g1 phase

Summary

Summary: The period of the CELL CYCLE preceding DNA REPLICATION in S PHASE. Subphases of G1 include "competence" (to respond to growth factors), G1a (entry into G1), G1b (progression), and G1c (assembly). Progression through the G1 subphases is effected by limiting growth factors, nutrients, or inhibitors.

Top Publications

  1. ncbi Persistent nicotine treatment potentiates amplification of the dihydrofolate reductase gene in rat lung epithelial cells as a consequence of Ras activation
    Jinjin Guo
    Cancer Research Center, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 280:30422-31. 2005
  2. ncbi Cancer cell cycles
    C J Sherr
    Howard Hughes Medical Institute, Department of Tumor Cell Biology, St Jude Children s Research Hospital, 332 North Lauderdale, Memphis, TN 38105, USA
    Science 274:1672-7. 1996
  3. ncbi Lengthened G1 phase indicates differentiation status in human embryonic stem cells
    Ashley Calder
    McMaster Stem Cell and Cancer Research Institute, Michael G DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada
    Stem Cells Dev 22:279-95. 2013
  4. ncbi G1 cell-cycle control and cancer
    Joan Massague
    Cancer Biology and Genetics Program, and Howard Hughes Medical Institute, Box 116, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York 10021, USA
    Nature 432:298-306. 2004
  5. ncbi The effects of molecular noise and size control on variability in the budding yeast cell cycle
    Stefano Di Talia
    The Rockefeller University, New York, New York 10021, USA
    Nature 448:947-51. 2007
  6. ncbi p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21
    H Toyoshima
    Molecular Biology and Virology Laboratory, Salk Institute, La Jolla, California 92037
    Cell 78:67-74. 1994
  7. ncbi To cycle or not to cycle: a critical decision in cancer
    M Malumbres
    Molecular Oncology Program, Centro Nacional de Investigaciones Oncologicas, Madrid, Spain
    Nat Rev Cancer 1:222-31. 2001
  8. ncbi Cln3 activates G1-specific transcription via phosphorylation of the SBF bound repressor Whi5
    Robertus A M de Bruin
    Department of Molecular Biology, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Cell 117:887-98. 2004
  9. ncbi Cyclin D-dependent kinases, INK4 inhibitors and cancer
    Sagrario Ortega
    Molecular Oncology Program, Centro Nacional de Investigaciones Oncologicas, Melchor Fernandez Almagro 3, 28029 Madrid, Spain
    Biochim Biophys Acta 1602:73-87. 2002
  10. ncbi Specific inhibition of cyclin-dependent kinase 4/6 by PD 0332991 and associated antitumor activity in human tumor xenografts
    David W Fry
    Cancer Pharmacology, Pfizer Global Research and Development, Ann Arbor, Michigan 48105, USA
    Mol Cancer Ther 3:1427-38. 2004

Detail Information

Publications376 found, 100 shown here

  1. ncbi Persistent nicotine treatment potentiates amplification of the dihydrofolate reductase gene in rat lung epithelial cells as a consequence of Ras activation
    Jinjin Guo
    Cancer Research Center, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 280:30422-31. 2005
    ..Under p53-deficient conditions, the establishment of a full oncogenic transformation, in response to long term nicotine exposure, is achieved through the cooperation of multiple signaling pathways...
  2. ncbi Cancer cell cycles
    C J Sherr
    Howard Hughes Medical Institute, Department of Tumor Cell Biology, St Jude Children s Research Hospital, 332 North Lauderdale, Memphis, TN 38105, USA
    Science 274:1672-7. 1996
    ..and cyclin D1, proteins that govern phosphorylation of the retinoblastoma protein (RB) and control exit from the G1 phase of the cell cycle, are so frequent in human cancers that inactivation of this pathway may well be necessary for ..
  3. ncbi Lengthened G1 phase indicates differentiation status in human embryonic stem cells
    Ashley Calder
    McMaster Stem Cell and Cancer Research Institute, Michael G DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada
    Stem Cells Dev 22:279-95. 2013
    The cell cycle in pluripotent stem cells is notable for the brevity of the G1 phase, permitting rapid proliferation and reducing the duration of differentiation signal sensitivity associated with the G1 phase...
  4. ncbi G1 cell-cycle control and cancer
    Joan Massague
    Cancer Biology and Genetics Program, and Howard Hughes Medical Institute, Box 116, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York 10021, USA
    Nature 432:298-306. 2004
    ....
  5. ncbi The effects of molecular noise and size control on variability in the budding yeast cell cycle
    Stefano Di Talia
    The Rockefeller University, New York, New York 10021, USA
    Nature 448:947-51. 2007
    ..This analysis decomposes the regulatory dynamics of the Start transition into two independent modules, a size sensing module and a timing module, each of which is predominantly controlled by a different G1 cyclin...
  6. ncbi p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21
    H Toyoshima
    Molecular Biology and Virology Laboratory, Salk Institute, La Jolla, California 92037
    Cell 78:67-74. 1994
    ..p27 is identical to p27Kip1, a cyclin-Cdk inhibitor present in TGF beta-treated cells. p27 has the hallmarks of a negative regulator of G1 progression and may mediate TGF beta-induced G1 arrest...
  7. ncbi To cycle or not to cycle: a critical decision in cancer
    M Malumbres
    Molecular Oncology Program, Centro Nacional de Investigaciones Oncologicas, Madrid, Spain
    Nat Rev Cancer 1:222-31. 2001
    ..Understanding the molecular mechanisms that underlie this commitment should provide important insights into how normal cells become tumorigenic and how new anticancer strategies can be devised...
  8. ncbi Cln3 activates G1-specific transcription via phosphorylation of the SBF bound repressor Whi5
    Robertus A M de Bruin
    Department of Molecular Biology, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Cell 117:887-98. 2004
    ..Whi5 associates with G1-specific promoters via SBF during early G1 phase, then dissociates coincident with transcriptional activation. Dissociation of Whi5 is promoted by Cln3 in vivo...
  9. ncbi Cyclin D-dependent kinases, INK4 inhibitors and cancer
    Sagrario Ortega
    Molecular Oncology Program, Centro Nacional de Investigaciones Oncologicas, Melchor Fernandez Almagro 3, 28029 Madrid, Spain
    Biochim Biophys Acta 1602:73-87. 2002
    ..As a consequence, some of these molecules are currently being considered as targets for cancer therapy, and several novel molecules, such as Cdk inhibitors, are under development as potential anti-cancer drugs...
  10. ncbi Specific inhibition of cyclin-dependent kinase 4/6 by PD 0332991 and associated antitumor activity in human tumor xenografts
    David W Fry
    Cancer Pharmacology, Pfizer Global Research and Development, Ann Arbor, Michigan 48105, USA
    Mol Cancer Ther 3:1427-38. 2004
    ..The results indicate that inhibition of Cdk4/6 alone is sufficient to cause tumor regression and a net reduction in tumor burden in some tumors...
  11. ncbi Cyclin E-CDK2 is a regulator of p27Kip1
    R J Sheaff
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center FHCRC, Seattle, Washington 98104, USA
    Genes Dev 11:1464-78. 1997
    ....
  12. ncbi Inhibition of eukaryotic DNA replication by geminin binding to Cdt1
    J A Wohlschlegel
    Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA
    Science 290:2309-12. 2000
    ..In the normal cell cycle, Cdt1 is present only in G1 and S, whereas geminin is present in S and G2 phases of the cell cycle. Together, these results suggest that geminin inhibits inappropriate origin firing by targeting Cdt1...
  13. ncbi Cyclins and cdks in development and cancer: a perspective
    Amit Deshpande
    Department of Radiation Oncology, Molecular Oncology Research Institute, Tufts New England Medical Center, Boston, MA 02111, USA
    Oncogene 24:2909-15. 2005
    ....
