- Genome-wide analysis of the general stress response network in Escherichia coli: sigmaS-dependent genes, promoters, and sigma factor selectivity
Institut fur Biologie, Mikrobiologie, Freie Universitat Berlin, Königin Luise Str 12 16a, 14195 Berlin, Germany
J Bacteriol 187:1591-603. 2005
..Thus, not only the expression of genes within a regulatory network but also the architecture of the network itself can be subject to regulation...
- H++: a server for estimating pKas and adding missing hydrogens to macromolecules
John C Gordon
Department of Computer Science, Virginia Tech Blacksburg, VA 24061, USA
Nucleic Acids Res 33:W368-71. 2005
..The server is intended for a broad community of biochemists, molecular modelers, structural biologists and drug designers; it can also be used as an educational tool in biochemistry courses...
- Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese hamster ovary cells
Anthony V Nicola
Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
J Virol 77:5324-32. 2003
..The results suggest that HSV can utilize two distinct entry pathways, depending on the type of cell encountered...
- Impaired nociception and pain sensation in mice lacking the capsaicin receptor
M J Caterina
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94143 0450, USA
Science 288:306-13. 2000
..Thus, VR1 is essential for selective modalities of pain sensation and for tissue injury-induced thermal hyperalgesia...
- Whole-cell patch-clamp measurements of spermatozoa reveal an alkaline-activated Ca2+ channel
Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital and Harvard Medical School Enders 1309, Boston, Massachusetts 02115, USA
Nature 439:737-40. 2006
..Our results identify CatSper1 as a component of the key flagellar calcium channel, and suggest that intracellular alkalinization potentiates CatSper current to increase intraflagellar calcium and induce sperm hyperactivation...
- Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins
Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Nature 394:192-5. 1998
..When linked to a vesicle membrane protein, pHluorins were sorted to secretory and synaptic vesicles and reported transmission at individual synaptic boutons, as well as secretion and fusion pore 'flicker' of single secretory granules...
- The complete genome sequence of the gastric pathogen Helicobacter pylori
J F Tomb
Institute for Genomic Research, Rockville, Maryland 20850, USA
Nature 388:539-47. 1997
..pylori has a few regulatory networks, and a limited metabolic repertoire and biosynthetic capacity. Its survival in acid conditions depends, in part, on its ability to establish a positive inside-membrane potential in low pH...
- Herpes simplex virus type 1 enters human epidermal keratinocytes, but not neurons, via a pH-dependent endocytic pathway
Anthony V Nicola
Medical Virology Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 1888, USA
J Virol 79:7609-16. 2005
..Thus, HSV utilizes fundamentally different cellular entry pathways to infect important target cell populations...
- Prediction of "aggregation-prone" and "aggregation-susceptible" regions in proteins associated with neurodegenerative diseases
Amol P Pawar
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
J Mol Biol 350:379-92. 2005
- Amyloid formation under physiological conditions proceeds via a native-like folding intermediate
Thomas R Jahn
Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
Nat Struct Mol Biol 13:195-201. 2006
..The results demonstrate that aggregation pathways can involve self-assembly of highly native-like folding intermediates, and have implications for the prevention of this, and other, amyloid disorders...
- Structures and mechanisms of viral membrane fusion proteins: multiple variations on a common theme
Judith M White
Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908 0732, USA
Crit Rev Biochem Mol Biol 43:189-219. 2008
..Clearly, highly divergent proteins have converged on the same overall strategy to mediate fusion, an essential step in the life cycle of every enveloped virus...
- Heteromultimers of DEG/ENaC subunits form H+-gated channels in mouse sensory neurons
Christopher J Benson
Department of Internal Medicine and Psychiatry, and Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, IA 52242, USA
Proc Natl Acad Sci U S A 99:2338-43. 2002
..These data indicate that combinations of two or more DEG/ENaC subunits coassemble as heteromultimers to generate transient H(+)-gated currents in mouse DRG neurons...
- Isolation of a tarantula toxin specific for a class of proton-gated Na+ channels
Institut de Pharmacologie Moleculaire et Cellulaire, Centre National de la Recherche Scientifique, Sophia Antipolis, Valbonne, France
J Biol Chem 275:25116-21. 2000
..The new toxin is the first high affinity and highly selective pharmacological agent for this novel class of ionic channels. It will be important for future studies of their physiological and physio-pathological roles...
