methylation

Summary

Summary: Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)

Top Publications

  1. ncbi Translating the histone code
    T Jenuwein
    Research Institute of Molecular Pathology IMP at the Vienna Biocenter, Dr Bohrgasse 7, A 1030 Vienna, Austria
    Science 293:1074-80. 2001
  2. pmc Genomewide analysis of PRC1 and PRC2 occupancy identifies two classes of bivalent domains
    Manching Ku
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA, USA
    PLoS Genet 4:e1000242. 2008
  3. pmc Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
    Tarjei S Mikkelsen
    Broad Institute of Harvard and MIT, Cambridge, Massachusetts 02142, USA
    Nature 448:553-60. 2007
  4. pmc Protein arginine methylation in mammals: who, what, and why
    Mark T Bedford
    The University of Texas M D Anderson Cancer Center, Science Park Research Division, P O Box 389, Smithville, TX 78957, USA
    Mol Cell 33:1-13. 2009
  5. pmc Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions
    Adrian P Bracken
    Biotech Research and Innovation Centre BRIC, 2100 Copenhagen Ø, Denmark
    Genes Dev 20:1123-36. 2006
  6. pmc Combinatorial patterns of histone acetylations and methylations in the human genome
    Zhibin Wang
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Genet 40:897-903. 2008
  7. pmc Differential chromatin marking of introns and expressed exons by H3K36me3
    Paulina Kolasinska-Zwierz
    The Gurdon Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nat Genet 41:376-81. 2009
  8. ncbi A bivalent chromatin structure marks key developmental genes in embryonic stem cells
    Bradley E Bernstein
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Cell 125:315-26. 2006
  9. doi Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers
    Michiel Vermeulen
    Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    Cell 142:967-80. 2010
  10. ncbi LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription
    Eric Metzger
    Universitäts Frauenklinik und Zentrum für Klinische Forschung, Klinikum der Universität Freiburg, Breisacherstrasse 66, 79106 Freiburg, Germany
    Nature 437:436-9. 2005

Research Grants

  1. Variation in M. tuberculosis in response to host selection
    Sarah Fortune; Fiscal Year: 2009
  2. High Throughput Genotyping and DNA Sequencing for Studying the Genetic Contributi
    Sonia Dua; Fiscal Year: 2013
  3. Yun Fai Chris Lau; Fiscal Year: 2014
  4. Tibor T Glant; Fiscal Year: 2016
  5. Carole R Mendelson; Fiscal Year: 2016
  6. Discovery of Small Molecule MBT Domain Antagonists
    STEPHEN VERNON FRYE; Fiscal Year: 2010
  7. Jian Min Yuan; Fiscal Year: 2014
  8. Neil W Kowall; Fiscal Year: 2014
  9. Lung Lysyl Oxidase Regulation by Metal Ion Homeostasis
    Wande Li; Fiscal Year: 2013
  10. MOLECULAR BASIS OF INSULIN RESISTANCE
    Maureen J Charron; Fiscal Year: 2010

Detail Information

Publications372 found, 100 shown here

  1. ncbi Translating the histone code
    T Jenuwein
    Research Institute of Molecular Pathology IMP at the Vienna Biocenter, Dr Bohrgasse 7, A 1030 Vienna, Austria
    Science 293:1074-80. 2001
    ....
  2. pmc Genomewide analysis of PRC1 and PRC2 occupancy identifies two classes of bivalent domains
    Manching Ku
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA, USA
    PLoS Genet 4:e1000242. 2008
    In embryonic stem (ES) cells, bivalent chromatin domains with overlapping repressive (H3 lysine 27 tri-methylation) and activating (H3 lysine 4 tri-methylation) histone modifications mark the promoters of more than 2,000 genes...
  3. pmc Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
    Tarjei S Mikkelsen
    Broad Institute of Harvard and MIT, Cambridge, Massachusetts 02142, USA
    Nature 448:553-60. 2007
    ..This study provides a framework for the application of comprehensive chromatin profiling towards characterization of diverse mammalian cell populations...
  4. pmc Protein arginine methylation in mammals: who, what, and why
    Mark T Bedford
    The University of Texas M D Anderson Cancer Center, Science Park Research Division, P O Box 389, Smithville, TX 78957, USA
    Mol Cell 33:1-13. 2009
    ..A small family of gene products that catalyze such methylation reactions in eukaryotes (PRMTs) works in conjunction with a changing cast of associated subunits to recognize ..
  5. pmc Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions
    Adrian P Bracken
    Biotech Research and Innovation Centre BRIC, 2100 Copenhagen Ø, Denmark
    Genes Dev 20:1123-36. 2006
    ....
  6. pmc Combinatorial patterns of histone acetylations and methylations in the human genome
    Zhibin Wang
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Genet 40:897-903. 2008
    ..Our data suggest that these histone modifications may act cooperatively to prepare chromatin for transcriptional activation...
  7. pmc Differential chromatin marking of introns and expressed exons by H3K36me3
    Paulina Kolasinska-Zwierz
    The Gurdon Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nat Genet 41:376-81. 2009
    ..We propose that H3K36me3 exon marking in chromatin provides a dynamic link between transcription and splicing...
  8. ncbi A bivalent chromatin structure marks key developmental genes in embryonic stem cells
    Bradley E Bernstein
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Cell 125:315-26. 2006
    ..We explored this by examining histone methylation in mouse embryonic stem (ES) cells across 56 large HCNE-rich loci...
  9. doi Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers
    Michiel Vermeulen
    Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    Cell 142:967-80. 2010
    ..The ORC complex, including LRWD1, binds to the three most prominent transcriptional repressive lysine methylation sites...
  10. ncbi LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription
    Eric Metzger
    Universitäts Frauenklinik und Zentrum für Klinische Forschung, Klinikum der Universität Freiburg, Breisacherstrasse 66, 79106 Freiburg, Germany
    Nature 437:436-9. 2005
    ..Gene activation and repression is specifically regulated by histone methylation status at distinct lysine residues...
  11. pmc 2'-O methylation of the viral mRNA cap evades host restriction by IFIT family members
    Stephane Daffis
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nature 468:452-6. 2010
    ..Whereas N-7 methylation is essential for RNA translation and stability, the function of 2'-O methylation has remained uncertain since ..
  12. ncbi Methylation of lysine 4 on histone H3: intricacy of writing and reading a single epigenetic mark
    Alexander J Ruthenburg
    Laboratory of Chromatin Biology, The Rockefeller University, New York, NY 10021, USA
    Mol Cell 25:15-30. 2007
    ..Here, we focus on one such element of variation: methylation of lysine 4 in histone H3 (H3K4)...
  13. pmc Long noncoding RNA as modular scaffold of histone modification complexes
    Miao Chih Tsai
    Howard Hughes Medical Institute and Program in Epithelial Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Science 329:689-93. 2010
    ..of PRC2 and LSD1 and coordinates targeting of PRC2 and LSD1 to chromatin for coupled histone H3 lysine 27 methylation and lysine 4 demethylation...
  14. ncbi The diverse functions of histone lysine methylation
    Cyrus Martin
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina 27599 7295, USA
    Nat Rev Mol Cell Biol 6:838-49. 2005
    ..One such modification, lysine methylation, has important functions in many biological processes that include heterochromatin formation, X-chromosome ..
  15. pmc Pharmacologic disruption of Polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells
    Jing Tan
    Molecular Pharmacology, Genome Institute of Singapore, 138672, Singapore
    Genes Dev 21:1050-63. 2007
    Polycomb-repressive complex 2 (PRC2)-mediated histone methylation plays an important role in aberrant cancer gene silencing and is a potential target for cancer therapy...
  16. pmc Histone methylation: a dynamic mark in health, disease and inheritance
    Eric L Greer
    Cell Biology Department, Harvard Medical School and Division of Newborn Medicine, Children s Hospital Boston, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Rev Genet 13:343-57. 2012
    ..Post-translational modification of histones by methylation is an important and widespread type of chromatin modification that is known to influence biological processes ..
