cell degranulation

Summary

Summary: The process of losing secretory granules (SECRETORY VESICLES). This occurs, for example, in mast cells, basophils, neutrophils, eosinophils, and platelets when secretory products are released from the granules by EXOCYTOSIS.

Top Publications

  1. ncbi CD107a as a functional marker for the identification of natural killer cell activity
    Galit Alter
    Partners AIDS Research Center, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Massachusetts, USA
    J Immunol Methods 294:15-22. 2004
  2. ncbi Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation
    Michael R Betts
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
    J Immunol Methods 281:65-78. 2003
  3. ncbi Mast cell degranulation induced by two phospholipase A2 homologues: dissociation between enzymatic and biological activities
    E C Landucci
    Department of Pharmacology, Faculty of Medical Sciences, UNICAMP, Campinas SP, Brazil
    Eur J Pharmacol 343:257-63. 1998
  4. ncbi Neutrophil granules and secretory vesicles in inflammation
    Mikkel Faurschou
    The Granulocyte Research Laboratory, Department of Haematology, Rigshospitalet 4042, University of Copenhagen, 9 Blegdamsvej, DK 2100 Copenhagen OE, Denmark
    Microbes Infect 5:1317-27. 2003
  5. ncbi Mast-cell degranulation induced by physical stimuli involves the activation of transient-receptor-potential channel TRPV2
    D Zhang
    JWG University Frankfurt, Institute for Biophysics, Frankfurt am Main, Germany
    Physiol Res 61:113-24. 2012
  6. pmc CD4+CD25+ regulatory T cells suppress mast cell degranulation and allergic responses through OX40-OX40L interaction
    Giorgia Gri
    Department of Biomedical Science and Technology, University of Udine, 33100 Udine, Italy
    Immunity 29:771-81. 2008
  7. ncbi Munc13-4 is essential for cytolytic granules fusion and is mutated in a form of familial hemophagocytic lymphohistiocytosis (FHL3)
    Jerome Feldmann
    INSERM U429, Hopital Necker Enfants Malades, 75743 Paris, France
    Cell 115:461-73. 2003
  8. ncbi Corticotropin-releasing hormone induces skin mast cell degranulation and increased vascular permeability, a possible explanation for its proinflammatory effects
    T C Theoharides
    Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Endocrinology 139:403-13. 1998
  9. pmc Transactivation of sphingosine-1-phosphate receptors by FcepsilonRI triggering is required for normal mast cell degranulation and chemotaxis
    Puneet S Jolly
    Department of Biochemistry, Virginia Commonwealth University Medical Center, Richmond 23298, USA
    J Exp Med 199:959-70. 2004
  10. pmc JNK1 controls mast cell degranulation and IL-1{beta} production in inflammatory arthritis
    Monica Guma
    Laboratory of Gene Regulation and Signal Transduction, Department of Pharmacology and Pathology, Division of Rheumatology, School of Medicine, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 107:22122-7. 2010

Detail Information

Publications337 found, 100 shown here

  1. ncbi CD107a as a functional marker for the identification of natural killer cell activity
    Galit Alter
    Partners AIDS Research Center, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Massachusetts, USA
    J Immunol Methods 294:15-22. 2004
    ..lysosomal-associated membrane protein-1 (LAMP-1 or CD107a) has been described as a marker of CD8+ T-cell degranulation following stimulation...
  2. ncbi Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation
    Michael R Betts
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
    J Immunol Methods 281:65-78. 2003
    ....
  3. ncbi Mast cell degranulation induced by two phospholipase A2 homologues: dissociation between enzymatic and biological activities
    E C Landucci
    Department of Pharmacology, Faculty of Medical Sciences, UNICAMP, Campinas SP, Brazil
    Eur J Pharmacol 343:257-63. 1998
    ..In this study, we have investigated the in vivo (rat paw and skin oedema) and in vitro (mast cell degranulation) inflammatory effects caused by bothropstoxin-I and bothropstoxin-II...
  4. ncbi Neutrophil granules and secretory vesicles in inflammation
    Mikkel Faurschou
    The Granulocyte Research Laboratory, Department of Haematology, Rigshospitalet 4042, University of Copenhagen, 9 Blegdamsvej, DK 2100 Copenhagen OE, Denmark
    Microbes Infect 5:1317-27. 2003
    ..This review summarises current knowledge of granule biology and highlights the effects of neutrophil degranulation in the acute inflammatory response...
  5. ncbi Mast-cell degranulation induced by physical stimuli involves the activation of transient-receptor-potential channel TRPV2
    D Zhang
    JWG University Frankfurt, Institute for Biophysics, Frankfurt am Main, Germany
    Physiol Res 61:113-24. 2012
    ..We, therefore, suggest that TRPV2 plays a key role in mast-cell degranulation in response to mechanical, heat and red laser-light stimulation.
  6. pmc CD4+CD25+ regulatory T cells suppress mast cell degranulation and allergic responses through OX40-OX40L interaction
    Giorgia Gri
    Department of Biomedical Science and Technology, University of Udine, 33100 Udine, Italy
    Immunity 29:771-81. 2008
    ..The demonstrated crosstalk between Treg cells and MCs defines a previously unrecognized mechanism controlling MC degranulation. Loss of this interaction may contribute to the severity of allergic responses...
  7. ncbi Munc13-4 is essential for cytolytic granules fusion and is mutated in a form of familial hemophagocytic lymphohistiocytosis (FHL3)
    Jerome Feldmann
    INSERM U429, Hopital Necker Enfants Malades, 75743 Paris, France
    Cell 115:461-73. 2003
    ..Expressed tagged hMunc13-4 localizes with cytotoxic granules at the immunological synapse. HMunc13-4 is therefore essential for the priming step of cytolytic granules secretion preceding vesicle membrane fusion...
  8. ncbi Corticotropin-releasing hormone induces skin mast cell degranulation and increased vascular permeability, a possible explanation for its proinflammatory effects
    T C Theoharides
    Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Endocrinology 139:403-13. 1998
    ..Moreover, acute psychological stress induces CRH-dependent mast cell degranulation. Intradermal administration of rat/human CRH (0...
  9. pmc Transactivation of sphingosine-1-phosphate receptors by FcepsilonRI triggering is required for normal mast cell degranulation and chemotaxis
    Puneet S Jolly
    Department of Biochemistry, Virginia Commonwealth University Medical Center, Richmond 23298, USA
    J Exp Med 199:959-70. 2004
    ..Together, our results suggest that activation of SphKs and consequently S1PRs by FcepsilonRI triggering plays a crucial role in mast cell functions and might be involved in the movement of mast cells to sites of inflammation...
  10. pmc JNK1 controls mast cell degranulation and IL-1{beta} production in inflammatory arthritis
    Monica Guma
    Laboratory of Gene Regulation and Signal Transduction, Department of Pharmacology and Pathology, Division of Rheumatology, School of Medicine, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 107:22122-7. 2010
    ..Hence, JNK1 controls mast cell degranulation and FcγR-triggered IL-1β production, in addition to regulating cytokine and matrix metalloproteinase ..
  11. pmc Differential regulation of mast cell degranulation versus cytokine secretion by the actin regulatory proteins Coronin1a and Coronin1b
    Niko Föger
    Department of Immunology and Cell Biology, Research Center Borstel, 23845 Borstel, Germany
    J Exp Med 208:1777-87. 2011
    ..Thus, coronin proteins, and in turn the actin cytoskeleton, exhibit a functional dichotomy as differential regulators of degranulation versus cytokine secretion in MC biology...
  12. ncbi Fyn kinase initiates complementary signals required for IgE-dependent mast cell degranulation
    Valentino Parravicini
    Molecular Inflammation Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892 1820, USA
    Nat Immunol 3:741-8. 2002
    ..pathway that does not require Lyn kinase or the adapter LAT for its initiation, but is necessary for mast cell degranulation. Lyn-deficiency enhanced Fyn-dependent signals and degranulation, but inhibited the calcium response...
