membrane fusion

Summary

Summary: The adherence and merging of cell membranes, intracellular membranes, or artificial membranes to each other or to viruses, parasites, or interstitial particles through a variety of chemical and physical processes.

Top Publications

  1. pmc Membrane fusion: grappling with SNARE and SM proteins
    Thomas C Sudhof
    Department of Cellular and Molecular Physiology, Stanford University, Palo Alto, CA 94304, USA
    Science 323:474-7. 2009
  2. pmc Structure of dengue virus: implications for flavivirus organization, maturation, and fusion
    Richard J Kuhn
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Cell 108:717-25. 2002
  3. ncbi The membrane fusion enigma: SNAREs, Sec1/Munc18 proteins, and their accomplices--guilty as charged?
    Josep Rizo
    Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Annu Rev Cell Dev Biol 28:279-308. 2012
  4. ncbi SNAP receptors implicated in vesicle targeting and fusion
    T Sollner
    Rockefeller Research Laboratory, Memorial Sloan Kettering Cancer Center, New York, New York 10021
    Nature 362:318-24. 1993
  5. ncbi A structural perspective of the flavivirus life cycle
    Suchetana Mukhopadhyay
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, Indiana 47907 2054, USA
    Nat Rev Microbiol 3:13-22. 2005
  6. pmc OPA1 requires mitofusin 1 to promote mitochondrial fusion
    Sara Cipolat
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Via Orus 2, I 35129 Padua, Italy
    Proc Natl Acad Sci U S A 101:15927-32. 2004
  7. ncbi Lysosomes: fusion and function
    J Paul Luzio
    Cambridge Institute for Medical Research, Cambridge, CB2 0XY, UK
    Nat Rev Mol Cell Biol 8:622-32. 2007
  8. ncbi Mitochondrial fusion protects against neurodegeneration in the cerebellum
    Hsiuchen Chen
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Cell 130:548-62. 2007
  9. pmc Mitochondrial fragmentation in neurodegeneration
    Andrew B Knott
    Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 4000 Central Florida Boulevard, Orlando, Florida 32816, USA
    Nat Rev Neurosci 9:505-18. 2008
  10. pmc Characterization of the early events in dengue virus cell entry by biochemical assays and single-virus tracking
    Hilde M van der Schaar
    Department of Medical Microbiology, University Medical Center Groningen, University of Groningen, Ant Deusinglaan 1, 9700 RB Groningen, The Netherlands
    J Virol 81:12019-28. 2007

Research Grants

  1. Sphingolipid Metabolism in Drosophila Development
    Julie D Saba; Fiscal Year: 2013
  2. Human cytomegalovirus entry into epithelial and endothelial cells
    David C Johnson; Fiscal Year: 2013
  3. Disease-related defects in dendritic cell processing of bacterial antigens
    Michael S Marks; Fiscal Year: 2012
  4. Cellular Components Involved in Japanese Encephalitis Virus Entry
    Young Min Lee; Fiscal Year: 2013
  5. Mechanistic Studies of HCV E2
    Joseph Marcotrigiano; Fiscal Year: 2013
  6. Phosphoprotein CDP138 regulates glucose metabolism
    ZHEN YUE JIANG; Fiscal Year: 2013
  7. 2011 Cell-Cell Fusion Gordon Research Conference and Seminar
    NANCY RYAN GRAY; Fiscal Year: 2011
  8. Cardiac function and PIP2
    Donald W Hilgemann; Fiscal Year: 2013
  9. MEMBRANE FUSION IN RETINAL ROD OUTER SEGMENTS
    KATHLEEN BOESZEBATTAGLIA; Fiscal Year: 2012
  10. Cloning and Analysis of a Gamete Fusion Gene
    CHARLENE FOREST; Fiscal Year: 2009

Detail Information

Publications350 found, 100 shown here

  1. pmc Membrane fusion: grappling with SNARE and SM proteins
    Thomas C Sudhof
    Department of Cellular and Molecular Physiology, Stanford University, Palo Alto, CA 94304, USA
    Science 323:474-7. 2009
    The two universally required components of the intracellular membrane fusion machinery, SNARE and SM (Sec1/Munc18-like) proteins, play complementary roles in fusion...
  2. pmc Structure of dengue virus: implications for flavivirus organization, maturation, and fusion
    Richard J Kuhn
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Cell 108:717-25. 2002
    ..The structure suggests that flaviviruses, and by analogy also alphaviruses, employ a fusion mechanism in which the distal beta barrels of domain II of the glycoprotein E are inserted into the cellular membrane...
  3. ncbi The membrane fusion enigma: SNAREs, Sec1/Munc18 proteins, and their accomplices--guilty as charged?
    Josep Rizo
    Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Annu Rev Cell Dev Biol 28:279-308. 2012
    Neurotransmitter release is governed by proteins that have homo-logs in most types of intracellular membrane fusion, including the Sec1/Munc18 protein Munc18-1 and the SNARE proteins syntaxin-1, synaptobrevin/VAMP, and SNAP-25...
  4. ncbi SNAP receptors implicated in vesicle targeting and fusion
    T Sollner
    Rockefeller Research Laboratory, Memorial Sloan Kettering Cancer Center, New York, New York 10021
    Nature 362:318-24. 1993
    ..NSF) and the soluble NSF attachment proteins (SNAPs) appear to be essential components of the intracellular membrane fusion apparatus...
  5. ncbi A structural perspective of the flavivirus life cycle
    Suchetana Mukhopadhyay
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, Indiana 47907 2054, USA
    Nat Rev Microbiol 3:13-22. 2005
    ..This review focuses mainly on structural studies of dengue virus...
  6. pmc OPA1 requires mitofusin 1 to promote mitochondrial fusion
    Sara Cipolat
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Via Orus 2, I 35129 Padua, Italy
    Proc Natl Acad Sci U S A 101:15927-32. 2004
    ..Our data show that OPA1 functionally requires mitofusin 1 to regulate mitochondrial fusion and reveal a specific functional difference between mitofusin 1 and 2...
  7. ncbi Lysosomes: fusion and function
    J Paul Luzio
    Cambridge Institute for Medical Research, Cambridge, CB2 0XY, UK
    Nat Rev Mol Cell Biol 8:622-32. 2007
    ..However, the function of lysosomes is not restricted to protein degradation: they also fuse with the plasma membrane during cell injury, as well as having more specialized secretory functions in some cell types...
  8. ncbi Mitochondrial fusion protects against neurodegeneration in the cerebellum
    Hsiuchen Chen
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Cell 130:548-62. 2007
    ....
  9. pmc Mitochondrial fragmentation in neurodegeneration
    Andrew B Knott
    Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 4000 Central Florida Boulevard, Orlando, Florida 32816, USA
    Nat Rev Neurosci 9:505-18. 2008
    ..This results in impaired bioenergetics and mitochondrial migration, and can trigger neurodegeneration. These findings suggest potential new treatment avenues for neurodegenerative diseases...
  10. pmc Characterization of the early events in dengue virus cell entry by biochemical assays and single-virus tracking
    Hilde M van der Schaar
    Department of Medical Microbiology, University Medical Center Groningen, University of Groningen, Ant Deusinglaan 1, 9700 RB Groningen, The Netherlands
    J Virol 81:12019-28. 2007
    ..In order to explain this high ratio, we investigated the receptor binding and membrane fusion characteristics of single DENV particles in living cells using real-time fluorescence microscopy...
  11. ncbi Dynamics of mitochondrial morphology in healthy cells and during apoptosis
    M Karbowski
    The Biochemistry Section, SNB, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Death Differ 10:870-80. 2003
    ..In this review the recent progress in understanding the mechanisms governing mitochondrial morphology and the latest advances connecting the regulation of mitochondrial morphology with programmed cell death are discussed...
