nitrosation

Summary

Summary: Conversion into nitroso compounds. An example is the reaction of nitrites with amino compounds to form carcinogenic N-nitrosamines.

Top Publications

  1. ncbi Extracellular S-nitrosoglutathione, but not S-nitrosocysteine or N(2)O(3), mediates protein S-nitrosation in rat spinal cord slices
    Jorge M Romero
    Department of Cell Biology and Physiology, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131, USA
    J Neurochem 99:1299-310. 2006
  2. pmc Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I
    Edward T Chouchani
    Medical Research Council Mitochondrial Biology Unit, Cambridge, UK
    Nat Med 19:753-9. 2013
  3. ncbi Nitrosative stress leads to protein glutathiolation, increased s-nitrosation, and up-regulation of peroxiredoxins in the heart
    Michael Reinartz
    Department of Cardiovascular Physiology, Heinrich Heine University Dusseldorf, Universitatsstrasse 1, Dusseldorf D 40225, Germany
    J Biol Chem 283:17440-9. 2008
  4. pmc Targeted disruption of inducible nitric oxide synthase protects against aging, S-nitrosation, and insulin resistance in muscle of male mice
    Eduardo R Ropelle
    Department of Internal Medicine, Faculty of Medical Sciences State University of Campinas, Campinas, Sao Paulo, Brazil
    Diabetes 62:466-70. 2013
  5. ncbi Protein denitrosylation: enzymatic mechanisms and cellular functions
    Moran Benhar
    Department of Medicine and Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nat Rev Mol Cell Biol 10:721-32. 2009
  6. pmc Effect of processed and red meat on endogenous nitrosation and DNA damage
    Annemiek M C P Joosen
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Carcinogenesis 30:1402-7. 2009
  7. pmc Chemical methods to detect S-nitrosation
    Hua Wang
    Department of Chemistry, Washington State University, Pullman, WA 99164, USA
    Curr Opin Chem Biol 15:32-7. 2011
  8. pmc Site-specific and redox-controlled S-nitrosation of thioredoxin
    Katherine T Barglow
    Department of Chemistry and QB3 Institute, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 108:E600-6. 2011
  9. pmc Nitric oxide in chemostat-cultured Escherichia coli is sensed by Fnr and other global regulators: unaltered methionine biosynthesis indicates lack of S nitrosation
    Steven T Pullan
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK
    J Bacteriol 189:1845-55. 2007
  10. pmc Protein S-nitrosylation in health and disease: a current perspective
    Matthew W Foster
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Trends Mol Med 15:391-404. 2009

Detail Information

Publications221 found, 100 shown here

  1. ncbi Extracellular S-nitrosoglutathione, but not S-nitrosocysteine or N(2)O(3), mediates protein S-nitrosation in rat spinal cord slices
    Jorge M Romero
    Department of Cell Biology and Physiology, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131, USA
    J Neurochem 99:1299-310. 2006
    ..The results clearly demonstrate that while all three NO-donors cause S-nitrosation of proteins in cell-free systems, only GSNO is a viable S-nitrosating agent in rat spinal cord slices...
  2. pmc Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I
    Edward T Chouchani
    Medical Research Council Mitochondrial Biology Unit, Cambridge, UK
    Nat Med 19:753-9. 2013
    ..Here we used a mitochondria-selective S-nitrosating agent, MitoSNO, to determine how mitochondrial S-nitrosation at the reperfusion phase of myocardial infarction is cardioprotective in vivo in mice...
  3. ncbi Nitrosative stress leads to protein glutathiolation, increased s-nitrosation, and up-regulation of peroxiredoxins in the heart
    Michael Reinartz
    Department of Cardiovascular Physiology, Heinrich Heine University Dusseldorf, Universitatsstrasse 1, Dusseldorf D 40225, Germany
    J Biol Chem 283:17440-9. 2008
    ..Another mechanism by which NO exerts biological effects is mediated through S-nitrosation of target proteins...
  4. pmc Targeted disruption of inducible nitric oxide synthase protects against aging, S-nitrosation, and insulin resistance in muscle of male mice
    Eduardo R Ropelle
    Department of Internal Medicine, Faculty of Medical Sciences State University of Campinas, Campinas, Sao Paulo, Brazil
    Diabetes 62:466-70. 2013
    Accumulating evidence has demonstrated that S-nitrosation of proteins plays a critical role in several human diseases...
  5. ncbi Protein denitrosylation: enzymatic mechanisms and cellular functions
    Moran Benhar
    Department of Medicine and Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nat Rev Mol Cell Biol 10:721-32. 2009
    ..In addition, they protect from nitrosative stress in microorganisms, mammals and plants, thereby exerting profound effects on host-microbe interactions and innate immunity...
  6. pmc Effect of processed and red meat on endogenous nitrosation and DNA damage
    Annemiek M C P Joosen
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Carcinogenesis 30:1402-7. 2009
    ..11 females), we investigated whether processing of meat might increase colorectal cancer risk by stimulating nitrosation and DNA damage. Meat diets contained 420 g (males) or 366 g (females) meat/per day...
  7. pmc Chemical methods to detect S-nitrosation
    Hua Wang
    Department of Chemistry, Washington State University, Pullman, WA 99164, USA
    Curr Opin Chem Biol 15:32-7. 2011
    ..Modification of cysteine residues by NO (or NO metabolites), that is S-nitrosation, changes the function of a broad spectrum of proteins...
  8. pmc Site-specific and redox-controlled S-nitrosation of thioredoxin
    Katherine T Barglow
    Department of Chemistry and QB3 Institute, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 108:E600-6. 2011
    Protein S-nitrosation on cysteine residues has emerged as an important posttranslational modification in mammalian cells. Previous studies have suggested a primary role for thioredoxin (Trx) in controlling protein S-nitrosation reactions...
  9. pmc Nitric oxide in chemostat-cultured Escherichia coli is sensed by Fnr and other global regulators: unaltered methionine biosynthesis indicates lack of S nitrosation
    Steven T Pullan
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK
    J Bacteriol 189:1845-55. 2007
    ..demonstrated the expression of nitric oxide (NO)-protective mechanisms, and obtained evidence of critical thiol nitrosation. The present study was the first to examine the transcriptome of NO-exposed E. coli in a chemostat...
  10. pmc Protein S-nitrosylation in health and disease: a current perspective
    Matthew W Foster
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Trends Mol Med 15:391-404. 2009
    ....
  11. pmc Comprehensive identification and modified-site mapping of S-nitrosylated targets in prostate epithelial cells
    Ying Wai Lam
    Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, Ohio, United States of America
    PLoS ONE 5:e9075. 2010
    ..Thus, our primary objective was to identify S-nitrosylated targets in an immortalized normal prostate epithelial cell line, NPrEC...
  12. ncbi Thioredoxin catalyzes the S-nitrosation of the caspase-3 active site cysteine
    Douglas A Mitchell
    Department of Chemistry, 211 Lewis Hall, University of California Berkeley, Berkeley, California 94720 1460, USA
    Nat Chem Biol 1:154-8. 2005
    ..Although key molecular details remain unanswered, S-nitrosation represents an example of cGMP-independent NO signaling...
  13. ncbi High glucose-induced tyrosine nitration in endothelial cells: role of eNOS uncoupling and aldose reductase activation
    Azza B el-Remessy
    Vascular Biology Center and Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, Georgia 30909, USA
    Invest Ophthalmol Vis Sci 44:3135-43. 2003
    ....
  14. ncbi Evaluation of oxidative and nitrosative stress in relapsing remitting multiple sclerosis: effect of corticosteroid therapy
    Aru Seven
    Department of Biochemistry, Istanbul University Medical Faculty, Istanbul, Turkey
    Folia Neuropathol 51:58-64. 2013
    ..As to nitrosative stress, nitrotyrosine and nitrite-nitrate levels were increased in CSF and decreased in serum...