  13. ncbi IL-33 exacerbates autoantibody-induced arthritis
    Damo Xu
    Division of Immunology, Infection and Inflammation, Glasgow Biomedical Research Centre, University of Glasgow, 120 University Place, Glasgow G12 8TA, UK
    J Immunol 184:2620-6. 2010
    ..IL-33 also enhanced autoantibody-mediated mast cell degranulation in vitro and in synovial tissue in vivo...
  14. ncbi Functional analysis of human NK cells by flow cytometry
    Yenan T Bryceson
    Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Methods Mol Biol 612:335-52. 2010
    ..In addition to offering insight into the regulation of human NK cell responses, these techniques are applicable to the assessment of various clinical conditions, including the diagnosis of immunodeficiency syndromes...
  15. ncbi Essential role for the p110delta phosphoinositide 3-kinase in the allergic response
    Khaled Ali
    Ludwig Institute for Cancer Research, 91 Riding House Street, London W1W 7BS, UK
    Nature 431:1007-11. 2004
    ..Inactivation of p110delta protects mice against anaphylactic allergic responses. These results identify p110delta as a new target for therapeutic intervention in allergy and mast-cell-related pathologies...
  16. pmc Human mast cell degranulation and preformed TNF secretion require mitochondrial translocation to exocytosis sites: relevance to atopic dermatitis
    Bodi Zhang
    Molecular Immunopharmacology and Drug Discovery Laboratory, Department of Molecular Physiology and Pharmacology, Tufts University School of Medicine, Boston, MA 02111, USA
    J Allergy Clin Immunol 127:1522-31.e8. 2011
    ..They secrete many mediators, including preformed TNF, in response to allergic, neuropeptide, and environmental triggers. However, regulation of mast cell degranulation is not well understood.
  17. pmc Systemic anaphylaxis in the mouse can be mediated largely through IgG1 and Fc gammaRIII. Assessment of the cardiopulmonary changes, mast cell degranulation, and death associated with active or IgE- or IgG1-dependent passive anaphylaxis
    I Miyajima
    Department of Pathology, Beth Israel Deaconess Medical Center East and Harvard Medical School, Boston, Massachusetts 02215, USA
    J Clin Invest 99:901-14. 1997
    ....
  18. ncbi Human serum IgE-mediated mast cell degranulation shows poor correlation to allergen-specific IgE content
    F Marchand
    Unite d Immuno Allergie, Institut Pasteur, Paris, France
    Allergy 58:1037-43. 2003
    ..Although allergen-specific IgE content in serum can be determined immunochemically, little is known about the relationship between this parameter and the strength of the degranulation response upon allergen triggering...
  19. ncbi Comparison of primary human cytotoxic T-cell and natural killer cell responses reveal similar molecular requirements for lytic granule exocytosis but differences in cytokine production
    Samuel C C Chiang
    Centre for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Blood 121:1345-56. 2013
    ..in UNC13D, STX11, or STXBP2 associated with familial hemophagocytic lymphohistiocytosis, CTL and NK cell degranulation were similarly impaired...
  20. ncbi In situ measurement of degranulation as a biosensor based on RBL-2H3 mast cells
    Rose Mary Z G Naal
    Baker Laboratory, Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853 1301, USA
    Biosens Bioelectron 20:791-6. 2004
    ..We also demonstrate its utility in detecting inhibition of degranulation by a known pharmacologic inhibitor that blocks Syk tyrosine kinase activity critical for cell activation...
  21. ncbi IL-33 induces IL-13 production by mouse mast cells independently of IgE-FcepsilonRI signals
    Lien H Ho
    Department of Pathology, Stanford University School of Medicine, Stanford, California 94305 5324, USA
    J Leukoc Biol 82:1481-90. 2007
    ..By contrast, IL-33 neither induced nor enhanced mast cell degranulation. At 200 ng/ml, IL-33 prolonged mast cell survival in the absence of IgE and impaired survival in the ..
  22. pmc Interleukin-33 amplifies IgE synthesis and triggers mast cell degranulation via interleukin-4 in naïve mice
    M Komai-Koma
    Institute of Infection, Immunity and Inflammation, University of Glasgow, UK
    Allergy 67:1118-26. 2012
    ..IL-33 administration increases serum IgE in mice with unknown mechanism. We tested the hypothesis that IL-33 provides an antigen-independent stimulus for IgE production and mast cell degranulation.
  23. ncbi Lipopolysaccharide enhances FcεRI-mediated mast cell degranulation by increasing Ca2+ entry through store-operated Ca2+ channels: implications for lipopolysaccharide exacerbating allergic asthma
    Chengbin Yang
    State Key Laboratory of Respiratory Disease for Allergy, Shengzhen University, China
    Exp Physiol 97:1315-27. 2012
    ..This study investigated the effect of LPS on antigen-stimulated mast cell degranulation and the underlying mechanisms...
  24. pmc Preferential signaling and induction of allergy-promoting lymphokines upon weak stimulation of the high affinity IgE receptor on mast cells
    Claudia Gonzalez-Espinosa
    Molecular Inflammation Section, Molecular Immunology and Inflammation Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 197:1453-65. 2003
    Mast cell degranulation and de novo cytokine production is a consequence of antigen-aggregation of the immunoglobulin E (IgE)-occupied high affinity receptor for IgE (Fc epsilon RI)...
  25. pmc Mas-related gene X2 (MrgX2) is a novel G protein-coupled receptor for the antimicrobial peptide LL-37 in human mast cells: resistance to receptor phosphorylation, desensitization, and internalization
    Hariharan Subramanian
    Department of Pathology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6030, USA
    J Biol Chem 286:44739-49. 2011
    ..knockdown of the G protein-coupled receptor kinases (GRK2 and GRK3) had no effect on LL-37-induced mast cell degranulation. This study identified MrgX2 as a novel G protein-coupled receptor for the antibacterial peptide LL-37 and ..
  26. ncbi A current understanding of Fc epsilon RI-dependent mast cell activation
    Juan Rivera
    Molecular Inflammation Section, Molecular Immunology and Inflammation Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Allergy Asthma Rep 8:14-20. 2008
    ..the produced amount of phosphatidylinositol (3,4,5)-trisphosphate (PIP3), a key regulator of mast cell degranulation. Fyn and Lyn also activate sphingosine kinases (SphK), which generate sphingosine-1-phosphate (S1P), thus ..
  27. pmc Negative regulation of immunoglobulin E-dependent allergic responses by Lyn kinase
    Sandra Odom
    Molecular Inflammation Section, Molecular Immunology and Inflammation Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 199:1491-502. 2004
    ..Thus, lyn-/- mice and mast cells thereof show multiple allergy-associated traits, causing reconsideration of the possible efficacy in therapeutic targeting of Lyn in allergic disease...
  28. ncbi Molecular regulation of mast cell activation
    Juan Rivera
    Molecular Inflammation Section, Molecular Immunology and Inflammation Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, MD 20892 1820, USA
    J Allergy Clin Immunol 117:1214-25; quiz 1226. 2006
    ..The unifying theme is that the regulatory steps mentioned herein are required for promoting effective responses while protecting against unwanted inflammatory responses...
  29. ncbi In vitro activation of murine DRG neurons by CGRP-mediated mucosal mast cell degranulation
    F de Jonge
    Laboratory of Cell Biology and Histology, Department of Biomedical Sciences, University of Antwerp, Groenenborgerlaan 171, B 2020 Antwerp, Belgium
    Am J Physiol Gastrointest Liver Physiol 287:G178-91. 2004
    ..10(-7) M) to Fluo-4-loaded MMC induced a transient rise in [Ca(2+)](i) after a lag time, indicative of mast cell degranulation and/or secretion...