  12. pmc At the junction of SNARE and SM protein function
    Chavela M Carr
    Department of Biochemistry and Biophysics, Texas A and M University, 300 Olsen Blvd, College Station, TX 77843 2128, USA
    Curr Opin Cell Biol 22:488-95. 2010
    ..is a picture of SM proteins acting not merely as SNARE regulators, but also as central components of the membrane fusion apparatus...
  13. ncbi Multisubunit tethering complexes and their role in membrane fusion
    Cornelia Bröcker
    University of Osnabruck, Department of Biology Chemistry, Barbarastrasse 13, 49076 Osnabruck, Germany
    Curr Biol 20:R943-52. 2010
    ....
  14. ncbi Synaptic vesicle fusion complex contains unc-18 homologue bound to syntaxin
    Y Hata
    Howard Hughes Medical Institute, University of Texas Southwestern Medical School, Dallas 75235
    Nature 366:347-51. 1993
    ..for membrane receptors for N-ethylmaleimide-sensitive fusion protein (NSF), which has an important role in membrane fusion, revealed an ATP-dependent interaction of the same three synaptic proteins with NSF and its soluble ..
  15. ncbi SNAREs--engines for membrane fusion
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg, 37077 Gottingen, Germany
    Nat Rev Mol Cell Biol 7:631-43. 2006
    ..proteins in the late 1980s, SNAREs have been recognized as key components of protein complexes that drive membrane fusion. Despite considerable sequence divergence among SNARE proteins, their mechanism seems to be conserved and is ..
  16. ncbi Structure of the dengue virus envelope protein after membrane fusion
    Yorgo Modis
    Howard Hughes Medical Institute, Children s Hospital and Harvard Medical School, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 427:313-9. 2004
    ..Specific features of the folded-back structure suggest strategies for inhibiting flavivirus entry...
  17. ncbi OPA1 controls apoptotic cristae remodeling independently from mitochondrial fusion
    Christian Frezza
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Padova, Italy
    Cell 126:177-89. 2006
    ..The proapoptotic BCL-2 family member BID, which widens cristae junctions, also disrupts OPA1 oligomers. Thus, OPA1 has genetically and molecularly distinct functions in mitochondrial fusion and in cristae remodeling during apoptosis...
  18. pmc Structural changes of envelope proteins during alphavirus fusion
    Long Li
    Department of Biological Sciences, Purdue University, 915 W State Street, West Lafayette, Indiana 47907 2054, USA
    Nature 468:705-8. 2010
    ..The E1 glycoprotein has a hydrophobic fusion loop at one end and is responsible for membrane fusion. The E2 protein is responsible for receptor binding and protects the fusion loop at neutral pH...
  19. pmc Mitofusins and OPA1 mediate sequential steps in mitochondrial membrane fusion
    Zhiyin Song
    Howard Hughes Medical Institute and Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Mol Biol Cell 20:3525-32. 2009
    ..In yeast, the OPA1 ortholog Mgm1 is required for inner membrane fusion in vitro; nevertheless, yeast lacking Mgm1 show neither outer nor inner membrane fusion in vivo, because of ..
  20. ncbi Munc13-4 is essential for cytolytic granules fusion and is mutated in a form of familial hemophagocytic lymphohistiocytosis (FHL3)
    Jerome Feldmann
    INSERM U429, Hopital Necker Enfants Malades, 75743 Paris, France
    Cell 115:461-73. 2003
    ..Expressed tagged hMunc13-4 localizes with cytotoxic granules at the immunological synapse. HMunc13-4 is therefore essential for the priming step of cytolytic granules secretion preceding vesicle membrane fusion.
  21. ncbi The synaptic vesicle cycle
    Thomas C Sudhof
    Center for Basic Neuroscience, Department of Molecular Genetics, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9111, USA Thomas
    Annu Rev Neurosci 27:509-47. 2004
    ..The present review attempts to relate these molecular data with physiological results in an emerging view of nerve terminals as macromolecular machines...
  22. ncbi Secretory lysosomes
    Emma J Blott
    Sir William Dunn School of Pathology, Oxford University, South Parks Rd, Oxford OX1 3RE, UK
    Nat Rev Mol Cell Biol 3:122-31. 2002
    ..The importance of these organelles is highlighted by several genetic diseases, in which immune function and pigmentation--two processes that normally involve secretory lysosomes--are impaired...
  23. pmc Herpesvirus entry: an update
    Patricia G Spear
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Virol 77:10179-85. 2003
  24. ncbi Mitofusin-1 protein is a generally expressed mediator of mitochondrial fusion in mammalian cells
    Ansgar Santel
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Cell Sci 116:2763-74. 2003
    ..Thus, Mfn1 appears to be a key player in mediating mitochondrial fusion and morphology in mammalian cells...
  25. ncbi LC3 and GATE-16 N termini mediate membrane fusion processes required for autophagosome biogenesis
    Hilla Weidberg
    Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel
    Dev Cell 20:444-54. 2011
    ..The biogenesis of autophagosomes and their delivery to the vacuole/lysosome depend on multiple membrane fusion events...
  26. ncbi Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion
    Hitoshi Nakatogawa
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    Cell 130:165-78. 2007
    ..Our results provide further insights into the mechanisms underlying the unique membrane dynamics of autophagy and also indicate the functional versatility of ubiquitin-like proteins...
  27. pmc Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p
    Charles Boyd
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Biol 167:889-901. 2004
    ..Assembly of the exocyst occurs when the first subset of subunits, delivered on vesicles, joins Sec3p and Exo70p on the plasma membrane. Exocyst assembly serves to both target and tether vesicles to sites of exocytosis...
  28. pmc Division versus fusion: Dnm1p and Fzo1p antagonistically regulate mitochondrial shape
    H Sesaki
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 147:699-706. 1999
    ..Our results thus suggest that mitochondrial shape is normally controlled by a balance between division and fusion which requires Dnm1p and Fzo1p, respectively...
  29. ncbi Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes
    J M Escola
    Department of Cell Biology, Utrecht University School of Medicine, AZU, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    J Biol Chem 273:20121-7. 1998
    ....
  30. ncbi Homotypic fusion of ER membranes requires the dynamin-like GTPase atlastin
    Genny Orso
    Eugenio Medea Scientific Institute, Conegliano 31015, Italy
    Nature 460:978-83. 2009
    ..Homotypic membrane fusion is required for ER biogenesis and maintenance, and has been shown to depend on GTP hydrolysis...
  31. ncbi Membrane fusion: five lipids, four SNAREs, three chaperones, two nucleotides, and a Rab, all dancing in a ring on yeast vacuoles
    William Wickner
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755 3844, USA
    Annu Rev Cell Dev Biol 26:115-36. 2010
    ....
  32. pmc Mitochondrial fusion in human cells is efficient, requires the inner membrane potential, and is mediated by mitofusins
    Frédéric Legros
    Institut National de la Santé et de la Recharche Médicale U523, Institut de Myologie, Groupe Hospitalier Pitie Salpetriere, 75651 Paris, France
    Mol Biol Cell 13:4343-54. 2002
    ..These results show that human mitochondria represent a single cellular compartment whose exchanges and interconnectivity are dynamically regulated by the balance between continuous fusion and fission reactions...
  33. pmc The crystal structure of a Munc13 C-terminal module exhibits a remarkable similarity to vesicle tethering factors
    Wei Li
    Department of Biochemistry, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
    Structure 19:1443-55. 2011
    ..We propose a model whereby the MUN-CD module is central for Munc13 function but full activity requires adjacent sequences...
