phagocytosis

Summary

Summary: The engulfing of microorganisms, other cells, and foreign particles by phagocytic cells (PHAGOCYTES).

Top Publications

  1. ncbi Glucocorticoids induce protein S-dependent phagocytosis of apoptotic neutrophils by human macrophages
    Aisleen McColl
    Medical Research Council Centre for Inflammation Research, Queen s Medical Research Institute, Edinburgh, United Kingdom
    J Immunol 183:2167-75. 2009
  2. ncbi Neutrophil extracellular traps kill bacteria
    Volker Brinkmann
    Microscopy Core Facility, Max Planck Institute for Infection Biology, Schumannstrasse 21 22, 10117 Berlin, Germany
    Science 303:1532-5. 2004
  3. ncbi ATP mediates rapid microglial response to local brain injury in vivo
    Dimitrios Davalos
    Molecular Neurobiology Program, Department of Physiology and Neuroscience, New York University School of Medicine, 540 First Avenue, New York, New York 10016, USA
    Nat Neurosci 8:752-8. 2005
  4. pmc Microglia shape adult hippocampal neurogenesis through apoptosis-coupled phagocytosis
    Amanda Sierra
    Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA
    Cell Stem Cell 7:483-95. 2010
  5. pmc Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF
    V A Fadok
    Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Clin Invest 101:890-8. 1998
  6. pmc Nucleotides released by apoptotic cells act as a find-me signal to promote phagocytic clearance
    Michael R Elliott
    Beirne B Carter Center for Immunology Research, University of Virginia, Charlottesville, Virginia 22908, USA
    Nature 461:282-6. 2009
  7. pmc Clearance of apoptotic cells: implications in health and disease
    Michael R Elliott
    Center for Cell Clearance and the Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
    J Cell Biol 189:1059-70. 2010
  8. ncbi Aggregated alpha-synuclein activates microglia: a process leading to disease progression in Parkinson's disease
    Wei Zhang
    Neuropharmacology Section, Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences National Institutes of Health, Research Triangle Park, North Carolina, USA
    FASEB J 19:533-42. 2005
  9. ncbi Autoimmune disease and impaired uptake of apoptotic cells in MFG-E8-deficient mice
    Rikinari Hanayama
    Department of Genetics, Osaka University Medical School, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    Science 304:1147-50. 2004
  10. ncbi Mechanisms of phagocytosis in macrophages
    A Aderem
    Department of Immunology, University of Washington, Seattle 98195, USA
    Annu Rev Immunol 17:593-623. 1999

Detail Information

Publications380 found, 100 shown here

  1. ncbi Glucocorticoids induce protein S-dependent phagocytosis of apoptotic neutrophils by human macrophages
    Aisleen McColl
    Medical Research Council Centre for Inflammation Research, Queen s Medical Research Institute, Edinburgh, United Kingdom
    J Immunol 183:2167-75. 2009
    ..The mechanism underlying enhancement of macrophage capacity for phagocytosis of apoptotic cells by the powerful anti-inflammatory drugs of the glucocorticoid family has remained elusive...
  2. ncbi Neutrophil extracellular traps kill bacteria
    Volker Brinkmann
    Microscopy Core Facility, Max Planck Institute for Infection Biology, Schumannstrasse 21 22, 10117 Berlin, Germany
    Science 303:1532-5. 2004
    ..NETs appear to be a form of innate response that binds microorganisms, prevents them from spreading, and ensures a high local concentration of antimicrobial agents to degrade virulence factors and kill bacteria...
  3. ncbi ATP mediates rapid microglial response to local brain injury in vivo
    Dimitrios Davalos
    Molecular Neurobiology Program, Department of Physiology and Neuroscience, New York University School of Medicine, 540 First Avenue, New York, New York 10016, USA
    Nat Neurosci 8:752-8. 2005
    ..Thus, extracellular ATP regulates microglial branch dynamics in the intact brain, and its release from the damaged tissue and surrounding astrocytes mediates a rapid microglial response towards injury...
  4. pmc Microglia shape adult hippocampal neurogenesis through apoptosis-coupled phagocytosis
    Amanda Sierra
    Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA
    Cell Stem Cell 7:483-95. 2010
    ..These apoptotic newborn cells are rapidly cleared out through phagocytosis by unchallenged microglia present in the adult SGZ niche...
  5. pmc Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF
    V A Fadok
    Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Clin Invest 101:890-8. 1998
    ..b>Phagocytosis of apoptotic cells by macrophages has been suggested to be a quiet process that does not lead to production of ..
  6. pmc Nucleotides released by apoptotic cells act as a find-me signal to promote phagocytic clearance
    Michael R Elliott
    Beirne B Carter Center for Immunology Research, University of Virginia, Charlottesville, Virginia 22908, USA
    Nature 461:282-6. 2009
    ....
  7. pmc Clearance of apoptotic cells: implications in health and disease
    Michael R Elliott
    Center for Cell Clearance and the Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
    J Cell Biol 189:1059-70. 2010
    Recent advances in defining the molecular signaling pathways that regulate the phagocytosis of apoptotic cells have improved our understanding of this complex and evolutionarily conserved process...
  8. ncbi Aggregated alpha-synuclein activates microglia: a process leading to disease progression in Parkinson's disease
    Wei Zhang
    Neuropharmacology Section, Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences National Institutes of Health, Research Triangle Park, North Carolina, USA
    FASEB J 19:533-42. 2005
    ..Furthermore, microglial enhancement of alpha-synuclein-mediated neurotoxicity depended on phagocytosis of alpha-synuclein and activation of NADPH oxidase with production of reactive oxygen species...
  9. ncbi Autoimmune disease and impaired uptake of apoptotic cells in MFG-E8-deficient mice
    Rikinari Hanayama
    Department of Genetics, Osaka University Medical School, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    Science 304:1147-50. 2004
    ..These data demonstrate that MFG-E8 has a critical role in removing apoptotic B cells in the germinal centers and that its failure can lead to autoimmune diseases...
  10. ncbi Mechanisms of phagocytosis in macrophages
    A Aderem
    Department of Immunology, University of Washington, Seattle 98195, USA
    Annu Rev Immunol 17:593-623. 1999
    b>Phagocytosis of pathogens by macrophages initiates the innate immune response, which in turn orchestrates the adaptive response...
  11. ncbi Bone marrow-derived microglia play a critical role in restricting senile plaque formation in Alzheimer's disease
    Alain R Simard
    Laboratory of Molecular Endocrinology, CHUL Research Center and Department of Anatomy and Physiology, Laval University, 2705 Laurier Boul, Quebec G1V 4G2, Canada
    Neuron 49:489-502. 2006
    ..Therapeutic strategies aiming to improve their recruitment could potentially lead to a new powerful tool for the elimination of toxic senile plaques...
  12. ncbi Toll-like receptors stimulate human neutrophil function
    Fumitaka Hayashi
    Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, USA
    Blood 102:2660-9. 2003
    ..generation, and L-selectin shedding, while inhibiting chemotaxis to interleukin-8 (IL-8) and increasing phagocytosis of opsonized latex beads...
  13. ncbi Information processing during phagocytosis
    David M Underhill
    Inflammatory Bowel and Immunobiology Research Institute, Department of Biomedical Sciences, Cedars Sinai Medical Center, 8, 700 Beverly Boulevard, Los Angeles, California 90048, USA
    Nat Rev Immunol 12:492-502. 2012
    b>Phagocytosis - the process by which macrophages, dendritic cells and other myeloid phagocytes internalize diverse particulate targets - is a key mechanism of innate immunity...
  14. ncbi The retinal pigment epithelium in visual function
    Olaf Strauss
    Bereich Experimentelle Ophthalmologie, Klinik und Poliklinik fuer Augenheilkunde, Universitaetsklinikum Hamburg Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany
    Physiol Rev 85:845-81. 2005
    ..On the other hand, mutations in genes expressed in photoreceptors can lead to degenerations of the RPE. Thus both tissues can be regarded as a functional unit where both interacting partners depend on each other...
  15. ncbi Phylogenetic perspectives in innate immunity
    J A Hoffmann
    Institute of Molecular and Cellular Biology, CNRS, Strasbourg, 67084, France
    Science 284:1313-8. 1999
    ..In addition to its role in the early phase of defense, innate immunity in mammals appears to play a key role in stimulating the subsequent, clonal response of adaptive immunity...