  17. ncbi UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development
    Karl Agger
    Biotech Research and Innovation Centre BRIC, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen, Denmark
    Nature 449:731-4. 2007
    ..The polycomb repressive complex 2 (PRC2) mediates transcriptional repression by catalysing the di- and tri-methylation of Lys 27 on histone H3 (H3K27me2/me3)...
  18. pmc Chromatin signatures in multipotent human hematopoietic stem cells indicate the fate of bivalent genes during differentiation
    Kairong Cui
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Cell Stem Cell 4:80-93. 2009
    ..Thus, our data suggest that gene expression changes during differentiation are programmed by chromatin modifications present at the HSC/HPC stage and provide a resource for enhancer and promoter identification...
  19. pmc Methylation as a crucial step in plant microRNA biogenesis
    Bin Yu
    Waksman Institute, Rutgers University, Piscataway, NJ 08854, USA
    Science 307:932-5. 2005
    b>Methylation on the base or the ribose is prevalent in eukaryotic ribosomal RNAs (rRNAs) and is thought to be crucial for ribosome biogenesis and function...
  20. ncbi The complex language of chromatin regulation during transcription
    Shelley L Berger
    The Wistar Institute, 3601 Spruce Street, Room 201, Philadelphia, Pennsylvania 19104, USA
    Nature 447:407-12. 2007
    ..development in understanding the influence of chromatin on gene regulation has been the finding that DNA methylation and histone post-translational modifications lead to the recruitment of protein complexes that regulate ..
  21. doi Spp1, a member of the Set1 Complex, promotes meiotic DSB formation in promoters by tethering histone H3K4 methylation sites to chromosome axes
    Vérane Sommermeyer
    Institut Curie, Centre de Recherche, 75248 Paris, France
    Mol Cell 49:43-54. 2013
    ..The PHD finger module of Spp1, which reads H3K4 methylation close to promoters, promotes DSB formation by tethering these regions to chromosome axes and activating ..
  22. pmc Recognition of trimethylated histone H3 lysine 4 facilitates the recruitment of transcription postinitiation factors and pre-mRNA splicing
    Robert J Sims
    Department of Biochemistry, New York University School of Medicine, New York, NY 10016, USA
    Mol Cell 28:665-76. 2007
    ..These findings suggest that methylated H3K4 serves to facilitate the competency of pre-mRNA maturation through the bridging of spliceosomal components to H3K4me3 via CHD1...
  23. ncbi Arginine methylation an emerging regulator of protein function
    Mark T Bedford
    Department of Carcinogenesis, The University of Texas M D Anderson Cancer Center, Smithville, Texas 78957, USA
    Mol Cell 18:263-72. 2005
    Arginine methylation is now coming out of the shadows of protein phosphorylation and entering the mainstream, largely due to the identification of the family of enzymes that lay down this modification...
  24. ncbi Histone H3 methylation by Set2 directs deacetylation of coding regions by Rpd3S to suppress spurious intragenic transcription
    Michael J Carrozza
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Cell 123:581-92. 2005
    ..This erases transcription elongation-associated acetylation to suppress intragenic transcription initiation...
  25. pmc The Dnmt3a PWWP domain reads histone 3 lysine 36 trimethylation and guides DNA methylation
    Arunkumar Dhayalan
    Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, Bremen, Germany
    J Biol Chem 285:26114-20. 2010
    ..H3K36me3 is known to be a repressive modification correlated with DNA methylation in mammals and heterochromatin in Schizosaccharomyces pombe...
  26. pmc Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair
    Maria Victoria Botuyan
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Cell 127:1361-73. 2006
    Histone lysine methylation has been linked to the recruitment of mammalian DNA repair factor 53BP1 and putative fission yeast homolog Crb2 to DNA double-strand breaks (DSBs), but how histone recognition is achieved has not been ..
  27. pmc EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency
    Xiaohua Shen
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 32:491-502. 2008
    ..Analysis of Ezh2(-/-) ESCs suggests existence of an additional enzyme(s) catalyzing H3K27 methylation. We have identified EZH1, a homolog of EZH2 that is physically present in a noncanonical PRC2 complex, as an ..
  28. doi RNA nucleotide methylation
    Yuri Motorin
    Laboratoire ARN RNP Maturation Structure Fonction, Enzymologie Moléculaire et Structurale AREMS, Universite Henri Poincare, Vandoeuvre les Nancy, France
    Wiley Interdiscip Rev RNA 2:611-31. 2011
    b>Methylation of RNA occurs at a variety of atoms, nucleotides, sequences and tertiary structures...
  29. ncbi MLL targets SET domain methyltransferase activity to Hox gene promoters
    Thomas A Milne
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Mol Cell 10:1107-17. 2002
    ..This methylase activity is associated with Hox gene activation and H3 (Lys4) methylation at cis-regulatory sequences in vivo...
  30. pmc The Polycomb complex PRC2 and its mark in life
    Raphael Margueron
    Institut Curie, 26 Rue d Ulm, 75005 Paris, France
    Nature 469:343-9. 2011
    ..PRC2 also contributes to chromatin compaction, and catalyses the methylation of histone H3 at lysine 27...
  31. pmc Genetic and functional diversification of small RNA pathways in plants
    Zhixin Xie
    Center for Gene Research and Biotechnology and Department of Botany and Plant Pathology, Oregon State University, Corvallis, USA
    PLoS Biol 2:E104. 2004
    ..We conclude that proliferation and diversification of DCL and RDR genes during evolution of plants contributed to specialization of small RNA-directed pathways for development, chromatin structure, and defense...
  32. ncbi MLL translocations, histone modifications and leukaemia stem-cell development
    Andrei V Krivtsov
    Division of Haematology Oncology, Children s Hospital, Department of Pediatric Oncology, and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Rev Cancer 7:823-33. 2007
    ..The link between a chromatin modulator and leukaemia stem cells provides support for epigenetic landscapes as an important part of leukaemia and normal stem-cell development...
  33. pmc 2'-O methylation of the viral mRNA cap by West Nile virus evades ifit1-dependent and -independent mechanisms of host restriction in vivo
    Kristy J Szretter
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri, United Stated of America
    PLoS Pathog 8:e1002698. 2012
    ..pathogenesis in vivo of wild type WNV (WNV-WT) and a mutant in the NS5 gene (WNV-E218A) lacking 2'-O methylation of the 5' viral RNA cap...
  34. ncbi Selective anchoring of TFIID to nucleosomes by trimethylation of histone H3 lysine 4
    Michiel Vermeulen
    Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    Cell 131:58-69. 2007
    ..Our experiments reveal crosstalk between histone modifications and the transcription factor TFIID. This has important implications for regulation of RNA polymerase II-mediated transcription in higher eukaryotes...
  35. pmc Covalent histone modifications--miswritten, misinterpreted and mis-erased in human cancers
    Ping Chi
    Laboratory of Chromatin Biology and Epigenetics, 1230 York Avenue, The Rockefeller University, Box 78, New York, NY 10065, USA
    Nat Rev Cancer 10:457-69. 2010
    ..by plant homeodomain finger-containing proteins, and how misreading, miswriting and mis-erasing of histone methylation marks can be associated with oncogenesis and progression...
  36. pmc Nucleosome-interacting proteins regulated by DNA and histone methylation
    Till Bartke
    The Gurdon Institute, Department of Pathology, Cambridge, UK
    Cell 143:470-84. 2010
    ..Our analysis reveals proteins whose binding to nucleosomes is regulated by methylation of CpGs, H3K4, H3K9, and H3K27 or a combination thereof...
  37. pmc MMSET regulates histone H4K20 methylation and 53BP1 accumulation at DNA damage sites
    Huadong Pei
    Division of Oncology Research, Mayo Clinic, Rochester, Minnesota 55905, USA
    Nature 470:124-8. 2011
    ..Here we found that H4K20 methylation actually increases locally upon the induction of DSBs and that methylation of H4K20 at DSBs is mediated by the ..