  34. ncbi Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution
    R B Sutton
    The Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Nature 395:347-53. 1998
    ..These characteristics may be important for membrane fusion and for the binding of regulatory factors affecting neurotransmission.
  35. ncbi Structure of the parainfluenza virus 5 F protein in its metastable, prefusion conformation
    Hsien Sheng Yin
    Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois 60208 3500, USA
    Nature 439:38-44. 2006
    ..The positions and structural transitions of key parts of the fusion machinery, including the hydrophobic fusion peptide and two helical heptad repeat regions, clarify the mechanism of membrane fusion mediated by the F protein.
  36. ncbi Chemokine receptors as HIV-1 coreceptors: roles in viral entry, tropism, and disease
    E A Berger
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Immunol 17:657-700. 1999
    ..HIV-1, including the selective tropism of individual viral variants for different CD4+ target cells and the membrane fusion mechanism governing virus entry...
  37. ncbi Structures of vesicular stomatitis virus glycoprotein: membrane fusion revisited
    S Roche
    CNRS, UMR2472, INRA, UMR1157, IFR 115, Virologie Moléculaire et Structurale, Gif sur Yvette, France
    Cell Mol Life Sci 65:1716-28. 2008
    ..involved in receptor recognition at the host cell surface and then, after endocytosis of the virion, triggers membrane fusion via a low pH-induced structural rearrangement...
  38. ncbi Dynamin and its role in membrane fission
    J E Hinshaw
    Laboratory of Cell Biochemistry and Biology, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Cell Dev Biol 16:483-519. 2000
    ..The focus of this review is on the relationship between the GTPase and self-assembly properties of dynamin and its cellular function...
  39. pmc Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p
    Christopher Stroupe
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755 3844, USA
    EMBO J 25:1579-89. 2006
    Coupling of Rab GTPase activation and SNARE complex assembly during membrane fusion is poorly understood...
  40. pmc The dynamin-related GTPase Dnm1 regulates mitochondrial fission in yeast
    W Bleazard
    Department of Biology, University of Utah, Salt Lake City 84112, USA
    Nat Cell Biol 1:298-304. 1999
    ..These findings indicate that Dnm1 regulates mitochondrial fission, assembling on the cytoplasmic face of mitochondrial tubules at sites at which division will occur...
  41. ncbi Mitofusin 1 and 2 play distinct roles in mitochondrial fusion reactions via GTPase activity
    Naotada Ishihara
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    J Cell Sci 117:6535-46. 2004
    ..These findings indicate that the two Mfn proteins have distinct activities, and suggest that Mfn1 is mainly responsible for GTP-dependent membrane tethering...
  42. ncbi An emergency response team for membrane repair
    Paul L McNeil
    Department of Anatomy and Cellular Biology, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30921, USA
    Nat Rev Mol Cell Biol 6:499-505. 2005
    ....
  43. ncbi SNAREs contribute to the specificity of membrane fusion
    S J Scales
    Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, California 94305, USA
    Neuron 26:457-64. 2000
    Intracellular membrane fusion is mediated by the formation of a four-helix bundle comprised of SNARE proteins...
  44. ncbi Compartmental specificity of cellular membrane fusion encoded in SNARE proteins
    J A McNew
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 407:153-9. 2000
    ..compartments of the cytoplasm of eukaryotic cells, delivering their specific cargo to programmed locations by membrane fusion. The pairing of vesicle v-SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) ..
  45. pmc Ebola virus glycoprotein needs an additional trigger, beyond proteolytic priming for membrane fusion
    Shridhar Bale
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, USA
    PLoS Negl Trop Dis 5:e1395. 2011
    ..After entry into host endosomes, GP(1,2) is enzymatically cleaved by endosomal cathepsins B and L, a necessary step in infection. However, the functional effects of the cleavage on the glycoprotein are unknown...
  46. ncbi Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell surface projections regulates compound exocytosis in mast cells
    Z Guo
    Department of Cell Biology, University of Virgina Health Sciences Center, Charlottesville 22908, USA
    Cell 94:537-48. 1998
    ..While relocation is a prerequisite for secretion, it can occur without membrane fusion and will expedite a subsequent secretory response...
  47. ncbi Mitochondria: dynamic organelles in disease, aging, and development
    David C Chan
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Cell 125:1241-52. 2006
    ..Clearly, much about the basic biology of mitochondria remains to be understood. Here we discuss recent work that suggests that the dynamics (fusion and fission) of these organelles is important in development and disease...
  48. pmc The v-ATPase V0 subunit a1 is required for a late step in synaptic vesicle exocytosis in Drosophila
    P Robin Hiesinger
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 121:607-20. 2005
    ..In addition, an independent function in membrane fusion has been proposed largely based on yeast vacuolar fusion experiments...
  49. pmc Regulation of exocytosis by the exocyst subunit Sec6 and the SM protein Sec1
    Francesca Morgera
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Biol Cell 23:337-46. 2012
    ..regulating soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) complexes and membrane fusion through interactions between the Sec6 subunit and the plasma membrane SNARE protein Sec9...
  50. ncbi Multicolour imaging of post-Golgi sorting and trafficking in live cells
    P Keller
    Cell Biology/Biophysics Programme, European Molecular Biology Laboratory (EMBL, Meyerhofstrasse 1, D-69117 Heidelberg, Germany
    Nat Cell Biol 3:140-9. 2001
    ..Fusion with the plasma membrane was detected by total internal reflection microscopy and reveals differences between apical and basolateral carriers as well as new 'hot spots' for exocytosis...
  51. ncbi Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody
    P D Kwong
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    Nature 393:648-59. 1998
    ..Our results provide a framework for understanding the complex biology of HIV entry into cells and should guide efforts to intervene...
  52. pmc HOPS initiates vacuole docking by tethering membranes before trans-SNARE complex assembly
    Christopher M Hickey
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Mol Biol Cell 21:2297-305. 2010
    ..SNAREs further stabilize the associations of HOPS-tethered membranes. HOPS then protects newly formed trans-SNARE complexes from disassembly by Sec17p/Sec18p...
  53. pmc Structure of a trimeric variant of the Epstein-Barr virus glycoprotein B
    Marija Backovic
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208
    Proc Natl Acad Sci U S A 106:2880-5. 2009
    ..its pre and postfusion states, shed light on gB residues that may be involved in conformational changes and membrane fusion. Also, the EBV gB structure reveals that, despite the high sequence conservation of gB in herpesviruses, the ..
  54. pmc Protein-driven membrane stresses in fusion and fission
    Michael M Kozlov
    Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, 69978 Tel Aviv, Israel
    Trends Biochem Sci 35:699-706. 2010
    ..The biophysical similarities and differences between membrane fusion and fission become more evident if we disregard the accompanying biological processes and consider only ..
  55. pmc Inhibitors of cathepsin L prevent severe acute respiratory syndrome coronavirus entry
    Graham Simmons
    Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 102:11876-81. 2005
    ..system demonstrates that engagement of receptor followed by proteolysis is required for SARS-CoV membrane fusion and indicates that cathepsin L is sufficient to activate membrane fusion by SARS-CoV S...
  56. ncbi Close membrane-membrane proximity induced by Ca(2+)-dependent multivalent binding of synaptotagmin-1 to phospholipids
    Demet Arac
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 13:209-17. 2006
    ..We propose a model wherein synaptotagmin cooperates with the SNAREs in bringing the synaptic vesicle and plasma membranes together and accelerates membrane fusion through the highly positive electrostatic potential of its C(2)B domain.
  57. ncbi Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly
    M Hetzer
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Cell Biol 3:1086-91. 2001
    Although nuclear envelope (NE) assembly is known to require the GTPase Ran, the membrane fusion machinery involved is uncharacterized...
