erythropoiesis

Summary

Summary: The production of red blood cells (ERYTHROCYTES). In humans, erythrocytes are produced by the YOLK SAC in the first trimester; by the liver in the second trimester; by the BONE MARROW in the third trimester and after birth. In normal individuals, the erythrocyte count in the peripheral blood remains relatively constant implying a balance between the rate of erythrocyte production and rate of destruction.

Top Publications

  1. ncbi Ontogeny of erythropoiesis
    James Palis
    Department of Pediatrics, University of Rochester Medical Center, Rochester, New York 14642, USA
    Curr Opin Hematol 15:155-61. 2008
  2. pmc From stem cell to red cell: regulation of erythropoiesis at multiple levels by multiple proteins, RNAs, and chromatin modifications
    Shilpa M Hattangadi
    Whitehead Institute for Biomedical Research, Cambridge, MA, USA
    Blood 118:6258-68. 2011
  3. ncbi Erythropoietin, erythropoiesis and beyond
    S Chateauvieux
    Laboratoire de Biologie Moléculaire et Cellulaire du Cancer, Hopital Kirchberg, 9, rue Edward Steichen, L 2540 Luxembourg, Luxembourg
    Biochem Pharmacol 82:1291-303. 2011
  4. ncbi Erythropoiesis failure due to RPS19 deficiency is independent of an activated Tp53 response in a zebrafish model of Diamond-Blackfan anaemia
    Hidetsugu Torihara
    Frontier Science Research Centre, University of Miyazaki, Kiyotake, Miyazaki, Japan
    Br J Haematol 152:648-54. 2011
  5. pmc The miR-144/451 locus is required for erythroid homeostasis
    Kasper D Rasmussen
    European Molecular Biology Laboratory, Mouse Biology Unit, Monterotondo Scalo, 00015, Italy
    J Exp Med 207:1351-8. 2010
  6. ncbi Ribosomal protein S19 deficiency in zebrafish leads to developmental abnormalities and defective erythropoiesis through activation of p53 protein family
    Nadia Danilova
    Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, UCLA, USA
    Blood 112:5228-37. 2008
  7. pmc NRF2, a member of the NFE2 family of transcription factors, is not essential for murine erythropoiesis, growth, and development
    K Chan
    Institute of Molecular Biology, University of Hong Kong, Hong Kong
    Proc Natl Acad Sci U S A 93:13943-8. 1996
  8. ncbi Detection, evaluation, and management of iron-restricted erythropoiesis
    Lawrence Tim Goodnough
    Departments of Pathology and Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
    Blood 116:4754-61. 2010
  9. ncbi Erythropoietin stimulates spleen BMP4-dependent stress erythropoiesis and partially corrects anemia in a mouse model of generalized inflammation
    Sarah Millot
    INSERM U773, Centre de recherche Biomedicale Bichat Beaujon, Universite Paris Diderot, Paris, France
    Blood 116:6072-81. 2010
  10. ncbi Human fetal hemoglobin expression is regulated by the developmental stage-specific repressor BCL11A
    Vijay G Sankaran
    Division of Hematology Oncology, Children s Hospital Boston, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA
    Science 322:1839-42. 2008

Detail Information

Publications328 found, 100 shown here

  1. ncbi Ontogeny of erythropoiesis
    James Palis
    Department of Pediatrics, University of Rochester Medical Center, Rochester, New York 14642, USA
    Curr Opin Hematol 15:155-61. 2008
    The present study review examines the current understanding of the ontogeny of erythropoiesis with a focus on the emergence of the embryonic (primitive) erythroid lineage and on the similarities and differences between the primitive and ..
  2. pmc From stem cell to red cell: regulation of erythropoiesis at multiple levels by multiple proteins, RNAs, and chromatin modifications
    Shilpa M Hattangadi
    Whitehead Institute for Biomedical Research, Cambridge, MA, USA
    Blood 118:6258-68. 2011
    ..We discuss the complex intracellular networks of coordinated gene regulation by transcription factors, chromatin modifiers, and miRNAs that regulate the different stages of erythropoiesis.
  3. ncbi Erythropoietin, erythropoiesis and beyond
    S Chateauvieux
    Laboratoire de Biologie Moléculaire et Cellulaire du Cancer, Hopital Kirchberg, 9, rue Edward Steichen, L 2540 Luxembourg, Luxembourg
    Biochem Pharmacol 82:1291-303. 2011
    ..The low affinity of the alternative receptor for EPO involves the injection of excessive concentration of erythropoiesis-stimulating agents (ESAs), implicating side effects due to the cross-talk with hematopoietic activity...
  4. ncbi Erythropoiesis failure due to RPS19 deficiency is independent of an activated Tp53 response in a zebrafish model of Diamond-Blackfan anaemia
    Hidetsugu Torihara
    Frontier Science Research Centre, University of Miyazaki, Kiyotake, Miyazaki, Japan
    Br J Haematol 152:648-54. 2011
    ..development, but it is not clear why mutations in the ubiquitously expressed RPS19 gene specifically affect erythropoiesis. Previously, we showed that RPS19 deficiency in zebrafish recapitulates the erythropoietic and developmental ..
  5. pmc The miR-144/451 locus is required for erythroid homeostasis
    Kasper D Rasmussen
    European Molecular Biology Laboratory, Mouse Biology Unit, Monterotondo Scalo, 00015, Italy
    J Exp Med 207:1351-8. 2010
    The process of erythropoiesis must be efficient and robust to supply the organism with red bloods cells both under condition of homeostasis and stress. The microRNA (miRNA) pathway was recently shown to regulate erythroid development...
  6. ncbi Ribosomal protein S19 deficiency in zebrafish leads to developmental abnormalities and defective erythropoiesis through activation of p53 protein family
    Nadia Danilova
    Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, UCLA, USA
    Blood 112:5228-37. 2008
    ..several ribosomal proteins (RPs) lead to Diamond-Blackfan anemia (DBA), a syndrome characterized by defective erythropoiesis, congenital anomalies, and increased frequency of cancer. RPS19 is the most frequently mutated RP in DBA...
  7. pmc NRF2, a member of the NFE2 family of transcription factors, is not essential for murine erythropoiesis, growth, and development
    K Chan
    Institute of Molecular Biology, University of Hong Kong, Hong Kong
    Proc Natl Acad Sci U S A 93:13943-8. 1996
    ..Homozygous mutant mice developed normally, were not anemic, reached adulthood, and reproduced. Our studies indicate that NRF2 is dispensable for mouse development...
  8. ncbi Detection, evaluation, and management of iron-restricted erythropoiesis
    Lawrence Tim Goodnough
    Departments of Pathology and Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
    Blood 116:4754-61. 2010
    Progress in our understanding of iron-restricted erythropoiesis has been made possible by important advances in defining the molecular mechanisms of iron homeostasis...
  9. ncbi Erythropoietin stimulates spleen BMP4-dependent stress erythropoiesis and partially corrects anemia in a mouse model of generalized inflammation
    Sarah Millot
    INSERM U773, Centre de recherche Biomedicale Bichat Beaujon, Universite Paris Diderot, Paris, France
    Blood 116:6072-81. 2010
    Mouse bone marrow erythropoiesis is homeostatic, whereas after acute anemia, bone morphogenetic protein 4 (BMP4)-dependent stress erythropoiesis develops in the spleen...
  10. ncbi Human fetal hemoglobin expression is regulated by the developmental stage-specific repressor BCL11A
    Vijay G Sankaran
    Division of Hematology Oncology, Children s Hospital Boston, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA
    Science 322:1839-42. 2008
    ..BCL11A emerges as a therapeutic target for reactivation of HbF in beta-hemoglobin disorders...
  11. pmc Stress erythropoiesis: new signals and new stress progenitor cells
    Robert F Paulson
    Department of Veterinary and Biomedical Sciences, Center for Molecular Immunology and Infectious Disease, The Pennsylvania State University, University Park, Pennsylvania, USA
    Curr Opin Hematol 18:139-45. 2011
    ..This process is referred to as stress erythropoiesis, which is distinct from steady state erythropoiesis...
