exocytosis

Summary

Summary: Cellular release of material within membrane-limited vesicles by fusion of the vesicles with the CELL MEMBRANE.

Top Publications

  1. ncbi Multiple roles of calcium ions in the regulation of neurotransmitter release
    Erwin Neher
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Neuron 59:861-72. 2008
  2. ncbi Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins
    G Miesenbock
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 394:192-5. 1998
  3. ncbi Molecular machines governing exocytosis of synaptic vesicles
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Nature 490:201-7. 2012
  4. ncbi Rab proteins as membrane organizers
    M Zerial
    Max Planck Institute of Molecular Cell Biology and Genetics, c o EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Rev Mol Cell Biol 2:107-17. 2001
  5. pmc Reduced insulin exocytosis in human pancreatic β-cells with gene variants linked to type 2 diabetes
    Anders H Rosengren
    Lund University Diabetes Centre, Department of Clinical Sciences Malmo, Lund University, Malmo, Sweden
    Diabetes 61:1726-33. 2012
  6. pmc GLUT4 exocytosis
    Jacqueline Stöckli
    Diabetes and Obesity Program, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia
    J Cell Sci 124:4147-59. 2011
  7. pmc Nanodomain coupling between Ca²⁺ channels and sensors of exocytosis at fast mammalian synapses
    Emmanuel Eggermann
    IST Austria Institute of Science and Technology Austria, Am Campus 1, A 3400 Klosterneuburg, Austria
    Nat Rev Neurosci 13:7-21. 2012
  8. pmc Exorcising the exocyst complex
    Margaret R Heider
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Traffic 13:898-907. 2012
  9. ncbi Intravesicular localization and exocytosis of alpha-synuclein and its aggregates
    He Jin Lee
    The Parkinson s Institute, Sunnyvale, California 94089, USA
    J Neurosci 25:6016-24. 2005
  10. ncbi Glutamate exocytosis from astrocytes controls synaptic strength
    Pascal Jourdain
    Department of Cell Biology and Morphology, University of Lausanne, Rue du Bugnon 9, 1005 Lausanne, Switzerland
    Nat Neurosci 10:331-9. 2007

Detail Information

Publications365 found, 100 shown here

  1. ncbi Multiple roles of calcium ions in the regulation of neurotransmitter release
    Erwin Neher
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Neuron 59:861-72. 2008
    ..Such coupling is essential for rendering vesicles sensitive to short [Ca(2+)] transients, generated during action potentials...
  2. ncbi Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins
    G Miesenbock
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 394:192-5. 1998
    ..combinatorial mutagenesis, with the aim of exploiting the acidic pH inside secretory vesicles to monitor vesicle exocytosis and recycling...
  3. ncbi Molecular machines governing exocytosis of synaptic vesicles
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Nature 490:201-7. 2012
    Calcium-dependent exocytosis of synaptic vesicles mediates the release of neurotransmitters. Important proteins in this process have been identified such as the SNAREs, synaptotagmins, complexins, Munc18 and Munc13...
  4. ncbi Rab proteins as membrane organizers
    M Zerial
    Max Planck Institute of Molecular Cell Biology and Genetics, c o EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Rev Mol Cell Biol 2:107-17. 2001
    ..These molecules are highly compartmentalized in organelle membranes, making them excellent candidates for determining transport specificity and organelle identity...
  5. pmc Reduced insulin exocytosis in human pancreatic β-cells with gene variants linked to type 2 diabetes
    Anders H Rosengren
    Lund University Diabetes Centre, Department of Clinical Sciences Malmo, Lund University, Malmo, Sweden
    Diabetes 61:1726-33. 2012
    ..We assessed the impact of 14 disease susceptibility variants on measures of glucose sensing, exocytosis, and structure...
  6. pmc GLUT4 exocytosis
    Jacqueline Stöckli
    Diabetes and Obesity Program, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia
    J Cell Sci 124:4147-59. 2011
    ..for the existence of these specific vesicles, how they are sequestered inside the cell and how they undergo exocytosis in the presence of insulin...
  7. pmc Nanodomain coupling between Ca²⁺ channels and sensors of exocytosis at fast mammalian synapses
    Emmanuel Eggermann
    IST Austria Institute of Science and Technology Austria, Am Campus 1, A 3400 Klosterneuburg, Austria
    Nat Rev Neurosci 13:7-21. 2012
    The physical distance between presynaptic Ca(2+) channels and the Ca(2+) sensors that trigger exocytosis of neurotransmitter-containing vesicles is a key determinant of the signalling properties of synapses in the nervous system...
  8. pmc Exorcising the exocyst complex
    Margaret R Heider
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Traffic 13:898-907. 2012
    ..demonstrates that the exocyst is also important for additional trafficking steps and cellular processes beyond exocytosis, with links to development and disease...
  9. ncbi Intravesicular localization and exocytosis of alpha-synuclein and its aggregates
    He Jin Lee
    The Parkinson s Institute, Sunnyvale, California 94089, USA
    J Neurosci 25:6016-24. 2005
    ..synthesized alpha-syn is rapidly secreted from cells via unconventional, endoplasmic reticulum/Golgi-independent exocytosis. Consistent with this finding, we also demonstrate that a portion of cellular alpha-syn is present in the lumen ..
  10. ncbi Glutamate exocytosis from astrocytes controls synaptic strength
    Pascal Jourdain
    Department of Cell Biology and Morphology, University of Lausanne, Rue du Bugnon 9, 1005 Lausanne, Switzerland
    Nat Neurosci 10:331-9. 2007
    ..The modalities and physiological functions of glial release are poorly understood. Here we show that glutamate exocytosis from astrocytes of the rat hippocampal dentate molecular layer enhances synaptic strength at excitatory synapses ..
  11. pmc Calcium-dependent protein kinase 1 is an essential regulator of exocytosis in Toxoplasma
    Sebastian Lourido
    Department of Molecular Microbiology, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, Missouri 63110, USA
    Nature 465:359-62. 2010
    Calcium-regulated exocytosis is a ubiquitous process in eukaryotes, whereby secretory vesicles fuse with the plasma membrane and release their contents in response to an intracellular calcium surge...
  12. ncbi Activated platelets release two types of membrane vesicles: microvesicles by surface shedding and exosomes derived from exocytosis of multivesicular bodies and alpha-granules
    H F Heijnen
    Department of Hematology, University Hospital Utrecht, The Netherlands
    Blood 94:3791-9. 1999
    ..These observations and the selective presence of CD63 suggest that released platelet exosomes may have an extracellular function other than the procoagulant activity, attributed to platelet microvesicles...
  13. ncbi Insulin granule dynamics in pancreatic beta cells
    P Rorsman
    The Oxford Centre for Diabetes, Endocrinology and Metabolism, Churchill Hospital, Headington, Oxford, UK
    Diabetologia 46:1029-45. 2003
    ..have been confined to the appearance of insulin in the extracellular space and the cellular events preceding exocytosis have been inaccessible to more detailed analysis...
  14. ncbi Rab11 regulates exocytosis of recycling vesicles at the plasma membrane
    Senye Takahashi
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Sci 125:4049-57. 2012
    ..at perinuclear recycling endosomes, Rab11 is transported along microtubules to the cell periphery through association with recycling carriers, and directly regulates vesicle exocytosis at the plasma membrane in concert with the exocyst.
  15. pmc The exocyst complex in polarized exocytosis
    Bing He
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 6018, USA
    Curr Opin Cell Biol 21:537-42. 2009
    ..Here we review the recent advances in the field, focusing on the function of the exocyst in polarized exocytosis.
  16. ncbi Plasma membrane repair is mediated by Ca(2+)-regulated exocytosis of lysosomes
    A Reddy
    Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT 06520, USA
    Cell 106:157-69. 2001
    Plasma membrane wounds are repaired by a mechanism involving Ca(2+)-regulated exocytosis. Elevation in intracellular [Ca(2+)] triggers fusion of lysosomes with the plasma membrane, a process regulated by the lysosomal synaptotagmin ..