- Regulation and modulation of pH in the brain
Department of Physiology and Neuroscience, New York University School of Medicine, New York, NY 10016, USA
Physiol Rev 83:1183-221. 2003
..The functional significance of these compartmental, activity-dependent pH shifts is discussed...
- Combining conformational flexibility and continuum electrostatics for calculating pK(a)s in proteins
Roxana E Georgescu
Department of Physics, City College of New York, New York 10031, USA
Biophys J 83:1731-48. 2002
..Disallowing these motions changes the calculated pK(a)...
- Structures of vesicular stomatitis virus glycoprotein: membrane fusion revisited
CNRS, UMR2472, INRA, UMR1157, IFR 115, Virologie Moléculaire et Structurale, Gif sur Yvette, France
Cell Mol Life Sci 65:1716-28. 2008
..Besides the similarities with other viral fusion machineries, the fusion properties and structures of G also reveal some striking particularities that invite us to reconsider a few dogmas concerning fusion proteins...
- Alkaline pH homeostasis in bacteria: new insights
Alexander Silberman Institute of Life Sciences, Hebrew University, Jerusalem 91904, Israel
Biochim Biophys Acta 1717:67-88. 2005
..This review highlights the approaches, major findings and unresolved problems in alkaline pH homeostasis, focusing on the small number of well-characterized alkali-tolerant and extremely alkaliphilic bacteria...
- Crystal structure of the low-pH form of the vesicular stomatitis virus glycoprotein G
CNRS, Unité Mixte de Recherche UMR 2472, Institut Fédératif de Recherche IFR 115, Virologie Moléculaire et Structurale, 91198, Gif sur Yvette, France
Science 313:187-91. 2006
..The structure provides a framework for understanding the reversibility of the G conformational change. Unexpectedly, G is homologous to gB of herpesviruses, which raises important questions on viral evolution...
- Structure of dengue virus: implications for flavivirus organization, maturation, and fusion
Richard J Kuhn
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
Cell 108:717-25. 2002
..The structure suggests that flaviviruses, and by analogy also alphaviruses, employ a fusion mechanism in which the distal beta barrels of domain II of the glycoprotein E are inserted into the cellular membrane...
- Migration of human melanoma cells depends on extracellular pH and Na+/H+ exchange
Institute of Physiology II, University of Munster, Robert Koch Str 27b, D 48149 Munster, Germany
J Physiol 567:225-38. 2005
..We propose that pHe and NHE activity affect migration of human melanoma cells by modulating cell-matrix interactions. Migration is hindered when the interaction is too strong (acidic pHe) or too weak (alkaline pHe or NHE inhibition)...
- Comparative analysis of extreme acid survival in Salmonella typhimurium, Shigella flexneri, and Escherichia coli
Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile 36688, USA
J Bacteriol 177:4097-104. 1995
..The arginine AR system was only observed in E. coli and required stationary-phase induction in acidified complex medium. The product of the adi locus, arginine decarboxylase, was responsible for arginine-based acid survival...
- CFTR regulates phagosome acidification in macrophages and alters bactericidal activity
Dept of Neurobiology, Pharmacology and Physiology, University of Chicago, Chicago, IL 60637, USA
Nat Cell Biol 8:933-44. 2006
..We hypothesize that CFTR contributes to lysosomal acidification and that in its absence phagolysosomes acidify poorly, thus providing an environment conducive to bacterial replication...
- Expression of hypoxia-inducible cell-surface transmembrane carbonic anhydrases in human cancer
Science Applications International Corporation, Laboratory of Immunobiology, Bldg 560, NCI FCRDC, P O Box B, Frederick, MD 21702, USA
Am J Pathol 158:905-19. 2001
..Our studies show an important causal link between hypoxia, extracellular acidification, and induction or enhanced expression of these enzymes in human tumors...
- The mammalian sodium channel BNC1 is required for normal touch sensation
M P Price
Howard Hughes Medical Institute, Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242, USA
Nature 407:1007-11. 2000
..These data identify the BNC1 channel as essential for the normal detection of light touch and indicate that BNC1 may be a central component of a mechanosensory complex...
- Dps protects cells against multiple stresses during stationary phase
Molecular and Computational Biology Program, Department of Biological Sciences, University of Southern California, Los Angeles, California 90089 1340, USA
J Bacteriol 186:4192-8. 2004
..The protective roles of Dps are most likely achieved through a combination of functions associated with the protein-DNA binding and chromosome compaction, metal chelation, ferroxidase activity, and regulation of gene expression...