  14. ncbi CDK activity antagonizes Whi5, an inhibitor of G1/S transcription in yeast
    Michael Costanzo
    Department of Medical Genetics and Microbiology, University of Toronto, 1 King s College Circle, Toronto, M5S 1A8, Canada
    Cell 117:899-913. 2004
    ..CDK) activity initiates the eukaryotic cell division cycle by turning on a suite of gene expression in late G1 phase. In metazoans, CDK-dependent phosphorylation of the retinoblastoma tumor suppressor protein (Rb) alleviates ..
  15. pmc Reprogramming the pluripotent cell cycle: restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells
    Prachi N Ghule
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Cell Physiol 226:1149-56. 2011
    ..hES cells are characterized by an abbreviated G1 phase (∼ 2...
  16. ncbi Human embryonic stem cells exhibit increased propensity to differentiate during the G1 phase prior to phosphorylation of retinoblastoma protein
    Yogev Sela
    Sohnis and Forman Families Center for Stem Cell and Tissue Regeneration Research, Ruth and Bruce Rappaport Faculty of Medicine, Technion, Haifa, Israel
    Stem Cells 30:1097-108. 2012
    ..stem cells (hESCs) in G1 has been shown to stimulate differentiation, it remains unclear whether the unperturbed G1 phase in hESCs is causally related to differentiation...
  17. ncbi Downregulation of CCND1 and CDK6 by miR-34a induces cell cycle arrest
    Fang Sun
    Beijing Institute of Radiation Medicine, Beijing, People s Republic of China
    FEBS Lett 582:1564-8. 2008
    ..Taken together, our data suggest that the effects of miR-34a on G1 cell cycle arrest are through the down-regulation of CCND1 and CDK6, which is associated with other targets of miR-34a either additively or synergistically...
  18. pmc Functional inactivation of the retinoblastoma protein requires sequential modification by at least two distinct cyclin-cdk complexes
    A S Lundberg
    The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Mol Cell Biol 18:753-61. 1998
    ..Complete phosphorylation of pRb, inactivation of E2F binding, and activation of E2F transcription occur only after sequential action of at least two distinct G1 cyclin kinase complexes...
  19. ncbi The Pezcoller lecture: cancer cell cycles revisited
    C J Sherr
    Howard Hughes Medical Institute and Department of Tumor Cell Biology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Cancer Res 60:3689-95. 2000
    Genetic lesions that disable key regulators of G1 phase progression in mammalian cells are present in most human cancers...
  20. pmc Pathways of DNA double-strand break repair during the mammalian cell cycle
    Kai Rothkamm
    Fachrichtung Biophysik, Universitat des Saarlandes, D 66421 Homburg Saar, Germany
    Mol Cell Biol 23:5706-15. 2003
    ..These data provide the first evaluation of the cell cycle-specific contributions of NHEJ and HR to the repair of radiation-induced versus replication-associated DSBs...
  21. ncbi New functional activities for the p21 family of CDK inhibitors
    J LaBaer
    Massachusetts General Hospital Cancer Center, Charlestown 02129, USA
    Genes Dev 11:847-62. 1997
    ..We suggest that in addition to their roles as inhibitors, the p21 family of proteins, originally identified as inhibitors, may also have roles as adaptor proteins that assemble and program kinase complexes for specific functions...
  22. pmc TOR controls translation initiation and early G1 progression in yeast
    N C Barbet
    Department of Biochemistry, University of Basel, Switzerland
    Mol Biol Cell 7:25-42. 1996
    ..the immunosuppressant rapamycin or depleted for the targets of rapamycin TOR1 and TOR2 arrest growth in the early G1 phase of the cell cycle...
  23. ncbi The novel yeast PAS kinase Rim 15 orchestrates G0-associated antioxidant defense mechanisms
    Elisabetta Cameroni
    Department of Microbiology and Molecular Medicine, Centre Medical Universitaire, Univeristy of Geneva, Switzerland
    Cell Cycle 3:462-8. 2004
    ....
  24. ncbi How proteolysis drives the cell cycle
    R W King
    Department of Cell Biology, Harvard Medical School, 240 Longwood Ave, Boston, MA 02115, USA
    Science 274:1652-9. 1996
    ..Proteolysis therefore drives cell cycle progression not only by regulating CDK activity, but by directly influencing chromosome and spindle dynamics...
  25. ncbi Distinct roles for JNK1 and JNK2 in regulating JNK activity and c-Jun-dependent cell proliferation
    Kanaga Sabapathy
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, A 1030 Vienna, Austria
    Mol Cell 15:713-25. 2004
    ..In contrast, JNK1 becomes the major c-Jun interacting kinase after cell stimulation. These data provide mechanistic insights into the distinct roles of different JNK isoforms...
  26. pmc ATM and Artemis promote homologous recombination of radiation-induced DNA double-strand breaks in G2
    Andrea Beucher
    Darmstadt University of Technology, Radiation Biology and DNA Repair, Darmstadt, Germany
    EMBO J 28:3413-27. 2009
    ..We suggest that Artemis endonuclease removes lesions or secondary structures, which inhibit end resection and preclude the completion of HR or NHEJ...
  27. ncbi Cycling to cancer with cyclin D1
    J Alan Diehl
    The Leonard and Madlyn Abramson Family Cancer Research Institute, University of Pennsylvania Cancer Center, Department of Cancer Biology, Philadelphia, Pennsylvania 19104 USA
    Cancer Biol Ther 1:226-31. 2002
    ....
  28. ncbi High expression of active CDK6 in the cytoplasm of CD8 memory cells favors rapid division
    Henrique Veiga-Fernandes
    Institut National de la Santé et de la Recherche Médicale U591, Institut Necker, 156 rue de Vaugirard, 75015 Paris, France
    Nat Immunol 5:31-7. 2004
    ..Therefore G0/G1 memory cells are at a unique preactivated state that favors rapid division after antigen stimulation...
  29. pmc Phosphorylation-dependent ubiquitination of cyclin D1 by the SCF(FBX4-alphaB crystallin) complex
    Douglas I Lin
    The Leonard and Madlyn Abramson Family Cancer Research Institute and Cancer Center, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Mol Cell 24:355-66. 2006
    ..We conclude that SCF(FBX4-alphaB crystallin) is an E3 ubiquitin ligase that promotes ubiquitin-dependent degradation of Thr286-phosphorylated cyclin D1...
  30. ncbi Activation of p53 by MDM2 antagonists can protect proliferating cells from mitotic inhibitors
    Daisy Carvajal
    Discovery Oncology, Roche Research Center, Hoffmann La Roche Inc, Nutley, New Jersey 07110, USA
    Cancer Res 65:1918-24. 2005
    ..Second, pretreatment with MDM2 antagonists before chemotherapy of tumors with mutant p53 may offer a partial protection to proliferating normal tissues...
  31. ncbi Translational control of p27Kip1 accumulation during the cell cycle
    L Hengst
    Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Science 271:1861-4. 1996
    ....
  32. ncbi DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts
    A Di Leonardo
    Gene Expression Lab, Salk Institute, La Jolla, California 92037
    Genes Dev 8:2540-51. 1994
    ..We propose that p53 helps maintain genetic stability in NDF by mediating a permanent cell cycle arrest through long-term induction of Cip1 when low amounts of unrepaired DNA damage are present in G1 before the restriction point...
  33. pmc A mechanism for cell-cycle regulation of MAP kinase signaling in a yeast differentiation pathway
    Shelly C Strickfaden
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 128:519-31. 2007
    Yeast cells arrest in the G1 phase of the cell cycle upon exposure to mating pheromones. As cells commit to a new cycle, G1 CDK activity (Cln/CDK) inhibits signaling through the mating MAPK cascade...
  34. pmc Interactions among LRF-1, JunB, c-Jun, and c-Fos define a regulatory program in the G1 phase of liver regeneration
    J C Hsu
    Department of Human Genetics, University of Pennsylvania School of Medicine, Philadelphia 19104 6145
    Mol Cell Biol 12:4654-65. 1992
    ..Thus, through complex interactions among LRF-1, JunB, c-Jun, and c-Fos, control of delayed gene expression may be established for extended times during the G1 phase of hepatic growth.