  16. pmc Resolvin E1 and protectin D1 activate inflammation-resolution programmes
    Jan M Schwab
    Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 447:869-74. 2007
    ..Together, these results identify new active components for postexudate resolution traffic, and demonstrate that RvE1 and PD1 are potent agonists for resolution of inflamed tissues...
  17. pmc Clearance of apoptotic neurons without inflammation by microglial triggering receptor expressed on myeloid cells-2
    Kazuya Takahashi
    Neuroimmunology Unit, European Neuroscience Institute Göttingen, Germany
    J Exp Med 201:647-57. 2005
    ..extracellular signal-regulated kinase phosphorylation, and cytoskeleton reorganization and increased phagocytosis. Knockdown of TREM2 in microglia inhibited phagocytosis of apoptotic neurons and increased gene transcription ..
  18. pmc The identification of markers of macrophage differentiation in PMA-stimulated THP-1 cells and monocyte-derived macrophages
    Marc Daigneault
    Department of Infection and Immunity, Medical School, University of Sheffield, Sheffield, United Kingdom
    PLoS ONE 5:e8668. 2010
    ....
  19. pmc Microtubule-associated protein 1 light chain 3 alpha (LC3)-associated phagocytosis is required for the efficient clearance of dead cells
    Jennifer Martinez
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Proc Natl Acad Sci U S A 108:17396-401. 2011
    ..wherein the autophagy machinery is recruited to pathogen-containing phagosomes, termed MAPLC3A (LC3)-associated phagocytosis (LAP), which results in optimal degradation of the phagocytosed cargo...
  20. ncbi Identification of Tim4 as a phosphatidylserine receptor
    Masanori Miyanishi
    Department of Medical Chemistry, Graduate School of Medicine, Kyoto University, Yoshida, Sakyo ku, Kyoto 606 8501, Japan
    Nature 450:435-9. 2007
    ..These results indicate that Tim4 and Tim1 are phosphatidylserine receptors for the engulfment of apoptotic cells, and may also be involved in intercellular signalling in which exosomes are involved...
  21. ncbi Peripherally administered antibodies against amyloid beta-peptide enter the central nervous system and reduce pathology in a mouse model of Alzheimer disease
    F Bard
    Elan Pharmaceuticals, 800 Gateway Boulevard, South San Francisco, California 94080, USA
    Nat Med 6:916-9. 2000
    ..against amyloid beta-peptide triggered microglial cells to clear plaques through Fc receptor-mediated phagocytosis and subsequent peptide degradation...
  22. ncbi CED-1 is a transmembrane receptor that mediates cell corpse engulfment in C. elegans
    Z Zhou
    Howard Hughes Medical Institute, Department of Biology, Building 68, Room 425, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Cell 104:43-56. 2001
    ..We suggest that the ABC transporter CED-7 promotes cell corpse recognition by CED-1, possibly by exposing a phospholipid ligand on the surfaces of cell corpses...
  23. ncbi A blast from the past: clearance of apoptotic cells regulates immune responses
    John Savill
    MRC University of Edinburgh Centre for Inflammation Research, Department of Clinical and Surgical Sciences Internal Medicine, Royal Infirmary, Lauriston Place, Edinburgh EH3 9YW, UK
    Nat Rev Immunol 2:965-75. 2002
    ..Therefore, dead cells, which are a reflection of an organism's immediate past, can control its immunological future...
  24. pmc Macrophage activation switching: an asset for the resolution of inflammation
    F Porcheray
    Laboratoire de Neuro Immuno Virologie, Service de Neurovirologie UMR E1, Universite Paris Sud, Centre de Recherches du Service de Sante des Armees, IPSC, Fontenay aux Roses, France
    Clin Exp Immunol 142:481-9. 2005
    ..These findings may provide new insight into the inflammatory process as well as new fields of investigation for immunotherapy in the fields of chronic inflammatory diseases and cancer...
  25. ncbi The cell biology of phagocytosis
    Ronald S Flannagan
    Program in Cell Biology, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    Annu Rev Pathol 7:61-98. 2012
    Engulfment and destruction of invading microorganisms by phagocytosis are critical components of the innate immune response...
  26. pmc C1q and mannose binding lectin engagement of cell surface calreticulin and CD91 initiates macropinocytosis and uptake of apoptotic cells
    C A Ogden
    Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA
    J Exp Med 194:781-95. 2001
    ....
  27. ncbi Toll-like receptors in innate immunity
    Kiyoshi Takeda
    Department of Molecular Genetics, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Int Immunol 17:1-14. 2005
    ..The involvement of TLR-mediated pathways in autoimmune and inflammatory diseases has been proposed. Thus, TLR-mediated activation of innate immunity controls not only host defense against pathogens but also immune disorders...
  28. ncbi Neutrophil extracellular traps capture and kill Candida albicans yeast and hyphal forms
    Constantin F Urban
    Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Schumannstrasse 21 22, 10117 Berlin, Germany
    Cell Microbiol 8:668-76. 2006
    ..We demonstrate that NETs kill both yeast-form and hyphal cells, and that granule components mediate fungal killing. Taken together our data indicate that neutrophils trap and kill ascomycetous yeasts by forming NETs...
  29. ncbi Autophagy gene-dependent clearance of apoptotic cells during embryonic development
    Xueping Qu
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Cell 128:931-46. 2007
    ..We conclude that autophagy contributes to dead-cell clearance during PCD by a mechanism that likely involves the generation of energy-dependent engulfment signals...
  30. pmc Genome-wide analysis of the Drosophila immune response by using oligonucleotide microarrays
    E De Gregorio
    , Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette, France
    Proc Natl Acad Sci U S A 98:12590-5. 2001
    ..Many of these genes can be assigned to specific aspects of the immune response, including recognition, phagocytosis, coagulation, melanization, activation of NF-kappaB transcription factors, synthesis of antimicrobial peptides, ..
  31. ncbi Rho GTPases in cell biology
    Sandrine Etienne-Manneville
    MRC Laboratory for Molecular Cell Biology and Cell Biology Unit, Cancer Research UK Oncogene and Signal Transduction Group, University College London, Gower Street, London WC1E 6BT, UK
    Nature 420:629-35. 2002
    ..With spatial and temporal activation of multiple switches factored in, it is not surprising to find Rho GTPases having such a prominent role in eukaryotic cell biology...
  32. pmc A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages
    N Araki
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 135:1249-60. 1996
    ..studies, the inhibitors wortmannin and LY294002 inhibited fluid-phase pinocytosis and Fc receptor-mediated phagocytosis, but they had little effect on the receptor-mediated endocytosis of diI-labeled, acetylated, low density ..
  33. pmc CD14 and toll-like receptors 2 and 4 are required for fibrillar A{beta}-stimulated microglial activation
    Erin G Reed-Geaghan
    Department of Neurosciences, Center for Global Health and Diseases, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Neurosci 29:11982-92. 2009
    ..receptors could not initiate a Src-Vav-Rac signaling cascade leading to reactive oxygen species production and phagocytosis. The fAbeta-mediated activation of p38 MAPK also required CD14, TLR4, and TLR2...
  34. ncbi An immortalized cell line expresses properties of activated microglial cells
    V Bocchini
    Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy
    J Neurosci Res 31:616-21. 1992
    ..The BV-2 cell line thus represents a suitable model for in vitro studies of activated microglial cells...
  35. ncbi Apoptosis and necrosis: detection, discrimination and phagocytosis
    Dmitri V Krysko
    Molecular Signaling and Cell Death Unit, Department for Molecular Biomedical Research, VIB, 9052 Ghent, Belgium
    Methods 44:205-21. 2008
    ..A particular difficulty in defining necrosis is that in the absence of phagocytosis apoptotic cells become secondary necrotic cells with many morphological features of primary necrosis...
  36. pmc Contribution of Candida albicans cell wall components to recognition by and escape from murine macrophages
    C G J McKenzie
    College of Life Sciences and Medicine, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom
    Infect Immun 78:1650-8. 2010
    ..albicans innate immune interactions: phagocytosis by primary macrophages and macrophage cell lines, hyphal formation within macrophage phagosomes, and the ..
  37. pmc Microglia in the degenerating brain are capable of phagocytosis of beads and of apoptotic cells, but do not efficiently remove PrPSc, even upon LPS stimulation
    Martina M Hughes
    Trinity College Institute of Neuroscience and School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Republic of Ireland
    Glia 58:2017-30. 2010
    ..and urokinase plasminogen activator receptor (uPAR) but decreased, or did not alter, transcription of most phagocytosis-related genes examined and did not enhance clearance of deposited PrP(Sc)...