- Escherichia coli glutamate- and arginine-dependent acid resistance systems increase internal pH and reverse transmembrane potential
Department of Microbiology and Immunology, University of South Alabama College of Medicine, 307 University Blvd, Mobile, AL 36688, USA
J Bacteriol 186:6032-41. 2004
..Thus, reversing DeltaPsi may be a more important acid resistance strategy than maintaining a specific pHi value...
- Ligands for clathrin-mediated endocytosis are differentially sorted into distinct populations of early endosomes
Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA
Cell 124:997-1009. 2006
..This pre-early endosome sorting process begins at clathrin-coated vesicles, depends on microtubule-dependent motility, and appears to involve endocytic adaptors...
- Acid-sensing ion channel 2 (ASIC2) modulates ASIC1 H+-activated currents in hippocampal neurons
Candice C Askwith
Department of Internal Medicine, Howard Hughes Medical Institute, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
J Biol Chem 279:18296-305. 2004
..These findings indicate that the absolute and relative amounts of ASIC subunits determine the amplitude and properties of hippocampal H(+)-gated currents and therefore may contribute to normal physiology and pathophysiology...
- Ambient pH gene regulation in fungi: making connections
Miguel A Peñalva
Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Cientificas, Ramiro de Maeztu, Madrid, Spain
Trends Microbiol 16:291-300. 2008
..PacC/Rim101-mediated pH regulation is crucial to fungal pathogenicity...
- Intracellular acidosis enhances the excitability of working muscle
Thomas H Pedersen
Department of Physiology, University of Aarhus, DK 8000, Denmark
Science 305:1144-7. 2004
..These results implicate chloride ion channels in muscle function and emphasize that intracellular acidosis of muscle has protective effects during muscle fatigue...
- Glycoprotein D receptor-dependent, low-pH-independent endocytic entry of herpes simplex virus type 1
Richard S B Milne
Department of Microbiology, University of Pennsylvania, School of Dental Medicine, 215 Levy Building, 240 South 40th Street, Philadelphia, PA 19104 6002, USA
J Virol 79:6655-63. 2005
..Resistance to similar reagents should therefore not be taken as proof of HSV entry by a nonendosomal pathway. These data define a novel gD receptor-dependent acid-independent endocytic entry pathway for HSV...
- Hypoxia activates the capacity of tumor-associated carbonic anhydrase IX to acidify extracellular pH
Centre of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava, Slovak Republic
FEBS Lett 577:439-45. 2004
..Our findings suggest that hypoxia regulates both expression and activity of CA IX in order to enhance the extracellular acidification, which may have important implications for tumor progression...
- pH regulates genes for flagellar motility, catabolism, and oxidative stress in Escherichia coli K-12
Lisa M Maurer
Department of Biology, Higley Hall, 202 N College Dr, Kenyon College, Gambier, OH 43022, USA
J Bacteriol 187:304-19. 2005
- The acid-activated ion channel ASIC contributes to synaptic plasticity, learning, and memory
John A Wemmie
Department of Psychiatry, University of Iowa, Iowa City, IA 52242, USA
Neuron 34:463-77. 2002
..These results identify ASIC as a key component of acid-activated currents and implicate these currents in processes underlying synaptic plasticity, learning, and memory...
- Acid-sensing ion channel 3 matches the acid-gated current in cardiac ischemia-sensing neurons
S P Sutherland
The Vollum Institute, Oregon Health Sciences University, Portland, OR 97201 3098, USA
Proc Natl Acad Sci U S A 98:711-6. 2001
- Mutational evidence for an internal fusion peptide in flavivirus envelope protein E
S L Allison
Institute of Virology, University of Vienna, A 1095 Vienna, Austria
J Virol 75:4268-75. 2001
..These data support the hypothesis that the cd loop may be directly involved in interactions with target membranes during fusion...
- The effect of intravenous lactated Ringer's solution versus 0.9% sodium chloride solution on serum osmolality in human volunteers
E L Williams
Anesthesiology Department, Allegheny University Hospitals, Allegheny General Hospital, Pittsburgh, Pennsylvania 15212, USA
Anesth Analg 88:999-1003. 1999
..001). In healthy humans, an infusion of large volumes of LR, but not NS, transiently decreased serum osmolality, whereas acidosis associated with NS persisted and urinary output was slower with NS...