  12. ncbi Red cell distribution width in heart failure: prediction of clinical events and relationship with markers of ineffective erythropoiesis, inflammation, renal function, and nutritional state
    Zsolt Förhécz
    3rd Department of Internal Medicine, and Szentágothai Knowledge Center, Semmelweis University, Budapest, Hungary
    Am Heart J 158:659-66. 2009
    ..Understanding the mechanism underlying this observation is unclear, largely hampered by the lack of epidemiologic studies demonstrating factors that are associated with anisocytosis in cardiovascular diseases...
  13. pmc Transcriptional silencing of {gamma}-globin by BCL11A involves long-range interactions and cooperation with SOX6
    Jian Xu
    Children s Hospital Boston, Massachusetts 02115, USA
    Genes Dev 24:783-98. 2010
    ..Our findings provide insight into the mechanism of BCL11A action and new clues for the developmental gene regulatory programs that function at the beta-globin locus...
  14. pmc Genome-wide identification of TAL1's functional targets: insights into its mechanisms of action in primary erythroid cells
    Mira T Kassouf
    MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford University, Oxford OX3 9DS, United Kingdom
    Genome Res 20:1064-83. 2010
    ..To gain insight into TAL1 function and mechanisms of action in erythropoiesis, we performed ChIP-sequencing and gene expression analyses from primary fetal liver erythroid cells...
  15. pmc Global DNA demethylation during mouse erythropoiesis in vivo
    Jeffrey R Shearstone
    Department of Pediatrics, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 334:799-802. 2011
    ..Hence, DNA demethylation can occur globally during somatic cell differentiation, providing an experimental model for its study in development and disease...
  16. pmc A Slicer-independent role for Argonaute 2 in hematopoiesis and the microRNA pathway
    Dónal O'Carroll
    The Laboratory for Lymphocyte Signaling, The Rockefeller University, New York, New York 10021, USA
    Genes Dev 21:1999-2004. 2007
    ..Collectively, our data identify Ago2 as a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway...
  17. ncbi Generation of lymphohematopoietic cells from embryonic stem cells in culture
    T Nakano
    Department of Medical Chemistry, Faculty of Medicine, Kyoto University Yoshida, Japan
    Science 265:1098-101. 1994
    ..Combined with the ability to genetically manipulate ES cells, this system will facilitate the study of molecular mechanisms involved in development and differentiation of hematopoietic cells...
  18. ncbi A functional c-myb gene is required for normal murine fetal hepatic hematopoiesis
    M L Mucenski
    Children s Hospital Research Foundation, Department of Pediatrics, Cincinnati, Ohio
    Cell 65:677-89. 1991
    ..By day 15, however, the mutant mice were severely anemic. Analysis indicated that embryonic erythropoiesis, which occurs in the yolk sac, was not impaired by the c-myb alteration...
  19. pmc Defective erythroid differentiation in miR-451 mutant mice mediated by 14-3-3zeta
    David M Patrick
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Genes Dev 24:1614-9. 2010
    ..We show that microRNA-451 (miR-451) regulates erythropoiesis in vivo...
  20. pmc Erythroblastic islands: niches for erythropoiesis
    Joel Anne Chasis
    Life Sciences Division, University of California, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Blood 112:470-8. 2008
    ..of erythroblast islands represent important contributors to normal erythroid development, as well as altered erythropoiesis found in such diverse diseases as anemia of inflammation and chronic disease, myelodysplasia, thalassemia, and ..
  21. pmc Severe malarial anemia: innate immunity and pathogenesis
    Douglas J Perkins
    Center for Global Health, Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque NM, USA
    Int J Biol Sci 7:1427-42. 2011
    ..destruction of parasitized and non-parasitized red blood cells (RBCs), inefficient and/or suppression of erythropoiesis, and dyserythropoiesis...
  22. ncbi Erythropoiesis: model systems, molecular regulators, and developmental programs
    Asterios S Tsiftsoglou
    Laboratory of Pharmacology, Department of Pharmaceutical Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece
    IUBMB Life 61:800-30. 2009
    Human erythropoiesis is a complex multistep developmental process that begins at the level of pluripotent hematopoietic stem cells (HSCs) at bone marrow microenvironment (HSCs niche) and terminates with the production of erythrocytes (..
  23. ncbi Development of erythroid and myeloid progenitors in the yolk sac and embryo proper of the mouse
    J Palis
    University of Rochester Medical Center, Department of Pediatrics and Cancer Center, Box 777, Rochester, NY 14642, USA rochester edu
    Development 126:5073-84. 1999
    ..The early wave of primitive erythropoiesis was followed by the appearance of definitive erythroid progenitors (BFU-E) that were first detectable at 1-7 ..
  24. pmc Regulation of erythropoiesis by hypoxia-inducible factors
    Volker H Haase
    Department of Medicine, Vanderbilt School of Medicine, Nashville, TN, USA
    Blood Rev 27:41-53. 2013
    ..Its key function in the hypoxic regulation of erythropoiesis is underscored by genetic studies in human populations that live at high-altitude and by mutational analysis ..
  25. pmc A global role for KLF1 in erythropoiesis revealed by ChIP-seq in primary erythroid cells
    Michael R Tallack
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia
    Genome Res 20:1052-63. 2010
    ..Additionally, we suggest new mechanisms for KLF1 cooperation with other transcription factors, in particular the erythroid transcription factor GATA1, to maintain homeostasis in the erythroid compartment...
  26. ncbi Novel erythropoiesis-stimulating agents: a new era in anemia management
    Iain C Macdougall
    Department of Renal Medicine, King s College Hospital, London, United Kingdom
    Clin J Am Soc Nephrol 3:200-7. 2008
    ..homology with erythropoietin, and yet is still able to activate the erythropoietin receptor and stimulate erythropoiesis. The search goes on for orally active antianemic therapies, and several strategies are being investigated, ..
  27. ncbi Involvement of oxygen-sensing pathways in physiologic and pathologic erythropoiesis
    Gregg L Semenza
    Vascular Program, Institute for Cell Engineering, McKusick Nathans Institute of Genetic Medicine, The Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, MD 21205, USA
    Blood 114:2015-9. 2009
    ..Intense efforts are under way to identify small molecule hydroxylase inhibitors that can be administered chronically to selectively induce erythropoiesis without undesirable side effects.
  28. ncbi MicroRNA expression profiling during human cord blood-derived CD34 cell erythropoiesis
    Meng Ling Choong
    Division of Biomedical Sciences, Singapore Immunology Network, Johns Hopkins in Singapore, 61 Biopolis Drive, Singapore 138673
    Exp Hematol 35:551-64. 2007
    MicroRNA (miRNA) expression profiling was performed on ex vivo differentiating erythroid cultures derived from human umbilical cord blood (UCB) CD34 cells and K562 cells to identify miRNAs involved in erythropoiesis.
  29. ncbi Generation of committed erythroid BFU-E and CFU-E progenitors does not require erythropoietin or the erythropoietin receptor
    H Wu
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Cell 83:59-67. 1995
    ..Both heterozygotes appeared normal. Homozygous animals exhibited reduced primitive erythropoiesis and died around embryonic day 13, owing to failure of definitive fetal liver erythropoiesis...
  30. pmc Resolving the distinct stages in erythroid differentiation based on dynamic changes in membrane protein expression during erythropoiesis
    Ke Chen
    Red Cell Physiology Laboratory, New York Blood Center, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:17413-8. 2009
    b>Erythropoiesis is the process by which nucleated erythroid progenitors proliferate and differentiate to generate, every second, millions of nonnucleated red cells with their unique discoid shape and membrane material properties...
  31. ncbi Effects of psychological stress on serum iron and erythropoiesis
    Chunlan Wei
    Department of Naval Medicine, Second Military Medical University, Shanghai 200433, China
    Int J Hematol 88:52-6. 2008
    ..However, so far whether pure psychological stress can cause decrease of serum iron and consequently affect erythropoiesis has not been reported...
  32. ncbi The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor
    Babs O Fabriek
    Department of Molecular Cell Biology, Vrije Universiteit Medical Center, Amsterdam, The Netherlands
    Blood 109:5223-9. 2007
    b>Erythropoiesis occurs in erythroblastic islands, where developing erythroblasts closely interact with macrophages. The adhesion molecules that govern macrophage-erythroblast contact have only been partially defined...