  30. pmc Interferon-gamma protects against chronic viral myocarditis by reducing mast cell degranulation, fibrosis, and the profibrotic cytokines transforming growth factor-beta 1, interleukin-1 beta, and interleukin-4 in the heart
    Delisa Fairweather
    Department of Pathology, Johns Hopkins Medical Institutions, 720 Rutland Ave, Baltimore, MD 21205, USA
    Am J Pathol 165:1883-94. 2004
    ....
  31. ncbi Cutting Edge: Transmembrane phosphoprotein Csk-binding protein/phosphoprotein associated with glycosphingolipid-enriched microdomains as a negative feedback regulator of mast cell signaling through the FcepsilonRI
    Hidenori Ohtake
    Division of Hygienic Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    J Immunol 168:2087-90. 2002
    ....
  32. ncbi Cutting edge: genetic variation influences Fc epsilonRI-induced mast cell activation and allergic responses
    Yumi Yamashita
    Molecular Inflammation Section, Molecular Immunology and Inflammation Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 179:740-3. 2007
    ..The findings demonstrate a genetic influence on the extent of a mast cell's response and identify Fyn kinase as a contributory determinant...
  33. ncbi Exogenous dendritic cell homing to draining lymph nodes can be boosted by mast cell degranulation
    Shu rong Ren
    Department of Immunology, Medical College of Qingdao University, Qingdao 266071, China
    Cell Immunol 263:204-11. 2010
    ..As Langerhans cell emigration was enhanced after skin mast cell degranulation, we investigated the effect of local mast cell activation on exogenous bone marrow-derived DCs (BM-DCs) ..
  34. pmc IgE, mast cells, basophils, and eosinophils
    Kelly D Stone
    Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 1881, USA
    J Allergy Clin Immunol 125:S73-80. 2010
    ..Mast cells, basophils, and eosinophils are central effector cells in allergic inflammation, as well as in innate and adaptive immunity. This review highlights what is known about these components and their roles in disease pathogenesis...
  35. pmc Involvement of Bruton's tyrosine kinase in FcepsilonRI-dependent mast cell degranulation and cytokine production
    D Hata
    Division of Allergy, La Jolla Institute for Allergy and Immunology, San Diego, California 92121, USA
    J Exp Med 187:1235-47. 1998
    ..Taken together, these results demonstrate an important role for Btk in the full expression of FcepsilonRI signal transduction in mast cells...
  36. ncbi Mast cells migrate, but do not degranulate, in response to fractalkine, a membrane-bound chemokine expressed constitutively in diverse cells of the skin
    E J Papadopoulos
    Dermatology Branch, National Cancer Institute, Bethesda, MD 20892 1908, USA
    Eur J Immunol 30:2355-61. 2000
    ..Thus, CX3CR1 is expressed by MC and effectively mediates chemotaxis without inducing degranulation. We propose that the constitutive expression of FK on certain cells in the skin may be a factor in the tissue-specific homing of MC...
  37. pmc Inhibitory effect of carotenoids on the degranulation of mast cells via suppression of antigen-induced aggregation of high affinity IgE receptors
    Shota Sakai
    Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    J Biol Chem 284:28172-9. 2009
    ....
  38. pmc Mechanisms of eosinophilia in the pathogenesis of hypereosinophilic disorders
    Steven J Ackerman
    Department of Biochemistry and Molecular Genetics M C 669, Molecular Biology Research Building, Rm 2074, 900 S Ashland Avenue, The University of Illinois at Chicago College of Medicine, Chicago, IL 60607, USA
    Immunol Allergy Clin North Am 27:357-75. 2007
    ....
  39. ncbi Dysregulated FcepsilonRI signaling and altered Fyn and SHIP activities in Lyn-deficient mast cells
    Valerie Hernandez-Hansen
    Department of Pathology and Cancer Research and Treatment Center, University of New Mexico School of Medicine, CRF 205, 2325 Camino de Salud, Albuquerque, NM 87131, USA
    J Immunol 173:100-12. 2004
    ..The unexpected absence of the transient receptor potential channel, Trpc4, from Lyn(-/-) BMMCs may additionally contribute to their altered signaling properties...
  40. pmc Canonical transient receptor potential 5 channel in conjunction with Orai1 and STIM1 allows Sr2+ entry, optimal influx of Ca2+, and degranulation in a rat mast cell line
    Hong Tao Ma
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 180:2233-9. 2008
    ..These and other observations suggest that the Sr(2+)-permeable TRPC5 associates with STIM1 and Orai1 in a stoichiometric manner to enhance entry of Ca(2+) to generate a signal for degranulation...
  41. ncbi Essential role for Gab2 in the allergic response
    H Gu
    Cancer Biology Program, Division of Hematology and Oncology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    Nature 412:186-90. 2001
    ..Gab2 and/or its associated signalling molecules may be new targets for developing drugs to treat allergy...
  42. pmc Expansion of a unique CD57⁺NKG2Chi natural killer cell subset during acute human cytomegalovirus infection
    Sandra Lopez-Verges
    Department of Microbiology and Immunology and Cancer Research Institute, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 108:14725-32. 2011
    ..During acute CMV infection, the NKG2C(+) NK cells proliferated, became NKG2C(hi), and finally acquired CD57. Thus, we propose that CD57 might provide a marker of "memory" NK cells that have been expanded in response to infection...
  43. pmc Isoform-specific functions of phosphoinositide 3-kinases: p110 delta but not p110 gamma promotes optimal allergic responses in vivo
    Khaled Ali
    Centre for Cell Signalling, Institute of Cancer, Queen Mary University of London, Sir John Vane Research Centre, Charterhouse Square, London, United Kingdom
    J Immunol 180:2538-44. 2008
    ..inhibitors, confirms that both PI3K isoforms play an important role in FcepsilonRI-activated mast cell degranulation in vitro...
  44. pmc Deficiency of HIV-Gag-specific T cells in early childhood correlates with poor viral containment
    Sihong Huang
    Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02115, USA
    J Immunol 181:8103-11. 2008
    ..These cross-sectional data suggest that qualitative differences in the CD8 response, combined with a deficiency of HIV-specific CD4 cells, may contribute to the inability of young infants to limit replication of HIV...
  45. ncbi Detection of T-cell degranulation: CD107a and b
    Michael R Betts
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Methods Cell Biol 75:497-512. 2004
  46. ncbi Phosphoinositide 3-kinase gamma is an essential amplifier of mast cell function
    Muriel Laffargue
    Institute of Biochemistry, Department of Medicine, University of Fribourg, Rue du Musee 5, CH 1700 Fribourg, Switzerland
    Immunity 16:441-51. 2002
    ..PI3Kgamma thus relays inflammatory signals through various G(i)-coupled receptors and is central to mast cell function...
  47. pmc Immediate cytotoxicity but not degranulation distinguishes effector and memory subsets of CD8+ T cells
    Petra Wolint
    Institute for Microbiology, Eidgenossische Technische Hochschule Zurich, Schmelzbergstrasse 7, 8092, Switzerland
    J Exp Med 199:925-36. 2004
    ..Furthermore, these results provide a potential mechanism by which central memory CD8+ T cell-mediated death of antigen-presenting cells within the lymph node is avoided...
  48. ncbi Allergy-associated FcRbeta is a molecular amplifier of IgE- and IgG-mediated in vivo responses
    D Dombrowicz
    Laboratory of Allergy and Immunology, Beth Israel Deaconess Medical Center, and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02215, USA
    Immunity 8:517-29. 1998
    ....
  49. ncbi Several distinct properties of the IgE repertoire determine effector cell degranulation in response to allergen challenge
    Lars Harder Christensen
    Department of Experimental Immunology, ALK ABELLO A S, Hørsholm, Denmark
    J Allergy Clin Immunol 122:298-304. 2008
    ....
  50. pmc Corticotropin-releasing hormone induces skin vascular permeability through a neurotensin-dependent process
    Jill Donelan
    Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, Tufts New England Medical Center, 136 Harrison Avenue, Boston, MA 02111, USA
    Proc Natl Acad Sci U S A 103:7759-64. 2006
    ..These findings suggest that CRH induces skin vascular permeability through NT acting on mast cells and that both peptides should be considered in the pathogenesis of skin disorders exacerbated by stress...