- Low concentrations of sodium dodecyl sulfate induce the extension of beta 2-microglobulin-related amyloid fibrils at a neutral pH
Division of Molecular Pathology, Department of Pathological Sciences, Faculty of Medical Sciences, University of Fukui, Fukui 910 1193, Japan
Biochemistry 43:11075-82. 2004
..g., phospholipids) that affect the conformation and stability of beta2-m and amyloid fibrils will have significant effects on the kinetics of Abeta2M fibril formation...
- pH-dependent regulation of lysosomal calcium in macrophages
Kenneth A Christensen
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
J Cell Sci 115:599-607. 2002
..Moreover, lysosomes could provide an intracellular source for physiological increases in cytosolic calcium levels...
- Impact of near-future ocean acidification on echinoderms
Department of Marine Ecology, The Sven Lovén Centre for Marine Sciences, University of Gothenburg, Kristineberg, Sweden
Ecotoxicology 19:449-62. 2010
..g. pH, temperature, toxicants) and organism health. However, available data allow us to conclude that near-future OA will have negative impact on echinoderm taxa with likely significant consequences at the ecosystem level...
- Functional implications of the localization and activity of acid-sensitive channels in rat peripheral nervous system
Diego Alvarez de la Rosa
Departments of Cellular and Molecular Physiology, and Neurology, Yale University School of Medicine, New Haven, CT 06520, USA
Proc Natl Acad Sci U S A 99:2326-31. 2002
..These functional properties and localization in DRG have profound implications for the putative functional roles of ASICs in the nervous system...
- Regulation of intracellular pH
Walter F Boron
Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520 8026, USA
Adv Physiol Educ 28:160-79. 2004
- Characterization of the monocarboxylate transporter 1 expressed in Xenopus laevis oocytes by changes in cytosolic pH
Physiologisch chemisches Institut der Universität, Hoppe Seyler Strasse 4, D 72076 Tubingen, Federal Republic of
Biochem J 333:167-74. 1998
..In addition to changes in the intracellular pH, lactate transport also induced deviations from the resting membrane potential...
- Conditions leading to high CO2 (>5 kPa) in waterlogged-flooded soils and possible effects on root growth and metabolism
School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley 6009, WA, Australia
Ann Bot 98:9-32. 2006
..Conditions conducive to development of high P(CO2) (5-70 kPa) during soil waterlogging and flooding are discussed. The scant information on responses of roots to high P(CO2) in terms of growth and metabolism is reviewed...
- Activation of lysosomal function during dendritic cell maturation
E Sergio Trombetta
Department of Cell Biology and Department of Immunobiology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, Post Office Box 208002, New Haven, CT 06520 8002, USA
Science 299:1400-3. 2003
..Lysosomal function in DCs thus appears to be specialized for the developmentally regulated processing of internalized antigens...
- Is bacterial vaginosis a stronger risk factor for preterm birth when it is diagnosed earlier in gestation?
Mark A Klebanoff
National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA
Am J Obstet Gynecol 192:470-7. 2005
..However, this contention is based on small numbers of women...
- Determinants of blood pH in health and disease
J A Kellum
University of Pittsburgh Medical Center, Division of Critical Care, Pittsburgh, Pennsylvania 15213 2582, USA
Crit Care 4:6-14. 2000
..All changes in blood pH, in health and in disease, occur through changes in these three variables. Clinical implications for these findings are also discussed...
- Proinflammatory cytokines, markers of cardiovascular risks, oxidative stress, and lipid peroxidation in patients with hyperglycemic crises
Frankie B Stentz
Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
Diabetes 53:2079-86. 2004
..Return of these values to normal levels with insulin therapy demonstrates a robust anti-inflammatory effect of insulin...
- In vitro gene delivery mediated by chitosan. effect of pH, serum, and molecular mass of chitosan on the transfection efficiency
Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan
Biomaterials 22:2075-80. 2001
..Heptamer (1.3 kDa) did not show any gene expression. Cationic liposome (lipofectin)-associated gene expression was inhibited by serum, while chitosan showed resistance to serum...
- Improved pKa calculations through flexibility based sampling of a water-dominated interaction scheme
Department of Biomolecular Sciences, UMIST, P O Box 88, Manchester M60 1QD, UK jim
Protein Sci 13:2793-805. 2004
- Preservative agents in foods. Mode of action and microbial resistance mechanisms
Unilever Research Division, Foods Laboratories, Vlaardingen, The Netherlands
Int J Food Microbiol 50:1-17. 1999
..Finally, in the concluding paragraphs, options for combination preservation systems are evaluated...