  17. pmc A DOC2 protein identified by mutational profiling is essential for apicomplexan parasite exocytosis
    Andrew Farrell
    Department of Biology, Boston College, Chestnut Hill, MA 02467, USA
    Science 335:218-21. 2012
    b>Exocytosis is essential to the lytic cycle of apicomplexan parasites and required for the pathogenesis of toxoplasmosis and malaria. DOC2 proteins recruit the membrane fusion machinery required for exocytosis in a Ca(2+)-dependent fashion...
  18. pmc Temporary increase in plasma membrane tension coordinates the activation of exocytosis and contraction during cell spreading
    Nils C Gauthier
    Department of Biological Sciences, Fairchild Building, Columbia University, New York, NY 10027, USA
    Proc Natl Acad Sci U S A 108:14467-72. 2011
    ..is depleted, there is a temporary increase in membrane tension by over twofold that is followed by activation of exocytosis and myosin contraction...
  19. ncbi Do astrocytes really exocytose neurotransmitters?
    Nicola B Hamilton
    Department of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, London, WC1E 6BT, UK
    Nat Rev Neurosci 11:227-38. 2010
    ..However, there is intense controversy about whether astrocytes can exocytose transmitters in vivo. Resolving this issue would considerably advance our understanding of brain function...
  20. pmc AMPA receptor incorporation into synapses during LTP: the role of lateral movement and exocytosis
    Hiroshi Makino
    Department of Neurosciences, Division of Neurobiology, University of California, San Diego, La Jolla, CA 92093, USA
    Neuron 64:381-90. 2009
    ..These exocytosed receptors likely serve to replenish the local extrasynaptic pool available for subsequent bouts of plasticity. These results clarify the role of intracellular and surface AMPARs during synaptic plasticity...
  21. ncbi Rab6, Rab8, and MICAL3 cooperate in controlling docking and fusion of exocytotic carriers
    Ilya Grigoriev
    Department of Cell Biology, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands
    Curr Biol 21:967-74. 2011
    ..Taken together, the results of our study illustrate cooperation of two Rab proteins in constitutive exocytosis and implicates a redox enzyme in this process.
  22. pmc Familial hemophagocytic lymphohistiocytosis type 5 (FHL-5) is caused by mutations in Munc18-2 and impaired binding to syntaxin 11
    Udo Zur Stadt
    Research Institute Children s Cancer Center, 20251 Hamburg, Germany
    Am J Hum Genet 85:482-92. 2009
    ..Activity of natural killer and cytotoxic T cells was markedly reduced or absent, as determined by CD107 degranulation. Our findings thus identify a key role for STXBP2 in lytic granule exocytosis.
  23. ncbi Munc13-4 is essential for cytolytic granules fusion and is mutated in a form of familial hemophagocytic lymphohistiocytosis (FHL3)
    Jerome Feldmann
    INSERM U429, Hopital Necker Enfants Malades, 75743 Paris, France
    Cell 115:461-73. 2003
    ..HMunc13-4 mutations were shown to cause FHL3. HMunc13-4 deficiency results in defective cytolytic granule exocytosis, despite polarization of the secretory granules and docking with the plasma membrane...
  24. pmc Transcriptional activation of lysosomal exocytosis promotes cellular clearance
    Diego L Medina
    Telethon Institute of Genetics and Medicine TIGEM, Via P Castellino 111, 80131 Naples, Italy
    Dev Cell 21:421-30. 2011
    Lysosomes are cellular organelles primarily involved in degradation and recycling processes. During lysosomal exocytosis, a Ca²⁺-regulated process, lysosomes are docked to the cell surface and fuse with the plasma membrane (PM), ..
  25. pmc Exocyst function is regulated by effector phosphorylation
    Xiao Wei Chen
    Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA
    Nat Cell Biol 13:580-8. 2011
    ..vesicles, and binds to the exocyst after activation by insulin, but must then disengage to ensure continuous exocytosis. Here we report that, after recognition of the exocyst by activated RalA, disengagement occurs through ..
  26. ncbi Activation of RalA is required for insulin-stimulated Glut4 trafficking to the plasma membrane via the exocyst and the motor protein Myo1c
    Xiao Wei Chen
    Department of Molecular and Integrative Physiology, University of Michigan Medical Center, Ann Arbor, MI 48109, USA
    Dev Cell 13:391-404. 2007
    ..Thus, RalA serves two functions in insulin action: as a cargo receptor for the Myo1c motor, and as a signal for the unification of the exocyst to target Glut4 vesicles to the plasma membrane...
  27. ncbi The exocyst complex in exocytosis and cell migration
    Jianglan Liu
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Protoplasma 249:587-97. 2012
    b>Exocytosis is a fundamental membrane trafficking event in eukaryotic cells in which membrane proteins or lipids are incorporated into the plasma membrane and vesicle contents are secreted to the exterior of the cell...
  28. ncbi Distinct initial SNARE configurations underlying the diversity of exocytosis
    Haruo Kasai
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
    Physiol Rev 92:1915-64. 2012
    The dynamics of exocytosis are diverse and have been optimized for the functions of synapses and a wide variety of cell types...
  29. pmc Mechanisms of biphasic insulin-granule exocytosis - roles of the cytoskeleton, small GTPases and SNARE proteins
    Zhanxiang Wang
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    J Cell Sci 122:893-903. 2009
    ..granules to t-SNARE sites at the plasma membrane to enable the distal docking and fusion steps of insulin exocytosis. Nearly 40 years ago, the actin cytoskeleton was first recognized as a key mediator of biphasic insulin release, ..
  30. ncbi Actin coats and rings promote regulated exocytosis
    Thomas D Nightingale
    Medical Research Council Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK
    Trends Cell Biol 22:329-37. 2012
    ..Actin also has been extensively reported to be involved at several steps of exocytosis; however, it has typically been described as functioning either within the actin cortex or by providing actin ..
  31. ncbi The synaptic vesicle cycle
    Thomas C Sudhof
    Center for Basic Neuroscience, Department of Molecular Genetics, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9111, USA Thomas
    Annu Rev Neurosci 27:509-47. 2004
    Neurotransmitter release is mediated by exocytosis of synaptic vesicles at the presynaptic active zone of nerve terminals. To support rapid and repeated rounds of release, synaptic vesicles undergo a trafficking cycle...
  32. pmc Trypanosoma cruzi subverts the sphingomyelinase-mediated plasma membrane repair pathway for cell invasion
    Maria Cecilia Fernandes
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
    J Exp Med 208:909-21. 2011
    Upon host cell contact, the protozoan parasite Trypanosoma cruzi triggers cytosolic Ca(2+) transients that induce exocytosis of lysosomes, a process required for cell invasion...
  33. pmc Signaling, cytoskeletal and membrane mechanisms regulating GLUT4 exocytosis
    Nolan J Hoffman
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Centers for Diabetes Research, Membrane Biosciences, and Vascular Biology and Medicine, VanNuys Medical Science Building Room 308A, Indianapolis, IN 46202, USA
    Trends Endocrinol Metab 22:110-6. 2011
    ..New signaling and cytoskeletal mechanisms of insulin-stimulated GLUT4 exocytosis are of emerging interest, particularly those at or just beneath the plasma membrane...
  34. ncbi Secretory lysosomes
    Emma J Blott
    Sir William Dunn School of Pathology, Oxford University, South Parks Rd, Oxford OX1 3RE, UK
    Nat Rev Mol Cell Biol 3:122-31. 2002
    ..The importance of these organelles is highlighted by several genetic diseases, in which immune function and pigmentation--two processes that normally involve secretory lysosomes--are impaired...
  35. pmc Uncoupling the roles of synaptotagmin I during endo- and exocytosis of synaptic vesicles
    Jun Yao
    Howard Hughes Medical Institute and Department of Neuroscience, University of Wisconsin, Madison, Wisconsin, USA
    Nat Neurosci 15:243-9. 2012
    Synaptotagmin I (syt1) is required for normal rates of synaptic vesicle endo- and exocytosis. However, whether the kinetic defects observed during endocytosis in Syt1 knockout neurons are secondary to defective exocytosis or whether syt1 ..
  36. ncbi Alpha-neurexins couple Ca2+ channels to synaptic vesicle exocytosis
    Markus Missler
    Center for Basic Neuroscience, Department of Molecular Genetics, Dallas, Texas 75390 9111, USA
    Nature 423:939-48. 2003
    ..These data suggest that alpha-neurexins organize presynaptic terminals by functionally coupling Ca2+ channels to the presynaptic machinery...