  38. pmc Immunobiology of the TAM receptors
    Greg Lemke
    Molecular Neurobiology Laboratory, The Salk Institute, 10010 N Torrey Pines Road, La Jolla, California 92037, USA
    Nat Rev Immunol 8:327-36. 2008
    ..They inhibit inflammation in dendritic cells and macrophages, promote the phagocytosis of apoptotic cells and membranous organelles, and stimulate the maturation of natural killer cells...
  39. ncbi Annexin-1 and peptide derivatives are released by apoptotic cells and stimulate phagocytosis of apoptotic neutrophils by macrophages
    Michael Scannell
    Diabetes Research Centre, School of Medicine and Medical Science, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland
    J Immunol 178:4595-605. 2007
    ..apoptotic human polymorphonuclear neutrophils (PMN), Jurkat T lymphocytes, and human mesangial cells promote phagocytosis of apoptotic PMN by Mphi and THP-1 cells differentiated to a Mphi-like phenotype...
  40. ncbi NOX2 controls phagosomal pH to regulate antigen processing during crosspresentation by dendritic cells
    Ariel Savina
    Institut Curie, INSERM U653, Immunité et Cancer, 26 Rue d Ulm, 75248 Paris, Cedex 05, France
    Cell 126:205-18. 2006
    ..Therefore, NOX2 plays a critical role in conferring DCs the ability to function as specialized phagocytes adapted to process antigens rather than kill pathogens...
  41. pmc TIM-1 and TIM-4 glycoproteins bind phosphatidylserine and mediate uptake of apoptotic cells
    Norimoto Kobayashi
    Department of Medical Oncology, Dana Farber Cancer Institute, 44 Binney Street, Harvard Medical School, Boston, MA 02115, USA
    Immunity 27:927-40. 2007
    ..TIM-1(+) kidney cells, and TIM-4- or TIM-1-transfected cells efficiently phagocytosed apoptotic cells, and phagocytosis could be blocked by TIM-4 or TIM-1 monoclonal antibodies...
  42. ncbi The glyoxylate cycle is required for fungal virulence
    M C Lorenz
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Massachusetts 02142, USA
    Nature 412:83-6. 2001
    ..In C. albicans, phagocytosis also upregulates the principal enzymes of the glyoxylate cycle, isocitrate lyase (ICL1) and malate synthase (..
  43. ncbi Human CD14 mediates recognition and phagocytosis of apoptotic cells
    A Devitt
    Department of Immunology, University of Birmingham Medical School, UK
    Nature 392:505-9. 1998
    ..Here we show that apoptotic cells interact with CD14, triggering phagocytosis of the apoptotic cells...
  44. pmc UDP acting at P2Y6 receptors is a mediator of microglial phagocytosis
    Schuichi Koizumi
    Division of Pharmacology, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya, Tokyo 158 8501, Japan
    Nature 446:1091-5. 2007
    ..and in vivo, a previously unrecognized mechanism underlying microglial chemotaxis; in contrast, microglial phagocytosis has received only limited attention...
  45. pmc Role of target geometry in phagocytosis
    Julie A Champion
    Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA
    Proc Natl Acad Sci U S A 103:4930-4. 2006
    b>Phagocytosis is a principal component of the body's innate immunity in which macrophages internalize targets in an actin-dependent manner. Targets vary widely in shape and size and include particles such as pathogens and senescent cells...
  46. pmc Resolvin E1 promotes phagocytosis-induced neutrophil apoptosis and accelerates resolution of pulmonary inflammation
    Driss El Kebir
    Research Center, Maisonneuve Rosemont Hospital, and Department of Pathology and Cell Biology, University of Montreal, Montreal, QC, Canada H1T 2M4
    Proc Natl Acad Sci U S A 109:14983-8. 2012
    ..Culture of human neutrophils with RvE1 accelerated apoptosis evoked by phagocytosis of opsonized Escherichia coli or yeast...
  47. ncbi Smoking alters alveolar macrophage recognition and phagocytic ability: implications in chronic obstructive pulmonary disease
    Sandra Hodge
    Department of Thoracic Medicine, Royal Adelaide Hospital and Lung Research Laboratory, Hanson Institute, Adelaide, South Australia
    Am J Respir Cell Mol Biol 37:748-55. 2007
    ..Our data indicates that a smoking-related reduction in AM phagocytic ability and expression of several important recognition molecules may be at least partially normalized in those subjects with COPD who have ceased smoking...
  48. ncbi The Toll-like receptor 2 is recruited to macrophage phagosomes and discriminates between pathogens
    D M Underhill
    Department of Immunology, University of Washington, Seattle 98195, USA
    Nature 401:811-5. 1999
    ..Thus, during the phagocytosis of pathogens, two classes of innate immune receptors cooperate to mediate host defence: phagocytic receptors, ..
  49. pmc The human macrophage mannose receptor directs Mycobacterium tuberculosis lipoarabinomannan-mediated phagosome biogenesis
    Peter B Kang
    Division of Infectious Diseases, Department of Internal Medicine, Center for Microbial Interface Biology, The Ohio State University, Columbus, OH 43210, USA
    J Exp Med 202:987-99. 2005
    ..The pattern recognition mannose receptor (MR) binds to the ManLAM mannose caps and mediates phagocytosis of bacilli by human macrophages...
  50. pmc Human atherosclerotic plaque alternative macrophages display low cholesterol handling but high phagocytosis because of distinct activities of the PPARγ and LXRα pathways
    Giulia Chinetti-Gbaguidi
    Univ Lille Nord de France, Lille, France
    Circ Res 108:985-95. 2011
    ..Macrophages are heterogeneous cells that adapt their response to environmental cytokines. Th1 cytokines promote monocyte differentiation into M1 macrophages, whereas Th2 cytokines trigger an "alternative" M2 phenotype...
  51. pmc Neuronal death induced by nanomolar amyloid β is mediated by primary phagocytosis of neurons by microglia
    Urte Neniskyte
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom
    J Biol Chem 286:39904-13. 2011
    ..exposed phosphatidylserine by adding annexin V or an antibody to phosphatidylserine or inhibiting microglial phagocytosis by adding either cytochalasin D (to block actin polymerization) or cyclo(RGDfV) (to block vitronectin receptors)..
  52. ncbi Neutrophil apoptosis and the resolution of infection
    Adam D Kennedy
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    Immunol Res 43:25-61. 2009
    ..b>Phagocytosis of bacteria typically accelerates neutrophil apoptosis, which ultimately promotes the resolution of infection...
  53. pmc The Streptococcus pneumoniae capsule inhibits complement activity and neutrophil phagocytosis by multiple mechanisms
    Catherine Hyams
    Centre for Respiratory Research, Department of Medicine, University College Medical School, Rayne Building, 5 University Street, London WC1E 6JF, United Kingdom
    Infect Immun 78:704-15. 2010
    The Streptococcus pneumoniae capsule is vital for virulence and may inhibit complement activity and phagocytosis. However, there are only limited data on the mechanisms by which the capsule affects complement and the consequences for S...
  54. ncbi M2 macrophages phagocytose rituximab-opsonized leukemic targets more efficiently than m1 cells in vitro
    Marzia Leidi
    Ospedali Riuniti di Bergamo, Italy
    J Immunol 182:4415-22. 2009
    ..Human macrophages, differentiated in vitro from peripheral blood monocytes, were used in binding and phagocytosis assays using B-chronic lymphocytic leukemia or lymphoma target cells opsonized with rituximab...
  55. pmc Infection regulates pro-resolving mediators that lower antibiotic requirements
    Nan Chiang
    Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 484:524-8. 2012
    ..With human polymorphonuclear neutrophils and macrophages, RvD1, RvD5 and PD1 each directly enhanced phagocytosis of E. coli, and RvD5 counter-regulated a panel of pro-inflammatory genes, including NF-κB and TNF-α...
  56. pmc Activation of the innate immune receptor Dectin-1 upon formation of a 'phagocytic synapse'
    Helen S Goodridge
    IBD and Immunobiology Research Institute, 8700 Beverly Boulevard, Cedars Sinai Medical Center, Los Angeles, California 90048, USA
    Nature 472:471-5. 2011
    ..that detects β-glucans in fungal cell walls and triggers direct cellular antimicrobial activity, including phagocytosis and production of reactive oxygen species (ROS)...