  15. ncbi Nitric oxide inhibits caspase-3 by S-nitrosation in vivo
    L Rossig
    Molecular Cardiology, Department of Internal Medicine IV, University of Frankfurt, Theodor Stern Kai 7, 60590 Frankfurt, Germany
    J Biol Chem 274:6823-6. 1999
    ..In vitro data have demonstrated that NO inhibits the activity of caspase-3 by S-nitrosation of the enzyme...
  16. pmc Nitric oxide, S-nitrosation, and endothelial permeability
    Walter N Duran
    Department of Pharmacology and Physiology, New Jersey Medical School Rutgers, The State University of New Jersey, Newark, NJ, 07101 1709, U S A
    IUBMB Life 65:819-26. 2013
    S-Nitrosation is rapidly emerging as a regulatory mechanism in vascular biology, with particular importance in the onset of hyperpermeability induced by pro-inflammatory agents...
  17. ncbi Site-directed mutagenesis of aldehyde dehydrogenase-2 suggests three distinct pathways of nitroglycerin biotransformation
    M Verena Wenzl
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010, Austria
    Mol Pharmacol 80:258-66. 2011
    ..Although the latter reaction accounts for less than 10% of total turnover of GTN metabolism catalyzed by wild-type ALDH2, it is most likely essential for vascular GTN bioactivation...
  18. pmc S-nitrosylation of stargazin regulates surface expression of AMPA-glutamate neurotransmitter receptors
    Balakrishnan Selvakumar
    Solomon H Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 106:16440-5. 2009
    ..NMDAR stimulation, well known to activate neuronal nitric oxide synthase, increases both nitrosylation of stargazin and its binding to AMPAR. Thus, S-nitrosylation of stargazin is a physiologic regulator of AMPAR surface expression...
  19. ncbi Targeted disruption of iNOS prevents LPS-induced S-nitrosation of IRbeta/IRS-1 and Akt and insulin resistance in muscle of mice
    Marco A Carvalho-Filho
    Departamento de Clinica Medica, Universidade Estadual de Campinas, UNICAMP, 13081 970, Campinas SP, Brazil
    Am J Physiol Endocrinol Metab 291:E476-82. 2006
    ..iNOS) induction in two different models of obesity, diet-induced obesity and the ob/ob mice, is mediated by S-nitrosation of proteins involved in insulin signal transduction: insulin receptor beta-subunit (IRbeta), insulin receptor ..
  20. ncbi Inhibition of mitochondrial respiratory complex I by nitric oxide, peroxynitrite and S-nitrosothiols
    Guy C Brown
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK
    Biochim Biophys Acta 1658:44-9. 2004
    ..peroxynitrite and S-nitrosothiols that is reversible by light or reduced thiols, and therefore may be due to S-nitrosation or Fe-nitrosylation of the complex...
  21. ncbi Thioredoxin catalyzes the denitrosation of low-molecular mass and protein S-nitrosothiols
    Rajib Sengupta
    Department of Surgery, The University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Biochemistry 46:8472-83. 2007
    While most proteins have critical thiols whose oxidation affects their activity, it has been suggested that S-nitrosation and denitrosation of cellular thiols are fundamental processes similar to protein phosphorylation and ..
  22. ncbi S-nitrosation of Cys-800 of HIF-1alpha protein activates its interaction with p300 and stimulates its transcriptional activity
    Inna M Yasinska
    Department of Biochemistry, Mechnikov Odessa National University, Apt 208, Glushko Prospect 16, Odessa 65104, Ukraine
    FEBS Lett 549:105-9. 2003
    ..Recently HIF-1alpha, a subunit of HIF-1 complex, was characterized as a potential target for S-nitrosation, providing no information about the impact of this posttranslational modification on the protein ..
  23. ncbi Genotoxic and carcinogenic risk to humans of drug-nitrite interaction products
    Giovanni Brambilla
    Department of Internal Medicine, Division of Clinical Pharmacology and Toxicology, University of Genoa, Viale Benedetto XV, 2, I 16132 Genoa, Italy
    Mutat Res 635:17-52. 2007
    ..on the chemistry of NOC formation, the carcinogenic activity of NOC in animals and humans and the inhibitors of nitrosation reactions...
  24. ncbi S-nitrosation of the insulin receptor, insulin receptor substrate 1, and protein kinase B/Akt: a novel mechanism of insulin resistance
    Marco A Carvalho-Filho
    Department of Internal Medicine, State University of Campinas, UNICAMP, Campinas, Brazil
    Diabetes 54:959-67. 2005
    ..Here, we investigated whether this insulin resistance could be mediated by S-nitrosation of proteins involved in early steps of the insulin signal transduction pathway...
  25. ncbi Mechanisms of biological S-nitrosation and its measurement
    T Akaike
    Department of Microbiology, Kumamoto University School of Medicine, Japan
    Free Radic Res 33:461-9. 2000
    ..Understanding of the biologically relevant S-nitrosation mechanism is essential because RS-NOs seem to be critically involved in modulation of intracellular and ..
  26. pmc Activation of aminoimidazole carcinogens by nitrosation: mutagenicity and nucleotide adducts
    Terry V Zenser
    VA Medical Center and St Louis University, St Louis, MO, United States
    Mutat Res 673:109-15. 2009
    ..In summary, these N-nitrosamines are genotoxic and might be alternatives to their hydroxylamine analogues as activated intermediates leading to initiation of colon cancer in individuals with colitis...
  27. ncbi S-Nitrosation and regulation of inducible nitric oxide synthase
    Douglas A Mitchell
    Department of Chemistry, University of California, Berkeley, Berkeley, California 94720 1460, USA
    Biochemistry 44:4636-47. 2005
    ..7, and the biotin switch method were used to confirm endogenous S-nitrosation of iNOS...
  28. ncbi S-nitrosation of thioredoxin in the nitrogen monoxide/superoxide system activates apoptosis signal-regulating kinase 1
    Inna M Yasinska
    Department of Biochemistry, Mechnikov Odessa National University, Per Shampansky 2, Odessa 65058, Ukraine
    Arch Biochem Biophys 428:198-203. 2004
    In the present study, we have investigated S-nitrosation of reactive thioredoxin (Trx) thiol groups in nitric oxide/superoxide system...
  29. pmc S-nitrosation of arginase 1 requires direct interaction with inducible nitric oxide synthase
    Jessilyn Dunn
    Johns Hopkins University School of Medicine, 720 Rutland Ave Ross 1150, Baltimore, MD 21205, USA
    Mol Cell Biochem 355:83-9. 2011
    ..Here, we demonstrate that a direct interaction of Arg1 with NOS2 is necessary for its S-nitrosation. The specific domain of NOS2 that mediates this interaction is identified...
  30. ncbi Allosteric modulation by S-nitrosation in the low-O₂ affinity myoglobin from rainbow trout
    Signe Helbo
    Department of Biological Sciences, Aarhus University, Denmark
    Am J Physiol Regul Integr Comp Physiol 300:R101-8. 2011
    ..S-nitrosation at reactive cysteines may generate S-nitroso Mb (Mb-SNO) and contribute further to NO homeostasis...
  31. ncbi Protein S-nitrosation: biochemistry and characterization of protein thiol-NO interactions as cellular signals
    Shane Miersch
    Department of Chemistry and Biochemistry, University of Windsor, 401 Sunset Avenue, Windsor, Ontario, Canada N9B 3P4
    Clin Biochem 38:777-91. 2005
    ..transformed into species capable of adding an NO moiety to protein sulfhydryls, emphasizing how protein S-nitrosation differs from nitrosation of low molecular weight thiols...