  37. ncbi Otoferlin, defective in a human deafness form, is essential for exocytosis at the auditory ribbon synapse
    Isabelle Roux
    Inserm UMRS587, Unité de Génétique des Déficits Sensoriels, College de France, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France
    Cell 127:277-89. 2006
    ..However, the molecular mechanisms underlying IHC synaptic exocytosis are largely unknown...
  38. pmc Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p
    Charles Boyd
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Biol 167:889-901. 2004
    b>Exocytosis in the budding yeast Saccharomyces cerevisiae occurs at discrete domains of the plasma membrane. The protein complex that tethers incoming vesicles to sites of secretion is known as the exocyst...
  39. ncbi Calcium channel regulation and presynaptic plasticity
    William A Catterall
    Department of Pharmacology, University of Washington, Seattle, WA 98195 7280, USA
    Neuron 59:882-901. 2008
    ..We propose that presynaptic Ca(2+) channels serve as the regulatory node in a dynamic, multilayered signaling network that exerts short-term control of neurotransmission in response to synaptic activity...
  40. pmc The tip growth apparatus of Aspergillus nidulans
    Naimeh Taheri-Talesh
    Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
    Mol Biol Cell 19:1439-49. 2008
    ..We have investigated the central questions of the roles of cytoskeletal elements and of the precise sites of exocytosis and endocytosis at the growing hyphal tip by using the model fungus Aspergillus nidulans...
  41. pmc Exocytosis of acid sphingomyelinase by wounded cells promotes endocytosis and plasma membrane repair
    Christina Tam
    Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536, USA
    J Cell Biol 189:1027-38. 2010
    ..Earlier studies showed that plasma membrane repair requires Ca(2+)-dependent exocytosis of lysosomes and a rapid form of endocytosis that removes membrane lesions...
  42. ncbi Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
    Hadi Valadi
    Department of Internal Medicine, The Sahlgrenska Academy, Goteborg University, Sweden
    Nat Cell Biol 9:654-9. 2007
    ..In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA)...
  43. ncbi Calcium signaling and exocytosis in adrenal chromaffin cells
    Antonio G Garcia
    Instituto Teofilo Hernando, Departamento de Farmacologia y Terapeutica, and Servicio de Farmacología Clínica e Instituto Universitario de Investigación Gerontológica y Metabólica, Hospital Universitario de la Princesa, Madrid, Spain
    Physiol Rev 86:1093-131. 2006
    ..1 to approximately 50 microM, triggering CICR, mitochondrial Ca2+ uptake, and exocytosis at nearby secretory active sites...
  44. ncbi Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution
    R B Sutton
    The Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Nature 395:347-53. 1998
    ..These characteristics may be important for membrane fusion and for the binding of regulatory factors affecting neurotransmission...
  45. ncbi Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes
    J M Escola
    Department of Cell Biology, Utrecht University School of Medicine, AZU, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    J Biol Chem 273:20121-7. 1998
    ....
  46. pmc Rabs and the exocyst in ciliogenesis, tubulogenesis and beyond
    Amlan Das
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Trends Cell Biol 21:383-6. 2011
    The exocyst, an octameric protein complex mediating vesicle tethering at the plasma membrane for exocytosis, is a downstream effector of the Rab proteins Rab8 and Rab11, which are key regulators of membrane trafficking from the trans-..
  47. ncbi Fusion-related release of glutamate from astrocytes
    Qi Zhang
    Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Biol Chem 279:12724-33. 2004
    ..To determine whether calcium-dependent glutamate release is mediated by exocytosis, we expressed the SNARE motif of synaptobrevin II to prevent the formation of SNARE complexes, which reduces ..
  48. ncbi Spatial regulation of the exocyst complex by Rho1 GTPase
    W Guo
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520 8002, USA
    Nat Cell Biol 3:353-60. 2001
    ..The multiprotein exocyst complex is localized to sites of polarized exocytosis, and is required for vesicle targeting and docking at specific domains of the plasma membrane...
  49. pmc Essential role of Epac2/Rap1 signaling in regulation of insulin granule dynamics by cAMP
    Tadao Shibasaki
    Division of Cellular and Molecular Medicine, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, 7 5 1 Kusunoki cho, Chuo Ku, Kobe, Hyogo 650 0017, Japan
    Proc Natl Acad Sci U S A 104:19333-8. 2007
    cAMP is well known to regulate exocytosis in various secretory cells, but the precise mechanism of its action remains unknown...
  50. ncbi GLP-1 inhibits and adrenaline stimulates glucagon release by differential modulation of N- and L-type Ca2+ channel-dependent exocytosis
    Yang Z De Marinis
    Lund University Diabetes Centre, Department of Clinical Sciences, Clinical Research Centre, Lund University, SE20502 Malmö, Sweden
    Cell Metab 11:543-53. 2010
    ..is without much effect on alpha cell electrical activity but selectively inhibits N-type Ca(2+) channels and exocytosis. Adrenaline stimulates alpha cell electrical activity, increases [Ca(2+)](i), enhances L-type Ca(2+) channel ..
  51. ncbi Molecular mechanisms of biogenesis and exocytosis of cytotoxic granules
    Genevieve De Saint Basile
    Institut National de la Sante et de la Recherche Medicale INSERM, U768, Hopital Necker Enfants Malades, 149 rue de Sevres, 75015 Paris, France
    Nat Rev Immunol 10:568-79. 2010
    ..This has revealed a marked similarity between cytotoxic granule exocytosis at the immunological synapse and synaptic vesicle exocytosis at the neurological synapse...
  52. pmc Gelsolin associates with the N terminus of syntaxin 4 to regulate insulin granule exocytosis
    Michael A Kalwat
    Departments of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    Mol Endocrinol 26:128-41. 2012
    ..Taken together, these data support a mechanistic model for gelsolin's role in insulin exocytosis: gelsolin clamps unsolicited soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE)-regulated ..
  53. ncbi Astrocytes contain a vesicular compartment that is competent for regulated exocytosis of glutamate
    Paola Bezzi
    Department of Cell Biology and Morphology, University of Lausanne, and Cellular Imaging Facility UNIL CHUV Technological Development Unit, Switzerland
    Nat Neurosci 7:613-20. 2004
    ..Here we identify an astrocytic vesicular compartment that is competent for glutamate exocytosis. Using postembedding immunogold labeling of the rat hippocampus, we show that vesicular glutamate transporters (..
  54. ncbi Syntaxin-3 regulates newcomer insulin granule exocytosis and compound fusion in pancreatic beta cells
    D Zhu
    Department of Medicine, University of Toronto, Toronto, Ontario, Canada
    Diabetologia 56:359-69. 2013
    The molecular basis of the exocytosis of secretory insulin-containing granules (SGs) during biphasic glucose-stimulated insulin secretion (GSIS) from pancreatic beta cells remains unclear...
  55. pmc An exocyst complex functions in plant cell growth in Arabidopsis and tobacco
    Michal Hála
    Institute of Experimental Botany, Academy of Sciences of the Czech Republic, 165 02 Prague 6, Czech Republic
    Plant Cell 20:1330-45. 2008
    ..We conclude that the exocyst functions as a complex in plant cells, where it plays important roles in morphogenesis...
  56. ncbi Regulated ATP release from astrocytes through lysosome exocytosis
    ZhiJun Zhang
    Institute of Neuroscience and Key Laboratory of Neurobiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    Nat Cell Biol 9:945-53. 2007
    ..fluorescence microscopy (TIRFM), demonstrated directly that extracellular ATP or glutamate induced partial exocytosis of lysosomes, whereas an ischaemic insult with potassium cyanide induced both partial and full exocytosis of ..
  57. pmc Somatic exocytosis of serotonin mediated by L-type calcium channels in cultured leech neurones
    Citlali Trueta
    Departamento de Biofisica, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, D F, Mexico
    J Physiol 547:405-16. 2003
    We studied somatic exocytosis of serotonin and its mediation by L-type calcium (Ca2+) channels in cultured Retzius neurones of the leech...