  57. ncbi Microglial phagocytosis of fibrillar beta-amyloid through a beta1 integrin-dependent mechanism
    Jessica Koenigsknecht
    Alzheimer Research Laboratory, Department of Neurosciences, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA
    J Neurosci 24:9838-46. 2004
    ..We report that exposure of microglia to fAbeta in vitro induces phagocytosis through mechanisms distinct from those used by the classical phagocytic receptors, the Ig receptors (FcRgammaI ..
  58. ncbi Phagocytosis-independent antimicrobial activity of mast cells by means of extracellular trap formation
    Maren von Köckritz-Blickwede
    Infection Immunology Research Group, Helmholtz Center for Infection Research, Braunschweig, Germany
    Blood 111:3070-80. 2008
    ..We took advantage of the ability of MCs to kill the human pathogen Streptococcus pyogenes by a phagocytosis-independent mechanism in order to characterize the extracellular antimicrobial activity of MCs...
  59. ncbi Tim-3 mediates phagocytosis of apoptotic cells and cross-presentation
    Masafumi Nakayama
    Department of Immunology, Biomedical Research Center, Juntendo University School of Medicine, Tokyo, Japan
    Blood 113:3821-30. 2009
    ..Furthermore, phagocytosis of apoptotic cells by CD8(+) DCs is inhibited by anti-Tim-3 mAb, resulting in a reduced cross-presentation of ..
  60. ncbi Annexin I is an endogenous ligand that mediates apoptotic cell engulfment
    Swathi Arur
    Center for Vascular Biology, Department of Physiology, University of Connecticut School of Medicine, 263 Farmington Avenue, Farmington, CT 06030, USA
    Dev Cell 4:587-98. 2003
    ..These results provide novel mechanistic insights into how apoptotic cells are removed and may explain a pathogenic mechanism of chronic inflammatory diseases where annexin I autoantibodies have been described...
  61. pmc Phagosome maturation: going through the acid test
    Jason M Kinchen
    Beirne B Carter Center for Immunology Research and the Department of Microbiology, University of Virginia, Charlottesville, Virginia 22902, USA
    Nat Rev Mol Cell Biol 9:781-95. 2008
    ..This suggests that a machinery that is distinct from receptor-mediated endocytosis is used in phagosome maturation...
  62. ncbi Molecular mechanisms of late apoptotic/necrotic cell clearance
    I K H Poon
    John Curtin School of Medical Research, Australian National University, Canberra, 2601, Australia
    Cell Death Differ 17:381-97. 2010
    b>Phagocytosis serves as one of the key processes involved in development, maintenance of tissue homeostasis, as well as in eliminating pathogens from an organism. Under normal physiological conditions, dying cells (e.g...
  63. ncbi Eater, a transmembrane protein mediating phagocytosis of bacterial pathogens in Drosophila
    Christine Kocks
    Laboratory of Developmental Immunology, Department of Pediatrics, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    Cell 123:335-46. 2005
    b>Phagocytosis is a complex, evolutionarily conserved process that plays a central role in host defense against infection. We have identified a predicted transmembrane protein, Eater, which is involved in phagocytosis in Drosophila...
  64. pmc Efficiency of salivary gland invasion by malaria sporozoites is controlled by rapid sporozoite destruction in the mosquito haemocoel
    JULIAN F HILLYER
    Center for Microbial and Plant Genomics and Department of Microbiology, University of Minnesota, 1500 Gortner Ave, Saint Paul, MN 55108, USA
    Int J Parasitol 37:673-81. 2007
    ..indicating that their failure to sequester large numbers of sporozoites is not due to an inability to engage in phagocytosis. These results reveal the operation of an efficient sporozoite-killing and degradation machinery within the ..
  65. pmc CD47 is upregulated on circulating hematopoietic stem cells and leukemia cells to avoid phagocytosis
    Siddhartha Jaiswal
    Ludwig Center at Stanford, Stanford Cancer Center, Department of Pathology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 138:271-85. 2009
    Macrophages clear pathogens and damaged or aged cells from the blood stream via phagocytosis. Cell-surface CD47 interacts with its receptor on macrophages, SIRPalpha, to inhibit phagocytosis of normal, healthy cells...
  66. ncbi Immunoregulatory functions of surfactant proteins
    Jo Rae Wright
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nat Rev Immunol 5:58-68. 2005
    ..In this article, I review the structure and functions of the surfactant proteins SP-A and SP-D in regulating host immune defence and in modulating inflammatory responses...
  67. ncbi Functions and molecular mechanisms of the CD47-SIRPalpha signalling pathway
    Takashi Matozaki
    Laboratory of Biosignal Sciences, Institute for Molecular and Cellular Regulation, Gunma University, 3 39 15 Showa Machi, Maebashi, Gunma, Japan
    Trends Cell Biol 19:72-80. 2009
    ..The interaction of SIRPalpha with CD47 is important for the regulation of migration and phagocytosis. Recent studies have implicated the CD47-SIRPalpha signalling pathway in immune homeostasis and in regulation ..
  68. ncbi High-yield isolation of murine microglia by mild trypsinization
    Josep Saura
    Department of Pharmacology and Toxicology, Institut d Investigacions Biomediques de Barcelona, IIBB CSIC, Barcelona, Spain
    Glia 44:183-9. 2003
    ..These almost pure microglial preparations can be kept in culture for weeks and show proliferation and phagocytosis. Treatment with macrophage colony-stimulating factor and lipopolysaccharide, alone or in the presence of ..
  69. pmc Debris clearance by microglia: an essential link between degeneration and regeneration
    H Neumann
    Neural Regeneration, Institute of Reconstructive Neurobiology, University Bonn and Hertie Foundation, Bonn, Germany
    Brain 132:288-95. 2009
    ..Thus, understanding the mechanism and functional significance of microglial-mediated clearance of tissue debris following injury may open up exciting new therapeutic avenues...
  70. pmc LAMP proteins are required for fusion of lysosomes with phagosomes
    Kassidy K Huynh
    Division of Cell Biology, The Hospital for Sick Children, Toronto, Canada
    EMBO J 26:313-24. 2007
    ..Instead, we reconstituted phagocytosis in murine embryonic fibroblasts (MEFs) by transfection of FcgammaIIA receptors...
  71. ncbi Calreticulin exposure dictates the immunogenicity of cancer cell death
    Michel Obeid
    INSERM U848, 39 rue Camille Desmoulins, F 94805 Villejuif, France
    Nat Med 13:54-61. 2007
    ..Blockade or knockdown of CRT suppressed the phagocytosis of anthracyclin-treated tumor cells by dendritic cells and abolished their immunogenicity in mice...
  72. ncbi Bacterial strategies for overcoming host innate and adaptive immune responses
    Mathias W Hornef
    Microbiology and Tumor Biology Center, Karolinska Institutet, Nobelsvag 16, SE 17177 Stockholm, Sweden
    Nat Immunol 3:1033-40. 2002
    ....
  73. ncbi Corpse clearance defines the meaning of cell death
    J Savill
    University of Edinburgh Medical Research Council Centre for Inflammation Research, Department of Clinical and Surgical Sciences, Internal Medicine, Royal Infirmary, UK
    Nature 407:784-8. 2000
    ..Far from being the end of the story, these clearance events allow scavenger cells to confer meaning upon cell death. But if the phagocytic 'spin doctors' receive or transmit the wrong messages, trouble ensues...
  74. pmc Novel cell death program leads to neutrophil extracellular traps
    Tobias A Fuchs
    Department for Cellular Microbiology, Max Planck Institute for Infection Biology, 10117 Berlin, Germany
    J Cell Biol 176:231-41. 2007
    ..This novel ROS-dependent death allows neutrophils to fulfill their antimicrobial function, even beyond their lifespan...
  75. ncbi Impaired macrophage phagocytosis in non-eosinophilic asthma
    J L Simpson
    Centre for Asthma and Respiratory Disease, The University of Newcastle, Newcastle, Australia
    Clin Exp Allergy 43:29-35. 2013
    ....
  76. pmc Cellular responses of Candida albicans to phagocytosis and the extracellular activities of neutrophils are critical to counteract carbohydrate starvation, oxidative and nitrosative stress
    Pedro Miramón
    Department of Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute, Jena, Germany
    PLoS ONE 7:e52850. 2012
    ..starvation response, as indicated by up-regulation of glyoxylate cycle genes, was only induced upon phagocytosis of the fungus. Similarly, the nitrosative stress response was only observed in internalised fungal cells...