  33. ncbi Erythropoietin-mediated tissue protection: reducing collateral damage from the primary injury response
    M Brines
    Warren Pharmaceuticals, Ossining, NY 10562, USA
    J Intern Med 264:405-32. 2008
    ....
  34. pmc Foxo3 is required for the regulation of oxidative stress in erythropoiesis
    Dragan Marinkovic
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Clin Invest 117:2133-44. 2007
    ..In view of this striking finding, we investigated the potential role of Foxo3 in the regulation of ROS in erythropoiesis. Foxo3 expression, nuclear localization, and transcriptional activity were all enhanced during normal ..
  35. pmc The IRP1-HIF-2α axis coordinates iron and oxygen sensing with erythropoiesis and iron absorption
    Sheila A Anderson
    Department of Nutritional Sciences, University of Wisconsin Madison, Madison, WI 53706, USA
    Cell Metab 17:282-90. 2013
    ..These results uncover separable physiological roles of each IRP and identify IRP1 as a therapeutic target for manipulating HIF-2α action in hematologic, oncologic, and other disorders...
  36. pmc Essential role for Nix in autophagic maturation of erythroid cells
    Hector Sandoval
    Department of Immunology, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 454:232-5. 2008
    ..Our study may also provide insights into molecular mechanisms underlying mitochondrial quality control involving mitochondrial autophagy...
  37. pmc Combinatorial and distinct roles of α₅ and α₄ integrins in stress erythropoiesis in mice
    Tatiana Ulyanova
    Department of Medicine, Division of Hematology, University of Washington, Seattle, WA, USA
    Blood 117:975-85. 2011
    To delineate the role of specific members of β₁ integrins in stress erythropoiesis in the adult, we compared the response to phenylhydrazine stress in 3 genetically deficient models...
  38. ncbi The cloche and spadetail genes differentially affect hematopoiesis and vasculogenesis
    M A Thompson
    Howard Hughes Medical Institute, Children s Hospital, Boston, Massachusetts 02115, USA
    Dev Biol 197:248-69. 1998
    ..mutant embryos that carry a deletion of the c-myb gene demonstrates that c-myb is not required for primitive erythropoiesis in zebrafish even though it is expressed in these cells...
  39. pmc β-thalassemia: a model for elucidating the dynamic regulation of ineffective erythropoiesis and iron metabolism
    Yelena Ginzburg
    New York Blood Center, New York, NY 10065, USA
    Blood 118:4321-30. 2011
    thalassemia is a disease characterized by anemia and is associated with ineffective erythropoiesis and iron dysregulation resulting in iron overload...
  40. pmc Combinatorial assembly of developmental stage-specific enhancers controls gene expression programs during human erythropoiesis
    Jian Xu
    Division of Hematology Oncology, Children s Hospital Boston and Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 23:796-811. 2012
    ..Comparative analyses of human erythropoiesis identify developmental stage-specific enhancers as primary determinants of stage-specific gene expression ..
  41. ncbi microRNAs: fine tuning of erythropoiesis
    Marcin A Listowski
    Department of Molecular Biology, University of Zielona Gora, Poland
    Cell Mol Biol Lett 18:34-46. 2013
    ..attempts to summarize the state of knowledge on the role of miRNAs in the regulation of different stages of erythropoiesis. Original experimental data and results obtained with bioinformatics tools were combined to elucidate the ..
  42. pmc Networking erythropoiesis
    Marc A Kerenyi
    Division of Hematology Oncology, Children s Hospital Boston and the Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    J Exp Med 207:2537-41. 2010
    A relatively small cadre of lineage-restricted transcription factors largely orchestrates erythropoiesis, but how these nuclear factors interact to regulate this complex biology is still largely unknown...
  43. pmc MicroRNA-96 directly inhibits γ-globin expression in human erythropoiesis
    Imane Azzouzi
    Division of Haematology, University Children s Hospital, Zurich, Switzerland
    PLoS ONE 6:e22838. 2011
    ..The overexpression of microRNA-96 in human ex vivo erythropoiesis decreased γ-globin expression by 50%, whereas the knock-down of endogenous microRNA-96 increased γ-globin ..
  44. ncbi The expression of human blood group antigens during erythropoiesis in a cell culture system
    M J Southcott
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, UK
    Blood 93:4425-35. 1999
    ..Rh c antigen appeared before Rh D antigen, and it is suggested that this may reflect a reorganization of the developing erythroid cell membrane involving the Rh polypeptides and other components, including GPA and Band 3...
  45. pmc Heme oxygenase-1 deletion affects stress erythropoiesis
    Yu An Cao
    Department of Pediatrics, Stanford University School of Medicine, Stanford, California, United States of America
    PLoS ONE 6:e20634. 2011
    Homeostatic erythropoiesis leads to the formation of mature red blood cells under non-stress conditions, and the production of new erythrocytes occurs as the need arises...
  46. ncbi Expression and function of erythropoietin receptors in tumors: implications for the use of erythropoiesis-stimulating agents in cancer patients
    Angus M Sinclair
    Amgen Inc, Thousand Oaks, California, USA
    Cancer 110:477-88. 2007
    ..with previous clinical studies, which demonstrated no difference in survival for cancer patients who received erythropoiesis-stimulating agents (ESAs), some investigators have suggested a potential role for ESAs in promoting tumor ..
  47. pmc The HIF signaling pathway in osteoblasts directly modulates erythropoiesis through the production of EPO
    Erinn B Rankin
    Division of Radiation and Cancer Biology, Department of Radiation Oncology, Center for Clinical Sciences Research, Stanford University, Stanford, CA 94303 5152, USA
    Cell 149:63-74. 2012
    ..These data reveal an unexpected role for osteoblasts in the production of EPO and modulation of erythropoiesis. Furthermore, these studies demonstrate a molecular role for osteoblastic PHD/VHL/HIF signaling that can be ..
  48. pmc FOG-1-mediated recruitment of NuRD is required for cell lineage re-enforcement during haematopoiesis
    Zhiguang Gao
    Department of Medicine, The University of Chicago, Chicago, IL, USA
    EMBO J 29:457-68. 2010
    ..Homozygous FOG-1(R3K5A) mice were found to have splenomegaly, extramedullary erythropoiesis, granulocytosis and thrombocytopaenia secondary to a block in megakaryocyte maturation...
  49. pmc Immature cell populations and an erythropoiesis gene-expression signature in systemic juvenile idiopathic arthritis: implications for pathogenesis
    Claas H Hinze
    Division of Rheumatology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
    Arthritis Res Ther 12:R123. 2010
    ..observations suggest that active systemic juvenile idiopathic arthritis (sJIA) is associated with a prominent erythropoiesis gene-expression signature...
  50. pmc Hypoxic regulation of erythropoiesis and iron metabolism
    Volker H Haase
    Department of Medicine, Vanderbilt School of Medicine, Nashville, Tennessee 37232, USA
    Am J Physiol Renal Physiol 299:F1-13. 2010
    ..Efforts to understand the molecular basis of oxygen-regulated erythropoiesis have led to the identification of erythropoietin (EPO), which is essential for normal erythropoiesis and to ..
  51. pmc Suppression of Fas-FasL coexpression by erythropoietin mediates erythroblast expansion during the erythropoietic stress response in vivo
    Ying Liu
    Department of Pediatrics, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Blood 108:123-33. 2006
    ..Therefore, Fas and FasL are negative regulators of erythropoiesis. Epo-mediated suppression of erythroblast Fas and FasL is a novel stress response pathway that facilitates ..
  52. ncbi Primitive erythropoiesis in the mammalian embryo
    James Palis
    University of Rochester Medical Center, Department of Pediatrics, Center for Pediatric Biomedical Research, New York 14642, USA
    Int J Dev Biol 54:1011-8. 2010
    b>Erythropoiesis in adult mammals is characterized by the progressive maturation of hematopoietic stem cells to lineage-specific progenitors, to morphologically identifiable precursors which enucleate to form mature erythrocytes...
  53. ncbi KLF1 regulates BCL11A expression and gamma- to beta-globin gene switching
    Dewang Zhou
    Department of Biochemistry and Molecular Genetics and University of Alabama at Birmingham Stem Cell Institute, University of Alabama at Birmingham, Schools of Medicine and Dentistry, Birmingham, Alabama, USA
    Nat Genet 42:742-4. 2010
    ..Controlled knockdown of KLF1 in adult erythroid progenitors may provide a method to activate fetal hemoglobin expression in individuals with beta-thalassemia or sickle cell disease...
