permeability

Summary

Summary: Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions.

Top Publications

  1. ncbi Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings
    C A Lipinski
    Central Research Division, Pfizer Inc, Groton, CT 06340, USA
    Adv Drug Deliv Rev 46:3-26. 2001
  2. ncbi Multifunctional strands in tight junctions
    S Tsukita
    Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    Nat Rev Mol Cell Biol 2:285-93. 2001
  3. pmc Mitofusin-2 maintains mitochondrial structure and contributes to stress-induced permeability transition in cardiac myocytes
    Kyriakos N Papanicolaou
    Molecular Cardiology Whitaker Cardiovascular Institute, Boston University School of Medicine, 715 Albany Street, W611, Boston, MA 02118, USA
    Mol Cell Biol 31:1309-28. 2011
  4. pmc Heterogeneous blood-tumor barrier permeability determines drug efficacy in experimental brain metastases of breast cancer
    Paul R Lockman
    Department of Pharmaceutical Sciences, Cancer Biology Center, Texas Tech University Health Sciences Center, Amarillo, Texas 79106, USA
    Clin Cancer Res 16:5664-78. 2010
  5. ncbi Mitochondrial membrane permeabilization in cell death
    Guido Kroemer
    Institut Gustave Roussy, Institut National de la Santé et de la Recherche Médicale Unit Apoptosis, Cancer and Immunity, Universite de Paris Sud XI, Villejuif, France
    Physiol Rev 87:99-163. 2007
  6. doi Blood-brain barrier tight junction permeability and ischemic stroke
    Karin E Sandoval
    Department of Pharmaceutical Sciences, School of Pharmacy, Southern Illinois University Edwardsville, Edwardsville, IL 62026, USA
    Neurobiol Dis 32:200-19. 2008
  7. pmc Increased gut permeability and microbiota change associate with mesenteric fat inflammation and metabolic dysfunction in diet-induced obese mice
    Yan Y Lam
    Boden Institute of Obesity, Nutrition, Exercise and Eating Disorders, University of Sydney, Sydney, Australia
    PLoS ONE 7:e34233. 2012
  8. doi The permeability of reconstituted nuclear pores provides direct evidence for the selective phase model
    Bastian B Hülsmann
    Abteilung Zelluläre Logistik, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077 Gottingen, Germany
    Cell 150:738-51. 2012
  9. ncbi The mitochondrial permeability transition pore and its involvement in cell death and in disease pathogenesis
    Andrea Rasola
    CNR Institute of Neuroscience and Department of Biomedical Sciences, University of Padova, Viale Giuseppe Colombo 3, I 35121 Padua, Italy
    Apoptosis 12:815-33. 2007
  10. pmc Changes in gut microbiota control inflammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability
    P D Cani
    Unit of Pharmacokinetics, Metabolism, Nutrition and Toxicology, Louvain Drug Research Institute, Universite Catholique de Louvain, Brussels, Belgium
    Gut 58:1091-103. 2009

Detail Information

Publications352 found, 100 shown here

  1. ncbi Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings
    C A Lipinski
    Central Research Division, Pfizer Inc, Groton, CT 06340, USA
    Adv Drug Deliv Rev 46:3-26. 2001
    Experimental and computational approaches to estimate solubility and permeability in discovery and development settings are described...
  2. ncbi Multifunctional strands in tight junctions
    S Tsukita
    Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    Nat Rev Mol Cell Biol 2:285-93. 2001
    ..New insights into the molecular architecture of tight junctions allow us to now discuss the structure and functions of this unique cell-cell adhesion apparatus in molecular terms...
  3. pmc Mitofusin-2 maintains mitochondrial structure and contributes to stress-induced permeability transition in cardiac myocytes
    Kyriakos N Papanicolaou
    Molecular Cardiology Whitaker Cardiovascular Institute, Boston University School of Medicine, 715 Albany Street, W611, Boston, MA 02118, USA
    Mol Cell Biol 31:1309-28. 2011
    ..The absence of Mfn-2 is associated with a marked delay in mitochondrial permeability transition downstream of Ca(2+) stimulation or due to local generation of reactive oxygen species (ROS)...
  4. pmc Heterogeneous blood-tumor barrier permeability determines drug efficacy in experimental brain metastases of breast cancer
    Paul R Lockman
    Department of Pharmaceutical Sciences, Cancer Biology Center, Texas Tech University Health Sciences Center, Amarillo, Texas 79106, USA
    Clin Cancer Res 16:5664-78. 2010
    ..Herein, we answer this question by measuring BTB passive integrity, chemotherapeutic drug uptake, and anticancer efficacy in vivo in two breast cancer models that metastasize preferentially to brain...
  5. ncbi Mitochondrial membrane permeabilization in cell death
    Guido Kroemer
    Institut Gustave Roussy, Institut National de la Santé et de la Recherche Médicale Unit Apoptosis, Cancer and Immunity, Universite de Paris Sud XI, Villejuif, France
    Physiol Rev 87:99-163. 2007
    ..The inhibition of MMP constitutes an important strategy for the pharmaceutical prevention of unwarranted cell death. Conversely, induction of MMP in tumor cells constitutes the goal of anticancer chemotherapy...
  6. doi Blood-brain barrier tight junction permeability and ischemic stroke
    Karin E Sandoval
    Department of Pharmaceutical Sciences, School of Pharmacy, Southern Illinois University Edwardsville, Edwardsville, IL 62026, USA
    Neurobiol Dis 32:200-19. 2008
    ..Under ischemic stroke conditions decreased BBB TJ integrity results in increased paracellular permeability, directly contributing to cerebral vasogenic edema, hemorrhagic transformation, and increased mortality...
  7. pmc Increased gut permeability and microbiota change associate with mesenteric fat inflammation and metabolic dysfunction in diet-induced obese mice
    Yan Y Lam
    Boden Institute of Obesity, Nutrition, Exercise and Eating Disorders, University of Sydney, Sydney, Australia
    PLoS ONE 7:e34233. 2012
    ..The current results implicate gut dysfunction, and attendant inflammation of contiguous adipose, as salient features of the metabolic dysregulation of diet-induced obesity...
  8. doi The permeability of reconstituted nuclear pores provides direct evidence for the selective phase model
    Bastian B Hülsmann
    Abteilung Zelluläre Logistik, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077 Gottingen, Germany
    Cell 150:738-51. 2012
    Nuclear pore complexes (NPCs) maintain a permeability barrier between the nucleus and the cytoplasm through FG-repeat-containing nucleoporins (Nups)...
  9. ncbi The mitochondrial permeability transition pore and its involvement in cell death and in disease pathogenesis
    Andrea Rasola
    CNR Institute of Neuroscience and Department of Biomedical Sciences, University of Padova, Viale Giuseppe Colombo 3, I 35121 Padua, Italy
    Apoptosis 12:815-33. 2007
    Current research on the mitochondrial permeability transition pore (PTP) and its role in cell death faces a paradox...
  10. pmc Changes in gut microbiota control inflammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability
    P D Cani
    Unit of Pharmacokinetics, Metabolism, Nutrition and Toxicology, Louvain Drug Research Institute, Universite Catholique de Louvain, Brussels, Belgium
    Gut 58:1091-103. 2009
    Obese and diabetic mice display enhanced intestinal permeability and metabolic endotoxaemia that participate in the occurrence of metabolic disorders. Our recent data support the idea that a selective increase of Bifidobacterium spp...
  11. ncbi Endoplasmic reticulum stress induces calcium-dependent permeability transition, mitochondrial outer membrane permeabilization and apoptosis
    A Deniaud
    Universite de Versailles SQY, CNRS UMR 8159, 45, Versailles, France
    Oncogene 27:285-99. 2008
    ..the mitochondrial matrix induced by ER stress triggered signs of proapoptotic mitochondrial alteration, namely permeability transition, dissipation of the electrochemical potential, matrix swelling, relocalization of Bax to ..
  12. ncbi Regulation of tight junction permeability and occludin phosphorylation by Rhoa-p160ROCK-dependent and -independent mechanisms
    T Hirase
    First Department of Internal Medicine, Kobe University School of Medicine, 7 5 2 Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
    J Biol Chem 276:10423-31. 2001
    In epithelial and endothelial cells, tight junctions regulate the paracellular permeability of ions and proteins...