- In situ calibration and [H+] sensitivity of the fluorescent Na+ indicator SBFI
Department of Anatomy, University of British Columbia, 2177 Westbrook Mall, Vancouver, British Columbia, Canada V6T 1Z3
Am J Physiol Cell Physiol 280:C1623-33. 2001
..The magnitudes of the apparent changes in [Na+](i) varied with absolute [Na+](i), and a method was developed for correcting [Na+](i) values measured with SBFI for changes in intracellular pH...
- MRI of the tumor microenvironment
Robert J Gillies
Department of Biochemistry, Arizona Cancer Center, University of Arizona HSC, Tucson, Arizona 85724 5024, USA
J Magn Reson Imaging 16:430-50. 2002
..Examples are also discussed using electron MR, optical, and radionuclear imaging techniques...
- Vanilloid receptor-1 is essential for inflammatory thermal hyperalgesia
J B Davis
Department of Neuroscience Research, SmithKline Beecham Pharmaceuticals, Harlow, UK
Nature 405:183-7. 2000
..We conclude that VR1 is required for inflammatory sensitization to noxious thermal stimuli but also that alternative mechanisms are sufficient for normal sensation of noxious heat...
- Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure
Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, Wisconsin 53706, USA
Nat Struct Biol 9:431-5. 2002
..A wobble pair. Protonation of the C.A pair and metal binding are mutually antagonistic. These results support a ribozyme model for U6 RNA function and suggest a possible mechanism for the regulation of RNA splicing...
- Acid-evoked currents in cardiac sensory neurons: A possible mediator of myocardial ischemic sensation
C J Benson
Vollum Institute, Division of Cardiology, Oregon Health Sciences University, Portland, Ore 97201 3098, USA
Circ Res 84:921-8. 1999
..The extremely large amplitude and prevalent expression of acid-evoked current in CSNs imply a critical role for acidity in sensation associated with myocardial ischemia...
- Salmonella stress management and its relevance to behaviour during intestinal colonisation and infection
Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
FEMS Microbiol Rev 29:1021-40. 2005
..The role of quorum-sensing through luxS and sdiA is also considered as a regulator of virulence and colonisation...
- Polarization and polarizability assessed by protein amide acidity
Wadsworth Center, New York State Department of Health, School of Public Health, University at Albany SUNY, Empire State Plaza, Albany, New York 12201, USA
Biochemistry 48:6482-94. 2009
- Scleractinian coral species survive and recover from decalcification
Faculty of Life Sciences, Bar Ilan University, Ramat Gan, 52900, Israel
Science 315:1811. 2007
..This refugia, however, does not undermine the threats to reef ecosystems in a high carbon dioxide world...
- Effect of mild acid on gene expression in Staphylococcus aureus
Program in Molecular Pathogenesis, Skirball Institute, and Department of Microbiology, New York University School of Medicine, 540 First Ave, New York, NY 10016, USA
J Bacteriol 186:8407-23. 2004
..Key results are verified by gene fusion and Northern blot hybridization analyses. The results point to, but do not define, possible regulatory pathways by which the organism senses and responds to a pH stimulus...
- Immobilization of Candida rugosa lipase on chitosan with activation of the hydroxyl groups
Shao Hua Chiou
Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30043, Taiwan
Biomaterials 25:197-204. 2004
..The kinetic parameters Km and Vmax were determined for the free and immobilized lipase. The activation energy (Ea) was found to decrease for immobilization of lipase on chitosan beads...
- Nramp2 expression is associated with pH-dependent iron uptake across the apical membrane of human intestinal Caco-2 cells
School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, United Kingdom
J Biol Chem 275:1023-9. 2000
..Taken together, these data suggest that the expression of the Nramp2 transporter in human enterocytes may play an important role in intestinal iron absorption...
- A reversible pH-driven DNA nanoswitch array
Nanoscience Centre, University of Cambridge, 11 J J Thomson Avenue, Cambridge CB3 0FF, U K
J Am Chem Soc 128:2067-71. 2006
..5 nm and transduces this motion into an optical "on-and-off" nanoswitch...
