cell polarity


Summary: Orientation of intracellular structures especially with respect to the apical and basolateral domains of the plasma membrane. Polarized cells must direct proteins from the Golgi apparatus to the appropriate domain since tight junctions prevent proteins from diffusing between the two domains.

Top Publications

  1. ncbi Cell migration: integrating signals from front to back
    Anne J Ridley
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London W1W 7BS, UK
    Science 302:1704-9. 2003
  2. ncbi Rho GTPases in cell biology
    Sandrine Etienne-Manneville
    MRC Laboratory for Molecular Cell Biology and Cell Biology Unit, Cancer Research UK Oncogene and Signal Transduction Group, University College London, Gower Street, London WC1E 6BT, UK
    Nature 420:629-35. 2002
  3. ncbi The cell biology of neurogenesis
    Magdalena Gotz
    Institute for Stem Cell Research, GSF National Research Center for Environment and Health, Ingolstadter Landstrasse 1, D 85764 Neuherberg Munich, Germany
    Nat Rev Mol Cell Biol 6:777-88. 2005
  4. ncbi Eukaryotic chemotaxis: a network of signaling pathways controls motility, directional sensing, and polarity
    Kristen F Swaney
    Department of Cell Biology, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA
    Annu Rev Biophys 39:265-89. 2010
  5. ncbi Epithelial cell polarity, stem cells and cancer
    Fernando Martin-Belmonte
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Madrid 28049, Spain
    Nat Rev Cancer 12:23-38. 2012
  6. ncbi Tracking the ends: a dynamic protein network controls the fate of microtubule tips
    Anna Akhmanova
    Department of Cell Biology, Erasmus Medical Center, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Nat Rev Mol Cell Biol 9:309-22. 2008
  7. pmc A sterol-regulatory element binding protein is required for cell polarity, hypoxia adaptation, azole drug resistance, and virulence in Aspergillus fumigatus
    Sven D Willger
    Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT, USA
    PLoS Pathog 4:e1000200. 2008
  8. ncbi Mechanisms of asymmetric stem cell division
    Juergen A Knoblich
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3, 1030 Vienna, Austria
    Cell 132:583-97. 2008
  9. pmc Dynamics of adherens junctions in epithelial establishment, maintenance, and remodeling
    Buzz Baum
    University College London, London WC1E 6BT, England, UK
    J Cell Biol 192:907-17. 2011
  10. ncbi Cell polarity in eggs and epithelia: parallels and diversity
    Daniel St Johnston
    The Gurdon Institute and the Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Cell 141:757-74. 2010

Detail Information

Publications404 found, 100 shown here

  1. ncbi Cell migration: integrating signals from front to back
    Anne J Ridley
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London W1W 7BS, UK
    Science 302:1704-9. 2003
  2. ncbi Rho GTPases in cell biology
    Sandrine Etienne-Manneville
    MRC Laboratory for Molecular Cell Biology and Cell Biology Unit, Cancer Research UK Oncogene and Signal Transduction Group, University College London, Gower Street, London WC1E 6BT, UK
    Nature 420:629-35. 2002
    ..known principally for their pivotal role in regulating the actin cytoskeleton, but their ability to influence cell polarity, microtubule dynamics, membrane transport pathways and transcription factor activity is probably just as ..
  3. ncbi The cell biology of neurogenesis
    Magdalena Gotz
    Institute for Stem Cell Research, GSF National Research Center for Environment and Health, Ingolstadter Landstrasse 1, D 85764 Neuherberg Munich, Germany
    Nat Rev Mol Cell Biol 6:777-88. 2005
    ..Here, we discuss how these features change during development from neuroepithelial to radial glial cells, and how this transition affects cell fate and neurogenesis...
  4. ncbi Eukaryotic chemotaxis: a network of signaling pathways controls motility, directional sensing, and polarity
    Kristen F Swaney
    Department of Cell Biology, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA
    Annu Rev Biophys 39:265-89. 2010
  5. ncbi Epithelial cell polarity, stem cells and cancer
    Fernando Martin-Belmonte
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Madrid 28049, Spain
    Nat Rev Cancer 12:23-38. 2012
    ..Loss of expression or functional activity of cell adhesion and cell polarity proteins (including the PAR, crumbs (CRB) and scribble (SCRIB) complexes) is intricately related to advanced ..
