quaternary protein structure

Summary

Summary: The characteristic 3-dimensional shape and arrangement of multimeric proteins (aggregates of more than one polypeptide chain).

Top Publications

  1. ncbi X-ray structure of a protein-conducting channel
    Bert van den Berg
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 427:36-44. 2004
  2. ncbi Crystal structure of the extracellular segment of integrin alpha Vbeta3 in complex with an Arg-Gly-Asp ligand
    Jian Ping Xiong
    Renal Unit, Leukocyte Biology and Inflammation Program, Structural Biology Program, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 02129, USA
    Science 296:151-5. 2002
  3. pmc Assembly reflects evolution of protein complexes
    Emmanuel D Levy
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nature 453:1262-5. 2008
  4. ncbi Structure of the dengue virus envelope protein after membrane fusion
    Yorgo Modis
    Howard Hughes Medical Institute, Children s Hospital and Harvard Medical School, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 427:313-9. 2004
  5. ncbi Crystal structure of Arp2/3 complex
    R C Robinson
    Structural Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 294:1679-84. 2001
  6. pmc Diversity of protein-protein interactions
    Irene M A Nooren
    European Bioinformatics Institute EMBL EBI, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK
    EMBO J 22:3486-92. 2003
  7. pmc Dimer ribbons of ATP synthase shape the inner mitochondrial membrane
    Mike Strauss
    Department of Structural Biology, Max Planck Institute of Biophysics, Frankfurt am Main, Germany
    EMBO J 27:1154-60. 2008
  8. pmc The structural basis of actin filament branching by the Arp2/3 complex
    Isabelle Rouiller
    Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    J Cell Biol 180:887-95. 2008
  9. ncbi Conserved arginine residues implicated in ATP hydrolysis, nucleotide-sensing, and inter-subunit interactions in AAA and AAA+ ATPases
    Teru Ogura
    Division of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 862 0976, Japan
    J Struct Biol 146:106-12. 2004
  10. ncbi Assembly and function of a bacterial genotoxin
    Dragana Nesic
    Laboratory of Structural Microbiology, The Rockefeller University, New York 10021, USA
    Nature 429:429-33. 2004

Detail Information

Publications308 found, 100 shown here

  1. ncbi X-ray structure of a protein-conducting channel
    Bert van den Berg
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 427:36-44. 2004
    ....
  2. ncbi Crystal structure of the extracellular segment of integrin alpha Vbeta3 in complex with an Arg-Gly-Asp ligand
    Jian Ping Xiong
    Renal Unit, Leukocyte Biology and Inflammation Program, Structural Biology Program, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 02129, USA
    Science 296:151-5. 2002
    ..Ligand binding induces small changes in the orientation of alphaV relative to beta3...
  3. pmc Assembly reflects evolution of protein complexes
    Emmanuel D Levy
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nature 453:1262-5. 2008
    ..Our model of self-assembly allows reliable prediction of evolution and assembly of a complex solely from its crystal structure...
  4. ncbi Structure of the dengue virus envelope protein after membrane fusion
    Yorgo Modis
    Howard Hughes Medical Institute, Children s Hospital and Harvard Medical School, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 427:313-9. 2004
    ..Specific features of the folded-back structure suggest strategies for inhibiting flavivirus entry...
  5. ncbi Crystal structure of Arp2/3 complex
    R C Robinson
    Structural Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 294:1679-84. 2001
    ..ARPC3 p21 and ARPC5 p16 are globular alpha-helical subunits. We predict that WASp/Scar proteins activate Arp2/3 complex by bringing Arp2 into proximity with Arp3 for nucleation of a branch on the side of a preexisting actin filament...
  6. pmc Diversity of protein-protein interactions
    Irene M A Nooren
    European Bioinformatics Institute EMBL EBI, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK
    EMBO J 22:3486-92. 2003
    ..Structural characteristics of different types of PPIs are discussed and related to their physiological function, specificity and evolution...
  7. pmc Dimer ribbons of ATP synthase shape the inner mitochondrial membrane
    Mike Strauss
    Department of Structural Biology, Max Planck Institute of Biophysics, Frankfurt am Main, Germany
    EMBO J 27:1154-60. 2008
    ..We propose that the mitochondrial ATP synthase organises itself into dimer ribbons to optimise its own performance...
  8. pmc The structural basis of actin filament branching by the Arp2/3 complex
    Isabelle Rouiller
    Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    J Cell Biol 180:887-95. 2008
    ..These data provide a rationale for why branch formation requires cooperative interactions among the Arp2/3 complex, nucleation-promoting factors, an actin monomer, and the mother filament...
  9. ncbi Conserved arginine residues implicated in ATP hydrolysis, nucleotide-sensing, and inter-subunit interactions in AAA and AAA+ ATPases
    Teru Ogura
    Division of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 862 0976, Japan
    J Struct Biol 146:106-12. 2004
    ..In this review, we will discuss what is known about these critical arginines and what can be concluded about their role in the function of AAA and AAA+ proteins...
  10. ncbi Assembly and function of a bacterial genotoxin
    Dragana Nesic
    Laboratory of Structural Microbiology, The Rockefeller University, New York 10021, USA
    Nature 429:429-33. 2004
    ..The holotoxin possesses a steric block of the CdtB active site by means of a non-globular extension of the CdtC subunit, and we identify putative DNA binding residues in CdtB that are essential for toxin activity...
  11. pmc Crystal structure of the extracellular segment of integrin alpha Vbeta3
    J P Xiong
    Renal Unit, Leukocyte Biology and Inflammation Program, Structural Biology Program, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, Charlestown, MA 02129, USA
    Science 294:339-45. 2001
    ..MIDAS lies adjacent to a calcium-binding site with a potential regulatory function...
  12. ncbi Refined structure of the nicotinic acetylcholine receptor at 4A resolution
    Nigel Unwin
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Mol Biol 346:967-89. 2005
    ..Access to the pore on the intracellular side is further influenced by narrow lateral windows, which would be expected to screen out electrostatically ions of the wrong charge and size...
  13. pmc Variant-specific [PSI+] infection is transmitted by Sup35 polymers within [PSI+] aggregates with heterogeneous protein composition
    Sviatoslav N Bagriantsev
    Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA
    Mol Biol Cell 19:2433-43. 2008
    ..Hsp104, Sis1, and Sse1 interact preferentially with the prion versus nonprion form of Sup35, whereas Sla2 and Ssb1/2 interact with both forms of Sup35 with similar efficiency...