- Nitric oxide block of outward-rectifying K+ channels indicates direct control by protein nitrosylation in guard cells
Laboratory of Plant Physiology and Biophysics, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom
Plant Physiol 136:4275-84. 2004
..Instead, our results strongly suggest that NO directly modifies the K+ channel or a closely associated regulatory protein, probably by nitrosylation of cysteine sulfhydryl groups...
- Crystal structure of glycoprotein B from herpes simplex virus 1
Ekaterina E Heldwein
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 320 Longwood Avenue, Boston, MA 02115, USA
Science 313:217-20. 2006
..The domain connectivity within a gB monomer would permit such a rearrangement, including long-range translocations linked to viral and cellular membranes...
- Functional characterization of homo- and heteromeric channel kinases TRPM6 and TRPM7
Center for Cardiology and Cardiovascular Biology, Department of Cell Biology, University of Connecticut Health Center, Farmington 06030, USA
J Gen Physiol 127:525-37. 2006
..The unique characteristics of these three channel types, TRPM6, TRPM7, and TRPM6/7, suggest that they may play different roles in vivo...
- Epithelial barrier function in vivo is sustained despite gaps in epithelial layers
Alastair J M Watson
Department of Medicine, University of Liverpool, Liverpool, United Kingdom
Gastroenterology 129:902-12. 2005
..The aim of this study was to use high-resolution in vivo light microscopy to investigate the mechanism of epithelial shedding and the site of the permeability barrier during cell shedding...
- Escherichia coli acid resistance: tales of an amateur acidophile
John W Foster
Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile, Alabama 36695, USA
Nat Rev Microbiol 2:898-907. 2004
..Surprisingly, certain microorganisms that grow at neutral pH have elegantly regulated systems that enable survival during excursions into acidic environments. The best-characterized acid-resistance system is found in E. coli...
- The catalytic properties of human carbonic anhydrase IX
Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, Florida 32610 0267, USA
Biochem Biophys Res Commun 288:666-9. 2001
..Human CA IX was very strongly inhibited by three classic sulfonamides and cyanate, with inhibition constants that are close to those for CA II...
- RIM101-dependent and-independent pathways govern pH responses in Candida albicans
Department of Microbiology, Columbia University, New York, New York 10032, USA
Mol Cell Biol 20:971-8. 2000
..Thus, pH governs gene expression and cellular differentiation in C. albicans through both RIM101-dependent and RIM101-independent pathways...
- pH and chemotherapy
University of Arizona Health Sciences Center, Cancer Center Division, Tucson 85724-5024, USA
Novartis Found Symp 240:199-211; discussion 265-8. 2001
..Our results suggest that agents such as sodium bicarbonate, Carbicarb and the diuretic furosemide--which are known to induce metabolic alkalosis in humans--may be useful in enhancing the efficacy of these treatment regimens in humans...
- Attenuated mutants of the intracellular bacterium Listeria monocytogenes obtained by single amino acid substitutions in listeriolysin O
Departement des Biotechnologies, Institut Pasteur, Paris, France
Mol Microbiol 4:2167-78. 1990
- Molecular cloning of a non-inactivating proton-gated Na+ channel specific for sensory neurons
Institut de Pharmacologie Moleculaire et Cellulaire, CNRS, 660 route des Lucioles, Sophia Antipolis, 06560 Valbonne, France
J Biol Chem 272:20975-8. 1997
- Identification of a diacylglycerol acyltransferase gene involved in accumulation of triacylglycerol in Mycobacterium tuberculosis under stress
Tatiana D Sirakova
Biomolecular Science Center, and Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, Orlando, 32816 2364, USA
Microbiology 152:2717-25. 2006
..If TG storage is needed for the long-term survival of M. tuberculosis under dormant conditions, the tgs1 product could be a suitable target for antilatency drugs...
- Hsp70 stabilizes lysosomes and reverts Niemann-Pick disease-associated lysosomal pathology
Apoptosis Department and Centre for Genotoxic Stress Research, Institute of Cancer Biology, Danish Cancer Society, DK 2100 Copenhagen, Denmark
Nature 463:549-53. 2010
..Taken together, these data open exciting possibilities for the development of new treatments for lysosomal storage disorders and cancer with compounds that enter the lysosomal lumen by the endocytic delivery pathway...