  38. doi Comprehensive genome methylation analysis in bladder cancer: identification and validation of novel methylated genes and application of these as urinary tumor markers
    Thomas Reinert
    Department of Molecular Medicine and Urology, Aarhus University Hospital, Aarhus N, Denmark
    Clin Cancer Res 17:5582-92. 2011
    Epigenetic alterations are common and can now be addressed in a parallel fashion. We investigated the methylation in bladder cancer with respect to location in genome, consistency, variation in metachronous tumors, impact on transcripts, ..
  39. pmc PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex
    Young Wook Cho
    Nuclear Receptor Biology Section, Clinical Endocrinology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 282:20395-406. 2007
    ..As histone H3 K4 methylation associates with active genes, our study suggests a potential role of PTIP in the regulation of gene expression.
  40. pmc PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression
    Wen Liu
    Howard Hughes Medical Institute, School of Medicine, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Nature 466:508-12. 2010
    ..Thus, the identification and characterization of an H4K20me1 demethylase, PHF8, has revealed an intimate link between this enzyme and two distinct events in cell cycle progression...
  41. ncbi Role for DNA methylation in genomic imprinting
    E Li
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142
    Nature 366:362-5. 1993
    ..The study of transgene methylation has shown that methylation patterns can be inherited in a parent-of-origin-specific manner, suggesting that DNA ..
  42. pmc Methylation protects miRNAs and siRNAs from a 3'-end uridylation activity in Arabidopsis
    Junjie Li
    Department of Botany and Plant Sciences, Institute of Integrative Genome Research, University of California, Riverside, Riverside, California 92508, USA
    Curr Biol 15:1501-7. 2005
    ..a miRNA methyltransferase that adds a methyl group to the 3'-most nucleotide of miRNAs, but the role of miRNA methylation was unknown...
  43. pmc N6-methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO
    Guifang Jia
    Department of Chemistry, University of Chicago, Chicago, Illinois, USA
    Nat Chem Biol 7:885-7. 2011
    ..We further show the partial colocalization of FTO with nuclear speckles, which supports the notion that m(6)A in nuclear RNA is a major physiological substrate of FTO...
  44. pmc Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons
    Kate D Meyer
    Department of Pharmacology, Weill Medical College, Cornell University, New York, NY 10065, USA
    Cell 149:1635-46. 2012
    b>Methylation of the N(6) position of adenosine (m(6)A) is a posttranscriptional modification of RNA with poorly understood prevalence and physiological relevance...
  45. pmc Histone lysine methylation dynamics: establishment, regulation, and biological impact
    Joshua C Black
    Massachusetts General Hospital Cancer Center and Department of Medicine, Harvard Medical School, 13 th Street, Charlestown, MA 02129, USA
    Mol Cell 48:491-507. 2012
    Histone lysine methylation has emerged as a critical player in the regulation of gene expression, cell cycle, genome stability, and nuclear architecture...
  46. doi A ncRNA modulates histone modification and mRNA induction in the yeast GAL gene cluster
    Jonathan Houseley
    Wellcome Trust Centre for Cell Biology, Institute for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Mol Cell 32:685-95. 2008
    ..These chromatin modifications act principally through the Rpd3S complex to aid glucose repression of GAL1-10 at physiologically relevant sugar concentrations...
  47. pmc Molecular coupling of DNA methylation and histone methylation
    Hideharu Hashimoto
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Epigenomics 2:657-69. 2010
    ..DNA methylation is associated with histone modifications, particularly the absence of histone H3 lysine 4 methylation (H3K4me0) ..
  48. pmc MLL-rearranged leukemia is dependent on aberrant H3K79 methylation by DOT1L
    Kathrin M Bernt
    Division of Hematology Oncology, Children s Hospital, Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 20:66-78. 2011
    ..Suppression of MLL translocation-associated gene expression corresponded with dependence of MLL-AF9 leukemia on Dot1l in vivo. These data point to DOT1L as a potential therapeutic target in MLL-rearranged leukemia...
  49. ncbi Double chromodomains cooperate to recognize the methylated histone H3 tail
    John F Flanagan
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia 22908, USA
    Nature 438:1181-5. 2005
    ..Furthermore, unique inserts within chromodomain 1 of CHD1 block the expected site of H3 tail binding seen in HP1 and Polycomb, instead directing H3 binding to a groove at the inter-chromodomain junction...
  50. pmc SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins
    David C Schultz
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Genes Dev 16:919-32. 2002
    ..Recent genetic and biochemical studies link H3-lysine 9 (H3-K9) methylation to HP1-mediated heterochromatin formation and gene silencing...
  51. ncbi Role of histone H3 lysine 27 methylation in Polycomb-group silencing
    Ru Cao
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Science 298:1039-43. 2002
    ..Recent studies have implicated histone methylation in long-term gene silencing...
  52. pmc ncRNA- and Pc2 methylation-dependent gene relocation between nuclear structures mediates gene activation programs
    Liuqing Yang
    Howard Hughes Medical Institute, University of California, San Diego, School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093 0648, USA
    Cell 147:773-88. 2011
    ..Here, we report that methylation/demethylation of Polycomb 2 protein (Pc2) controls relocation of growth-control genes between Polycomb bodies (..
  53. pmc Two distinct repressive mechanisms for histone 3 lysine 4 methylation through promoting 3'-end antisense transcription
    Thanasis Margaritis
    Molecular Cancer Research, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS Genet 8:e1002952. 2012
    ..We have investigated the roles of H3K4 methylation in Saccharomyces cerevisiae by determining genome-wide expression-profiles of mutants in the Set1 complex, ..
  54. doi Lysine methylation: beyond histones
    Xi Zhang
    Department of Biochemistry and Molecular Biology, University of Texas MD Anderson Cancer Center, Houston, 77030, USA
    Acta Biochim Biophys Sin (Shanghai) 44:14-27. 2012
    Posttranslational modifications (PTMs) of histone proteins, such as acetylation, methylation, phosphorylation, and ubiquitylation, play essential roles in regulating chromatin dynamics...
  55. doi Symmetric dimethylation of H3R2 is a newly identified histone mark that supports euchromatin maintenance
    Valentina Migliori
    Institute of Molecular and Cell Biology, Singapore
    Nat Struct Mol Biol 19:136-44. 2012
    ..Our findings identify H3R2me2s as a previously unknown mark that keeps genes poised in euchromatin for transcriptional activation upon cell-cycle withdrawal and differentiation in human cells...
  56. pmc Epigenetics of the depressed brain: role of histone acetylation and methylation
    Haosheng Sun
    Fishberg Department of Neuroscience and Friedman Brain Institute, School of Medicine, New York, NY, USA
    Neuropsychopharmacology 38:124-37. 2013
    ..our understanding of epigenetic contributions to depression, in particular the role of histone acetylation and methylation, which are revealing novel mechanistic insight into the syndrome that may aid in the development of novel ..
  57. doi Step-wise methylation of histone H3K9 positions heterochromatin at the nuclear periphery
    Benjamin D Towbin
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland
    Cell 150:934-47. 2012
    ..In a genome-wide RNAi screen, we found that depletion of S-adenosylmethionine (SAM) synthetase reduces histone methylation globally and causes derepression and release of heterochromatin from the nuclear periphery in Caenorhabditis ..
  58. pmc The Polycomb Ezh2 methyltransferase regulates muscle gene expression and skeletal muscle differentiation
    Giuseppina Caretti
    Muscle Gene Expression Group, Laboratory of Muscle Biology, NIAMS, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 18:2627-38. 2004
    ..Their presence correlated with methylation of K27 of histone H3...
  59. ncbi Histone demethylation mediated by the nuclear amine oxidase homolog LSD1
    Yujiang Shi
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Cell 119:941-53. 2004
    ..acetylation is determined by both acetyltransferases and deacetylases, it has been unclear whether histone methylation is also regulated by enzymes with opposing activities...
  60. pmc Genome-wide remodeling of the epigenetic landscape during myogenic differentiation
    Patrik Asp
    Department of Pathology and Cancer Institute, New York University School of Medicine, 522 First Avenue, Smilow Research Building 1104, New York, NY 10016, USA
    Proc Natl Acad Sci U S A 108:E149-58. 2011
    ..Next, we examined the role of Polycomb-mediated H3K27 methylation in gene repression by systematically ablating components of both PRC1 and PRC2 complexes...