  6. ncbi Tracking the ends: a dynamic protein network controls the fate of microtubule tips
    Anna Akhmanova
    Department of Cell Biology, Erasmus Medical Center, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Nat Rev Mol Cell Biol 9:309-22. 2008
    ..TIPs regulate different aspects of cell architecture by controlling microtubule dynamics, microtubule interactions with cellular structures and signalling factors, and the forces that are exerted on microtubule networks...
  7. pmc A sterol-regulatory element binding protein is required for cell polarity, hypoxia adaptation, azole drug resistance, and virulence in Aspergillus fumigatus
    Sven D Willger
    Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT, USA
    PLoS Pathog 4:e1000200. 2008
    ..role in ergosterol biosynthesis, resistance to the azole class of antifungal drugs, and in maintenance of cell polarity in A. fumigatus. Significantly, the SrbA null mutant was highly susceptible to fluconazole and voriconazole...
  8. ncbi Mechanisms of asymmetric stem cell division
    Juergen A Knoblich
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3, 1030 Vienna, Austria
    Cell 132:583-97. 2008
    ..These distinct pathways have been elucidated mostly in Drosophila. Although the molecules involved are highly conserved in vertebrates, the way they act is tissue specific and sometimes very different from invertebrates...
  9. pmc Dynamics of adherens junctions in epithelial establishment, maintenance, and remodeling
    Buzz Baum
    University College London, London WC1E 6BT, England, UK
    J Cell Biol 192:907-17. 2011
    ..This turnover contributes to junction formation and to the maintenance of epithelial integrity during tissue homeostasis and remodeling...
  10. ncbi Cell polarity in eggs and epithelia: parallels and diversity
    Daniel St Johnston
    The Gurdon Institute and the Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Cell 141:757-74. 2010
    b>Cell polarity, the generation of cellular asymmetries, is necessary for diverse processes in animal cells, such as cell migration, asymmetric cell division, epithelial barrier function, and morphogenesis...
  11. pmc Contact inhibition of locomotion in vivo controls neural crest directional migration
    Carlos Carmona-Fontaine
    Department of Anatomy and Developmental Biology, University College London, London WC1E 6BT, UK
    Nature 456:957-61. 2008
    ..These results provide the first example of contact inhibition of locomotion in vivo, provide an explanation for coherent directional migration of groups of cells and establish a previously unknown role for non-canonical Wnt signalling...
  12. pmc Rho-kinase/ROCK: A key regulator of the cytoskeleton and cell polarity
    Mutsuki Amano
    Department of Cell Pharmacology, Nagoya University, Showa Ku, Japan
    Cytoskeleton (Hoboken) 67:545-54. 2010
    ..This review focuses on the structure, function, and modes of activation and action of Rho-kinase...
  13. pmc Whole exome sequencing and homozygosity mapping identify mutation in the cell polarity protein GPSM2 as the cause of nonsyndromic hearing loss DFNB82
    Tom Walsh
    Department of Medicine, Division of Medical Genetics, University of Washington, Seattle, WA 98195, USA
    Am J Hum Genet 87:90-4. 2010
    ..an early truncation of the G protein signaling modulator GPSM2, a protein that is essential for maintenance of cell polarity and spindle orientation...
  14. pmc A molecular network for de novo generation of the apical surface and lumen
    David M Bryant
    Department of Anatomy, University of California, San Francisco, CA 94143 2140, USA
    Nat Cell Biol 12:1035-45. 2010
    ..This Rab11a-directed network directs Cdc42-dependent apical exocytosis during lumen formation, revealing an interaction between the machineries of vesicular transport and polarization...
  15. pmc Coordinated protein sorting, targeting and distribution in polarized cells
    Ira Mellman
    Genentech, Inc, 1 DNA Way, South San Francisco, California 94080, USA
    Nat Rev Mol Cell Biol 9:833-45. 2008
    ..The integration of these mechanisms into a complex and dynamic network is crucial for normal tissue function and is often defective in disease states...
  16. pmc Collective chemotaxis requires contact-dependent cell polarity
    Eric Theveneau
    Department of Cell and Developmental Biology, University College London, London WC1E 6BT, UK
    Dev Cell 19:39-53. 2010
    ..a mechanism of chemoattraction likely to function during both embryogenesis and cancer metastasis, whereby attractants such as Sdf1 amplify and stabilize contact-dependent cell polarity, resulting in directional collective migration.