- In vitro conversion of full-length mammalian prion protein produces amyloid form with physical properties of PrP(Sc)
Olga V Bocharova
Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, MD 21201, USA
J Mol Biol 346:645-59. 2005
- Acidic extracellular pH induces vascular endothelial growth factor (VEGF) in human glioblastoma cells via ERK1/2 MAPK signaling pathway: mechanism of low pH-induced VEGF
Edwin L Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital, and Harvard Medical School, Boston, Massachusetts 02114, USA
J Biol Chem 277:11368-74. 2002
..These results show that acidic pH activates Ras and the ERK1/2 MAPK pathway to enhance VEGF transcription via AP-1, leading to increased VEGF production...
- Tumour hypoxia, chemotherapeutic resistance and hypoxia-related therapies
Aoife M Shannon
Department of Surgery, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, 9, Dublin, Ireland
Cancer Treat Rev 29:297-307. 2003
..Some innovative therapies are also detailed which may potentially minimise or eliminate these problems associated with targeting solid tumours...
- TRP-ML1 regulates lysosomal pH and acidic lysosomal lipid hydrolytic activity
Abigail A Soyombo
Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
J Biol Chem 281:7294-301. 2006
..Furthermore, dissipation of the acidic lysosomal pH of TRP-ML1(-/-) cells by nigericin or chloroquine reversed the lysosomal storage disease phenotype. These findings provide a new mechanism to account for the pathogenesis of MLIV...
- Structure of the LDL receptor extracellular domain at endosomal pH
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard Y4 206, Dallas, TX 75390, USA
Science 298:2353-8. 2002
..We propose a mechanism for lipoprotein release in the endosome whereby the beta propeller functions as an alternate substrate for the ligand-binding domain, binding in a calcium-dependent way and promoting lipoprotein release...
- The GAA*TTC triplet repeat expanded in Friedreich's ataxia impedes transcription elongation by T7 RNA polymerase in a length and supercoil dependent manner
Section on Genomic Structure and Function, Laboratory of Molecular and Cellular Biology, National Institute of Diabetes and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0830, USA
Nucleic Acids Res 28:2815-22. 2000
..The non-template (GAA) strand folds back creating a loop in the template strand, and the polymerase is paused at the distal triplex-duplex junction...
- Regulation of organelle acidity
Department of Physics, University of California, Berkeley, Berkeley, California 94720, USA
J Gen Physiol 117:329-44. 2001
- West Nile virus in complex with the Fab fragment of a neutralizing monoclonal antibody
Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907 2054, USA
Proc Natl Acad Sci U S A 103:12400-4. 2006
..The structure suggests that the E16 antibody neutralizes WNV by blocking the initial rearrangement of the E glycoprotein before fusion with a cellular membrane...
- Urease produced by Coccidioides posadasii contributes to the virulence of this respiratory pathogen
Department of Biology, Margaret Batts Tobin Building, Room 1 308E, University of Texas at San Antonio, 6900 North Loop 1604 West, San Antonio, TX 78249, USA
Infect Immun 74:504-15. 2006
..posadasii contribute to host tissue damage, which exacerbates the severity of coccidioidal infection and enhances the virulence of this human respiratory pathogen...
- Analysis of residues near the fusion peptide in the influenza hemagglutinin structure for roles in triggering membrane fusion
Department of Microbiology and Immunology, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
Virology 370:403-14. 2008
..3 relative to WT. The results are discussed in relation to possible mechanisms by which HA structural rearrangements are initiated at low pH and clade-specific differences near the fusion peptide...
- Effects of pH buffering on horizontal and ganglion cell light responses in primate retina: evidence for the proton hypothesis of surround formation
Christopher M Davenport
Neurobiology and Behavior Graduate Program, Department of Physiology and Biophysics, University of Washington, Seattle, Washington 98195, USA
J Neurosci 28:456-64. 2008
- Purification and characterization of pyranose oxidase from the white rot fungus Trametes multicolor
Division of Biochemical Engineering, Institute of Food Technology, University of Agricultural Sciences Vienna, A-1190 Vienna, Austria
Appl Environ Microbiol 67:3636-44. 2001
..An additional function could be reduction of quinones, key intermediates that are formed during mineralization of lignin...
- A glutamate-dependent acid resistance gene in Escherichia coli
B M Hersh
Department of Biology, Kenyon College, Gambier, Ohio 43022, USA
J Bacteriol 178:3978-81. 1996
..coli from cytoplasmic acidification. The role of the glutamate cycle is particularly important for cultures grown at neutral pH before exposure to extreme acid...