  32. pmc Heme-assisted S-nitrosation desensitizes ferric soluble guanylate cyclase to nitric oxide
    Nathaniel B Fernhoff
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    J Biol Chem 287:43053-62. 2012
    ..of NO desensitization and find that sGC undergoes a reductive nitrosylation reaction that is coupled to the S-nitrosation of sGC cysteines...
  33. ncbi Development of an in vitro system combining aqueous and lipid phases as a tool to understand gastric nitrosation
    Emilie Combet
    Section of Human Nutrition, Faculty of Medicine, University of Glasgow, Yorkhill Hospital, Glasgow G3 8SF, UK
    Rapid Commun Mass Spectrom 24:529-34. 2010
    ..Ascorbic acid inhibits nitrosation by converting the nitrosative species into nitric oxide (NO)...
  34. ncbi Dietary N-nitroso compounds, endogenous nitrosation, and the risk of esophageal and gastric cancer subtypes in the Netherlands Cohort Study
    Andras P Keszei
    School for Oncology and Developmental Biology, Department of Epidemiology, Maastricht University, Maastricht, Netherlands
    Am J Clin Nutr 97:135-46. 2013
    Dietary N-nitroso compounds and endogenous nitrosation are important carcinogenic factors, but human evidence of their role is scarce for esophageal cancer and inconsistent for gastric cancer.
  35. ncbi SNObase, a database for S-nitrosation modification
    Xu Zhang
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Protein Cell 3:929-33. 2012
    ..Here we developed a database named SNObase ( http://www.nitrosation.org ), which collected S-nitrosation targets extracted from literatures up ..
  36. ncbi Microbial responses to nitric oxide and nitrosative stress: growth, "omic," and physiological methods
    Steven T Pullan
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom
    Methods Enzymol 437:499-519. 2008
    ..A key to the literature describing methods suitable for assessing toxicity to microbes of NO and reactive nitrogen species is given...
  37. ncbi Nitrosation of uric acid induced by nitric oxide under aerobic conditions
    Toshinori Suzuki
    School of Pharmacy, Shujitsu University, 1 6 1 Nishigawara, Okayama 703 8516, Japan
    Nitric Oxide 16:266-73. 2007
    ..These results indicate that under aerobic conditions nitric oxide can convert uric acid into its nitroso derivative, which can give a nitroso group to glutathione. Uric acid may act as a vehicle of nitric oxide in humans...
  38. ncbi Nitrosation, nitration, and autoxidation of the selective estrogen receptor modulator raloxifene by nitric oxide, peroxynitrite, and reactive nitrogen/oxygen species
    Violeta Toader
    Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 Wood Street, Chicago, Illinois 60612 7231, USA
    Chem Res Toxicol 16:1264-76. 2003
    ..decomposition of lower concentrations of SIN-1, was relatively unreactive toward E(2) and yielded oxidation and nitrosation products with raloxifene and its isomer...
  39. ncbi 2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potentiates nitrosation of a heterocyclic amine carcinogen by nitric oxide
    Vijaya M Lakshmi
    VA Medical Center, and Division of Geriatric Medicine, St Louis University School of Medicine, St Louis, Missouri 63125, USA
    Life Sci 80:644-9. 2007
    Although nitrosation plays an important role in initiation of carcinogenesis, the reactive nitrogen oxygen species (RNOS) mediating this reaction by multiple pathways have not been determined...
  40. ncbi Methodological vexation about thiol oxidation versus S-nitrosation -- a commentary on "An ascorbate-dependent artifact that interferes with the interpretation of the biotin-switch assay"
    Mark T Gladwin
    Vascular Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 41:557-61. 2006
  41. ncbi S-Nitrosation regulates the activation of endogenous procaspase-9 in HT-29 human colon carcinoma cells
    Ji Eun Kim
    Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 279:9758-64. 2004
    ..We show in this paper that cytokines and nitric-oxide synthase (NOS) inhibitors regulate S-nitrosation of an initiator caspase, procaspase-9, in a human colon adenocarcinoma cell line, HT-29...
  42. pmc Proteomic and mass spectroscopic quantitation of protein S-nitrosation differentiates NO-donors
    Vaishali Sinha
    Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois 60612 7231, USA
    ACS Chem Biol 5:667-80. 2010
    Protein S-nitrosation has been argued to be the most important signaling pathway mediating the bioactivity of NO. This post-translational modification of protein thiols is the result of chemical nitrosation of cysteine residues...
  43. pmc ESNOQ, proteomic quantification of endogenous S-nitrosation
    Xixi Zhou
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
    PLoS ONE 5:e10015. 2010
    S-nitrosation is a post-translational protein modification and is one of the most important mechanisms of NO signaling. Endogenous S-nitrosothiol (SNO) quantification is a challenge for detailed functional studies...
  44. ncbi Chemical considerations and biological selectivity of protein nitrosation: implications for NO-mediated signal transduction
    Benjamin Guikema
    Center for Cardiovascular Sciences, Albany Medical College, Albany, NY 12208, USA
    Antioxid Redox Signal 7:593-606. 2005
    ..body of literature indicating that many of the effects associated with the production of NO are due to the nitrosation of cysteine residues in proteins...
  45. ncbi Amino acid nitrosation products as alkylating agents
    M del P García-Santos
    Departamento de Quimica Fisica, Universidad de Salamanca, E 37008 Salamanca, Spain
    J Am Chem Soc 123:7506-10. 2001
    b>Nitrosation reactions of alpha-, beta-, and gamma-amino acids whose reaction products can act as alkylating agents of DNA were investigated...
  46. ncbi Detection of protein S-nitrosation using irreversible biotinylation procedures (IBP)
    Bo Huang
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China
    Free Radic Biol Med 49:447-56. 2010
    The biotin switch assay for detection of protein S-nitrosation has been widely used in the field of nitric oxide and redox signaling...
  47. pmc S-nitrosation: current concepts and new developments
    Douglas D Thomas
    Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, USA
    Antioxid Redox Signal 17:934-6. 2012
    The S-nitrosation (also referred to as S-nitrosylation) of cysteine residues is an important post-translational protein modification that regulates protein function and cell signaling...
  48. pmc Nitrosation, thiols, and hemoglobin: energetics and kinetics
    Willem H Koppenol
    Institute of Inorganic Chemistry, Department of Chemistry and Applied Biosciences, ETH Zurich, 8093 Zurich, Switzerland
    Inorg Chem 51:5637-41. 2012
    ..2) The electrode potential of the RSNO, H(+)/RSH, NO(•)(aq) couple is -0.20 ± 0.06 V at pH 7. (3) Thiol nitrosation by NO(2)(-) is favorable by 37 ± 5 kJ mol(-1) at pH 7...
  49. ncbi Nitric oxide and posttranslational modification of the vascular proteome: S-nitrosation of reactive thiols
    Diane E Handy
    Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Arterioscler Thromb Vasc Biol 26:1207-14. 2006
    ..S-nitrosation or thionitrite formation has only been found on a limited number of residues in a subset of proteins in in ..
  50. pmc A novel role for cytochrome c: Efficient catalysis of S-nitrosothiol formation
    Swati Basu
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Free Radic Biol Med 48:255-63. 2010
    ..Here, we demonstrate a novel mechanism by which cytochrome c may represent an efficient catalyst of S-nitrosation in vivo...
  51. ncbi Further study on S-nitrosation by nitrite
    Wu Nan Kuo
    Division of Science and Mathematics, Bethune Cookman College, Daytona Beach, Florida 32114, USA
    Front Biosci 8:a143-7. 2003
    ..Furthermore, the incubation of nitrite with IgG may generate the intermediates of active nitrogen/oxygen species and lead to significant production of gas bubbles...
  52. pmc Water-soluble triarylphosphines as biomarkers for protein S-nitrosation
    Erika Bechtold
    Department of Chemistry, Wake Forest University, Winston Salem, North Carolina 27109, USA
    ACS Chem Biol 5:405-14. 2010
    ..Several regulatory proteins are now verified targets of cellular S-nitrosation, and the direct detection of S-nitrosated residues in proteins has become essential to better understand RSNO-..