  17. ncbi LKB1-dependent signaling pathways
    Dario R Alessi
    Medical Research Council, Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland
    Annu Rev Biochem 75:137-63. 2006
    ..functions as a tumor suppressor by not only inhibiting proliferation, but also by exerting profound effects on cell polarity and, most unexpectedly, on the ability of a cell to detect and respond to low cellular energy levels...
  18. ncbi Planar cell polarity links axes of spatial dynamics in neural-tube closure
    Tamako Nishimura
    RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Cell 149:1084-97. 2012
    ..We now provide evidence that planar cell polarity (PCP) signaling directly links these two processes...
  19. ncbi Epithelial organization, cell polarity and tumorigenesis
    Luke Martin McCaffrey
    Department of Oncology, Rosalind and Morris Goodman Cancer Research Centre, McGill University, Montreal, Canada
    Trends Cell Biol 21:727-35. 2011
    ..3D ex vivo culture systems can now faithfully recapitulate epithelial organ morphogenesis, providing a powerful approach to study both normal development and the initiating events in carcinogenesis...
  20. pmc Asymmetric cell divisions promote stratification and differentiation of mammalian skin
    Terry Lechler
    Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    Nature 437:275-80. 2005
    ..We further demonstrate that integrins and cadherins are essential for the apical localization of atypical protein kinase C, the Par3-LGN-Inscuteable complex and NuMA-dynactin to align the spindle...
  21. ncbi The extracellular matrix guides the orientation of the cell division axis
    Manuel Thery
    Biologie du cycle cellulaire et de la motilité, UMR144, CNRS, Institut Curie, 26 rue d Ulm 75248 Paris Cedex 05, France
    Nat Cell Biol 7:947-53. 2005
    ..In these cases, cell shape anisotropy and cell polarity affect spindle orientation...
  22. ncbi Cell flow reorients the axis of planar polarity in the wing epithelium of Drosophila
    Benoit Aigouy
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany
    Cell 142:773-86. 2010
    Planar cell polarity (PCP) proteins form polarized cortical domains that govern polarity of external structures such as hairs and cilia in both vertebrate and invertebrate epithelia...
  23. ncbi The transcriptional repressor ZEB1 promotes metastasis and loss of cell polarity in cancer
    Simone Spaderna
    Department of Visceral Surgery, University of Freiburg, Freiburg, Germany
    Cancer Res 68:537-44. 2008
    ..that the EMT-inducing transcriptional repressor ZEB1 promotes colorectal cancer cell metastasis and loss of cell polarity. Thereby, ZEB1 suppresses the expression of cell polarity factors, in particular of Lgl2, which we found ..
  24. ncbi Biology of the p21-activated kinases
    Gary M Bokoch
    Departments of Immunology and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Annu Rev Biochem 72:743-81. 2003
    ..We propose here a key role for PAK action in coordinating the dynamics of the actin and microtubule cytoskeletons during directional motility of cells, as well as in other functions requiring cytoskeletal polarization...
  25. pmc Calcium flickers steer cell migration
    Chaoliang Wei
    Institute of Molecular Medicine, State Key Laboratory of Biomembrane and Membrane Biotechnology, Peking University, Beijing 100871, China
    Nature 457:901-5. 2009
    ..These findings show how the exquisite spatiotemporal organization of calcium microdomains can orchestrate complex cellular processes such as cell migration...
  26. pmc Wnt9b signaling regulates planar cell polarity and kidney tubule morphogenesis
    Courtney M Karner
    Department of Internal Medicine, Division of Nephrology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Nat Genet 41:793-9. 2009
    ..Here we show that attenuation of Wnt9b signaling during kidney morphogenesis affects the planar cell polarity of the epithelium and leads to tubules with significantly increased diameter...
  27. pmc Strange as it may seem: the many links between Wnt signaling, planar cell polarity, and cilia
    John B Wallingford
    Howard Hughes Medical Institute, Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA
    Genes Dev 25:201-13. 2011
    ..and against a link between cilia and both the canonical Wnt/β-catenin pathway and the noncanonical Wnt/planar cell polarity (PCP) pathway. Furthermore, we address the evidence implicating a role for PCP components in ciliogenesis...
  28. ncbi Spindle orientation during asymmetric cell division
    Karsten H Siller
    Institute of Molecular Biology, Institute of Neuroscience, Howard Hughes Medical Institute, University of Oregon, Eugene, 97403, USA
    Nat Cell Biol 11:365-74. 2009
  29. ncbi Planar polarized actomyosin contractile flows control epithelial junction remodelling
    Matteo Rauzi
    IBDML, UMR6216 CNRS Université de la Méditerranée, Campus de Luminy, Case 907, 13288 Marseille Cedex 09, France
    Nature 468:1110-4. 2010
    ..Thus, epithelial morphogenesis is not properly reflected by Myosin II steady state distribution but by polarized contractile actomyosin flows that emerge from interactions between E-cadherin and actomyosin networks...