  14. pmc Structure and mechanism of the M2 proton channel of influenza A virus
    Jason R Schnell
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 451:591-5. 2008
    ..Drug-resistance mutations are predicted to counter the effect of drug binding by either increasing the hydrophilicity of the pore or weakening helix-helix packing, thus facilitating channel opening...
  15. pmc Disulfide bridge formation between SecY and a translocating polypeptide localizes the translocation pore to the center of SecY
    Kurt S Cannon
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 169:219-25. 2005
    ..This suggests that the channel makes only limited contact with a translocating polypeptide, thus minimizing the energy required for translocation...
  16. ncbi Mechanisms of ataxin-3 misfolding and fibril formation: kinetic analysis of a disease-associated polyglutamine protein
    Andrew M Ellisdon
    Department of Biochemistry and Molecular Biology, PO Box 13D Monash University, 3800, Australia
    J Mol Biol 368:595-605. 2007
    ..These results enhance our general understanding of polyglutamine fibrillogenesis and highlights the role of non-poly(Q) domains in modulating the kinetics of misfolding in this family...
  17. pmc Assembly properties of the human immunodeficiency virus type 1 CA protein
    Barbie K Ganser-Pornillos
    Department of Biochemistry, University of Utah, Salt Lake City, Utah 84132, USA
    J Virol 78:2545-52. 2004
    ..In further support of this model, we demonstrate that the considerable morphological variation seen for conical HIV-1 capsids can be recapitulated in idealized capsid models by altering the distribution of pentamers...
  18. pmc Analysis of in-vivo LacR-mediated gene repression based on the mechanics of DNA looping
    Yongli Zhang
    Departments of Chemistry and Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, United States of America
    PLoS ONE 1:e136. 2006
    ....
  19. ncbi Peptide-based inhibitors of amyloid assembly
    Kimberly L Sciarretta
    The University of Chicago, Department of Pathology, Chicago, IL, USA
    Methods Enzymol 413:273-312. 2006
    ..Finally, we consider potential applications of inhibitor peptides to therapeutic strategies...
  20. pmc The protective antigen component of anthrax toxin forms functional octameric complexes
    Alexander F Kintzer
    Department of Chemistry, University of California, Berkeley, 94720, USA
    J Mol Biol 392:614-29. 2009
    ..This assembly mechanism may provide a novel means to control cytotoxicity...
  21. ncbi Rebuilt AAA + motors reveal operating principles for ATP-fuelled machines
    Andreas Martin
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 437:1115-20. 2005
    ..Energy-dependent machines with highly diverse quaternary architectures and molecular functions could operate by similar asymmetric mechanisms...
  22. pmc Structure of PolC reveals unique DNA binding and fidelity determinants
    Ronald J Evans
    Replidyne, Inc, Louisville, CO 80027, USA
    Proc Natl Acad Sci U S A 105:20695-700. 2008
    ..This ternary complex of a cellular replicative polymerase affords insights into polymerase fidelity, evolution, and structural diversity...
  23. ncbi Comparison of bacteriophage T4 UvsX and human Rad51 filaments suggests that RecA-like polymers may have evolved independently
    S Yang
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908 0733, USA
    J Mol Biol 312:999-1009. 2001
    ..This suggests that helical filaments in the RecA/Rad51/RadA family may have arisen from convergent evolution, with a conserved core structure that has assembled into multimeric filaments in a number of different ways...
  24. ncbi Structure of an atypical orphan response regulator protein supports a new phosphorylation-independent regulatory mechanism
    Eunmi Hong
    Department of Biochemistry and HTSD NMR and Application NRL, College of Science, Yonsei University, 134 Shinchon dong, Seodaemoon Gu, Seoul 120 749, Korea
    J Biol Chem 282:20667-75. 2007
    ..Our findings reveal how a new type of response regulator protein could function as a cell growth-associated regulator in the absence of post-translational modification...
  25. ncbi Structure of bovine mitochondrial F(1)-ATPase with nucleotide bound to all three catalytic sites: implications for the mechanism of rotary catalysis
    R I Menz
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Cell 106:331-41. 2001
    ..A catalytic scheme for hydrolysis (and synthesis) at physiological rates and a mechanism for the ATP-driven rotation of the gamma subunit are proposed based on the crystal structures of the bovine enzyme...
  26. ncbi Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel
    Randal B Bass
    Division of Chemistry and Chemical Engineering, Biochemistry Option, Howard Hughes Medical Institute, Mail Code 114 96, California Institute of Technology, Pasadena, CA 91125, USA
    Science 298:1582-7. 2002
    ..Although MscS is likely to be structurally distinct from other ion channels, similarities in gating mechanisms suggest common structural elements...
  27. ncbi Dissecting the interaction network of multiprotein complexes by pairwise coexpression of subunits in E. coli
    S Fribourg
    , CNRS/INSERM/ULP, , BP 163, Cedex C.U. de Strasbourg, Illkirch, 67404, France
    J Mol Biol 306:363-73. 2001
    ..Coexpression can be used as an alternative or complementary approach to conventional techniques for interaction studies such as yeast two-hybrid analysis, GST pulldown and immunoprecipitation...
  28. pmc Allostery and cooperativity revisited
    Qiang Cui
    Department of Chemistry and Theoretical Chemistry Institute, University of Wisconsin, Madison, Wisconsin 53706, USA
    Protein Sci 17:1295-307. 2008
    ..The presentation offers not only an up-to-date description of allostery from a theoretical/computational perspective, but also helps to resolve several outstanding issues concerning allostery...
  29. pmc Structural basis for tetraspanin functions as revealed by the cryo-EM structure of uroplakin complexes at 6-A resolution
    Guangwei Min
    Department of Biochemistry, New York University School of Medicine, New York, NY 10016, USA
    J Cell Biol 173:975-83. 2006
    ..The rod-shaped tetraspanin structure allows it to serve as stable pilings in the lipid sea, ideal for docking partner proteins to form structural/signaling networks...
  30. ncbi Structural diversity of ex vivo amyloid fibrils studied by cryo-electron microscopy
    J L Jimenez
    Department of Crystallography, Birkbeck College, Malet Street, London, WC1E 7HX, UK
    J Mol Biol 311:241-7. 2001
    ..We conclude that ex vivo amyloid fibrils, although variable, assemble as characteristic structures according to the identity of the precursor protein...
  31. ncbi Structural basis for allostery in integrins and binding to fibrinogen-mimetic therapeutics
    Tsan Xiao
    The CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 432:59-67. 2004
    ..Allostery in the head thus disrupts interaction between the legs in a previously described low-affinity bent integrin conformation, and leg extension positions the high-affinity head far above the cell surface...