  53. ncbi Nitric oxide inhibits the cochaperone activity of the RING finger-like protein DnaJ
    K D Kröncke
    Research Group Immunobiology, Medical Department of the Heinrich Heine University of Düsseldorf, Moorenstrasse 5, Dusseldorf, D 40225, Germany
    Nitric Oxide 5:289-95. 2001
    ..This inhibition is accompanied by S-nitrosation of DnaJ as well as by Zn2+ release from the protein...
  54. ncbi Zn(II)-free dimethylargininase-1 (DDAH-1) is inhibited upon specific Cys-S-nitrosylation
    Markus Knipp
    Institute of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    J Biol Chem 278:3410-6. 2003
    ..Because Zn(II) protects the enzyme against nitrosation, it is suggested that both cysteines are involved in metal binding...
  55. ncbi Increased formation of methylglyoxal and protein glycation, oxidation and nitrosation in triosephosphate isomerase deficiency
    Naila Ahmed
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex CO4 3SQ, UK
    Biochim Biophys Acta 1639:121-32. 2003
    ..We investigated the changes in MG metabolism and markers of protein glycation, oxidation and nitrosation in a Hungarian family with two germline identical brothers, compound heterozygotes for triosephosphate ..
  56. ncbi Cell death: protein misfolding and neurodegenerative diseases
    Tomohiro Nakamura
    Center for Neuroscience, Aging and Stem Cell Research, Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    Apoptosis 14:455-68. 2009
    ..We discuss how memantine/NitroMemantine can inhibit excessive NMDA receptor activity to ameliorate NO production, protein misfolding, and neurodegeneration...
  57. ncbi New functions for the ancient globin family: bacterial responses to nitric oxide and nitrosative stress
    R K Poole
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    Mol Microbiol 36:775-83. 2000
    ....
  58. ncbi Inhibition of the transcription factor Yin Yang 1 activity by S-nitrosation
    Fumiya Hongo
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine and Jonsson Comprehensive Cancer Center at the University of California, Los Angeles, USA
    Biochem Biophys Res Commun 336:692-701. 2005
    ..167 (2001) 75-81]. YY1 is a zinc finger protein and thus, we hypothesized that NO inhibits YY1 activity via S-nitrosation of critical cysteines residues coordinated by Zn2+...
  59. pmc Nitric oxide controls nuclear export of APE1/Ref-1 through S-nitrosation of cysteines 93 and 310
    Jing Qu
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, and Graduate School of the Chinese Academy of Sciences, Beijing, China
    Nucleic Acids Res 35:2522-32. 2007
    ..This nuclear-cytoplasmic translocation was dependent on S-nitrosation modification of APE1, as simultaneous mutation of S-nitrosation target sites Cys93 and Cys310 completely ..
  60. pmc Cardioprotection and mitochondrial S-nitrosation: effects of S-nitroso-2-mercaptopropionyl glycine (SNO-MPG) in cardiac ischemia-reperfusion injury
    Sergiy M Nadtochiy
    Department of Anesthesiology, PO Box 604, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Mol Cell Cardiol 42:812-25. 2007
    ..Acknowledging the role of nitric oxide (NO()) in IPC, it was hypothesized that mitochondrial protein S-nitrosation may be a cardioprotective mechanism...
  61. ncbi S-nitrosation of glutathione by nitric oxide, peroxynitrite, and (*)NO/O(2)(*-)
    Astrid Schrammel
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Graz, Austria
    Free Radic Biol Med 34:1078-88. 2003
    To elucidate potential mechanisms of S-nitrosothiol formation in vivo, we studied nitrosation of GSH and albumin by nitric oxide ((*)NO), peroxynitrite, and (*)NO/O(2)(*)(-)...
  62. ncbi Role of S-nitrosation of cysteine residues in long-lasting inhibitory effect of nitric oxide on arterial tone
    Jacicarlos L Alencar
    Faculte de Pharmacie, Pharmacologie and Physico Chimie, Universite Louis Pasteur, Centre National de la Recherche Scientifique Unité Mixte Recherche 7034, 67401 Illkirch, France
    Mol Pharmacol 63:1148-58. 2003
    S-Nitrosation of cysteine residues plays an important role in nitric oxide (NO) signaling and transport...
  63. ncbi Effect of white versus red meat on endogenous N-nitrosation in the human colon and further evidence of a dose response
    Sheila Anne Bingham
    Medical Research Council, Dunn Human Nutrition Unit, Cambridge, UK CB2 2XY
    J Nutr 132:3522S-3525S. 2002
    ..To test the hypothesis that increased nitrogenous residues from red meat would increase endogenous N-nitrosation, thus accounting for the epidemiologic association between red meat consumption and colorectal cancer, we fed ..
  64. ncbi High glucose and oxidative/nitrosative stress conditions induce apoptosis in retinal endothelial cells by a caspase-independent pathway
    Ermelindo C Leal
    Center of Ophthalmology, IBILI, Faculty of Medicine, University of Coimbra, Portugal
    Exp Eye Res 88:983-91. 2009
    ..NOC-18 and H(2)O(2) also activate a caspase-independent apoptotic pathway, although H(2)O(2) can also induce caspase-mediated apoptosis...
  65. pmc CTA4 transcription factor mediates induction of nitrosative stress response in Candida albicans
    Wiriya Chiranand
    Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77251 1892, USA
    Eukaryot Cell 7:268-78. 2008
    ..albicans ssu1 mutants were not sensitive to NO, in contrast to findings in Saccharomyces cerevisiae. Cta4p is the first protein found to be necessary for initiating NO response in C. albicans...
  66. ncbi S-Nitrosoglutathione inactivation of the mitochondrial and cytosolic BCAT proteins: S-nitrosation and S-thiolation
    Steven J Coles
    Faculty of Health and Life Sciences, University of the West of England, Coldharbor Lane, Bristol BS16 1QY, UK
    Biochemistry 48:645-56. 2009
    ..with reactive thiol(ate) groups are susceptible to nitric oxide (NO) modification, which can result in S-nitrosation, S-thiolation, or disulfide bond formation...
  67. ncbi Inhibition of arterial contraction by dinitrosyl-iron complexes: critical role of the thiol ligand in determining rate of nitric oxide (NO) release and formation of releasable NO stores by S-nitrosation
    Jacicarlos L Alencar
    Faculte de Pharmacie, Pharmacologie and Physico Chimie, UMR CNRS 7034, Universite Louis Pasteur, 67401 Illkirch, France
    Biochem Pharmacol 66:2365-74. 2003
    ..Thus, the nature of the thiol ligand plays a critical role in determining the mechanisms and duration of the effect of LMW-DNIC in arteries...
  68. ncbi Processing of protein glycation, oxidation and nitrosation adducts in the liver and the effect of cirrhosis
    Naila Ahmed
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Central Campus, Colchester, Essex CO4 3SQ, UK
    J Hepatol 41:913-9. 2004
    Plasma proteins are modified non-enzymatically in vivo by glycation, oxidation and nitrosation processes...
  69. pmc Heme-assisted S-nitrosation of a proximal thiolate in a nitric oxide transport protein
    Andrzej Weichsel
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ 85721, USA
    Proc Natl Acad Sci U S A 102:594-9. 2005
    ....
  70. ncbi Inactivation of soluble guanylate cyclase by stoichiometric S-nitrosation
    Bernd Mayer
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Mol Pharmacol 75:886-91. 2009
    ..Based on the proposal that desensitization of sGC through S-nitrosation contributes to vascular NO resistance ( Proc Natl Acad Sci U S A 104: 12312-12317, 2007 ), we exposed purified ..