  51. ncbi Regional citrate anticoagulation reduces polymorphonuclear cell degranulation in critically ill patients treated with continuous venovenous hemofiltration
    Khajohn Tiranathanagul
    Division of Nephrology, Department of Medicine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok
    Ther Apher Dial 15:556-64. 2011
    ..the potentially additive benefit effect of regional citrate anticoagulation (RCA) on polymorphonuclear (PMN) cell degranulation of myeloperoxidase (MPO) and cytokines production in patients with critically acute kidney injury (AKI) ..
  52. ncbi Age-induced reprogramming of mast cell degranulation
    MyTrang Nguyen
    Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA
    J Immunol 175:5701-7. 2005
    Mast cell degranulation can initiate an acute inflammatory response and contribute to the progression of chronic diseases...
  53. ncbi Dural mast cell degranulation is a putative mechanism for headache induced by PACAP-38
    Michael Baun
    Department of Neurology, Glostrup Hospital, University of Copenhagen, Glostrup, Denmark
    Cephalalgia 32:337-45. 2012
    ..In the present study we examine the hypothesis that PACAP-38 and its truncated version PACAP-27 but not VIP cause degranulation of mast cells in peritoneum and in dura mater...
  54. pmc Mast cell degranulation induced by chlorogenic acid
    Fang hua Huang
    National Drug Screening Center, China Pharmaceutical University, Nanjing, China
    Acta Pharmacol Sin 31:849-54. 2010
    ..To investigate the mechanism of chlorogenic acid (CA)-induced anaphylactoid reactions...
  55. pmc Protein kinase C-delta is a negative regulator of antigen-induced mast cell degranulation
    Michael Leitges
    Max Planck Institute for Experimental Endocrinology, 30625 Hannover, Germany
    Mol Cell Biol 22:3970-80. 2002
    Regulation of mast cell degranulation is dependent on the subtle interplay of cellular signaling proteins...
  56. pmc Mast cell degranulation mediates compound 48/80-induced hyperalgesia in mice
    Devavani Chatterjea
    Department of Biology, Macalester College, St Paul, MN 55105, USA
    Biochem Biophys Res Commun 425:237-43. 2012
    ..Basic secretagogue compound 48/80 induced plantar mast cell degranulation accompanied by thermal hyperalgesia, tissue edema, and neutrophil influx in the hindpaws of ND4 Swiss mice...
  57. pmc The cytokine interleukin-33 mediates anaphylactic shock
    Peter N Pushparaj
    Division of Immunology, Infection and Inflammation, Glasgow Biomedical Research Centre, University of Glasgow, Scotland, United Kingdom
    Proc Natl Acad Sci U S A 106:9773-8. 2009
    ..This report therefore reveals a hitherto unrecognized pathophysiological role of IL-33 and suggests that IL-33 may be a potential therapeutic target for anaphylaxis, a disease of considerable unmet medical need...
  58. ncbi IL-10 confers protection from mast cell degranulation in a mouse model of allergic conjunctivitis
    Virgilio G Bundoc
    Exp Eye Res 85:575-9. 2007
    ..This is the first in vivo rodent study which reports a novel role of IL-10 in stabilizing mast cells from degranulation by a secretagogue, by as of yet an unknown mechanism...
  59. ncbi Mast cell degranulation induced by lectins: effect on neutrophil recruitment
    A N Moreno
    Department of Cellular and Molecular Biology and Pathogenic Bioagents, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil
    Int Arch Allergy Immunol 132:221-30. 2003
    ..The present study supports the concept that mammalian lectins play a fundamental role in innate immunity...
  60. ncbi Thapsigargin-induced degranulation of mast cells is dependent on transient activation of phosphatidylinositol-3 kinase
    M Huber
    Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada
    J Immunol 165:124-33. 2000
    ..These studies have both identified an additional PI-3 kinase-dependent step within the mast cell degranulation process, possibly involving 3-phosphoinositide-dependent protein kinase-1 and a diacylglycerol-independent ..
  61. pmc Selective, α2β1 integrin-dependent secretion of il-6 by connective tissue mast cells
    Karissa D McCall-Culbreath
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tenn, USA
    J Innate Immun 3:459-70. 2011
    ....
  62. ncbi Kit and FcepsilonRI mediate unique and convergent signals for release of inflammatory mediators from human mast cells
    Thomas R Hundley
    National Institutes of Health, Bethesda, MD 20892 1760, USA
    Blood 104:2410-7. 2004
    ..The findings, in total, indicated that a combination of FcepsilonRI and Kit-mediated signals and transcriptional processes were required for optimal physiologic responses of human mast cells to antigen...
  63. ncbi Compound 48/80 causes oxidative stress in the adrenal gland of rats through mast cell degranulation
    Shingo Kaida
    Department of Anesthesiology, 2nd Teaching Hospital, Fujita Health University School of Medicine, Nagoya, Aichi 454 0012, Japan
    Free Radic Res 44:171-80. 2010
    ..5 h after C48/80 treatment, attenuated all these changes in the adrenal tissue. These results indicate that C48/80 causes oxidative stress in rat adrenal gland through mast cell degranulation.
  64. pmc p38MAPK, ERK and PI3K signaling pathways are involved in C5a-primed neutrophils for ANCA-mediated activation
    Jian Hao
    Renal Division, Key Laboratory of Renal Disease, Department of Medicine, Peking University First Hospital, Institute of Nephrology, Peking University, Ministry of Health of China, Beijing, China
    PLoS ONE 7:e38317. 2012
    ..The current study further investigated the signaling pathways of C5a-mediated priming of human neutrophils for ANCA-induced neutrophil activation...
  65. pmc Prostaglandin E2 activates EP2 receptors to inhibit human lung mast cell degranulation
    Linda J Kay
    Molecular Pharmacology, University of Sheffield, The Royal Hallamshire Hospital Floor M, Glossop Road, Sheffield, S Yorks S10 2JF
    Br J Pharmacol 147:707-13. 2006
    ..Collectively, these data suggest that PGE2 mediates effects on human lung mast cells by interacting with EP2 receptors...
  66. ncbi Syk and Lyn mediate distinct Syk phosphorylation events in FcɛRI-signal transduction: implications for regulation of IgE-mediated degranulation
    Michael P Sanderson
    Department of Pulmonary Diseases Research, Boehringer Ingelheim Pharma GmbH and Co KG, Biberach an der Riss, Germany
    Mol Immunol 48:171-8. 2010
    ..This study identifies the responsible kinases for the phosphorylation of key Syk tyrosines and the propagation of FcɛRI receptor mediated signal transduction in allergic responses...
  67. ncbi Mast cell degranulation in human periodontitis
    Shiguang Huang
    Department of Periodontology, Faculty of Dentistry, Jinan University School of Medicine, Guangzhou, China
    J Periodontol 84:248-55. 2013
    ..Limited attention has been given to the role of mast cells in periodontal diseases, and the effects of mast cell degranulation on the chronic stages of non-allergic inflammation, particularly in periodontitis, are not known...
  68. ncbi An antagonist of human protease activated receptor-2 attenuates PAR2 signaling, macrophage activation, mast cell degranulation, and collagen-induced arthritis in rats
    Rink Jan Lohman
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia
    FASEB J 26:2877-87. 2012
    ..changes (edema, pannus formation, synovial hyperplasia, collagen degradation, macrophage invasion, mast cell degranulation) compared to untreated arthritic controls...
  69. pmc Primary human airway epithelial cell-dependent inhibition of human lung mast cell degranulation
    Neil Martin
    Department of Infection, Immunity and Inflammation, Institute for Lung Health, University of Leicester, United Kingdom
    PLoS ONE 7:e43545. 2012
    ..The aim of this study was to examine the regulation of HLMC degranulation by primary AEC from healthy and asthmatic subjects, and investigate further the inhibitory mechanism...