  32. ncbi Crystal structure of hyaluronidase, a major allergen of bee venom
    Z Markovic-Housley
    Division of Structural Biology Biozentrum University of Basel CH 4056, Basel, Switzerland
    Structure 8:1025-35. 2000
    ..On the basis of sequence similarity, mammalian enzymes and Hya are assigned to glycosidase family 56 for which no structure has been reported yet...
  33. ncbi Structure shows that a glycosaminoglycan and protein recognition site in factor H is perturbed by age-related macular degeneration-linked single nucleotide polymorphism
    Andrew P Herbert
    Edinburgh Biomolecular NMR Unit, School of Chemistry and School of Biological Sciences, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, Scotland, United Kingdom
    J Biol Chem 282:18960-8. 2007
    ....
  34. ncbi Identification of novel interactions in HIV-1 capsid protein assembly by high-resolution mass spectrometry
    Jason Lanman
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294 2170, USA
    J Mol Biol 325:759-72. 2003
    ..Most significantly, we have identified a previously unrecognized intersubunit N domain-C domain interaction. The detection of this interaction reconciles previously discrepant biophysical and genetic data...
  35. ncbi The Atx1-Ccc2 complex is a metal-mediated protein-protein interaction
    Lucia Banci
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Nat Chem Biol 2:367-8. 2006
    ..Copper was required for the interaction. This study provides an understanding of metal-mediated protein-protein interactions in which the metal ion is essential for the weak, reversible interaction between the partners...
  36. ncbi Structure of the mammalian mitochondrial ribosome reveals an expanded functional role for its component proteins
    Manjuli R Sharma
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201, USA
    Cell 115:97-108. 2003
    ....
  37. ncbi Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution
    P Cramer
    Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305-5126, USA
    Science 292:1863-76. 2001
    ..The results also provide evidence for RNA exit in the vicinity of the carboxyl-terminal repeat domain, coupling synthesis to RNA processing by enzymes bound to this domain...
  38. ncbi Dependence of alpha-synuclein aggregate morphology on solution conditions
    Wolfgang Hoyer
    Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    J Mol Biol 322:383-93. 2002
    ....
  39. pmc Coupling between voltage sensors and activation gate in voltage-gated K+ channels
    Zhe Lu
    Department of Physiology, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Gen Physiol 120:663-76. 2002
    ..One sequence is the so called S4-S5 linker distal to the voltage-sensing S4, while the other is around the COOH-terminal end of S6, a region containing the actual gate-forming residues...
  40. ncbi Structure of full-length HIV-1 CA: a model for the mature capsid lattice
    Barbie K Ganser-Pornillos
    The Scripps Research Institute, Department of Cell Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 131:70-9. 2007
    ..Interactions between the two domains of CA further stabilize the hexamer and provide a structural explanation for the mechanism of action of known HIV-1 assembly inhibitors...
  41. ncbi Prions affect the appearance of other prions: the story of [PIN(+)]
    I L Derkatch
    Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, 900 South Ashland Avenue, Chicago, IL 60607, USA
    Cell 106:171-82. 2001
    ..Importantly, the de novo appearance of Rnq1::GFP prion aggregates also requires the presence of other prions, suggesting the existence of a general mechanism by which the appearance of prions is enhanced by heterologous prion aggregates...
  42. pmc Design of therapeutic proteins with enhanced stability
    Naresh Chennamsetty
    Massachusetts Institute of Technology, Chemical Engineering, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 106:11937-42. 2009
    ..The technology described here could be used to incorporate developability in a rational way during the screening of antibodies in the discovery phase for several diseases...
  43. ncbi Abeta(1-40) fibril polymorphism implies diverse interaction patterns in amyloid fibrils
    Jessica Meinhardt
    Leibniz Institut für Altersforschung Fritz Lipmann Institut, Beutenbergstrabetae 11, D 07745 Jena, Germany
    J Mol Biol 386:869-77. 2009
    ..This property differs significantly from native, monomeric protein folding reactions that produce, for one protein sequence, only one ordered conformation and only one set of inter-residue interactions...
  44. ncbi Evidence for macromolecular protein rings in the absence of bulk water
    Brandon T Ruotolo
    Department of Chemistry, Lensfield Road, University of Cambridge, Cambridge CB2 1EW, UK
    Science 310:1658-61. 2005
    ....
  45. ncbi Electron cryo-microscopy shows how strong binding of myosin to actin releases nucleotide
    Kenneth C Holmes
    Department of Biophysics, Max Planck Institute for Medical Research, 69120 Heidelberg, Germany
    Nature 425:423-7. 2003
    ..The closing of the actin-binding cleft when actin binds is structurally coupled to the opening of the nucleotide-binding pocket...
  46. ncbi Mapping the core of the beta(2)-microglobulin amyloid fibril by H/D exchange
    Masaru Hoshino
    Institute for Protein Research, Osaka University, Yamadaoka 3 2, Suita, Osaka 565 0871, Japan
    Nat Struct Biol 9:332-6. 2002
    ..The present method could be used to obtain residue-specific conformational information of various amyloid fibrils, even though it does not provide a high resolution three-dimensional structure...
  47. ncbi Structure of the autoinhibited kinase domain of CaMKII and SAXS analysis of the holoenzyme
    Oren S Rosenberg
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell 123:849-60. 2005
    ....
  48. pmc Visualizing the ATPase cycle in a protein disaggregating machine: structural basis for substrate binding by ClpB
    Sukyeong Lee
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Mol Cell 25:261-71. 2007
    ..Taken together, our results support a mechanism by which ClpB captures substrates on the upper surface of the AAA-1 ring before threading them through the ClpB hexamer in an ATP hydrolysis-driven step...
  49. pmc Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunits
    R N Gunawardane
    Howard Hughes Medical Institute, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
    J Cell Biol 151:1513-24. 2000
    ..We found that Dgrips128 and 163 can each interact with gammaTuSC. However, this interaction is insufficient for gammaTuRC assembly...
  50. pmc A ligand-binding pocket in the dengue virus envelope glycoprotein
    Yorgo Modis
    Howard Hughes Medical Institute, Children s Hospital and Harvard Medical School, 320 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:6986-91. 2003
    ..These features point to a structural pathway for the fusion-activating transition and suggest a strategy for finding small-molecule inhibitors of dengue and other flaviviruses...
  51. ncbi Methods to monitor the quaternary structure of G protein-coupled receptors
    Graeme Milligan
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, UK
    FEBS J 272:2914-25. 2005
    ..Herein we assess the widely applied techniques and discuss the relative benefits and limitations of these approaches...
