hydrogen ion concentration

Summary

Top Publications

  1. pmc Genome-wide analysis of the general stress response network in Escherichia coli: sigmaS-dependent genes, promoters, and sigma factor selectivity
    Harald Weber
    Institut fur Biologie, Mikrobiologie, Freie Universitat Berlin, Königin Luise Str 12 16a, 14195 Berlin, Germany
    J Bacteriol 187:1591-603. 2005
  2. pmc H++: a server for estimating pKas and adding missing hydrogens to macromolecules
    John C Gordon
    Department of Computer Science, Virginia Tech Blacksburg, VA 24061, USA
    Nucleic Acids Res 33:W368-71. 2005
  3. pmc Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese hamster ovary cells
    Anthony V Nicola
    Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:5324-32. 2003
  4. ncbi Impaired nociception and pain sensation in mice lacking the capsaicin receptor
    M J Caterina
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94143 0450, USA
    Science 288:306-13. 2000
  5. ncbi Whole-cell patch-clamp measurements of spermatozoa reveal an alkaline-activated Ca2+ channel
    Yuriy Kirichok
    Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital and Harvard Medical School Enders 1309, Boston, Massachusetts 02115, USA
    Nature 439:737-40. 2006
  6. ncbi Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins
    G Miesenbock
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 394:192-5. 1998
  7. ncbi The complete genome sequence of the gastric pathogen Helicobacter pylori
    J F Tomb
    Institute for Genomic Research, Rockville, Maryland 20850, USA
    Nature 388:539-47. 1997
  8. pmc Herpes simplex virus type 1 enters human epidermal keratinocytes, but not neurons, via a pH-dependent endocytic pathway
    Anthony V Nicola
    Medical Virology Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 1888, USA
    J Virol 79:7609-16. 2005
  9. ncbi Prediction of "aggregation-prone" and "aggregation-susceptible" regions in proteins associated with neurodegenerative diseases
    Amol P Pawar
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    J Mol Biol 350:379-92. 2005
  10. ncbi Amyloid formation under physiological conditions proceeds via a native-like folding intermediate
    Thomas R Jahn
    Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
    Nat Struct Mol Biol 13:195-201. 2006

Detail Information

Publications308 found, 100 shown here

  1. pmc Genome-wide analysis of the general stress response network in Escherichia coli: sigmaS-dependent genes, promoters, and sigma factor selectivity
    Harald Weber
    Institut fur Biologie, Mikrobiologie, Freie Universitat Berlin, Königin Luise Str 12 16a, 14195 Berlin, Germany
    J Bacteriol 187:1591-603. 2005
    ..Thus, not only the expression of genes within a regulatory network but also the architecture of the network itself can be subject to regulation...
  2. pmc H++: a server for estimating pKas and adding missing hydrogens to macromolecules
    John C Gordon
    Department of Computer Science, Virginia Tech Blacksburg, VA 24061, USA
    Nucleic Acids Res 33:W368-71. 2005
    ..The server is intended for a broad community of biochemists, molecular modelers, structural biologists and drug designers; it can also be used as an educational tool in biochemistry courses...
  3. pmc Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese hamster ovary cells
    Anthony V Nicola
    Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:5324-32. 2003
    ..The results suggest that HSV can utilize two distinct entry pathways, depending on the type of cell encountered...
  4. ncbi Impaired nociception and pain sensation in mice lacking the capsaicin receptor
    M J Caterina
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94143 0450, USA
    Science 288:306-13. 2000
    ..Thus, VR1 is essential for selective modalities of pain sensation and for tissue injury-induced thermal hyperalgesia...
  5. ncbi Whole-cell patch-clamp measurements of spermatozoa reveal an alkaline-activated Ca2+ channel
    Yuriy Kirichok
    Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital and Harvard Medical School Enders 1309, Boston, Massachusetts 02115, USA
    Nature 439:737-40. 2006
    ..Our results identify CatSper1 as a component of the key flagellar calcium channel, and suggest that intracellular alkalinization potentiates CatSper current to increase intraflagellar calcium and induce sperm hyperactivation...
  6. ncbi Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins
    G Miesenbock
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 394:192-5. 1998
    ..When linked to a vesicle membrane protein, pHluorins were sorted to secretory and synaptic vesicles and reported transmission at individual synaptic boutons, as well as secretion and fusion pore 'flicker' of single secretory granules...
  7. ncbi The complete genome sequence of the gastric pathogen Helicobacter pylori
    J F Tomb
    Institute for Genomic Research, Rockville, Maryland 20850, USA
    Nature 388:539-47. 1997
    ..pylori has a few regulatory networks, and a limited metabolic repertoire and biosynthetic capacity. Its survival in acid conditions depends, in part, on its ability to establish a positive inside-membrane potential in low pH...
  8. pmc Herpes simplex virus type 1 enters human epidermal keratinocytes, but not neurons, via a pH-dependent endocytic pathway
    Anthony V Nicola
    Medical Virology Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 1888, USA
    J Virol 79:7609-16. 2005
    ..Thus, HSV utilizes fundamentally different cellular entry pathways to infect important target cell populations...
  9. ncbi Prediction of "aggregation-prone" and "aggregation-susceptible" regions in proteins associated with neurodegenerative diseases
    Amol P Pawar
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    J Mol Biol 350:379-92. 2005
    ....
  10. ncbi Amyloid formation under physiological conditions proceeds via a native-like folding intermediate
    Thomas R Jahn
    Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
    Nat Struct Mol Biol 13:195-201. 2006
    ..The results demonstrate that aggregation pathways can involve self-assembly of highly native-like folding intermediates, and have implications for the prevention of this, and other, amyloid disorders...
  11. pmc Structures and mechanisms of viral membrane fusion proteins: multiple variations on a common theme
    Judith M White
    Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908 0732, USA
    Crit Rev Biochem Mol Biol 43:189-219. 2008
    ..Clearly, highly divergent proteins have converged on the same overall strategy to mediate fusion, an essential step in the life cycle of every enveloped virus...
  12. pmc Heteromultimers of DEG/ENaC subunits form H+-gated channels in mouse sensory neurons
    Christopher J Benson
    Department of Internal Medicine and Psychiatry, and Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, IA 52242, USA
    Proc Natl Acad Sci U S A 99:2338-43. 2002
    ..These data indicate that combinations of two or more DEG/ENaC subunits coassemble as heteromultimers to generate transient H(+)-gated currents in mouse DRG neurons...