  58. ncbi Starvation and rapamycin differentially regulate host cell lysosome exocytosis and invasion by Trypanosoma cruzi metacyclic forms
    Rafael Miyazawa Martins
    Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo, Rua Botucatu 862, Sao Paulo, Brazil
    Cell Microbiol 13:943-54. 2011
    ..Treatment of human epithelial HeLa cells with mTOR inhibitor rapamycin reduced lysosomal exocytosis and MT invasion...
  59. pmc Munc18-2 deficiency causes familial hemophagocytic lymphohistiocytosis type 5 and impairs cytotoxic granule exocytosis in patient NK cells
    Marjorie Côte
    Inserm U768, Hopital Necker Enfants Malades, Paris, France
    J Clin Invest 119:3765-73. 2009
    ..patients had strongly decreased STXBP2 protein expression, and NK cells exhibited impaired cytotoxic granule exocytosis, a defect that could be overcome by ectopic expression of wild-type STXBP2...
  60. pmc Mechanisms and function of dendritic exocytosis
    Matthew J Kennedy
    Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA
    Neuron 69:856-75. 2011
    Dendritic exocytosis is required for a broad array of neuronal functions including retrograde signaling, neurotransmitter release, synaptic plasticity, and establishment of neuronal morphology...
  61. ncbi Calcium triggers exocytosis from two types of organelles in a single astrocyte
    Tao Liu
    State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Molecular Medicine, Peking University, Beijing 100871, China
    J Neurosci 31:10593-601. 2011
    ..Here we set out to study vesicular exocytosis and glutamate release in mouse hippocampal astrocytes...
  62. pmc Role for the actomyosin complex in regulated exocytosis revealed by intravital microscopy
    Andrius Masedunskas
    Intracellular Membrane Trafficking Unit and Proteases and Tissue Remodeling Section, Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 4340, USA
    Proc Natl Acad Sci U S A 108:13552-7. 2011
    ..Here we used intravital microscopy in the salivary glands of live rodents to investigate regulated exocytosis, a fundamental process in all of the secretory organs...
  63. pmc Human mast cell degranulation and preformed TNF secretion require mitochondrial translocation to exocytosis sites: relevance to atopic dermatitis
    Bodi Zhang
    Molecular Immunopharmacology and Drug Discovery Laboratory, Department of Molecular Physiology and Pharmacology, Tufts University School of Medicine, Boston, MA 02111, USA
    J Allergy Clin Immunol 127:1522-31.e8. 2011
    ..They secrete many mediators, including preformed TNF, in response to allergic, neuropeptide, and environmental triggers. However, regulation of mast cell degranulation is not well understood...
  64. ncbi Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell surface projections regulates compound exocytosis in mast cells
    Z Guo
    Department of Cell Biology, University of Virgina Health Sciences Center, Charlottesville 22908, USA
    Cell 94:537-48. 1998
    For regulated secretion, mast cells and several other cell types utilize compound exocytosis, a combination of granule-plasma membrane and granule-granule fusions...
  65. ncbi Mitochondrial dysfunction, oxidative stress, regulation of exocytosis and their relevance to neurodegenerative diseases
    Damien J Keating
    Department of Human Physiology and Centre for Neuroscience, Flinders University, Adelaide, Australia
    J Neurochem 104:298-305. 2008
    ..with neurodegenerative diseases are now known to regulate either mitochondrial function, redox state, or the exocytosis of neurotransmitters...
  66. pmc Primary granule exocytosis in human neutrophils is regulated by Rac-dependent actin remodeling
    Troy Mitchell
    Dept of Cell Biology, Univ of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Am J Physiol Cell Physiol 295:C1354-65. 2008
    The actin cytoskeleton regulates exocytosis in all secretory cells. In neutrophils, Rac2 GTPase has been shown to control primary (azurophilic) granule exocytosis...
  67. pmc Identification of a vesicular nucleotide transporter
    Keisuke Sawada
    Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Okayama 700 8530, Japan
    Proc Natl Acad Sci U S A 105:5683-6. 2008
    ..Suppression of endogenous SLC17A9 expression in PC12 cells decreased exocytosis of ATP...
  68. pmc Exocytosis of post-Golgi vesicles is regulated by components of the endocytic machinery
    Jyoti K Jaiswal
    The Rockefeller University, 1230 York Avenue, Box 304, New York, NY 10065, USA
    Cell 137:1308-19. 2009
    ..b>Exocytosis of these vesicles is not calcium triggered...
  69. ncbi Ca(2+) and calmodulin initiate all forms of endocytosis during depolarization at a nerve terminal
    Xin Sheng Wu
    National Institute of Neurological Disorders and Stroke, Bethesda, Maryland, USA
    Nat Neurosci 12:1003-10. 2009
    ..Our findings provide a unifying mechanism for the initiation of various forms of endocytosis that are critical in maintaining exocytosis.
  70. ncbi Endocytosis, recycling, and regulated exocytosis of glucose transporter 4
    Kevin Foley
    Cell Biology Program, The Hospital for Sick Children, Toronto, Ontario M4G 1X8, Canada
    Biochemistry 50:3048-61. 2011
    ..GLUT4 traffic during its internalization from the membrane, its slow, constitutive recycling, and its regulated exocytosis in response to insulin...
  71. ncbi Multicolour imaging of post-Golgi sorting and trafficking in live cells
    P Keller
    Cell Biology/Biophysics Programme, European Molecular Biology Laboratory (EMBL, Meyerhofstrasse 1, D-69117 Heidelberg, Germany
    Nat Cell Biol 3:140-9. 2001
    ..Fusion with the plasma membrane was detected by total internal reflection microscopy and reveals differences between apical and basolateral carriers as well as new 'hot spots' for exocytosis.
  72. pmc Regulation of exocytosis by the exocyst subunit Sec6 and the SM protein Sec1
    Francesca Morgera
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Biol Cell 23:337-46. 2012
    ..complex termed the exocyst, which has been implicated in specific tethering of vesicles to sites of polarized exocytosis. The exocyst is directly involved in regulating soluble N-ethylmaleimide-sensitive factor (NSF) attachment ..
  73. pmc Neuronal somatic ATP release triggers neuron-satellite glial cell communication in dorsal root ganglia
    X Zhang
    Department of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX 77555 1069, USA
    Proc Natl Acad Sci U S A 104:9864-9. 2007
    ..FM1-43 photoconversion analysis further reveals that small clear vesicles participate in exocytosis. In addition, the released ATP activates P2X7 receptors in satellite cells that enwrap each DRG neuron and ..
  74. ncbi Glutamate receptor exocytosis and spine enlargement during chemically induced long-term potentiation
    Charles D Kopec
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Neurosci 26:2000-9. 2006
    ..We show that chemically induced long-term potentiation (chemLTP) drives robust exocytosis of AMPA receptors...
  75. ncbi A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis
    Shawn M Ferguson
    Howard Hughes Medical Institute, Kavli Institute for Neuroscience, Yale University School of Medicine, New Haven, CT 06510, USA
    Science 316:570-4. 2007
    ..Thus, dynamin 1-independent mechanisms can support limited synaptic vesicle endocytosis, but dynamin 1 is needed during high levels of neuronal activity...
  76. ncbi TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex
    Nicolas Blanchard
    Institut National de la Sante et de la Recherche Medicale, Unité 520, Institut Curie, Paris, France
    J Immunol 168:3235-41. 2002
    ....
  77. ncbi Mutations in Drosophila sec15 reveal a function in neuronal targeting for a subset of exocyst components
    Sunil Q Mehta
    Program in Developmental Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Neuron 46:219-32. 2005
    ..The data also show that functions of other exocyst components persist in the absence of sec15, suggesting that different exocyst components have separable functions...
  78. pmc The v-ATPase V0 subunit a1 is required for a late step in synaptic vesicle exocytosis in Drosophila
    P Robin Hiesinger
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 121:607-20. 2005
    ..Our data suggest that Vha100-1 functions downstream of SNAREs in synaptic vesicle fusion...
  79. ncbi Non-classical exocytosis of alpha-synuclein is sensitive to folding states and promoted under stress conditions
    Ara Jang
    Department of Anatomy, School of Medicine, Konkuk University, Seoul, Korea
    J Neurochem 113:1263-74. 2010
    ..This release is mediated by a non-classical, endoplasmic reticulum (ER)/Golgi-independent exocytosis, and stress-induced release coincides with increased translocation of alpha-synuclein into vesicles...