  77. ncbi Role of phospholipids in endocytosis, phagocytosis, and macropinocytosis
    Michal Bohdanowicz
    Division of Cell Biology, Hospital for Sick Children, University of Toronto, Toronto, Canada
    Physiol Rev 93:69-106. 2013
    Endocytosis, phagocytosis, and macropinocytosis are fundamental processes that enable cells to sample their environment, eliminate pathogens and apoptotic bodies, and regulate the expression of surface components...
  78. pmc Cdc42, Rac1, and Rac2 display distinct patterns of activation during phagocytosis
    Adam D Hoppe
    Department of Microbiology and Immunology and the Biophysics Research Division, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
    Mol Biol Cell 15:3509-19. 2004
    ..Cdc42, Rac1, and Rac2 regulate the rearrangements of actin and membrane necessary for Fcgamma receptor-mediated phagocytosis by macrophages...
  79. ncbi Eaten alive! Cell death by primary phagocytosis: 'phagoptosis'
    Guy C Brown
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK
    Trends Biochem Sci 37:325-32. 2012
    Phagoptosis, also called primary phagocytosis, is a recently recognised form of cell death caused by phagocytosis of viable cells, resulting in their destruction...
  80. ncbi Potent phagocytic activity with impaired antigen presentation identifying lipopolysaccharide-tolerant human monocytes: demonstration in isolated monocytes from cystic fibrosis patients
    Carlos del Fresno
    Research Unit, Laboratory of Tumor Immunology, La Paz Hospital, Madrid, Spain
    J Immunol 182:6494-507. 2009
    ..Our results indicate that a tolerant phenotype interferes with timing, efficiency, and outcome of the innate immune responses against bacterial infections...
  81. ncbi Inhibition of microglial phagocytosis is sufficient to prevent inflammatory neuronal death
    Jonas J Neher
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom
    J Immunol 186:4973-83. 2011
    It is well-known that dead and dying neurons are quickly removed through phagocytosis by the brain's macrophages, the microglia...
  82. ncbi Cellular internalization of exosomes occurs through phagocytosis
    Du Feng
    School of Life Sciences, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, PR China
    Traffic 11:675-87. 2010
    ..the plasma membrane of non-phagocytic cells, while in phagocytic cells these exosomes were found to enter via phagocytosis. Specifically, they moved to phagosomes together with phagocytic polystyrene carboxylate-modified latex beads (..
  83. pmc Fine tuning inflammation at the front door: macrophage complement receptor 3-mediates phagocytosis and immune suppression for Francisella tularensis
    Shipan Dai
    Center for Microbial Interface Biology, Department of Microbial Infection and Immunity, The Ohio State University, Columbus, Ohio, United States of America
    PLoS Pathog 9:e1003114. 2013
    ..tularensis and CR3 is an important receptor for its phagocytosis. Here we confirm that efficient attachment and uptake of the highly virulent Type A F. tularensis spp...
  84. pmc CD47 is an adverse prognostic factor and therapeutic antibody target on human acute myeloid leukemia stem cells
    Ravindra Majeti
    Department of Internal Medicine, Division of Hematology, Stanford University, Palo Alto, CA 94304, USA
    Cell 138:286-99. 2009
    ..We hypothesized that increased CD47 expression on human AML LSC contributes to pathogenesis by inhibiting their phagocytosis through the interaction of CD47 with an inhibitory receptor on phagocytes...
  85. ncbi NETs: a new strategy for using old weapons
    Venizelos Papayannopoulos
    Department for Cellular Microbiology, Max Planck Institute for Infection Biology, Berlin, Germany
    Trends Immunol 30:513-21. 2009
    ..Furthermore, NETs may contribute to microbial containment by forming a physical barrier and a scaffold, to enhance antimicrobial synergy while minimizing damage to host tissues. Their role in innate immunity is only now being uncovered...
  86. ncbi PPARγ/RXRα-induced and CD36-mediated microglial amyloid-β phagocytosis results in cognitive improvement in amyloid precursor protein/presenilin 1 mice
    Mitsugu Yamanaka
    Clinical Neuroscience Unit, Department of Neurology, University of Bonn Medical Center, 53127 Bonn, Germany
    J Neurosci 32:17321-31. 2012
    ..gene expression, we tested whether activation of PPARγ would also result in improved microglial Aβ phagocytosis. The PPARγ agonist pioglitazone and a novel selective PPARα/γ modulator, DSP-8658, currently in clinical ..
  87. ncbi Divalent-metal transport by NRAMP proteins at the interface of host-pathogen interactions
    J R Forbes
    Dept of Biochemistry and Center for the Study of Host Resistance, McGill University, H3G 1Y6, Montreal, Canada
    Trends Microbiol 9:397-403. 2001
    ..Interestingly, bacterial and mammalian NRAMP proteins would compete for the same essential substrates within the microenvironment of the phagosome, at the interface of host-pathogen interactions...
  88. pmc Staphylococcus aureus biofilms prevent macrophage phagocytosis and attenuate inflammation in vivo
    Lance R Thurlow
    Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198, USA
    J Immunol 186:6585-96. 2011
    ..aureus biofilms in vitro revealed that macrophages successful at biofilm invasion displayed limited phagocytosis and gene expression patterns reminiscent of alternatively activated M2 macrophages...
  89. pmc MFG-E8 mediates primary phagocytosis of viable neurons during neuroinflammation
    Michael Fricker
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, UK
    J Neurosci 32:2657-66. 2012
    ..However, we show here that MFG-E8 can mediate phagocytosis of viable neurons during neuroinflammation induced by lipopolysaccharide (LPS), thereby causing neuronal death...
  90. pmc CX3CR1 in microglia regulates brain amyloid deposition through selective protofibrillar amyloid-β phagocytosis
    Zhiqiang Liu
    Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611, USA
    J Neurosci 30:17091-101. 2010
    ..revealed that these cells were highly effective at the uptake of protofibrillar amyloid but were incapable of phagocytosis of fibrillar congophilic Aβ...
  91. ncbi Phosphatidylinositol-phosphates mediate cytoskeletal reorganization during phagocytosis via a unique modular protein consisting of RhoGEF/DH and FYVE domains in the parasitic protozoon Entamoeba histolytica
    Kumiko Nakada-Tsukui
    Department of Parasitology, National Institute of Infectious Diseases, Shinjuku ku, Tokyo, Japan
    Cell Microbiol 11:1471-91. 2009
    To understand the roles of phosphoinositides [PtdIns] in phagocytosis of parasitic eukaryotes, we examined the interaction of phosphatidylinositol-3-phosphate [PtdIns(3)P] and putative PtdIns-P-binding proteins during phagocytosis in the ..
  92. pmc Toll-like receptor stimulation enhances phagocytosis and intracellular killing of nonencapsulated and encapsulated Streptococcus pneumoniae by murine microglia
    Sandra Ribes
    Department of Geriatrics, Evang Krankenhaus Göttingen Weende, An der Lutter 24, D 37075 Gottingen, Germany
    Infect Immun 78:865-71. 2010
    ..05). The presence of cytochalasin D, an inhibitor of actin polymerizaton, blocked >90% of phagocytosis. Along with an increased phagocytic activity, the intracellular bacterial killing was also increased in TLR-..
  93. pmc Glial precursors clear sensory neuron corpses during development via Jedi-1, an engulfment receptor
    Hsiao Huei Wu
    The Center for Molecular Neuroscience, Kennedy Center for Human Development, and Department of Biochemistry, Vanderbilt University Medical School, Nashville, Tennessee, USA
    Nat Neurosci 12:1534-41. 2009
    ..Together, these results suggest a cellular and molecular mechanism by which neuronal corpses are culled during DRG development...
  94. ncbi Non-redundant role of the long pentraxin PTX3 in anti-fungal innate immune response
    Cecilia Garlanda
    Department of Immunology and Cell Biology, Mario Negri Institute for Pharmacological Research, 20157 Milan, Italy
    Nature 420:182-6. 2002
    ..Thus, the long pentraxin Ptx3 is a secreted pattern-recognition receptor that has a non-redundant role in resistance to selected microbial agents, in particular to the opportunistic fungal pathogen Aspergillus fumigatus...
  95. ncbi Microglial phagocytosis induced by fibrillar beta-amyloid and IgGs are differentially regulated by proinflammatory cytokines
    Jessica Koenigsknecht-Talboo
    Department of Neurosciences, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA
    J Neurosci 25:8240-9. 2005
    ..We report that proinflammatory cytokines attenuate microglial phagocytosis stimulated by fAbeta or complement receptor 3 and argue that this may, in part, underlie the accumulation of ..