  54. pmc MicroRNAs 221 and 222 inhibit normal erythropoiesis and erythroleukemic cell growth via kit receptor down-modulation
    Nadia Felli
    Department of Hematology, Oncology, and Molecular Medicine, Istituto Superiore di Sanita, Rome, Italy
    Proc Natl Acad Sci U S A 102:18081-6. 2005
    ..Hypothetically, this decline could promote erythropoiesis by unblocking expression of key functional proteins...
  55. pmc Slc11a2 is required for intestinal iron absorption and erythropoiesis but dispensable in placenta and liver
    Hiromi Gunshin
    Children s Hospital Boston, Dana Farber Cancer Institute, Harvard Medical School, Massachusetts 02115, USA
    J Clin Invest 115:1258-66. 2005
    ..We previously showed that Slc11a2 serves as the primary portal for intestinal iron entry in hemochromatosis. However, inactivation of murine Hfe ameliorates the phenotype of animals lacking Slc11a2...
  56. pmc Control of globin gene expression during development and erythroid differentiation
    George Stamatoyannopoulos
    Department of Medicine, Division of Medical Genetics, University of Washington, Seattle, WA 98195, USA
    Exp Hematol 33:259-71. 2005
    ..Research in this field has an impact on understanding regulatory mechanisms in general and is of particular importance for eventual development of molecular cures for sickle cell disease and beta thalassemia...
  57. ncbi Pharmacokinetics of novel erythropoiesis stimulating protein compared with epoetin alfa in dialysis patients
    I C MacDougall
    Department of Renal Medicine, King s College Hospital, London, United Kingdom
    J Am Soc Nephrol 10:2392-5. 1999
    Novel erythropoiesis stimulating protein (NESP) is a hyperglycosylated analogue of recombinant human erythropoietin (Epoetin) which has an increased terminal half-life in animal models...
  58. ncbi Differential effects of an erythropoietin receptor gene disruption on primitive and definitive erythropoiesis
    C S Lin
    Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Genes Dev 10:154-64. 1996
    ..hormone erythropoietin (Epo) and its receptor (EpoR) are known to play important roles in the regulation of erythropoiesis, several questions remain concerning the developmental role of Epo/EpoR signaling...
  59. pmc Caspase activation is required for terminal erythroid differentiation
    Y Zermati
    Centre National de la Recherche Scientifique Unité Mixte de Recherche 8603, Universite Rene Descartes, Paris V, France
    J Exp Med 193:247-54. 2001
    ..These observations indicate a new function for caspases as key proteases in the process of erythroid differentiation...
  60. ncbi Novel insights into erythroid development revealed through in vitro differentiation of GATA-1 embryonic stem cells
    M J Weiss
    Division of Hematology Oncology, Children s Hospital, Dana Farber Cancer Institute, Boston, Massachusetts
    Genes Dev 8:1184-97. 1994
    ..interchangeability of GATA factors in vivo and suggest that GATA-1 normally serves to repress GATA-2 during erythropoiesis. The approach used here is a paradigm for the phenotypic analysis of targeted mutations affecting ..
  61. ncbi Inappropriately low reticulocytosis in severe malarial anemia correlates with suppression in the development of late erythroid precursors
    Kai Hsin Chang
    Institute of Parasitology, McGill University, Ste Anne de Bellevue, QC, Canada
    Blood 103:3727-35. 2004
    ..naive and infected mice were treated with recombinant murine erythropoietin (EPO), and the upstream events of erythropoiesis affected by blood-stage Plasmodium chabaudi AS were investigated...
  62. ncbi Inhibition of erythropoiesis in malaria anemia: role of hemozoin and hemozoin-generated 4-hydroxynonenal
    Oleksii A Skorokhod
    Department of Genetics, Biology, and Biochemistry, Laboratory of Cell Therapy, University of Torino Medical School, Torino, Italy
    Blood 116:4328-37. 2010
    Severe malaria anemia is characterized by inhibited/altered erythropoiesis and presence of hemozoin-(HZ)-laden bone-marrow macrophages...
  63. ncbi The MAPK ERK1 is a negative regulator of the adult steady-state splenic erythropoiesis
    Soizic Guihard
    Institut Cochin, Universite Paris Descartes, Centre National de la Recherche Scientifique CNRS Unité Mixte de Recherche UMR 8104, 75014 Paris, France
    Blood 115:3686-94. 2010
    ..Loss of ERK1 resulted in an enhanced splenic erythropoiesis, characterized by an accumulation of erythroid progenitors in the spleen, without any effect on the other ..
  64. ncbi Molecular insights into stress erythropoiesis
    Merav Socolovsky
    Department of Pediatrics, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Curr Opin Hematol 14:215-24. 2007
    In addition to its essential role in baseline erythropoiesis, the hormone erythropoietin drives the erythropoietic response to hypoxic stress...
  65. pmc Ineffective erythropoiesis with reduced red blood cell survival in serotonin-deficient mice
    Pascal Amireault
    Faculte de Medecine, Universite Paris Descartes, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8147, Hopital Necker, 75743 Paris Cedex 15, France
    Proc Natl Acad Sci U S A 108:13141-6. 2011
    ..data showing that mice deficient in peripheral 5-HT display morphological and cellular features of ineffective erythropoiesis. The central event occurs in the bone marrow where the absence of 5-HT hampers progression of erythroid ..
  66. pmc Maintenance of the BMP4-dependent stress erythropoiesis pathway in the murine spleen requires hedgehog signaling
    John M Perry
    Cell and Developmental Biology Option, Pennsylvania State University, University Park, PA 16802, USA
    Blood 113:911-8. 2009
    ..Here we examine the signals that are necessary for the maintenance of the BMP4-dependent stress erythropoiesis pathway...
  67. ncbi Multiple members of the TNF superfamily contribute to IFN-gamma-mediated inhibition of erythropoiesis
    Nadia Felli
    Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanita, Rome, Italy
    J Immunol 175:1464-72. 2005
    ....
  68. pmc Erythropoietin stimulates phosphorylation and activation of GATA-1 via the PI3-kinase/AKT signaling pathway
    Wei Zhao
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Blood 107:907-15. 2006
    ..These data demonstrate that EpoR signaling phosphorylates GATA-1 and modulates its activity via the PI3-kinase/AKT signaling pathway...
  69. ncbi BMP4 and Madh5 regulate the erythroid response to acute anemia
    Laurie E Lenox
    Department of Veterinary Science, Graduate Program in Biochemistry, Microbiology and Molecular Biology, and Huck Institute for Life Sciences Cell and Developmental Biology Option, Pennsylvania State University, University Park, PA 16802, USA
    Blood 105:2741-8. 2005
    ..The flexed-tail (f) mutant mice exhibit normal steady-state erythropoiesis but are unable to rapidly respond to acute erythropoietic stress...
  70. ncbi Inflammatory cytokine inhibition of erythropoiesis in patients implanted with a mechanical circulatory assist device
    Christopher N Pierce
    The University of Arizona Graduate Program in Circulatory Sciences, College of Medicine, Sarver Heart Center and University Medical Center, Tucson, AZ, USA
    Perfusion 20:83-90. 2005
    ..These results further support our previously published conclusion that a sufficient response of erythropoiesis occurs in reaction to the onset of anemia by an increased production of immature RBCs...
  71. ncbi An inherited mutation leading to production of only the short isoform of GATA-1 is associated with impaired erythropoiesis
    Luciana M Hollanda
    Department of Internal Medicine, Hemocentro, School of Medical Science, Universidade Estadual de Campinas, Campinas, Sao Paulo 13083 970, Brazil
    Nat Genet 38:807-12. 2006
    ..Experiments in mice suggest that GATA-1s supports normal adult megakaryopoiesis, platelet formation and erythropoiesis. Here we report a mutation, 332G --> C, in exon 2 of GATA1, leading to the synthesis of only the short isoform ..