  13. pmc A size-exclusion permeability barrier and nucleoporins characterize a ciliary pore complex that regulates transport into cilia
    Hooi Lynn Kee
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Nat Cell Biol 14:431-7. 2012
    ..Together, this work demonstrates that the physical and molecular nature of the ciliary pore complex is similar to that of the nuclear pore complex, and further extends functional parallels between nuclear and ciliary import...
  14. pmc Cyclophilin D is a component of mitochondrial permeability transition and mediates neuronal cell death after focal cerebral ischemia
    Anna C Schinzel
    Howard Hughes Medical Institute and Dana Farber Cancer Institute, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:12005-10. 2005
    Mitochondrial permeability transition (PT) is a phenomenon induced by high levels of matrix calcium and is characterized by the opening of the PT pore (PTP)...
  15. ncbi Properties of the permeability transition pore in mitochondria devoid of Cyclophilin D
    Emy Basso
    Department of Biomedical Sciences and Consiglio Nazionale delle Ricerche Institute of Neuroscience, University of Padova, Viale Giuseppe Colombo 3, I 35121 Padova, Italy
    J Biol Chem 280:18558-61. 2005
    We have studied the properties of the permeability transition pore (PTP) in mitochondria from the liver of mice where the Ppif gene encoding for mitochondrial Cyclophilin D (CyP-D) had been inactivated...
  16. pmc TNF-α signals through PKCζ/NF-κB to alter the tight junction complex and increase retinal endothelial cell permeability
    Célia A Aveleira
    Centre of Ophthalmology and Vision Sciences, Institute of Biomedical Research in Light and Image, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
    Diabetes 59:2872-82. 2010
    ..in the vitreous of diabetic patients and in retinas of diabetic rats associated with increased retinal vascular permeability. However, the molecular mechanisms underlying retinal vascular permeability induced by these cytokines are ..
  17. ncbi Exploiting the enhanced permeability and retention effect for tumor targeting
    Arun K Iyer
    Laboratory of Microbiology and Oncology, Faculty of Pharmaceutical Sciences, Sojo University, Ikeda 862 0082, Japan
    Drug Discov Today 11:812-8. 2006
    Of the tumor targeting strategies, the enhanced permeability and retention (EPR) effect of macromolecules is a key mechanism for solid tumor targeting, and considered a gold standard for novel drug design...
  18. ncbi Mitochondrial transport of cations: channels, exchangers, and permeability transition
    P Bernardi
    Department of Biomedical Sciences, University of Padova, and Consiglio Nazionale delle Ricerche Center for the Study of Biomembranes, Padova, Italy
    Physiol Rev 79:1127-55. 1999
    ....
  19. ncbi Increased intestinal permeability in obese mice: new evidence in the pathogenesis of nonalcoholic steatohepatitis
    Paola Brun
    Department of Histology, Univ of Padua, Via Gabelli 63, Padova, Italy
    Am J Physiol Gastrointest Liver Physiol 292:G518-25. 2007
    ..In conclusion, genetically obese mice display enhanced intestinal permeability leading to increased portal endotoxemia that makes HSCs more sensitive to bacterial endotoxins...
  20. pmc BID, BIM, and PUMA are essential for activation of the BAX- and BAK-dependent cell death program
    Decheng Ren
    Molecular Oncology, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Science 330:1390-3. 2010
    ..Thus, many forms of apoptosis require direct activation of BAX and BAK at the mitochondria by a member of the BID, BIM, or PUMA family of proteins...
  21. ncbi Bax and adenine nucleotide translocator cooperate in the mitochondrial control of apoptosis
    I Marzo
    CNRS, UPR 420, 19 rue Guy Moquet, F 94801 Villejuif, France
    Science 281:2027-31. 1998
    The proapoptotic Bax protein induces cell death by acting on mitochondria. Bax binds to the permeability transition pore complex (PTPC), a composite proteaceous channel that is involved in the regulation of mitochondrial membrane ..
  22. pmc Temporal and spatial analysis of clinical and molecular parameters in dextran sodium sulfate induced colitis
    Yutao Yan
    Division of Digestive Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA
    PLoS ONE 4:e6073. 2009
    ..In this study we systematically analyzed clinical parameters, histological changes, intestinal barrier properties and cytokine profile during the colitic and recovery phase...
  23. ncbi The pathophysiology of mitochondrial cell death
    Douglas R Green
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Science 305:626-9. 2004
    ..mitochondrial lipids, proteins that regulate bioenergetic metabolite flux, and putative components of the permeability transition pore...
  24. pmc Challenges and key considerations of the enhanced permeability and retention effect for nanomedicine drug delivery in oncology
    Uma Prabhakar
    Alliance for Nanotechnology in Cancer, National Cancer Institute, Bethesda, MD 20892, USA
    Cancer Res 73:2412-7. 2013
    Enhanced permeability of the tumor vasculature allows macromolecules to enter the tumor interstitial space, whereas the suppressed lymphatic filtration allows them to stay there...
  25. ncbi From structure to disease: the evolving tale of aquaporin biology
    Landon S King
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, The Johns Hopkins University School of Medicine, 5501 Hopkins Bayview Circle, Baltimore, Maryland 21224, USA
    Nat Rev Mol Cell Biol 5:687-98. 2004
    ..These proteins are present in organisms at all levels of life, and their unique permeability characteristics and distribution in numerous tissues indicate diverse roles in the regulation of water ..
  26. pmc Alterations in intestinal permeability
    M C Arrieta
    Department of Medicine, University of Alberta, Alberta, Canada
    Gut 55:1512-20. 2006
  27. pmc Regulated expression of claudin-4 decreases paracellular conductance through a selective decrease in sodium permeability
    C Van Itallie
    Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520 8019, USA
    J Clin Invest 107:1319-27. 2001
    ..potentials revealed that the decrease in paracellular conductance resulted from a selective decrease in Na(+) permeability without a significant effect on Cl(-) permeability...
  28. doi Vasoactive intestinal peptide ameliorates intestinal barrier disruption associated with Citrobacter rodentium-induced colitis
    V S Conlin
    Division of Gastroenterology, Child and Family Research Institute, BC Children s Hospital, Vancouver, BC, Canada
    Am J Physiol Gastrointest Liver Physiol 297:G735-50. 2009
    ..pathogens attach to the apical surface of epithelial cells and disrupt epithelial barrier function, increasing permeability and allowing luminal contents access to the underlying milieu...
  29. pmc SCaMC-1 promotes cancer cell survival by desensitizing mitochondrial permeability transition via ATP/ADP-mediated matrix Ca(2+) buffering
    J Traba
    Departamento de Biologia Molecular, Centro de Biología Molecular Severo Ochoa UAM CSIC, CIBER de Enfermedades Raras, Universidad Autonoma, Madrid, Spain
    Cell Death Differ 19:650-60. 2012
    Ca(2+)-mediated mitochondrial permeability transition (mPT) is the final common pathway of stress-induced cell death in many major pathologies, but its regulation in intact cells is poorly understood...
  30. pmc Myosin light chain kinase mediates intestinal barrier disruption following burn injury
    Chuanli Chen
    State Key Laboratory of Trauma, Burns, and Combined Injury, Institute of Burn Research, Third Military Medical University, Southwest Hospital, Chongqing, China
    PLoS ONE 7:e34946. 2012
    ..We hypothesized that the MLCK-dependent MLC phosphorylation mediates the regulation of intestinal barrier function following burn injury, and that MLCK inhibition attenuates the burn-induced intestinal barrier disfunction...
  31. pmc Dual role of COUP-TF-interacting protein 2 in epidermal homeostasis and permeability barrier formation
    Olga Golonzhka
    Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331, USA
    J Invest Dermatol 129:1459-70. 2009
    ..in keratinocyte proliferation and late differentiation events, as well as in the development of the epidermal permeability barrier...
  32. doi Specific role of tight junction proteins claudin-5, occludin, and ZO-1 of the blood-brain barrier in a focal cerebral ischemic insult
    Haixia Jiao
    Department of Neurobiology, College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning Province, People s Republic of China
    J Mol Neurosci 44:130-9. 2011
    ..ischemia model by transient middle cerebral artery occlusion in male rats for defining the time course of BBB permeability within 120 h following reperfusion and evaluate the specific role of tight junction (TJ) associated proteins ..
  33. doi Zonulin and its regulation of intestinal barrier function: the biological door to inflammation, autoimmunity, and cancer
    Alessio Fasano
    Mucosal Biology Research Center and Center for Celiac Research, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Physiol Rev 91:151-75. 2011
    ..This review is timely given the increased interest in the role of a "leaky gut" in the pathogenesis of several pathological conditions targeting both the intestine and extraintestinal organs...