  30. pmc From cells to organs: building polarized tissue
    David M Bryant
    Department of Anatomy, University of California San Francisco, California 94143 2140, USA
    Nat Rev Mol Cell Biol 9:887-901. 2008
  31. ncbi Polar PIN localization directs auxin flow in plants
    Justyna Wisniewska
    Center for Plant Molecular Biology ZMBP, Tubingen University, D 72076 Tubingen, Germany
    Science 312:883. 2006
    ..This finding provides a crucial piece in the puzzle of how auxin flow can be redirected via rapid changes in PIN polarity...
  32. ncbi Regulation of cell polarity during epithelial morphogenesis
    Fernando Martin-Belmonte
    Centro de Biologia Molecular Severo Ochoa, CSIC, UAM, 28049 Madrid, Spain
    Curr Opin Cell Biol 20:227-34. 2008
    ..The polarity of individual cells is oriented and coordinated with other cells by interactions with the extracellular matrix...
  33. ncbi Flamingo, a seven-pass transmembrane cadherin, regulates planar cell polarity under the control of Frizzled
    T Usui
    Department of Biophysics, Faculty of Science, Japan
    Cell 98:585-95. 1999
    ..These results, together with phenotypes caused by ectopic expression of fz and fmi, suggest that cells acquire the P-D polarity by way of the Fz-dependent boundary localization of Fmi...
  34. pmc The PAR proteins: fundamental players in animal cell polarization
    Bob Goldstein
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Dev Cell 13:609-22. 2007
    ..Although the picture of how the PAR proteins function remains incomplete, cell biology and biochemistry are beginning to explain how PAR proteins polarize cells...
  35. pmc Actin dynamics counteract membrane tension during clathrin-mediated endocytosis
    Steeve Boulant
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Cell Biol 13:1124-31. 2011
    ..We also find that light-chain-bound Hip1R mediates actin engagement. These data thus provide a unifying explanation for the role of actin dynamics in coated-pit budding...
  36. pmc Principles of planar polarity in animal development
    Lisa V Goodrich
    Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
    Development 138:1877-92. 2011
    ..Here, we review the molecular mechanisms and cellular consequences of planar polarisation in diverse contexts, seeking to identify the common principles across the animal kingdom...
  37. ncbi Myosin-dependent junction remodelling controls planar cell intercalation and axis elongation
    Claire Bertet
    Laboratoire de Genetique et de Physiologie du Developpement, Institut de Biologie du Developpement de Marseille, CNRS Inserm Université de la Méditerranée, Campus de Luminy, Case 907, 13288 Marseille Cedex 9, France
    Nature 429:667-71. 2004
    ..This simple cellular mechanism provides a general model for polarized morphogenesis in epithelial organs...
  38. ncbi ZO-1 and ZO-2 independently determine where claudins are polymerized in tight-junction strand formation
    Kazuaki Umeda
    Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida Konoe, Kyoto 606 8501, Japan
    Cell 126:741-54. 2006
    ..When NZO-1 was forcibly recruited to lateral membranes and dimerized, claudins were dramatically polymerized. These findings indicate that ZO-1 and ZO-2 can independently determine whether and where claudins are polymerized...
  39. pmc Wnt signaling gradients establish planar cell polarity by inducing Vangl2 phosphorylation through Ror2
    Bo Gao
    National Human Genome Research Institute, Bethesda, MD 20892, USA
    Dev Cell 20:163-76. 2011
    ..Vangl2, a core component in planar cell polarity, forms Wnt-induced receptor complex with Ror2 to sense Wnt dosages...
  40. pmc Wnt7a activates the planar cell polarity pathway to drive the symmetric expansion of satellite stem cells
    Fabien Le Grand
    Sprott Center for Stem Cell Research, Ottawa Hospital Research Institute, Regenerative Medicine Program, 501 Smyth Road, Ottawa, ON K1H 8L6, Canada
    Cell Stem Cell 4:535-47. 2009
    ..Wnt7a signaling induced the polarized distribution of the planar cell polarity effector Vangl2. Silencing of Vangl2 inhibited Wnt7a action on satellite stem cell expansion...