  72. ncbi Erythropoiesis-stimulating agent hyporesponsiveness
    David W Johnson
    Department of Renal Medicine, University of Queensland at Princess Alexandra Hospital, Brisbane, Queensland, Australia
    Nephrology (Carlton) 12:321-30. 2007
    Approximately 5-10% of patients with chronic kidney disease demonstrate hyporesponsiveness to erythropoiesis-stimulating agents (ESA), defined as a continued need for greater than 300 IU/kg per week erythropoietin or 1...
  73. pmc Increased erythropoiesis in mice injected with submicrogram quantities of pseudouridine-containing mRNA encoding erythropoietin
    Katalin Kariko
    Department of Neurosurgery, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Mol Ther 20:948-53. 2012
    ..These results demonstrate that HPLC-purified, pseudouridine-containing mRNAs encoding therapeutic proteins have great potential for clinical applications...
  74. pmc TAM receptors and the regulation of erythropoiesis in mice
    Hongmei Tang
    Department of Cell Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, P R China
    Haematologica 94:326-34. 2009
    TAM receptors (Tyro3, Axl and Mer) are expressed in hematopoietic tissues. The roles of the three receptors in hematopoiesis are, however, largely unknown. We investigated the role of TAM receptors in regulating erythropoiesis.
  75. pmc Anemia, ineffective erythropoiesis, and hepcidin: interacting factors in abnormal iron metabolism leading to iron overload in β-thalassemia
    Sara Gardenghi
    Hematology Oncology, Department of Pediatrics, Weill Cornell Medical College, 515 East 71st Street, New York, NY 10021, USA
    Hematol Oncol Clin North Am 24:1089-107. 2010
    ..disorder caused by mutations in the β-globin gene and characterized by chronic anemia caused by ineffective erythropoiesis, and accompanied by a variety of serious secondary complications such as extramedullary hematopoiesis, ..
  76. pmc A KRAB/KAP1-miRNA cascade regulates erythropoiesis through stage-specific control of mitophagy
    Isabelle Barde
    School of Life Sciences and Frontiers in Genetics Program, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Science 340:350-3. 2013
    ..The KRAB/KAP1-miRNA regulatory cascade is evolutionarily conserved, as it also controls mitophagy during human erythropoiesis. Thus, a multilayered transcription regulatory system is present, in which protein- and RNA-based repressors ..
  77. pmc Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis
    Anupama Narla
    Department of Medicine, Brigham and Women s Hospital, Boston, MA, USA
    Blood 118:2296-304. 2011
    ..Both agents promote erythropoiesis, increasing the absolute number of erythroid cells produced from normal CD34(+) cells and from CD34(+) cells ..
  78. ncbi Chronic IFN-γ production in mice induces anemia by reducing erythrocyte life span and inhibiting erythropoiesis through an IRF-1/PU.1 axis
    Sten F Libregts
    Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Blood 118:2578-88. 2011
    ..1 (PU.1) and induces PU.1 expression, leading to inhibition of erythropoiesis. Notably, down-regulation of either IRF-1 or PU...
  79. pmc Proof of principle for transfusion of in vitro-generated red blood cells
    Marie Catherine Giarratana
    UPMC Paris, UMR_S938 CDR Saint Antoine, Prolifération et Différenciation des Cellules Souches, France
    Blood 118:5071-9. 2011
    ..These data establish the proof of principle for transfusion of in vitro-generated RBCs and path the way toward new developments in transfusion medicine. This study is registered at http://www.clinicaltrials.gov as NCT0929266...
  80. pmc ETO-2 associates with SCL in erythroid cells and megakaryocytes and provides repressor functions in erythropoiesis
    Anna H Schuh
    MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
    Mol Cell Biol 25:10235-50. 2005
    ....
  81. ncbi Expression analyses and transcriptional regulation of mouse nucleolar spindle-associated protein gene in erythroid cells: essential role of NF-Y
    Tohru Fujiwara
    Department of Rheumatology and Haematology, Tohoku University Graduate School of Medicine, Sendai, Japan
    Br J Haematol 135:583-90. 2006
    ..To characterise its role in erythropoiesis, we examined the expression profile of the NuSAP gene...
  82. pmc During EPO or anemia challenge, erythroid progenitor cells transit through a selectively expandable proerythroblast pool
    Arvind Dev
    COBRE Center of Excellence in Stem Cell Biology and Regenerative Medicine, Maine Medical Center Research Institute, Scarborough, ME, USA
    Blood 116:5334-46. 2010
    ..Overall, findings reveal a novel transitional proerythroblast compartment that deploys unique expansion devices...
  83. ncbi Yolk-sac hematopoiesis: the first blood cells of mouse and man
    J Palis
    University of Rochester, Department of Pediatrics and Cancer Center, Rochester, NY 14642, USA
    Exp Hematol 29:927-36. 2001
    ..To review the process of blood-cell formation in the murine and human yolk sac...
  84. ncbi Malarial anaemia: mechanisms and implications of insufficient erythropoiesis during blood-stage malaria
    Kai Hsin Chang
    Centre for Host Parasite Interactions, Institute of Parasitology, McGill University, Ste Anne de Bellevue, Que, Canada
    Int J Parasitol 34:1501-16. 2004
    ..Inappropriately low reticulocytosis is observed in malaria patients suggesting that insufficient erythropoiesis is a major factor...
  85. ncbi Iron and erythropoiesis: a dual relationship
    Clara Camaschella
    Division of Genetics and Cell Biology, Università Vita Salute e IRCCS San Raffaele, Via Olgettina 60, 20132 Milan, Italy
    Int J Hematol 93:21-6. 2011
    Iron is essential for cell life and especially for erythropoiesis which is the major body consumer of iron for red cell production...
  86. pmc Early mammalian erythropoiesis requires the Dot1L methyltransferase
    Yi Feng
    Department of Pathology and Laboratory Medicine, Division of Cancer and Developmental Biology, Institute for Reproductive Health and Regenerative Medicine, University of Kansas Medical Center, Kansas City, KS, USA
    Blood 116:4483-91. 2010
    ..GATA2, a factor essential for early erythropoiesis, was significantly reduced in Dot1L-deficient cells, whereas expression of PU...
  87. ncbi Design and chemical synthesis of a homogeneous polymer-modified erythropoiesis protein
    Gerd G Kochendoerfer
    Gryphon Therapeutics, 250 East Grand Avenue, Suite 90, South San Francisco, CA 94080, USA
    Science 299:884-7. 2003
    We report the design and total chemical synthesis of "synthetic erythropoiesis protein" (SEP), a 51-kilodalton protein-polymer construct consisting of a 166-amino-acid polypeptide chain and two covalently attached, branched, and ..
  88. pmc Innate immune activation during Salmonella infection initiates extramedullary erythropoiesis and splenomegaly
    Amy Jackson
    Center for Infectious Diseases and Microbiology Translational Research, Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    J Immunol 185:6198-204. 2010
    ..infection, the majority of spleen cells are immature CD71(-)Ter119(+) reticulocytes, indicating that massive erythropoiesis occurs in response to Salmonella infection...
  89. ncbi Salidroside promotes erythropoiesis and protects erythroblasts against oxidative stress by up-regulating glutathione peroxidase and thioredoxin
    Erin Wei Qian
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong
    J Ethnopharmacol 133:308-14. 2011
    ..Rhodiola rosea is commonly used in China and Tibet folk medicine for the treatment of high altitude sickness, anoxia and mountain malhypoxia...
  90. ncbi Erythropoietin receptor haploinsufficiency and in vivo interplay with granulocyte-macrophage colony-stimulating factor and interleukin 3
    Armin G Jegalian
    Department of Molecular and Medical Pharmacology, Molecular Biology Institute, University of California Los Angeles School of Medicine, Los Angeles, CA 90095 1735, USA
    Blood 99:2603-5. 2002
    Erythropoietin (EPO) and its receptor (EPOR) are critical for definitive erythropoiesis, as mice lacking either gene product die during embryogenesis with severe anemia...
  91. pmc Progression through key stages of haemopoiesis is dependent on distinct threshold levels of c-Myb
    Nikla Emambokus
    Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, UK
    EMBO J 22:4478-88. 2003
    ..of macrophage and megakaryocytes, while higher levels seem to be particularly important in the control of erythropoiesis and lymphopoiesis...