  80. ncbi Cytoskeletal control of vesicle transport and exocytosis in chromaffin cells
    J M Trifaro
    Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada
    Acta Physiol (Oxf) 192:165-72. 2008
    Chromaffin cell exocytosis is a fascinating interplay between secretory vesicles and cellular components. One of these components is the cytoskeleton and its associated regulatory proteins...
  81. pmc Secretory vesicle transport velocity in living cells depends on the myosin-V lever arm length
    Daniel H Schott
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    J Cell Biol 156:35-9. 2002
    ..Here we demonstrate in living, intact yeast that secretory vesicles move rapidly toward their site of exocytosis. The maximal speed varies linearly over a wide range of lever arm lengths genetically engineered into the myosin-..
  82. pmc Cell biology of Ca2+-triggered exocytosis
    Zhiping P Pang
    Department of Molecular and Cellular Physiology, Stanford University, 1050 Arastradero Rd, Palo Alto, CA 94304 5543, USA
    Curr Opin Cell Biol 22:496-505. 2010
    Ca(2+) triggers many forms of exocytosis in different types of eukaryotic cells, for example synaptic vesicle exocytosis in neurons, granule exocytosis in mast cells, and hormone exocytosis in endocrine cells...
  83. ncbi Phospholipase D1 production of phosphatidic acid at the plasma membrane promotes exocytosis of large dense-core granules at a late stage
    Maria Zeniou-Meyer
    Département Neurotransmission and Sécrétion Neuroendocrine, Institut des Neurosciences Cellulaires et Integratives, CNRS and Université Louis Pasteur, 5 rue Blaise Pascal, Strasbourg, France
    J Biol Chem 282:21746-57. 2007
    ..of lipids and lipid-modifying enzymes in various membrane trafficking processes, including calcium-regulated exocytosis of hormones and neurotransmitters...
  84. ncbi Identification of SNAREs involved in synaptotagmin VII-regulated lysosomal exocytosis
    Swathi K Rao
    Section of Microbial Pathogenesis and Department of Cell Biology, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06511, USA
    J Biol Chem 279:20471-9. 2004
    Ca2+-regulated exocytosis of lysosomes has been recognized recently as a ubiquitous process, important for the repair of plasma membrane wounds...
  85. pmc Gliotransmission: Exocytotic release from astrocytes
    Vladimir Parpura
    Department of Neurobiology, Center for Glial Biology in Medicine, Civitan International Research Center, Atomic Force Microscopy and Nanotechnology Laboratories, and Evelyn F McKnight Brain Institute, University of Alabama, Birmingham, USA
    Brain Res Rev 63:83-92. 2010
    ..It is becoming clear that astrocytes are endowed with elements that qualify them as cells communicating with neurons and other cells within the central nervous system by employing regulated exocytosis.
  86. pmc Bassoon and the synaptic ribbon organize Ca²+ channels and vesicles to add release sites and promote refilling
    Thomas Frank
    InnerEarLab, Department of Otolaryngology and Center for Molecular Physiology of the Brain, University of Göttingen Medical Center, 37099 Gottingen, Germany
    Neuron 68:724-38. 2010
    ..Ribbon absence reduced Ca²+ channel numbers at mutant and wild-type synapses. Fast and sustained exocytosis was reduced, notwithstanding normal coupling of the remaining Ca²+ channels to exocytosis...
  87. ncbi Imaging synaptic vesicle exocytosis and endocytosis with FM dyes
    Michael A Gaffield
    Neuroscience Program, University of Colorado Medical School, Aurora, Colorado 80045, USA
    Nat Protoc 1:2916-21. 2006
    ..Stimulation evokes exocytosis, and compensatory endocytosis that follows traps FM dye inside the retrieved vesicles...
  88. pmc Nitric oxide regulates exocytosis by S-nitrosylation of N-ethylmaleimide-sensitive factor
    Kenji Matsushita
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell 115:139-50. 2003
    ..We show that NO inhibits exocytosis of Weibel-Palade bodies, endothelial granules that mediate vascular inflammation and thrombosis, by regulating ..
  89. ncbi Dynamics and plasticity of Weibel-Palade bodies in endothelial cells
    Mariska G Rondaij
    Department of Plasma Proteins, AMC, University of Amsterdam, Amsterdam
    Arterioscler Thromb Vasc Biol 26:1002-7. 2006
    ..endothelin-1, and angiopoietin-2, is present within this subcellular organelle, implicating a role for WPB exocytosis in inflammation, hemostasis, regulation of vascular tone and angiogenesis...
  90. pmc Syntaxin clusters assemble reversibly at sites of secretory granules in live cells
    S Barg
    Department of Medical Cell Biology, Uppsala University, Uppsala 75123, Sweden
    Proc Natl Acad Sci U S A 107:20804-9. 2010
    Syntaxin resides in the plasma membrane, where it helps to catalyze membrane fusion during exocytosis. The protein also forms clusters in cell-free and granule-free plasma-membrane sheets...
  91. pmc Munc18c phosphorylation by the insulin receptor links cell signaling directly to SNARE exocytosis
    Jenna L Jewell
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    J Cell Biol 193:185-99. 2011
    ..small interfering ribonucleic acid reduction of Munc18c as a model of impaired insulin-stimulated GLUT4 vesicle exocytosis, we found that coordinate expression of Munc18c-wild type or select phosphomimetic Munc18c mutants, but not ..
  92. pmc Membrane proximal lysosomes are the major vesicles responsible for calcium-dependent exocytosis in nonsecretory cells
    Jyoti K Jaiswal
    Laboratory of Cellular Biophysics, Rockefeller University, New York, NY 10021, USA
    J Cell Biol 159:625-35. 2002
    Similar to its role in secretory cells, calcium triggers exocytosis in nonsecretory cells. This calcium-dependent exocytosis is essential for repair of membrane ruptures...
  93. ncbi Multigranular exocytosis of Weibel-Palade bodies in vascular endothelial cells
    Karine M Valentijn
    Department of Molecular Cell Biology, Section Electron Microscopy, Leiden University Medical Center, Leiden, The Netherlands
    Blood 116:1807-16. 2010
    Regulated exocytosis of Weibel-Palade bodies (WPBs) is a pivotal mechanism via which vascular endothelial cells initiate repair in response to injury and inflammation...
  94. pmc Intersectin-1L nucleotide exchange factor regulates secretory granule exocytosis by activating Cdc42
    Magali Malacombe
    Département Neurotransmission and Sécrétion Neuroendocrine, Institut des Neurosciences Cellulaires et Intégratives UMR 7168 LC2, Centre National de la Recherche Scientifique and Université Louis Pasteur, Strasbourg, France
    EMBO J 25:3494-503. 2006
    ..We previously reported that Cdc42 facilitates exocytosis in neuroendocrine cells by stimulating actin assembly at docking sites for secretory granules...
  95. ncbi Glucose-dependent docking and SNARE protein-mediated exocytosis in mouse pancreatic alpha-cell
    Sofia A Andersson
    Islet Cell Exocytosis, Department of Clinical Sciences Malmo, Lund University Diabetes Centre, Lund University, Malmo, Sweden
    Pflugers Arch 462:443-54. 2011
    ..Here, we aimed to investigate the temporal role of syntaxin 1A and SNAP-25 in mouse alpha-cell exocytosis. First, we used confocal imaging to investigate glucose dependency in the localisation of SNAP-25 and syntaxin ..
  96. pmc Exocytosis mechanisms underlying insulin release and glucose uptake: conserved roles for Munc18c and syntaxin 4
    Jenna L Jewell
    Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, USA
    Am J Physiol Regul Integr Comp Physiol 298:R517-31. 2010
    ..interventions, it will be vitally important to delineate the mechanisms governing these conserved SNARE-mediated exocytosis events...