  41. ncbi Collective cell migration
    Pernille Rørth
    Temasek Life Sciences Laboratory and Department of Biological Sciences, The National University of Singapore, Singapore
    Annu Rev Cell Dev Biol 25:407-29. 2009
    ..New properties that emerge from this connectivity can contribute to shaping, guiding, and ultimately ensuring tissue function...
  42. pmc Vertebrate kidney tubules elongate using a planar cell polarity-dependent, rosette-based mechanism of convergent extension
    Soeren S Lienkamp
    Department of Medicine, Renal Division, University of Freiburg Medical Center, Freiburg, Germany
    Nat Genet 44:1382-7. 2012
    ..In contrast to findings in Drosophila, however, non-canonical Wnt and planar cell polarity (PCP) signaling is required to control rosette topology and orientation during vertebrate kidney tubule ..
  43. pmc Planar cell polarity acts through septins to control collective cell movement and ciliogenesis
    Su Kyoung Kim
    Section of Molecular Cell and Developmental Biology and Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    Science 329:1337-40. 2010
    The planar cell polarity (PCP) signaling pathway governs collective cell movements during vertebrate embryogenesis, and certain PCP proteins are also implicated in the assembly of cilia...
  44. ncbi A spatial gradient coordinates cell size and mitotic entry in fission yeast
    James B Moseley
    The Rockefeller University, New York, New York 10065, USA
    Nature 459:857-60. 2009
    ..Here we show that the cell polarity protein kinase Pom1, which localizes to cell ends, regulates a signalling network that contributes to the ..
  45. ncbi Expression of crumbs confers apical character on plasma membrane domains of ectodermal epithelia of Drosophila
    A Wodarz
    Institut fur Entwicklungsbiologie, Universitat zu Koln, Federal Republic of Germany
    Cell 82:67-76. 1995
    ..Our results suggest that crumbs plays a key role in specifying the apical plasma membrane domain of ectodermal epithelial cells of Drosophila...
  46. pmc Membrane tension maintains cell polarity by confining signals to the leading edge during neutrophil migration
    Andrew R Houk
    Cardiovascular Research Institute and Department of Biochemistry, University of California San Francisco, San Francisco, CA 94143, USA
    Cell 148:175-88. 2012
  47. ncbi Pointing in the right direction: new developments in the field of planar cell polarity
    Roy Bayly
    Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA
    Nat Rev Genet 12:385-91. 2011
    Planar cell polarity (PCP) is observed in an array of developmental processes that involve collective cell movement and tissue organization, and its disruption can lead to severe developmental defects...
  48. ncbi Micropatterning as a tool to decipher cell morphogenesis and functions
    Manuel Thery
    Laboratoire de Physiologie Cellulaire et Végétale, iRTSV, CEA CNRS UJF INRA, 17 rue des Martyrs, 38054, Grenoble, France
    J Cell Sci 123:4201-13. 2010
    ..Remodelling of actin and microtubule networks participates in the adaptation of the entire cell polarity with respect to external constraints...
  49. pmc Collective cell migration requires suppression of actomyosin at cell-cell contacts mediated by DDR1 and the cell polarity regulators Par3 and Par6
    Cristina Hidalgo-Carcedo
    Tumour Cell Biology Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Nat Cell Biol 13:49-58. 2011
    ..Depletion of DDR1, Par3, Par6 or RhoE leads to increased actomyosin contactility at cell-cell contacts, a loss of cell-cell cohesion and defective collective cell invasion...
  50. pmc Neuronal polarity
    Sabina Tahirovic
    Max Planck Institute of Neurobiology, Axonal Growth and Regeneration, Am Klopferspitz 18, 82152 Martinsried, Germany
    Cold Spring Harb Perspect Biol 1:a001644. 2009
    ..e., to regenerate their axons after central nervous system (CNS) injury...
  51. pmc Mammalian Par3 regulates progenitor cell asymmetric division via notch signaling in the developing neocortex
    Ronald S Bultje
    Developmental Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA
    Neuron 63:189-202. 2009
    ..Here, we found that mammalian partition defective protein 3 (mPar3), a key cell polarity determinant, exhibits dynamic distribution in radial glial progenitors...
  52. ncbi The PAR-aPKC system: lessons in polarity
    Atsushi Suzuki
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    J Cell Sci 119:979-87. 2006
    ..over the past decade has shown that they are part of an evolutionarily conserved PAR-aPKC system involved in cell polarity in various biological contexts...