  58. pmc Domain III from class II fusion proteins functions as a dominant-negative inhibitor of virus membrane fusion
    Maofu Liao
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 171:111-20. 2005
    Alphaviruses and flaviviruses infect cells through low pH-dependent membrane fusion reactions mediated by their structurally similar viral fusion proteins...
  59. pmc Membrane fusion catalyzed by a Rab, SNAREs, and SNARE chaperones is accompanied by enhanced permeability to small molecules and by lysis
    Paola C Zucchi
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755 3844, USA
    Mol Biol Cell 22:4635-46. 2011
    ....
  60. pmc Membrane proximal lysosomes are the major vesicles responsible for calcium-dependent exocytosis in nonsecretory cells
    Jyoti K Jaiswal
    Laboratory of Cellular Biophysics, Rockefeller University, New York, NY 10021, USA
    J Cell Biol 159:625-35. 2002
    ..The lysosomes that fused were predominantly predocked at the plasma membrane, indicating that calcium is primarily responsible for fusion and not recruitment of lysosomes to the cell surface...
  61. pmc Distinct contributions of vacuolar Qabc- and R-SNARE proteins to membrane fusion specificity
    Ryota Izawa
    Institute for Protein Research, Osaka University, Suita, Osaka 565 0871, Japan
    J Biol Chem 287:3445-53. 2012
    ..on one membrane and R-SNARE on the opposing membrane assemble into a trans-QabcR-SNARE complex to drive membrane fusion. However, it remains ambiguous whether pairing of Qabc- and R-SNAREs mediates membrane fusion specificity...
  62. ncbi p47 is a cofactor for p97-mediated membrane fusion
    H Kondo
    Cell Biology Laboratory, Imperial Cancer Research Fund, London, UK
    Nature 388:75-8. 1997
    ..As a homologue of p47 exists in budding yeast, this indicates that it might also be involved in other membrane fusion reactions catalysed by p97, such as karyogamy.
  63. pmc Control systems for membrane fusion in the ancestral eukaryote; evolution of tethering complexes and SM proteins
    V Lila Koumandou
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
    BMC Evol Biol 7:29. 2007
    ..This pattern is also likely for Rabs and various other components of the membrane trafficking machinery...
  64. ncbi EEA1 links PI(3)K function to Rab5 regulation of endosome fusion
    A Simonsen
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, Oslo
    Nature 394:494-8. 1998
    ....
  65. pmc Enhanced growth of influenza vaccine seed viruses in vero cells mediated by broadening the optimal pH range for virus membrane fusion
    Shin Murakami
    Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    J Virol 86:1405-10. 2012
    ..This mutation optimized the pH for HA-mediated membrane fusion in Vero cells and enhanced virus growth 100 to 1,000 times in the cell line, providing a promising strategy ..
  66. pmc Low-pH-induced membrane fusion mediated by human metapneumovirus F protein is a rare, strain-dependent phenomenon
    Sander Herfst
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Virol 82:8891-5. 2008
    b>Membrane fusion promoted by human metapneumovirus (HMPV) fusion (F) protein was suggested to require low pH (R. M. Schowalter, S. E. Smith, and R. E. Dutch, J. Virol. 80:10931-10941, 2006)...
  67. ncbi Fast vesicle fusion in living cells requires at least three SNARE complexes
    Ralf Mohrmann
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Science 330:502-5. 2010
    ..Heterogeneity in SNARE-complex number may explain heterogeneity in vesicular release probability...
  68. ncbi Myosin II contributes to fusion pore expansion during exocytosis
    Patricia Neco
    Departamento Fisiología Médica y Biofísica, Universidad de Sevilla, Sevilla 41009, Spain
    J Biol Chem 283:10949-57. 2008
    ..We proved that myosin II acts as a molecular motor on the fusion pore expansion by hindering its dilation when it lacks the phosphorylation sites...
  69. pmc SNARE complexes of different composition jointly mediate membrane fusion in Arabidopsis cytokinesis
    Farid El Kasmi
    Developmental Genetics, Center for Plant Molecular Biology, University of Tubingen, 72076 Tubingen, Germany
    Mol Biol Cell 24:1593-601. 2013
    b>Membrane fusion is mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes...
  70. pmc Doc2b is a high-affinity Ca2+ sensor for spontaneous neurotransmitter release
    Alexander J Groffen
    Department of Functional Genomics, CNCR, Neuroscience Campus Amsterdam, VU University and VU Medical Center, Amsterdam, 1081 HV, Netherlands
    Science 327:1614-8. 2010
    ....
  71. pmc Rab8a regulates the exocyst-mediated kiss-and-run discharge of the Dictyostelium contractile vacuole
    Miriam Essid
    Department de Biochimie, Faculte des Sciences, Universite de Geneve, Geneva, Switzerland
    Mol Biol Cell 23:1267-82. 2012
    ..An indistinguishable phenotype is generated in cells lacking LvsA, implicating this protein in postfusion detethering. Of interest, overexpression of a constitutively active Rab8a mutant reverses the lvsA-null CV phenotype...
  72. pmc The Legionella pneumophila effector DrrA is sufficient to stimulate SNARE-dependent membrane fusion
    Kohei Arasaki
    Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06526, USA
    Cell Host Microbe 11:46-57. 2012
    ..Rab1-targeting effector DrrA is sufficient to stimulate this noncanonical SNARE association and promote membrane fusion. DrrA activation of the Rab1 GTPase on PM-derived organelles stimulated the tethering of ER-derived vesicles ..
  73. pmc The PINK1/Parkin pathway regulates mitochondrial morphology
    Angela C Poole
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 105:1638-43. 2008
    ..Our studies suggest that the PINK1/Parkin pathway promotes mitochondrial fission and that the loss of mitochondrial and tissue integrity in PINK1 and parkin mutants derives from reduced mitochondrial fission...
  74. pmc Endosome-to-cytosol transport of viral nucleocapsids
    Isabelle Le Blanc
    Biochemistry Department, University of Geneva, 30 quai E Ansermet, 1211 Geneva 4, Switzerland
    Nat Cell Biol 7:653-64. 2005
    ....
  75. ncbi Three-dimensional structure of the complexin/SNARE complex
    Xiaocheng Chen
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Neuron 33:397-409. 2002
    ..bundle, the SNARE complex, that pulls the synaptic vesicle and plasma membranes together possibly causing membrane fusion. Complexin binds tightly to the SNARE complex and is essential for efficient Ca(2+)-evoked neurotransmitter ..
  76. ncbi Solvent-exposed tails as prestalk transition states for membrane fusion at low hydration
    Yuliya G Smirnova
    Max Planck Institute of Colloids and Interfaces, Department of Theory and Bio Systems, Research Campus Golm, D 14424 Potsdam, Germany
    J Am Chem Soc 132:6710-8. 2010
    b>Membrane fusion is a key step in intracellular trafficking and viral infection. The underlying molecular mechanism is poorly understood...
  77. ncbi Ugo1 and Mdm30 act sequentially during Fzo1-mediated mitochondrial outer membrane fusion
    Fabian Anton
    Institute for Genetics, University of Cologne, 50674 Cologne, Germany
    J Cell Sci 124:1126-35. 2011
    ..Our results thus unravel functions of Ugo1 and Mdm30 at distinct steps during OM fusion and suggest that protein clearance confers a non-cycling mechanism to mitofusins, which is distinct from other cellular membrane fusion events.
  78. ncbi Membrane fusion: SNAREs and regulation
    J Malsam
    Biochemistry Center, University Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    Cell Mol Life Sci 65:2814-32. 2008
    SNARE (SNAP receptor) proteins drive intracellular membrane fusion and contribute specificity to membrane trafficking...