  96. pmc Age related changes in microglial phenotype vary between CNS regions: grey versus white matter differences
    Adam D Hart
    Centre for Biological Sciences, University of Southampton, Southampton General Hospital, Southampton, UK
    Brain Behav Immun 26:754-65. 2012
    ..Taken together, these findings show that the effects of age on microglial phenotype and functional integrity vary significantly between CNS compartments, as do, albeit to a lesser extent, the effects of systemic LPS...
  97. pmc Pseudomonas aeruginosa evasion of phagocytosis is mediated by loss of swimming motility and is independent of flagellum expression
    Eyal Amiel
    Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA
    Infect Immun 78:2937-45. 2010
    ..of motility, not the loss of the flagellum per se, is the critical factor in the development of resistance to phagocytosis by P. aeruginosa. We demonstrate that isogenic P...
  98. pmc Programmed cell clearance: molecular regulation of the elimination of apoptotic cell corpses and its role in the resolution of inflammation
    Bengt Fadeel
    Division of Molecular Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden
    Biochem Biophys Res Commun 396:7-10. 2010
    ..The present review will focus on the molecular regulation of programmed cell clearance, and discuss the role of cell elimination in the resolution of inflammation...
  99. ncbi Myeloperoxidase: friend and foe
    Seymour J Klebanoff
    Department of Medicine, University of Washington School of Medicine, Seattle, WA 98195 7185, USA
    J Leukoc Biol 77:598-625. 2005
    Neutrophilic polymorphonuclear leukocytes (neutrophils) are highly specialized for their primary function, the phagocytosis and destruction of microorganisms...
  100. pmc Response to Staphylococcus aureus requires CD36-mediated phagocytosis triggered by the COOH-terminal cytoplasmic domain
    Lynda M Stuart
    Laboratory of Developmental Immunology, Department of Pediatrics, Harvard Medical School, Boston, MA 02114, USA
    J Cell Biol 170:477-85. 2005
    ..aureus in vivo resulting in profound bacteraemia. Thus, response to S. aureus requires CD36-mediated phagocytosis triggered by the COOH-terminal cytoplasmic domain, which initiates TLR2/6 signaling.
  101. pmc Rapid neutrophil destruction following phagocytosis of Staphylococcus aureus
    Scott D Kobayashi
    Laboratory of Human Bacterial Pathogenesis, Research Technologies Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    J Innate Immun 2:560-75. 2010
    ..To better understand this phenomenon, we investigated the basis of rapid PMN lysis after phagocytosis of USA300, a prominent CA-MRSA strain...

Research Grants74

  1. Periodontal Biomaterials with BITE (Biofilm Immunity via T-cell Enhancement)
    James D Bryers; Fiscal Year: 2012
    ..designed to enhance the coupling of pathogenic oral bacteria to monocyte-macrophage (MX);thus promoting phagocytosis. For long-term protection, the biomaterial will transfect antigen-presenting cells (specifically dendritic ..
  2. Effect of PPARy Ligands on Alcohol-Induced Alveolar Macrophage Oxidative Stress
    Samantha M Yeligar; Fiscal Year: 2013
    ..oxygen species (ROS), and Nox2 is essential for the respiratory burst involved in killing microbes after phagocytosis. However, excessive ROS production suppresses phagocytosis...
  3. Statin modulation of immunotherapy for Alzheimer disease
    Jun Tan; Fiscal Year: 2012
    ..Two major hypotheses regarding the actions of A( immunotherapy are the facilitation of microglial phagocytosis via Fc receptors and promotion of A( removal from the brain by increasing the brain/blood concentration ..
  4. Group A Streptococcus responses to oxidative stress
    Ioannis Gryllos; Fiscal Year: 2010
    ..Moreover, construction and testing of a polA.1 mutant against oxidative stress challenge in vitro, or against phagocytosis by human leukocytes, will assess the contribution of PolA...
  5. Human neutrophils, phospholipase A2 and S.aureus: microbial targets and responses
    William M Nauseef; Fiscal Year: 2013
    ..Furthermore, we have identified transcriptional and structural responses by SA immediately following phagocytosis. We suspect that such changes contribute to the capacity of some ingested SA to survive in PMN and subsequently ..
  6. Mechanisms of Heme Transport in Macrophages
    CARINE CARINE WHITE; Fiscal Year: 2012
    ..the reticuloendothelial system, macrophages (M&s) play a major role in the recycling of heme-iron via phagocytosis of senescent red blood cells (i.e. erythrophagocytosis), and proteolytic degradation of hemoglobin...
  7. A Ribozyme Rescue Strategy for Dry Age-Related Macular Degeneration
    John M Sullivan; Fiscal Year: 2013
    ..in part, naturally occurring circadian shedding of rod and cone photoreceptor (PR) outer segment tips, and phagocytosis and lysosomal digestion by RPE cells...
  8. Pulmonary effects of biomass fuel indoor PM from rural India
    Shyam Biswal; Fiscal Year: 2013
    ..We will also determine whether exposure of macrophages to indoor PM suppresses phagocytosis of bacteria...
  9. Anthrax Toxins Impair Phagocyte Actin-based Motility
    Frederick Southwick; Fiscal Year: 2010
    ..B. Examine the effects of PA + EF, and PA + LF + EF on neutrophil chemotaxis, phagocytosis, and actin assembly. C...
  10. Group B. Streptococci and Toll-like Receptors
    Douglas T Golenbock; Fiscal Year: 2012
    ..Next, we plan to define the roles of GBS phagocytosis (or invasion) and modification of GBS inside phagocytes for the inflammatory phenotype...
  11. COCHLEAR MACROPHAGES AND EPITHELIAL REPAIR
    Keiko Hirose; Fiscal Year: 2013
    ..Additional studies will examine the involvement of the cardinal scavenger receptor, CD36, in the phagocytosis of injured hair cells...
  12. TOLLIP and Tuberculosis Immunopathogenesis
    JAVEED ALI SHAH; Fiscal Year: 2013
    ..experience in macrophage biology and will allow the applicant to study the role of TOLLIP variation on phagocytosis and autophagy...
  13. Dysregulation of the inflammatory response by Francisella tularensis
    Lee Ann H Allen; Fiscal Year: 2013
    ..cells that are preprogrammed to die by constitutive apoptosis, a process that is typically accelerated by phagocytosis and is essential for resolution of the inflammatory response. In marked contrast, we find that F...
  14. White Matter Protection in Cerebral Ischemia
    Guodong Cao; Fiscal Year: 2013
    ..We found that NAMPT also enhances microglial myelin phagocytosis. This is important because damaged myelin is believed to suppress the maturation of OPCs and cause ..
  15. Disease-related defects in dendritic cell processing of bacterial antigens
    Michael S Marks; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Bacterial infections are largely cleared by phagocytosis within specialized phagocytic cell types...
  16. Screen for Small Molecule Regulators of Retinal Progenitor Epithelial Cell Phagoc
    CHARLES YOON HYUNG CHO; Fiscal Year: 2010
    ..in C-mer proto-oncogene tyrosine kinase, also known as MERTK, have been associated with disruption of the RPE phagocytosis pathway and autosomal recessive RP in humans. The molecular mechanisms of RPE phagocytosis are still unclear...
  17. Otitis media-associated pneumococcal genes
    Honggao Yan; Fiscal Year: 2013
    ..pneumoniae resistance to host phagocytosis, a major host effector mechanism against Gram-positive pathogens...
  18. Bordetella Cyclase Toxin:Effect on Leukocytes and Respiratory Epithelial Cells
    Erik L Hewlett; Fiscal Year: 2013
    ..of ACT on: (1A) the viability of human PMN, distinguishing among the known pathways leading to PMN death;(1B) phagocytosis, generation of reactive-oxygen species, NET formation and bacterial killing by PMN;and (1C) the production of ..
  19. Harness Germinal Matrix Hemorrhage
    John H Zhang; Fiscal Year: 2013
    ..of peroxisome proliferator-activated receptor gamma (PPAR-?), which has been reported to increase microglial phagocytosis of red blood cells, thus decreasing residual clot sizes...
  20. Mac-1 (CR3) and Fc gamma receptors in immune-mediated neutrophil cytotoxicity
    Tanya N Mayadas; Fiscal Year: 2013
    ..The objective of Aim I is to delineate mechanisms of neutrophil phagocytosis that are relevant for complement C3 mediated tissue injury and host defense...