  34. ncbi Corticotropin-releasing hormone (CRH) regulates macromolecular permeability via mast cells in normal human colonic biopsies in vitro
    C Wallon
    Department of Clinical and Experimental Medicine, Linkoping University, Linkoping, Sweden
    Gut 57:50-8. 2008
    ..The aim was to examine the effects of CRH on mucosal barrier function in the human colon and to elucidate the mechanisms involved in CRH-induced hyper-permeability.
  35. doi The food contaminant deoxynivalenol, decreases intestinal barrier permeability and reduces claudin expression
    Philippe Pinton
    INRA UR66, Toulouse, France
    Toxicol Appl Pharmacol 237:41-8. 2009
    ..electrical resistance (TEER) and increases in a time and dose-dependent manner the paracellular permeability to 4 kDa dextran and to pathogenic Escherichia coli across intestinal cell monolayers...
  36. ncbi IL-1beta regulates blood-brain barrier permeability via reactivation of the hypoxia-angiogenesis program
    Azeb Tadesse Argaw
    Corinne Goldsmith Dickinson Center for Multiple Sclerosis, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Immunol 177:5574-84. 2006
    ..endothelium forms the basis of the BBB, while astrocytes control the balance between barrier stability and permeability via production of factors that restrict or promote vessel plasticity...
  37. pmc The role of small intestinal bacterial overgrowth, intestinal permeability, endotoxaemia, and tumour necrosis factor alpha in the pathogenesis of non-alcoholic steatohepatitis
    A J Wigg
    Department of Gastroenterology, Queen Elizabeth Hospital, Adelaide, South Australia, Australia
    Gut 48:206-11. 2001
    Small intestinal bacterial overgrowth may contribute to the development of non-alcoholic steatohepatitis, perhaps by increasing intestinal permeability and promoting the absorption of endotoxin or other enteric bacterial products.
  38. ncbi Gastrointestinal dysfunction induced by early weaning is attenuated by delayed weaning and mast cell blockade in pigs
    Adam J Moeser
    Center for Comparative Translational and Molecular Research, College of Veterinary Medicine, North Carolina State Univ, 4700 Hillsborough St, Raleigh, NC 27606, USA
    Am J Physiol Gastrointest Liver Physiol 293:G413-21. 2007
    ..Early weaning resulted in reductions in transepithelial electrical resistance and increases in mucosal permeability to [(3)H]mannitol in the jejunum and colon (P < 0.01)...
  39. ncbi Toll-like receptor 2 controls mucosal inflammation by regulating epithelial barrier function
    E Cario
    Division of Gastroenterology and Hepatology, University Hospital of Essen and Medical School University of Duisburg Essen, Essen, Germany
    Gastroenterology 132:1359-74. 2007
    ..The aim of this study was to determine the functional relevance of TLR2 to control tight junction (TJ)-associated intestinal epithelial barrier integrity to balance mucosal homeostasis against inflammatory stress-induced damage...
  40. doi Intestinal barrier dysfunction in inflammatory bowel diseases
    Michael A McGuckin
    Mucosal Diseases Program, Mater Medical Research Institute, University of Queensland, Aubigny Place, Mater Health Services, South Brisbane, Queensland, Australia
    Inflamm Bowel Dis 15:100-13. 2009
    ....
  41. doi Accumulation of sub-100 nm polymeric micelles in poorly permeable tumours depends on size
    H Cabral
    Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Hongo, Bunkyo ku, Japan
    Nat Nanotechnol 6:815-23. 2011
    ..We also showed that the penetration and efficacy of the larger micelles could be enhanced by using a transforming growth factor-β inhibitor to increase the permeability of the tumours.
  42. ncbi Bid, Bax, and lipids cooperate to form supramolecular openings in the outer mitochondrial membrane
    Tomomi Kuwana
    La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Cell 111:331-42. 2002
    ..We conclude that mitochondrial protein release in apoptosis can be mediated by supramolecular openings in the outer mitochondrial membrane, promoted by BH3/Bax/lipid interaction and directly inhibited by Bcl-x(L)...
  43. ncbi Role of the mitochondrial membrane permeability transition in cell death
    Yoshihide Tsujimoto
    Osaka University Medical School, Department of Medical Genetics, SORST of the Japan Science and Technology Agency, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Apoptosis 12:835-40. 2007
    ..An increase of mitochondrial membrane permeability is one of the key events in apoptotic or necrotic death, although the details of the mechanism involved remain ..
  44. doi Increased intestinal permeability and tight junction alterations in nonalcoholic fatty liver disease
    Luca Miele
    Institute of Internal Medicine, Catholic University of Rome, Rome, Italy
    Hepatology 49:1877-87. 2009
    ..We investigated intestinal permeability in patients with NAFLD and evaluated the correlations between this phenomenon and the stage of the disease, the ..
  45. pmc Differentially altered Ca2+ regulation and Ca2+ permeability in Cx26 hemichannels formed by the A40V and G45E mutations that cause keratitis ichthyosis deafness syndrome
    Helmuth A Sánchez
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Gen Physiol 136:47-62. 2010
    ..hemichannels show only modest impairment in regulation by Ca(2+) and instead exhibit a substantial increase in permeability to Ca(2+)...
  46. ncbi The mitochondrial permeability transition initiates autophagy in rat hepatocytes
    S P Elmore
    Department of Cell and Developmental Biology and Curriculum in Toxicology, University of North Carolina, Chapel Hill, NC 27599, USA
    FASEB J 15:2286-7. 2001
    ..Cyclosporin A (CsA, 5 M), an immunosuppressant that blocks the mitochondrial permeability transition (MPT), prevented this depolarization...
  47. pmc Permeability dependence study of the focused ultrasound-induced blood-brain barrier opening at distinct pressures and microbubble diameters using DCE-MRI
    Fotios Vlachos
    Department of Biomedical Engineering, Columbia University, New York 10027, USA
    Magn Reson Med 66:821-30. 2011
    ..In this study, the permeability of the opening is assessed in the murine hippocampus after the application of focused ultrasound at three ..
  48. doi Mitochondrial permeability transition in Ca(2+)-dependent apoptosis and necrosis
    Andrea Rasola
    Department of Biomedical Sciences and CNR Institute of Neuroscience, University of Padova, Italy
    Cell Calcium 50:222-33. 2011
    ..sensitizing factors of pathophysiological relevance, the matrix Ca(2+) increase can also lead to opening of the permeability transition pore (PTP), a high conductance inner membrane channel...
  49. doi Endothelial glycocalyx: permeability barrier and mechanosensor
    F E Curry
    Department of Physiology and Membrane Biology, School of Medicine, University of California at Davis, 1 Shields Avenue, Davis, CA 95616, USA
    Ann Biomed Eng 40:828-39. 2012
    ..This review compares the mechanosensor and permeability properties of an inner layer (100-150 nm, close to the endothelial membrane) characterized as a quasi-periodic ..
  50. ncbi The mitochondrial permeability transition in cell death: a common mechanism in necrosis, apoptosis and autophagy
    J J Lemasters
    Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill, CB No 7090, 236 Taylor Hall, Chapel Hill, NC 27799 7090, USA
    Biochim Biophys Acta 1366:177-96. 1998
    Using confocal microscopy, onset of the mitochondrial permeability transition (MPT) in individual mitochondria within living cells can be visualized by the redistribution of the cytosolic fluorophore, calcein, into mitochondria...
  51. doi Review article: Associations between immune activation, intestinal permeability and the irritable bowel syndrome
    J Matricon
    Clermont Universite, Universite d Auvergne, NEURO DOL, Clermont Ferrand, France INSERM, Clermont Ferrand, France
    Aliment Pharmacol Ther 36:1009-31. 2012
    ..Drug development for IBS treatment has been hampered by the lack of understanding of IBS aetiology. In recent years, numerous data have emerged that suggest the involvement of immune activation in IBS, at least in a subset of patients...
  52. pmc High-resolution longitudinal assessment of flow and permeability in mouse glioma vasculature: Sequential small molecule and SPIO dynamic contrast agent MRI
    M M Pike
    Department of Medicine, Division of Cardiovascular Disease, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Magn Reson Med 61:615-25. 2009
    ..in the intracranial 4C8 mouse glioma model to determine K(trans) and v(e), indices of tumor vascular permeability and cellularity, respectively...