  61. ncbi WDR5 associates with histone H3 methylated at K4 and is essential for H3 K4 methylation and vertebrate development
    Joanna Wysocka
    Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
    Cell 121:859-72. 2005
    Histone H3 lysine 4 (K4) methylation has been linked to the transcriptional activation in a variety of eukaryotic species...
  62. ncbi Polycomb-mediated methylation on Lys27 of histone H3 pre-marks genes for de novo methylation in cancer
    Yeshayahu Schlesinger
    Department of Cellular Biochemistry and Human Genetics, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Nat Genet 39:232-6. 2007
    Many genes associated with CpG islands undergo de novo methylation in cancer...
  63. doi KAP1 controls endogenous retroviruses in embryonic stem cells
    Helen M Rowe
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Nature 463:237-40. 2010
    ..ERVs are transcriptionally silenced during early embryogenesis by histone and DNA methylation (and reviewed in ref...
  64. ncbi Histone modifications in transcriptional regulation
    Shelley L Berger
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Curr Opin Genet Dev 12:142-8. 2002
    ..Research in the past two years reveals these modifications to consist of phosphorylation, methylation and ubiquitination, in addition to the better-characterized acetylation...
  65. ncbi Demethylation of H3K27 regulates polycomb recruitment and H2A ubiquitination
    Min Gyu Lee
    Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Science 318:447-50. 2007
    b>Methylation of histone H3 lysine 27 (H3K27) is a posttranslational modification that is highly correlated with genomic silencing...
  66. pmc Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands
    J G Herman
    Oncology Center, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 93:9821-6. 1996
    Precise mapping of DNA methylation patterns in CpG islands has become essential for understanding diverse biological processes such as the regulation of imprinted genes, X chromosome inactivation, and tumor suppressor gene silencing in ..
  67. doi Set2 methylation of histone H3 lysine 36 suppresses histone exchange on transcribed genes
    Swaminathan Venkatesh
    Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, Missouri 64110, USA
    Nature 489:452-5. 2012
    Set2-mediated methylation of histone H3 at Lys 36 (H3K36me) is a co-transcriptional event that is necessary for the activation of the Rpd3S histone deacetylase complex, thereby maintaining the coding region of genes in a hypoacetylated ..
  68. pmc High-resolution mapping reveals a conserved, widespread, dynamic mRNA methylation program in yeast meiosis
    Schraga Schwartz
    Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
    Cell 155:1409-21. 2013
    ..We validated eight out of eight methylation sites in different genes with direct genetic analysis, demonstrated that methylated sites are significantly ..
  69. ncbi Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly
    J Nakayama
    Cold Spring Harbor Laboratory, Post Office Box 100, Cold Spring Harbor, NY 11724, USA
    Science 292:110-3. 2001
    ..Both the conserved chromo- and SET domains of Clr4 are required for H3 Lys9 methylation in vivo...
  70. ncbi Akt-mediated phosphorylation of EZH2 suppresses methylation of lysine 27 in histone H3
    Tai Lung Cha
    Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Science 310:306-10. 2005
    ..Our results imply that Akt regulates the methylation activity, through phosphorylation of EZH2, which may contribute to oncogenesis.
  71. pmc Promoter CpG methylation contributes to ES cell gene regulation in parallel with Oct4/Nanog, PcG complex, and histone H3 K4/K27 trimethylation
    Shaun D Fouse
    Department of Human Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA
    Cell Stem Cell 2:160-9. 2008
    We report here genome-wide mapping of DNA methylation patterns at proximal promoter regions in mouse embryonic stem (mES) cells. Most methylated genes are differentiation associated and repressed in mES cells...
  72. pmc A chromatin-wide transition to H4K20 monomethylation impairs genome integrity and programmed DNA rearrangements in the mouse
    Gunnar Schotta
    Research Institute of Molecular Pathology IMP, The Vienna Biocenter, A 1030 Vienna, Austria
    Genes Dev 22:2048-61. 2008
    H4K20 methylation is a broad chromatin modification that has been linked with diverse epigenetic functions...
  73. pmc The RING finger protein MSL2 in the MOF complex is an E3 ubiquitin ligase for H2B K34 and is involved in crosstalk with H3 K4 and K79 methylation
    Lipeng Wu
    Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Cell 43:132-44. 2011
    ..H2B K34ub by MSL1/2 directly regulates H3 K4 and K79 methylation through trans-tail crosstalk both in vitro and in cells...
  74. doi Splicing enhances recruitment of methyltransferase HYPB/Setd2 and methylation of histone H3 Lys36
    Sérgio Fernandes de Almeida
    Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal
    Nat Struct Mol Biol 18:977-83. 2011
    ..Genome-wide analysis of histone methylation in human cell lines and mouse primary T cells reveals that intron-containing genes are preferentially marked ..
  75. pmc Molecular basis for site-specific read-out of histone H3K4me3 by the BPTF PHD finger of NURF
    Haitao Li
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 442:91-5. 2006
    ..A major challenge in chromatin biology has centred on efforts to define the connection between specific methylation states and distinct biological read-outs impacting on function...
  76. pmc RNA methylation by Dnmt2 protects transfer RNAs against stress-induced cleavage
    Matthias Schaefer
    Division of Epigenetics, DKFZ ZMBH Alliance, German Cancer Research Center, Heidelberg 69120, Germany
    Genes Dev 24:1590-5. 2010
    ..Strikingly, stress-induced cleavage of tRNAs was Dnmt2-dependent, and Dnmt2-mediated methylation protected tRNAs against ribonuclease cleavage...
  77. doi Histone methylation by PRC2 is inhibited by active chromatin marks
    Frank W Schmitges
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH 4058 Basel, Switzerland
    Mol Cell 42:330-41. 2011
    ..Inhibition by active chromatin marks, coupled to stimulation by transcriptionally repressive H3K27me3, enables PRC2 to autonomously template repressive H3K27me3 without overwriting active chromatin domains...
  78. pmc Histone H3 lysine 4 trimethylation marks meiotic recombination initiation sites
    Valerie Borde
    Institut Curie Centre de Recherche, UMR7147 CNRS, Universite Pierre et Marie Curie, Paris, France
    EMBO J 28:99-111. 2009
    ..Novel perspectives to dissect the various layers of the controls of meiotic DSB formation are discussed...
  79. doi Biochemical mechanisms of gene regulation by polycomb group protein complexes
    Jurg Muller
    EMBL, Gene Expression Programme, Heidelberg, Germany
    Curr Opin Genet Dev 19:150-8. 2009
    ..Rather, PcG mediated repression also constitutes a mechanism for dynamic control of gene transcription...
  80. pmc Arabidopsis TFL2/LHP1 specifically associates with genes marked by trimethylation of histone H3 lysine 27
    Franziska Turck
    Abteilung Entwicklungsbiologie der Pflanzen, Max Planck Institut fur Zuchtungsforschung, Cologne, Germany
    PLoS Genet 3:e86. 2007
    ..Rather, our data suggest that TFL2/LHP1 recognizes specifically H3K27me3 in vivo as part of a mechanism that represses the expression of many genes targeted by PRC2...
  81. doi A selective inhibitor of EZH2 blocks H3K27 methylation and kills mutant lymphoma cells
    Sarah K Knutson
    Epizyme, Inc, Cambridge, MA, USA
    Nat Chem Biol 8:890-6. 2012
    ..The compound reduces H3K27 methylation in various lymphoma cells; this translates into apoptotic cell killing in heterozygous Tyr641 or Ala677 mutant ..
  82. ncbi Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain
    A J Bannister
    Wellcome CRC Institute and Department of Pathology, University of Cambridge, UK
    Nature 410:120-4. 2001
    ..This model may also explain the stable inheritance of the heterochromatic state...