  13. ncbi Isolation of a tarantula toxin specific for a class of proton-gated Na+ channels
    P Escoubas
    Institut de Pharmacologie Moleculaire et Cellulaire, Centre National de la Recherche Scientifique, Sophia Antipolis, Valbonne, France
    J Biol Chem 275:25116-21. 2000
    ..The new toxin is the first high affinity and highly selective pharmacological agent for this novel class of ionic channels. It will be important for future studies of their physiological and physio-pathological roles...
  14. ncbi Regulation and modulation of pH in the brain
    Mitchell Chesler
    Department of Physiology and Neuroscience, New York University School of Medicine, New York, NY 10016, USA
    Physiol Rev 83:1183-221. 2003
    ..The functional significance of these compartmental, activity-dependent pH shifts is discussed...
  15. pmc Combining conformational flexibility and continuum electrostatics for calculating pK(a)s in proteins
    Roxana E Georgescu
    Department of Physics, City College of New York, New York 10031, USA
    Biophys J 83:1731-48. 2002
    ..Disallowing these motions changes the calculated pK(a)...
  16. ncbi Structures of vesicular stomatitis virus glycoprotein: membrane fusion revisited
    S Roche
    CNRS, UMR2472, INRA, UMR1157, IFR 115, Virologie Moléculaire et Structurale, Gif sur Yvette, France
    Cell Mol Life Sci 65:1716-28. 2008
    ..Besides the similarities with other viral fusion machineries, the fusion properties and structures of G also reveal some striking particularities that invite us to reconsider a few dogmas concerning fusion proteins...
  17. pmc Alkaline pH homeostasis in bacteria: new insights
    Etana Padan
    Alexander Silberman Institute of Life Sciences, Hebrew University, Jerusalem 91904, Israel
    Biochim Biophys Acta 1717:67-88. 2005
    ..This review highlights the approaches, major findings and unresolved problems in alkaline pH homeostasis, focusing on the small number of well-characterized alkali-tolerant and extremely alkaliphilic bacteria...
  18. ncbi Crystal structure of the low-pH form of the vesicular stomatitis virus glycoprotein G
    Stephane Roche
    CNRS, Unité Mixte de Recherche UMR 2472, Institut Fédératif de Recherche IFR 115, Virologie Moléculaire et Structurale, 91198, Gif sur Yvette, France
    Science 313:187-91. 2006
    ..The structure provides a framework for understanding the reversibility of the G conformational change. Unexpectedly, G is homologous to gB of herpesviruses, which raises important questions on viral evolution...
  19. pmc Structure of dengue virus: implications for flavivirus organization, maturation, and fusion
    Richard J Kuhn
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Cell 108:717-25. 2002
    ..The structure suggests that flaviviruses, and by analogy also alphaviruses, employ a fusion mechanism in which the distal beta barrels of domain II of the glycoprotein E are inserted into the cellular membrane...
  20. pmc Migration of human melanoma cells depends on extracellular pH and Na+/H+ exchange
    Christian Stock
    Institute of Physiology II, University of Munster, Robert Koch Str 27b, D 48149 Munster, Germany
    J Physiol 567:225-38. 2005
    ..We propose that pHe and NHE activity affect migration of human melanoma cells by modulating cell-matrix interactions. Migration is hindered when the interaction is too strong (acidic pHe) or too weak (alkaline pHe or NHE inhibition)...
  21. pmc Comparative analysis of extreme acid survival in Salmonella typhimurium, Shigella flexneri, and Escherichia coli
    J Lin
    Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile 36688, USA
    J Bacteriol 177:4097-104. 1995
    ..The arginine AR system was only observed in E. coli and required stationary-phase induction in acidified complex medium. The product of the adi locus, arginine decarboxylase, was responsible for arginine-based acid survival...
  22. ncbi CFTR regulates phagosome acidification in macrophages and alters bactericidal activity
    Anke Di
    Dept of Neurobiology, Pharmacology and Physiology, University of Chicago, Chicago, IL 60637, USA
    Nat Cell Biol 8:933-44. 2006
    ..We hypothesize that CFTR contributes to lysosomal acidification and that in its absence phagolysosomes acidify poorly, thus providing an environment conducive to bacterial replication...
  23. pmc Expression of hypoxia-inducible cell-surface transmembrane carbonic anhydrases in human cancer
    S Ivanov
    Science Applications International Corporation, Laboratory of Immunobiology, Bldg 560, NCI FCRDC, P O Box B, Frederick, MD 21702, USA
    Am J Pathol 158:905-19. 2001
    ..Our studies show an important causal link between hypoxia, extracellular acidification, and induction or enhanced expression of these enzymes in human tumors...
  24. ncbi The mammalian sodium channel BNC1 is required for normal touch sensation
    M P Price
    Howard Hughes Medical Institute, Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242, USA
    Nature 407:1007-11. 2000
    ..These data identify the BNC1 channel as essential for the normal detection of light touch and indicate that BNC1 may be a central component of a mechanosensory complex...
  25. pmc Dps protects cells against multiple stresses during stationary phase
    Sudha Nair
    Molecular and Computational Biology Program, Department of Biological Sciences, University of Southern California, Los Angeles, California 90089 1340, USA
    J Bacteriol 186:4192-8. 2004
    ..The protective roles of Dps are most likely achieved through a combination of functions associated with the protein-DNA binding and chromosome compaction, metal chelation, ferroxidase activity, and regulation of gene expression...
  26. pmc Escherichia coli glutamate- and arginine-dependent acid resistance systems increase internal pH and reverse transmembrane potential
    Hope Richard
    Department of Microbiology and Immunology, University of South Alabama College of Medicine, 307 University Blvd, Mobile, AL 36688, USA
    J Bacteriol 186:6032-41. 2004
    ..Thus, reversing DeltaPsi may be a more important acid resistance strategy than maintaining a specific pHi value...
  27. pmc Ligands for clathrin-mediated endocytosis are differentially sorted into distinct populations of early endosomes
    Melike Lakadamyali
    Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA
    Cell 124:997-1009. 2006
    ..This pre-early endosome sorting process begins at clathrin-coated vesicles, depends on microtubule-dependent motility, and appears to involve endocytic adaptors...
  28. ncbi Acid-sensing ion channel 2 (ASIC2) modulates ASIC1 H+-activated currents in hippocampal neurons
    Candice C Askwith
    Department of Internal Medicine, Howard Hughes Medical Institute, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 279:18296-305. 2004
    ..These findings indicate that the absolute and relative amounts of ASIC subunits determine the amplitude and properties of hippocampal H(+)-gated currents and therefore may contribute to normal physiology and pathophysiology...