  53. ncbi Increased permeability of the blood-brain barrier to chemotherapy in metastatic brain tumors: establishing a treatment paradigm
    Elizabeth R Gerstner
    Department of Neurology, Neurological Institute of New York, New York, NY, USA
    J Clin Oncol 25:2306-12. 2007
    ..This information could lead to a paradigm shift in the use of natural product drugs for metastatic brain tumors...
  54. pmc Membrane-anchored serine protease matriptase regulates epithelial barrier formation and permeability in the intestine
    Marguerite S Buzza
    Department of Physiology, Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Proc Natl Acad Sci U S A 107:4200-5. 2010
    ..by siRNA disrupts the development of TEER in barrier-forming Caco-2 monolayers and increases paracellular permeability to macromolecular FITC-dextran...
  55. doi Caveolin-1 regulates nitric oxide-mediated matrix metalloproteinases activity and blood-brain barrier permeability in focal cerebral ischemia and reperfusion injury
    Yong Gu
    School of Chinese Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
    J Neurochem 120:147-56. 2012
    The roles of caveolin-1 (cav-1) in regulating blood-brain barrier (BBB) permeability are unclear yet...
  56. pmc MicroRNA-29a regulates intestinal membrane permeability in patients with irritable bowel syndrome
    QiQi Zhou
    Department of Medicine, Ohio State University, 1799 W 5th Avenue, Columbus, OH 43212, USA
    Gut 59:775-84. 2010
    ..One mechanism may involve increased intestinal permeability that is reversed with glutamine supplementation...
  57. ncbi Cytosolic pH regulates root water transport during anoxic stress through gating of aquaporins
    Colette Tournaire-Roux
    Biochimie et Physiologie Moleculaire des Plantes, Centre National de la Recherche Scientifique Unité Mixte de Recherche 5004, Institut National de la Recherche Agronomique, Université Montpellier 2 et Ecole Nationale d Agronomie, Montpellier, France
    Nature 425:393-7. 2003
    ..to anoxia and other environmental stresses is downregulation of water uptake due to inhibition of the water permeability (hydraulic conductivity) of roots (Lp(r))...
  58. pmc Early weaning stress impairs development of mucosal barrier function in the porcine intestine
    Feli Smith
    Department of Population Health and Pathobiology, North Carolina State University, Raleigh, 27606, USA
    Am J Physiol Gastrointest Liver Physiol 298:G352-63. 2010
    ....
  59. doi Regulation of intestinal epithelial permeability by tight junctions
    Takuya Suzuki
    Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, 1 4 4, Kagamiyama, Higashi Hiroshima, 739 8528, Japan
    Cell Mol Life Sci 70:631-59. 2013
    ..permeable barrier is achieved by intercellular tight junction (TJ) structures, which regulate paracellular permeability. Disruption of the intestinal TJ barrier, followed by permeation of luminal noxious molecules, induces a ..
  60. pmc Ecto-5'-nucleotidase (CD73) regulation by hypoxia-inducible factor-1 mediates permeability changes in intestinal epithelia
    Kristin Synnestvedt
    Center for Experimental Therapeutics and Reperfusion Injury, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Clin Invest 110:993-1002. 2002
    ..protect the epithelial barrier, since the CD73 inhibitor alpha,beta-methylene ADP promotes increased intestinal permeability. These results identify an HIF-1-dependent regulatory pathway for CD73 and indicate the likelihood that CD39/..
  61. pmc Gliadin induces an increase in intestinal permeability and zonulin release by binding to the chemokine receptor CXCR3
    Karen M Lammers
    Mucosal Biology Research Center, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Gastroenterology 135:194-204.e3. 2008
    ..Gliadin induces an MyD88-dependent zonulin release that leads to increased intestinal permeability, a postulated early element in the pathogenesis of celiac disease...
  62. pmc IL-1beta causes an increase in intestinal epithelial tight junction permeability
    Rana M Al-Sadi
    Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    J Immunol 178:4641-9. 2007
    ..studies have indicated that a TNF-alpha- and IFN-gamma-induced increase in intestinal epithelial paracellular permeability may be an important mechanism contributing to intestinal inflammation...
  63. pmc Role of critical thiol groups on the matrix surface of the adenine nucleotide translocase in the mechanism of the mitochondrial permeability transition pore
    Gavin P McStay
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, U K
    Biochem J 367:541-8. 2002
    Opening of the mitochondrial permeability transition pore (MPTP) is sensitized to [Ca(2+)] by oxidative stress (diamide) and phenylarsine oxide (PAO)...
  64. ncbi Gliadin, zonulin and gut permeability: Effects on celiac and non-celiac intestinal mucosa and intestinal cell lines
    Sandro Drago
    Mucosal Biology Research Center, Center for Celiac Research and Division of Pediatric Gastroenterology and Nutrition, University of Maryland, School of Medicine, Baltimore, MD 21201, USA
    Scand J Gastroenterol 41:408-19. 2006
    ..We investigated whether gliadin has any immediate effect on zonulin release and signaling...
  65. ncbi Role of the mitochondrial permeability transition in myocardial disease
    James N Weiss
    Cardiovascular Research Laboratory, Department of Medicine Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, Calif 90095 1760, USA
    Circ Res 93:292-301. 2003
    ..the mitochondrial pathway and necrosis by irreversible damage to mitochondria in association with mitochondrial permeability transition (MPT)...
  66. ncbi HIV-1 gp120 proteins alter tight junction protein expression and brain endothelial cell permeability: implications for the pathogenesis of HIV-associated dementia
    Georgette D Kanmogne
    Department of Pharmacology, Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, Nebraska 68198 5215, USA
    J Neuropathol Exp Neurol 64:498-505. 2005
    ..The objective of this study is to determine the effects of gp120 proteins on brain endothelial cell permeability and junctional protein expression...
  67. ncbi Direct effect of Taxol on free radical formation and mitochondrial permeability transition
    G Varbiro
    Institute of Biochemistry, University of Pecs, Medical School, Pecs, Hungary
    Free Radic Biol Med 31:548-58. 2001
    ..Opening of the permeability pore was also confirmed by the access of mitochondrial matrix enzymes for membrane impermeable substrates in ..
  68. doi Regulation of endothelial permeability via paracellular and transcellular transport pathways
    Yulia Komarova
    Department of Pharmacology and Center for Lung and Vascular Biology, University of Illinois College of Medicine, Chicago, IL 60612, USA
    Annu Rev Physiol 72:463-93. 2010
    ..The permeability of the endothelial barrier is an exquisitely regulated process in the resting state and in response to ..
  69. ncbi Mitochondrial control of cell death
    G Kroemer
    Centre National de la Recherche Scientifique, CNRS ULR1599, Institut Gustave Roussy 24 rue Calmette Desmoulins, F 94805 Villejuif
    Nat Med 6:513-9. 2000
    ..In many instances, permeabilization of mitochondrial membranes is a rate-limiting step of apoptotic or necrotic cell demise. This has important consequences for the pathophysiology of cell death, as well as for its pharmacological control...
  70. pmc Methamphetamine alters blood brain barrier permeability via the modulation of tight junction expression: Implication for HIV-1 neuropathogenesis in the context of drug abuse
    Supriya D Mahajan
    Department of Medicine, Division of Allergy, Immunology, and Rheumatology, 301 Multi Research Building, Buffalo General Hospital, 100 High Street, Buffalo, NY 14203, USA
    Brain Res 1203:133-48. 2008
    ..We hypothesize that gp120 in synergy with Meth can alter BBB permeability via the modulation of tight junction expression...
  71. doi Enhanced permeability and retention (EPR) effect for anticancer nanomedicine drug targeting
    Khaled Greish
    Department of Pharmaceuticals and Pharmaceutical Chemistry, and Utah Center for Nanomedicine, University of Utah, Salt Lake City, UT, USA
    Methods Mol Biol 624:25-37. 2010
    ..vasculature, namely hypervascularization, aberrant vascular architecture, extensive production of vascular permeability factors stimulating extravasation within tumor tissues, and lack of lymphatic drainage...
  72. doi Intestinal permeability in coeliac disease: insight into mechanisms and relevance to pathogenesis
    Martine Heyman
    INSERM U989, Universite Paris Descartes, Paris, France
    Gut 61:1355-64. 2012
    ..These recent findings might be useful for the design of new treatments...
  73. pmc Mitofusins and the mitochondrial permeability transition: the potential downside of mitochondrial fusion
    Kyriakos N Papanicolaou
    Whitaker Cardiovascular Institute, Boston University School of Medicine, Massachusetts, 02118, USA
    Am J Physiol Heart Circ Physiol 303:H243-55. 2012
    ..mitochondrial membrane destabilization that is opportunistically employed during the process of mitochondrial permeability transition...