  83. pmc Target RNA-directed trimming and tailing of small silencing RNAs
    Stefan L Ameres
    Howard Hughes Medical Institute and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 328:1534-9. 2010
    ..Target complementarity also affects small RNA stability in human cells. These results provide an explanation for the partial complementarity between animal miRNAs and their targets...
  84. pmc Integrated genetic and epigenetic analysis identifies haplotype-specific methylation in the FTO type 2 diabetes and obesity susceptibility locus
    Christopher G Bell
    Medical Genomics, UCL Cancer Institute, University College London, London, United Kingdom
    PLoS ONE 5:e14040. 2010
    ..We assayed DNA methylation in 60 females, stratified according to disease susceptibility haplotype using previously identified association ..
  85. pmc Ribose 2'-O-methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5
    Roland Zust
    Institute of Immunobiology, Kantonal Hospital St Gallen, St Gallen, Switzerland
    Nat Immunol 12:137-43. 2011
    The 5' cap structures of higher eukaryote mRNAs have ribose 2'-O-methylation. Likewise, many viruses that replicate in the cytoplasm of eukaryotes have evolved 2'-O-methyltransferases to autonomously modify their mRNAs...
  86. ncbi Covalent modifications of histones during development and disease pathogenesis
    Sukesh R Bhaumik
    Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, Illinois 62901, USA
    Nat Struct Mol Biol 14:1008-16. 2007
    ..involved in four different types of histone modification: acetylation, ubiquitination, phosphorylation and methylation. We also discuss how perturbation of the activity of this enzymatic machinery can cause developmental defects ..
  87. pmc H3 lysine 4 di- and tri-methylation deposited by cryptic transcription attenuates promoter activation
    Marina Pinskaya
    Centre de Genetique Moleculaire CNRS, Universite Pierre et Marie Curie, Gif sur Yvette, France
    EMBO J 28:1697-707. 2009
    Set1-dependent H3K4 di- and tri-methylation (H3K4me2/3) have been associated with active transcription. Recent data indicate that the H3K4me2/3 also plays a poorly characterized RNA-dependent repressive role...
  88. ncbi High-resolution profiling of histone methylations in the human genome
    Artem Barski
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892, USA
    Cell 129:823-37. 2007
    ..H2A.Z associates with functional regulatory elements, and CTCF marks boundaries of histone methylation domains. Chromosome banding patterns are correlated with unique patterns of histone modifications...
  89. pmc Correlated alterations in genome organization, histone methylation, and DNA-lamin A/C interactions in Hutchinson-Gilford progeria syndrome
    Rachel Patton McCord
    Program in Systems Biology, Department of Biochemistry and Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Genome Res 23:260-9. 2013
    ..These changes may result in transcriptional misregulation and eventually trigger the global loss of spatial chromatin compartmentalization in late passage HGPS fibroblasts...
  90. ncbi Methylation of H3-lysine 79 is mediated by a new family of HMTases without a SET domain
    Qin Feng
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 27599, USA
    Curr Biol 12:1052-8. 2002
    The N-terminal tails of core histones are subjected to multiple covalent modifications, including acetylation, methylation, and phosphorylation...
  91. ncbi A histone H3 methyltransferase controls epigenetic events required for meiotic prophase
    Katsuhiko Hayashi
    Department of Molecular Embryology, Research Institute, Osaka Medical Center for Maternal and Child Health, Murodo cho 840, Izumi, Osaka 594 1101, Japan
    Nature 438:374-8. 2005
    ..but not mono- or dimethylation, of lysine 4 of histone H3, and a transactivation activity that depends on its methylation activity...
  92. ncbi p53 is regulated by the lysine demethylase LSD1
    Jing Huang
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 449:105-8. 2007
    ..and transcriptional activator, is regulated by numerous post-translational modifications, including lysine methylation. Histone lysine methylation has recently been shown to be reversible; however, it is not known whether non-..
  93. ncbi Methylation of histone H3R2 by PRMT6 and H3K4 by an MLL complex are mutually exclusive
    Ernesto Guccione
    Department of Experimental Oncology, European Institute of Oncology IEO, IFOM IEO Campus, Milan 20139, Italy
    Nature 449:933-7. 2007
    ..We previously observed that asymmetric di-methylation of histone H3 arginine 2 (H3R2me2a) counter-correlates with di- and tri- methylation of H3 lysine 4 (H3K4me2, ..
  94. pmc Ezh2 orchestrates gene expression for the stepwise differentiation of tissue-specific stem cells
    Elena Ezhkova
    Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, NY 10065, USA
    Cell 136:1122-35. 2009
    ..They maintain their proliferative potential and globally repressing undesirable differentiation programs while selectively establishing a specific terminal differentiation program in a stepwise fashion...
  95. pmc Dimethylation of H3K4 by Set1 recruits the Set3 histone deacetylase complex to 5' transcribed regions
    Taesoo Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 137:259-72. 2009
    ....
  96. pmc Yeast targets for mRNA methylation
    Zsuzsanna Bodi
    School of Biosciences, Plant Sciences Division, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, UK
    Nucleic Acids Res 38:5327-35. 2010
    ..We demonstrate that in S. cerevisiae, substantial levels of internal adenosine methylation are present in the GpA context in mRNA from sporulating cells, which is consistent with the preferred ..
  97. pmc Amplification of siRNA in Caenorhabditis elegans generates a transgenerational sequence-targeted histone H3 lysine 9 methylation footprint
    Sam Guoping Gu
    Department of Pathology, Stanford University School of Medicine, Stanford, California, USA
    Nat Genet 44:157-64. 2012
    ..These results implicate dsRNA-triggered chromatin modification in C. elegans as a programmable and locus-specific response defining a metastable state that can persist through generational boundaries...
  98. doi Cell cycle control of centromeric repeat transcription and heterochromatin assembly
    Ee Sin Chen
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 451:734-7. 2008
    ..Together, these processes probably facilitate heterochromatin maintenance through successive cell divisions...
  99. pmc Erasing the methyl mark: histone demethylases at the center of cellular differentiation and disease
    Paul A C Cloos
    Biotech Research and Innovation Centre, University of Copenhagen, DK 2200 Copenhagen, Denmark
    Genes Dev 22:1115-40. 2008
    The enzymes catalyzing lysine and arginine methylation of histones are essential for maintaining transcriptional programs and determining cell fate and identity. Until recently, histone methylation was regarded irreversible...
  100. pmc Distinct DNA methylation patterns characterize differentiated human embryonic stem cells and developing human fetal liver
    Alayne L Brunner
    Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA
    Genome Res 19:1044-56. 2009
    To investigate the role of DNA methylation during human development, we developed Methyl-seq, a method that assays DNA methylation at more than 90,000 regions throughout the genome...
  101. pmc Effects of nickel, chromate, and arsenite on histone 3 lysine methylation
    Xue Zhou
    Nelson Institute of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, NY 10987, USA
    Toxicol Appl Pharmacol 236:78-84. 2009
    ..may be attributable in part, to activation and/or repression of gene expression induced by changes in the DNA methylation status and histone tail post-translational modifications...

Research Grants126 found, 100 shown here

  1. Variation in M. tuberculosis in response to host selection
    Sarah Fortune; Fiscal Year: 2009
    ..In these studies, we expect to provide fundamental insights into the mechanisms and targets of diversifying immune selection in M. tuberculosis. ..
  2. High Throughput Genotyping and DNA Sequencing for Studying the Genetic Contributi
    Sonia Dua; Fiscal Year: 2013
    ..3) A [unreadable]genotyping[unreadable] platform capable of measuring DNA methylation in human samples using arrays that cover 10,000 to 250,000 potential sites of methylation in the human genome...
  3. Yun Fai Chris Lau; Fiscal Year: 2014
    ..oncogenesis in the TSPY-LADY bi-transgenic mice, in terms of tumor pathogenesis, transgene expression, DNA methylation status of the TSPY promoter and exon 1, and identification of transcription factors and repressors affected by ..
  4. Tibor T Glant; Fiscal Year: 2016
    ..diseases, and St6galnac6 expression appears to play a key role in early inflammatory reactions, in Aim 3 we will focus on how the altered methylation of the St6galnac6 gene affects the development of inflammation of in PGISpA.