  70. pmc Mountain cedar pollen induces IgE-independent mast cell degranulation, IL-4 production, and intracellular reactive oxygen species generation
    Shuichiro Endo
    Department of Otolaryngology Head and Neck Surgery, University of Yamanashi, Chuo, Yamanashi, Japan
    Cell Immunol 271:488-95. 2011
    ..These mechanisms have the potential for enhancing the allergenic potency of pollens...
  71. pmc E-prostanoid 2 receptors dampen mast cell degranulation via cAMP/PKA-mediated suppression of IgE-dependent signaling
    Mariona Serra-Pagès
    Laboratory of Molecular Immunogenetics, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA
    J Leukoc Biol 92:1155-65. 2012
    ....
  72. ncbi The intracellular granzyme B inhibitor, proteinase inhibitor 9, is up-regulated during accessory cell maturation and effector cell degranulation, and its overexpression enhances CTL potency
    Claire E Hirst
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia
    J Immunol 170:805-15. 2003
    ..In conclusion, the presence and subcellular localization of PI-9 in leukocytes and DCs are consistent with a protective role against ectopic or misdirected grB during an immune response...
  73. ncbi Deciphering the structure and function of FcεRI/mast cell axis in the regulation of allergy and anaphylaxis: a functional genomics paradigm
    Jayapal Manikandan
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    Cell Mol Life Sci 69:1917-29. 2012
    ..In this review, we discuss various cellular and molecular mechanisms triggered through FcεRI/mast cell axis in allergy and anaphylaxis with a special emphasis on the functional genomics paradigm...
  74. ncbi The quantity and duration of FcRgamma signals determine mast cell degranulation and survival
    Sho Yamasaki
    Department of Molecular Genetics H1, Graduate School of Medicine, Chiba University, Chiba, Japan
    Blood 103:3093-101. 2004
    Immunoglobulin E (IgE) bound to multivalent antigen (Ag) elicits mast cell degranulation but not survival; on the contrary, IgE in the absence of Ag (IgE(-Ag)) induces survival only but not degranulation...
  75. pmc Regulation of C3a receptor signaling in human mast cells by G protein coupled receptor kinases
    Qiang Guo
    Department of Pathology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 6:e22559. 2011
    ..However, the roles of these GRKs on the regulation of C3aR signaling and mediator release in human mast cells remain unknown...
  76. ncbi MAPK kinase 3 specifically regulates Fc epsilonRI-mediated IL-4 production by mast cells
    Adam J MacNeil
    Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia B3K 6R8, Canada
    J Immunol 187:3374-82. 2011
    ..Thus, mast cell MKK3 signaling contributes to IgE-dependent allergic inflammation and is a specific regulator of FcεRI-induced IL-4 production...
  77. ncbi Regulation of FcepsilonRI-mediated degranulation by an adaptor protein 3BP2 in rat basophilic leukemia RBL-2H3 cells
    Kiyonao Sada
    Division of Proteomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, 7 5 1 Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
    Blood 100:2138-44. 2002
    ..These data indicate that 3BP2 functions to positively regulate the FcepsilonRI-mediated tyrosine phosphorylation of PLC-gamma and thereby the signals leading to degranulation...
  78. ncbi Cause and effect relationship between myocardial mast cell number and matrix metalloproteinase activity
    Gregory L Brower
    Department of Anatomy, Physiology and Pharmacology, 106 Greene Hall, Auburn University, Auburn, AL 36849 5517, USA
    Am J Physiol Heart Circ Physiol 283:H518-25. 2002
    ..Cromolyn treatment prevented the increase in mast cell number and MMP activity. Thus it is concluded that cardiac mast cells play a major role in the regulation of MMP activity...
  79. ncbi Corticotropin-releasing hormone stimulates the in situ generation of mast cells from precursors in the human hair follicle mesenchyme
    Natsuho Ito
    Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu, Japan
    J Invest Dermatol 130:995-1004. 2010
    ..This highlights a previously unpublished finding about neuroendocrine control of human MC biology...
  80. ncbi Piecemeal degranulation as a general secretory mechanism?
    Enrico Crivellato
    Department of Medical and Morphological Research, University of Udine School of Medicine, Udine, Italy
    Anat Rec A Discov Mol Cell Evol Biol 274:778-84. 2003
    ..Further investigation will clarify whether PMD can be considered as a general mechanism for the slow release of bioactive stored materials by granulated secretory cells...
  81. ncbi Phosphoinositol 3-kinase-gamma mediates antineutrophil cytoplasmic autoantibody-induced glomerulonephritis
    Adrian Schreiber
    Department of Nephrology and Hypertension, Medical Faculty of the Charite, Franz Volhard Clinic, Max Delbruck Center for Molecular Medicine, Schwanebecker Chaussee 50, Berlin, Germany
    Kidney Int 77:118-28. 2010
    ..We found that PI3Kgamma plays a pivotal role in ANCA-induced NCGN and suggest that its specific inhibition may provide a novel treatment target...
  82. pmc Mast cell degranulation breaks peripheral tolerance
    V C de Vries
    Department of Microbiology and Immunology, Dartmouth Medical School and Norris Cotton Cancer Center, Lebanon, NH, USA
    Am J Transplant 9:2270-80. 2009
    ..Such a dramatic reversal of T(reg) function and tissue distribution by MC degranulation underscores how allergy may causes the transient breakdown of peripheral tolerance and episodes of acute T-cell inflammation...
  83. ncbi Small-interfering RNA-mediated identification and regulation of the ternary SNARE complex mediating RBL-2H3 mast cell degranulation
    J R Woska
    Department of Pharmaceutical Sciences, College of Pharmacy and Allied Health Professions, St John s University, Jamaica, NY 11439, USA
    Scand J Immunol 73:8-17. 2011
    ..The functional role(s) of specific SNARE family member complexes in mast cell degranulation has not been fully elucidated...
  84. pmc Integrin-dependent organization and bidirectional vesicular traffic at cytotoxic immune synapses
    Dongfang Liu
    Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
    Immunity 31:99-109. 2009
    ..Thus, cytotoxic immune synapses include a central region of bidirectional vesicular traffic, which is controlled by integrin signaling...
  85. ncbi Activation of murine lung mast cells by the adenosine A3 receptor
    Hongyan Zhong
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, Houston, TX 77030, USA
    J Immunol 171:338-45. 2003
    ..Nebulized IB-MECA directly induced lung mast cell degranulation in wild-type mice while having no effect in A(3) receptor knockout mice...
  86. ncbi Angiotensin II generation by mast cell alpha- and beta-chymases
    G H Caughey
    Cardiovascular Research Institute and Department of Medicine, University of California at San Francisco, Box 0911, San Francisco, CA 94143 0911, USA
    Biochim Biophys Acta 1480:245-57. 2000
    ....
  87. ncbi NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation
    Christine Tkaczyk
    Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 104:207-14. 2004
    ..NTAL, thus, appears to be an important link between the signaling pathways that are initiated by these receptors, culminating in mast cell degranulation.
  88. pmc Neuropeptides activate human mast cell degranulation and chemokine production
    Marianna Kulka
    National Research Council Canada, Room 432, 550 University Avenue, Charlottetown, PE, Canada
    Immunology 123:398-410. 2008
    ..These results are likely to have direct relevance to neuronally induced inflammatory diseases...
  89. ncbi Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulation
    Delphine C Malherbe
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Am J Physiol Lung Cell Mol Physiol 289:L856-66. 2005
    ..that multimerization of SP-D is required for maximal PSG aggregation and inhibition of PSG-induced mast cell degranulation. This study is the first to demonstrate that SP-D modulates IgE-mediated mast cell functions, which are ..