  29. ncbi Ambient pH gene regulation in fungi: making connections
    Miguel A Peñalva
    Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Cientificas, Ramiro de Maeztu, Madrid, Spain
    Trends Microbiol 16:291-300. 2008
    ..PacC/Rim101-mediated pH regulation is crucial to fungal pathogenicity...
  30. ncbi Intracellular acidosis enhances the excitability of working muscle
    Thomas H Pedersen
    Department of Physiology, University of Aarhus, DK 8000, Denmark
    Science 305:1144-7. 2004
    ..These results implicate chloride ion channels in muscle function and emphasize that intracellular acidosis of muscle has protective effects during muscle fatigue...
  31. pmc Glycoprotein D receptor-dependent, low-pH-independent endocytic entry of herpes simplex virus type 1
    Richard S B Milne
    Department of Microbiology, University of Pennsylvania, School of Dental Medicine, 215 Levy Building, 240 South 40th Street, Philadelphia, PA 19104 6002, USA
    J Virol 79:6655-63. 2005
    ..Resistance to similar reagents should therefore not be taken as proof of HSV entry by a nonendosomal pathway. These data define a novel gD receptor-dependent acid-independent endocytic entry pathway for HSV...
  32. ncbi Hypoxia activates the capacity of tumor-associated carbonic anhydrase IX to acidify extracellular pH
    Eliska Svastova
    Centre of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava, Slovak Republic
    FEBS Lett 577:439-45. 2004
    ..Our findings suggest that hypoxia regulates both expression and activity of CA IX in order to enhance the extracellular acidification, which may have important implications for tumor progression...
  33. pmc pH regulates genes for flagellar motility, catabolism, and oxidative stress in Escherichia coli K-12
    Lisa M Maurer
    Department of Biology, Higley Hall, 202 N College Dr, Kenyon College, Gambier, OH 43022, USA
    J Bacteriol 187:304-19. 2005
    ....
  34. ncbi The acid-activated ion channel ASIC contributes to synaptic plasticity, learning, and memory
    John A Wemmie
    Department of Psychiatry, University of Iowa, Iowa City, IA 52242, USA
    Neuron 34:463-77. 2002
    ..These results identify ASIC as a key component of acid-activated currents and implicate these currents in processes underlying synaptic plasticity, learning, and memory...
  35. pmc Acid-sensing ion channel 3 matches the acid-gated current in cardiac ischemia-sensing neurons
    S P Sutherland
    The Vollum Institute, Oregon Health Sciences University, Portland, OR 97201 3098, USA
    Proc Natl Acad Sci U S A 98:711-6. 2001
    ....
  36. pmc Mutational evidence for an internal fusion peptide in flavivirus envelope protein E
    S L Allison
    Institute of Virology, University of Vienna, A 1095 Vienna, Austria
    J Virol 75:4268-75. 2001
    ..These data support the hypothesis that the cd loop may be directly involved in interactions with target membranes during fusion...
  37. ncbi The effect of intravenous lactated Ringer's solution versus 0.9% sodium chloride solution on serum osmolality in human volunteers
    E L Williams
    Anesthesiology Department, Allegheny University Hospitals, Allegheny General Hospital, Pittsburgh, Pennsylvania 15212, USA
    Anesth Analg 88:999-1003. 1999
    ..001). In healthy humans, an infusion of large volumes of LR, but not NS, transiently decreased serum osmolality, whereas acidosis associated with NS persisted and urinary output was slower with NS...
  38. ncbi Low concentrations of sodium dodecyl sulfate induce the extension of beta 2-microglobulin-related amyloid fibrils at a neutral pH
    Suguru Yamamoto
    Division of Molecular Pathology, Department of Pathological Sciences, Faculty of Medical Sciences, University of Fukui, Fukui 910 1193, Japan
    Biochemistry 43:11075-82. 2004
    ..g., phospholipids) that affect the conformation and stability of beta2-m and amyloid fibrils will have significant effects on the kinetics of Abeta2M fibril formation...
  39. ncbi pH-dependent regulation of lysosomal calcium in macrophages
    Kenneth A Christensen
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
    J Cell Sci 115:599-607. 2002
    ..Moreover, lysosomes could provide an intracellular source for physiological increases in cytosolic calcium levels...
  40. ncbi Impact of near-future ocean acidification on echinoderms
    S Dupont
    Department of Marine Ecology, The Sven Lovén Centre for Marine Sciences, University of Gothenburg, Kristineberg, Sweden
    Ecotoxicology 19:449-62. 2010
    ..g. pH, temperature, toxicants) and organism health. However, available data allow us to conclude that near-future OA will have negative impact on echinoderm taxa with likely significant consequences at the ecosystem level...
  41. pmc Functional implications of the localization and activity of acid-sensitive channels in rat peripheral nervous system
    Diego Alvarez de la Rosa
    Departments of Cellular and Molecular Physiology, and Neurology, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 99:2326-31. 2002
    ..These functional properties and localization in DRG have profound implications for the putative functional roles of ASICs in the nervous system...
  42. ncbi Regulation of intracellular pH
    Walter F Boron
    Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520 8026, USA
    Adv Physiol Educ 28:160-79. 2004
    ....
  43. pmc Characterization of the monocarboxylate transporter 1 expressed in Xenopus laevis oocytes by changes in cytosolic pH
    S Broer
    Physiologisch chemisches Institut der Universität, Hoppe Seyler Strasse 4, D 72076 Tubingen, Federal Republic of
    Biochem J 333:167-74. 1998
    ..In addition to changes in the intracellular pH, lactate transport also induced deviations from the resting membrane potential...
  44. pmc Conditions leading to high CO2 (>5 kPa) in waterlogged-flooded soils and possible effects on root growth and metabolism
    Hank Greenway
    School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley 6009, WA, Australia
    Ann Bot 98:9-32. 2006
    ..Conditions conducive to development of high P(CO2) (5-70 kPa) during soil waterlogging and flooding are discussed. The scant information on responses of roots to high P(CO2) in terms of growth and metabolism is reviewed...
  45. ncbi Activation of lysosomal function during dendritic cell maturation
    E Sergio Trombetta
    Department of Cell Biology and Department of Immunobiology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, Post Office Box 208002, New Haven, CT 06520 8002, USA
    Science 299:1400-3. 2003
    ..Lysosomal function in DCs thus appears to be specialized for the developmentally regulated processing of internalized antigens...