  92. pmc Gene induction and repression during terminal erythropoiesis are mediated by distinct epigenetic changes
    Piu Wong
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Blood 118:e128-38. 2011
    It is unclear how epigenetic changes regulate the induction of erythroid-specific genes during terminal erythropoiesis. Here we use global mRNA sequencing (mRNA-seq) and chromatin immunoprecipitation coupled to high-throughput sequencing (..
  93. pmc ABCB6 is dispensable for erythropoiesis and specifies the new blood group system Langereis
    Virginie Helias
    National Institute of Blood Transfusion INTS, Paris, France
    Nat Genet 44:170-3. 2012
    ..Of note, Lan(-) (ABCB6(-/-)) individuals do not suffer any clinical consequences, although their deficiency in ABCB6 may place them at risk when determining drug dosage...
  94. pmc Regulation of erythroid differentiation by miR-376a and its targets
    Fang Wang
    Department of Biochemistry and Molecular Biology, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China
    Cell Res 21:1196-209. 2011
    ..Here we report that miR-376a participates in the regulation of the early stages of human erythropoiesis by targeting cyclin-dependent kinase 2 (CDK2) and Argonaute 2 (Ago2)...
  95. ncbi Polymeric IgA1 controls erythroblast proliferation and accelerates erythropoiesis recovery in anemia
    Séverine Coulon
    Centre National de la Recherche Scientifique CNRS Unité Mixte Recherche UMR 8147, Universite Paris Descartes, Faculte de Medecine, Hopital Necker, Paris, France
    Nat Med 17:1456-65. 2011
    ..Our results show that pIgA1 and TfR1 are positive regulators of erythropoiesis in both physiological and pathological situations...
  96. pmc Genome architecture of the human beta-globin locus affects developmental regulation of gene expression
    Susanna Harju
    Department of Biochemistry and Molecular Biology, MS 3030, University of Kansas Medical Center, Kansas City, 66160 7421, USA
    Mol Cell Biol 25:8765-78. 2005
    ..control region (LCR) and the epsilon-globin gene, beta(m)-globin expression dominated primitive and definitive erythropoiesis; only beta(m)-globin mRNA was detected during the fetal and adult definitive stages of erythropoiesis...
  97. ncbi Fetal and adult hemoglobin production during adult erythropoiesis: coordinate expression correlates with cell proliferation
    Urszula Wojda
    Laboratory of Chemical Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 99:3005-13. 2002
    ..sickle cell and beta-thalassemia syndromes rely on our understanding of hemoglobin accumulation during human erythropoiesis. Here we report direct measurements of hemoglobin composition and messenger RNA (mRNA) levels in cultured CD34(..
  98. ncbi Novel strategies for stimulating erythropoiesis and potential new treatments for anaemia
    Iain C Macdougall
    Department of Renal Medicine, King s College Hospital, London SE5 9RS, UK
    Lancet 368:947-53. 2006
    ..and gene transfer) have resulted in the development of potential new agents and strategies for stimulating erythropoiesis. These advances are of possible benefit in treating anaemia due to various causes, including chronic renal ..
  99. ncbi Treating anemia in heart failure patients: a review of erythropoiesis-stimulating agents
    Benjamin P Geisler
    Massachusetts General Hospital, Institute for Technology Assessment, Harvard Medical School, Boston, MA, USA
    Expert Opin Biol Ther 10:1209-16. 2010
    ..Prevalence of chronic heart failure (CHF) is increasing, and despite improvements in the past decade the prognosis in terms of mortality and health-related quality of life remains poor. Anemia is often found concomitantly in CHF patients...
  100. ncbi Roles of hematopoietic transcription factors GATA-1 and GATA-2 in the development of red blood cell lineage
    Kinuko Ohneda
    Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan
    Acta Haematol 108:237-45. 2002
    The transcription factors GATA-1 and GATA-2 play key roles in gene regulation during erythropoiesis. Gene ablation studies in mouse revealed that GATA-2 is crucial for the maintenance and proliferation of immature hematopoietic ..
  101. ncbi Targeted deletion of Nm23/nucleoside diphosphate kinase A and B reveals their requirement for definitive erythropoiesis in the mouse embryo
    Edith H Postel
    Laboratory of Biochemistry and Molecular Biology, Department of Pediatrics, Robert Wood Johnson Medical School UMDNJ and Cancer Institute of New Jersey, Medical Education Building, New Brunswick, New Jersey 08903 0019, USA
    Dev Dyn 238:775-87. 2009
    ..We conclude that Nm23/NDPKs play critical roles in definitive erythroid development. Our novel mouse model also links erythropoiesis and nucleotide metabolism.

Research Grants69

  1. Mechanisms of hepcidin effects in the anemia of chronic disease
    Robert T Means; Fiscal Year: 2013
    ..The marrow cannot respond adequately to this demand due to impaired erythropoiesis and impaired mobilization of reticuloendothelial system iron stores...
  2. Role of the Mammalian Siderophore in Cellular Iron Metabolism and Erythropoiesis
    Laxminarayana R Devireddy; Fiscal Year: 2012
    ..If successful, this grant could help explain the causes of iron deficiency anemia and could help in designing better drugs to combat iron deficiency anemia. ..
  3. Impacts of surface receptors [Toll like receptor (TLRs) and Fc gamma receptors (F
    Collins Ouma; Fiscal Year: 2012
    ..direct destruction of red blood cells (RBCs) by parasites, hemolysis of non-parasitized RBCs, suppression of erythropoiesis, erythrophagocytosis, and dysregulation in cytokine production...
  4. Molecular Biology of Human Erythrocyte Alpha Spectrin
    Patrick G Gallagher; Fiscal Year: 2013
    ..Results obtained from these studies will allow study of the role of 1-spectrin in erythropoiesis, membrane biogenesis, and inherited erythrocyte disorders...
  5. Mechanistic Characterization and Genetic Analysis of Systemic Iron Homeostasis
    Xin Du; Fiscal Year: 2011
    ..of heme and a constituent of many iron-dependent enzymes, iron is essential for oxidative phosphorylation, erythropoiesis, and many other chemical reactions that are vital for survival of almost all living organisms...
  6. Biological Functions of Iron Responsive Elements
    Richard S Eisenstein; Fiscal Year: 2012
    ..IRP1-/- mice have profound disturbances in erythropoiesis, including a transient severe polycythemia, and display other symptoms observed in humans with HIF2[unreadable]..
  7. TSAD AS AN EPOR/JAK2 ADAPTOR AND ERYTHROPOIETIC REGULATOR
    DONALD JOSEPH MCCRANN; Fiscal Year: 2010
    ..is to define roles of TSAD (T-cell specific adaptor protein) in a novel context of EPOR/JAK2 dependent erythropoiesis. This NRSA fellowship opportunity will foster advanced skills and knowledge in erythropoiesis, and will ..
  8. Modifiers of hepcidin expression as new therapies for iron overload
    PAULA GOODMAN FRAENKEL; Fiscal Year: 2013
    ..will be evaluated in mouse models of thalassemia and hemochromatosis for their effects on iron overload and erythropoiesis. These studies will lead to the identification of molecules and pathways that may be adapted to generate ..
  9. VEGF Regulation of Hepatic Erythropoietin Synthesis
    Calvin Kuo; Fiscal Year: 2009
    ..Either insufficient or excess erythropoiesis produces disease in humans, with too few RBCs resulting in anemia, and too many RBCs resulting in polycythemia ..
  10. Understand and Improve Iron Distribution and Erythropoiesis in Beta-Thalassemia
    Yelena Ginzburg; Fiscal Year: 2013
    ..Beta-thalassemia in humans is a disease associated with anemia, splenomegaly, and ineffective erythropoiesis;iron overload in this disease results from transfusion and increased iron absorption from hepcidin deficiency...
  11. SIGNAL TRANSDUCTION MECHANISMS OF ERYTHROPOIETIN
    Barbara A Miller; Fiscal Year: 2011
    ..human erythroid progenitors/precursors to study mechanisms of regulation of TRPC3 and their role in human erythropoiesis. Specific Aim 2 will examine the mechanisms through which TRPC6 inhibits TRPC3 activation by Epo and the ..
  12. AKT Signaling and Oxidative Stress Regulation of Erythropoiesis
    Saghi Ghaffari; Fiscal Year: 2013
    ..for daily production of erythroid cells and for immediate response to clinical conditions in which stress erythropoiesis is in demand...