  5. Carole R Mendelson; Fiscal Year: 2016
    ..promoter of coactivators and corepressors eliciting modifications in histone phosphorylation, acetylation and methylation that likely cause profound changes in chromatin structure...
  6. Discovery of Small Molecule MBT Domain Antagonists
    STEPHEN VERNON FRYE; Fiscal Year: 2010
    ..The most important modifications of histones and DNA include: histone lysine and arginine methylation;lysine acetylation;DNA cytosine methylation;and histone sumoylation, ubiquitination, ADP-ribosylation and ..
  7. Jian Min Yuan; Fiscal Year: 2014
    ..studies within the two cohorts to elucidate the role of environmental exposure, genetic variants and DNA methylation in the development of lung or liver cancer...
  8. Neil W Kowall; Fiscal Year: 2014
    ..of neuroprotective polyamines that are potential substrates for LSD1, a key enzyme regulating protein methylation that shares considerable homology with FAD-dependent polyamine oxidases...
  9. Lung Lysyl Oxidase Regulation by Metal Ion Homeostasis
    Wande Li; Fiscal Year: 2013
    ..by metal and redox-sensitive transcription factors and their cognate cis-elements, and determining LO promoter methylation to provide evidence for Cd epigenetic damage to LO DNA;3) to identify mechanisms for Cd silencing of the LO ..
  10. MOLECULAR BASIS OF INSULIN RESISTANCE
    Maureen J Charron; Fiscal Year: 2010
    ..We propose that increased susceptibility to the development of Metabolic Syndrome is mediated by altered methylation of genes that regulate glucose and lipid utilization in liver...
  11. Translational Machinery in Stress Signaling and Tumor Suppression
    THOMAS J contact BEGLEY; Fiscal Year: 2010
    ..In colon carcinoma cells TRM9 down-regulation appears to be linked to promoter sequence methylation. Our results suggest that in HEp3 carcinoma cells TRM9 is epigenetically silenced...
  12. DNA methylation of extracellular superoxide dismutase in pulmonary hypertension
    EVA NOZIK GRAYCK; Fiscal Year: 2012
    ..It is now recognized that gene expression can be regulated by epigenetic modifications including cytosine methylation within the promoter region, specifically cytosines adjacent to guanosine nucleotides (CpG islands)...
  13. Rajvir Dahiya; Fiscal Year: 2015
    ..and then analyze hypomethylation of CpG Islands in putative promoter regions of miRNAs using sodium bisulfite methylation techniques and direct DNA sequencing...
  14. Epigenetics of Sex Differences in Brain and Behavior
    BRIDGET NUGENT; Fiscal Year: 2012
    ..DNA methylation is the epigenetic process by which a DNA methyltransferase (DNMT) enzyme covalently modifies cytosine ..
  15. Schahram Akbarian; Fiscal Year: 2014
    ..including (a) multiple lines of genetically engineered mice to target the regulation of histone (H3-lysine 9) methylation in neuronal chromatin, (b) procedures to isolate neuronal chromatin from brain tissue, (c) chromatin ..
  16. Hepatic Steatosis Modulation by Apo B Gene Transcription
    JANET DEHOFF SPARKS; Fiscal Year: 2010
    ..Aim 3 examines the role of DNA methylation in regulating apob gene expression as a potential mechanism of betaine action by assessment of global and ..
  17. Mitzi Nagarkatti; Fiscal Year: 2015
    ..interactions between CD44 with HA or OPN trigger epigenetic regulation by transcription factor gene promoter methylation and/or expression of miRs that lead to a switch in T differentiation from Th1/T17 cells to Th2/Tregs...
  18. Regulation of Airway Mucin Gene Expression by Epigenetic Mechanism
    Reen Wu; Fiscal Year: 2013
    ..in various human airway cell lines and primary normal bronchial epithelial (NHBE) cells are affected by the methylation status of the CpG islands...
  19. Alvaro Puga; Fiscal Year: 2015
    ..DNMT1 complexes to promoter chromatin and inhibiting phosphorylation, acetylation and methylation epigenetic marks established by BaP-induced gene transactivation in histones H3 and H4...
  20. John W Wiley; Fiscal Year: 2016
    ..refers to stable and/or heritable changes in gene function without changes in the DNA sequence via DNA methylation, histone modification and chromatin remodeling...
  21. David P Gavin; Fiscal Year: 2016
    ..DNA methylation is one component of epigenetic gene regulation...
  22. Chemical modulators of Tet-family proteins
    Anjana Rao; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): The methylation status of DNA influences many biological processes during mammalian development, including retrotransposon silencing, X-inactivation and the asymmetric expression of parentally ..
  23. CPG METHYLATION AND MUTATION
    Gerd P Pfeifer; Fiscal Year: 2013
    ..This application will focus on mechanistic studies that will investigate the molecular pathways leading to DNA methylation changes in tumors with emphasis on DNA hypermethylation...
  24. Bioflavonoid Effects on EGF Signaling and Cell Cycle Pathways in Prostate Cancer
    BARRY MATTHEW MARKAVERICH; Fiscal Year: 2013
    ..dimers) suggests that ligand occupancy of this site modulates post-translational modifications (acetylation, methylation, phosphorylation, etc.) of histones H3 or H4 that regulate gene transcription...
  25. TIMOTHY L SITA; Fiscal Year: 2016
    ..TMZ cytotoxicity is mediated primarily through methylation of the O6-position of guanine...
  26. Rafi Ahmed; Fiscal Year: 2016
    ..Examination of the epigenetic DMA methylation marks during the progression of the T cell response, as well as between CD8 T cells responding to acute versus ..
  27. Howard B Lieberman; Fiscal Year: 2016
    ..and DNMT3b are highly expressed in certain prostate cancer cell lines because of chromatin structure, promoter methylation abnormalities or mutation, and bind more tightly to Rad9 only in DU145, AVLA31 and AVLA41 versus other cancer ..
  28. Rebecca A Simmons; Fiscal Year: 2016
    ..In addition to testing the expression and DNA methylation of imprinted genes, we will assay genome-wide DNA methylation changes and key histone modifications in F1-F3 ..
  29. Genistein and Prostate Cancer
    Rajvir Dahiya; Fiscal Year: 2010
    ..hypothesize that genistein inhibits prostate cancer growth through modulation of epigenetic events, such as DNA methylation and histone acetylation...
  30. Epigenetic effects of developmental endocrine disruptor exposure in the ovary
    Mehmet Uzumcu; Fiscal Year: 2010
    ..Epigenetic mechanisms (e.g., DNA methylation) may play a role as a link between environmental EDC exposures during development and the adult diseases...
  31. Subhash C Pandey; Fiscal Year: 2016
    ..epigenetic regulatory mechanism which has been shown to play a role in the regulation of gene expression is DNA methylation. DNA methyltransferases (DNMT) catalyze DNA methylation allowing for the binding of methyl-CpG-binding proteins ..
  32. Disease chronicity in juvenile dermatomyositis (JDM): Epigenetic clues
    Lauren M Pachman; Fiscal Year: 2013
    ..The purpose of this study is to identify epigenetic mechanisms - differences in global methylation and miRNA expression - critical in dissecting the impact of chronic inflammation and gender on JDM ..
  33. Transplacental Arsenic Induced Hepatic Dysfunction and Vascular Disease
    J Christopher States; Fiscal Year: 2010
    ..Transplacental arsenic exposure can disrupt liver DNA methylation patterns and thus alter liver gene expression...
  34. Effects of In Utero and Transgenerational Exposure of Avy Mice to Bisphenol A
    CHERYL SUSAN ROSENFELD; Fiscal Year: 2010
    ..IAP) in their agouti gene, exposure to a maternal diet-enriched in genistein or methyl- donors results in methylation of the IAP site and a graded shift from yellow coat color to pseudoagouti offspring...
  35. Folate and S-adenosyimethionine in Methyl Group and One-carbon Metabolism
    Conrad Wagner; Fiscal Year: 2012
    Regulation of methylation is intimately associated with folate metabolism...