  52. pmc Helical extension of the neuronal SNARE complex into the membrane
    Alexander Stein
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Nature 460:525-8. 2009
    ..Our results suggest that the final phase of SNARE assembly is directly coupled to membrane merger...
  53. pmc Cleaved antitrypsin polymers at atomic resolution
    M A Dunstone
    The Ian Potter Foundation Protein Crystallography Laboratory, St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    Protein Sci 9:417-20. 2000
    ..Here, we describe the first crystallographic evidence of a beta-strand linked polymer form of alpha1-antitrypsin: the crystal structure of a cleaved alpha1-antitrypsin polymer...
  54. ncbi Hsp104 targets multiple intermediates on the amyloid pathway and suppresses the seeding capacity of Abeta fibrils and protofibrils
    Muriel Arimon
    Laboratory of Molecular Neurobiology and Neuroproteomics, Swiss Federal Institute of Technology Lausanne EPFL, FSV BMI AI 2137 1, Station 15, CH 1015 Lausanne, Switzerland
    J Mol Biol 384:1157-73. 2008
    ..Together, these findings suggest that the strong inhibition of Abeta fibrillization by Hsp104 is mediated by its ability to act at different stages and target multiple intermediates on the pathway to amyloid formation...
  55. ncbi Cooperative hemoglobins: conserved fold, diverse quaternary assemblies and allosteric mechanisms
    W E Royer
    Dept of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Trends Biochem Sci 26:297-304. 2001
    ..This mechanistic diversity, which occurs between assemblies of subunits that have the same fold, provides insight into the range of regulatory strategies that are available to protein molecules...
  56. pmc The role of Xgrip210 in gamma-tubulin ring complex assembly and centrosome recruitment
    L Zhang
    Howard Hughes Medical Institute, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
    J Cell Biol 151:1525-36. 2000
    ..These results suggest that Xgrip210 is a component of the gammaTuRC cap structure that is required for the assembly of the gammaTuRC...
  57. ncbi Analysis of the native quaternary structure of vanilloid receptor 1
    N Kedei
    Laboratory of Cellular Carcinogenesis and Tumor Promotion, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:28613-9. 2001
    ..Finally, differences in glycosylation between the systems were observed, indicating the need for caution in the use of the heterologous expression systems for analysis of VR1 properties...
  58. pmc On the mechanism of sensing unfolded protein in the endoplasmic reticulum
    Joel J Credle
    Howard Hughes Medical Institute, Departments of Biochemistry and Biophysics and Medicine, University of California, San Francisco, CA 94143 2200, USA
    Proc Natl Acad Sci U S A 102:18773-84. 2005
    ..The changes in the ER lumen in turn position Ire1 kinase domains in the cytoplasm optimally for autophosphorylation to initiate the UPR...
  59. pmc Experimental constraints on quaternary structure in Alzheimer's beta-amyloid fibrils
    Aneta T Petkova
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0520, USA
    Biochemistry 45:498-512. 2006
    ..These models apply to Abeta(1-40) fibrils grown with gentle agitation. We also present evidence for different internal quaternary contacts in Abeta(1-40) fibrils grown without agitation, which are morphologically distinct...
  60. ncbi Crystal structure of the human nuclear cap binding complex
    C Mazza
    European Molecular Biology Laboratory, Grenoble Outstation, 6 rue Jules Horowitz, BP181, F 38042 9, Grenoble Cedex, France
    Mol Cell 8:383-96. 2001
    ..Site-directed mutagenesis revealed 4 residues of CBP20 which are critical for cap binding. A model for cap binding is proposed based on these results and the known mode of binding of RNA to RNP domains...
  61. ncbi Structural basis of DNA recognition by p53 tetramers
    Malka Kitayner
    Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100
    Mol Cell 22:741-53. 2006
    ....
  62. ncbi Cholesterol, a modulator of membrane-associated Abeta-fibrillogenesis and neurotoxicity
    C M Yip
    Institute for Biomaterials and Biomedical Engineering and Centre for Studies in Molecular Imaging, University of Toronto, Ontario, Canada
    J Mol Biol 311:723-34. 2001
    ..These results collectively suggest that Abeta-cell surface interactions are mediated by cellular cholesterol levels, the distribution of cholesterol throughout the cell, and membrane fluidity...
  63. ncbi Structure of the tetraspanin main extracellular domain. A partially conserved fold with a structurally variable domain insertion
    M Seigneuret
    Unite Mixte de Recherche, 7033 CNRS, Laboratoire de Physicochimie Biomoléculare et Cellulaire, Universite Paris 6, 4 place Jussieu, 75005 Paris, France
    J Biol Chem 276:40055-64. 2001
    ..The tetraspanin EC2 seems to correspond to a new scheme of fold conservation/variability among proteins, namely the insertion of a structurally variable subdomain within an otherwise conserved fold...
  64. ncbi Transthyretin aggregation under partially denaturing conditions is a downhill polymerization
    Amy R Hurshman
    Department of Chemistry and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road BCC 506, La Jolla, California 92037, USA
    Biochemistry 43:7365-81. 2004
    ....
  65. pmc Tetrameric structure of centromeric nucleosomes in interphase Drosophila cells
    Yamini Dalal
    Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America
    PLoS Biol 5:e218. 2007
    ..This demonstration of stable half-nucleosomes in vivo provides a possible basis for the instability of centromeric nucleosomes that are deposited in euchromatic regions, which might help maintain centromere identity...
  66. ncbi Structure of the uncleaved human H1 hemagglutinin from the extinct 1918 influenza virus
    James Stevens
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 303:1866-70. 2004
    ....
  67. ncbi Cys-loop receptors: new twists and turns
    Henry A Lester
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Trends Neurosci 27:329-36. 2004
    ....
  68. pmc Structural and dynamic aspects related to oligomerization of apo SOD1 and its mutants
    Lucia Banci
    Magnetic Resonance Center and Department of Chemistry, Fiorgen Foundation, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy
    Proc Natl Acad Sci U S A 106:6980-5. 2009
    ..The present results suggest that the investigation of the solution state coupled with that of the crystal state can provide major insights into SOD1 pathway toward oligomerization in relation to fALS...
  69. pmc Crystal structure of calpain reveals the structural basis for Ca(2+)-dependent protease activity and a novel mode of enzyme activation
    C M Hosfield
    Department of Biochemistry, Queen s University and The Protein Engineering Network of Centres of Excellence, Kingston, Ontario, Canada K7L 3N6
    EMBO J 18:6880-9. 1999
    ..This Ca(2+)-dependent activation mechanism is clearly distinct from those of classical proteases...