  35. ncbi Hog1 mediates cell-cycle arrest in G1 phase by the dual targeting of Sic1
    Xavier Escote
    Cell Signaling Unit, Departament de Ciencies Experimentals i de la Salut, Universitat Pompeu Fabra, E 08003 Barcelona, Spain
    Nat Cell Biol 6:997-1002. 2004
    ..Here we show that Hog1 regulates cell cycle progression at the G1 phase by a dual mechanism that involves downregulation of cyclin expression and direct targeting of the CDK-inhibitor ..
  36. pmc Phosphorylation of Skp2 regulated by CDK2 and Cdc14B protects it from degradation by APC(Cdh1) in G1 phase
    Geneviève Rodier
    Institut de Recherche en Immunologie et Cancérologie, Universite de Montreal, Montreal, Quebec, Canada
    EMBO J 27:679-91. 2008
    ..Cyclin-dependent kinase (CDK)2-mediated phosphorylation of Skp2 on Ser64 allows its expression in mid-G1 phase, even in the presence of active APC(Cdh1)...
  37. ncbi Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle
    A Koff
    Department of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104
    Science 257:1689-94. 1992
    ..These results provide further evidence that in all eukaryotes assembly of a cyclin-Cdk complex is an important step in the biochemical pathway that controls cell proliferation during G1...
  38. ncbi Cyclin D1 degradation is sufficient to induce G1 cell cycle arrest despite constitutive expression of cyclin E2 in ovarian cancer cells
    Chioniso Patience Masamha
    Departments of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, 73104, USA
    Cancer Res 69:6565-72. 2009
    ..Therefore, cyclin D1 is a sufficient therapeutic target in ovarian cancer cells...
  39. ncbi Inhibition of notch signaling in human embryonic stem cell-derived neural stem cells delays G1/S phase transition and accelerates neuronal differentiation in vitro and in vivo
    Lodovica Borghese
    Institute of Reconstructive Neurobiology, Life and Brain Center, University of Bonn and Hertie Foundation, Bonn, Germany
    Stem Cells 28:955-64. 2010
    ..Thus, interference with Notch signaling provides a tool for controlling human NSC differentiation both in vitro and in vivo...
  40. pmc Assembly in G1 phase and long-term stability are unique intrinsic features of CENP-A nucleosomes
    Dani L Bodor
    Instituto Gulbenkian de Ciencia, 2780 156 Oeiras, Portugal
    Mol Biol Cell 24:923-32. 2013
    ..Previously we showed that newly synthesized CENP-A is targeted to centromeres exclusively during early G1 phase and is subsequently maintained across mitotic divisions...
  41. ncbi A role for the transcription factors Mbp1 and Swi4 in progression from G1 to S phase
    C Koch
    Institute of Molecular Pathology, Vienna, Austria
    Science 261:1551-7. 1993
    ..Mbp1 is related to Swi4. Strains deleted for both MBP1 and SWI4 were inviable, demonstrating that transcriptional activation by MBF and SBF has an important role in the transition from G1 to S phase...
  42. pmc BRCA1-Ku80 protein interaction enhances end-joining fidelity of chromosomal double-strand breaks in the G1 phase of the cell cycle
    Guochun Jiang
    Department of Radiation Oncology, The Ohio State University Medical Center, Arthur G James Comprehensive Cancer Center, Columbus, OH 43210, USA
    J Biol Chem 288:8966-76. 2013
    ..Non-homologous end-joining (NHEJ), a repair process predominant in the G1 phase of the cell cycle, rejoins DSBs either accurately or with errors, but the mechanisms controlling its fidelity are ..
  43. pmc The Yku70-Yku80 complex contributes to regulate double-strand break processing and checkpoint activation during the cell cycle
    Michela Clerici
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milan, Italy
    EMBO Rep 9:810-8. 2008
    ..of any of the NHEJ proteins Yku (Yku70-Yku80), Lif1 or DNA ligase IV (Dnl4) increases 5' DSB end degradation in G1 phase, with ykuDelta cells showing the strongest effect...
  44. ncbi Effect of 5-fluorouracil on G1 phase cell cycle regulation in oral cancer cell lines
    Ming Heng Li
    Second Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Tokyo 145 8515, Japan
    Oral Oncol 40:63-70. 2004
    ..p21 was remarkably up-regulated, but it was speculated that its activity might be cancelled by an increased binding to CDK4...
  45. pmc NF-kappaB function in growth control: regulation of cyclin D1 expression and G0/G1-to-S-phase transition
    M Hinz
    Molekulare Zellbiologie, Humboldt Universitat zu Berlin, Max Delbrück Haus, 13122 Berlin, Germany
    Mol Cell Biol 19:2690-8. 1999
    ..Both levels and kinetics of cyclin D1 expression during G1 phase were controlled by NF-kappaB...
  46. pmc Influenza A virus replication induces cell cycle arrest in G0/G1 phase
    Yuan He
    Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, China
    J Virol 84:12832-40. 2010
    ..These findings, in all, suggest that influenza A virus may provide favorable conditions for viral protein accumulation and virus production by inducing a G(0)/G(1)-phase cell cycle arrest in infected cells...
  47. ncbi Methylselenol, a selenium metabolite, induces cell cycle arrest in G1 phase and apoptosis via the extracellular-regulated kinase 1/2 pathway and other cancer signaling genes
    Huawei Zeng
    USDA, Agricultural Research Service, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58202 9034, USA
    J Nutr 139:1613-8. 2009
    ..The regulation of these genes is likely to play a key role in G1 cell cycle arrest and apoptosis, which may contribute to the inhibition of tumor cell invasion...
  48. ncbi Phosphorylation of Sld2 and Sld3 by cyclin-dependent kinases promotes DNA replication in budding yeast
    Philip Zegerman
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Nature 445:281-5. 2007
    ..DNA replication in cells lacking G1 phase CDK (G1-CDK) required expression of the Cdc7 kinase regulatory subunit, Dbf4, as well as Sld2 and Sld3 bypass...
  49. ncbi An important role for CDK2 in G1 to S checkpoint activation and DNA damage response in human embryonic stem cells
    Irina Neganova
    Institute of Human Genetics, Newcastle University, International Centre for Life, Newcastle upon Tyne, United Kingdom
    Stem Cells 29:651-9. 2011
    ....
  50. ncbi Cell cycle-dependent establishment of a late replication program
    M K Raghuraman
    Department of Genetics, University of Washington, Box 35 7360, Seattle, WA 98195 7360, USA
    Science 276:806-9. 1997
    ..demonstrating that the signal for late activation is established between mitosis and START in the subsequent G1 phase. Once set, the signal can persist through the next S phase in the absence of the telomere...
  51. ncbi Mice lacking p21CIP1/WAF1 undergo normal development, but are defective in G1 checkpoint control
    C Deng
    Howard Hughes Medical Institute, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 82:675-84. 1995
    ..These results establish the role of p21CIP1/WAF1 in the G1 checkpoint, but suggest that the anti-apoptotic and the anti-oncogenic effects of p53 are more complex...
  52. pmc The role of RBF in the introduction of G1 regulation during Drosophila embryogenesis
    W Du
    MGH Cancer Center, Building 149, 13th Street, Charlestown, MA 02129, USA
    EMBO J 18:916-25. 1999
    ..that the repression of E2F target genes by RBF is necessary for the maintenance but not the initiation of a G1 phase. The phenotype of RBF-deficient embryos suggests that rbf has a function that is complementary to the roles of ..
  53. ncbi Molecular mechanism of cell cycle progression induced by the oncogene product Tax of human T-cell leukemia virus type I
    R Iwanaga
    Human Gene Sciences Center, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan
    Oncogene 20:2055-67. 2001
    ..We previously reported that Tax induced cell cycle progression from G0/G1 phase to S and G2/M phases in human T-cell line Kit 225 cells...