  13. Biological Functions of Iron Responsive Elements
    Richard S Eisenstein; Fiscal Year: 2013
    ..IRP1-/- mice have profound disturbances in erythropoiesis, including a transient severe polycythemia, and display symptoms of dysregulated stress erythropoiesis...
  14. Novel Erythroid Cell Membrane Protein
    Emery H Bresnick; Fiscal Year: 2010
    ..There is great interest in developing new strategies to modulate erythropoiesis, and in this regard, we have been analyzing GATA transcription factor function...
  15. Effects of Vitamin D and Marine Omega-3 Fatty Acids on Anemia in the Elderly
    Nancy Berliner; Fiscal Year: 2013
    ..using a sub-sample of the VITAL cohort, we will investigate whether vitamin D and/or ?-3 FA influence erythropoiesis via hepcidin- and/or erythropoietin (EPO)-dependent pathways...
  16. Functional Genomic Dissection of Refractory Anemia
    BENJAMIN LEVINE EBERT; Fiscal Year: 2013
    ..The finding that RPS14 haploinsufficiency causes a block in erythropoiesis, the dominant phenotype of the 5q- syndrome, established a previously unrecognized link between the 5q- ..
  17. Nonsense Mediated RNA Decay Regulation in Erythropoiesis &Thalassemia
    LAWRENCE BENJAMIN GARDNER; Fiscal Year: 2012
    ..and genetic studies have demonstrated that the regulation of this phosphorylation plays an important role in erythropoiesis. We have determined that the inhibition of nonsense mediated RNA decay in hypoxic cells is dependent on eIF2a ..
  18. A Comparative Approach to Globin Regulation/Thalassemia
    LEONARD IRA ZON; Fiscal Year: 2012
    ..specific genes are injected into the one cell embryo can knock-down gene function and will reveal changes in erythropoiesis. A compendium of over 300 chromatin remodeling factors that are orthologs of human genes has been created, and ..
  19. The p110 alpha and delta isoforms of PI3 kinase in hematopoiesis and leukemia
    Kira Gritsman; Fiscal Year: 2013
    ..Preliminary data reveal that conditional deletion of p110 in HSCs results in impaired erythropoiesis, and slightly reduced HSC repopulating ability...
  20. Novel Immunodeficiency caused by TFRC Mutation
    RAIF SALIM GEHA; Fiscal Year: 2013
    ..Surprisingly, despite the critical role of TfR1-mediated iron transport in erythropoiesis, the patients had little or no microcytic anemia...
  21. MODELING AND RESCUE OF DBA IN THE MOUSE
    Philip J Mason; Fiscal Year: 2010
    ..However, the relationship between ribosomal protein deficiency and defective erythropoiesis is not known...
  22. Defining Estrogenic Regulation of Hematopoietic Stem Cell Formation and Function
    Trista E North; Fiscal Year: 2013
    ..impact HSC formation during multiple distinct periods during development, affecting the primitive wave of erythropoiesis, the formation of the vascular hematopoietic niche and HSC proliferation...
  23. Characterization of a Novel Erythropoietin Signaling Pathway
    Adam N Goldfarb; Fiscal Year: 2013
    ..provided by applicant): The soluble cytokine erythropoietin (Epo) plays a central role in physiologic erythropoiesis and is a mainstay in treatment of human anemias...
  24. Control of the Erythroid Terminal Differentiation Decision
    Arthur I Skoultchi; Fiscal Year: 2013
    ..We also propose to investigate roles for CDK6 and E2F2 in stress erythropoiesis and in ex vivo self-renewal of erythroid precursors...
  25. Long-acting EPO analog for treating anemia associated with chronic kidney disease
    GEORGE NORBERT COX; Fiscal Year: 2010
    ..EPO analog that has a significantly increased circulating half-life and superior efficacy in animal erythropoiesis models compared to EPO...
  26. Role of Protective and Pathogenic B cells in Modulating Pediatric Severe Malaria
    Tom Were; Fiscal Year: 2013
    ..has been attributed to direct and indirect destruction of infected and uninfected erythrocytes, suppression of erythropoiesis, erythrophagocytosis, and dysregulation in cytokine production...
  27. Enhancement of Medullary Erythropoiesis during Anemia by Trib3
    David Kuhrt; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Anemia can induce frequent bouts of stress erythropoiesis as accelerated red blood cell production. In chronic disease settings, these bouts often become compromised...
  28. GATA1 Mutation in Defective Erythropoiesis
    John D Crispino; Fiscal Year: 2013
    ..The research described in this proposal will greatly expand our insights into how loss of the N-terminus of GATA1 alters erythropoiesis and will benefit patients with congenital anemia and DS-AMKL.
  29. Cell Therapies for Cooley's Anemia
    Thomas M Ryan; Fiscal Year: 2013
    ..study of the regulation of globin gene expression, synthesis, and switching;studies of stress and ineffective erythropoiesis;development of transfusion and iron chelation therapies;studies of iron homeostasis in disease;induction of ..
  30. The role of erythroferrone in iron homeostasis and in iron-loading anemias
    Tomas Ganz; Fiscal Year: 2013
    ..the marrow comes under particular strain after hemorrhage, hemolysis, and other events that trigger expanded erythropoiesis. Both the absorption of dietary iron and the release of iron from stores are increased as erythropoiesis ..
  31. Mechanisms of Oxygen Sensing
    Navdeep S Chandel; Fiscal Year: 2013
    ..genes resulting in activation of a genetic program that includes upregulation of glycolysis, angiogenesis, and erythropoiesis. Under normoxic conditions, the HIF[unreadable] protein subunit is hydroxylated by Prolyl Hydroxylase Domain ..
  32. DEVELOPMENTAL BIOLOGY OF HUMAN ERYTHROPOIESIS
    Stuart H Orkin; Fiscal Year: 2013
    ..abstract_text> ..
  33. Cytoskeletal Pliability within Cells under Stress: RBCs and Marrow-derived SCs
    Dennis E Discher; Fiscal Year: 2012
    ..Since red cell membrane diseases and disordered erythropoiesis feature in a number of clinically relevant diseases our findings will likely have significant clinical ..
  34. Mechanisms of HbF Activation by Non-deletional HPFH
    Kenneth R Peterson; Fiscal Year: 2013
    ..Thus, knowledge of how to reactivate ?-globin (HbF) in adult erythropoiesis will benefit SCD patients...
  35. Hepcidin therapy for iron overload and hematologic disorders
    Stefano Rivella; Fiscal Year: 2013
    ..effects in hemochromatosis and ?-thalassemia, and examine the effects of hepcidin agonists on ?-thalassemic erythropoiesis in mouse models and in bone marrow cultures and identify those mechanisms that could ameliorate anemia...
  36. Rac1 and Rac2 Guanosine Triphosphatases in Erythroid Function and Differentiation
    THEODOSIA ANASTASIOS KALFA; Fiscal Year: 2012
    ..b>Erythropoiesis is affected mainly at the early stages, when stem cell factor (SCF) plays a critical role...
  37. Hematological consequences of FLVCR, a cytoplasmic heme export protein
    Janis L Abkowitz; Fiscal Year: 2013
    ..synthesis precedes or exceeds globin synthesis and that if these steps were poorly coordinated, ineffective erythropoiesis and anemia would result...
  38. Barrier Insulators in Erythropoiesis
    Patrick G Gallagher; Fiscal Year: 2013
    ..Project Summary - Abstract Barrier Insulators in Erythropoiesis Barrier insulators create a "barrier" to protect against heterochromatin-mediated gene silencing, critical for ..
  39. The role of Dock4 in normal and aberrant erythropoiesis
    Amittha Wickrema; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Erythropoiesis is tightly controlled at every phase of differentiation...
  40. Multi-level analysis of iron metabolism and treatment of chronic kidney disease
    Paul L Fox; Fiscal Year: 2013
    ..clinicians with a tool for personalized treatment of CKD (and potentially other anemias) that can optimize erythropoiesis, minimize cost, and most importantly, reduce adverse side-effects such as cardiac hypertrophy and failure...