  36. MECHANISMS OF EUKARYOTIC TRANSCRIPTIONAL REGULATION
    Michael R Green; Fiscal Year: 2013
    ..activity of many genes is controlled by epigenetic mechanisms that involve histone modifications and DNA methylation. The detailed mechanisms and pathways by which epigenetic repression is established and maintained on specific ..
  37. Mechanism of Age-Related Change in Estrogen Receptor Beta Expression in Rat Brain
    JENNE MITCHELL WESTBERRY; Fiscal Year: 2012
    ..mechanism of ER2 gene regulation is through epigenetic modification of C-G rich regions of the ER1 promoter by methylation or acetylation of histones associated with the gene...
  38. Improving Epigenetic-based Cell Reprogramming with Proteasome Inhibition
    Jaroslaw Staszkiewicz; Fiscal Year: 2011
    ..1) * altering the single carbon metabolism pathway and limiting methyl donors in culture medium to modulate * methylation capacity;2) knocking down gene expression of repressive epigenetic regulatory proteins (ie...
  39. Folate Status, Genomic Uracil, and the Balance of Base Excision Repair Activity
    Michael D Wyatt; Fiscal Year: 2011
    ..are important sources of one-carbon units for de novo synthesis of nucleotides and are necessary for enzymatic methylation of CpG sequences for gene silencing...
  40. Alfonso Bellacosa; Fiscal Year: 2014
    ..MED1 and TDG, maintain genomic and epigenomic stability at CpG sites, by preventing both mutations and altered methylation patterns...
  41. DHANANJAY MADHUKAR VAIDYA; Fiscal Year: 2015
    ..Urine arsenic concentrations and methylation excretion patterns remained constant over a 10-year follow-up...
  42. Role of ALDH7A1 in Ethanol-Induced Oxidative Damage
    CHAD NICHOLAS BROCKER; Fiscal Year: 2011
    ..SAM is the principle biological methyl donor and required for protein, phospholipid and DNA methylation. In the liver, chronic ethanol consumption both dramatically elevates LPO-derived aldehyde production and ..
  43. CRAIG STUART PIKAARD; Fiscal Year: 2014
    ..These novel RNA polymerases play non- redundant roles in RNA-directed DNA methylation, silencing of transposable elements, large-scale heterochromatin organization, long-distance spreading of ..
  44. Epigenetic downregulation of the antibody response and inhibition of autoimmunity
    Paolo Casali; Fiscal Year: 2013
    ..of the antibody response and inhibition of autoimmunity Epigenetic marks (including histone modifications, DNA methylation and microRNAs) "interact" with genetic programs to regulate B cell functions, such as class switch DNA ..
  45. Analysis of epigenetic regulation in early mammalian embryos via RNA interference
    Charles R Long; Fiscal Year: 2010
    ..Two major mechanisms appear to be responsible for establishing and maintaining the epigenome, DNA methylation and histone modifications...
  46. Interrelationships among nutrients involved in one-carbon metabolism in Banglades
    MEGAN NINA HALL; Fiscal Year: 2011
    ..the current area of research, investigating the influence of nutrients involved in one-carbon metabolism on the methylation of both arsenic (As) and genomic DNA. Specifically, the roles of two nutrients, choline and betaine, i.e...
  47. Exploring persistent epigenetic changes after prenatal famine exposure in humans
    L H Lumey; Fiscal Year: 2010
    ..DNA methylation, one type of epigenetic change, may play an important role in disease causation by altering the proteins that ..
  48. B Cell Development in Aging
    John C Cambier; Fiscal Year: 2010
    ..Chromatin accessibility is regulated by histone modification (e.g. acetylation, methylation, phosphorylation, etc.) and methylation of DNA CpG dinucleotides...
  49. Biomarkers to Assess Selenium Chemoprevention for NSCLC
    Steven A Belinsky; Fiscal Year: 2012
    ..Our studies have identified genes inactivated by aberrant cytosine-guanosine (CpG) island methylation as candidate biomarkers for early detection of lung cancer...
  50. Leena A Hilakivi-Clarke; Fiscal Year: 2015
    ..In addition, we will investigate whether these transgenerational effects involve changes in DNA methylation. Our preliminary analysis performed using massively parallel sequencing identified 144 "named" genes which were ..
  51. SATB1-dependent epigenetic regulation of leukemia initiating cells
    Britta Will; Fiscal Year: 2011
    ..utilize global chromatin immunoprecipitation analysis (ChIP-seq) in combination with gene expression and DNA methylation pattern analysis...
  52. Jianhua Shao; Fiscal Year: 2016
    ..A methylation region was identified in the promoter of C/EBP2 gene...
  53. Evaluating the role of histone H3K79 methylation states in DNA damage repair in S
    Jeffrey S Thompson; Fiscal Year: 2010
    ..We and others have previously found that a specific histone modification, methylation of lysine-79 in histone H3 (H3K79), is important for the response to DNA damage caused by UV radiation...
  54. Rajvir Dahiya; Fiscal Year: 2014
    ..Human prostate cancer tissues will be used for analysis of methylation of miRNAs...
  55. Vitamin B6 Effects on One-Carbon Metabolism
    Jesse F Gregory; Fiscal Year: 2013
    ..as the synthesis of nucleotides and glutathione, metabolism of most amino acids, including sulfur amino acids, methylation processes, and gluconeogenesis. Low B6 status potentially can interfere with many of these processes...
  56. SHELLY CHI LOO LU; Fiscal Year: 2016
    ..Furthermore, our preliminary data show SAMe and MTA modulate histone methylation and acetylation to affect gene expression...
  57. Tracie R Baker; Fiscal Year: 2016
    ..from each generation and will use both gene- specific and global techniques to examine alterations in DNA methylation and chromatin structure induced by TCDD exposure and transmitted into F1 and F2 fish...
  58. Arsenic, Histone Modification, and Transcription
    LYNN SHELDON; Fiscal Year: 2009
    ..GR-mediated histone modifications, acetylation and methylation, will be identified at the mouse mammary tumor virus (MMTV) promoter in the presence and absence of sodium ..
  59. JENNIFER ANNE RINKER; Fiscal Year: 2014
    ..These posttranslational changes include: acetylation, phosphorylation, and methylation of histone amino acid residues...
  60. CONTROL OF EUCARYOTIC FUNCTION BY METHYLATION
    Steven G Clarke; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): We will investigate how physiological functions are controlled by methylation reactions, focusing on the enzymes that catalyze the modification of protein arginine, lysine, and isoaspartyl residues...
  61. Kip E Guja; Fiscal Year: 2014
    ..Both the stem-loop structure and methylation events are conserved in the human 12S mitochondrial rRNA...
  62. David M Lyons; Fiscal Year: 2014
    ..e., increased DNA methylation) and thereby increases anticipatory impulsivity. Specifically, we address the following three aims. Aim 1...
  63. Mitzi Nagarkatti; Fiscal Year: 2015
    ..Th1/Th17cells and induction of T regs/Th2 cells via epigenetic regulatory mechanisms such as DNA methylation and miRNA dysregulation that block neuroinflammation...
  64. ANDREW GEORGE MUNTEAN; Fiscal Year: 2014
    ..with RNA polymerase II and promotes histone H2B ubiquitination (a prerequisite for H3 lysine 4 and 79 methylation). The MLL-PAFc interaction is essential for leukemogenesis by MLL fusion proteins...
  65. Amit Berson; Fiscal Year: 2015
    DESCRIPTION (provided by applicant): Epigenetic chromatin modifications such as histone methylation and acetylation are emerging as prominent regulators of human development, disease and aging...
  66. Regulation of ENaC Transcription
    Bruce C Kone; Fiscal Year: 2013
    ..at the ??ENaC promoter, interactions with novel factors, and changes in histone modifications and promoter methylation. Studies in BAC transgenic mice will test whether the candidate AF9 binding site is critical for the responses ..
  67. Paolo Rinaudo; Fiscal Year: 2014
    ..The aberrant txnip gene expression is associated with altered expression of several genes that control DNA methylation, suggesting an epigenetic regulation...