  54. pmc Survey of the human pancreatic beta-cell G1/S proteome reveals a potential therapeutic role for cdk-6 and cyclin D1 in enhancing human beta-cell replication and function in vivo
    Nathalie Fiaschi-Taesch
    Division of Endocrinology, The University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Diabetes 58:882-93. 2009
    ....
  55. ncbi Fucoxanthin induces cell cycle arrest at G0/G1 phase in human colon carcinoma cells through up-regulation of p21WAF1/Cip1
    Swadesh K Das
    Department of Life Science, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodai, Kobe, Nada, Hyogo 657 8501, Japan
    Biochim Biophys Acta 1726:328-35. 2005
    ..of WiDr cells in a dose-dependent manner accompanied by the induction of cell cycle arrest during the G0/G1 phase at 25 microM and apoptosis at 50 microM...
  56. pmc Propagation of centromeric chromatin requires exit from mitosis
    Lars E T Jansen
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 176:795-805. 2007
    ..assembly and stabilization of new CENP-A-containing nucleosomes is restricted exclusively to the subsequent G1 phase, demonstrating direct coupling between progression through mitosis and assembly/maturation of the next generation ..
  57. ncbi Hydroxyurea arrests DNA replication by a mechanism that preserves basal dNTP pools
    Ahmet Koc
    Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331, USA
    J Biol Chem 279:223-30. 2004
    ....
  58. ncbi Interdependent nuclear accumulation of budding yeast Cdt1 and Mcm2-7 during G1 phase
    Seiji Tanaka
    Cancer Research UK, Clare Hall Laboratories, Blanche Lane, South Mimms, EN6 3LD, UK
    Nat Cell Biol 4:198-207. 2002
    ..We show that, like Mcm2-7p, Cdt1p accumulates in the nucleus during G1 phase and is excluded from the nucleus later in the cell cycle by cyclin dependent kinases (cdks)...
  59. pmc Silencing of the novel p53 target gene Snk/Plk2 leads to mitotic catastrophe in paclitaxel (taxol)-exposed cells
    Timothy F Burns
    Laboratory of Molecular Oncology and Cell Cycle Regulation, Howard Hughes Medical Institute, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Biol 23:5556-71. 2003
    ..Finally, these studies suggest that disruption of Snk/Plk2 may be of therapeutic value in sensitizing paclitaxel-resistant tumors...
  60. ncbi Hypoxia inhibits G1/S transition through regulation of p27 expression
    L B Gardner
    Department of Medicine, The Johns Hopkins Oncology Center, Baltimore, Maryland 21205, USA
    J Biol Chem 276:7919-26. 2001
    ..This demonstration of the molecular mechanism of hypoxia-induced G1/S regulation provides insight into a fundamental response of mammalian cells to low oxygen tension...
  61. ncbi What's taking so long? S-phase entry from quiescence versus proliferation
    Hilary A Coller
    Department of Molecular Biology, Lewis Thomas Laboratory, Room 140, Princeton University, Princeton, New Jersey 08544 1014, USA
    Nat Rev Mol Cell Biol 8:667-70. 2007
    ..When these cells re-enter the cell cycle, they require several extra hours in the G1 phase before they replicate their DNA compared with their cycling counterparts...
  62. ncbi Gene expression profiling of HeLa cells in G1 or G2 phases
    M Ahmad Chaudhry
    Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, PA 19104, USA
    Oncogene 21:1934-42. 2002
    ..Our analysis identified 154 genes that were elevated in G2 phase of cells as compared to early G1 phase including 15 novel genes. This number included mRNAs whose upregulation is known to occur in G2 phase...
  63. pmc Distinct Cdk1 requirements during single-strand annealing, noncrossover, and crossover recombination
    Camilla Trovesi
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
    PLoS Genet 7:e1002263. 2011
    ..Thus, Cdk1 promotes DSB repair by single-strand annealing and noncrossover recombination by acting mostly at the resection level, whereas additional events require Cdk1-dependent regulation in order to generate crossover outcomes...
  64. ncbi Cell cycle regulation in the G1 phase: a promising target for the development of new chemotherapeutic anticancer agents
    T Owa
    Laboratory of Seeds Finding Technology, Eisai Co, Ltd, 5 1 3 Tokodai, Tsukuba, Ibaraki 300 2635, Japan
    Curr Med Chem 8:1487-503. 2001
    As a result of substantial advances in recent cancer biology, cell cycle regulation in the G1 phase has attracted a great deal of attention as a promising target for the research and treatment of cancer...
  65. pmc Ribosome biogenesis is sensed at the Start cell cycle checkpoint
    Kara A Bernstein
    Department of Genetics, Yale University School of Medicine, New Haven, CT 06520, USA
    Mol Biol Cell 18:953-64. 2007
    ..Thus ribosome biogenesis may be sensed at multiple steps in Start regulation. Ribosome biogenesis may thus both delay Start by increasing the cell size setpoint and independently may promote Start by inactivating Whi5...
  66. ncbi DNA replication licensing and human cell proliferation
    K Stoeber
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    J Cell Sci 114:2027-41. 2001
    ..markers, raising the intriguing possibility that progenitor cells of some tissues are held in a prolonged G1 phase or "in-cycle arrest"...
  67. pmc miR-16 family induces cell cycle arrest by regulating multiple cell cycle genes
    Qin Liu
    Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, People s Republic of China
    Nucleic Acids Res 36:5391-404. 2008
    ..Taken together, our data suggests that miR-16 family triggers an accumulation of cells in G0/G1 by silencing multiple cell cycle genes simultaneously, rather than the individual target...
  68. ncbi Growth inhibition in antimycin A treated-lung cancer Calu-6 cells via inducing a G1 phase arrest and apoptosis
    Yong Hwan Han
    Department of Physiology, Medical School, Institute for Medical Sciences, Chonbuk National University, JeonJu 561 180, Republic of Korea
    Lung Cancer 65:150-60. 2009
    ..AMA inhibited the growth of Calu-6 cells. AMA induced a G1 phase arrest of the cell cycle in these cells at 72h...
  69. ncbi Wagging the dogma; tissue-specific cell cycle control in the mouse embryo
    Michele Pagano
    Department of Pathology and NYU Cancer Institute, New York University School of Medicine, New York, NY 10016, USA
    Cell 118:535-8. 2004
    ....
  70. pmc Mirk/Dyrk1B maintains the viability of quiescent pancreatic cancer cells by reducing levels of reactive oxygen species
    Xiaobing Deng
    Pathology Department, Upstate Medical University, State University of New York, Syracuse, New York 13210, USA
    Cancer Res 69:3317-24. 2009
    ..Thus, quiescent pancreatic cancer cells depleted of Mirk became less viable because they were damaged by ROS, and had increased levels of G(1) cyclins to prime cells to escape quiescence...
  71. pmc Notch1 modulates timing of G1-S progression by inducing SKP2 transcription and p27 Kip1 degradation
    Leonor M Sarmento
    Center of Regenerative Medicine and Technology, Massachusetts General Hospital, Boston, MA, USA
    J Exp Med 202:157-68. 2005
    ....
  72. ncbi FBXO31 is the chromosome 16q24.3 senescence gene, a candidate breast tumor suppressor, and a component of an SCF complex
    Raman Kumar
    Breast Cancer Genetics Group, Dame Roma Mitchell Cancer Research Laboratories, Department of Medicine, University of Adelaide and Hanson Institute, Institute of Medical and Veterinary Science Adelaide, Australia
    Cancer Res 65:11304-13. 2005
    ..We propose that FBXO31 functions as a tumor suppressor by generating SCF(FBXO31) complexes that target particular substrates, critical for the normal execution of the cell cycle, for ubiquitination and subsequent degradation...