  97. ncbi The ins and outs of IgE-dependent mast-cell exocytosis
    Ulrich Blank
    INSERM E 0225, Bichat Medical School, 16 rue Henri Huchard, BP 416, 75870 Cedex 18, France
    Trends Immunol 25:266-73. 2004
  98. ncbi Membrane fusion: SNAREs and regulation
    J Malsam
    Biochemistry Center, University Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
    Cell Mol Life Sci 65:2814-32. 2008
    ..The functional activity of some of these regulators determines the plasticity of regulated exocytosis.
  99. ncbi The ghost in the machine: small GTPases as spatial regulators of exocytosis
    Hao Wu
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Trends Cell Biol 18:397-404. 2008
    ..The localized activation of the exocyst by Rho GTPases is likely to have an important role in spatial regulation of exocytosis.
  100. pmc Rho GTPase regulation of exocytosis in yeast is independent of GTP hydrolysis and polarization of the exocyst complex
    Olivier Roumanie
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Biol 170:583-94. 2005
    ..This suggests that Rho3 and Cdc42 act to polarize exocytosis by activating the exocytic machinery at the membrane without the need to first recruit it to sites of polarized ..
  101. pmc Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis
    Michael Carnell
    Cancer Research UK Beatson Institute for Cancer Research, Bearsden, Glasgow G61 1BD, Scotland, UK
    J Cell Biol 193:831-9. 2011
    ..In this paper, we show that WASH coats mature Dictyostelium discoideum lysosomes and is essential for exocytosis of indigestible material...

Research Grants77

  1. Novel TIRF microscopy analyzing trafficking & signaling at the cell cortex
    Derek Toomre; Fiscal Year: 2009
    ..Consistent with the recovery act's goal, this funding will provide full-time summer employment for 4 undergraduate students and accelerate scientific achievement of the parent DP2 award...
  2. REGULATION AND FUNCTION OF STE20-RELATED PROTEIN KINASES
    Melanie H Cobb; Fiscal Year: 2012
    ..a dozen WNK1 targets that include proteins that directly activate ion transporters;regulate endocytosis, exocytosis and vesicular trafficking;and are components of signal transduction pathways essential for growth and ..
  3. Serotonin transporter-mediated regulation of neuroendocrine exocytosis
    KEVIN P CURRIE; Fiscal Year: 2013
    ..as a versatile neurosecretory model that enables detailed insight into the cellular and molecular mechanisms of exocytosis. Although 5HT is typically thought to act through cell surface receptors, recently it has been proposed to act ..
  4. Understanding thyroid hormone regulation of neurogenesis in the cochlea
    Mirna Mustapha; Fiscal Year: 2013
    ..function is compromised in Pit1dw mice with congenital, secondary hypothyroidism using physiological tests of exocytosis and measurement of both calcium and potassium currents, 2) Identify a novel set of thyroid hormone regulated ..
  5. Molecular control of differential peptide transmitter exocytosis.
    COREY B SMITH; Fiscal Year: 2013
    ..fusion, leading to the expansion of the exocytic fusion pore to maximize catecholamine release and facilitate exocytosis of the co-packaged adrenal peptide transmitters...
  6. Inflammation and Repair in Cardiac Ischemia-Reperfusion
    Lewis C Becker; Fiscal Year: 2011
    ..mediating the inflammatory response to myocardial ischemia-reperfusion injury, (4) the role of endothelial cell exocytosis in mediating inflammation after ischemia-reperfusion, and (5) the role of the transcription factor HIF-1 and its ..
  7. High-throughput multiplex microsphere screening for toxin protease inhibitors
    Steven W Graves; Fiscal Year: 2012
    ..protease kinetics at surfaces, and potentially as probes for the SNARE protein complex formation in the neuronal exocytosis pathway...
  8. FUNCTION OF THE RAS RELATED RAL PROTEINS
    LARRY FEIG; Fiscal Year: 2009
    ..including delivery of membrane proteins like E-cadherin to the basolateral plasma membrane of epithelial cells, exocytosis, apoptosis, cell migration and cell proliferation...
  9. Modulation of insulin and glucagon secretion by GPCR ligands
    TARA ASHLEY SCHWETZ; Fiscal Year: 2011
    ..Insight into ion channel function, Ca2+ signaling, and exocytosis in the presence and absence of NPY or SST will help elucidate the mechanism(s) by which these inhibitory ligands ..
  10. Molecular Imaging Probes for Reporting on Vascular Oxidative Response
    Alexei A Bogdanov; Fiscal Year: 2013
    ..Myeloperoxidase (MPO, one of the components released by PMNs via triggered exocytosis) is a unique catalytically active marker of inflammation sites, implicated in progression of vascular ..
  11. Mechanism of Neutrophil Activation
    Kenneth R McLeish; Fiscal Year: 2013
    ..Recent studies identified granule exocytosis and enhanced translocation of cytosolic components of the NADPH oxidase to the plasma membrane as possible ..
  12. 2011 Cell-Cell Fusion Gordon Research Conference and Seminar
    NANCY RYAN GRAY; Fiscal Year: 2011
    ..as well as the relationship between cell fusion and the larger fields of membrane fusion and fission during exocytosis, endocytosis, viral entry, and cytokinesis...
  13. TBI epileptogenesis: pathologic hippocampal L-glut synaptic plasticity
    John T Slevin; Fiscal Year: 2011
    ..Investigation of this putative relationship between aberrant glutamate neuronal exocytosis/resequestration and PTE will provide insight into potential mechanisms by which glutamate neurotransmission is ..
  14. Neuroprotective role of RAB3B in rodent models of Parkinson's disease
    Chee Yeun Chung; Fiscal Year: 2010
    ..RAB3B is localized to synaptic vesicles in neurons and is known to regulate neurotransmitter exocytosis by regulating synaptic vesicle trafficking processes including docking, fusion, and recycling at the synaptic ..
  15. Regulation of AKAP79 Postsynaptic Targeting and Signaling
    MARK L DELL'ACQUA; Fiscal Year: 2013
    ..Phosphorylation of GluA1 S845 by the kinase PKA promotes GluA1 endosomal recycling exocytosis leading to accumulation of receptors in the extrasynaptic plasma membrane where they are primed for synaptic ..
  16. CYTOPROTECTIVE EFFECTS OF INFLAMMATION MEDIATED MEMBRANE REPAIR
    DOUGLAS LOWELL MANN; Fiscal Year: 2013
    ..human cardiac myocytes generated from dermal fibroblasts from patients with dysferlinopathy have impaired exocytosis and increased cell death following hypoxia reoxygenation injury in vitro...
  17. PROTEINASE-ACTIVATED RECEPTORS/PERIPHERAL NERVOUS SYSTEM
    Nigel W Bunnett; Fiscal Year: 2010
    ..The mechanism by which sensitizing agents, such as PAR2, promote TRPV1 exocytosis, and by which desensitizing agents, such as capsaicin, promote TRPV1 endocytosis and degradation will be ..
  18. Lipid Signaling During Fertilization
    Bradley J Stith; Fiscal Year: 2010
    ..metabolism, membrane fusion during the sperm acrosome reaction, during sperm-egg fusion, during cortical granule exocytosis, and changes in microvilli...
  19. Role of nAChR stoichiometry in nicotine-induced upregulation
    CHRISTOPHER I RICHARDS; Fiscal Year: 2012
    ..endoplasmic reticulum (ER) varies with specific receptor subtypes and stoichiometries by observing variations in exocytosis and endocytosis activity of 1422...
  20. The role of the kimble gene in regulating craniofacial morphogenesis
    Daniel S Levic; Fiscal Year: 2013
    ..By investigating a zebrafish mutant with a malformed head skeleton, this project has identified impaired exocytosis as a potentially novel mechanism of chondrocyte apoptosis and cartilage degradation within the neurocranium and ..
  21. A High-throughput Screen of Lytic Granule Exocytosis
    Adam Zweifach; Fiscal Year: 2012
    ..Screening a small molecule library for compounds that inhibit lytic granule exocytosis would 1) serve as an assay for blockers of the known signals that control the process;2) reveal the presence of ..
  22. SYNAPTIC MECHANISMS OF GENERAL ANESTHETIC ACTION
    Hugh C Hemmings; Fiscal Year: 2013
    ..mechanisms;Aim 2-Determine the neurotransmitter-specific effects and mechanisms of volatile anesthetics on exocytosis in intact neurons to test the hypothesis that they differentially inhibit synaptic vesicle exocytosis by ..