  74. ncbi Dialyzer membrane permeability and survival in hemodialysis patients
    Philippe Chauveau
    Departement de Nephrologie, Centre Hospitalier Universitaire and Université Bordeaux 2, Bordeaux, France
    Am J Kidney Dis 45:565-71. 2005
    ..Information on dialysis membrane and erythropoietin use had to be evaluated in an additional follow-up...
  75. pmc Membrane fusion catalyzed by a Rab, SNAREs, and SNARE chaperones is accompanied by enhanced permeability to small molecules and by lysis
    Paola C Zucchi
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755 3844, USA
    Mol Biol Cell 22:4635-46. 2011
    ..dithionite), we find that yeast vacuolar SNAREs (SNAP [Soluble NSF attachment protein] Receptors) increase the permeability of membranes to small molecules and that this permeabilization is enhanced by homotypic fusion and vacuole ..
  76. pmc Hepatitis C virus triggers mitochondrial permeability transition with production of reactive oxygen species, leading to DNA damage and STAT3 activation
    Keigo Machida
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, 90033, USA
    J Virol 80:7199-207. 2006
    ..These HCV studies thus identified ROS, along with the previously identified NO, as the primary inducers of DSBs and mitochondrial damage in HCV-infected cells...
  77. doi Ceramide and activated Bax act synergistically to permeabilize the mitochondrial outer membrane
    Vidyaramanan Ganesan
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
    Apoptosis 15:553-62. 2010
    ..These and previous findings that anti-apoptotic proteins disassemble ceramide channels suggest that ceramide channels, regulated by Bcl-2-family proteins, may be responsible for the MOMP during apoptosis...
  78. pmc Treatment with docosahexaenoic acid, but not eicosapentaenoic acid, delays Ca2+-induced mitochondria permeability transition in normal and hypertrophied myocardium
    Ramzi J Khairallah
    Division of Cardiology and Department of Medicine, University of Maryland, Baltimore, MD 21201, USA
    J Pharmacol Exp Ther 335:155-62. 2010
    ..Mitochondrial permeability transition pore (MPTP) opening contributes to myocardial pathology in cardiac hypertrophy and heart failure, ..
  79. pmc No facilitator required for membrane transport of hydrogen sulfide
    John C Mathai
    Beth Israel Deaconess Medical Center and Harvard Medical School, 330 Brookline Avenue, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 106:16633-8. 2009
    ..A lower limit of lipid bilayer permeability to H(2)S, P(M,H(2)S) >or = 0.5 +/- 0...
  80. doi TRO40303, a new cardioprotective compound, inhibits mitochondrial permeability transition
    Sophie Schaller
    Trophos S A, Luminy Biotech Entreprises, Marseille, France
    J Pharmacol Exp Ther 333:696-706. 2010
    ..the calcium retention capacity of isolated mitochondria, unlike cyclosporine A, the drug delayed mitochondrial permeability transition pore (mPTP) opening and cell death in isolated adult rat cardiomyocytes subjected to 2 h of hypoxia ..
  81. pmc Erythropoietin modulation of astrocyte water permeability as a component of neuroprotection
    Eli Gunnarson
    Department of Woman and Child Health, Karolinska Institutet, 17176 Stockholm, Sweden
    Proc Natl Acad Sci U S A 106:1602-7. 2009
    ..have shown that glutamate, acting on group I metabotropic glutamate receptors (mGluRs), increases the permeability of astrocyte AQP4, which, in situations of hypoxia-ischemia, will increase astrocyte water uptake...
  82. pmc Lactobacillus GG treatment ameliorates alcohol-induced intestinal oxidative stress, gut leakiness, and liver injury in a rat model of alcoholic steatohepatitis
    Christopher B Forsyth
    Department of Internal Medicine, Division of Digestive Diseases and Nutrition, and Rush University Medical Center, 1725 W Harrison, Suite 206, Chicago, IL 60612, USA
    Alcohol 43:163-72. 2009
    ..5 x 10(7) live L. rhamnosus Gorbach-Goldin (LGG) or vehicle (V). Intestinal permeability (baseline and at 10 weeks) was determined using a sugar bolus and GC analysis of urinary sugars...
  83. pmc Permeability of endothelial and astrocyte cocultures: in vitro blood-brain barrier models for drug delivery studies
    Guanglei Li
    Department of Biomedical Engineering, The City College of the City University of New York, New York, NY 10031, USA
    Ann Biomed Eng 38:2499-511. 2010
    ..the hydraulic conductivity (L (p)), transendothelial electrical resistance (TER) and diffusive solute permeability (P) of these models to three solutes: TAMRA, Dextran 10K and Dextran 70K...
  84. pmc Bcl-2 proteins regulate ER membrane permeability to luminal proteins during ER stress-induced apoptosis
    X Wang
    Molecular Targets Group, James Graham Brown Cancer Center, Department of Medicine, University of Louisville, Louisville, KY 40202, USA
    Cell Death Differ 18:38-47. 2011
    ..In addition, Bax and truncated-Bid (tBid) mediate a global increase in ER membrane permeability to ER luminal proteins in vitro...
  85. ncbi Mast cell tryptase controls paracellular permeability of the intestine. Role of protease-activated receptor 2 and beta-arrestins
    Claire Jacob
    Department of Surgery, University of California, San Francisco, California 94143, USA
    J Biol Chem 280:31936-48. 2005
    ..During stress and inflammation, mast cells mediate increased mucosal permeability by unknown mechanisms...
  86. doi Analysis of cell viability in intervertebral disc: Effect of endplate permeability on cell population
    A Shirazi-Adl
    Department of Mechanical Engineering, Ecole Polytechnique, P O Box 6079, Station Centre Ville, Montreal, Quebec, Canada H3C 3A7
    J Biomech 43:1330-6. 2010
    ..In cases with loss of endplate permeability and/or disruptions therein, as well as changes in geometry and fall in diffusivity associated with fluid ..
  87. doi Multiple facets of intestinal permeability and epithelial handling of dietary antigens
    S Menard
    INSERM, U989, Interactions of the intestinal epithelium with the immune system, Universite Paris Descartes, Paris, Cedex 15, France
    Mucosal Immunol 3:247-59. 2010
    ..and/or perpetuate chronic inflammation as well as the respective contribution of paracellular and transcellular permeability remains a matter of debate...
  88. doi Melatonin-loaded lecithin/chitosan nanoparticles: physicochemical characterisation and permeability through Caco-2 cell monolayers
    Anita Hafner
    Department of Pharmaceutics, Faculty of Pharmacy and Biochemistry, University of Zagreb, A Kovacica 1, 10000 Zagreb, Croatia
    Int J Pharm 381:205-13. 2009
    ..Approximately 60-70% of melatonin was released in 4h. The permeability of melatonin was investigated using Caco-2 cells as an in vitro model of the epithelial barrier...
  89. pmc Roles of mitophagy and the mitochondrial permeability transition in remodeling of cultured rat hepatocytes
    Sara Rodriguez-Enriquez
    Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC, USA
    Autophagy 5:1099-106. 2009
    ..an inhibitor of autophagy, and also by cyclosporin A and NIM811, both selective inhibitors of the mitochondrial permeability transition (MPT)...
  90. doi The mitochondrial permeability transition, and oxidative and nitrosative stress in the mechanism of copper toxicity in cultured neurons and astrocytes
    Pichili V B Reddy
    Department of Pathology, University of Miami School of Medicine, Miami, FL 33101, USA
    Lab Invest 88:816-30. 2008
    ..As oxidative stress is an important factor in the induction of the mitochondrial permeability transition (mPT), we determined whether mPT plays a role in copper-induced neural cell injury...
  91. ncbi Disruption of the outer mitochondrial membrane as a result of large amplitude swelling: the impact of irreversible permeability transition
    P X Petit
    CNRS UPR420, Génétique Moléculaire et Biologie du Développement 19, Villejuif, France
    FEBS Lett 426:111-6. 1998
    Upon induction of permeability transition with different agents (Ca2+, tert-butyl hydroperoxide, atractyloside), mouse hepatocyte mitochondria manifest a disruption of outer membrane integrity leading to the release of cytochrome c and ..