  79. pmc Intersubunit interactions modulate pH-induced activation of membrane fusion by the Junin virus envelope glycoprotein GPC
    Joanne York
    Montana Biotechnology Center, The University of Montana, Missoula, Montana 59812, USA
    J Virol 83:4121-6. 2009
    ..We have shown previously that SSP is a key element in GPC-mediated membrane fusion, and that GPC sensitivity to acidic pH is modulated in part through the lysine residue at position 33 in the ..
  80. ncbi Functions and dysfunctions of mitochondrial dynamics
    Scott A Detmer
    Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
    Nat Rev Mol Cell Biol 8:870-9. 2007
    ..But what are the molecular mechanisms that control mitochondrial dynamics, and why are they important for mitochondrial function? We review these issues and explore how defects in mitochondrial dynamics might cause neuronal disease...
  81. pmc Separate fusion of outer and inner mitochondrial membranes
    Florence Malka
    INSERM U582, Institut de Myologie, Universite Pierre et Marie Curie, IFR14, Groupe Hospitalier Pitie Salpetriere, 47 Blvd de l Hopital, 75651 Paris Cedex 13, France
    EMBO Rep 6:853-9. 2005
    ..We set up assays for the study of inner and outer membrane fusion in living human cells...
  82. pmc Human immunodeficiency virus type 1 Env with an intersubunit disulfide bond engages coreceptors but requires bond reduction after engagement to induce fusion
    L G Abrahamyan
    Department of Molecular Biophysics and Physiology, Rush Medical College, Chicago, Illinois 60612, USA
    J Virol 77:5829-36. 2003
    ..The capture of this configuration of Env could yield a suitable antigen for vaccine development, and it may also be a target for pharmacological intervention against HIV-1 entry...
  83. ncbi Rim is a putative Rab3 effector in regulating synaptic-vesicle fusion
    Y Wang
    Department of Molecular Genetics, The University of Texas, Southwestern Medical Center at Dallas, 75235, USA
    Nature 388:593-8. 1997
    ..We propose that Rim serves as a Rab3-dependent regulator of synaptic-vesicle fusion by forming a GTP-dependent complex between synaptic plasma membranes and docked synaptic vesicles...
  84. pmc Coiled-coil domains in glycoproteins B and H are involved in human cytomegalovirus membrane fusion
    Matthew Lopper
    Department of Biomolecular Chemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    J Virol 78:8333-41. 2004
    ..Three conserved viral glycoproteins, gB, gH, and gL, are required for CMV-mediated membrane fusion, but little is known of how these proteins cooperate during entry (E. R. Kinzler and T...
  85. pmc Regulation of human immunodeficiency virus type 1 Env-mediated membrane fusion by viral protease activity
    Tsutomu Murakami
    Department of Immunology, Graduate School and Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903 0215, Japan
    J Virol 78:1026-31. 2004
    ..change following Gag processing, and the cytoplasmic tail of gp41 has been shown to modulate Env-mediated membrane fusion activity...
  86. pmc SNAREs are concentrated in cholesterol-dependent clusters that define docking and fusion sites for exocytosis
    T Lang
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    EMBO J 20:2202-13. 2001
    ..This suggests that high local concentrations of SNAREs are required for efficient fusion...
  87. pmc Unconventional myosin Myo1c promotes membrane fusion in a regulated exocytic pathway
    Avirup Bose
    Program in Molecular Medicine, Department of Physiology, University of Massachusetts Medical School, Worcester, 01605, USA
    Mol Cell Biol 24:5447-58. 2004
    ..Myo1c expression under these conditions causes extensive membrane ruffling and overrides the block in membrane fusion caused by LY294002, restoring the display of GLUT4 on the cell exterior...
  88. ncbi The mechanisms of vesicle budding and fusion
    Juan S Bonifacino
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 116:153-66. 2004
    ..Precise regulation of these two aspects of vesicular transport ensures efficient cargo transfer while preserving organelle identity...
  89. pmc Bimolecular complementation reveals that glycoproteins gB and gH/gL of herpes simplex virus interact with each other during cell fusion
    Doina Atanasiu
    Department of Microbiology, School of Dental Medicine, and Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104
    Proc Natl Acad Sci U S A 104:18718-23. 2007
    ..Thus, when gD binds its receptor, the core fusion machinery is triggered to form a multiprotein complex as a step in fusion and possibly virus entry...
  90. pmc Structure of the uncleaved ectodomain of the paramyxovirus (hPIV3) fusion protein
    Hsien Sheng Yin
    Department of Biochemistry, Molecular Biology, and Cell Biology, Howard Hughes Medical Institute, Northwestern University, Evanston, IL 60208 3500, USA
    Proc Natl Acad Sci U S A 102:9288-93. 2005
    ..Structural insights into the protein conformational changes associated with membrane fusion are based largely on studies of the influenza virus hemagglutinin in pre- and postfusion conformations...
  91. ncbi Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion
    H Stenmark
    European Molecular Biology Laboratory, Heidelberg Federal Republic of Germany
    Cell 83:423-32. 1995
    ..Immunodepletion of rabaptin-5 from cytosol strongly inhibits Rab5-dependent early endosome fusion. Rabaptin-5 is thus a Rab effector required for membrane docking and fusion...
  92. pmc Membrane raft microdomains mediate lateral assemblies required for HIV-1 infection
    S Manes
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia CSIC, Madrid, Spain
    EMBO Rep 1:190-6. 2000
    ..These results indicate a general mechanism for HIV-1 envelope glycoprotein-mediated fusion by reorganization of membrane microdomains in the target cell, and offer new strategies for preventing HIV-1 infection...
  93. ncbi Amino acids from both N-terminal hydrophobic regions of the Lassa virus envelope glycoprotein GP-2 are critical for pH-dependent membrane fusion and infectivity
    Christian Klewitz
    The Institute for Virology, Philipps University, Hans Meerwein Strasse 3, 35043 Marburg, Germany
    J Gen Virol 88:2320-8. 2007
    ..The data demonstrate that several amino acids from both hydrophobic regions of the N terminus, some of which (W264, G277, Y278 and L280) are 100 % conserved in all arenaviruses, are involved in fusogenicity and infectivity of LASV GP-2...
  94. pmc pH-induced activation of arenavirus membrane fusion is antagonized by small-molecule inhibitors
    Joanne York
    Montana Biotechnology Center, The University of Montana, Science Complex, Missoula, Montana 59812, USA
    J Virol 82:10932-9. 2008
    The arenavirus envelope glycoprotein (GPC) mediates viral entry through pH-induced membrane fusion in the endosome...
  95. ncbi The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway
    C Bucci
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 70:715-28. 1992
    ..We conclude that rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway...
  96. pmc Analysis of residues near the fusion peptide in the influenza hemagglutinin structure for roles in triggering membrane fusion
    Sudha Thoennes
    Department of Microbiology and Immunology, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    Virology 370:403-14. 2008
    ..triggers irreversible conformational changes of the hemagglutinin glycoprotein (HA) that are required for membrane fusion. The acid-induced HA structural rearrangements have been well documented, and several models have been ..
  97. ncbi Molecular view of the role of fusion peptides in promoting positive membrane curvature
    Marc Fuhrmans
    Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands
    J Am Chem Soc 134:1543-52. 2012
    Fusion peptides are moderately hydrophobic segments of viral and nonviral membrane fusion proteins that enable these proteins to fuse two closely apposed biological membranes...