  23. Elucidating the role of a-AP-2 in CFTR endocytosis and function in the intestine
    Anne M Collaco; Fiscal Year: 2013
    ..Conductance Regulator (CFTR) anion channels and its exocytic insertion back to the apical plasma membrane (exocytosis) regulate its surface expression and function in the intestine...
  24. MICROTUBULE-BASED TRANSPORT IN LACRIMAL GLAND FUNCTION
    Sarah F Hamm-Alvarez; Fiscal Year: 2012
    ..Within acinar cells, the proteins destined for apical secretion via regulated exocytosis and the proteins destined to function in the lysosomes (Lys) follow the same biosynthetic pathway through the ..
  25. Hepatitis C: E2 Molecular Mechanisms
    Martina Buck; Fiscal Year: 2013
    ..Trans Golgi network (TGN) sorting and exocytosis of the viral particle, through site-specific phosphorylations of <1 a subunit of the heterodimeric cargo ..
  26. PRESERVATION OF TIMING IN PLASTIC AUDITORY PATHWAYS
    HENRIQUE PRADO VON GERSDORFF; Fiscal Year: 2013
    ..glutamate release and simultaneously to measure membrane capacitance changes from the hair cell to assay the exocytosis of synaptic vesicles...
  27. Antigen Binding, Crosslinking and Transmembrane Signaling
    David A Holowka; Fiscal Year: 2013
    ..Ca2+, and we will investigate this spatio-temporally orchestrated process and its functional roles in mast cell exocytosis, cytokine production, and dynamic interactions with epithelial tissue...
  28. Pancreas Transcription Factors and Disease Model Systems
    Stephen F Konieczny; Fiscal Year: 2013
    ..cells that show defects in EGFR signaling and in the pathways controlling apical-basal polarity, regulated exocytosis, ER protein processing and intercellular communication...
  29. Junctional exocytosis and breakdown of the intestinal barrier in inflammation
    ANDREI IVANOVICH IVANOV; Fiscal Year: 2013
    ..of aging junctional components from the plasma membrane by endocytosis and delivery of new TJ/AJ proteins via exocytosis. A central innovative hypothesis of this proposal implies that intestinal inflammation interrupts a steady-state ..
  30. The Role of Bovine Sperm Acrosomal Membrane-Matrix Complex in Fertilization
    Subir K Nagdas; Fiscal Year: 2013
    ..determine the role of OMC32 and OMC45 polypetides in the calcium-dependent membrane fusion events of acrosomal exocytosis. Experiment #1c: To identify the Interaction of the known Members of the OMC Complex by Cross-Linking Studies: ..
  31. Real-time imaging of tPA fluorescent hybrids in hippocampal neurons
    Janis E Lochner; Fiscal Year: 2011
    ..The proposed experiments are directed at discerning if postsynaptic DCG exocytosis is spatially restricted to this syntaxin-4 enriched exocytic domain and if syntaxin-4 is required for DCG ..
  32. Antidote for botulism
    DAVID OWEN BEENHOUWER; Fiscal Year: 2010
    ..concentrations, displace active light chain, thereby promoting its degradation and re- constituting exocytosis. Preliminary Studies...
  33. MECHANISMS OF POLARIZED SECRETION BY BONE CELLS
    Steven L Teitelbaum; Fiscal Year: 2013
    ..Regulated secretion, also called exocytosis, requires cell polarity in which intracellular, cargo-containing vesicles are delivered, by organization of the ..
  34. RECEPTOR-MEDIATED TRANSPORT OF LYSOSOMAL ENZYMES
    William S Sly; Fiscal Year: 2013
    ..that mannose 6-phosphate (M6P) mediated transcytosis (endocytosis at the luminal membrane followed rapidly by exocytosis at the abluminal membrane without going through the lysosome) can be upregulated pharmacologically in adult ..
  35. Characterization of AQP2 interacting proteins and novel functions of AQP2
    HUA A LU; Fiscal Year: 2010
    ..of AQP2 requires direct and indirect protein-protein interactions during intracellular translocation, exocytosis as well as endocytosis...
  36. Synaptotagmin and C2-Domains: Structure and Function
    JOSE RIZO-REY; Fiscal Year: 2013
    ..studies devoted to compare the biochemical properties of other synaptotagmin isoforms involved in Ca2+- evoked exocytosis, and at deciphering the sequence determinants that underlie differences in these properties...
  37. Regulation and Neural Function of Atypical PKC
    Todd C Sacktor; Fiscal Year: 2011
    ..We will examine whether this potentiation is through increased exocytosis and/or decreased endocytosis of postsynaptic AMPARs and the function of this altered trafficking in memory ..
  38. Weibel - Palade Bodies - Sentinels of Acute Ischemia
    Michael S Goligorsky; Fiscal Year: 2013
    ..the chemical identities of stress (or SOS) signals discharged from the ischemic organ, some of which lead to 2) exocytosis of Weibel-Palade bodies and release of their constituents, such as angiopoietin-2, interleukin- 8 and von ..
  39. Optical studies of the cone photoreceptor synapse
    Richard H Kramer; Fiscal Year: 2013
    ..binds synaptic vesicles and is thought to deliver them to the plasma membrane where they undergo Ca2+-dependent exocytosis. Our first specific aim is to understand the mechanism of synaptic vesicle delivery by the ribbon, and to ..
  40. Phosphorylation-dependent regulation of epithelial sodium channel (ENaC) traffick
    Xiubin Liang; Fiscal Year: 2012
    ..the phosphorylation-dependent steps and protein interactions involved in apical membrane ENaC recycling and exocytosis have not been elucidated...
  41. Optical Study of Secretion in Mammalian Nerve Terminals
    BRIAN MATTHEW SALZBERG; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Secretion is one of the most ubiquitous of cellular processes. Regulated exocytosis is central to such diverse biological functions as information processing, reproduction, motility, temperature ..
  42. Electric Studies of Excitation, Secretion &Contraction
    Bertil Hille; Fiscal Year: 2013
    ..The hypothesis that serotonin is secreted from pinealocytes by quantal exocytosis whereas melatonin and N-acetyl serotonin are secreted by hydrophobic diffusion will also be tested...
  43. Excessive Lysosomal Exocytosis Triggers Pathogenic Mechanisms in Sialidosis Mice
    ALESSANDRA D'AZZO; Fiscal Year: 2013
    ..to dissect the role of NEU1 as a newly identified negative regulator of the physiological process of lysosomal exocytosis (LEX) and to test the hypothesis that excessive LEX, resulting from NEU1 loss of function, is the common ..
  44. Vesicular retrieval and reuse at CNS nerve terminals
    Richard W Tsien; Fiscal Year: 2012
    ..Following vesicle fusion and exocytosis of neurotransmitter, recycling of vesicular membrane is critical to allow transmission to continue during ..
  45. Intracellular Calcium Regulated NHE3 Endocytosis
    NICHOLAS CONSTANTINE ZACHOS; Fiscal Year: 2012
    ..its plasma membrane versus intracellular location as a result of alterations in the rates of endocytosis and/or exocytosis. In the small intestine, elevation of intracellular calcium ([Ca2+]i) by carbachol (mimics postprandial changes ..
  46. Structural and functional studies of botulinum neurotoxin
    Rongsheng Jin; Fiscal Year: 2013
    ..and therapeutic functions of BoNTs rely on a common mechanism to enter neurons, cleave proteins that mediate exocytosis of key neurotransmitters, and subsequently paralyze the affected muscles...
  47. Synergy of Host Defense Mechanisms in the Lung
    Arturo Casadevall; Fiscal Year: 2012
    ..cell cycle progression in macrophages;3) To identify the mechanism of action and outcome of macro-phagosome exocytosis in macrophages...
  48. Ca2+ and secretory dynamics in salivary acinar cells
    David I Yule; Fiscal Year: 2013
    ..In specific aim 2, experiments will be performed to elucidate why the exocytosis of protein is attenuated in SS models and if the function of ion channels necessary for fluid secretion is ..