  71. ncbi Effect of vegetables, tea, and soy on endogenous N-nitrosation, fecal ammonia, and fecal water genotoxicity during a high red meat diet in humans
    Roisin Hughes
    Dunn Human Nutrition Unit, Medical Research Council, Cambridge CB2 2XY, UK
    Nutr Cancer 42:70-7. 2002
    Red meat increases colonic N-nitrosation, and this may explain the positive epidemiological relationship between red meat intake and colorectal cancer risk...
  72. ncbi Effect of dietary meat and fish on endogenous nitrosation, inflammation and genotoxicity of faecal water
    Annemiek M C P Joosen
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Mutagenesis 25:243-7. 2010
    ..We investigated the influence of isocaloric replacement of red meat with fatty fish on endogenous nitrosation, inflammation and genotoxicity of faecal water in apparently healthy human volunteers on controlled diets...
  73. ncbi [Drugs from the classes of tricyclic antidepressives and antiepileptics, nitrosatable under simulated human gastric conditions]
    D Ziebarth
    Zentralinstitut für Krebsforschung, Akademie der Wissenschaften der DDR, Robert Rössle Institut, Berlin Buch
    Arch Geschwulstforsch 59:257-63. 1989
    ..N-nitroso-dihydrodibenzazepine and N-nitroso-dibenzazepine could be identified by thin layer chromatography as main products. The biological effects of these N-nitroso compounds are not known up to now...
  74. ncbi Nitrotyrosine as a new marker for endogenous nitrosation and nitration of proteins
    H Ohshima
    International Agency for Research on Cancer, Unit of Environmental Carcinogens and Host Factors, Lyon, France
    Food Chem Toxicol 28:647-52. 1990
    ..8 +/- 2.3 (n = 11) were detected (detection limit 0.2 micrograms/litre). NTYR in proteins or its metabolites in urine can be readily analysed by GC-TEA as a new/additional marker for endogenous nitrosation and nitration.
  75. ncbi HIF-1 alpha protein as a target for S-nitrosation
    Vadim V Sumbayev
    University of Kaiserslautern, Faculty of Biology, Department of Cell Biology, Erwin Schrodinger Strasse, 67663 Kaiserslautern, Germany
    FEBS Lett 535:106-12. 2003
    ..that reactive nitrogen species are recognized for post-translation protein modifications, among others S-nitrosation, we asked whether HIF-1 alpha is a target for S-nitrosation...
  76. ncbi Dietary meat, endogenous nitrosation and colorectal cancer
    G G C Kuhnle
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, U K
    Biochem Soc Trans 35:1355-7. 2007
    ..Nitrosyl haem and other nitroso compounds can then form under the alkaline and reductive conditions of the small and large bowel...
  77. ncbi PI3 kinase and direct S-nitrosation are involved in down-regulation of apoptosis signal-regulating kinase 1 during LPS-induced Toll-like receptor 4 signalling
    Vadim V Sumbayev
    Medway School of Pharmacy, University of Kent, Anson Building, Central Avenue, Chatham Maritime, Kent ME4 4TB, United Kingdom
    Immunol Lett 115:126-30. 2008
    ..In addition, nitric oxide produced in response to exposure of THP-1 cells to LPS was found to S-nitrosate and therefore, down-regulate ASK1 activity...
  78. ncbi Regulation and specificity of S-nitrosylation and denitrosylation
    Steven R Tannenbaum
    Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    ACS Chem Biol 1:615-8. 2006
    ..A paper in this issue of the journal dissects the regulatory system involved in SNO of caspase-3 and highlights the need for more direct investigations of the complex biological regulation by NO signaling...
  79. pmc Balancing reactivity against selectivity: the evolution of protein S-nitrosylation as an effector of cell signaling by nitric oxide
    Behrad Derakhshan
    Weill Medical College of Cornell University, Department of Pharmacology, 1300 York Avenue, New York, NY 10021, USA
    Cardiovasc Res 75:210-9. 2007
    ....
  80. ncbi Alteration of S-nitrosothiol homeostasis and targets for protein S-nitrosation in human hepatocytes
    Laura M Lopez-Sanchez
    Liver Research Unit, Hospital Universitario Reina Sofia, Cordoba, Spain
    Proteomics 8:4709-20. 2008
    ..of cellular function and disease, and this study was aimed to identify potential targets for protein S-nitrosation during alteration of SNO homeostasis in human hepatocytes...
  81. pmc Direct evidence for S-nitrosation of mitochondrial complex I
    Lindsay S Burwell
    Department of Anesthesiology, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14620, USA
    Biochem J 394:627-34. 2006
    ..in mitochondrial complex IV, it has been proposed by several laboratories that complex I can be inhibited by S-nitrosation of a cysteine. However, direct molecular evidence for this is lacking...
  82. ncbi Pharmacological impairment of s-nitrosoglutathione or thioredoxin reductases augments protein S-Nitrosation in human hepatocarcinoma cells
    Laura M Lopez-Sanchez
    Unidad de Investigacion, Hospital Universitario Reina Sofia, Instituto Maimonides de Investigacion Biomedica de Cordoba, IMIBIC, 14004, Cordoba, Spain
    Anticancer Res 30:415-21. 2010
    ..Pharmacological interventions against these enzyme systems might represent valuable strategies to impair S-nitrosothiol (SNO) homeostasis in tumour cells...
  83. ncbi Persistent S-nitrosation of complex I and other mitochondrial membrane proteins by S-nitrosothiols but not nitric oxide or peroxynitrite: implications for the interaction of nitric oxide with mitochondria
    Christina C Dahm
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 281:10056-65. 2006
    S-nitrosation of mitochondrial proteins has been proposed to contribute to the pathophysiological interactions of nitric oxide (NO) and its derivatives with mitochondria but has not been shown directly...
  84. ncbi Nitric oxide cell signaling: S-nitrosation of Ras superfamily GTPases
    Kimberly W Raines
    Department of Biochemistry and Biophysics, University of North Carolina, 530 Mary Ellen Jones Building, Chapel Hill, NC 27599 7260, USA
    Cardiovasc Res 75:229-39. 2007
    ..This article reviews the mechanism of free radical-mediated GTPase regulation and shows nitric oxide can serve as important regulator of small GTPase proteins (i.e. Ras and RhoA) through protein modifications such as S-nitrosation.
  85. ncbi Signalling by NO-induced protein S-nitrosylation and S-glutathionylation: convergences and divergences
    Antonio Martínez-Ruiz
    Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Científicas and Instituto Reina Sofía de Investigaciones Nefrológicas, Ramiro de Maeztu, 9, Madrid E 28040, Spain
    Cardiovasc Res 75:220-8. 2007
    ....
  86. ncbi S-nitrosation and thiol switching in the mitochondrion: a new paradigm for cardioprotection in ischaemic preconditioning
    Bradford G Hill
    Department of Pathology, Molecular and Cellular Division, University of Alabama at Birmingham, Biomedical Research Building II, 901 19th Street South, Birmingham, AL 35294 2180, USA
    Biochem J 412:e11-3. 2008
    ..the Biochemical Journal in 2006, Burwell and colleagues addressed this issue and provided direct evidence for S-nitrosation of complex I of the mitochondrial electron transport chain...
  87. ncbi Protein cysteine thiol nitrosation: maker or marker of reactive nitrogen species-induced nonerythroid cellular signaling?
    Jack R Lancaster
    Center for Free Radical Biology, Department of Anesthesiology, University of Alabama Birmingham, Birmingham, AL, USA
    Nitric Oxide 19:68-72. 2008
    ..Much recent interest in the effects of ()NO in the setting of cancer has centered on the possible role of nitrosation (specifically, formation of nitrosothiol, RSNO) as a mechanism of protein-mediated signaling transduction...