  70. ncbi Mechanism of DNA translocation in a replicative hexameric helicase
    Eric J Enemark
    W M Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 442:270-5. 2006
    ..These events permute sequentially around the ring from one subunit to the next...
  71. ncbi Mechanism of glutamate receptor desensitization
    Yu Sun
    Department of Biochemistry and Molecular Biophysics, Columbia University, 650 West 168th Street, New York, New York 10032, USA
    Nature 417:245-53. 2002
    ..Desensitization occurs through rearrangement of the dimer interface, which disengages the agonist-induced conformational change in the ligand-binding core from the ion channel gate...
  72. pmc Structural insights into a yeast prion illuminate nucleation and strain diversity
    Rajaraman Krishnan
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 435:765-72. 2005
    ..These findings resolve several problems in yeast prion biology and have broad implications for other amyloids...
  73. pmc Discovery of CD8+ T cell epitopes in Chlamydia trachomatis infection through use of caged class I MHC tetramers
    Gijsbert M Grotenbreg
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 105:3831-6. 2008
    ....
  74. pmc Dimerisation-dependent GTPase reaction of MnmE: how potassium acts as GTPase-activating element
    Andrea Scrima
    Max Planck Institut fur molekulare Physiologie, Dortmund, Germany
    EMBO J 25:2940-51. 2006
    ..We propose a model for the juxtaposition of G-domains in the full-length protein and how it induces conformational changes in the putative tRNA-modification centre...
  75. pmc Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c
    Christian Lange
    Max Planck Institute for Biophysics, Heinrich Hoffmann Strasse 7, D 60528 Frankfurt am Main, Germany
    Proc Natl Acad Sci U S A 99:2800-5. 2002
    ..Obviously, cytochrome c reduction by the cytochrome bc1 complex can be regulated in response to respiratory conditions...
  76. ncbi Structural determinants for generating centromeric chromatin
    Ben E Black
    Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, California 92093, USA
    Nature 430:578-82. 2004
    ..Thus, the centromere-targeting domain of CENP-A confers a unique structural rigidity to the nucleosomes into which it assembles, and is likely to have a role in maintaining centromere identity...
  77. ncbi The physical basis of how prion conformations determine strain phenotypes
    Motomasa Tanaka
    Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California San Francisco and California Institute for Quantitative Biomedical Research, San Francisco, California 94143, USA
    Nature 442:585-9. 2006
    ..The propensity of aggregates to undergo breakage, thereby generating new seeds, probably represents a key determinant of their physiological impact for both infectious (prion) and non-infectious amyloids...
  78. pmc Conformational changes in dynamin on GTP binding and oligomerization reported by intrinsic and extrinsic fluorescence
    Elena Solomaha
    Department of Neurobiology, Pharmacology and Physiology, University of Chicago, 947 E 58th St, Chicago, IL 60637, USA
    Biochem J 391:601-11. 2005
    ..In addition, energy transfer was observed when oligomerization was promoted in mixtures of IAEDANS.dynamin-2 and 4-(4-dimethylaminophenylazo)benzoic acid-coupled dynamin-2, an effect that was counteracted by GTP but not GDP...
  79. ncbi Structural mechanics of the pH-dependent activity of beta-carbonic anhydrase from Mycobacterium tuberculosis
    Adrian Suarez Covarrubias
    Department of Cell and Molecular Biology, Uppsala University, S 751 24 Uppsala, Sweden
    J Biol Chem 281:4993-9. 2006
    ..The data presented here, therefore, suggest a carboxylate shift on/off switch for the enzyme, which may, in turn, be controlled by a dimer-to-tetramer equilibrium...
  80. ncbi Structure and functional analysis of the fungal galectin CGL2
    Piers J Walser
    Institute of Microbiology, Swiss Federal Institute of Technology, Schmelzbergstrasse 7, CH 8092, Zurich, Switzerland
    Structure 12:689-702. 2004
    ..The geometric constraints due to the orthogonal orientation of the four binding sites have important consequences on substrate binding and selectivity...
  81. ncbi Crossbridge and tropomyosin positions observed in native, interacting thick and thin filaments
    R Craig
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Mol Biol 311:1027-36. 2001
    ....
  82. ncbi Crystal structure of an ACh-binding protein reveals the ligand-binding domain of nicotinic receptors
    K Brejc
    Division of Molecular Carcinogenesis, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Nature 411:269-76. 2001
    ..This AChBP structure is relevant for the development of drugs against, for example, Alzheimer's disease and nicotine addiction...
  83. pmc Association properties of betaB1- and betaA3-crystallins: ability to form heterotetramers
    May P Chan
    National Eye Institute and National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 47:11062-9. 2008
    ..These findings suggest a unique role for betaB1-crystallin in promoting higher-order crystallin association in the lens...
  84. pmc Structural determinants involved in the formation and activation of G protein betagamma dimers
    William E McIntire
    Department of Pharmacology, University of Virginia Health System, Charlottesville, VA 22908, USA
    Neurosignals 17:82-99. 2009
    ....
  85. pmc Three-dimensional EM structure of the ectodomain of integrin {alpha}V{beta}3 in a complex with fibronectin
    Brian D Adair
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 168:1109-18. 2005
    ..We conclude that the ectodomain of alphaVbeta3 manifests a bent conformation that is capable of stably binding a physiological ligand in solution...
  86. pmc Structural basis of chaperone recognition of type III secretion system minor translocator proteins
    Viviana Job
    Bacterial Pathogenesis Group, Institut de Biologie Structurale, UMR 5075, CNRS Commissariat à l Enérgie Atomique Université Joseph Fourier, 41 rue Jules Horowitz, 38027 Grenoble, France
    J Biol Chem 285:23224-32. 2010
    ..The PcrH-PopD complex displays homologs among a number of pathogenic strains and could represent a novel, potential target for antibiotic development...
  87. ncbi Structure and function of hexameric helicases
    S S Patel
    Department of Biochemistry, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Annu Rev Biochem 69:651-97. 2000
    ..Possible unwinding mechanisms based on the DNA exclusion model are proposed here, termed the wedge, torsional, and helix-destabilizing models...
  88. ncbi Functional domains in tetraspanin proteins
    Christopher S Stipp
    Dana Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Trends Biochem Sci 28:106-12. 2003
    ....
