 26Si proteasomes;..
  38. Regulation of histone H3 dephosphorylation in breast cancer cells
    PEDRO RODRIGUEZ COLLAZO RODRIGUEZ-COLLAZO; Fiscal Year: 2011
    ..Use cell immunofluorescence, Western blot, gel electrophoresis, chromatin immuno- precipitation, co-immuno-precipitation to elucidate phosphatosome physiological role...
  39. Mechanisms of uremic vascular calcification
    Koba A Lomashvili; Fiscal Year: 2013
    ..be solely the result of circulating calcium and phosphate concentrations that exceed the levels of spontaneous precipitation but it is now clear that vascular smooth muscle is an active participant in this process...
  40. Molecular mechanisms of Ca2+ retention in mitochondria
    Anatoly Starkov; Fiscal Year: 2010
    ..There is currently no explanation to this fact. A precipitation of Ca2+ and phosphate in the mitochondrial matrix has been suggested to be the form of storage of accumulated ..
  41. Regulation of anti-endocrine resistance of breast cancer by a network of non-codi
    Peter Kabos; Fiscal Year: 2013
    ..miRs and mRNAs to the argonaute proteins followed by the isolation of RNA- protein complexes by immune precipitation, isolation and sequencing of the RNA...
  42. Structural Determination of Full-length APOBEC3G and the APOBEC3G/Vif Complex
    TROY CHRISTOPHER KRZYSIAK; Fiscal Year: 2011
    ..specific interactions leading to soluble complexes and and minimize the nonspecific interactions that lead to precipitation. Also, the ability of APOBEC3GA/if complexes to bind important factors for the incorporation of APOBEC3G into ..
  43. Characterization of ER-localized Activity of HHV-8 Interleukin-6
    EMILY MARIE COUSINS; Fiscal Year: 2013
    ..In a yeast two-hybrid screen and subsequently by co-precipitation assay, VKORC1v2 was also shown to interact with thioredoxin-like protein 1 (TMX1), an ER-localized protein ..
  44. Air Barrier System to reduce contamination of wounds during surgery
    SEAN SELF; Fiscal Year: 2013
    ..The ability to prevent airborne precipitation of bacteria into the surgical site is an important factor in reducing postoperative infection, particularly in ..
  45. Linked Virulence and Fitness of Mycoplasma alligatoris
    Daniel R Brown; Fiscal Year: 2010
    ..Assessing the importance of genome pre-adaptation with modestly advantageous genes like hyaluronidase to precipitation of severe virulence following horizontal transfer of a system like nan could lead to predictive models for the ..
  46. Osteogenic Calcium Phosphate Nanoparticles with Designable Drug Release Kinetics
    Vuk Uskoković; Fiscal Year: 2012
    ..a) Synthesis of multifunctional therapeutic and osteoinductive calcium phosphate nanoparticles by means of co-precipitation from ultrasonically agitated emulsions;b) Drug release studies in bacterial and cell cultures...
  47. Multiplex Fluorescent Dyes for Ultrasensitive Proteomics
    Edward A Dratz; Fiscal Year: 2010
    ..prepare and test highly water soluble Zdye saturation labels that react with protein thiols to avoid precipitation of labeled proteins (which is a problem with available thiol saturation labels for 2D gel detection), to ..
  48. Genetic characterization of phytochrome nuclear bodies in plant light signaling
    Meng Chen; Fiscal Year: 2013
    ..test this hypothesis, we propose to identify global phytochrome NB associated chromosome loci using chromatin- precipitation followed by deep sequencing (ChIP-seq), to determine the relationship between gene positioning to phytochrome ..
  49. Mechanism of transcript elongation control by RfaH
    Irina Artsimovitch; Fiscal Year: 2013
    ..the RfaH regulon by identifying the RfaH-associated proteins and genes by in vivo crosslinking and chromatin immuno-precipitation, respectively. We will also analyze selected RfaH operons by quantitative RT PCR.
  50. Serum Glyco-Markers of Early Hepatocellular Carcinoma Using a Mass Spec Approach
    David M Lubman; Fiscal Year: 2013
    ..In this method, immunoaffinity precipitation is used to extract a target glycoprotein from serum, the glycans removed and then the glycans are profiled ..
  51. Climate Variability, Migration and Intergenerational Transfers
    Leah K VanWey; Fiscal Year: 2012
    ..These data will then be merged with precipitation measures constructed from hourly rainfall data from approximately the last 35 years from 256 weather stations ..
  52. Fluorescence Intensity-Based Scoring of Macromolecule Crystallization Plates
    MARC LEE PUSEY; Fiscal Year: 2010
    ..5% of the molecules labeled with fluorescent probe), and the graduated scoring of precipitation outcomes that reflects the experimentally determined propensity of those outcomes to be optimized to ..
  53. Antigen identification for B cell response in multiple sclerosis lesions
    Mark P Burgoon; Fiscal Year: 2013
    ..lesions of relapsing/remitting MS brains, using state-of-the-art immunologic techniques that include staining, precipitation and immunopanning techniques developed in this lab...
  54. Virtual Gating Machines: A new technology for protein purification
    Michael P Rout; Fiscal Year: 2013
    ..the NPC can be used to purify any protein, offering potential alternatives to chromatography, filtration and precipitation as protein purification techniques to the biotechnology industry...