  73. ncbi A PHO80-like cyclin and a B-type cyclin control the cell cycle of the procyclic form of Trypanosoma brucei
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 0446, USA
    J Biol Chem 278:20652-8. 2003
    ..This observation indicates an uncoupling between the kinetoplast and the nuclear cycle, resulting in cell division driven by kinetoplast segregation with neither a priori S phase nor mitosis in the trypanosome...
  74. ncbi Cyclic GMP induced apoptosis via protein kinase G in oestrogen receptor-positive and -negative breast cancer cell lines
    Faranak Fallahian
    Department of Clinical Biochemistry, Cancer Research Laboratory, School of Medical Science, Tarbiat Modares University, Tehran, Iran
    FEBS J 278:3360-9. 2011
    ..This study provides direct evidence that the activation of PKG by cGMP induces growth inhibition and apoptosis in MCF-7 and MDA-MB-468 breast cancer cell lines...
  75. ncbi Anticancer mechanisms of YC-1 in human lung cancer cell line, NCI-H226
    Chun Jen Chen
    Graduate Institute of Pharmaceutical Chemistry, China Medical University, No 91, Hsueh Shih Road, Taichung 40421, Taiwan, ROC
    Biochem Pharmacol 75:360-8. 2008
    ..of cyclin D1, CDK2 and cdc25A, up-regulated p16, p21 and p53, increased the number of NCI-H226 cells in the G0/G1 phase of the cell cycle. Long exposure to YC-1 induced apoptosis by mitochondrial-dependent pathway...
  76. ncbi RNA interference-mediated silencing of iASPP induces cell proliferation inhibition and G0/G1 cell cycle arrest in U251 human glioblastoma cells
    Guilin Li
    Department of Neurosurgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, People s Republic of China
    Mol Cell Biochem 350:193-200. 2011
    ..Thus, the findings not only provide a molecular basis for the role of iASPP in cell cycle progression of glioblastoma cells but also suggest a novel therapeutic target for the treatment of GBM...
  77. ncbi Regulation of IGF-1-dependent cyclin D1 and E expression by hEag1 channels in MCF-7 cells: the critical role of hEag1 channels in G1 phase progression
    Anne Sophie Borowiec
    Laboratoire de Physiologie Cellulaire, JE2530, Universite de Picardie Jules Verne, 33 rue St Leu, Amiens, 80039, France
    Biochim Biophys Acta 1813:723-30. 2011
    ..Potassium (K(+)) channels are involved in the G1 phase progression of the cell cycle induced by growth factors...
  78. ncbi Honokiol causes G0-G1 phase cell cycle arrest in human prostate cancer cells in association with suppression of retinoblastoma protein level/phosphorylation and inhibition of E2F1 transcriptional activity
    Eun Ryeong Hahm
    Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Mol Cancer Ther 6:2686-95. 2007
    ..and LNCaP human prostate cancer cells in a concentration- and time-dependent manner, which correlated with G0-G1 phase cell cycle arrest...
  79. ncbi Ectopic expression of cyclin D1 amplifies a retinoic acid-induced mitochondrial death pathway in breast cancer cells
    M Y Niu
    Department of Cellular and Molecular Medicine, University of Ottawa, 451 Smyth Road, Ottawa, Ontario, Canada K1H 8M5
    Oncogene 20:3506-18. 2001
    ..We conclude that constitutive expression of cyclin D1 sensitizes ER-positive breast cancer cells to a retinoic acid-induced mitochondrial death pathway involving Bax activation, cytochrome c release and caspase-9 cleavage...
  80. pmc The transcriptional network activated by Cln3 cyclin at the G1-to-S transition of the yeast cell cycle
    Francisco Ferrezuelo
    Departament de Ciencies Mediques Basiques, Institut de Recerca Biomedica de Lleida, Universitat de Lleida, Montserrat Roig 2, 25008 Lleida, Spain
    Genome Biol 11:R67. 2010
    ..Activation of these factors ultimately depends on the G1 cyclin Cln3...
  81. ncbi Histone deacetylase inhibitors profoundly decrease proliferation of human lymphoid cancer cell lines
    Sakura Sakajiri
    Division of Hematology Oncology, Cedars Sinai Medical Center, UCLA School of Medicine, Los Angeles, CA 90048, USA
    Exp Hematol 33:53-61. 2005
    ..In nude mice, SAHA significantly inhibited growth of a mantle cell lymphoma without major toxic side effects. In summary, HDACIs are promising therapeutic agents for human lymphoid cancers...
  82. pmc Linking DNA replication checkpoint to MBF cell-cycle transcription reveals a distinct class of G1/S genes
    Francisco M Bastos de Oliveira
    Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, USA
    EMBO J 31:1798-810. 2012
    ..Our results reveal that replication stress induces expression of G1/S genes via the Rad53-MBF pathway and that an SBF-to-MBF switch characterizes a new class of genes that can be induced by replication stress...
  83. ncbi Endoplasmic reticulum chaperone protein GRP78 protects cells from apoptosis induced by topoisomerase inhibitors: role of ATP binding site in suppression of caspase-7 activation
    Ramachandra K Reddy
    Department of Biochemistry and Molecular Biology and the USC Norris Comprehensive Cancer Center, University of Southern California Keck School of Medicine, Los Angeles, California 90089 9176, USA
    J Biol Chem 278:20915-24. 2003
    ..Lastly, a GRP78 mutant deleted of its ATP binding domain fails to bind procaspase-7 and loses its protective effect against etoposide-induced apoptosis...
  84. pmc Reducing MCM levels in human primary T cells during the G(0)-->G(1) transition causes genomic instability during the first cell cycle
    S J Orr
    King s College London, Department of Haematological Medicine, Leukaemia Sciences Laboratories, Rayne Institute, London, UK
    Oncogene 29:3803-14. 2010
    ..Therefore, a simple reduction in MCM loading onto DNA, which occurs in cancers as a result of aberrant cell cycle control, is sufficient to cause PCS and gross genomic instability within one cell cycle...
  85. pmc c-ETS1 facilitates G1/S-phase transition by up-regulating cyclin E and CDK2 genes and cooperates with hepatitis B virus X protein for their deregulation
    Anup Kumar Singh
    Virology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India
    J Biol Chem 286:21961-70. 2011
    ..Thus, cyclin E and CDK2 genes are key physiological effectors of the c-ETS1 proto-oncogene. Furthermore, c-ETS1 is indispensable for the hepatotropic action of HBx in cell cycle deregulation...
  86. ncbi Inhibition of heat shock protein 90 function by ansamycins causes the morphological and functional differentiation of breast cancer cells
    P N Munster
    Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Cancer Res 61:2945-52. 2001
    ..G1 arrest is sufficient for some but not all aspects of the phenotype. Induction of differentiation may be responsible for some of the antitumor effects of this drug...
  87. pmc A truncated DNA-damage-signaling response is activated after DSB formation in the G1 phase of Saccharomyces cerevisiae
    Ryan Janke
    Department of Microbiology, University of California, Davis, CA 95616 8665, USA
    Nucleic Acids Res 38:2302-13. 2010
    ..These data suggest the existence of a truncated signaling pathway in response to a single DSB in G1-arrested cells that activates Mec1 without eliciting a full DDR involving the entire signaling pathway including the effector kinases...
  88. ncbi Down-regulation of Notch-1 contributes to cell growth inhibition and apoptosis in pancreatic cancer cells
    Zhiwei Wang
    Department of Pathology, Karmanos Cancer Institute, Wayne State University School of Medicine, 9374 Scott Hall, 540 East Canfield, Detroit, MI 48201, USA
    Mol Cancer Ther 5:483-93. 2006
    ..These findings suggest that Notch-1 down-regulation, especially by genistein, could be a novel therapeutic approach for the treatment of pancreatic cancer...
  89. ncbi The ubiquitin ligase APC(Cdh1) is required to maintain genome integrity in primary human cells
    D Engelbert
    Department of Hematology and Oncology, Albert Ludwigs University Medical Center, Freiburg, Germany
    Oncogene 27:907-17. 2008
    ..cycle regulators by the anaphase-promoting complex, activated by Cdh1 (APC(Cdh1)), is responsible for a stable G1 phase after mitotic exit allowing accurate preparation for DNA replication in the following S phase...