  90. ncbi P-Rex1 regulates neutrophil function
    Heidi C E Welch
    Inositide Laboratory, The Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, United Kingdom
    Curr Biol 15:1867-73. 2005
    ..In conclusion, P-Rex1 is an important regulator of neutrophil function by mediating a subset of Rac-dependent neutrophil responses. However, P-Rex1 is not an essential regulator of neutrophil chemotaxis and degranulation...
  91. ncbi The impact of bacterial infection on mast cell degranulation
    Jordan Wesolowski
    Department of Microbiology and Immunology, Thomas Jefferson University, 223 South 10th Street, Bluemle Life Science Building Room 750, Philadelphia, PA 19107, USA
    Immunol Res 51:215-26. 2011
    ..Here, we review different aspects of mast cell physiology and discuss hypotheses as to how bacteria may influence mast cell degranulation.
  92. pmc Phosphorylation of SNAP-23 by IkappaB kinase 2 regulates mast cell degranulation
    Kotaro Suzuki
    Laboratory of Genetics, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 134:485-95. 2008
    ..The findings suggest that IKK2 is a central player in allergic reactions...
  93. pmc Adenosine A2A receptor activation reduces infarct size in the isolated, perfused mouse heart by inhibiting resident cardiac mast cell degranulation
    Tyler H Rork
    Robert M Berne Cardiovascular Research Center, University of Virginia Health System, Charlottesville, VA, USA
    Am J Physiol Heart Circ Physiol 295:H1825-33. 2008
    ..hypothesized that ATL146e (a selective A2AAR agonist) might protect hearts in part by reducing cardiac mast cell degranulation. Hearts were isolated from five groups of congenic mice: A2AAR+/+ mice, A2AAR(-/-) mice, mast cell-deficient ..
  94. ncbi Effects of propolis from different areas on mast cell degranulation and identification of the effective components in propolis
    Rika Nakamura
    Division of Novel Foods and Immunochemistry, National Institute of Health Sciences, 1 18 1, Kami yoga, Setagaya Ku, Tokyo, 158 8501, Japan
    Int Immunopharmacol 10:1107-12. 2010
    ..Therefore, we compared the effects of water and ethanol extracts of propolis from Brazil and China on mast cell degranulation and cytokine production, thereby identifying effective components in propolis...
  95. ncbi Impaired tyrosine phosphorylation and Ca2+ mobilization, but not degranulation, in lyn-deficient bone marrow-derived mast cells
    H Nishizumi
    Department of Oncology, Institute of Medical Science, University of Tokyo, Japan
    J Immunol 158:2350-5. 1997
    ..Our data suggest that the activity of the other Src family kinases, such as c-Src, can complement the role of Lyn in inducing most, but not all, biologic and biochemical responses to Fc epsilon RI cross-linking...
  96. ncbi Differential involvement of Ca2+ and actin filament in leukocyte shape change
    Petra Schratl
    Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Graz, Austria
    Pharmacology 83:131-40. 2009
    ..This type of shape change does not require any functional actin filaments, but appears to be a consequence of degranulation and depends essentially on the activation of PLC and Ca2+ influx from the extracellular space...
  97. ncbi Allergen-specific antibody and cytokine responses, mast cell reactivity and intestinal permeability upon oral challenge of sensitized and tolerized mice
    C Perrier
    R and D Laboratory of the Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Clin Exp Allergy 40:153-62. 2010
    ..Food allergy has reached an epidemic level in westernized countries and although central mechanisms have been described, the variability associated with genetic diversity underscores the still unresolved complexity of these disorders...
  98. ncbi Activation of human mast cells by aggregated IgG through FcgammaRI: additive effects of C3a
    Michael R Woolhiser
    Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Clin Immunol 110:172-80. 2004
    ..3). Simultaneous activation of huMC via aggregated IgG and C3a led to additive degranulation. These data support a mechanism by which mast cells may contribute to the inflammatory component in fibrosis, vasculitis, and arthritis...
  99. ncbi SHIP's C-terminus is essential for its hydrolysis of PIP3 and inhibition of mast cell degranulation
    J E Damen
    Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada
    Blood 97:1343-51. 2001
    ..These results suggest that the C-terminus plays a critical role in enabling SHIP to hydrolyze PIP(3) and inhibit BMMC degranulation...
  100. ncbi Cutting edge: Differential regulation of chemoattractant receptor-induced degranulation and chemokine production by receptor phosphorylation
    J Ahamed
    Department of Pathology, School of Dental Medicine, University of Pennsylvania, 4010 Locust Street, Philadelphia, PA 19104, USA
    J Immunol 167:3559-63. 2001
    ..These data demonstrate that receptor phosphorylation, which provides a turn off signal for degranulation, is essential for CCL2 production...
  101. pmc Rcan1 negatively regulates Fc epsilonRI-mediated signaling and mast cell function
    Yong jun Yang
    Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia B3K 6R8, Canada
    J Exp Med 206:195-207. 2009
    ..Rcan1 deficiency also led to increased Fc epsilonRI-mediated mast cell degranulation and enhanced passive cutaneous anaphylaxis...

Research Grants64

  1. The Kallikrein/Kinin-Mast Cell Interaction in Volume Overload
    CHIH CHANG KEVIN WEI; Fiscal Year: 2013
    ..of kallikrein inhibition on in vivo LV function, ECM homeostasis, ISF BK and chymase activity, and mast cell degranulation in response to VO in the early and chronic time points...
  2. Dengue Infected Endothelial Cells Enhance Immune Cell Activation
    Erich R Mackow; Fiscal Year: 2013
    ..complement system producing C3a/C5a: chemotactic anaphylatoxins which trigger localized inflammation, mast cell degranulation and vascular permeability...
  3. Targeting Siglec-8/-F to treat eosinophil and mast cell related disorders
    Bruce S Bochner; Fiscal Year: 2013
    ..For allergic diseases, drugs that inhibit mast cell degranulation, reduce eosinophil numbers, or counteract their released mediators are useful therapies, but all remain ..
  4. Targeting Siglec-8/-F to treat eosinophil and mast cell related disorders
    Bruce S Bochner; Fiscal Year: 2012
    ..For allergic diseases, drugs that inhibit mast cell degranulation, reduce eosinophil numbers, or counteract their released mediators are useful therapies, but all remain ..
  5. Analysis of NKLAM: A Novel Gene Associated With Cellular Cytotoxicity
    Jacki Kornbluth; Fiscal Year: 2013
    ..Studies will also be performed to test the hypothesis that upon NK cell degranulation, NKLAM enters target cells and ubiquitinates proteins such as UCKL-1, enhancing target cell death...
  6. Histamine Receptor Norepinephrine in Cardiac Dysfunction
    Roberto Levi; Fiscal Year: 2013
    ..Roles of PKC2I, 7 and 8 in inhibiting mast cell degranulation will be studied in mast cell cultures...
  7. The role of stress and mast cells in a preclinical mouse model of migraine
    ANN CHRISTINE RADDANT; Fiscal Year: 2013
    ..We found that chemically induced mast cell degranulation preferentially induced light aversion in the nestin/hRAMP1 model...
  8. The Role of IgE in Autoimmune Skin Diseases
    Janet A Fairley; Fiscal Year: 2013
    ..BP IgE will be tested for its ability to trigger mast cell degranulation in the presence of antigen utilizing an RBL-SX cell model...
  9. CGRP-induced light aversion in a preclinical migraine model
    Andrew F Russo; Fiscal Year: 2013
    ..The mice also display enhanced light aversive behavior following chemically induced mast cell degranulation and a mild stress paradigm...
  10. Suppression of established IgE-mediated disease
    FRED DOUGLASS FINKELMAN; Fiscal Year: 2013
    ..with enhanced suppression of the IL-4 and IL-13 responses to Ag challenge, enhanced suppression of mast cell degranulation, or both;b) the extent of CD4+ T cell inhibition required to enhance anti-Fc5RI1 mAb suppression of ..
  11. Perinatal exposure to environmental estrogens and asthma pathogenesis
    Terumi Midoro Horiuti; Fiscal Year: 2010
    ..APCs) to support Th2 responses, shaping the responses of T cells to normal APCs, and/or enhancing mast cell degranulation and production of cytokines...