  88. ncbi NO scavenging by 3-hydroxyanthranilic acid and 3-hydroxykynurenine: N-nitrosation leads via oxadiazoles to o-quinone diazides
    Claudia Backhaus
    Johann Friedrich Blumenbach Institute of Zoology and Anthropology, University of Goettingen, Berliner Strasse 28, D 37073 Goettingen, Lower Saxony, Germany
    Nitric Oxide 19:237-44. 2008
    ..b>Nitrosation of the non-hydroxylated analogs did not lead to colored products within the period of observation...
  89. ncbi Melatonin nitrosation promoted by NO*2; comparison with the peroxynitrite reaction
    Fabienne Peyrot
    Institut de Chimie des Substances Naturelles, CNRS, F 91198, Gif sur Yvette, France
    Free Radic Res 40:910-20. 2006
    ..Protein N-nitrosotryptophan is the best candidate for this N-nitroso pool. N-nitrosation of N-blocked trytophan derivatives like melatonin (MelH) by N2O3 or peroxynitrite (ONOOH/ONOO- ) has been ..
  90. ncbi Proteomic identification of S-nitrosylated proteins in endothelial cells
    Antonio Martínez-Ruiz
    Fundacion Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, Spain
    Methods Mol Biol 357:215-23. 2007
    ..by NO have been increasingly studied and, among them, cysteine modification by S-nitrosylation (also called S-nitrosation) has been hypothesized to represent a relevant mechanism for cell signaling...
  91. pmc Selective fluorescent labeling of S-nitrosothiols (S-FLOS): a novel method for studying S-nitrosation
    Lakshmi Santhanam
    The Johns Hopkins School of Medicine, Anesthesiology and CCM, 720 Rutland Avenue, Traylor 621, Baltimore, MD 21205, USA
    Nitric Oxide 19:295-302. 2008
    Protein S-nitrosation is a reversible post-translation modification critical for redox-sensitive cell signaling that is typically studied using the Biotin Switch method...
  92. ncbi Nitrosative stress in the ER: a new role for S-nitrosylation in neurodegenerative diseases
    Moran Benhar
    Department of Biochemistry, Duke University Medical Center, Box 2612, Durham, North Carolina, USA
    ACS Chem Biol 1:355-8. 2006
    ..Further, an examination of human brains afflicted with Parkinson's or Alzheimer's disease supports a causal role for the S-nitrosylation of PDI and consequent ER stress in these prevalent neurodegenerative disorders...
  93. ncbi Nitric oxide-induced inhibition of smooth muscle cell proliferation involves S-nitrosation and inactivation of RhoA
    Brian S Zuckerbraun
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Am J Physiol Cell Physiol 292:C824-31. 2007
    ..The purpose of these investigations was to examine the effect of NO on RhoA activation/S-nitrosation and to test the hypothesis that inhibition of SMC proliferation by NO is dependent on inactivation of RhoA...
  94. ncbi The role of a formaldehyde dehydrogenase-glutathione pathway in protein S-nitrosation in mammalian cells
    Arsalan S Haqqani
    Institute for Biological Sciences, National Research Council, Ottawa, Ont, Canada
    Nitric Oxide 9:172-81. 2003
    ..Intracellular sulfhydryls, both protein and non-protein, are potential targets of nitric oxide-related species. S-Nitrosation of proteins can occur in vivo and can affect their activity...
  95. ncbi Regiospecific nitrosation of N-terminal-blocked tryptophan derivatives by N2O3 at physiological pH
    Michael Kirsch
    Institut fur Physiologische Chemie, Universitatsklinikum, Hufelandstrasse 55, D 45122 Essen, Germany
    J Biol Chem 278:11931-6. 2003
    ..In the present study we examined the N-nitrosation of N-terminal-blocked (N-blocked) tryptophan derivatives in the presence of N(2)O(3) generating systems, such ..
  96. pmc Thioredoxin is required for S-nitrosation of procaspase-3 and the inhibition of apoptosis in Jurkat cells
    Douglas A Mitchell
    Department of Chemistry, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720 1460, USA
    Proc Natl Acad Sci U S A 104:11609-14. 2007
    S-nitrosation is a posttranslational, oxidative addition of NO to cysteine residues of proteins that has been proposed as a cGMP-independent signaling pathway [Hess DT, Matsumoto A, Kim SO, Marshall HE, Stamler JS (2005) Nat Rev Mol Cell ..
  97. ncbi Thiolation and nitrosation of cysteines in biological fluids and cells
    P Di Simplicio
    Department of Neuroscience, University of Siena, Italy
    Amino Acids 25:323-39. 2003
    ..When essential PSH are involved in S-thiolation and S-nitrosation reactions, a similar change in biological activity is observed...
  98. ncbi Nitration and nitrosation of N-acetyl-L-tryptophan and tryptophan residues in proteins by various reactive nitrogen species
    Toshinori Suzuki
    International Agency for Research on Cancer, 69372 Lyon Cedex 08, France
    Free Radic Biol Med 37:671-81. 2004
    ..Levels of 6-nitro-L-tryptophan and 3-nitro-L-tyrosine were similar in the nitrated protein. 6-Nitro-L-tryptophan in proteins can be measured as an additional biomarker of protein nitration...
  99. ncbi Site-directed mutagenesis studies of human serum albumin define tryptophan at amino acid position 214 as the principal site for nitrosation
    Krishna Harohalli
    Department of Biochemistry and Biophysics, John A Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA
    J Biomed Sci 9:47-58. 2002
    ..acidified NO(2)(-) was in agreement with previous reports which indicated that Cys-34 is the primary target for nitrosation in BSA...
  100. ncbi Human Ogg1, a protein involved in the repair of 8-oxoguanine, is inhibited by nitric oxide
    M Jaiswal
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    Cancer Res 61:6388-93. 2001
    ..These data support the concept that NO-mediated inhibition of DNA contributes to the mutagenic environment of chronic inflammation...
  101. ncbi Compartmentalized nitrosation and nitration in mitochondria
    Pedram Ghafourifar
    Department of Pharmacology and Therapeutics, LSU Health Sciences Center, Shreveport, LA 71130, USA
    Antioxid Redox Signal 5:349-54. 2003
    ..The mitochondrial intermembrane space provides an environment that favors S-nitrosation, whereas nitration occurs largely within the matrix...

Research Grants62

  1. A Hybrid PARP Inhibitor and Redox Catalyst for Lung Transplantation
    GARRY JOHN SOUTHAN; Fiscal Year: 2012
    ..respect to: 1) tissue damage (histology score, levels of neutrophil infiltration, lipid peroxidation, protein nitrosation, vascular permeability, PARP activation, and apoptosis) and arterial oxygenation;and 2) bronchoalveolar lavage ..
  2. A Redox-active PARP Inhibitor for Lung Transplantation
    ANDREW LURIE SALZMAN; Fiscal Year: 2012
    ..Tissue damage is assessed by histology, levels of PMN infiltration, lipid peroxidation, NF-?B, protein nitrosation, PARP-1 activation, apoptosis, and oxygenation (PaO2)...
  3. Hybrid Katp Channel Opener and Redox Catalyst for Lung Transplantation
    Prakash Jagtap; Fiscal Year: 2013
    ..Tissue damage is assessed by examining histologic score, PMN infiltration, lipid peroxidation, protein nitrosation, PARP-1 activation, nuclear NF-kB, apoptosis, and oxygenation (PaO2)...
  4. PARP inhibitor and Redox Catalyst Conjugate for Traumatic Brain Injury
    Kanneganti Murthy; Fiscal Year: 2012
    ..of redox and inflammatory injury, as manifested by histology score, levels of lipid peroxidation, protein nitrosation, PARP activation, apoptosis, and concentrations of TNF-1, MIP- 11, nuclear NF-: ...