  53. ncbi Spontaneous cell polarization through actomyosin-based delivery of the Cdc42 GTPase
    Roland Wedlich-Soldner
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Science 299:1231-5. 2003
    ..form of Cdc42, a Rho-type guanosine triphosphatase (GTPase) required for cell polarization, could generate cell polarity without any recourse to a preestablished physical cue...
  54. ncbi Neuronal polarity: from extracellular signals to intracellular mechanisms
    Nariko Arimura
    Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University, 65, Tsurumai, Showa, Nagoya, Aichi 466 8550, Japan
    Nat Rev Neurosci 8:194-205. 2007
  55. ncbi Nuclear movement regulated by Cdc42, MRCK, myosin, and actin flow establishes MTOC polarization in migrating cells
    Edgar R Gomes
    Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032, USA
    Cell 121:451-63. 2005
    ..These results show that nuclear repositioning is an initial polarizing event in migrating cells and that the positions of the nucleus and the MTOC are established by separate regulatory pathways...
  56. ncbi Regulation of planar cell polarity by Smurf ubiquitin ligases
    Masahiro Narimatsu
    Center for Systems Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON, Canada, M5G 1X5
    Cell 137:295-307. 2009
    Planar cell polarity (PCP) is critical for morphogenesis in metazoans...
  57. ncbi Both the establishment and the maintenance of neuronal polarity require active mechanisms: critical roles of GSK-3beta and its upstream regulators
    Hui Jiang
    Institute of Neuroscience, Shanghai Institutes of Biological Sciences, The Graduate School, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China
    Cell 120:123-35. 2005
  58. ncbi Cooperative regulation of cell polarity and growth by Drosophila tumor suppressors
    D Bilder
    Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Science 289:113-6. 2000
    Loss of cell polarity and tissue architecture are characteristics of malignant cancers derived from epithelial tissues...
  59. pmc Rho-kinase directs Bazooka/Par-3 planar polarity during Drosophila axis elongation
    Sérgio de Matos Simões
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Avenue, New York, NY 10065, USA
    Dev Cell 19:377-88. 2010
    ..These results demonstrate that Rho-kinase plays an instructive role in planar polarity by targeting Baz/Par-3 and myosin II to complementary cortical domains...
  60. pmc A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae
    L Ni
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Mol Biol Cell 12:2147-70. 2001
    ..were identified, including those involved in actin-cytoskeleton organization, general bud site selection, cell polarity, vesicular transport, cell wall synthesis, protein modification, transcription, nuclear function, translation, ..
  61. ncbi Functionally unequal centrosomes drive spindle orientation in asymmetrically dividing Drosophila neural stem cells
    Elena Rebollo
    Cell Division Group, Institute for Research in Biomedicine, Parc Científic Barcelona, C Josep Samitier 1 5, 08028 Barcelona, Spain
    Dev Cell 12:467-74. 2007
    ..Apical aster maintenance requires the function of Pins. These results reveal that spindle orientation in Drosophila larval NBs is determined very early in the cell cycle, and is mediated by asymmetric centrosome function...
  62. pmc Generation of cell polarity in plants links endocytosis, auxin distribution and cell fate decisions
    Pankaj Dhonukshe
    Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
    Nature 456:962-6. 2008
    ..Efflux carriers for the signalling molecule auxin from the PIN family are landmarks of cell polarity in plants and have a crucial involvement in auxin distribution-dependent development including embryo ..
  63. pmc Differential regulation of protrusion and polarity by PI3K during neutrophil motility in live zebrafish
    Sa Kan Yoo
    Program in Cellular and Molecular Biology, University of Wisconsin Madison, Madison, WI 53706, USA
    Dev Cell 18:226-36. 2010
    b>Cell polarity is crucial for directed migration. Here we show that phosphoinositide 3-kinase (PI(3)K) mediates neutrophil migration in vivo by differentially regulating cell protrusion and polarity...
  64. ncbi Localization of apical epithelial determinants by the basolateral PDZ protein Scribble
    D Bilder
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 403:676-80. 2000
    ..Our results show that the lateral domain of epithelia, particularly the septate junction, functions in restricting apical membrane identity and correctly placing adherens junctions...
  65. ncbi Asymmetric T lymphocyte division in the initiation of adaptive immune responses
    John T Chang
    Abramson Family Cancer Research Institute and Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 315:1687-91. 2007
    ..These results suggest a mechanism by which a single lymphocyte can apportion diverse cell fates necessary for adaptive immunity...