  98. ncbi Caught in the act: visualization of SNARE-mediated fusion events in molecular detail
    Herre Jelger Risselada
    Theoretical Molecular Biophysics Group, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Chembiochem 12:1049-55. 2011
    ..SNAREs are the core constituents of the protein machinery responsible for this membrane fusion, but the actual fusion mechanism remains unclear...
  99. pmc Microscopic observations reveal that fusogenic peptides induce liposome shrinkage prior to membrane fusion
    Fumimasa Nomura
    Department of Molecular Biology, School of Science, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8602, Japan
    Proc Natl Acad Sci U S A 101:3420-5. 2004
    To study the mechanisms involved in membrane fusion, we visualized the fusion process of giant liposomes in real time by optical dark-field microscopy...
  100. pmc A conformation-specific monoclonal antibody reacting with fusion-active gp41 from the human immunodeficiency virus type 1 envelope glycoprotein
    S Jiang
    Lindsley F Kimball Research Institute, New York Blood Center, New York, New York 10021, USA
    J Virol 72:10213-7. 1998
    ..subunit of the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein plays a major role in the membrane fusion step of viral infection...
  101. pmc Membrane fusion intermediates via directional and full assembly of the SNARE complex
    Javier M Hernandez
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Science 336:1581-4. 2012
    Cellular membrane fusion is thought to proceed through intermediates including docking of apposed lipid bilayers, merging of proximal leaflets to form a hemifusion diaphragm, and fusion pore opening...

Research Grants73

  1. Sphingolipid Metabolism in Drosophila Development
    Julie D Saba; Fiscal Year: 2013
    ..influence key processes involved in muscle development and regeneration, including myogenic gene expression, membrane fusion, satellite cell activation, muscle membrane structure and extracellular matrix processing...
  2. Human cytomegalovirus entry into epithelial and endothelial cells
    David C Johnson; Fiscal Year: 2013
    ..Moreover, we will study the molecular mechanisms of gB and gH/gL-mediated membrane fusion. Other efforts will focus on the identification of novel cellular receptors that will explain clinical HCMV ..
  3. Disease-related defects in dendritic cell processing of bacterial antigens
    Michael S Marks; Fiscal Year: 2012
    ..and eventually killed as the phagosome in which they are sequestered matures through an ordered series of membrane fusion and fission events, leading to acidification, consequent activation of hydrolases, and synthesis of reactive ..
  4. Cellular Components Involved in Japanese Encephalitis Virus Entry
    Young Min Lee; Fiscal Year: 2013
    ..which attaches the virions to the cell surface, delivers them to endosomes, and catalyzes the viral-endosome membrane fusion), the host factors that participate in this multistep process remain elusive...
  5. Mechanistic Studies of HCV E2
    Joseph Marcotrigiano; Fiscal Year: 2013
    ..In addition to its role in membrane fusion, E2 is thought to be responsible for targeting since it has been shown to bind host cellular receptors CD81 ..
  6. Phosphoprotein CDP138 regulates glucose metabolism
    ZHEN YUE JIANG; Fiscal Year: 2013
    ..domain and Akt phosphorylation site Ser197 in CDP138 are critical for insulin-induced GLUT4 translocation and membrane fusion between the GLUT4 vesicles and the PM. We have also successfully developed the first CDP138 mutant mouse line...
  7. 2011 Cell-Cell Fusion Gordon Research Conference and Seminar
    NANCY RYAN GRAY; Fiscal Year: 2011
    ..fusion during health and disease as well as the relationship between cell fusion and the larger fields of membrane fusion and fission during exocytosis, endocytosis, viral entry, and cytokinesis...
  8. Cardiac function and PIP2
    Donald W Hilgemann; Fiscal Year: 2013
    ..b>Membrane fusion and budding processes are fundamental to all eukaryotic life, and we have developed improved ..
  9. MEMBRANE FUSION IN RETINAL ROD OUTER SEGMENTS
    KATHLEEN BOESZEBATTAGLIA; Fiscal Year: 2012
    ..Our studies should reveal novel insights into the pathogenesis of peripherin/rds mediated retinal dystrophies and form the underpinnings for the development of therapeutic strategies to treat these diseases. ..
  10. Cloning and Analysis of a Gamete Fusion Gene
    CHARLENE FOREST; Fiscal Year: 2009
    ..independently and where mutations can therefore be defined as affecting plasma membrane adhesion or plasma membrane fusion. Using our insertional mutants, TAIL-PCR (Thermal asymmetric interlaced-PCR) has allowed us to identify 2 ..
  11. MOLECULAR MECHANISMS OF ALPHAVIRUS ENTRY AND EXIT
    Margaret Kielian; Fiscal Year: 2013
    ..and flaviviruses are small enveloped plus-sense RNA viruses with structurally and functionally-related membrane fusion proteins...
  12. Measles Virotherapy and radiovirotherapy in the Treatment of recurrent gliomas
    Evanthia Galanis; Fiscal Year: 2013
    ..The virus upon entry in the tumor cells, causes membrane fusion with neighboring cells, syncytia formation and death...
  13. Inositol Lipid regulation of membrane fusion and fission
    Lois S Weisman; Fiscal Year: 2013
    ..Results of these studies should provide new insights into the roles and regulation of PI3,5P2 and will provide a basis for determining to roles of PI3,5P2 in the nervous system. ..
  14. Facilitating large scale biomass generation: acquisition of an automated 100 L fe
    Roger D Kornberg; Fiscal Year: 2010
    ..adhesion through the surface receptor, and binding of regulatory proteins to the SNAREs responsible for cell membrane fusion. All of this work entails X-rays crystallographic analysis, and depends on bacterial fermentation...
  15. Structural Studies of Alphavirus Membrane Fusion In Situ
    Wei Zhang; Fiscal Year: 2010
    ..the structural details of the intermediate organizations of the fusion protein during the course of membrane fusion are poorly understood...
  16. Mechanisms of Neuronal Spread of Neurotropic Mouse Hepatitis Virus
    Judith M Phillips; Fiscal Year: 2013
    ..spread across synapses, release of virus from neuronal cell bodies and axons in compartmented cultures, and membrane fusion of infected neurons of JHM, A59, rA59/SJHM (a chimeric A59 virus with the JHM spike gene), and, if relevant, ..
  17. Gamete membrane adhesion and fusion during fertilization
    William J Snell; Fiscal Year: 2013
    ..Understanding, for at least one organism, the molecular events that occur during the gamete membrane fusion reaction will have a large impact on the field of reproductive biology...
  18. Lipid Signaling During Fertilization
    Bradley J Stith; Fiscal Year: 2010
    ..by applicant): There is numerous membrane events associated with fertilization: phospholipids metabolism, membrane fusion during the sperm acrosome reaction, during sperm-egg fusion, during cortical granule exocytosis, and changes ..
  19. STRUCTURAL BASIS OF ORGANELLE TETHERING
    Adam D Linstedt; Fiscal Year: 2013
    ..of tethering upon membrane binding, promotion of trans interactions, and coordination with SNARE-mediate membrane fusion. Our working model is that GM130 recruits GRASP65 to cis cisternae using a C-terminal PDZ ligand to bind the ..
  20. Nanoparticle Analysis of Enveloped Virus Entry Pathways
    Robert Davey; Fiscal Year: 2009
    ..Envs are also simple membrane fusion engines that sense the surrounding environment to trigger the release of their cargo in the cell cytoplasm at ..
  21. REGULATION OF NUCLEAR PORE COMPLEX ASSEMBLY
    Susan R Wente; Fiscal Year: 2011
    ..Together, these studies will define the membrane fusion machinery and the sequence of self-assembly steps for NPC biogenesis in intact NEs...