  92. ncbi Water permeation across biological membranes: mechanism and dynamics of aquaporin-1 and GlpF
    B L de Groot
    Theoretical Molecular Biophysics Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Science 294:2353-7. 2001
    ..In AQP1, a fine-tuned water dipole rotation during passage is essential for water selectivity. In GlpF, a glycerol-mediated "induced fit" gating motion is proposed to generate selectivity for glycerol over water...
  93. ncbi Genetic dissection of the permeability transition pore
    Michael Forte
    Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    J Bioenerg Biomembr 37:121-8. 2005
    The permeability transition pore (PTP) regulates the structural re-organization of mitochondria in response to changes in cellular Ca(2+) and is thought to be an important participant in mitochondrial responses to cell death signals...
  94. ncbi Astrocyte mediated modulation of blood-brain barrier permeability does not correlate with a loss of tight junction proteins from the cellular contacts
    Stefan Hamm
    Max Planck Institute for Physiological and Clinical Research, Bad Nauheim, Germany
    Cell Tissue Res 315:157-66. 2004
    ..borders, we used an in vitro BBB model where brain endothelial cells in co-culture with astrocytes form a tight permeability barrier for 3H-inulin and 14C-sucrose...
  95. ncbi Predicting blood-brain barrier permeability of drugs: evaluation of different in vitro assays
    C Lohmann
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Germany
    J Drug Target 10:263-76. 2002
    ..Five different in vitro methods to predict BBB permeability of drugs were compared and evaluated in the present study...
  96. ncbi New water-soluble phosphines as reductants of peptide and protein disulfide bonds: reactivity and membrane permeability
    Daniel J Cline
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 43:15195-203. 2004
    ..In sum, these data suggest that there is considerable scope for the synthesis of phosphine analogues tailored for specific applications in biological systems...
  97. ncbi Molecular properties that influence the oral bioavailability of drug candidates
    Daniel F Veber
    Department of Medicinal Chemistry, GlaxoSmithKline, 709 Swedeland Road, P O Box 1539, King of Prussia, Pa 19406 0939, USA
    J Med Chem 45:2615-23. 2002
    ..A threshold permeation rate is a prerequisite of oral bioavailability. The rotatable bond count does not correlate with the data examined here for the in vivo clearance rate in the rat...
  98. ncbi Allopregnanolone modulates spontaneous GABA release via presynaptic Cl- permeability in rat preoptic nerve terminals
    David Haage
    Department of Integrative Medical Biology, Section for Physiology, Umea University, S 901 87 Umea, Sweden
    Brain Res 958:405-13. 2002
    ..Altogether, the results suggest that allopregnanolone exerts its presynaptic effect by increasing the presynaptic Cl(-) permeability, most likely via GABA(A) receptors.
  99. ncbi Ion permeation mechanism of the potassium channel
    J Aqvist
    Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Sweden
    Nature 404:881-4. 2000
    ..Other putative permeation pathways can be excluded because they would involve states that are too high in energy...
  100. doi Influence of a high-fat diet on gut microbiota, intestinal permeability and metabolic endotoxaemia
    Ana Paula Boroni Moreira
    Nutrition and Health Department, Federal University of Vicosa, Minas Gerais, Avenida PH Rolfs s n, Vicosa, Minas Gerais, Brazil
    Br J Nutr 108:801-9. 2012
    ..by which LPS move into the circulatory system is either through direct diffusion due to intestinal paracellular permeability or through absorption by enterocytes during chylomicron secretion...
  101. ncbi From calcium signaling to cell death: two conformations for the mitochondrial permeability transition pore. Switching from low- to high-conductance state
    F Ichas
    INSERM CJF 9705, Integrated Biological Systems Study Group, Victor Segalen Bordeaux 2 University, 146 rue Leo Saignat, F 33076 Bordeaux cedex, France
    Biochim Biophys Acta 1366:33-50. 1998
    The permeability transition pore (PTP) is a channel of the inner mitochondrial membrane that appears to operate at the crossroads of two distinct physiological pathways, i.e...

Research Grants71

  1. Discovery metabolite profiling of the prolyl peptidases
    Alan Saghatelian; Fiscal Year: 2009
    ..Furthermore, the application of DMP to peptidases will demonstrate the generality of this approach for the future characterization of medically relevant enzymes and signaling pathways. ..
  2. SUPPORT CONTRACT FOR THE NTP INTERAGENCY CENTER/VALIDATION OF ALT METHODS
    David Allen; Fiscal Year: 2011
    ..In 2008, two ICCVAM-recommended in vitro safety testing methods, the bovine corneal opacity and permeability (BCOP) and isolated chicken eye (ICE) test methods, were accepted by U.S...
  3. SUPPORT CONTRACT FOR THE NTP INTERAGENCY CENTER/VALIDATION OF ALT METHODS
    David Allen; Fiscal Year: 2012
    ..In 2008, two ICCVAM-recommended in vitro safety testing methods, the bovine corneal opacity and permeability (BCOP) and isolated chicken eye (ICE) test methods, were accepted by U.S...
  4. Cardiotrophin-Like Cytokine 1, a Candidate Molecule for the FSGS Factor
    Virginia J Savin; Fiscal Year: 2010
    ..We have documented that serum or plasma from FSGS patients increases glomerular albumin permeability (Palb) and that the specific activity of certain protein fractions of FSGS plasma is more than 10,000-fold ..
  5. Multi-targeted Countermeasures against Acute and Delayed Effects of OP Exposure
    John A Butera; Fiscal Year: 2013
    ..for simultaneous multi-therapeutic modality treatments with complicated dosing regiments, (2) poor CNS-permeability of currently used pyridinium oximes, and (3) the complete lack of efficacy in any of the current treatments to ..
  6. Brain mast cells and Chronic Fatigue Syndrome
    Theoharis C Theoharides; Fiscal Year: 2013
    ..mast cells through CRHR-1 leading to release of vascular endothelial growth factor (VEGF), increased vascular permeability and blood-brain-barrier (BBB) disruption...
  7. A Hybrid PARP Inhibitor and Redox Catalyst for Lung Transplantation
    GARRY JOHN SOUTHAN; Fiscal Year: 2012
    ..PARP activation also induces a widespread expresion of pro-inflammatory genes that contribute to vascular permeability, lung edema, neutrophil infiltration, pulmonary shunt, and respiratory failure...
  8. Roles of Nitric Oxide and Superoxide in Cystitis
    ANTHONY JOHN KANAI; Fiscal Year: 2013
    ..cystitis includes inflammation and bladder overactivity resulting from disruption of the urothelial cell (UC) permeability barrier and sensitization of afferent nerves...
  9. A Redox-active PARP Inhibitor for Lung Transplantation
    ANDREW LURIE SALZMAN; Fiscal Year: 2012
    ..PARP-1 activation also induces a widespread expression of pro-inflammatory genes that contribute to vascular permeability, lung edema, neutrophil infiltration, pulmonary shunt, and respiratory failure...
  10. TLR4 Inhibition to Reduce Cerebral Ischemia Reperfusion Injury
    LORI ANN HOLLE; Fiscal Year: 2011
    ..IRI is associated with early increased endothelial permeability and edema and later, changes in gene expression, resulting in inflammation...
  11. Dengue Infected Endothelial Cells Enhance Immune Cell Activation
    Erich R Mackow; Fiscal Year: 2013
    ..Thus an immune enhanced disease process contributes to increased endothelial cell (EC) permeability in DHF/DSS cases...
  12. PATHOPHYSIOLOGY OF THE ENDOTHELIUM
    Francis W Luscinskas; Fiscal Year: 2013
    ..particularly important in the context of inflammation, are the regulation of leukocyte recruitment and vascular permeability, both of which involve coordinated interactions of cell-cell adhesion/junctional molecules and the actin ..
  13. Small Molecule targeting of NADPH oxidase in neutrophils
    Marie Dominique Filippi; Fiscal Year: 2010
    ..manifestation of ARDS and ALI is edema, which results from endothelial dysfunction and microvasculature permeability. This is caused by release of free radical and reactive oxygen species (ROS) and inflammatory cytokines (e.g...
  14. Mechanism-Based Discovery of Protectants Against Iron-Dependant Oxidative Injury
    JUSTIN M SCHWARTZ; Fiscal Year: 2011
    ..this iron via the electrogenic calcium uniporter to promote intramitochondrial OH7 formation, the mitochondrial permeability transition (MPT) and ultimately cell death...
  15. Conditional HSF1 Expression for Ischemic Cardioprotection
    Ivor J Benjamin; Fiscal Year: 2012
    ..shock factor 1 (caHSF1) expression represents a novel chaperone-dependent pathway for preserving mitochondrial permeability transition and cellular protection...