  66. pmc Dynamics of core planar polarity protein turnover and stable assembly into discrete membrane subdomains
    Helen Strutt
    MRC Centre for Developmental and Biomedical Genetics, and Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Dev Cell 20:511-25. 2011
    ..Thus, we define the distinct roles of specific core proteins in the formation of asymmetric contacts between cells, which is a key event in the generation of coordinated cellular asymmetry...
  67. ncbi Polarization of PAR proteins by advective triggering of a pattern-forming system
    Nathan W Goehring
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Science 334:1137-41. 2011
    ..We suggest that passive advective transport in an active and flowing material may be a general mechanism for mechanochemical pattern formation in developmental systems...
  68. pmc The planar cell polarity gene Vangl2 is required for mammalian kidney-branching morphogenesis and glomerular maturation
    Laura L Yates
    Mammalian Genetics Unit, Medical Research Council, Harwell, Oxfordshire, UK
    Hum Mol Genet 19:4663-76. 2010
    The planar cell polarity (PCP) pathway, incorporating non-canonical Wnt signalling, controls embryonic convergent (CE) extension, polarized cell division and ciliary orientation...
  69. ncbi Cell polarity: quantitative modeling as a tool in cell biology
    Alex Mogilner
    Department of Neurobiology, Physiology, and Behavior, University of California, Davis, CA 95616, USA
    Science 336:175-9. 2012
    ..We review recent discoveries of cell polarity mechanisms, in which modeling played an important role, to demonstrate that the experiment-theory feedback ..
  70. ncbi Tumor suppressor PTEN mediates sensing of chemoattractant gradients
    Miho Iijima
    Department of Cell Biology, Johns Hopkins University, School of Medicine, 21205, Baltimore, MD, USA
    Cell 109:599-610. 2002
    ..These results suggest that specific phosphoinositides direct actin polymerization to the cell's leading edge and regulation of PTEN through a feedback loop plays a critical role in gradient sensing and directional migration...
  71. pmc Apical constriction: a cell shape change that can drive morphogenesis
    Jacob M Sawyer
    Biology Department, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Dev Biol 341:5-19. 2010
    ..Here, we review this effort to explore the diversity of mechanisms of apical constriction, the diversity of roles that apical constriction plays in development, and the common themes that emerge from comparing systems...
  72. pmc Asymmetric cell division: recent developments and their implications for tumour biology
    Juergen A Knoblich
    Institute of Molecular Biotechnology of Austrian Academy of Science, Doktor Bohr Gasse 3, 1030 Vienna, Austria
    Nat Rev Mol Cell Biol 11:849-60. 2010
    ..The relevance of asymmetric cell division for stem cell biology has added a new dimension to the field, and exciting connections between asymmetric cell division and tumorigenesis have begun to emerge...
  73. ncbi Rac1 orientates epithelial apical polarity through effects on basolateral laminin assembly
    T S Jou
    Department of Anatomy, Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA
    Nat Cell Biol 3:831-8. 2001
    ..These findings implicate Rac1-mediated laminin assembly in apical pole orientation. By linking apical orientation to generation of the basement membrane, epithelial cells ensure the coordination of polarity with tissue architecture...
  74. pmc Mechanistic mathematical model of polarity in yeast
    Natasha S Savage
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 23:1998-2013. 2012
    The establishment of cell polarity involves positive-feedback mechanisms that concentrate polarity regulators, including the conserved GTPase Cdc42p, at the "front" of the polarized cell...
  75. pmc Differential recruitment of Dishevelled provides signaling specificity in the planar cell polarity and Wingless signaling pathways
    J D Axelrod
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 USA
    Genes Dev 12:2610-22. 1998
    In Drosophila, planar cell polarity (PCP) signaling is mediated by the receptor Frizzled (Fz) and transduced by Dishevelled (Dsh). Wingless (Wg) signaling also requires Dsh and may utilize DFz2 as a receptor...
  76. pmc A comparison of mathematical models for polarization of single eukaryotic cells in response to guided cues
    Alexandra Jilkine
    Green Comprehensive Center for Computational and Systems Biology, Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Comput Biol 7:e1001121. 2011
  77. pmc Growth control by committee: intercellular junctions, cell polarity, and the cytoskeleton regulate Hippo signaling
    Julian C Boggiano
    Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA
    Dev Cell 22:695-702. 2012
    ..cell cortex-specifically the actin cytoskeleton, intercellular junctions, and protein complexes that determine cell polarity-as a key site for Hippo pathway regulation...