  46. ncbi Is bacterial vaginosis a stronger risk factor for preterm birth when it is diagnosed earlier in gestation?
    Mark A Klebanoff
    National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA
    Am J Obstet Gynecol 192:470-7. 2005
    ..However, this contention is based on small numbers of women...
  47. pmc Determinants of blood pH in health and disease
    J A Kellum
    University of Pittsburgh Medical Center, Division of Critical Care, Pittsburgh, Pennsylvania 15213 2582, USA
    Crit Care 4:6-14. 2000
    ..All changes in blood pH, in health and in disease, occur through changes in these three variables. Clinical implications for these findings are also discussed...
  48. ncbi Proinflammatory cytokines, markers of cardiovascular risks, oxidative stress, and lipid peroxidation in patients with hyperglycemic crises
    Frankie B Stentz
    Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    Diabetes 53:2079-86. 2004
    ..Return of these values to normal levels with insulin therapy demonstrates a robust anti-inflammatory effect of insulin...
  49. ncbi In vitro gene delivery mediated by chitosan. effect of pH, serum, and molecular mass of chitosan on the transfection efficiency
    T Sato
    Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan
    Biomaterials 22:2075-80. 2001
    ..Heptamer (1.3 kDa) did not show any gene expression. Cationic liposome (lipofectin)-associated gene expression was inhibited by serum, while chitosan showed resistance to serum...
  50. pmc Improved pKa calculations through flexibility based sampling of a water-dominated interaction scheme
    Jim Warwicker
    Department of Biomolecular Sciences, UMIST, P O Box 88, Manchester M60 1QD, UK jim
    Protein Sci 13:2793-805. 2004
    ....
  51. ncbi Preservative agents in foods. Mode of action and microbial resistance mechanisms
    S Brul
    Unilever Research Division, Foods Laboratories, Vlaardingen, The Netherlands
    Int J Food Microbiol 50:1-17. 1999
    ..Finally, in the concluding paragraphs, options for combination preservation systems are evaluated...
  52. ncbi In situ calibration and [H+] sensitivity of the fluorescent Na+ indicator SBFI
    A Diarra
    Department of Anatomy, University of British Columbia, 2177 Westbrook Mall, Vancouver, British Columbia, Canada V6T 1Z3
    Am J Physiol Cell Physiol 280:C1623-33. 2001
    ..The magnitudes of the apparent changes in [Na+](i) varied with absolute [Na+](i), and a method was developed for correcting [Na+](i) values measured with SBFI for changes in intracellular pH...
  53. ncbi MRI of the tumor microenvironment
    Robert J Gillies
    Department of Biochemistry, Arizona Cancer Center, University of Arizona HSC, Tucson, Arizona 85724 5024, USA
    J Magn Reson Imaging 16:430-50. 2002
    ..Examples are also discussed using electron MR, optical, and radionuclear imaging techniques...
  54. ncbi Vanilloid receptor-1 is essential for inflammatory thermal hyperalgesia
    J B Davis
    Department of Neuroscience Research, SmithKline Beecham Pharmaceuticals, Harlow, UK
    Nature 405:183-7. 2000
    ..We conclude that VR1 is required for inflammatory sensitization to noxious thermal stimuli but also that alternative mechanisms are sufficient for normal sensation of noxious heat...
  55. ncbi Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure
    Anna Huppler
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Nat Struct Biol 9:431-5. 2002
    ..A wobble pair. Protonation of the C.A pair and metal binding are mutually antagonistic. These results support a ribozyme model for U6 RNA function and suggest a possible mechanism for the regulation of RNA splicing...
  56. ncbi Acid-evoked currents in cardiac sensory neurons: A possible mediator of myocardial ischemic sensation
    C J Benson
    Vollum Institute, Division of Cardiology, Oregon Health Sciences University, Portland, Ore 97201 3098, USA
    Circ Res 84:921-8. 1999
    ..The extremely large amplitude and prevalent expression of acid-evoked current in CSNs imply a critical role for acidity in sensation associated with myocardial ischemia...
  57. ncbi Salmonella stress management and its relevance to behaviour during intestinal colonisation and infection
    Ivan Rychlik
    Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
    FEMS Microbiol Rev 29:1021-40. 2005
    ..The role of quorum-sensing through luxS and sdiA is also considered as a regulator of virulence and colonisation...
  58. ncbi Polarization and polarizability assessed by protein amide acidity
    GRISELDA HERNANDEZ
    Wadsworth Center, New York State Department of Health, School of Public Health, University at Albany SUNY, Empire State Plaza, Albany, New York 12201, USA
    Biochemistry 48:6482-94. 2009
    ....
  59. ncbi Scleractinian coral species survive and recover from decalcification
    Maoz Fine
    Faculty of Life Sciences, Bar Ilan University, Ramat Gan, 52900, Israel
    Science 315:1811. 2007
    ..This refugia, however, does not undermine the threats to reef ecosystems in a high carbon dioxide world...
  60. pmc Effect of mild acid on gene expression in Staphylococcus aureus
    Brian Weinrick
    Program in Molecular Pathogenesis, Skirball Institute, and Department of Microbiology, New York University School of Medicine, 540 First Ave, New York, NY 10016, USA
    J Bacteriol 186:8407-23. 2004
    ..Key results are verified by gene fusion and Northern blot hybridization analyses. The results point to, but do not define, possible regulatory pathways by which the organism senses and responds to a pH stimulus...
  61. ncbi Immobilization of Candida rugosa lipase on chitosan with activation of the hydroxyl groups
    Shao Hua Chiou
    Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30043, Taiwan
    Biomaterials 25:197-204. 2004
    ..The kinetic parameters Km and Vmax were determined for the free and immobilized lipase. The activation energy (Ea) was found to decrease for immobilization of lipase on chitosan beads...
  62. ncbi Nramp2 expression is associated with pH-dependent iron uptake across the apical membrane of human intestinal Caco-2 cells
    S Tandy
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, United Kingdom
    J Biol Chem 275:1023-9. 2000
    ..Taken together, these data suggest that the expression of the Nramp2 transporter in human enterocytes may play an important role in intestinal iron absorption...
  63. ncbi A reversible pH-driven DNA nanoswitch array
    Dongsheng Liu
    Nanoscience Centre, University of Cambridge, 11 J J Thomson Avenue, Cambridge CB3 0FF, U K
    J Am Chem Soc 128:2067-71. 2006
    ..5 nm and transduces this motion into an optical "on-and-off" nanoswitch...