  68. Herpesvirus protein kinases: Substrate recognition and pathway targeting.
    S Diane Hayward; Fiscal Year: 2013
    ..whose expression is regulated by BGLF4/TIP60 mediated chromatin remodeling by interrogating genome wide CpG methylation of Akata B cell DNA after doxycycline induction of BGLF4 and control kinase dead BGLF4...
  69. Jean Pierre J Issa; Fiscal Year: 2016
    DESCRIPTION (provided by applicant): In normal cells, DNA methylation is rare in promoter-associated CpG islands but important to X-inactivation, genomic imprinting and repression of repetitive elements...
  70. Farida Sohrabji; Fiscal Year: 2015
    ..In Specific Aim 2 we will determine age-related changes in DNA and histone methlyation patterns. Methylation patterns of specific leucines associated with activation (H3K4me3 and H3K9ac) or repression (H3K9me3 and ..
  71. Karam F A Soliman; Fiscal Year: 2016
    ..services in gene cloning, constructs for knock-downs, -outs and -ins, stable transfectants, DNA sequencing, methylation, histone modifications;chromatin biochemistry, RNA analysis, sequencing and synthesis, and microarray services ..
  72. Margareta Pisarska; Fiscal Year: 2015
    ..Specifically, we propose to quantify and compare gene expression and CpG DNA methylation, as well as genome-wide SNP and copy number variation (CNV) profiles and exome-wide non-synonymous SNP profiles,..
  73. Michael R Stallcup; Fiscal Year: 2016
    ..involves recruitment of corepressor complexes which often include enzymes that deacetylate and make repressive methylation marks on histones...
  74. Glucocorticoid Receptor and Lipid Homeostasis
    Jen Chywan Wang; Fiscal Year: 2013
    ..In Aim 1, we will analyze the effects of glucocorticoids on chromatin structure, and histone acetylation and methylation status of FIAF gene...
  75. Samson T Jacob; Fiscal Year: 2016
    ..In the present study we will examine whether its promoter methylation can be used as biomarker for liver cancer of specific etiology and its function in liver...
  76. Jennifer A Woyach; Fiscal Year: 2016
    ..trial to evaluate baseline and dynamic markers to determine 1) Whether baseline cytogenetic markers, Zap-70 methylation, IgVH mutational status, or select DNA mutations predict outcomes or time to response;2) Whether eradication of ..
  77. Fengchun Ye; Fiscal Year: 2014
    ..the polycomb repressive complex (PRC2), leading to histone hyperacetylation and reduction of repressive histone methylation simultaneously...
  78. Prenatal Cannabis Exposure and Epigenetic Mechanisms Underlying Vulnerability to
    CLAUDIA VARGAS MORRIS; Fiscal Year: 2013
    ..Preliminary data indicate that the Drd2 gene contains abnormal histone H3 methylation marks in adult rats that were exposed to THC prenatally...
  79. Charles M Perou; Fiscal Year: 2016
    ..tools to study gene expression (our role), tumor genomic DNA copy number alterations, SNP genotypes, DNA methylation and gene mutational analyses...
  80. Charles V Vorhees; Fiscal Year: 2015
    ..heritable transgenerational phenotypic changes in CNS and cardiac physiology associated with changes in DNA methylation patterns responsible for altered chromatin conformation and subsequent dysregulation of gene expression, ..
  81. Ivo Teneng; Fiscal Year: 2014
    ..While it is known that DNMTs cooperate in de novo methylation and gene silencing, very little is known about targets for DNMT3a and DNMT3b or their impact on the development ..
  82. RNA Modifications as Biomarkers of Environmental Stress and Inflammation
    Thomas J Begley; Fiscal Year: 2013
    ..We have recently demonstrated that enzyme-catalyzed methylation of a modified uridine (mcm5U) in tRNA plays a role in codon- specific translational control after DNA damage, ..
  83. Joseph S Takahashi; Fiscal Year: 2016
    ..and RNA polymerase II recruitment and initiation as well as chromatin modifications involving histone methylation and acetylation...
  84. Margaret A Goodell; Fiscal Year: 2016
    DESCRIPTION (provided by applicant): DNA methylation is an important epigenetic modification that serves to protect the genome from propagating mutations, and to regulate gene expression...
  85. Herbert M Lachman; Fiscal Year: 2016
    ..allele-biased expression in differentiating human neurons will be explored by carrying out genome-wide DNA methylation and chromatin immunoprecipitation studies...
  86. Suresh Cuddapah; Fiscal Year: 2016
    ..In addition, global levels of histone modifications and DNA methylation and hydroxymethylation will be assessed to confirm the identity of oxidative demethylases that are inhibited...
  87. Rachel L Miller; Fiscal Year: 2016
    ..Moreover, we have made important strides in the understanding of epigenetic changes (eg. DNA methylation) that may occur in association with such exposures...
  88. JUAN CARLOS CELEDON; Fiscal Year: 2016
    ..The best studied epigenetic mechanism is DNA methylation, the covalent addition of a methyl group to a cytosine residue occurring mostly in a CpG site (i.e...
  89. Andrea Baccarelli; Fiscal Year: 2015
    ..aging by means of a panel of complementary markers of oxidative damage, heteroplasmy and mtDNA abundance;and 2) methylation of nuclear DNA (nDNA) genes coding for mitochondrial proteins (for which we will have the unique opportunity to ..
  90. Michelle L Olsen; Fiscal Year: 2016
    ..of these proteins a direct result of epigenetic modulation of gene transcription? 3) Can manipulation of DNA methylation using FDA-approved drugs reverse the loss of Kir4...
  91. Kristi L Watterberg; Fiscal Year: 2016
    ..of neonatal illness and postnatal growth trajectory;(3) these measures will correlate positively with DNA methylation at sites regulating corticosteroid expression...
  92. Rugang Zhang; Fiscal Year: 2016
    ..of zeste homolog 2 (EZH2) is a histone methyltransferase that silences gene expression by generating tri-methylation on lysine 27 residue of histone H3 (H3K27Me3)...
  93. Nancy Bettina Spinner; Fiscal Year: 2015
    ..Epigenetic modifications, such as DNA methylation, function as stable gene silencing mechanisms that repress gene expression in differentiated cells...
  94. Kelly L Gorres; Fiscal Year: 2014
    ..This aim will identify the effects of SCFAs on histone modifications, such as lysine methylation, at the viral transactivator gene promoters and if those modifications influence EBV reactivation...
  95. Biomarkers for Epigenetic Therapy of Lung Cancer
    Mathewos Tessema; Fiscal Year: 2013
    ..Epigenetic therapy can simultaneously target hundreds of genes aberrantly silenced by methylation;however, the number and type of methylated genes within a tumor could influence its effectiveness...
  96. Alessandro Guidotti; Fiscal Year: 2015
    ..The research we are proposing would be an addendum to this larger study. DNA methylation and demethylation are key epigenetic components involved in orchestrating transcription of specific genes in ..
  97. Jay S Schneider; Fiscal Year: 2016
    ..later life cognitive functioning is through epigenetic modifications and specifically, through modulating DNA methylation. Recent studies show that DNA methylation in the adult brain is highly dynamic and that the dynamic regulation ..
  98. Epigenetic regulation of programmed genome instability in O. trifallax
    JOHN RUSSELL BRACHT; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Cytosine methylation of DNA is a repressive chromatin mark important for silencing genes during mammalian development, and cancer cells frequently silence tumor suppressor genes by aberrantly ..
  99. Mary Szatkowski Ozers; Fiscal Year: 2015
    ..the common cancer- related post-translational modifications of phosphorylation, sumoylation, and arginine methylation. Lysates prepared from cancer cells will be applied to the SNAP-Tide array to evaluate differences in their ..
  100. Robert W Sobol; Fiscal Year: 2014
    ..Specifically, we wil characterize a novel regulatory mechanism of Polss (lysine di-methylation) that can be exploited to inhibit Polss and enhance alkylating agent-induced cell death by enhancing the ..