  21. Bacterial Stress Responses to Intestinal Inflammation
    JONATHAN JAMES HANSEN; Fiscal Year: 2013
    ..uptake by macrophages and promotes intracellular survival using genetically altered NC101 and inhibitors of phagocytosis and ROS generation in gentamicin protection assays. 3) Examine the biological relevance of E...
  22. Analysis of NKLAM: A Novel Gene Associated With Cellular Cytotoxicity
    Jacki Kornbluth; Fiscal Year: 2013
    ..The role of NKLAM in TLR-mediated signaling events and in macrophage phagocytosis will be investigated. 3) Characterize NKLAM KO mice to study the role of NKLAM in vivo...
  23. Age-dependent microglial responses in hypoxia-ischemia
    PETER A FERRAZZANO; Fiscal Year: 2013
    ..In the immature brain, microglial cells are activated for phagocytosis of cellular debris during synaptogenesis, and it is unknown how this predominance of activated microglia ..
  24. Role of Natural Killer Lytic-Associated Molecule (NKLAM) in Natural Killer Functi
    Jacki Kornbluth; Fiscal Year: 2010
    ..proposal describes studies to: 1) Determine the role of NKLAM in cell-mediated cytotoxicity and in macrophage phagocytosis. Experiments are described to determine whether, upon degranulation, NKLAM enters target cells and enhances ..
  25. NADPH-Oxidase and SLE Susceptibility
    Swapan K Nath; Fiscal Year: 2013
    ..invading microbial pathogens, and are produced by the multi-protein NADPH- oxidase (NADPHO) system during phagocytosis. This multi-protein complex is encoded by 7 essential genes: NCF1, NCF2, NCF4, CYBA,CYBB, Rac1 or Rac2...
  26. Novel anti-toxin compounds against Yersinia infections
    Maurizio Pellecchia; Fiscal Year: 2010
    ..PTPase that is results in disruption of focal adhesions, and inhibition of integrin-mediated bacterial phagocytosis, both of which are highly dependent on tyrosine phosphorylation...
  27. Enhancing Nrf2 by Sulforaphane Treatment in COPD
    Robert A Wise; Fiscal Year: 2012
    ..decreases oxidative stress, increases proteasomal anti-apoptotic cytoprotective responses, improves bacterial phagocytosis and killing, and reverses tobacco-smoke induced corticosteroid resistance...
  28. Phagosomal Signals Shape Inflammatory Responses to Borrelia Burgdorferi
    Juan C Salazar; Fiscal Year: 2010
    ..More recently, we have provided extensive evidence that phagocytosis of live spirochetes, by either human monocytes or murine macrophages, generates a more intense and far broader ..
  29. Interactions between P. aeruginosa and autophagy in Airway epithelial cells
    INDHIRA H DE LA ROSA; Fiscal Year: 2013
    ..aeruginosa T3SS can negatively alter other antimicrobial pathways, including phagocytosis (3). Our hypothesis is that P. aeruginosa T3SS inhibits autophagy thereby protecting P...
  30. Pleiotropic Transcription Factors As Target For Intracerebral Hemorrhage Treatmen
    Jaroslaw A Aronowski; Fiscal Year: 2012
    ..Nrf2), a pleiotropic protein that regulates transcription of a battery of key cytoprotective, detoxifying, and phagocytosis-regulating genes represents a promising target for ICH therapy...
  31. FUNCTIONAL HIERARCHY OF REMNANT LIPOPROTEIN RECEPTORS
    Sergio Fazio; Fiscal Year: 2013
    ..macrophage lives in a peculiar environment, where cholesterol excesses trigger diverging signals of active phagocytosis and inflammatory bursts...
  32. Homing Receptor Determinants of HSC
    Irving L Weissman; Fiscal Year: 2013
    ..of HSC homing to and from BM sites under steady state conditions;2) To establish whether blockade of macrophage phagocytosis by CD47 enables mouse and human HSC homing to hematopoietic microenvironments and 3) To isolate and ..
  33. The Transport of Nutritional Heme in Animal Development
    Iqbal Hamza; Fiscal Year: 2013
    ..In humans, greater than 60% of total body iron is present as heme in hemoglobin. Iron from heme is recycled by phagocytosis of senescent red blood cells and proteolytic digestion of hemoglobin in macrophages...
  34. Effects of apoptotic epithelial cell engulfment on mucosal inflammation
    Peter B Ernst; Fiscal Year: 2010
    ..APC), including macrophages and dendritic cells, can remove bacteria, cellular debris and dead cells through phagocytosis or autophagy...
  35. Role of complement in alpha-synuclein based models of Parkinson disease
    HEATHER ELIZABETH ALLEN; Fiscal Year: 2013
    ..components expressed by microglia, changes in surface complement receptors by flow cytometry, changes in phagocytosis by commercially available assays, and changes in cytokine expression by ELISA...
  36. Role of Protein Kinase C in Macrophage Activation
    Michelle R Lennartz; Fiscal Year: 2012
    Macrophage phagocytosis is the first line of defense against infection. Normally, Fc?R-mediated phagocytosis efficiently clears pathogens and presents antigen to the adaptive immune system...
  37. Mannose Binding Lectin in Neuroinflammation and NeuroAIDS
    Kumud K Singh; Fiscal Year: 2013
    ..role of mannose binding lectin, an important component of complement-mediated innate immunity and pathogen phagocytosis, in susceptibility of HIV-1 infection in brain and related neuroinflammation, viral protein accumulation and ..
  38. Investigating gene x environment interaction using human exposures to O3 & LPS
    David B Peden; Fiscal Year: 2010
    ..monocytes, macrophages and dendritic cells, airway and circulating phagocyte function (oxidative burst and phagocytosis of opsonized zymosan) and mediator secretion...
  39. Apoptotic T Cell Clearance From Murine Lungs
    Jeffrey L Curtis; Fiscal Year: 2010
    ..Techniques will include assays of phagocytosis and adhesion;immunoprecipitation and Western analysis;real-time PCR;confocal microscopy;transient transfections;..
  40. A Novel Therapy for Septic Shock
    Xiaoling Qiang; Fiscal Year: 2011
    ..Early administration of rat MFG-E8-containing exosomes or recombinant murine MFG-E8 (rmMFG- E8) increases phagocytosis of apoptotic cells, reduces proinflammatory cytokines, and improves survival in a rodent model of septic shock...
  41. Apoptosis and efferocytosis: regulators of immunity to tuberculosis
    Samuel M Behar; Fiscal Year: 2013
    ..Here we put forward a new hypothesis that it is not apoptosis per se that leads to control, but instead phagocytosis of Mtb infected apoptotic M[unreadable] that is the crucial event...
  42. Molecular Mechanism of Entamoeba histolytica phagocytosis
    Christopher D Huston; Fiscal Year: 2012
    ..Entamoeba histolytica, the etiologic agent, is an intestinal ameba that acquires nutrients by phagocytosis of colonic bacteria, and phagocytosis of host cells by E...
  43. Employing Familial AD Induced Pluripotent Stem Cells to Study Neurodegeneration
    Suman Jayadev; Fiscal Year: 2013
    ..C) Differentiate the iPSC lines used in 1B into microglia and evaluate for pro-inflammatory cytokine release, phagocytosis and inflammatory pathway signaling...
  44. Role of mucin-type O-glycosylation in Trypanosoma cruzi biology
    Christopher M West; Fiscal Year: 2012
    ..GIPLs) and mucin-type glycoproteins protect the parasites from proteases, complement, lytic antibodies, phagocytosis, and function as potential immunomodulators that could even determine the fate of pathogenesis during infection...
  45. Oxidative Lipidomics in Pediatric Traumatic Brain Injury
    Hulya Bayir; Fiscal Year: 2012
    ..of apoptotic factors from mitochondria into cytosol, and externalization of PS marking apoptotic cells for phagocytosis. Our hypothesis is that TBI initiates excessive production of ROS and selective early oxidation of ..
  46. Human Macrophage Sodium Channels: Novel Targets for Inflammatory Diseases
    Michael D Carrithers; Fiscal Year: 2013
    ..pathogen biosensor to enhance inflammatory responses and that it regulates macrophage endosomal acidification, phagocytosis, and inflammatory signaling...