  5. Arsenic co-carcinogenesis with UVR: nitrosation and oxidation of target proteins
    Ke Jian Liu; Fiscal Year: 2013
    ..Additionally, these studies may lead to testable hypotheses regarding potential arsenic targets in cancer and other arsenic-associated diseases. ..
  6. Genetic toxicology of purine metabolism
    Peter C Dedon; Fiscal Year: 2010
    ..data and it expands the repertoire of mechanisms for nucleobase deamination in DNA, including hydrolysis, nitrosation and deaminases...
  7. New mechanism and regulation of intracellular heme delivery in mammals
    Dennis J Stuehr; Fiscal Year: 2013
    ..Our initial studies suggest that NO inhibits cellular heme insertion into iNOS by promoting a specific S-nitrosation of GAPDH, thereby altering GAPDH properties that are independent of its enzyme activity...
  8. Serotonin Therapy for Parkinson's Disease and Neurodegenerative Disorders
    William A Wolf; Fiscal Year: 2013
    ..Specifically, we will determine how the approaches used in Aim II affect nitric oxide synthase activity and nitrosation of ASyn...
  9. Mechanism of Metabolism of S-Nitrosothiols
    Neil Hogg; Fiscal Year: 2010
    ..a range of lmwtRSNO and NO concentrations to uncover the differential sensitivity of proteins and pathways to S-nitrosation and S-thiolation...
  10. Role of Aldose Reductase in Diabetic Complications
    Satish K Srivastava; Fiscal Year: 2013
    ..We have shown that nitrosation activates and glutathiolation inactivates AR...
  11. GSTP1 PROMOTER HYPERMETHYLATION IN HUMAN PROSTATE CANCER
    WILLIAM GEORGE NELSON; Fiscal Year: 2012
    ..Specific Aims are proposed: (1) a characterization of post-translational modifications of DNMTs, including S-nitrosation in response to nitric oxide exposure, which promote de novo DNA methylation, (2) an evaluation of genome-wide ..
  12. Radiation-induced Nitric Oxide &Cellular Radiosensitivity
    Ross B Mikkelsen; Fiscal Year: 2012
    ..IR at clinically relevant doses (<5 Gy), activates Ca2+ dependent NOS, resulting in the transient S-nitrosation of PTP active site Cys and inhibition of cellular PTPs...
  13. S-Nitrosation in Nitric Oxide Signaling
    William R Montfort; Fiscal Year: 2010
    Protein S-nitrosation occurs throughout biology and plays a regulatory role in all major diseases, including cardiovascular disease, diabetes, asthma and cancer...
  14. Redox Transduction of Nitric Oxide Signaling
    BRUCE ALAN FREEMAN; Fiscal Year: 2013
    ..and yields secondary oxides of nitrogen (NOx) that expand the range of molecular targets of NO via oxidation, nitrosation and nitration reactions...
  15. FORMATION AND METABOLISM OF NITROSAMINES IN PIGS
    Peter Magee; Fiscal Year: 1991
    ..The drugs will include aminopyrine, known to yield dimethylnitrosamine on nitrosation, disulfiram reported to yield diethylnitrosamine, piperazine, and cimetidine which can be nitrosated to yield a ..
  16. NITRIC-OXIDE SYNTHASE FORMS N-NO-PTERIN AND S-NO-CYS
    ELIZABETH GETZOFF; Fiscal Year: 2011
    ..Here we define two specific non-heme iNOS nitrosation sites discovered by combining UV-visible spectroscopy, chemiluminescence, mass spectrometry, and x-ray ..
  17. Chemical approaches for detecting S-nitrosothiols
    Ming Xian; Fiscal Year: 2013
    ..Modification of cysteine residues by nitric oxide, i.e. S- nitrosation, changes the function of a broad spectrum of proteins...
  18. ELECTRON PARAMAGNETIC RESONANCE SPECTROMETER
    Cecilia Giulivi; Fiscal Year: 2009
    ..of malaria parasites and the putative antiparasite role of nitrosylhemoglobin;c) role of the oxidation, S-nitrosation/S-glutathionylation in the ryanodine receptor type 1;d) nitric oxide as an important modulator of cell ..
  19. CARCINOGENESIS--NITROSAMINE FORMATION AND INHIBITION
    RICHARD LOEPPKY; Fiscal Year: 1999
    ..incidence by preventing exposure to carcinogenic N-nitroso compounds or electrophilic species produced in nitrosation reactions...
  20. Metabolism and Carcinogenicity of Heterocyclic Amines
    TERRY ZENSER; Fiscal Year: 2007
    ..RNOS catalyze nitrosation, oxidation, and nitration reactions...
  21. ROLE OF ETHANOL IN THE ETIOLOGY OF HEAD & NECK CANCER
    G McCoy; Fiscal Year: 1992
    ..reactive intermediates (iminium ion species) which have also been suggested as intermediates for the clinical nitrosation of nicotine, we plan to determine if in situ nitrosation of nicotine can be catalyzed by isolated microsomes...
  22. MODULATION OF EGF-RECEPTOR SIGNALING
    Albert van Der Vliet; Fiscal Year: 2001
    ..These reactive nitrogen species can induce specific modifications in proteins, such as nitration and nitrosation products, which may represent unique modulatory mechanisms of protein and enzyme function...
  23. NITRIC OXIDE SIGNALING IN ALLERGIC AIRWAY DISEASE
    Albert van Der Vliet; Fiscal Year: 2007
    ..cell studies have illustrated inhibitory effects of NO" on NF ?B mediated gene expression, presumably by S-nitrosation of critical proteins...
  24. CHEMISTRY AND BIOCHEMISTRY OF NO RELEASING AGENTS
    Peng Wang; Fiscal Year: 2001
    ..our laboratory that N- nitrosomine compounds such as substituted N-methyl-N-nitrosoanilines carry out protein S-nitrosation (NBO+ transfer reaction) in aqueous solution at neutral pH. The research program include two specific aims; 1...
  25. NONVOLATILE N-NITROSO COMPOUNDS IN FOODS/GASTRIC JUICE
    SIDNEY MIRVISH; Fiscal Year: 1999
    ..4. Since creatinine and 2 oximes derived from it by nitrosation are the most likely food constituents to yield nitrosoureas, these 3 compounds will be nitrosated and the ..
  26. INTESTINAL METAPLASIA, ASCORBATE, AND H PYLORI
    Julie Parsonnet; Fiscal Year: 1993
    ..by increased cell proliferation, increased production of inflammation-related free radicals, and increased N-nitrosation of nitrites. We further hypothesize that these latter two effects are in part attributable to H...
  27. BACTERIA-DNA DAMAGE IN STOMACH AND BLADDER CANCERS
    Helmut Bartsch; Fiscal Year: 1991
    ..Unknown carcinogen spectrometry. Bacterial enzymes responsible for nitrosation of amine/amides will be characterized...
  28. NO, GUANYLATE CYCLASE AND IMMUNOTHERAPY OF CANCER
    Paul Shami; Fiscal Year: 2000
    Nitric oxide (NO) is cytotoxic to cancer cells by inducing protein tyrosine nitrosation protein thiol nitrosylation, protein ADP ribosylation, inhibiting mitochondrial respiration, and inhibiting ribonucleotide reductase...
  29. HEPATOCYTE:KUPFFER CELL INTERACTIONS IN SURGICAL SEPSIS
    Richard Simmons; Fiscal Year: 2003
    ..localization to peroxisomes favors the conversion of NO to reaction products that can carry out nitration and nitrosation. Under AIM 2, the molecular interactions between the caspase family of enzymes and iNOS will be defined...
  30. Specificity and Control of Signaling by S-Nitrosation
    Michael A Marletta; Fiscal Year: 2010
    The controls governing S-nitrosation are unknown. In addition, the ramifications of S-nitrosation signaling are virtually unknown...