  16. Role of the kallikrein-kinin system in diabetic retinopathy
    Edward P Feener; Fiscal Year: 2012
    Excessive retinal vascular permeability contributes to the pathogenesis of diabetic macular edema (DME), a leading cause of vision loss in working-age adults...
  17. Alcohol, Proteasome Dysfunction in Liver Injury
    Craig J McClain; Fiscal Year: 2013
    ..Additionally, this apoptotic death involves the induction of lysosomal membrane permeability (LMP) and is mediated by lysosomal proteases (cathepsins B and D)...
  18. LIVER PRESERVATION FOR TRANSPLANTATION
    John J Lemasters; Fiscal Year: 2012
    ..Our preliminary experiments indicate that the onset of the mitochondrial permeability transition (MPT) plays a key role in delayed parenchymal cell killing...
  19. Effect of Lactobacillus GG on gut permeability and microbiome in VLBW neonates
    ROSE MARIE VISCARDI; Fiscal Year: 2013
    ..the proposed study will assess the utility of an easily collected novel biomarker zonulin to monitor intestinal permeability, and provide information on the safety and acceptability of administering 3 different doses of LGG to VLBW ..
  20. Dual-Receptor Targeted Nanoparticles for Photodynamic Therapy of Brain Cancer
    Ann Marie Broome; Fiscal Year: 2013
    ..cell death using a mitochondrial membrane potential assay, TUNEL and DNA fragmentation assays, and cell permeability assays, including trypan blue staining and Annexin V/ propidium iodide flow cytometry...
  21. Abnormal Bladder Epithelial Cell Gene Expression in BPS/IC
    SUSAN F KEAY; Fiscal Year: 2013
    ..Because APF can induce the same changes in proliferation and permeability, as well as many of the consistent gene expression abnormal- ities found in BPS/IC bladder cells in vivo and/or ..
  22. Intranasal CNS delivery of drugs against organophosphorous threat agents
    ARYAN MANGALAM NAMBOODIRI; Fiscal Year: 2013
    ..The two major limitations of the current therapeutic strategies are: 1) poor blood-brain barrier (BBB) permeability of oximes and 2) resistance for reactivation of the CTA-inhibited AChE due to rapid 'aging'...
  23. Brain Delivery of Nanoformulated Superoxide Dismutase
    Erin G Rosenbaugh; Fiscal Year: 2013
    ..CuZnSOD with polyethylene glycol (PEG) to improve its circulating half-life;however, PEGylation decreases the permeability of proteins across the blood-brain barrier (BBB)...
  24. CT Evaluation of the Myocardial Effects of Risk Factors
    Lilach O Lerman; Fiscal Year: 2013
    ..Micro-CT will then be used to reconstruct myocardial microvessels in situ, and assess their permeability and myocardial lipid accumulation...
  25. Tumor Cell Arrest and Adhesion in the Microcirculation
    Bingmei M Fu; Fiscal Year: 2013
    ..research is to elucidate the relationships between microcirculation-induced mechanical factors, microvascular permeability (vascular integrity), cell adhesion molecules, nitric oxide and cytokines, and tumor metastasis in intact ..
  26. Role of Vimentin in Pathogenesis of CNS Inflammation
    Sheng He Huang; Fiscal Year: 2013
    ..Most importantly, we have shown that IbeA+ E. coli-induced NF-kB activation, bacterial invasion, PMNT and BBB permeability are significantly reduced in Vim KO (-/-) mice, which are consistent with the in vitro findings...
  27. ANGIOTENSIN II SIGNAL TRANSDUCTION
    Bradford C Berk; Fiscal Year: 2012
    ..In addition to the role for GIT1 in VSMC, we found that GIT1 mediated EC permeability and signaling by VEGF further defining the importance of GIT1 in vascular biology...
  28. Novel Diagnostics to Detect Lung Injury
    Elizabeth R Jacobs; Fiscal Year: 2013
    ..and some indices of mitochondrial dysfunction (iii) treatment with agents that prevent opening of mitochondrial permeability transition pore (mPTP) and those that are used clinically to prevent rejection will protect against lung injury ..
  29. Irritable bowel syndrome-diarrhea: the role of gluten intolerance and HLA-DQ2
    MICHAEL L CAMILLERI; Fiscal Year: 2010
    ..The link between gluten or gliadin and inflammation may be the increase in intestinal permeability, which is well established in celiac disease and involves binding to the chemokine receptor, CXCR3, leading to ..
  30. Nanoelectrode arrays for study of the molecular mechanisms, triggers, and inhibit
    Peter J Burke; Fiscal Year: 2012
    ..Mitochondria are the central regulator of apoptosis, a process initiated by the activation of the mitochondrial permeability transition pore (mtPTP), an aggregate of several mitochondrial proteins...
  31. Sphingosine 1-phosphate receptors in vascular homeostasis and pathology
    TIMOTHY TUN HLA; Fiscal Year: 2012
    ..cell-cell adhesion molecule function, promotes vascular maturation/stabilization and inhibits vascular permeability. In contrast, we found that S1P2R, which activates the Rho GTPase- dependent activation of the tumor suppressor ..
  32. Mitochondrial Mechanisms of Programmed Necrosis in the Aged Female Heart
    Alexandra M Machikas; Fiscal Year: 2013
    ..also includes a key role for programmed necrosis (PN) in the regulation of cell death via the mitochondrial permeability transition pore's (PTP) sensitivity to calcium overload, excess reactive oxygen species (ROS), and ATP ..
  33. Role of TLR-4 in Lung Reperfusion Injury
    MICHAEL SCOTT MULLIGAN; Fiscal Year: 2013
    ..Lung injury will be characterized by vascular permeability, inflammatory cell infiltration, histology, SAPK activation, NF[unreadable]B translocation and inflammatory ..
  34. Zot, Zonulin, and Pathophysiology of Intestinal Tight Junctions
    Alessio Fasano; Fiscal Year: 2013
    ..Genome wide association studies have coupled genes controlling gut permeability to autoimmune and inflammatory diseases...
  35. Delivery issues in lung cancer CNS metastases
    Edward A Neuwelt; Fiscal Year: 2013
    ..of the vasculature in individual metastases and peritumoral brain, in terms of both blood volume and vessel permeability, determines and limits the efficacy of chemo-radiotherapy treatments...
  36. Regulation/Role of AcylCer in Normal Epidermis and Atopic Dermatitis
    Peter M Elias; Fiscal Year: 2013
    ..e., stratum corneum (SC), subserve epidermal permeability barrier function, as required for mammalian survival in a dry environment...
  37. MRI of Acute Vascular Injury and Hemorrhagic Transformation in Ischemic Stroke
    Robert A Knight; Fiscal Year: 2010
    ..at approximately 2, 5 and 48 hr after ischemia onset;2) the influx of Gd-DTPA across the BBB (a measure of permeability) at approximately 5 and 48 hr after ischemia onset;and 3) ischemic injury at 48 hr as determined by ..
  38. MECHANISMS OF RETINAL VASCULAR PERMEABILITY IN DIABETES
    DAVID ANTONETTI; Fiscal Year: 2013
    ..The goal of this proposal is to understand the molecular mechanisms that lead to increased vascular permeability and macular edema and to elucidate the relationship of vascular permeability to angiogenesis so that novel ..
  39. Development of a novel method for inhibiting diabetic retinopathy
    David R Clemmons; Fiscal Year: 2012
    ..endothelial cells utilize both the PI-3 and MAP kinase pathways to activate changes in endothelial cell permeability and angiogenesis...
  40. MECHANISM OF ENDOTOXIN ABSORPTION IN ALCOHOLISM
    Radhakrishna Rao; Fiscal Year: 2013
    ..provided by applicant): Evidence from clinical and experimental studies indicates that elevated intestinal permeability to endotoxins and the resulting endotoxemia play a crucial role in the pathogenesis of alcoholic liver disease...
  41. Modulation of Colonic Epithelial Permeability by Sex Hormones
    Muriel Larauche; Fiscal Year: 2013
    ..alterations of the immune (mast cells) and epithelial (ion channels and secretion, tight junctions and permeability) systems related to sex hormones. The hypothesis will be tested in the following specific aims...
  42. Adhesion of Leptospira interrogans to Human Cell Surface Receptors
    Jenifer L Coburn; Fiscal Year: 2010
    ..These changes lead to increased permeability of the endothelial layers to the pathogen, without evidence of necrosis or apoptosis...