  89. ncbi It takes two to tango: regulation of G proteins by dimerization
    Raphael Gasper
    Max Planck Institut fur molekulare Physiologie, Abteilung Strukturelle Biologie, Dortmund, Germany
    Nat Rev Mol Cell Biol 10:423-9. 2009
    ..We propose that the juxtaposition of the G domains of two monomers across the GTP-binding sites activates the biological function of these proteins and the GTPase reaction...
  90. ncbi Molecular model for a complete clathrin lattice from electron cryomicroscopy
    Alexander Fotin
    Biophysics Graduate Program, Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 432:573-9. 2004
    ..These invariant local interactions appear to stabilize the lattice, allowing assembly and uncoating to be controlled by events at a few specific sites...
  91. ncbi Structure of the connexin 26 gap junction channel at 3.5 A resolution
    Shoji Maeda
    Institute for Protein Research, Osaka University, OLABB, 6 2 3, Furuedai, Suita, Osaka 565 0874, Japan
    Nature 458:597-602. 2009
    ..The structure of the Cx26 gap junction channel also has implications for the gating of the channel by the transjunctional voltage...
  92. ncbi Crystal structure of a complex between the catalytic and regulatory (RIalpha) subunits of PKA
    Choel Kim
    Department of Chemistry and Biochemistry, University of California, San Diego, CA 92093, USA
    Science 307:690-6. 2005
    ..The beta barrel of cAMP binding domain A, which is the docking site for cAMP, remains largely intact in the complex, whereas the helical subdomain undergoes major reorganization...
  93. ncbi Mechanisms and structures of crotonase superfamily enzymes--how nature controls enolate and oxyanion reactivity
    R B Hamed
    Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Mansfield Road, Oxford, OX1 3TA, UK
    Cell Mol Life Sci 65:2507-27. 2008
    ..The range and efficiency of known CS-catalyzed reactions coupled to their common structural platforms suggest that CS variants may have widespread utility in biocatalysis...
  94. pmc Prion species barrier between the closely related yeast proteins is detected despite coaggregation
    Buxin Chen
    School of Biology and Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 310 Ferst Drive, Atlanta, GA 30332 0230, USA
    Proc Natl Acad Sci U S A 104:2791-6. 2007
    ....
  95. pmc Conditional MHC class I ligands and peptide exchange technology for the human MHC gene products HLA-A1, -A3, -A11, and -B7
    Arnold H Bakker
    Divisions of Immunology and Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Proc Natl Acad Sci U S A 105:3825-30. 2008
    ..The conditional ligand technology developed here will allow high-throughput MHC-based analysis of cytotoxic T cell immunity in the vast majority of Western European individuals...
  96. pmc Mutational evidence for an internal fusion peptide in flavivirus envelope protein E
    S L Allison
    Institute of Virology, University of Vienna, A 1095 Vienna, Austria
    J Virol 75:4268-75. 2001
    ..These data support the hypothesis that the cd loop may be directly involved in interactions with target membranes during fusion...
  97. pmc Normal mode analysis suggests a quaternary twist model for the nicotinic receptor gating mechanism
    Antoine Taly
    Recepteurs et Cognition, Unité de Recherche Associeé URA Centre National de la Recherche Scientifique 2182, Institut Pasteur, Paris, France
    Biophys J 88:3954-65. 2005
    ....
  98. ncbi Subunit exchange, conformational stability, and chaperone-like function of the small heat shock protein 16.5 from Methanococcus jannaschii
    Michael P Bova
    Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, California 90095, USA
    J Biol Chem 277:38468-75. 2002
    ..The results of this study support the hypothesis that MjHsp16.5 has a dynamic quaternary structure at temperatures that are physiologically relevant to M. jannaschii...
  99. ncbi The E. coli BtuCD structure: a framework for ABC transporter architecture and mechanism
    Kaspar P Locher
    Howard Hughes Medical Institute and Division of Chemistry and Chemical Engineering, Mail Code 147 75CH, California Institute of Technology, Pasadena, CA 91125, USA
    Science 296:1091-8. 2002
    ..A prominent cytoplasmic loop of BtuC forms the contact region with the ATP-binding cassette and appears to represent a conserved motif among the ABC transporters...
  100. ncbi The power of two: protein dimerization in biology
    Neelan J Marianayagam
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW 2006, Australia
    Trends Biochem Sci 29:618-25. 2004
    ..Specific protein dimerization is integral to biological function, structure and control, and must be under substantial selection pressure to be maintained with such frequency throughout biology...
  101. pmc N-terminal domain of yeast Hsp104 chaperone is dispensable for thermotolerance and prion propagation but necessary for curing prions by Hsp104 overexpression
    Guo Chiuan Hung
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0851, USA
    Genetics 173:611-20. 2006
    ....

Research Grants65

  1. Capsular Assemblies Driven by the Hydrophobic Effect
    Bruce C Gibb; Fiscal Year: 2010
    ..of nano-capsules in aqueous solution;2) to use this information to both gain an understanding of quaternary protein structure, and devise self-assembling nano-capsules that function as drug delivery systems...
  2. STRUCTURAL BASIS FOR ASSEMBLY OF COOPERATIVE HEMOGLOBINS
    WILLIAM ROYER; Fiscal Year: 2005
    ..Our structural analysis suggests a number of hypotheses for the regulation of function, which we will be testing by mutagenesis, kinetic and structural experiments. ..
  3. Ultrafast Time-Resolved Crystallography on Scapharca Hb
    WILLIAM ROYER; Fiscal Year: 2007
    ..Understanding such principles will aid in the rational manipulation of a number of biological processes. ..
  4. Structural basis of regulation of IRF
    WILLIAM ROYER; Fiscal Year: 2007
    ..These studies will provide insight into the molecular mechanism of IRF signaling, providing possible targets for rational drug design to combat viral pathogenesis. ..
  5. Human mitochondrial fatty acid synthase
    Stuart Smith; Fiscal Year: 2009
    ..Elucidation of the role of the mitFAS system in mitochondrial function may aid in understanding the etiology of these disorders. ..
  6. Age-related structural alterations in loss of self-tolerance to a3(IV) collagen
    Dorin Bogdan Borza; Fiscal Year: 2007
    ..New targets for prevention or therapeutic intervention may be identified as a result. [unreadable] [unreadable] [unreadable] [unreadable]..
  7. Factor B and energy coupling in mitochondria
    GRIGORY BELOGRUDOV; Fiscal Year: 2007
    ..The proposed research is expected to advance the knowledge of the molecular mechanisms of energy transfer and conservation in mitochondria, and shed light on the role of mitochondria in a number of human disorders. ..