  31. Accelerated NO/02 Reactions in Low Density Lipoprotein
    Jack Lancaster; Fiscal Year: 2007
    ..Specifically, a mechanistic rationale is proposed for examining a relative shift in nitrosation vs. nitration under biological conditions...
  32. IN-VIVO MICROCAPSULE MONITORING OF CARCINOGENS
    IAN O NEILL; Fiscal Year: 1993
    ..for mass spectral and HPLC identification of endogenous reactive electrophiles, ii) PEI as an efficient N-nitrosation substrate; B) to clarify significance of endogenous GI agents already found by 3 other microcapsule end-points ..
  33. REACTIONS BETWEEN THIOLS, NITRIC OXIDE AND PEROXYNITRITE
    Neil Hogg; Fiscal Year: 2001
    ..between thiols and S-nitrosothiols and the reduction of S-nitrosothiols to form the nitroxyl anion and (iii) S-nitrosation of cysteinyl residues of nitric oxide synthase (NOS) regulates NOS activity...
  34. PEROXYNITRITE AND NITROTYROSINE IN HEART DISEASE
    JOSEPH BECKMAN; Fiscal Year: 2002
    ..His first aim is to quantitatively assess the magnitude of tyrosine nitrosation in ischemic rat heart and to directly measure peroxynitrite using a novel probe they have developed...
  35. A nitric oxide(NO)-based metabonomic approach to investigate tobacco addiction
    Martin Feelisch; Fiscal Year: 2007
    ..globally affects the fate and biological actions of NO in the brain and other organs, leading to local nitrosation, nitration and oxidation of biomolecules with profound alterations in protein function and activity...
  36. Protein Damage in Oxidative Stress and Disease
    DANIEL LIEBLER; Fiscal Year: 2006
    ..describe the application of tandem mass spectrometry to characterize redox-sensitive protein thiol targets of S-nitrosation reactions involved in oxidative and nitrative stress and describe the analysis of protein targets relevant to ..
  37. Signaling of S-nitrosoalbumin in Pulmonary Endothelium
    Li Ming Zhang; Fiscal Year: 2009
    ..cell metabolism and permeability via NO release (and activation of soluble guanylyl cyclase) and/or S(trans)-nitrosation. We propose to use a comprehensive multimode imaging approach involving full spectral confocal and multiiphoton ..
  38. Red Blood Cells, Nitric Oxide and Pulmonary Circulation
    Steven Deem; Fiscal Year: 2008
    ..abstract_text> ..
  39. HYPOXIC PULMONARY VASOCANSTRICTION AND RED BLOOD CELLS
    Steven Deem; Fiscal Year: 2002
    ....
  40. ENDOGENOUS NITROSATION CHEMISTRY IN CARCINOGENESIS
    RICHARD LOEPPKY; Fiscal Year: 2004
    ..and practical strategies for the significant reduction of carcinogenesis which may arise from endogenous nitrosation processes which can generate electrophilic species capable of modifying DNA and causing mutations...
  41. CARCINOGENESIS--HIGHLY REACTIVE NITROSAMINES
    RICHARD LOEPPKY; Fiscal Year: 2001
    ....
  42. Mechanisms of Nitric Oxide Mediated Cell Injury
    DAVID JOURD HEUIL; Fiscal Year: 2005
    ..specifically, it has not yet been established to what extent functional alterations caused by NO-mediated S-nitrosation reactions (the addition of an NO+ group to a thiol residue) might contribute to cytostasis...
  43. NITRIC OXIDE, PLP ACYLATION & THE PATHOPHYSIOLOGY OF MS
    OSCAR BIZZOZERO; Fiscal Year: 2003
    ..abstract_text> ..
  44. HPLC DETECTION OF RADICAL INTERMEDIATES
    Detcho Stoyanovsky; Fiscal Year: 2002
    ..The method is expected to display several orders of magnitude higher sensitivity than then traditional ESR spin trapping technique and may be a new approach in assays of oxidative stress. ..
  45. The Dynamics of Nitric Oxide in the Vascular System
    Jack Lancaster; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  46. Nitrates, Nitrites, and Nitrosatable Drugs and Risk of Selected Birth Defects
    JEAN DIANE BRENDER; Fiscal Year: 2010
    ..We will also examine the effects of vitamins C and E (inhibitors of nitrosation) on the relations between nitrate/nitrite intake and nitrosatable drugs and risk of selected congenital ..
  47. NITROGEN AND OXYGEN RADICAL INTERACTIONS IN SURGERY
    Jack Lancaster; Fiscal Year: 2003
    ..By determining the phenotypic and genotypic changes in cells induced by this pre-exposure, the specific protective mechanisms which are upregulated in this defensive response will be identif d. ..
  48. Mechanisms of Biological Nitrosation from Nitric Oxide
    Jack Lancaster; Fiscal Year: 2006
    ..Among the biological actions of NO, nitrosation (the chemistry of the one-electron oxidized species nitrosonium (NO+)) has historically (and undoubtedly) ..
  49. Chemoprevention of Colonic NOC Formation and Action
    SIDNEY MIRVISH; Fiscal Year: 2006
    ..Aim 2: Because ascorbate inhibits acid-catalyzed gastric nitrosation, we will test reduction of fecal NOC level on feeding 3 ascorbate doses in diet with nitrite in water, and test ..
  50. Nitric oxide and mitochondrial biogenesis in sepsis
    Claude A Piantadosi; Fiscal Year: 2010
    ..abstract_text> ..
  51. MECHANISMS OF NO-MEDIATED PROTEIN S-NITROSYLATION IN EAE
    OSCAR BIZZOZERO; Fiscal Year: 2007
    ..abstract_text> ..
  52. Lung Injury Protection by Coagulation Blockade
    CLAUDE PIANTADOSI; Fiscal Year: 2008
    ..A successfully optimized strategy could greatly attenuate persistence of pulmonary inflammation and facilitate the resolution of human ARDS. ..
  53. Industrial Pollutants and Risk of Non-Hodgkins Lymphoma
    Anneclaire De Roos; Fiscal Year: 2006
    ..abstract_text> ..
  54. The Pathogenesis and Genetics of Environmental Asthma
    JONATHAN STAMLER; Fiscal Year: 2007
    ..In aggregate, the coupled scientific findings in this program will substantially enhance our understanding of the pathogenesis and genetics of asthma. ..
  55. CARCINOGENIC CYCLIC NITROSAMINE DNA ADDUCTS
    Stephen S Hecht; Fiscal Year: 2010
    ..The proposed research is significant because of the known human exposure to these carcinogens through the diet, tobacco products, and endogenous formation. ..
  56. Catalytic Processes for Selective Organic Synthesis
    GARY MOLANDER; Fiscal Year: 2005
    ..4) Carbometalation/cyclization reactions will be explored utilizing organochromium species. 5) Ligands and catalytic systems designed for asymmetric synthesis will be explored. ..
  57. Agilent 5973 GC/MS System
    GARY MOLANDER; Fiscal Year: 2002
    ..Our current lack of such instrumentation negatively affects the ability to carry out many of these studies. This critical capability, in the form of an instrument for everyday analysis, is urgently needed. ..
  58. Immune Responsiveness, APOE/Gender in Neurodegeneration
    Carol Colton; Fiscal Year: 2008
    ..Thus, we will examine the role of hormones in the regulation of microglial and peritoneal macrophage immune activation in mice expressing only human apoE3 protein or expressing only human apoE4 protein. ..
  59. Dihydrolipoamide Acyltransferase:Target for Chemotherapy
    Carl Nathan; Fiscal Year: 2004
    ..abstract_text> ..
  60. MYCOBACTERIAL RESISTANCE TO REACTIVE NITROGEN/OXYGEN
    Carl Nathan; Fiscal Year: 2003
    ....
  61. Regulation of Microglial Activation
    Carol Colton; Fiscal Year: 2003
    ....