  49. SECRETION FROM INDIVIDUAL VESICLES
    ROBERT MARK WIGHTMAN; Fiscal Year: 2012
    ..an applied potential sufficient to oxidize catecholamines) placed next to an isolated secretory cell can follow exocytosis as a series of current spikes composed of discrete packets of catecholamines detected by their electrooxidation...
  50. Role of Septins in Organelle Transfer
    Alan Michael Tartakoff; Fiscal Year: 2013
    ..as well as preliminary observations, we will determine whether the ZMC delimits a cortical zone within which exocytosis occurs...
  51. Neural Calcium Channels-Regulation and Function
    Diane Lipscombe; Fiscal Year: 2009
    ..that control channel modulation, channel degradation, and association with novel a target protein implicated in exocytosis. In the previous funding period of this grant we discovered an alternatively spiced exon in the C-terminus of ..
  52. Vesicular trafficking mechanisms regulating granulocyte function
    SERGIO DANIEL CATZ; Fiscal Year: 2013
    ..In neutrophils, vesicular trafficking is directly associated with the processes of exocytosis, phagocytosis, adhesion and chemotaxis;however, the molecular mechanisms that regulate mobilization of the ..
  53. Biophysical Characterization of Complexin C-terminal Domain Lipid Interactions
    David M Snead; Fiscal Year: 2013
    ..Cpx binds to the ternary SNARE complex and is known to directly regulate synaptic vesicle exocytosis. However, there has been great controversy regarding whether Cpx inhibits or stimulates synaptic vesicle fusion...
  54. Novel Regulators of Endothelial Exocytosis
    Charles J Lowenstein; Fiscal Year: 2013
    ..Vascular inflammation consists of several discrete stages, and the first stage is driven by endothelial cell exocytosis. The goal of this application is to identify novel cellular factors that regulate endothelial exocytosis...
  55. Macrophage Inflammasome Regulation
    MARK DAMIAN WEWERS; Fiscal Year: 2013
    ..g. lung fibroblasts or epithelium) in a highly concentrated packet where its secondary exocytosis can be controlled by target cell receptors that modulate local concentrations of ATP (a classical inflammasome ..
  56. Synaptic Changes by Stress in Prefrontal Cortex
    Zhen Yan; Fiscal Year: 2013
    ..small GTPases that function as specific regulators of vesicle trafficking at different stages of endocytosis/exocytosis. (3) To study the stress-induced changes in glutamate receptor trafficking and expression in PFC neurons...
  57. Regulation of auditory calcium channels
    Amy Lee; Fiscal Year: 2013
    ..Ca2+ channels mediate Ca2+ action potentials before the onset of hearing and Ca2+ signals that trigger exocytosis of neurotransmitter from IHCs onto auditory nerve afferents. These functions of Cav1...
  58. Mechanisms of fusion and transmitter release in neurosecretion
    Manfred Lindau; Fiscal Year: 2012
    ..and the molecular components forming the fusion pore are therefore central to understanding the mechanisms of exocytosis. The SNARE (Soluble NSF Attachment REceptor) complex, composed of the proteins synaptobrevin, syntaxin and SNAP-..
  59. Structural Mechanisms of Botulinum Neurotoxin Pathogenesis
    DANA BORDEN LACY; Fiscal Year: 2012
    ..They act by entering neurons and cleaving proteins that mediate the exocytosis of neurotransmitters...
  60. Role of SNARE Interactions in Central Synapse Function
    Ege T Kavalali; Fiscal Year: 2013
    ..This observation suggests a specific role for syb2 in ensuring faithful coupling between exocytosis and endocytosis...
  61. G PROTEIN STRUCTURE AND FUNCTION
    Heidi E Hamm; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Release of chemical transmitters by regulated exocytosis underlies many forms of intercellular communication, including hormone release and synaptic transmission...
  62. Functional Relevance of CFTR Trafficking in Vivo to Intestinal Disease
    Nadia A Ameen; Fiscal Year: 2013
    ..The mechanisms regulating Myo1a-dependent exocytosis of CFTR will now be examined...
  63. ADIPOCYTE PROTEIN SECRETION AND INSULIN ACTION
    Harvey Lodish; Fiscal Year: 2007
    ..specific aim, the PI will determine whether global or compartmentalized changes in cytosolic calcium affect exocytosis and secretion of insulin sensitive and insensitive secretory proteins...
  64. X-RAYS INDUCE EXOCYTOSIS OF ENDOTHELIAL P-SELECTIN
    Dennis Hallahan; Fiscal Year: 2002
    ..We have initiated our studies of the biological consequences of WPB exocytosis by use of the P-selectin knockout mouse. We observed two phenotypes in the P-selectin knockout mouse...
  65. HETEROTRIMERIC G PROTEINS AND EXOCYTOSIS
    BHANU JENA; Fiscal Year: 1999
    b>Exocytosis involves the delivery of secretory products packaged in membrane-bound vesicles to the extracellular space...
  66. REGULATION OF ENDOCYTOSIS IN NEUROENDOCRINE CELLS
    Cristina Artalejo; Fiscal Year: 2004
    ..Verbatim from Applicant's Description): Neurons and neuroendocrine cells release neurotransmitters/ hormones by exocytosis followed by recovery of vesicular membrane by endocytosis...
  67. Exocytosis and Coupled Endocytosis in Neuroendocrine Cells
    JOHN DAVID CASTLE; Fiscal Year: 2010
    The long-range objective is to understand the mechanisms involved in coupled exocytosis and endocytosis in neuroendocrine cells...
  68. EXOCYTIC INSERTION OF POTASSIUM CHANNELS IN MEMBRANES
    William Moran; Fiscal Year: 2001
    ..Alanine-induced increases in apical membrane exocytosis and capacitance suggest that the increase in plasma membrane Gk in Aplysia enterocytes, a model nutrient ..
  69. Role of Phosphatidic Acid in the Sperm Acrosome Reaction
    BRADLEY STITH; Fiscal Year: 2006
    ..AR is the exocytosis of the very large acrosome that is located at the forward edge of the head of the sperm...
  70. Secretion-associated dynamic of the plasma membrane
    Arun Anantharam; Fiscal Year: 2010
    ..namely, the nature of changes in plasma membrane morphology and biochemistry during the coupled processes of exocytosis and endocytosis...
  71. Stoichiometry and Architecture of the Vesicle Fusion Machine in PC-12 Cells
    JAMES WEISSHAAR; Fiscal Year: 2009
    ..In the key process of neuronal exocytosis, a voltage wave travels the length of the transmitting cell to open Ca2+ channels in the plasma membrane (PM) at ..
  72. ACROSOMAL MATRIX, EXOCYTOSIS AND MAMMALIAN FERTILIZATION
    GEORGE GERTON; Fiscal Year: 2007
    ..can become capacitated, bind to the zona pellucida (ZP) of an egg in a specific manner, and complete acrosomal exocytosis. Failure to carry out these functions results in infertility...
  73. METABOLIC CONTROL OF INSULIN EXOCYTOSIS
    Christopher Rhodes; Fiscal Year: 2005
    ..However, metabolic regulation of insulin exocytosis is only partly understood...
  74. Exocytosis and Vascular Inflammation
    Charles Lowenstein; Fiscal Year: 2007
    ..The early inflammatory response of endothelial cells includes the exocytosis of Weibel-Palade bodies, endothelial granules that contain yon Willebrand Factor (vWF) and P-selectin...
  75. REGULATION OF EXOCYTOSIS IN MAST CELLS
    J Castle; Fiscal Year: 2004
    ..products are stored in membrane-bounded granules within the cytoplasm, and their release occurs by compound exocytosis, a massive cascade of granule-plasma membrane and granule-granule fusions involving most if not all of the ..
  76. Analytical Techniques for Exocytosis
    Andrew Ewing; Fiscal Year: 2009
    ..apply analytical methods in extremely small environments to probe specific mechanisms that regulate and modulate exocytosis. A key aspect of this work will be to use amperometric measurements simultaneously with fluorescence imaging to ..
  77. REGULATION/INSULIN SECRETION BY PROTEIN PHOSPHORYLATION
    John Hutton; Fiscal Year: 2003
    ..though the fusion of pre-existing dense cored vesicles (termed DCVs or granules) with the plasma membrane and exocytosis of their contents...