  41. Opposing and complementary roles of KLF1 and KLF2 in erythropoiesis
    Joyce A Lloyd; Fiscal Year: 2012
    ..and complementary roles of Kr[unreadable]ppel-like factor 1 (EKLF or KLF1) and KLF2 in embryonic and fetal erythropoiesis. This is biologically important because it could facilitate effective therapeutic strategies for the ..
  42. Pathways of Maternal Anemia
    Andrew V Oleinikov; Fiscal Year: 2013
    ..We hypothesize that proinflammatory signaling pathways in primigravid women modify the expression of erythropoiesis-specific factors in the placenta, leading to anemia through one or both of the following pathways. 1...
  43. HRI-eIF2a Phosphorylation Signaling in Oxidative Stress and Erythropoiesis
    Jane Jane Chen; Fiscal Year: 2012
    ..is to contribute to the more comprehensive understanding of the regulation of hemoglobin synthesis and erythropoiesis under stress conditions and in red cell disorders...
  44. BMP inhibitors and the study of disease mechanisms in anemia of inflammation
    Randall T Peterson; Fiscal Year: 2012
    ..Increased hepcidin expression decreases serum levels of iron, reducing its availability for erythropoiesis. Recent evidence has suggested a pivotal role for bone morphogenetic proteins (BMPs) in regulating hepcidin ..
  45. Acute vs. delayed iron therapy: effect on iron status, anemia and cognition
    Chandy C John; Fiscal Year: 2013
    ..Hemoglobin, iron status (ZnPP, ferritin, TfR), erythropoiesis (erythropoietin, reticulocyte count) and inflammation (hepcidin, ferritin, alphal-acid glycoprotein, cytokines)..
  46. Ubiquitination in erythropoiesis and the pathophysiology of anemia
    Mark D Fleming; Fiscal Year: 2013
    ....
  47. Brain imaging and developmental follow-up of infants treated with erythropoietin
    Robin K Ohls; Fiscal Year: 2013
    ..One promising intervention is the use of recombinant erythropoietin (Epo, also known as an erythropoiesis stimulating agent, or ESA)...
  48. Preterm Epo Neuroprotection Trial (PENUT Trial) CCC
    Sandra E Juul; Fiscal Year: 2013
    ..It is widely available, affordable, and has been used safely in neonates to stimulate erythropoiesis. There are extensive preclinical data to support its use as a neuroprotective intervention: Epo decreases ..
  49. Study of bone disease in Thalassemia
    Maria G Vogiatzi; Fiscal Year: 2010
    ..It is hypothesized that the bone loss is thalassemia is associated with ineffective erythropoiesis, improper iron handling and iron chelation, which result in impaired bone formation...
  50. MicroRNAs and hematopoietic differentiation
    Harvey F Lodish; Fiscal Year: 2013
    ..down miR-15a-16-1 expression in purified cultured erythroid progenitors and by analyzing fetal and adult erythropoiesis in mice deleted for the miR15a-16-1 locus...
  51. Novel Determinants of Terminal Erythroid Maturation
    Saghi Ghaffari; Fiscal Year: 2013
    ..signaling, which would constitute a new paradigm with considerable potential for therapeutic modulation of erythropoiesis and understanding erythroid pathophysiologies...
  52. Role of the STK Receptor in Erythropoiesis
    Pamela Hankey; Fiscal Year: 2009
    ....
  53. In vivo function of macrophage in healthy and diseased erythropoiesis
    Paul S Frenette; Fiscal Year: 2013
    ..using in vitro reconstitution analyses have clearly suggested a role for marrow-derived M[unreadable] in erythropoiesis, there is currently no in vivo evidence that M[unreadable] are required or even play a role in postnatal ..
  54. Activation and Silencing of Gene Expression during Hematopoiesis--OLD
    Mark T Groudine; Fiscal Year: 2013
    ..locus, and tethering of wildtype (WT) and mutant loci to nuclear pore complexes (NPC) and lamina during murine erythropoiesis. We will also determine the genome-wide alterations in peripheral localization and expression state during ..
  55. BMP4 dependent stress erythropoiesis pathway in short-term radioprotection
    ROBERT FRANK PAULSON; Fiscal Year: 2013
    ..This process is referred to as steady state erythropoiesis. In response to acute anemic challenge, the situation is different...
  56. Effects of developmental stage and of KLF2 on mouse erythroid genetic profiles
    Joyce A Lloyd; Fiscal Year: 2011
    ..In our Preliminary Studies and the work of others, candidate regulators of primitive erythropoiesis include Rnd2, Slit 2, reelin and LMO2, and members of the BMP, VEGF and retinoid signaling pathways...
  57. Preterm Epo Neuroprotection Trial (PENUT Trial) DCC
    Patrick J Heagerty; Fiscal Year: 2013
    ..It is widely available, affordable, and has been used safely in neonates to stimulate erythropoiesis. There are extensive preclinical data to support its use as a neuroprotective intervention: Epo decreases ..
  58. 5-Hydroxymethylcytosine and Globin Gene Switching
    Donald Lavelle; Fiscal Year: 2013
    ..showing that the gamma-globin gene promoter was demethylated in a progressive manner during fetal liver erythropoiesis. The mechanism responsible for DNA demethylation of the gamma-globin promoter during fetal liver ..
  59. Function of human &mouse Beta-globin locus control regions
    Mark T Groudine; Fiscal Year: 2013
    ..basis for initiating and propagating the chromatin and transcription states of the ?-globin locus during erythropoiesis. Our Specific Aims include: 1...
  60. Elucidation of Novel Anti-apoptotic Pathways in Erythropoiesis
    Yongping Wang; Fiscal Year: 2013
    ..Wilson, an internationally renowned geneticist, and Dr. Mitchell Weiss, an international authority on erythropoiesis. The PI will be advised by a committee of highly regarded scientists, including Drs...
  61. Mechanisms of Impaired Erythropoiesis in Post Burn Anemia of Critical Illness
    KUZHALI MUTHUMALAIAPPAN; Fiscal Year: 2013
    ..Taken together, our overarching hypothesis states that post burn anemia of critical illness is due to impaired erythropoiesis, which is orchestrated by a combination of dysregulated hematopoietic transcription factors and burn ..
  62. HRI-eIF2a Phosphorylation Signaling in Oxidative Stress and Erythropoiesis
    Jane Jane Chen; Fiscal Year: 2013
    ..is to contribute to the more comprehensive understanding of the regulation of hemoglobin synthesis and erythropoiesis under stress conditions and in red cell disorders...
  63. ERYTHROPOIESIS AND HEMOGLOBIN REGULATION IN MAN
    BLANCHE ALTER; Fiscal Year: 1990
    b>Erythropoiesis and hemoglobin regulation will be examined in vitro and in vivo...
  64. Model to study the effects of ethanol on erythropoiesis
    Stephen Koury; Fiscal Year: 2001
    ..abstract_text> ..
  65. ERYTHROPOIETIN AND THE REGULATION OF ERYTHROPOIESIS
    Sanford Krantz; Fiscal Year: 2003
    The aim of this research is to enhance understanding of the regulation of erythropoiesis and apply this knowledge to clinical anemias...
  66. Feline Leukemia Virus C Receptor Heme Export and Erythroid Differentiation
    SIOBAN BRIDGET KEEL; Fiscal Year: 2010
    ..virus, subgroup C (FeLV-C) develop profound pure red cell aplasia (PRCA) or on rare occasion ineffective erythropoiesis. Our lab previously demonstrated that the cell surface receptor for feline leukemia virus, subgroup C (FLVCR) ..
  67. ERYTHROPOIETIN THERAPY IN RENAL FAILURE
    JOSEPH ESCHBACH; Fiscal Year: 1991
    Our purpose is to investigate the reasons why erythropoiesis remains suppressed in the majority of maintenance dialysis patients...
  68. Molecular Biology of Polycythemia
    Josef Prchal; Fiscal Year: 2007
    The overall objective of this proposal is to examine the mechanisms of control of erythropoiesis concentrating on those that, when disturbed, result in polycythemia...
  69. RADIOASSAY OF ERYTHROPOIETIN
    GISELA CLEMONS; Fiscal Year: 1991
    ..normal circulating levels, but also depressed levels seen following physiological conditions known to depress erythropoiesis, and increased hormone levels associated with stimuli known to increase erythropoiesis, such as bleeding, ..