  12. Role of Chymase In The Post-Myocardial Infarction Heart
    Ahsan Husain; Fiscal Year: 2013
    ..In Part I of this Aim we will define the temporal relationship between mast cell degranulation, MMCP-4-dependent pro-MMP-2/9 activation, and LV dilatation in ACE-inhibitor treated post-MI hearts and ..
  13. The Role of ASICs in Migraine Pathophysiology
    Gregory O Dussor; Fiscal Year: 2013
    ..The hypothesis of this proposal is that decreased extracellular pH within the dura following mast-cell degranulation leads to activation of dural afferents via the opening of acid-sensing ion channels (ASICs)...
  14. Beyond allergy: Mast cells mediate brain-behavior-immune interactions.
    Katherine M Nautiyal; Fiscal Year: 2010
    ..Both genetic and pharmacological models (using mast cell-deficient mice and a drug that blocks mast cell degranulation, respectively) of mast cell loss-of-function show that a lack of mast cells or their products result in ..
  15. Exercise, histamine receptors, and vascular function
    Steven A Romero; Fiscal Year: 2012
    ..been identified include: 1) carnosine released or leaked from skeletal muscle 2) histamine release via mast cell degranulation and 3) de novo production of histamine...
  16. Activation of the Trigeminovascular Pathway
    Rami Burstein; Fiscal Year: 2011
    ..that were pretreated (prior to CSD) for depletion of mast cells (using compound 48/80) or inhibition of mast cell degranulation (using sodium cromoglycate)...
  17. Mechanism and pathophysiological significance of mast cell regulated exocytosis
    Roberto Adachi; Fiscal Year: 2013
    ..It is accepted that mast cell degranulation should be important in the early allergic response because histamine and serotonin are secreted through this ..
  18. Regulation of Allergic Asthma by TGF-beta-induced Modulation of mMCP-1 and mMCP-4
    APARNA BALA SUNDARAM; Fiscal Year: 2013
    ..Mast cell degranulation also plays a key role in exacerbating allergic asthma, and is the therapeutic target of both leukotriene ..
  19. Identification of bioactive marine natural products that inhibit mast cells impli
    ESTHER AMALIA GUZMAN; Fiscal Year: 2010
    ..materials) from the HBOI marine specimen collection to identify those with the ability to inhibit mast cell degranulation;and to 3) validate hits found through the screening assays by functional angiogenesis and migration in vitro ..
  20. Protective and Deleterious Roles of Regulated Exocytosis from Mast Cells
    Roberto Adachi; Fiscal Year: 2010
    ..to uncover the specifics of Munc18-2 involvement in exocytosis, and to study the participation of mast cell degranulation in immune responses...
  21. PAK1's role in eotaxin-mediated murine eosinophil migration
    Muithi M Mwanthi; Fiscal Year: 2012
    ..Through actin-binding effectors, PAK1 modulates the actin cytoskeleton in mast cell degranulation, and additionally we have shown that this isoform is expressed in eosinophils...
  22. PATHOGENESIS OF ALLERGIC DISEASES OF THE SKIN & LUNGS
    Allen Kaplan; Fiscal Year: 1992
    ..Factors(s) (HRF) which are cytokines released from lymphocytes and/or monocytes to cause basophils and mast cell degranulation and determine its relationship to histamine-releasing cytokines we have identified such as interleukin 3 and ..
  23. Identification Of Proteins Responsible For Peanut Allergenicity
    Fred Finkelman; Fiscal Year: 2009
    ..immune system are not required for PE-induced shock;5) PE acts synergistically with IgE-mediated mast cell degranulation to induce shock;and 6) PE acts through a C3-independent mechanism to induce TNF production and low level Th2 ..
  24. CORTICOTROPIN RELEASING HORMONE INDUCED DURA MAST CELL A
    Theoharis Theoharides; Fiscal Year: 2001
    ..Acute psychological stress by immobilization triggered dura mast cell degranulation and resulted in cerebrospinal fluid (CSF) increase of rat mast cell protease (RMCP) I...
  25. BIOCHEMISTRY OF MAST CELL SECRETORY GRANULE ENZYMES
    Lawrence Schwartz; Fiscal Year: 1999
    ..These enzymes are selectively concentrate in mast cells, are released during mast cell degranulation and are the principal focus of this proposed renewal...
  26. HISTAMINE, IL-1 & NITRIC OXIDE IN WERNICKE LESIONS
    PHILIP LANGLAIS; Fiscal Year: 2002
    ..e., swelling of endfoot processes, perivascular edema, and mast cell degranulation and the late changes, i.e., number of apoptotic and necrotic of neurons within the thalamus of TD rats...
  27. GOBLET CELL DYSFUNCTION IN ASTHMA
    John Fahy; Fiscal Year: 2002
    ..goblet cell hyperplasia is a pathologic feature of animal models of asthma, and allergen-induced goblet cell degranulation causes airway obstruction in sensitized animals, the role of the goblet cell in the pathogenesis of mucus ..
  28. INDIVIDUAL FACTORS IN NASAL IRRITANT SENSITIVITY
    Dennis Shusterman; Fiscal Year: 2002
    ..Finally, biochemical markers of mast cell degranulation (tryphase) and neuro-immune modulation (nerve growth factor) will be assayed in nasal lavage fluid pre- and ..
  29. Recombinant Fc receptor therapeutics for systemic lupus erythematosus
    Periasamy Selvaraj; Fiscal Year: 2010
    ..available to bind multivalent IC stably and block IC-induced tissue damage, neutrophil emigration, and mast cell degranulation in mice...
  30. Allergy Prevention By lgE Cytotoxic Peptide(ECP)Vaccine
    Swey Shen Chen; Fiscal Year: 2002
    ..neutralizing antibodies, including internal image-type of antibodies that potentially can cause mast cell degranulation. It is imperative to design alternative therapeutic modality based on active IgE immunization with higher ..
  31. ROLE OF NERVOUS SYSTEM MAST CELLS IN DEMYELINATION
    David Johnson; Fiscal Year: 1990
    ..cells with myelin components and with cultured glial cells will be examined to study the effects of mast cell degranulation on the myelin sheath and glial cells, and to see whether substances present in the area of a demyelinating ..
  32. Development of an anti-PSA IgE to treat prostate cancer
    Birgit Schultes; Fiscal Year: 2009
    ..will evaluate the ability of the IgE to mediate ADCC of LNCaP tumor cells by eosinophils and test for mast cell degranulation. More elaborate in vivo studies are planned for Phase II using a human IgE/human Fc receptor transgenic ..
  33. Cardiac Mast Cell: Role in Pathogenesis of Heart Failure
    JOSEPH JANICKI; Fiscal Year: 2007
    ..marked extracellular matrix (ECM) degradation and subsequent ventricular dilatation; 2) inhibition of mast cell degranulation prevents the adverse myocardial remodeling and significantly attenuates mortality due to CHF; 3) significant ..
  34. REACTIVE OXYGEN SPECIES AND HEMOGLOBIN-INDUCED INJURY
    ANN BALDWIN; Fiscal Year: 2003
    ..injection of Hb, is due to the production of ROS and is catalysed by free iron, and amplified by mast cell degranulation. The role of nitric oxide in this process will also be investigated...
  35. The Function of SWAP-70 in Mast Cells
    Rolf Jessberger; Fiscal Year: 2007
    ..Details of the signaling pathways that trigger mast cell degranulation need to be further defined...
  36. AUTONOMIC REGULATION OF RESPIRATORY MUSCLE & SECRETION
    Alan Leff; Fiscal Year: 1992
    ..elucidated the potential influence of the sympathetic and parasympathetic nervous systems in modulating mast cell degranulation and airway contractile responses during immune challenge...