  22. Mechanisms of Nuclear and Cell Fusion in Yeast
    MARK DAVID ROSE; Fiscal Year: 2013
    ..mating-induced protein, couples the inner and outer nuclear membranes during fusion and facilitates inner-membrane fusion. We will use advanced imaging and genetic methods to identify Kar5p's role(s) in nuclear membrane fusion. ..
  23. Computational and Functional Characterization of the Molecular Steps in Membran*
    J Troy Littleton; Fiscal Year: 2013
    ..in NMJs where the fusion machinery is modified by point mutations with computationally predicted effects on membrane fusion. This research will be performed by a multidisciplinary team that includes experts in molecular modeling (Dr...
  24. Hepatitis C: E2 Molecular Mechanisms
    Martina Buck; Fiscal Year: 2013
    ..1, and occludin) that are indispensible for initial viral binding to the cellular membrane and subsequent membrane fusion mechanisms that lead to viral entry...
  25. Mitochondrial Functions of Dynamin-Related Proteins
    Alexander M van der Bliek; Fiscal Year: 2012
    ..mediates fission, Mitofusins are mitochondrial outer membrane dynamin-related proteins that mediate outer membrane fusion and Opa1 is an intermembrane space dynamin- related protein that mediates inner membrane fusion...
  26. The Role of Bovine Sperm Acrosomal Membrane-Matrix Complex in Fertilization
    Subir K Nagdas; Fiscal Year: 2013
    ..domain, and its adherent acrosomal matrix, and to define the role of their constituent polypeptides in the membrane fusion, and the release of hydrolases during the acrosome reaction...
  27. Allosteric Regulation of SNAREs and the Rab GEF Mon1 by Phosphoinositides
    Rutilio A Fratti; Fiscal Year: 2013
    ..organelles, which communicate with each other through the transport of cargo vesicles that culminates in membrane fusion. We use vacuolar lysosomes isolated from the yeast Saccharomyces cerevisiae to examine membrane fusion...
  28. Effects of saliva on herpes simplex virus infection of oralcells
    CLAUDE F KRUMMENACHER; Fiscal Year: 2013
    ..lines to define how saliva stimulates HSV-1 entry at the level of receptor interaction, entry pathways and membrane fusion. Finally, to relate this effect to in vivo infection, we will survey primary human oral cells and HSV ..
  29. Modulation of calcium signaling by changes in STIM expression
    Jonathan Soboloff; Fiscal Year: 2013
    ..signal transduction pathways regulating a wide variety of responses ranging from rapid events such as membrane fusion and muscle contraction to control of proliferation, differentiation and apoptosis...
  30. Synaptotagmin and C2-Domains: Structure and Function
    JOSE RIZO-REY; Fiscal Year: 2013
    ..Aim 1 will continue ongoing studies directed at elucidating how synaptotagmin-1 triggers membrane fusion and neurotransmitter release in a Ca2+-dependent manner together with SNARE proteins and in a tight interplay ..
  31. Modeling viral entry and its inhibition using SARS-CoV
    Graham Simmons; Fiscal Year: 2012
    ..We aim to elucidate the mechanisms by which such enveloped viruses mediate membrane fusion during the process of entry...
  32. Interactions of Ebola virus glycoproteins with host cells
    Paul Bates; Fiscal Year: 2013
    ..entry of the virus into the host and is believed to be responsible for attachment, receptor recognition, and membrane fusion. Definition of the host factors that are required for Ebola entry is essential for comprehending Ebola virus ..
  33. Optical Study of Secretion in Mammalian Nerve Terminals
    BRIAN MATTHEW SALZBERG; Fiscal Year: 2013
    ..direct observation in real time of the intraterminal events that follow electrical excitation, but precede membrane fusion, has proven extremely difficult...
  34. Membrane Remodeling and Protein Trafficking in Bacteria
    David Z Rudner; Fiscal Year: 2013
    ..Virtually nothing is known about how bacteria catalyze membrane fusion despite the fact that in every cell division the invaginating septal membranes fuse (resulting in binary ..
  35. STRUCTURE AND ASSEMBLY OF VIRUSES
    STEPHEN COPLAN HARRISON; Fiscal Year: 2012
    ..In so doing, we seek to establish mechanisms for quite general processes, such as membrane fusion and perforation, as well as to lay the groundwork for discovering novel classes of antiviral therapeutics and ..
  36. ELEXSYS E500 CW EPR SPECTROMETER
    Jack H Freed; Fiscal Year: 2010
    ..proteins: We will test the hypothesis that increased bilayer ordering is associated with more robust membrane fusion, by comparing the bilayer ordering effect of more fusogenic mutants of wt20 vs. wt20...
  37. Biophysical Characterization of Complexin C-terminal Domain Lipid Interactions
    David M Snead; Fiscal Year: 2013
    ..This highly regulated process is mediated by a core membrane fusion machinery consisting of the SNARE proteins, and is further modulated by a number of accessory proteins...
  38. Poliovirus Cell Entry Pathways
    James M Hogle; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Membrane fusion provides a conceptually simple mechanism for enveloped viruses to deliver their genomes into the cytoplasm of target cells...
  39. SARS Coronavirus: Inhibition of Entry
    Kathryn Holmes; Fiscal Year: 2009
    ..what parts of the protein bind to the receptor, where antibodies target the spike, and how the spike causes membrane fusion. Dr. Wentworth (Project 5) and Dr...
  40. Structural Analysis of Golgi Trafficking Proteins
    Frederick M Hughson; Fiscal Year: 2013
    ..Finally, membrane fusion at late endosomes and lysosomes/vacuoles depends on the HOPS complex...
  41. Functional mapping of SNARE-dependent trafficking and fusion
    Jeffrey E Pessin; Fiscal Year: 2013
    ..Moreover, the basic biophysical mechanism of biological membrane fusion for this, or any other system, has not been elucidated...
  42. Biochemical analysis of the ER fusion protein allastin
    James A McNew; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Membrane fusion is vitally important for many aspects of eukaryotic cell biology including vesicular traffic within the secretory pathway as well as the biogenesis and maintenance of the entire ..
  43. Novel Regulators of Endothelial Exocytosis
    Charles J Lowenstein; Fiscal Year: 2013
    ..will study the role of candidate gene products in granule biogenesis, cargo loading, vesicle trafficking, and membrane fusion. In addition, we will also study 5 candidate genes previously identified in a genome wide association study ..
  44. Molecular Studies of the Roles and Regulations of the v-ATPase V0 Sector
    Florante A Quiocho; Fiscal Year: 2010
    ..provided by applicant): The V0 sector subunit "a" has been shown to play important roles in diverse membrane fusion or secretion functions independent of the V-ATPase proton-pumping activity...
  45. Role of Rab Proteins in Golgi Apparatus Structure and Function
    Brian Storrie; Fiscal Year: 2013
    ..vesicle targeting via the recruitment and activation of tethering factors that mediate initial steps of membrane fusion. Our Preliminary Studies support a different and novel mechanism...
  46. Mitochondrial Fusion and Division
    Hiromi Sesaki; Fiscal Year: 2013
    ..Using these proteins, we have developed assays for GTP binding, GTP hydrolysis, and GTP-dependent membrane fusion. These novel assays will allow us to dissect the functions of Fzo1p GTPase and Ugo1p in mitochondrial fusion...
  47. Rational Design of Viral-Mimetic Lipid Gene Vectors
    Francis C Szoka; Fiscal Year: 2013
    ..We postulate that molecular strategies to deliver nucleic acids by viral membrane fusion proteins can be mimicked using less complex lipids...
  48. Targeting Viral Envelope Glycoproteins with Synthetic Antibodies
    Jonathan Lai; Fiscal Year: 2013
    ..this proposal is to develop enabling synthetic antibody technologies for applications in the study of viral membrane fusion. In Aim 1, we propose to develop synthetic antibody libraries focused toward viral antigens based on the ..