- Identification of specific histidines as pH sensors in flavivirus membrane fusion
Institute of Virology, Medical University of Vienna, 1095 Vienna, Austria
J Cell Biol 183:353-61. 2008
..Conserved histidines located outside this critical interface were found to be completely dispensable for triggering fusion...
- Class II virus membrane fusion proteins
Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
Virology 344:38-47. 2006
..Inhibition of the fusion protein refolding reaction confirms its importance in fusion and suggests new antiviral strategies for these medically important viruses...
- SLC4 base (HCO3 -, CO3 2-) transporters: classification, function, structure, genetic diseases, and knockout models
Division of Nephrology, David Geffen School of Medicine at UCLA, University of California Los Angeles, 10833 Le Conte Avenue, Rm 7 155 Factor Bldg, Los Angeles, CA 90095, USA
Am J Physiol Renal Physiol 290:F580-99. 2006
..This review focuses on recent advances in our understanding of the molecular organization and functional properties of SLC4 transporters and their role in disease...
- Nickel and extracellular acidification inhibit the water permeability of human aquaporin-3 in lung epithelial cells
Department of Woman and Child Health, Karolinska Institutet, S 171 76 Stockholm, Sweden
J Biol Chem 278:30037-43. 2003
..Our results imply that nickel and extracellular pH may modulate lung water clearance and that defective water clearance may be an early component of nickel-induced lung disease...
- Sea urchin fertilization in a warm, acidified and high pCO2 ocean across a range of sperm densities
Schools of Medical and Biological Sciences, University of Sydney, NSW 2006, Australia
Mar Environ Res 69:234-9. 2010
..It is important to identify where vulnerabilities lie across life histories and our results indicate that sea urchin fertilization is robust to climate change stressors. However, developmental stages may be vulnerable to ocean change...
- Microenvironment-induced cancer metastasis
E K Rofstad
Department of Biophysics, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo
Int J Radiat Biol 76:589-605. 2000
- pH regulation in anoxic plants
Hubert H Felle
Botanisches Institut I, Justus Liebig Universitat Giessen, Senckenbergstrasse 17, D 35390 Giessen, Germany
Ann Bot 96:519-32. 2005
- Alterations of intracellular pH homeostasis in apoptosis: origins and roles
INSERM UMR 620, Faculte des Sciences Pharmaceutiques et Biologiques, Universite Rennes I, 2 av Pr Léon Bernard, 35 043 Rennes cedex, France
Cell Death Differ 11:953-61. 2004
..Special attention is given to the possible role played by pHi-regulating transporters...
- Uniport of NH4+ by the root hair plasma membrane ammonium transporter LeAMT1;1
Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universitat Tubingen, Auf der Morgenstelle 1, Tubingen, Germany
J Biol Chem 277:13548-55. 2002
..5 and pH 8.5. LeAMT1;1 is concluded to mediate potential-driven NH(4+) uptake and retrieval depending on root membrane potential and NH(4+) concentration gradient...
- Glucosylceramide synthase is an essential regulator of pathogenicity of Cryptococcus neoformans
Philipp C Rittershaus
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
J Clin Invest 116:1651-9. 2006
..neoformans through the cell cycle. This study establishes C. neoformans GlcCer as a key virulence factor of cryptococcal pathogenicity, with important implications for future development of new antifungal strategies...
- Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) infection of human fibroblast cells occurs through endocytosis
Shaw M Akula
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
J Virol 77:7978-90. 2003
..Taken together, these findings suggest that for its infectious entry into HFF, HHV-8 uses clathrin-mediated endocytosis and a low-pH intracellular environment...
- Exocytosed protons feedback to suppress the Ca2+ current in mammalian cone photoreceptors
S H DeVries
Department of Ophthalmology and Visual Science, Houston Health Science Center, University of Texas, Houston, TX 77030, USA
Neuron 32:1107-17. 2001
..I now show that vesicular protons feed back to block the nearby calcium channels that mediate release. This local proton-mediated feedback is a novel mechanism through which neurons may regulate the release of transmitter...
- Amyloid fibril formation and disaggregation of fragment 1-29 of apomyoglobin: insights into the effect of pH on protein fibrillogenesis
CRIBI Biotechnology Centre, University of Padua, Viale G Colombo 3, 35121 Padua, Italy
J Mol Biol 367:1237-45. 2007
..The results observed here for apoMb(1-29) provide an experimental basis for explaining the effect of charge and pH on amyloid fibril formation by both unfolded and folded protein systems...