  43. Multimodal Retinal Functional Imaging for Diabetic Retinopathy
    Hao F Zhang; Fiscal Year: 2013
    ..This capillary damage leads to an increase in retinal vascular permeability, localized loss of capillaries with resulting ischemia, and, in the final stage of DR, the growth of abnormal ..
  44. Interventions Against the Molecular Etiology of BMPR2-induced PAH
    James D West; Fiscal Year: 2013
    ..Aberrant glucocorticoid receptor signaling leads to increased vascular adhesion and permeability by monocytes, and this in combination with increased ROS may drive recruitment of monocytes and alternative (M2)..
  45. Mitochondrial Protection Within Chronically Ischemic Myocardium
    Edward O McFalls; Fiscal Year: 2010
    ..anoxia-reoxygenation, by reducing superoxide generation and preventing a pathway that leads to mitochondrial permeability transition pore formation and cytochrome c loss...
  46. Mechanisms of endothelial barrier phenotypes in sickle cell disease
    SOLOMON FIIFI OFORI-ACQUAH; Fiscal Year: 2013
    ..We hypothesize that increased permeability of the vascular endothelium is a disorder of SCD that has not previously been appreciated...
  47. Matrix Otopathology
    MICHAEL ANNE NMI GRATTON; Fiscal Year: 2011
    ..and cell maturation, while in mature tissue they contribute to processes of cell adhesion, polarization and permeability. The role of basement membranes in the mature cochlea remains to be fully described...
  48. Novel Therapeutics for Neurotropic Alphaviruses
    David J Miller; Fiscal Year: 2013
    ..to enhance potency, reduce toxicity, improve solubility and metabolic stability, and optimize membrane permeability;(2) identify molecular target(s) responsible for their antiviral activity;(3) analyze the in vivo ..
  49. Treatment of stroke with a clinically approved proteasome inhibitor
    Li Zhang; Fiscal Year: 2012
    ..effectively reduces cerebral infarction, and concomitantly reduces secondary thrombosis and microvascular permeability in young rats...
  50. Chemical approaches for studying the biology of CD38
    Hening Lin; Fiscal Year: 2013
    ..These CD38 probes have unique features, such as cell permeability, compatibility with ligand binding to CD38, and the ability to inhibit CD38 enzymatic activity...
  51. Novel therapeutics for vesicants and toxic inhaled chemicals (U54)
    Carl W White; Fiscal Year: 2013
    ..Endpoints of oxidative stress, inflammation, increased permeability, and, for lungs, airway obstruction, will be evaluated...
  52. In vivo targeted gene silencing, a novel method
    WILLIAM ANTHONY TRUITT; Fiscal Year: 2012
    ..diagnostic and therapeutic agents, which to date have been limited for in vivo use due to poor membrane permeability of PNAs...
  53. Structure and function of chloride channels and transporters
    Alessio Accardi; Fiscal Year: 2013
    ..will test this hypothesis by manipulating through mutagenesis of these residues the selectivity of binding and permeability in order to alter the substrate specificity of the CLC channels and transporters...
  54. Targeting ATAP-mediated Apoptosis in Treatment of Prostate Cancer
    Zelin Sheng; Fiscal Year: 2013
    ..peptide can be effectively synthesized, and similar to the native ATAP, ATAP-iRGD can target mitochondria permeability for induction of apoptosis...
  55. Astrocytes Play a Critical Role in the Pathology of EAE
    JOHN ROLAND BETHEA; Fiscal Year: 2013
    ..Specifically, studies in Aim 2 will determine if there are differences in blood brain permeability and infiltration of leukocytes in the CNS of diseased WT and mutant mice...
  56. COMBINATORIAL-DESIGNED NANO-PLATFORMS TO OVERCOME TUMOR DRUG RESISTANCE
    Zhenfeng Duan; Fiscal Year: 2013
    ..a consequence of many factors that include poor systemic drug delivery efficiency, short residence time, poor permeability in tumor mass and intracellular availability, as well as microenvironmental selection pressures that give rise ..
  57. Neuroimmune modulation across the BBB: Regulation of ILS trafficking by TNF
    Weihong Pan; Fiscal Year: 2012
    ..perfusion studies in receptor knockout and wildtype mice, determine the effects of TNF on the transcellular permeability of IL15 in primary cerebral microvessel endothelial cells cultured in the Transwell system, and determine the ..
  58. Alcoholic Liver Dysfunction Potentiation by Hyperlipidemia and Cigarette Smoke
    SHANNON MARIE BAILEY; Fiscal Year: 2013
    ..alcohol, cigarette smoke, and hyperlipidemia increase mitochondrial dysfunction and promote the mitochondrial permeability transition;(2) Determine the individual and combined effects of alcohol, cigarette smoke, and hyperlipidemia on ..
  59. Regulatory targets and pathogenic mechanisms of anthrax edema factor
    Jin Mo Park; Fiscal Year: 2011
    ..VEGF is known to increase vascular permeability, thereby causing edema, and also promote the growth of lymphatic and blood vessels...
  60. TNF and Mitochondrial Dysfunction in ALD
    Craig J McClain; Fiscal Year: 2010
    ..We postulate that chronic alcohol abusecauses increased gut permeability and endotoxemia(increased LPS), depletionof critical antioxidants (e.g...
  61. Protein Kinase C in the Repair of Cellular Functions
    Grazyna Nowak; Fiscal Year: 2013
    ..by phosphorylating key proteins of mitochondrial oxidative phosphorylation apparatus and/or the mitochondrial permeability transition pore. The following specific aims will test this hypothesis...
  62. ANDV Induced Responses of Hypoxic Endothelial Cells
    Erich R Mackow; Fiscal Year: 2012
    ..ANDV infection of ECs provides a primary means for hantaviruses to alter vascular permeability. Although ECs are not lysed by ANDV infection, ECs line capillaries of vast pulmonary alveolar beds and ..
  63. Colon Barrier Breakdown in the Elderly
    James Mullin; Fiscal Year: 2005
    ..We have published numerous evidence that increased permeability in specifically epithelial tight junctions (a major parameter of epithelial barriers) is a promotional event in ..
  64. Human Blood-Brain Barrier Model for CNS Drug Translation
    Eric V Shusta; Fiscal Year: 2010
    ..cell sources amenable to high throughput assessment of central nervous system (CNS) therapeutics for their BBB permeability, a key criterion in the translation of new brain therapeutics to the clinic...
  65. LUNG CAPILLARY PERMEABILITY WITH HIGH VASCULAR PRESSURES
    Mary Townsley; Fiscal Year: 1991
    ..the capillary filtration coefficient and the osmotic reflection coefficient, which describe the capillary permeability to fluid and proteins respectively, over a wide range of pulmonary microvascular permeabilities, to correlate ..
  66. G Proteins and Endothelial Barrier Regulation
    Tatyana Voyno Yasenetskaya; Fiscal Year: 2004
    Loss of endothelial barrier integrity leads to increased vascular permeability and alveolar flooding, and contributes to morbidity and mortality associated with ARDS...
  67. TNFGAMMA EFFECTS ON BLOOD BRAIN BARRIER PERMEABILITY
    Donald Miller; Fiscal Year: 2001
    ..target for TNF-a is the endothelium, where increases in coagulant activity, cell adhesiveness and vascular permeability are observed...
  68. Development of a high-throughput screen for inhibitors of water permeability thro
    Marc Pelletier; Fiscal Year: 2009
    ..the upregulation of AQP4 in the aforementioned pathologies may have the beneficial effect of maximizing O2 permeability across the BBB, with the side effect of increasing osmotic water permeability and predisposing to cerebral ..
  69. Caveolin-1 and NO Regulate PMN-mediated Increases in Vascular Permeability
    Richard D Minshall; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Increased lung vascular endothelial permeability induced by activation of polymorphonuclear leukocytes (PMNs) adherent to endothelial cells via ICAM-1 leads to protein-rich pulmonary edema formation ..
  70. PERMEABILITY OF MORPHINE ACROSS BBB DURING DEVELOPMENT
    Anil Gulati; Fiscal Year: 1993
    ..It is not known how the morphine permeability across the BBB changes during development In the present proposal we plan to study the permeability of morphine ..
  71. Enhanced Drug Delivery to Metastatic Brain Tumors
    Keith Black; Fiscal Year: 2006
    ..increase drug delivery across the BTB, and have postulated the biochemical mechanisms of this selective BTB permeability increase...