  90. ncbi Cyclin D1 is transcriptionally regulated by and required for transformation by activated signal transducer and activator of transcription 3
    Kenneth Leslie
    Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Cancer Res 66:2544-52. 2006
    ..These results suggest both a cdk-dependent and cdk-independent role for cyclin D1 in modulating transformation by different oncogenes...
  91. ncbi Underlying principles of cell fate determination during G1 phase of the mammalian cell cycle
    Benjamin Pfeuty
    Department of Pure and Applied Sciences, University of Tokyo, Tokyo, Japan
    Cell Cycle 7:3246-57. 2008
    ..The final outcome ultimately depends on the context-dependent coordination between the cell-growth and cell-division processes...
  92. ncbi Reappraisal of serum starvation, the restriction point, G0, and G1 phase arrest points
    Stephen Cooper
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
    FASEB J 17:333-40. 2003
    The restriction point in the G1 phase of the mammalian cell cycle is the oldest, best-known, and widely accepted control point regulating division cycle in mammalian cells...
  93. ncbi Dynamics of DNA double-strand breaks revealed by clustering of damaged chromosome domains
    Jacob A Aten
    Center for Microscopical Research, Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, P O Box 22700, 1100 DE Amsterdam, Netherlands
    Science 303:92-5. 2004
    ..Juxtaposition of different DSB-containing chromosome domains through clustering, which was most extensive in G1 phase cells, suggests an adhesion process in which we implicate the Mre11 complex...
  94. pmc Mammalian Cdh1/Fzr mediates its own degradation
    Tamar Listovsky
    Department of Genetics, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel
    EMBO J 23:1619-26. 2004
    ..This auto-regulation of Cdh1 could thus play a role in ensuring that the level of Cdh1 is reduced during G1 and G0, allowing it to be switched off at the correct time...
  95. pmc RACK1 regulates G1/S progression by suppressing Src kinase activity
    Vidya Mamidipudi
    Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305 5187, USA
    Mol Cell Biol 24:6788-98. 2004
    ..We also show that Stat3 is required for Rac1 induction of Myc. Our results reveal a novel mechanism of cell cycle control in late G(1) that works via an endogenous inhibitor of the Src kinase...
  96. ncbi The Yin Yang-1 (YY1) protein undergoes a DNA-replication-associated switch in localization from the cytoplasm to the nucleus at the onset of S phase
    Linda Palko
    Department of Biological Science, Florida State University, Tallahassee, Florida 32306 4370, USA
    J Cell Sci 117:465-76. 2004
    ..Taken together, our results provide insight into how YY1 might participate in the cell-cycle control over a variety of nuclear events required for cell division and proliferation...
  97. ncbi Nuclear matrix support of DNA replication
    Boyka Anachkova
    Institute of Molecular Biology, Bulgarian Academy of Sciences, Akad G Bonchev Street, Bl 21, Sofia 1113, Bulgaria
    J Cell Biochem 96:951-61. 2005
    ..During late G1 phase, the replication origins are associated with the nuclear matrix and dissociated after initiation of replication ..
  98. ncbi Restriction point control of the mammalian cell cycle via the cyclin E/Cdk2:p27 complex
    Riaan Conradie
    Triple J Group for Molecular Cell Physiology, Department of Biochemistry, Stellenbosch University, Matieland, South Africa
    FEBS J 277:357-67. 2010
    ....
  99. pmc Formation of dynamic gamma-H2AX domains along broken DNA strands is distinctly regulated by ATM and MDC1 and dependent upon H2AX densities in chromatin
    Velibor Savic
    Cell and Molecular Biology Graduate Program, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Mol Cell 34:298-310. 2009
    ..demonstrate that gamma-H2AX densities increase transiently along DNA strands as they are broken and repaired in G1 phase cells...
  100. ncbi The limitations of the G1-S checkpoint
    Dorothee Deckbar
    Radiation Biology and DNA Repair, Darmstadt University of Technology, Darmstadt, Germany
    Cancer Res 70:4412-21. 2010
    ....
  101. ncbi APC/CCdh1 specific degradation of Hsl1 and Clb2 is required for proper stress responses of S. cerevisiae
    Kobi J Simpson-Lavy
    The Department of Genetics, The Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
    Cell Cycle 8:3003-9. 2009
    ..We thus hypothesize that a role of G(1) specific APC/C(Cdh1) activity is to reset substrate levels to enables appropriate regulation of substrate accumulation in the subsequent phases of the cell cycle...

Research Grants67

  1. Modulation of Glucocorticoid-Evoked Lymphocyte Apoptosis by BTG1
    RHEEM MEDH; Fiscal Year: 2007
    ..Chemotherapeutic agents such as glucocorticoids (GCs), used in T cell leukemias, arrest cells in G1 phase of the cell cycle and induce apoptosis by altering expression of key growth regulatory/death inducing genes...
  2. Using Mass Spectroscopy to Characterize Transcription Proteins and F2-Isoprostane
    BETTYE HENNINTON; Fiscal Year: 2011
    ..To test the hypothesis that phosphorylation is either increased or decreased on Gcrlp when cells are arrested at G1 phase of the cell cycle and at G2 phase of the cell cycle...
  3. ECM compliance and cell cycle control
    RICHARD ASSOIAN; Fiscal Year: 2012
    ..The necessity of integrin signaling for G1 phase cell cycle progression is now well established, but the approaches that have been used to document ECM/integrin ..
  4. Role of Non-Core RAG1 in DSB Repair, Cell Survival, and Cell Cycle Progression
    Julie Horowitz; Fiscal Year: 2013
    ..provided by applicant): The RAG1/RAG2 endonuclease catalyzes assembly of antigen receptor genes in developing G1 phase lymphocytes through the induction of DNA double-strand breaks (DSBs), which are repaired by non- homologous end-..
  5. Physiologic Regulation of Hematopoiesis by Notch
    Nadia Carlesso; Fiscal Year: 2011
    ..preserving them in a more immature phenotype, and induces perturbation of their cell cycle by shortening their G1 phase and promoting premature entry into S-phase...
  6. Control of the Erythroid Terminal Differentiation Decision
    Arthur I Skoultchi; Fiscal Year: 2013
    ..1. We also showed that: 1) CDK6 is the active G1 phase D-cyclin kinase in erythroid progenitors;2) PU...
  7. Replication licensing and cell cycle checkpoints
    Jeanette Gowen Cook; Fiscal Year: 2013
    ..Origin licensing is accomplished through the assembly of prereplication complexes at origins during the G1 phase of the cell cycle...
  8. Role of ORCA in DNA replication
    Supriya Gangadharan Prasanth; Fiscal Year: 2013
    ..from human cells results in reduced binding of ORC proteins to chromatin and subsequent accumulation of cells in G1 phase. However, the roles of ORCA in preRC assembly and in stabilizing ORC to chromatin remain to be elucidated...
  9. Role of Cyclins in Brain Construction and Interneuron Genesis
    KRISTIN GIAMANCO; Fiscal Year: 2013
    ..These studies will contribute to the ultimate goal to devise more effective therapies for neuropsychiatric disorders. ..
  10. Targeting Src Signaling Pathways to Promote Cell Cycle Arrest in Ovarian Cancer
    Fiona Simpkins; Fiscal Year: 2013
    ..E2 deprivation and estrogen receptor(ER) blockade because breast cancer (BRCA) cells to arrest in the G1 phase of the cell cycle16, 17...
  11. Linking DNA Replication Origin Licensing with Cell Cycle Progression
    Kate E Coleman; Fiscal Year: 2013
    ..S phase by the chromatin-loading of a DNA helicase known as the Mini-Chromosome Maintenance Complex (MCM) during G1 phase of the cell cycle...