  64. pmc Nitric oxide block of outward-rectifying K+ channels indicates direct control by protein nitrosylation in guard cells
    Sergei Sokolovski
    Laboratory of Plant Physiology and Biophysics, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    Plant Physiol 136:4275-84. 2004
    ..Instead, our results strongly suggest that NO directly modifies the K+ channel or a closely associated regulatory protein, probably by nitrosylation of cysteine sulfhydryl groups...
  65. ncbi Crystal structure of glycoprotein B from herpes simplex virus 1
    Ekaterina E Heldwein
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 320 Longwood Avenue, Boston, MA 02115, USA
    Science 313:217-20. 2006
    ..The domain connectivity within a gB monomer would permit such a rearrangement, including long-range translocations linked to viral and cellular membranes...
  66. pmc Functional characterization of homo- and heteromeric channel kinases TRPM6 and TRPM7
    Mingjiang Li
    Center for Cardiology and Cardiovascular Biology, Department of Cell Biology, University of Connecticut Health Center, Farmington 06030, USA
    J Gen Physiol 127:525-37. 2006
    ..The unique characteristics of these three channel types, TRPM6, TRPM7, and TRPM6/7, suggest that they may play different roles in vivo...
  67. ncbi Epithelial barrier function in vivo is sustained despite gaps in epithelial layers
    Alastair J M Watson
    Department of Medicine, University of Liverpool, Liverpool, United Kingdom
    Gastroenterology 129:902-12. 2005
    ..The aim of this study was to use high-resolution in vivo light microscopy to investigate the mechanism of epithelial shedding and the site of the permeability barrier during cell shedding...
  68. ncbi Escherichia coli acid resistance: tales of an amateur acidophile
    John W Foster
    Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile, Alabama 36695, USA
    Nat Rev Microbiol 2:898-907. 2004
    ..Surprisingly, certain microorganisms that grow at neutral pH have elegantly regulated systems that enable survival during excursions into acidic environments. The best-characterized acid-resistance system is found in E. coli...
  69. ncbi The catalytic properties of human carbonic anhydrase IX
    T Wingo
    Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, Florida 32610 0267, USA
    Biochem Biophys Res Commun 288:666-9. 2001
    ..Human CA IX was very strongly inhibited by three classic sulfonamides and cyanate, with inhibition constants that are close to those for CA II...
  70. pmc RIM101-dependent and-independent pathways govern pH responses in Candida albicans
    D Davis
    Department of Microbiology, Columbia University, New York, New York 10032, USA
    Mol Cell Biol 20:971-8. 2000
    ..Thus, pH governs gene expression and cellular differentiation in C. albicans through both RIM101-dependent and RIM101-independent pathways...
  71. ncbi pH and chemotherapy
    N Raghunand
    University of Arizona Health Sciences Center, Cancer Center Division, Tucson 85724-5024, USA
    Novartis Found Symp 240:199-211; discussion 265-8. 2001
    ..Our results suggest that agents such as sodium bicarbonate, Carbicarb and the diuretic furosemide--which are known to induce metabolic alkalosis in humans--may be useful in enhancing the efficacy of these treatment regimens in humans...
  72. ncbi Attenuated mutants of the intracellular bacterium Listeria monocytogenes obtained by single amino acid substitutions in listeriolysin O
    E Michel
    Departement des Biotechnologies, Institut Pasteur, Paris, France
    Mol Microbiol 4:2167-78. 1990
    ....
  73. ncbi Molecular cloning of a non-inactivating proton-gated Na+ channel specific for sensory neurons
    R Waldmann
    Institut de Pharmacologie Moleculaire et Cellulaire, CNRS, 660 route des Lucioles, Sophia Antipolis, 06560 Valbonne, France
    J Biol Chem 272:20975-8. 1997
    ....
  74. pmc Identification of a diacylglycerol acyltransferase gene involved in accumulation of triacylglycerol in Mycobacterium tuberculosis under stress
    Tatiana D Sirakova
    Biomolecular Science Center, and Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, Orlando, 32816 2364, USA
    Microbiology 152:2717-25. 2006
    ..If TG storage is needed for the long-term survival of M. tuberculosis under dormant conditions, the tgs1 product could be a suitable target for antilatency drugs...
  75. ncbi Hsp70 stabilizes lysosomes and reverts Niemann-Pick disease-associated lysosomal pathology
    Thomas Kirkegaard
    Apoptosis Department and Centre for Genotoxic Stress Research, Institute of Cancer Biology, Danish Cancer Society, DK 2100 Copenhagen, Denmark
    Nature 463:549-53. 2010
    ..Taken together, these data open exciting possibilities for the development of new treatments for lysosomal storage disorders and cancer with compounds that enter the lysosomal lumen by the endocytic delivery pathway...
  76. ncbi In vitro conversion of full-length mammalian prion protein produces amyloid form with physical properties of PrP(Sc)
    Olga V Bocharova
    Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, MD 21201, USA
    J Mol Biol 346:645-59. 2005
    ....
  77. ncbi Acidic extracellular pH induces vascular endothelial growth factor (VEGF) in human glioblastoma cells via ERK1/2 MAPK signaling pathway: mechanism of low pH-induced VEGF
    Lei Xu
    Edwin L Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital, and Harvard Medical School, Boston, Massachusetts 02114, USA
    J Biol Chem 277:11368-74. 2002
    ..These results show that acidic pH activates Ras and the ERK1/2 MAPK pathway to enhance VEGF transcription via AP-1, leading to increased VEGF production...
  78. ncbi Tumour hypoxia, chemotherapeutic resistance and hypoxia-related therapies
    Aoife M Shannon
    Department of Surgery, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, 9, Dublin, Ireland
    Cancer Treat Rev 29:297-307. 2003
    ..Some innovative therapies are also detailed which may potentially minimise or eliminate these problems associated with targeting solid tumours...
  79. ncbi TRP-ML1 regulates lysosomal pH and acidic lysosomal lipid hydrolytic activity
    Abigail A Soyombo
    Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    J Biol Chem 281:7294-301. 2006
    ..Furthermore, dissipation of the acidic lysosomal pH of TRP-ML1(-/-) cells by nigericin or chloroquine reversed the lysosomal storage disease phenotype. These findings provide a new mechanism to account for the pathogenesis of MLIV...