  55. Generation of an In Vivo Human Genome Enhancer Dataset
    LEN ALEXANDER PENNACCHIO; Fiscal Year: 2013
    ..More recently, we demonstrated the power of chromatin immuno- precipitation targeting an enhancer-associated transcriptional coactivator (p300) coupled with massively parallel next ..
  56. An Intregated System for the Epidemiological Application of Earth Observation Tec
    Michael C Wimberly; Fiscal Year: 2011
    ..This system will integrate a variety of currently-available datasets including land surface temperature, precipitation, vegetation indices, and land cover maps...
  57. Crystallin-Derived Anti-Chaperones in the Lens
    Krishna K Sharma; Fiscal Year: 2013
    ..crystallin fragments generate H2O2, display anti-chaperone activity and induce lens protein aggregation and precipitation. Studies will be performed in both human lenses and in the guinea pig model of age-related cataractogenesis...
  58. MECHANISMS OF AIRFLOW OBSTRUCTION
    Julian Solway; Fiscal Year: 1992
    ..While these have provided insight into the precipitation of airways response, the detailed sequence of events stemming from conditioning of inspired air to airflow ..
  59. Proteinase Inhibitors and Crystallin Fragments in Cataract
    OM PRAKASH SRIVASTAVA; Fiscal Year: 2013
    ..by applicant): Age-related (senile) cataract is principally a crystallin protein aggregation and subsequent precipitation disease that occurs over the time frame of several years...
  60. Impact of climate change on mosquito-borne arbovirus transmission
    C M Barker; Fiscal Year: 2011
    ..for California vary, there is a general consensus that temperatures during the current century will rise, precipitation amount and timing will change, and ecosystem dynamics will be altered...
  61. Using deep-cavity cavitands to study supramolecular chemistry in water
    Bruce C Gibb; Fiscal Year: 2013
    ..varies as a function of co-solute salts, in a manner paralleling the ability of salts to induce either precipitation or solubilization of proteins...
  62. Developing a state-level health impact assessment of climate change in Wisconsin
    Henry A Anderson; Fiscal Year: 2011
    ..goal of this research project is to develop predictive models of both current and climate change-related precipitation variability that poses environmental health risks at a statewide scale required for public health preparedness...
  63. PHYSICAL CHEMISTRY OF BILIRUBIN & ITS CONJUGATES
    WILLIAM SPIVAK; Fiscal Year: 1990
    ..also use human and animal biles to determine in more detail what factors affect bile pigment hydrolysis and precipitation. We will continue our previous viscometric studies to determine the effect of changes in bile pigment and ..
  64. PLATELET SUFACE ACTIVATION IN HEMOSTASIS AND THROMBOSIS
    SANFORD SHATTIL; Fiscal Year: 1993
    ..A series of chemical cross-linking and immuno- precipitation strategies will also be employed to identify specific regulatory molecules that interact directly with the ..
  65. Peptides surface modification to prevent stent blockage
    Joseph Leung; Fiscal Year: 2006
    ..as a result of deconjugation of bilirubin diglucuronide by bacterial Beta-glucuronidase, resulting in the precipitation of calcium bilirubinate. This is attributed to enzymatic activities of aerobic bacteria such as E...
  66. CARBONATOAPATITE FORMATION IN ENAMEL MINERALIZATION
    Takaaki Aoba; Fiscal Year: 1993
    ..the carbonatoapatite in the enamel tissue at various developmental stages; b) assessing the driving force for precipitation of the enamel mineral during amelogenesis; and, as a long-term objective, c) investigating possible ..
  67. Silicone-free glass pre-filled syringe system for Biological Drugs.
    VINAY SAKHRANI; Fiscal Year: 2009
    ..Protein precipitation (UV/Vis spectroscopy), loss of soluble protein (SE-HPLC), influence of adsorbed protein on syringe forces, and ..
  68. BACTERIAL UREASE IN UTI
    HARRY MOBLEY; Fiscal Year: 2000
    ..mirabilis and is hydrolyzed by this enzyme. NH3, a product of hydrolysis, elevates urine pH causing precipitation of normally soluble ions forming struvite (MgNH4PO4) or carbonate-apatite (Ca10(PO4)6.CO3) stones...
  69. ENDOTHELIAL CELL AUTOIMMUNITY IN DIABETIC RETINOPATHY
    Ramesh Nayak; Fiscal Year: 2001
    ..We have three specific aims. The first seeks to identify endothelial cell auto-antigens by immuno-precipitation and N-terminal sequencing and also by the candidate antigen approach...
  70. RETROVIRAL GENE TRANSFER INTO PRIMARY ASTROCYTE
    MARION SHORT; Fiscal Year: 1992
    ..Levels of mature B-NGF will be determined by a two-site- radioimmunoassay and by immune precipitation using monoclonal and polyclonal antibodies to mouse B-NGF...
  71. NORMAL & PATHOLOGICAL ENAMEL MINERALIZATION
    Takaaki Aoba; Fiscal Year: 1993
    ..for understanding normal and pathological enamel mineralization is to determine: a) the driving force for precipitation of calcium phosphate salts during amelogenesis, b) the cellular role in establishing such a driving force, and ..