  8. Mapping the Crystallization Slot for Membrane Proteins(R
    PATRICK LOLL; Fiscal Year: 2006
    ..High resolution structural information is absolutely required for any in-depth understanding of the basic functioning of these molecules, as well as for the discovery and development of new therapeutic agents. ..
  9. Epithelial Na channel (ENaC) polymorphisms in hyptertention
    James Stockand; Fiscal Year: 2007
    ..Preliminary results support the feasibility of this investigation and suggest that this line of inquiry will yield significant and novel findings. [unreadable] [unreadable]..
  10. MOLECULAR MECHANISMS OF BACTERIAL PATHOGENESIS
    Robert Collier; Fiscal Year: 2007
    ..abstract_text> ..
  11. MHC AND GAMMA/DELTA T-CELL RECOGNITION
    Yueh Hsiu Chien; Fiscal Year: 2007
    ..abstract_text> ..
  12. Neuromodulation of Voltage-gated Ca2+ Channels
    Jian Yang; Fiscal Year: 2007
    ..This research may not only enhance our understanding of the complex regulatory pathways for VGCCs but also provide valuable information for the development of potential therapeutic reagents that target these pathways ..
  13. The mechanism of immunosuppression by thymosin beta 4.
    ELENA YARMOLA; Fiscal Year: 2007
    ..As an initial step toward these goals, I specifically propose to investigate the mechanism by which thymosin beta4 is oxidized, secreted, and recognized by cells of the immune system. ..
  14. Structural Determinants of Glycogen Initiation
    Thomas Hurley; Fiscal Year: 2006
    ..4) Test the proposed roles for specific residues in the active site of glycogenin during catalysis and for the recognition of substrate and acceptor molecules. [unreadable] [unreadable]..
  15. A screen for small molecule inhibitors of soluble Abeta oligomer assembly
    Harry LeVine; Fiscal Year: 2007
    ..Anti-oligomer compounds could potentially block the cognitive impairment symptoms that oligomers elicit in animal models and interfere with the progression of Alzheimer's disease. [unreadable] [unreadable] [unreadable]..
  16. A study of mechanosensation using microbial TRP channels
    Ching Kung; Fiscal Year: 2008
    ..Taking advantage of the facile and powerful genetic manipulations, we plan to use forward and reverse genetics to find the parts of the TrpY1 molecule that are important in mechanosensation. [unreadable] [unreadable] [unreadable]..
  17. Structural mechanism of DNA segregation by the pSK41 par system
    Maria Schumacher; Fiscal Year: 2010
    ..Thus, understanding the structural basis for DNA segregation by this par system will provide several points of potential therapeutic intervention against pSK41 harboring multidrug resistant S. aureus strains. ..
  18. The Structural Basis of Eukaryotic Replication Origin Licensing by Cryo-EM
    Huilin Li; Fiscal Year: 2010
    ..These detailed structural studies will provide a long-awaited molecular mechanism for the origin licensing stage of the intricate replication initiation process. ..
  19. Multi-Resolution Docking Methods for Electron Microscopy
    Willy Wriggers; Fiscal Year: 2009
    ..The algorithmic and methodological developments will be distributed freely through the established internet-based mechanisms employed for the Situs docking package. ..
  20. Regulation of TRAIL-induced apoptosis
    Francis Chan; Fiscal Year: 2009
    ..Our experiments may also provide a useful diagnostic tool to determine response of cancers to treatments involving TRAIL or agonist TRAIL antibodies. ..
  21. Molecular mechanisms of vertebrate Mg2+ homeostasis
    Andrew Scharenberg; Fiscal Year: 2009
    ..Together, the proposed studies will generate a foundation of knowledge on the molecular mechanisms of Mg uptake and their integration with mechanisms which regulate cell growth and proliferation. ..
  22. Stoichiometry and modular retrieval of ENaC
    James Stockand; Fiscal Year: 2009
    ..This research will provide critical insight about the molecualr architecture of ENaC and regulation of this important channel. ..
  23. Role of dynamin-auxilin interactions in endocytosis
    Sanja Sever; Fiscal Year: 2009
    ..The function of lipid binding/phosphatase activity by auxilin in endocytosis will be examined using a semi-permeable cell assay. ..
  24. Structure and Function of superantigens
    Hongmin Li; Fiscal Year: 2007
    ..The sum of these studies will contribute to our understanding of disease pathogenesis, and will provide new avenues for therapeutic intervention. ..
  25. Structural studies on the Initiator protein, IBP39.
    Maria Schumacher; Fiscal Year: 2008
    ..vaginalis suggests it is a possible target for the treatment of trichomoniasis, which afflicts over 170 million individuals each _,ear, ..
  26. STRUCTURE AND SPECIFICITY OF LIPOGENIC ENZYMES
    Stuart Smith; Fiscal Year: 2008
    ..abstract_text> ..
  27. X-ray Structure of Human Aldehyde Dehydrogenase
    Thomas Hurley; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  28. APP, Alzheimer's Disease, and Synaptic Function
    THOMAS SUDHOF; Fiscal Year: 2008
    ..Together these studies will contribute to our understanding of APP function and Abeta toxicity in AD, and may suggest new avenues for interfering with the pathogenesis of AD. ..
  29. The assembly of characterization of tau oligomers
    Michelle King; Fiscal Year: 2006
    ..Together, the aims of this study will facilitate a greater understanding of tau filaments and tau filament assembly in a number of neurodegenerative diseases including Alzheimer's Disease. ..
  30. Structural Basis of Novel Hookworm Vaccines
    Oluwatoyin Asojo; Fiscal Year: 2006
    ..56 Angstroms (protein data bank code 1U53). In addition, a 2 PR-1 domain ASP (Na-ASP-1) has been crystallized with data up to 2.7 Angstroms. [unreadable] [unreadable] [unreadable]..
  31. Structural investigation of Arp2/3 complex activation
    Bradley Nolen; Fiscal Year: 2006
    ..Furthermore, it will show how nucleation promoting factors activate the complex. This will provide a basis for a better understanding of cytoskeletal function in both normal and diseased states of the cell. ..
  32. Regulation of Tangential Migration in the Embryonic CNS
    Andrew Peterson; Fiscal Year: 2004
    ..abstract_text> ..
  33. Molecular Mechanisms of Aging: 33rd Ann. Mtg. of AGE
    Norman Wolf; Fiscal Year: 2004
    ..Dr. Bickford has investigated hotel accommodations there and has found that both the Radisson and the Hilton offer excellent accommodations for the meeting. She is negotiating with both. ..