  80. ncbi Structure of the LDL receptor extracellular domain at endosomal pH
    Gabby Rudenko
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard Y4 206, Dallas, TX 75390, USA
    Science 298:2353-8. 2002
    ..We propose a mechanism for lipoprotein release in the endosome whereby the beta propeller functions as an alternate substrate for the ligand-binding domain, binding in a calcium-dependent way and promoting lipoprotein release...
  81. pmc The GAA*TTC triplet repeat expanded in Friedreich's ataxia impedes transcription elongation by T7 RNA polymerase in a length and supercoil dependent manner
    E Grabczyk
    Section on Genomic Structure and Function, Laboratory of Molecular and Cellular Biology, National Institute of Diabetes and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0830, USA
    Nucleic Acids Res 28:2815-22. 2000
    ..The non-template (GAA) strand folds back creating a loop in the template strand, and the polymerase is paused at the distal triplex-duplex junction...
  82. pmc Regulation of organelle acidity
    M Grabe
    Department of Physics, University of California, Berkeley, Berkeley, California 94720, USA
    J Gen Physiol 117:329-44. 2001
    ....
  83. pmc West Nile virus in complex with the Fab fragment of a neutralizing monoclonal antibody
    Bärbel Kaufmann
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907 2054, USA
    Proc Natl Acad Sci U S A 103:12400-4. 2006
    ..The structure suggests that the E16 antibody neutralizes WNV by blocking the initial rearrangement of the E glycoprotein before fusion with a cellular membrane...
  84. pmc Urease produced by Coccidioides posadasii contributes to the virulence of this respiratory pathogen
    Fariba Mirbod-Donovan
    Department of Biology, Margaret Batts Tobin Building, Room 1 308E, University of Texas at San Antonio, 6900 North Loop 1604 West, San Antonio, TX 78249, USA
    Infect Immun 74:504-15. 2006
    ..posadasii contribute to host tissue damage, which exacerbates the severity of coccidioidal infection and enhances the virulence of this human respiratory pathogen...
  85. pmc Analysis of residues near the fusion peptide in the influenza hemagglutinin structure for roles in triggering membrane fusion
    Sudha Thoennes
    Department of Microbiology and Immunology, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    Virology 370:403-14. 2008
    ..3 relative to WT. The results are discussed in relation to possible mechanisms by which HA structural rearrangements are initiated at low pH and clade-specific differences near the fusion peptide...
  86. pmc Effects of pH buffering on horizontal and ganglion cell light responses in primate retina: evidence for the proton hypothesis of surround formation
    Christopher M Davenport
    Neurobiology and Behavior Graduate Program, Department of Physiology and Biophysics, University of Washington, Seattle, Washington 98195, USA
    J Neurosci 28:456-64. 2008
    ....
  87. pmc Purification and characterization of pyranose oxidase from the white rot fungus Trametes multicolor
    C Leitner
    Division of Biochemical Engineering, Institute of Food Technology, University of Agricultural Sciences Vienna, A-1190 Vienna, Austria
    Appl Environ Microbiol 67:3636-44. 2001
    ..An additional function could be reduction of quinones, key intermediates that are formed during mineralization of lignin...
  88. pmc A glutamate-dependent acid resistance gene in Escherichia coli
    B M Hersh
    Department of Biology, Kenyon College, Gambier, Ohio 43022, USA
    J Bacteriol 178:3978-81. 1996
    ..coli from cytoplasmic acidification. The role of the glutamate cycle is particularly important for cultures grown at neutral pH before exposure to extreme acid...
  89. pmc Identification of specific histidines as pH sensors in flavivirus membrane fusion
    Richard Fritz
    Institute of Virology, Medical University of Vienna, 1095 Vienna, Austria
    J Cell Biol 183:353-61. 2008
    ..Conserved histidines located outside this critical interface were found to be completely dispensable for triggering fusion...
  90. ncbi Class II virus membrane fusion proteins
    Margaret Kielian
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    Virology 344:38-47. 2006
    ..Inhibition of the fusion protein refolding reaction confirms its importance in fusion and suggests new antiviral strategies for these medically important viruses...
  91. ncbi SLC4 base (HCO3 -, CO3 2-) transporters: classification, function, structure, genetic diseases, and knockout models
    Alexander Pushkin
    Division of Nephrology, David Geffen School of Medicine at UCLA, University of California Los Angeles, 10833 Le Conte Avenue, Rm 7 155 Factor Bldg, Los Angeles, CA 90095, USA
    Am J Physiol Renal Physiol 290:F580-99. 2006
    ..This review focuses on recent advances in our understanding of the molecular organization and functional properties of SLC4 transporters and their role in disease...
  92. ncbi Nickel and extracellular acidification inhibit the water permeability of human aquaporin-3 in lung epithelial cells
    Marina Zelenina
    Department of Woman and Child Health, Karolinska Institutet, S 171 76 Stockholm, Sweden
    J Biol Chem 278:30037-43. 2003
    ..Our results imply that nickel and extracellular pH may modulate lung water clearance and that defective water clearance may be an early component of nickel-induced lung disease...
  93. ncbi Sea urchin fertilization in a warm, acidified and high pCO2 ocean across a range of sperm densities
    Maria Byrne
    Schools of Medical and Biological Sciences, University of Sydney, NSW 2006, Australia
    Mar Environ Res 69:234-9. 2010
    ..It is important to identify where vulnerabilities lie across life histories and our results indicate that sea urchin fertilization is robust to climate change stressors. However, developmental stages may be vulnerable to ocean change...
  94. ncbi Microenvironment-induced cancer metastasis
    E K Rofstad
    Department of Biophysics, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo
    Int J Radiat Biol 76:589-605. 2000
    ....
  95. pmc pH regulation in anoxic plants
    Hubert H Felle
    Botanisches Institut I, Justus Liebig Universitat Giessen, Senckenbergstrasse 17, D 35390 Giessen, Germany
    Ann Bot 96:519-32. 2005
    ....
  96. ncbi Alterations of intracellular pH homeostasis in apoptosis: origins and roles
    D Lagadic-Gossmann
    INSERM UMR 620, Faculte des Sciences Pharmaceutiques et Biologiques, Universite Rennes I, 2 av Pr Léon Bernard, 35 043 Rennes cedex, France
    Cell Death Differ 11:953-61. 2004
    ..Special attention is given to the possible role played by pHi-regulating transporters...