  12. REGULATION OF THE DEVELOPMENT OF TRYPANOSOMA BRUCEI
    CHING CHUNG WANG; Fiscal Year: 2012
    ..mitotic arrest in the procylic form but not in the bloodstream form, which continues with re-entries into a new G1 phase and nonstop nuclear DNA synthesis...
  13. MECHANISM OF IMMUNOGLOBULIN HYPERMUTATION
    URSULA B STORB; Fiscal Year: 2012
    ..Most of the experiments will at first be carried out in B cell lines in culture that undergo somatic hypermutation. If conclusive in cultured cells, some experiments will be checked in normal and transgenic mice. ..
  14. Genome wide analysis of p53 inhibition by high glucose
    Selene Bobadilla; Fiscal Year: 2013
    ..subunit of transcription factor TFIID and a cell cycle regulatory protein important for progression through the G1 phase. Interestingly, we also uncovered that TAF1 phosphorylates p53 in a cellular ATP level-dependent manner...
  15. REGULATION OF CYCLIN D1 EXPRESSION IN THE INTESTINE
    Jennifer D Black; Fiscal Year: 2013
    ..sensor of extracellular growth stimulatory and inhibitory signals and a key determinant of progression through G1 phase. Aberrant expression of cyclin D1 is one of the most frequent abnormalities in human cancer, and is believed to ..
  16. Optimizing Wolbachia manipulation in mosquito cell lines
    Ann M Fallon; Fiscal Year: 2013
    ..life cycle is coordinated with the mosquito cell cycle, and that Wolbachia genome replication occurs during the G1 phase of the mitotic cycle...
  17. Cell Cycle Regulation of Yeast Telomerase Assembly and Function
    KATHERINE LOUISE FRIEDMAN; Fiscal Year: 2012
    ..Previous work from the P.I.'s laboratory demonstrated that the regulated degradation of Est1p during G1 phase prevents assembly of the telomerase complex...
  18. REGULATION OF G1-SPECIFIC GENE EXPRESSION IN YEAST
    Curt Wittenberg; Fiscal Year: 2013
    ..Greater than 300 genes are coordinately expressed during late G1 phase in preparation for entry into a new cell cycle...
  19. Investigating the Role of the Est1p in Telomerase Biogenesis and Regulation
    Charlene Hawkins; Fiscal Year: 2012
    ..of telomerase assembly and function: its cell cycle-regulated degradation precludes telomerase assembly in G1 phase of the yeast cell cycle;it likely interacts directly with both the TLC1 RNA and with Est2p;it stimulates the ..
  20. Plk1 in Chemo-resistance of Cancer
    Xiaoqi Liu; Fiscal Year: 2013
    ..Association of p21 with the GTSE1/Hsp90/WISp39 complex in G2 phase and the Sgt1/Hsp90 complex in G1 phase protects it from degradation by the proteasome...
  21. REGULATION OF THE DEVELOPMENT OF TRYPANOSOMA BRUCEI
    Ching Wang; Fiscal Year: 2009
    ..procylic form but not in the bloodstream form, which continues with reform, The bloodstream entries into a new G1 phase and nonstop nuclear DNA synthesis...
  22. Organismal Radioprotection Through Pharmacological Quiescence
    JAY COPELAND STRUM; Fiscal Year: 2013
    ..of inhibitors for acute induction of PQ (a 4-fold or greater increase in the frequency of HSPC in the G0/G1 phase of the cell cycle at 24 hours post-gavage without increased cell death) will serve as a pharmacodynamic (PD) ..
  23. REGULATION OF CYCLIN D1 EXPRESSION IN THE INTESTINE
    Jennifer Black; Fiscal Year: 2009
    ..sensor of extracellular growth stimulatory and inhibitory signals and a key determinant of progression through G1 phase. Aberrant expression of cyclin D1 is one of the most frequent abnormalities in human cancer, and is believed to ..
  24. NUCKS, a novel double-strand break repair gene, implicated in cancer biology
    Claudia Wiese; Fiscal Year: 2013
    ..In addition, our studies may also contribute to the improved diagnosis, prevention and treatment of cancer. ..
  25. Role of TRIP6 in Malignant Glioma Progression
    Fang Tsyr Lin; Fiscal Year: 2010
    ..data showing that inhibition of TRIP6 expression reduces cell migration, enhances chemosensitivity and prolongs G1 phase of the cell cycle in glioblastoma multiforme cells, suggesting a critical role for TRIP6 in malignant glioma ..
  26. p53 Acetylation and Deacetylation in Tumorigenesis
    Wei Gu; Fiscal Year: 2012
    ..of the genome because it responds to DNA damage or other types of stress by either arresting the cell in the G1 phase for damage repair or initiating an apoptotic pathway to eliminate the damaged cell entirely...
  27. Function of the AID C terminus in Ig class switching
    JANET M STAVNEZER; Fiscal Year: 2013
    ..protein, and (2) that the C terminus of AID is important for recruiting UNG and Msh2-Msh6 to S regions during the G1 phase of the cell cycle, and (3) that the C terminus of AID is important for steps subsequent to formation of DNA ..
  28. Role of p53 in cell cycle checkpoints
    James J Manfredi; Fiscal Year: 2013
    ..p53 has a well-characterized role in mediating the cellular response to DNA damage. The checkpoint in the G1 phase has been shown to be strictly p53-dependent...
  29. Cyclin-Dependent Kinases in Oncogenesis
    Peter Sicinski; Fiscal Year: 2013
    ..Of particular importance for the cancer field are cyclin-CDK complexes which operate during the G1 phase of the cell cycle...
  30. Mip/LIN9 and Cell Proliferation
    Oscar R Colamonici; Fiscal Year: 2012
    The G1 phase of the cell cycle is the target of extracellular signals that regulate cell proliferation...
  31. Small RNAs in Stem Cell Differentiation and Neural Development
    Robert H Blelloch; Fiscal Year: 2010
    ..in stem cell proliferation based on preliminary results showing that DGCR8 null ES cells accumulate in the G1 phase of the cell cycle, 2) characterization of the role of miRNAs in stem cell differentiation based on initial ..
  32. Regulation of p53 by TAF1
    Xuan Liu; Fiscal Year: 2012
    ..of transcription factor TFIID and is also a cell cycle regulatory protein important for progression through the G1 phase. Thr55 phosphorylation contributes to TAF1-induced cell G1 progression...
  33. Backup origins: their role in replication fork recovery and tumor suppression
    Naoko Shima; Fiscal Year: 2013
    ..During the G1 phase, replication origins are licensed by the binding of MCM2-7 complexes, the replicative helicases, and are ..
  34. Transcriptional Networks Controlling Erythroid Differentiation
    Gerd A Blobel; Fiscal Year: 2013
    ..the appropriated re-assembly transcription factor complexes at the correct sites upon re-entry into the G1 phase of the cell cycle...
  35. "Oncorequisite" Genes in MYC-mediated Transformation
    Hui Feng; Fiscal Year: 2013
    ..Tumor cells with 50% reduction of Dlst tended to be in G1 phase and morphologically more differentiated with a stable genome...
  36. High throughput monitoring of mass, density and fluorescence of single cells
    Scott R Manalis; Fiscal Year: 2011
    ..The relationship between cell growth and G1 phase is central to understanding the cell cycle and has important ramifications for oncology...
  37. Markers of sensivity to the EGFR inhibitor erlotinib in breast cancer
    Naoto T Ueno; Fiscal Year: 2012
    ..kinase 2 (Cdk2), which is the most downstream kinase of the EGFR pathway that regulates the transition from the G1 phase to the S phase...
  38. Screen and Design p18 Chemical Probes for Hematopoietic Stem Cell Self-Renewal
    Xiang Qun Xie; Fiscal Year: 2012
    ..Achieving this goal will have a significant impact on stem cell drug research development in general. ..
  39. Dynamics and evolution of cell cycle transitions
    Jan M Skotheim; Fiscal Year: 2013
    ..Information about pheromone concentration is integrated in G1 phase, prior to the 'Start'of the cell cycle...