  49. The molecular mechanisms of mitochondrial behavior.
    Suzanne C Hoppins; Fiscal Year: 2012
    ..In order to understand the functional significance of two outer membrane fusion proteins and the regulatory mechanisms that govern their activity, we propose to characterize the biochemical ..
  50. TorsinA and Nuclear Envelope Function in HSV Infection
    Richard J Roller; Fiscal Year: 2013
    ..between torsinA and herpes simplex type 1, and evidence specifically for a role for torsinA in regulation of membrane fusion at the nuclear envelope...
  51. Solid-State NMR of Viral Fusion Peptides and Proteins
    David P Weliky; Fiscal Year: 2013
    ..The long-term goal of the proposed research is to understand the basis for viral fusion protein-induced membrane fusion with atomic-resolution detail...
  52. Epididymal PMCA4 Expression Functional Impact and Mechanism of Sperm Uptake
    PATRICIA ANASTASIA DELEON; Fiscal Year: 2013
    ..sperm surface, assisted by the presence of CD9 tetraspanin which mediates cell-to-cell protein transfer via membrane fusion. Sperm PM - vesicle interaction will be visualized in ultrastructural images using immunogold labeling to ..
  53. Broad spectrum therapeutics that target the viral membrane
    Benhur Lee; Fiscal Year: 2012
    ..Michael Jung (co-Pi), the virus-cell membrane fusion expertise of the Dr. Benhur Lee (PI), and the biodefense and BSL4 virological expertise of Dr...
  54. FORESPORE ENGULFMENT DURING B SUBTILIS SPORULATION
    KIT J POGLIANO; Fiscal Year: 2013
    ..system to study cell biological events that are essential for all bacteria, such as protein localization and membrane fusion. PUBLIC HEALTH RELEVANCE: Peptidoglycan hydrolases are found in all bacteria that synthesize peptidoglycan, ..
  55. Hermansky Pudlak Syndrome &melanosome formation
    GRACA GRACA RAPOSO; Fiscal Year: 2012
    ..AP-3 function in concert with Rab11 to regulating recycling endosome membrane dynamics and SNARE-dependent membrane fusion with maturing melanosomes, and that syntaxin 13 and VAMP7 are components of the requisite SNARE complexes...
  56. High Throughput Screening for Modulators of the TRPML1 Ion Channel
    Haoxing Xu; Fiscal Year: 2013
    ..by transducing information about PI(3,5)P2 levels into changes in juxtaorganellar Ca2+, thereby triggering membrane fusion/fission events in the late endocytic pathways (endolysosomal trafficking)...
  57. PHOSPHOLIPID-DERIVED MEDIATORS AND INSULIN SECRETION
    John W Turk; Fiscal Year: 2010
    ..We have also found that arachidonate-containingplasmalogens, which participate in membrane fusion and exocytosis, are abundant in p-cells, and these ether lipids are produced from peroxisome- derived ..
  58. Characterization of nucleotide-dependent structural rearrangements in atlastin
    LAURA JEAN BYRNES; Fiscal Year: 2012
    ..atlastin-1 is involved in generating the tubular endoplasmic reticulum (ER) network by facilitating homotypic membrane fusion. Those dynamin-related G proteins that catalyze homotypic fusion of biological membranes comprise a small ..
  59. CENTER FOR BIOMOLECULAR STRUCTURE AND DYNAMICS
    Stephen R Sprang; Fiscal Year: 2011
    ..NMR experiments will be used to elucidate the structures of critical domains in the Junin arenavirus membrane fusion glycoprotein...
  60. MITOCHONDRIAL MANIPULATION AND ANALYSIS IN DROSOPHILA HEARTS
    Gerald W Dorn; Fiscal Year: 2012
    ..revised R21 we propose studies that will use in vivo genetic manipulation of mitochondrial outer and inner membrane fusion proteins to define the role of mitochondrial fusion in Drosophila heart tubes...
  61. CONTROL OF VIRUS INDUCED LYSIS
    RYLAND F YOUNG; Fiscal Year: 2013
    ..A model has been advance that the destruction of the OM is the result of membrane fusion with the IM, thus removing the last barrier to virus release. The proposal focuses on two specific areas...
  62. MOLECULAR ANALYSIS OF ALPHAVIRUS MEMBRANE FUSION PROTEIN
    Margaret Kielian; Fiscal Year: 2013
    ..Alphaviruses and flaviviruses infect cells by a membrane fusion reaction that is triggered by low pH during virus endocytic uptake and mediated by structurally similar ..
  63. MOLECULAR ANALYSIS OF ALPHAVIRUS MEMBRANE FUSION PROTEIN
    Margaret Kielian; Fiscal Year: 2006
    ..Both alphaviruses and flaviviruses infect cells via a low pH-dependent membrane fusion reaction...
  64. Priming and fusion activation of the SARS coronavirus spike glycoprotein
    Gary R Whittaker; Fiscal Year: 2010
    ..many unanswered questions regarding the process of SARS-CoV entry into host cells, especially with regard to membrane fusion events...
  65. Genetics and Functions of HSV Membrane Fusion Proteins
    KONSTANTIN KOUSOULAS; Fiscal Year: 2009
    ..In addition, both UL20p and gK harbor syncytial mutations suggesting that they are involved in membrane fusion phenomena during virion envelopment and egress, as well as virus-induced cell fusion...
  66. UNDERSTANDING MEMBRANE FUSION A BIOENGINEERING APPROACH
    BHANU JENA; Fiscal Year: 2001
    ..a "new structure" associated with the neuronal fusion machinery has been identified and its participation in membrane fusion is suggested...
  67. Molecular Mechanisms of Rhabdovirus Entry
    Sean Whelan; Fiscal Year: 2009
    ..changes that bring the viral and target membranes into close proximity, lowering the energy barrier to membrane fusion. The mechanism by which class III proteins accomplish this is the least well understood...
  68. INTERMEDIATES AND TRANSBILAYER PEPTIDES
    Martin Caffrey; Fiscal Year: 2001
    DESCRIPTION: Membrane fusion is a critical and ubiquitous phenomenon in biology. However, the mechanism of biomembrane fusion remains one of the central mysteries of membrane biology...
  69. Functional Reconstitution of Yeast Exocytosis
    James A McNew; Fiscal Year: 2010
    b>Membrane fusion is a fundamental process involved in diverse cellular events such as fertilization and neurosecretion...
  70. The Role of Actin Remodeling in Membrane Fusion Microdomains
    William Wickner; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): Regulated membrane fusion is essential for cell compartmentation and growth, neurotransmission, and hormone release...
  71. BIOPHYSICS OF INFLUENZA HEMAGGLUTININ-MEDIATED FUSION
    FREDRIC COHEN; Fiscal Year: 2001
    b>Membrane fusion is a ubiquitous cellular process, but the proteins responsible for fusion have been unambiguously identified only in the case of enveloped virus. For enveloped virus, infection of cells is initiated by membrane fusion...
  72. Molecular Analysis of Murine Leukemia Virus Entry
    Lorraine Albritton; Fiscal Year: 2007
    ..entry by an atypical endocytic pathway, as well as entry by cell surface fusion and a much slower rate of membrane fusion. Lack of information on the molecular basis for these anomalies has hampered an understanding of envelope ..
  73. Membrane fusion during HSV-1 entry
    ROBERT JAMES GERAGHTY; Fiscal Year: 2010
    ..Our long-term goal is to understand the molecular events occurring during HSV-1-induced membrane fusion for both direct fusion and RME...