  97. ncbi Uniport of NH4+ by the root hair plasma membrane ammonium transporter LeAMT1;1
    Uwe Ludewig
    Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universitat Tubingen, Auf der Morgenstelle 1, Tubingen, Germany
    J Biol Chem 277:13548-55. 2002
    ..5 and pH 8.5. LeAMT1;1 is concluded to mediate potential-driven NH(4+) uptake and retrieval depending on root membrane potential and NH(4+) concentration gradient...
  98. pmc Glucosylceramide synthase is an essential regulator of pathogenicity of Cryptococcus neoformans
    Philipp C Rittershaus
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Clin Invest 116:1651-9. 2006
    ..neoformans through the cell cycle. This study establishes C. neoformans GlcCer as a key virulence factor of cryptococcal pathogenicity, with important implications for future development of new antifungal strategies...
  99. pmc Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) infection of human fibroblast cells occurs through endocytosis
    Shaw M Akula
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    J Virol 77:7978-90. 2003
    ..Taken together, these findings suggest that for its infectious entry into HFF, HHV-8 uses clathrin-mediated endocytosis and a low-pH intracellular environment...
  100. ncbi Exocytosed protons feedback to suppress the Ca2+ current in mammalian cone photoreceptors
    S H DeVries
    Department of Ophthalmology and Visual Science, Houston Health Science Center, University of Texas, Houston, TX 77030, USA
    Neuron 32:1107-17. 2001
    ..I now show that vesicular protons feed back to block the nearby calcium channels that mediate release. This local proton-mediated feedback is a novel mechanism through which neurons may regulate the release of transmitter...
  101. ncbi Amyloid fibril formation and disaggregation of fragment 1-29 of apomyoglobin: insights into the effect of pH on protein fibrillogenesis
    Paola Picotti
    CRIBI Biotechnology Centre, University of Padua, Viale G Colombo 3, 35121 Padua, Italy
    J Mol Biol 367:1237-45. 2007
    ..The results observed here for apoMb(1-29) provide an experimental basis for explaining the effect of charge and pH on amyloid fibril formation by both unfolded and folded protein systems...

Research Grants42

  1. Anatomic and Mechanical Variables of the EGJ in GERD
    John Pandolfino; Fiscal Year: 2006
    ..to improve standard pH monitoring technique by measuring acid exposure at the SCJ and converting data to hydrogen ion concentration exposure...
  2. EFFECTS OF HYPERTHERMIA ON IMMUNE FUNCTIONS IN VITRO
    EDDIE LAMON; Fiscal Year: 1992
    ..The effects of hydrogen ion concentration will be considered by monitoring pH and altering pH of the medium from 6.2 - 7.8...
  3. ACID AEROSOL HEALTH EFFECTS IN NORTH AMERICAN CHILDREN
    FRANK SPEIZER; Fiscal Year: 1991
    ..The monitoring methodology will provide separate measures of the contribution of sulfate and nitrate species to the total hydrogen ion concentration.
  4. CERAMIC WHISKER REINFORCEMENT OF DENTAL COMPOSITE RESINS
    HUAKUN XU; Fiscal Year: 2002
    ....
  5. F-18 FDG Peptides for Pretargeted PET Imaging of Pancrea
    William McBride; Fiscal Year: 2005
    ..It would also support the preclinical targeting, synthesis of new peptide derivatives to optimize excretion, serum stablity and safety studies in animals that would be needed before this agent goes into the clinic. ..
  6. Kinetics of Ligand Binding to Synaptic Ion Channels
    JAMES DILGER; Fiscal Year: 2006
    ..4. Use isothermal titration calorimetry to measure the thermodynamics of ligand binding to acetylcholine binding protein - a water soluble protein whose structure is being used as a template for the acetylcholine receptor. ..
  7. CONFORMATIONAL STABILITY OF GLOBULAR PROTEINS
    CARLOS PACE; Fiscal Year: 2006
    ..To gain a better understanding of protein solubility, we will study the effect of single changes in the amino acid sequence of RNase Sa on the solubility of the folded and unfolded states of the protein. ..
  8. Use of symbiotics to reduce urinary oxalate excretion
    JOHN LIESKE; Fiscal Year: 2007
    ..If results are positive, treatment for calcium oxalate kidney stones could be revolutionized. ..
  9. ALPHA-2 ADRENERGIC CONTROL OF COLONIC FUNCTION IN IBS
    Michael Camilleri; Fiscal Year: 2007
    ..abstract_text> ..
  10. C elegans:a model to study intestinal water-soluble vitamins transport in vivo
    HAMID SAID; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  11. Nephrolithiasis and Chronic Kidney Disease
    JOHN LIESKE; Fiscal Year: 2007
    ..This will gain additional urgency if in fact we demonstrate that NL is a significant risk factor for CKD. [unreadable] [unreadable] [unreadable]..
  12. Membrane Transport of NH3 and NH4+
    NAZIH NAKHOUL; Fiscal Year: 2008
    ..These are new properties of NH3/NH4+ transport that will help explain the role of NH3 and NH4+ in acidosis and their effect on transport of other ions a well. ..
  13. ALPHA 2 ADRENERGIC CONTROL IN IRRITABLE BOWEL SYNDROME
    Michael Camilleri; Fiscal Year: 2008
    ..Significance: This project will characterize pharmacogenetic mechanisms determining the response to clonidine through studies of candidate genes that control alpha2-adrenoreceptor function and norepinephrine transport in IBS. ..
  14. VOLTAGE-GATED PROTON CHANNELS IN HUMAN NEUTROPHILS
    THOMAS DECOURSEY; Fiscal Year: 2008
    ..5) We will identify the proton channel molecule and gene. ..
  15. Cannabinoid Mechanisms in Human Gastrointestinal Motor and Sensory Functions
    MICHAEL L CAMILLERI; Fiscal Year: 2010
    ..These actions may be relevant to develop new treatments for IBS. The studies will also provide further understanding of the potential of medications like medical marijuana on stomach function that may be relevant to appetite control. ..
  16. Tobacco Carcinogen Oral Cancer Model
    Joel Schwartz; Fiscal Year: 2005
    ..lc. Assessments of membrane (aryl hydrolase) and cytoplasmic phase I and II enzymes. ld. Confirmation of early cellular initiation and promotion events critical to our understanding for prevention of oral cancer. ..
  17. NOVEL INHIBITOR OF CRYSTAL ADHESION TO RENAL CELLS
    JOHN LIESKE; Fiscal Year: 2004
    ..Achieving these specific aims will provide new knowledge about mechanisms that mediate stone formation, and provide a rational basis for design of novel strategies to treat and/or prevent this disease. ..