  37. LPS-PEPTIDE INTERACTION IN BLADDER INFLAMMATION
    Ricardo Saban; Fiscal Year: 2007
    ..of Protease Activated- Receptors (PAR) as a signaling mechanism owing to tryptase release following mast cell degranulation. There are 6 specific aims in this proposal seeking to determine the role of PARs in the development of ..
  38. Ultrasound, Intestinal and Cardiac Function in Rats
    ANN BALDWIN; Fiscal Year: 2004
    ..oxygen species (ROS) will be assessed using a fluorescent probe; epithelial sloughing and mucosal mast cell degranulation by microscopy...
  39. IMMUNOMODULATION OF FOOD ALLERGY BY DNA BASED IMMUNITY
    Hugh Sampson; Fiscal Year: 2002
    ..of hypersensitivity, including symptom score, the levels of serum specific IgE, plasma histamine, and mast cell degranulation are established...
  40. IGC REGULATION OF IGE-MEDIATED BASOPHIL DEGRANULATION
    John Cambier; Fiscal Year: 2001
    ..Based on recent findings, we hypothesize that FceRI mediated basophil and mast cell degranulation is subject to potent negative regulation by co-ligation with a coexpressed receptor for IgG Fc, FcgRIIB, and,..
  41. HISTAMINE ADRENERGICS AND SKELETAL MUSCLE ARTERIES
    LAURA MALLOY; Fiscal Year: 1991
    ..Vascular tension development in response to electrical field stimulation, mast cell degranulation, and the application of specific agonist and antagonists to histamine and catecholamines will be measured in ..
  42. MECHANISMS OF NEUROGENIC INFLAMMATORY RESPONSE
    Jen Wei; Fiscal Year: 1993
    ..also toward the periphery, participating in local inflammatory processes including vasodilatation and mast cell degranulation. However, the exact nature of this interesting and important phenomenon has yet to be ascertained...
  43. Mast Cell-Induced Inflammation in Vascular Disease
    Kottarappat N Dileepan; Fiscal Year: 2010
    ..4) Determine whether mast cell degranulation will accelerate the progression of atherosclerosis in an apoE-/- mice infected with C...
  44. MAST CELL SIGNALING IN ASTHMA AND ALLERGIC INFLAMMATION
    DIANA MARQUARDT; Fiscal Year: 2001
    ..Biochemical and molecular biology techniques will be utilized to determine whether PI3 kinase regulates mast cell degranulation but not cytokine release or leukotriene production.
  45. AUTONOMIC NEURONS--MODULATION BY PEPTIDES AND MAST CELLS
    JEROME FIEKERS; Fiscal Year: 1991
    ..Extracellular and intracellular electrophysiological recordings will be made from both ganglia and mast cell degranulation assessed by morphological and biochemical measurements...
  46. CAPACITATIVE CA INFLUX BY THE PHOTORECEPTOR CHANNEL TRP
    H Hartzell; Fiscal Year: 2000
    ..secretion of insulin from pancreatic acinar cells in response to secretagogues, and stimulation of mast cell degranulation. Despite the importance of SOCCs, very little is known about their molecular structure or control mechanisms,..
  47. Drug Target Discovery in Mast Cells by GECKO Technology
    P Jackson; Fiscal Year: 2003
    ..and create a genome-wide GECKO library for identification of genes whose function is necessary for mast cell degranulation. The specific aims of this phase I program, therefore, are 1) to develop robust high-throughput assays to ..
  48. ADENOSINE RECEPTORS IN ALLERGIC INFLAMMATION
    Stephen Tilley; Fiscal Year: 2004
    ..receptors on immune cells and in murine lung, determine the receptor responsible for adenosine-induced mast cell degranulation, and determine the significance of activation of these receptors by adenosine to the overall pathogenesis of ..
  49. REGULATION OF NERVOUS SYSTEM MAST CELL ACTIVITY
    David Johnson; Fiscal Year: 1993
    ..preliminary findings of mast cell migration into the nervous system, and examine the effects of in situ mast cell degranulation on the myelin sheath. Finally, Dr...
  50. MAST CELLS AND PERINATAL PULMONARY VASCULAR TONE
    Bonnie Taylor; Fiscal Year: 1991
    ..The role of mast cell degranulation and release of vasoactive agents in mediating or potentiating postnatal pulmonary vasoconstriction seen ..
  51. SECRETORY FUNCTION OF PANCREATIC DUCT CELLS
    Toan Nguyen; Fiscal Year: 2003
    ..b) characterize the vectorial release of ATP release from PDEC (autocrine action) c) study the effect of mast cell degranulation, resulting in ATP release, on PDEC secretion (paracrine action).
  52. The Mechanism of IVIG Action in Pemphigus
    Zhi Liu; Fiscal Year: 2006
    ..The overall goal of this project is to study the mechanisms of WIG action in autoantibody-mediated diseases and develop novel therapies to replace current immunosuppressive treatments, which confer severe side effects. ..
  53. REGULATION OF SECRETION FROM HUMAN BASOPHILS
    Donald MacGlashan; Fiscal Year: 2009
    ..A population of subjects will be examined for this relationship. ..
  54. RECEPTOR AGGREGATION ON THE CELL SURFACE
    Richard Posner; Fiscal Year: 2006
    ..The studies in this project are health related bearing on allergic reactions as well as ligand receptor reactions in general. ..
  55. Cellular Immunity to BK Virus
    Simon Lacey; Fiscal Year: 2005
    ..Such information may be useful in guiding clinical management, and lay the foundation for possible future vaccines. ..
  56. Immunobiology of Chlamydia
    Raymond Johnson; Fiscal Year: 2004
    ..3) To determine the effect of adoptive transfer of mucosal CD8 and CD4 T lymphocytes on the natural course of MoPn genital tract infections. ..
  57. Criteria for Protective Immunity to BK virus in Kidney Transplant Recipients
    Simon Lacey; Fiscal Year: 2007
    ..Information from the proposed study may allow identification of patients at risk of progression from BKV reactivation to clinical BKV disease and guide therapeutic interventions. [unreadable] [unreadable] [unreadable]..
  58. Cellular Immunity to Chlamydua at the Epithelial Interface
    RAYMOND MORRIS JOHNSON; Fiscal Year: 2010
    ..Defining what protective T cells look like, and how they function, will be critical for designing and assessing future Chlamydia candidate vaccines. ..
  59. Mast Cells: Role in the Pathophysiology of Headache
    Dan Levy; Fiscal Year: 2007
    ..Based on our preliminary data we hypothesized that dural mast cell degranulation, release of mediators and subsequent leukocyte recruitment promote activation of intracranial dural ..
  60. PROTEIN TYROSINE KINASES AND MAST CELL ACTIVATION
    Toshiaki Kawakami; Fiscal Year: 2001
    ..Particularly, interactions of Btk with Src PTKs and ERK might suggest positive and negative, respectively, feedback mechanisms for FceRI induced mast cell activation. ..
  61. Src Family Kinase Regulation in Allergy
    Becky Vonakis; Fiscal Year: 2007
    ..Understanding the molecular details of the interaction of Src family kinases with FceRI may allow the development of drugs that will limit allergic reactions rather than mitigating symptoms. ..
  62. Mast cell regulation by PKC and Akt
    Toshiaki Kawakami; Fiscal Year: 2005
    ..Given the critical importance of PKC in degranulation and other activation events, these studies are also likely to provide an opportunity for novel therapeutic modalities aimed at allergic diseases. ..
  63. SPHINGOLIPID METABOLITE, SPHINGOSINE 1 PHOSPHATE
    Sarah Spiegel; Fiscal Year: 2002
    ..This proposal provides a new paradigm for dissecting the complex interplay between intra and extracellular actions of sphingosine-1-phosphate. ..
  64. NEUROTROPHINS AND AN ANIMAL MODEL OF FIBROMYALGIA
    ALICE LARSON; Fiscal Year: 2003
    ..If kainic acid activity proves to be a useful model of FMS, therapeutic options may be more readily developed for this disease. ..