  47. Targeting copper homeostasis in the fungal pathogen, Cryptococcus neoformans
    RICHARD FESTA; Fiscal Year: 2013
    ..to evaluate mutants in Cuf1-dependent genes to ascertain their contribution to survival to alveolar macrophage phagocytosis in vitro...
  48. Genetic Basis of Virulence of Community MRSA Clone USA300
    Henry F Chambers; Fiscal Year: 2010
    ..PMN function will be assayed in vitro to determine whether virulence factors affect human PMN chemotaxis, phagocytosis, or bacterial killing...
  49. Innate immune suppression following experimental injury
    KRISTIN C GREATHOUSE; Fiscal Year: 2012
    ..we plan to measure the ability of leukocytes in whole blood to perform the critical antimicrobial functions of phagocytosis and killing in a mouse model of injury-induced immune suppression (partial hepatectomy), and hypothesize that ..
  50. Hypercapnia and neutrophil function in pulmonary host defense
    Khalilah L Gates; Fiscal Year: 2013
    ..by inhibition of several NF-?B-regulated pro-inflammatory cytokines critical for host defense and impairs phagocytosis. Furthermore, prolonged hypercapnia in the murine Pseudomonas aeruginosa pneumonia model suggests that ..
  51. Identification of G6PD inhibitors for the development of novel antimalarial drugs
    Lars Bode; Fiscal Year: 2010
    ..occurring G6PD deficiency leads to a lack of reducing equivalents, an increase in oxidative stress, enhanced phagocytosis of parasite-infected RBCs, and, as a consequence, to a protection against malaria...
  52. THE STRESS-ACTIVATED PROTEIN KINASE PATHWAY
    John M Kyriakis; Fiscal Year: 2010
    ..the role of GCK and its effectors in PAMP-stimulated cellular inflammatory responses (cytokine induction, phagocytosis and monocyte-endothelial cell interaction)...
  53. HMGB1 and neutrophil efferocytosis.
    JAROSLAW WALDEMAR ZMIJEWSKI; Fiscal Year: 2012
    ..In recent studies, we found that HMGB1 inhibits phagocytosis of apoptotic neutrophils by macrophages in vivo and in vitro...
  54. Role of Epithelial Chemokines in Kidney Injury-Molecule-1 Induced Renal Fibrosis
    Benjamin D Humphreys; Fiscal Year: 2010
    ..2) Does expression of Kim-1 and/or activation of Kim-1 by phagocytosis trigger acute activation of the NF-kB pathway, resulting in expression of pro-inflammatory signals? We will use ..
  55. Endogenous Hemoglobin And Heme Detoxification After Intracerebral Hemorrhage
    Xiurong Zhao; Fiscal Year: 2013
    ..Clearance of cell-free Hb/heme through phagocytosis/endocytosis is an essential function of microglia/macrophages in anti-inflammatory response...
  56. Molecular Basis of the Anti-inflammatory Action of MAYP/PSTPIP2
    Violeta Chitu; Fiscal Year: 2012
    ..2. Establish the mechanism by which MAYP deficiency impairs phagocytosis. 3. Determine and utilize the three dimensional structure of MAYP to identify functionally important regions...
  57. Pneumococcal capsule and host innate immunity
    Moon H Nahm; Fiscal Year: 2012
    ..11A but not against 11E isolates by testing L-ficolin's ability to opsonize serotype 11A but not 11E for phagocytosis, by measuring the serum levels of L-ficolin and MASP-2 in individuals with serotype 11A pneumococcal sepsis, ..
  58. MECHANISMS OF ARP2/3 ACTIVATION AND FUNCTION
    R Dyche Mullins; Fiscal Year: 2013
    ..addition to powering locomotion, such networks also underlie other fundamental cellular processes, including: phagocytosis, endocytosis, movement of intracellular pathogens, and healing of membrane ruptures...
  59. KIDNEY INJURY MOLECULE-1 IN EPITHELIAL REPAIR
    JOSEPH VINCENT BONVENTRE; Fiscal Year: 2013
    ..A mutant mouse lacking an extracellular domain that is important for phagocytosis and a transgenic mouse with Kim-1 expression in the renal tubule have important phenotypes supporting a ..
  60. Non-oxidative Resistance to A. fumigatus by Alveolar Macrophages
    James B Burritt; Fiscal Year: 2010
    ..and gp91phox-/- mice is not significantly different when triggered by oxidant-triggering stimulants, or by phagocytosis of fungal particles...
  61. GM-CSF for Immunomodulation Following Trauma (GIFT) Trial
    Mark W Hall; Fiscal Year: 2013
    ..five measures of innate immune function (cytokine production capacity, antigen presenting capacity, migration, phagocytosis, and microbial killing) with infection risk, with GM-CSF responsiveness, and with each other...
  62. The Impact of Zinc on Innate Immunity and Patient Safety in Sepsis
    BETH YVONNE BESECKER; Fiscal Year: 2013
    ..Recently she has been involved in translational research evaluating the role of zinc in bacterial phagocytosis and clearance in sepsis. Dr...
  63. Mycobacterium avium subspecies in Crohn's Disease
    Saleh Naser; Fiscal Year: 2005
    ..of CD patients have been reported, both in the ability of the cell to phagocytose and in immune killing after phagocytosis, The cytokine pattern in CD is Th1-like and defect in the ratio of proinflammatory to anti- inflammatory ..
  64. Fc receptor targeted therapy for immune hemolytic anemia
    Periasamy Selvaraj; Fiscal Year: 2009
    ..Many studies have shown that the Fc[unreadable]Rs expressed on phagocytes play a vital role in binding and phagocytosis of antibody-coated RBCs...
  65. A forward genetics screen for genes regulating phagocytosis and signaling in Enta
    Lesly A Temesvari; Fiscal Year: 2010
    ..Two cellular processes that may play a role in pathogenicity are phagocytosis and PI 3-kinase-based signal transduction...
  66. Determination of a Mechanism for the Thrombocytopenia of WAS
    Ted S Strom; Fiscal Year: 2010
    ..results in a murine model of WAS demonstrate both rapid in vivo platelet consumption and enhanced ex-vivo phagocytosis of opsonized WASP(-) platelets by macrophages...
  67. Mechanisms of Microglial Activation and Phagocytosis in*
    Gary Landreth; Fiscal Year: 2005
    ..with the amyloid fibrils and mediates the pro-inflammatory activation of microglia and stimulation of phagocytosis in vitro...
  68. CR3 IN CELL ACTIVATION AND PHAGOCYTOSIS OF GONORRHEA
    ROBIN INGALLS; Fiscal Year: 2001
    ..The focus of this proposal is to identify the molecular mechanisms involved in phagocytosis and LPS signal transduction using CR3- and CR4-transfected fibroblast cell lines...
  69. E. histolytica phagocytosis of apoptotic host cells
    Christopher Huston; Fiscal Year: 2007
    ..Apoptotic host cell killing and phagocytosis further characterize E. histolytica infection. Indeed, clinicians use erythrophagocytosis to distinguish E...
  70. MECHANISMS OF PNEUMOCYSTIS CARINII PNEUMONIA
    William Martin; Fiscal Year: 1999
    ..Pc attachment, however, may not necessarily trigger effective phagocytosis or killing of the organism...
  71. Role of CIC-3 channels in regulation of cell volume and shape in neutrophils
    Jessica Moreland; Fiscal Year: 2007
    ..Chemotaxis, phagocytosis, and bactericidal activity are functions integral to the contribution of polymorphonuclear leukocytes (PMN) to ..
  72. Mechanism of Host Cell Invasion by Microbial Pathogens
    Partho Ghosh; Fiscal Year: 2010
    ..bacterial pathogens gain intracellular entry into host cell types that are normally non-phagocytic by inducing phagocytosis. Intracellular invasion is crucial to the virulence of these pathogens, enabling them to evade certain immune ..
  73. Mechanism of anti-phagocytosis by Yersinia pestis
    Kristiina Vuori; Fiscal Year: 2005
    ..bacteria are effectively ingested by integrin-mediated engulfment and destroyed by phagocytes, Yersinia blocks phagocytosis. The mechanism of "anti-phagocytosis" involves the virulence factor YopH, a highly), effective tyrosine ..
  74. A Role for Melanoregulin in RPE-mediated Phagocytosis
    KATHLEEN B BOESZE BATTAGLIA; Fiscal Year: 2010
    ..only a high degradative load but since these cells are post-mitotic, over the long-term, virtually continuous phagocytosis produces additional metabolic stress on the RPE...