  40. The Role of pRB and Co-repressors in Transcriptional Regulation
    Brian D Dynlacht; Fiscal Year: 2013
    ..It is also unclear what transcriptional controls distinguish cells progressing through the M/G1 phase transition from those observed in cells re-emerging from growth arrest (the G0/G1 phase transition) and how ..
  41. MENTORED RESEARCH SCIENTIST DEVELOPMENT AWARD
    Baktiar O Karim; Fiscal Year: 2010
    ..Cyclin dependent kinase 4 (Cdk4) regulates the re-entry of cells into the G1 phase of the cell cycle...
  42. Phospholipase D-mTOR Survival Signals in Tumorigenesis
    David A Foster; Fiscal Year: 2013
    ..and mTOR in human cancer cells and the impact of these signals on a proposed "Cell Growth Checkpoint" in the G1 phase of the cell cycle that we are proposing must be overcome in virtually all human cancers...
  43. Development of New therapies for Advanced Prostate Cancer
    Kimberly M Jackson; Fiscal Year: 2011
    ..that DBM inhibits the proliferation of prostate cancer cells in vitro along with an accumulation of cells in the G1 phase of the cell cycle. The mechanistic basis for these antiproliferative effects are unknown...
  44. Mechanism of the eukaryotic replicative DNA helicase
    Johannes Walter; Fiscal Year: 2013
    ..However, the MCM2-7 complex may not fit this simple paradigm. In the G1 phase of the cell cycle, MCM2-7 is recruited into pre-Replication Complexes (pre-RCs) as numerous inactive dimers that ..
  45. Pathways that integrate cell growth with the cell cycle
    Douglas R Kellogg; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): A critical point in the cell cycle occurs in G1 phase, when cells must decide whether to enter a new round of cell division...
  46. MOLECULAR REGULATION OF LIVER REGENERATION
    Clifford Steer; Fiscal Year: 1999
    ..The proposed studies will provide important and new information in our understanding of cell growth and the control of gene expression in the ability of the liver to recover from injury. ..
  47. Alteration of Rb in HTLV-1 transformed cells
    Fatah Kashanchi; Fiscal Year: 2005
    ..This concerted deregulation, especially at the G1 phase of the cell cycle, ensures continuous cellular progression via the loss of checkpoint control and insensitivity ..
  48. The Role of Cell Size and G1-phase Cdks in Cell Growth Control
    Brandt L Schneider; Fiscal Year: 2010
    ..This exemplifies the critical importance of understanding the role of G1-phase Cdks in proliferation because this type of information is essential before effective anti-cancer clinical interventions can be designed. ..
  49. Mouse Models of ErbB-2 and Cyclin D1 in Prostate Cancer
    Christopher Albanese; Fiscal Year: 2002
    ..We and others have shown that the abundance of cyclin D1 is rate-limiting in progression through the G1 phase of the cell-cycle and is required for transformation by Ras and ErbB-2 in fibroblast assays...
  50. CYCLIN D1 AND CELL ADHESION
    RICHARD ASSOIAN; Fiscal Year: 2007
    Cyclin D1 is an important regulator of cell cycle progression through G1 phase. Although initially considered to be strictly a target of growth factor stimulation, we and now others have shown that the induction of cyclin D 1 actually ..
  51. CDK Inhibitor and B Cell Terminal Differentiation
    Selina Chen Kiang; Fiscal Year: 2006
    ..It will also provide a framework for future studies of cell cycle control in B cell immunity, the formation of memory B cells in particular, as well as cell cycle dysregulation in B cell and plasma cell tumorigenesis. ..
  52. Interaction of statins with the proteasome/breast cancer
    Ekem Efuet; Fiscal Year: 2009
    ..We show that upon treating cells with Lovastatin, they arrest in the G1 phase of the cell cycle, as indicated by FACS analysis...
  53. MECHANISM OF CIGARETTE SMOKE-INDUCED IMMUNOSUPPRESSION
    Mohan Sopori; Fiscal Year: 2003
    ..to demonstrate that chronic exposure to NT causes immunosuppression similar to SM and arrests T cells in the G0/G1 phase of the cell cycle...
  54. Genetic Basis of Lung Tumor Progression in Mice
    Ming You; Fiscal Year: 2006
    ..INK4 (Inhibitors of CDK4) family of cyclin-dependent kinase inhibitors that controls cellular transit through the G1 phase of the cell cycle by inhibiting the D-type cyclin-dependent kinases (CDK4 and CDK6)...
  55. Immunotoxicology of Cigarette Smoke
    Brian Freed; Fiscal Year: 2007
    ..However, they inhibit normal entry and progression through the G1 phase of the cell cycle...
  56. REGULATION OF CYCLIN/CDK ACTIVITY IN YEAST
    Raymond Deshaies; Fiscal Year: 2000
    In most eukaryotic cells the decision to enter into the cell division cycle is made during the G1 phase. A recent confluence of many different lines of research in a variety of organisms has shown that the transient accumulation of active ..
  57. DEVELOPING VISION--THE MECHANISM OF TISSUE REMODELING
    Richard Lang; Fiscal Year: 2003
    ..abstract_text> ..
  58. REGULATION OF CELL STRUCTURE AND GROWTH BY E2F1
    David Hall; Fiscal Year: 2000
    ..E2F1 is normally expressed at the end of GO/G1 phase and regulates genes whose products are needed for movement through S phase...
  59. PROTEIN PHOSPHATASE 1 AND NEOPLASTIC TRNSFORMATION
    Norbert Berndt; Fiscal Year: 2004
    ..In Aim 3, the previous results will be tested by asking the question whether oncogene mediated immortalization and transformation is associated with changes affecting PP1's ability to maintain active RB. ..
  60. ONCOGENE MEDIATED CHANGES IN CELL CYCLE CONTROL
    Edward Harlow; Fiscal Year: 2000
    ..For mammalian cells most of the key regulatory events that control commitment to cell division occur in the G1 phase of the division cycle. There are two G1 pathways in which cdk's participate...
  61. P53 TRAFFICKING DURING CELL CYCLE
    Michael Clarke; Fiscal Year: 2000
    ..This and other laboratories has shown that during the G1 phase of the cell cycle p53 can induce apoptosis in some cell lines and cell cycle arrest in others...
  62. Chemoprevention of Photocarcinogenesis by Resveratrol
    Nihal Ahmad; Fiscal Year: 2003
    ..This may pave the way for the development of novel strategies for prevention and possibly treatment of skin cancer and other epithelial cancers as well. ..
  63. Mechanism of PCNA-Dependent Cdt1 Destruction in S phase
    COURTNEY HAVENS; Fiscal Year: 2009
    ..After initiation, origins cannot be re-licensed until the following G1 phase due to inhibition of Cdt1 activity through Geminin and ubiquitin-mediated proteolysis of Cdt1...
  64. LSF AND MAMMALIAN CELL CYCLE PROGRESSION
    Ulla Hansen; Fiscal Year: 2004
    ..At one pivotal point in late G1 phase, the restriction point, cellular signals are integrated to determine whether to replicate cellular DNA (progress ..
  65. TWO NOVEL CPLA2 BINDING PROTEINS AND CELL DEATH
    Alice Sheridan; Fiscal Year: 2005
    ..We have recently demonstrated that PLIP expression is associated with a marked decrease in GO/G1 phase cells associated with a decrease in retinoblastoma protein...
  66. CHARACTERIZATION OF THE CELL CYCLE REGULATED GENE HG1.1
    Richard Bird; Fiscal Year: 1992
    ..One current model of cell cycle regulation holds that G1 phase represents a gateway during which cells either cease to proliferate before retiring from the cell cycle to ..
  67. GROWTH CONTROL IN AGING FIBROBLASTS
    Eugenia Wang; Fiscal Year: 2003
    ..During early G1 phase, rapid statin loss releases p45 kinase to phosphorylate RB; in nongrowing cells such as senescent fibroblasts, ..