  34. Electron Microscope for New Fitzsimons Facility
    Kathryn Howell; Fiscal Year: 2004
    ..The sixteen major users will pursue questions integral to their NIH funded research and the availability of this instrumentation will enhance their NIH funded research. ..
  35. FUNCTION AND STRUCTURE OF ANNEXIN
    James Hall; Fiscal Year: 2003
    ..2. Determin the topography and oligomerization state of annexin. 3. Investigate the channel-forming abilities of annexin. ..
  36. Directed evolution of P450 peroxide-mediated activity
    JONATHAN SILBERG; Fiscal Year: 2004
    ....
  37. HYPOXIA INDUCIBLE VEGF PRODUCTION IN SLEEP APNEA
    Andrew Levy; Fiscal Year: 2003
    ....
  38. SYNUCLEIN FUNCTIONS: ROLE IN PARKINSON'S DISEASE
    THOMAS SUDHOF; Fiscal Year: 2003
    ..We anticipate that these experiments will establish a molecular understanding of synuclein functions in the brain and provide insight into the role of an alpha synuclein in Parkinson's disease. ..
  39. MECHANISMS OF ORAL TOLERANCE
    Yueh Hsiu Chien; Fiscal Year: 2002
    ..The information may also be important for mucosal vaccine development. ..
  40. STRUCTURE AND MECHANISM OF PROSTAGLANDIN H2 SYNTHASE
    PATRICK LOLL; Fiscal Year: 2002
    ..Finally, structures of different cyclooxygenase or peroxidase deficient forms of the enzyme will be determined to help clarify the relationship between the two activities. ..
  41. MOLECULAR PHARMACOLOGY OF VOLTAGE SENSITIVE NA CHANNELS
    Edward Moczydlowski; Fiscal Year: 2001
    ..These biophysical studies will allow us deduce the functional contribution of key residues that determine ionic selectivity and unitary conductance of the NaV channel pore. ..
  42. Assembly of tau filaments in cultured cells
    Michelle King; Fiscal Year: 2004
    ..abstract_text> ..
  43. Crystallization of Ataxin-3
    PATRICK LOLL; Fiscal Year: 2005
    ..The molecular understanding of ataxin-3 that will be obtained from this work is a prerequisite for any rational design of therapeutics that will interfere with the protein's aberrant folding and aggregation. ..
  44. The mechanism of crosslinking by proteins that create adhesive gels
    Andrew Smith; Fiscal Year: 2006
    ..Once the remarkable natural adhesives used by animals are understood, it may be possible to design adhesives with similar properties that could be used in a wide variety of medical applications. [unreadable] [unreadable] [unreadable]..
  45. Structure Analysis of Aquaporin Membrane Channels
    Mark Yeager; Fiscal Year: 2006
    ..In addition, this project will provide insight into the molecular basis for channel regulation and will contribute to the general principles that govern conformational changes of membrane proteins. ..
  46. Expression and Structure of Soluble Human Nucleotidases
    TERENCE KIRLEY; Fiscal Year: 2006
    ..Second, it will demonstrate how two different protein primary structures are used to do the same enzymatic task, aiding in the design of more efficacious therapeutic soluble nucleotidases. ..
  47. Human endogenous retrovirus superantigen HERV-K18
    Hongmin Li; Fiscal Year: 2006
    ..abstract_text> ..
  48. Exercise Training in Insulin Resistant Non-Diabetics
    Ira Goldfine; Fiscal Year: 2006
    ..abstract_text> ..
  49. TRPM2 HTS Screening Assay Development(RMI)
    Andrew Scharenberg; Fiscal Year: 2005
    ..Using the 96 well plate format, we will also evaluate the overall method's properties and suitability for HTS using a library of compounds of known bioactivity. ..
  50. INTEGRATED EM-IMMUNOLABELING INSTRUMENTATION
    Kathryn Howell; Fiscal Year: 2001
    ..The group of scientist on this SGI has a history of collegial interactions, a background that argues well for the successful sharing of the instrumentation requested. ..
  51. Structural /Mechanistic Study of Lantibiotic Biosynthetic Enzymes & Autoimmunity
    Satish K Nair; Fiscal Year: 2010
    ..In this last aim, we will seek to utilize the principles of biosynthetic combinatorial chemistry to answer the question "Can novel lanthionine-bearing lantibiotics be designed from structure-based principles?" ..
  52. The role of reovirus factories in viral RNA regulation.
    Cathy Miller; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  53. DIETARY & GENETIC CONTROL OF ROS DAMAGE TO THE LENS
    Norman S Wolf; Fiscal Year: 2010
    ..These will aid in the search for initiating events and the mechanisms leading up to the cataract formation. ..
  54. ANALYSIS OF B-CELL RECEPTOR CALCIUM SIGNALING
    Andrew Scharenberg; Fiscal Year: 2004
    ..abstract_text> ..
  55. Structure and Folding of Frameshifting mRNA Pseudoknots
    DAVID GIEDROC; Fiscal Year: 2006
    ..These experiments will significantly enhance our understanding of how mRNA structure, stability and folding influence ribosomal recoding required for replication and propagation of infectious plant and human RNA viruses. ..
  56. X-RAY STUDIES OF SOD IN AMYOTROPHIC LATERAL SCLEROSIS
    PETER JOHN HART; Fiscal Year: 2010
    ....
  57. MECHANISM OF E. COLI TERMINATION FACTOR RHO
    Barbara Stitt; Fiscal Year: 2003
    ..The results will provide a better understanding of how the energy of ATP hydrolysis can be transduced to movement. ..
  58. MOLECULAR GENETICS OF PARAMECIUM
    Ching Kung; Fiscal Year: 2002
    ..Because a convenient transformation method with facilitate research of the whole community, we also plan to perfect methods of transforming paramecia en masse. ..
  59. HERPES SIMPLEX VIRUS ENTRY VIA ENDOCYTOSIS
    ANTHONY NICOLA; Fiscal Year: 2006
    ..Additionally, these studies should elucidate the mechanisms that target the incoming virus to the proper cellular compartments to initiate productive infection. [unreadable] [unreadable]..
  60. MECHANISMS OF PYRIDOXAL-5'-PHOSPHATE DEPENDENT ENZYMES
    Robert Phillips; Fiscal Year: 2004
    ..Thus the results of this research are likely to make a significant contribution to the goal of improving health care for Americans in the future. ..
  61. Structure/Function Studies on Flavoproteins
    Irina Sevrioukova; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  62. Remodeling of Telomeres by Telomere Associated Proteins
    SARAH COMPTON; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..