  18. THE ROLE OF MYCOBACTERIA IN CROHN'S DISEASE
    David Graham; Fiscal Year: 2005
    ..The results of this study should either confirm or refute the proposed etiologic association of M. paratuberculosis and Crohn's disease as well as the identification of patients with Crohn's disease and M. paratuberculosis. ..
  19. Mechanism/regulation of human intestinal thiamin uptake
    HAMID SAID; Fiscal Year: 2008
    ..This should ultimately assist us in the designing of effective strategies to optimize thiamin body homeostasis in conditions associated with thiamin deficiency and sub-optimal levels. ..
  20. CHEMISTRY AND BIOLOGY OF INSULIN-LIKE VANADIUM COMPOUNDS
    DEBBIE CRANS; Fiscal Year: 2003
    ..The proposed studies will lead to an in-depth understanding of both the chemical and pharmacological properties of known and new vanadium compounds inducing insulin-like action. ..
  21. Molecular Basis of K+ Current During Secretion
    Keith Nehrke; Fiscal Year: 2002
    ..Finally, we will look at compensatory changes that occur in the expression of functionally-related ion transport proteins in the transgenic strain. ..
  22. A PF-GC for Environmental Health Breath Assessment
    Pavel Smejtek; Fiscal Year: 2004
    ..abstract_text> ..
  23. Na+/H+ exchange in the nematode intestine
    Keith Nehrke; Fiscal Year: 2004
    ..This multidisciplinary approach will help to define the biological role of individual Na+/H+ exchangers in the nematode intestine. ..
  24. A MICROSURGICAL INVESTIGATION OF GASTRIC ACID SECRETION
    John Geibel; Fiscal Year: 2004
    ..abstract_text> ..
  25. Role of Nanobacteria in Human Calcifying Disease
    JOHN LIESKE; Fiscal Year: 2004
    ..abstract_text> ..
  26. pH Regulation of Fat Storage in the Nematode Intestine
    Keith Nehrke; Fiscal Year: 2007
    ..The strength of this proposal is that it will use a powerful model system and novel reagents for monitoring pH in living organisms to study fat biology from a unique perspective: that of acid-base homeostasis. ..
  27. CARDIOTOXICITY OF STREPTOCOCCAL PYROGENIC EXOTOXINS
    Patrick Schlievert; Fiscal Year: 2006
    ....
  28. Oxalate-Induced Renal Scarring and Crystal Retention
    JOHN LIESKE; Fiscal Year: 2003
    ..Dr. Luis Juncos has extensive experience studying the regulation of renal blood flow in vivo, and will be critical in our efforts to develop the constant infusion model of hyperoxalemia and hyperoxaluria. ..
  29. INNOVATIVE BIOMONITORING FOR LEAD IN SALIVA
    Charles Timchalk; Fiscal Year: 2006
    ..It is reasonable to speculate that once this model system has been adequately validated it can readily be employed to screen sensitive populations (e.g. children) that are at greatest risk from chronic Pb exposure. ..
  30. Microbiology/Immunology of Periodontal Disease in T1DM
    Paul Moore; Fiscal Year: 2002
    ..An integrated multidisciplinary effort provides significant data collection capabilities essential for understanding the complex multifactorial etiology of periodontal disease in Type 1 diabetes. ..
  31. Development of Selective Nanoporous Sorbents for Radionuclide Decorporation
    Charles Timchalk; Fiscal Year: 2008
    ..abstract_text> ..
  32. Mixture Modeling: Pesticide Drug Interactions
    Charles Timchalk; Fiscal Year: 2009
    ..It is fully anticipated that this approach will facilitate the understanding of a variety of complex chemical interactions as it relates to the occupational health implications of working with insecticides. ..
  33. ORGANIZATION OF CENTRAL SYMPATHETIC PATHWAYS
    PATRICE GUYENET; Fiscal Year: 2008
    ..The research will contribute to the basic physiological knowledge needed to understand how syrr pathetic tone is regulated in health and in diseases such as arrhythmias, hypertension and dysautonomias. ..
  34. ORAL HEALTH OF OLDER TYPE 1 DIABETIC PATIENTS
    Paul Moore; Fiscal Year: 2003
    ..This overall plan will permit us to understand the etiology of these complications and to define specific subpopulations and risk factors appropriate for future prospective oral health prevention and intervention trials. ..
  35. CENTRAL NEURONAL MECHANISMS--DECOMPENSATED HEMORRHAGE
    PATRICE GUYENET; Fiscal Year: 2003
    ..Understanding the neurophysiological mechanisms triggered by hypotensive hemorrhage should lead to improved ability to manage this all too frequent clinical problem. ..
  36. H. Pylory factors associated with peptic ulcer disease
    TIMOTHY COVER; Fiscal Year: 2006
    ..pylori factors that help to determine clinical outcome, and ultimately, may lead to advances in the treatment or prevention of H. pylori-associated human diseases. [unreadable] [unreadable]..
  37. New Technology for Selective Delivery of PNAs in Cancer Cells In Vitro and In Viv
    OLEG ANDREEV; Fiscal Year: 2008
    ..The pHLIP nanosyringe could be a very effective tool for molecular analysis of cancer cells and diagnosis and treatment of cancer. [unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..
  38. HERPES SIMPLEX VIRUS ENTRY VIA ENDOCYTOSIS
    ANTHONY NICOLA; Fiscal Year: 2006
    ..Additionally, these studies should elucidate the mechanisms that target the incoming virus to the proper cellular compartments to initiate productive infection. [unreadable] [unreadable]..
  39. SIGNAL TRANSDUCERS IN SPERM
    HARVEY FLORMAN; Fiscal Year: 2004
    ..Consequently, these studies may also provide a model for the functions of TRP channels in other tissues. ..
  40. SPERM ION CHANNEL REGULATION
    HARVEY FLORMAN; Fiscal Year: 2002
    ..Moreover, an understanding of the regulation of this channel is a necessary step in attempts to design channel-based contraceptive agents. ..
  41. VOLTAGE-GATED PROTON CHANNELS IN ALVEOLAR EPITHELIUM
    THOMAS DECOURSEY; Fiscal Year: 2003
    ..Demonstration that H+ current fluctuations result from stochastic gating of H+ channels will provide strong evidence that the molecule is an ion channel rather than a carrier. ..