  78. pmc beta4 integrin-dependent formation of polarized three-dimensional architecture confers resistance to apoptosis in normal and malignant mammary epithelium
    Valerie M Weaver
    Department of Pathology and Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA
    Cancer Cell 2:205-16. 2002
    ..These results indicate that integrin-induced polarity may drive tumor cell resistance to apoptosis-inducing agents via effects on NFkappaB...
  79. ncbi Transcriptional regulation of cell polarity in EMT and cancer
    G Moreno-Bueno
    Departamento de Bioquimica, Universidad Autonoma de Madrid, Instituto de Investigaciones Biomedicas Alberto Sols, Consejo Superior de Investigaciones Cientificas Universidad Autonoma de Madrid, Madrid, Spain
    Oncogene 27:6958-69. 2008
    ..However, their effects on cell polarity remain unclear...
  80. pmc Integrin-mediated adhesion orients the spindle parallel to the substratum in an EB1- and myosin X-dependent manner
    Fumiko Toyoshima
    Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto, Japan
    EMBO J 26:1487-98. 2007
    ..Our results thus uncover the existence of a mechanism that orients the spindle parallel to the cell-substrate adhesion plane, and identify crucial factors involved in this novel mechanism...
  81. ncbi Directional migration of neural crest cells in vivo is regulated by Syndecan-4/Rac1 and non-canonical Wnt signaling/RhoA
    Helen K Matthews
    Department of Anatomy and Development Biology, University College London, Gower Street, London WC1E 6BT, UK
    Development 135:1771-80. 2008
    ..In addition, we show that RhoA inhibits Rac in NC cells. We present a model in which Syn4 and PCP control directional NC migration by, at least in part, regulating membrane protrusions through the regulation of small GTPase activities...
  82. pmc Regulation of protrusion, adhesion dynamics, and polarity by myosins IIA and IIB in migrating cells
    Miguel Vicente-Manzanares
    Department of Cell Biology, University of Virginia, Charlottesville, VA 22908
    J Cell Biol 176:573-80. 2007
    ..It mediates each of the major component processes that drive migration, e.g., polarization, protrusion, adhesion assembly and turnover, polarity, signaling, and tail retraction, and it integrates spatially separated processes...
  83. ncbi A complex between FAK, RACK1, and PDE4D5 controls spreading initiation and cancer cell polarity
    Bryan Serrels
    Edinburgh Cancer Research UK Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Crewe Road South, Edinburgh EH4 2XR, UK
    Curr Biol 20:1086-92. 2010
    ..The complex is recruited to nascent adhesions and promotes cell polarity. We identify FAK FERM domain residues whose mutation impairs RACK1 binding...
  84. ncbi GSK-3beta regulates phosphorylation of CRMP-2 and neuronal polarity
    Takeshi Yoshimura
    Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University, 65 Tsurumai, Showa Ku, Nagoya, Aichi 466 8550, Japan
    Cell 120:137-49. 2005
    ..Knockdown of CRMP-2 inhibited NT-3-induced axon outgrowth. These results suggest that NT-3 decreases phosphorylated CRMP-2 and increases nonphosphorylated active CRMP-2, thereby promoting axon outgrowth...
  85. ncbi The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42
    G Joberty
    Markey Center for Cell Signaling, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908 0577, USA
    Nat Cell Biol 2:531-9. 2000
    ..are essential for asymmetric cell division and polarized growth, whereas Cdc42 mediates establishment of cell polarity. Here we describe an unexpected link between these two systems...
  86. ncbi Neural stem cells: balancing self-renewal with differentiation
    Chris Q Doe
    Howard Hughes Medical Institute, Institute of Neuroscience, Institute of Molecular Biology, University of Oregon, Eugene, OR, USA
    Development 135:1575-87. 2008
  87. ncbi LKB1 and SAD kinases define a pathway required for the polarization of cortical neurons
    Anthony P Barnes
    Neuroscience Center, Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599, USA
    Cell 129:549-63. 2007
    ..Thus, we provide evidence in vivo and in vitro for a multikinase pathway that links extracellular signals to the intracellular machinery required for axon specification...
  88. pmc Planar cell polarity signaling: from fly development to human disease
    Matias Simons
    Mount Sinai School of Medicine, Department of Developmental and Regenerative Biology, New York, NY 10029, USA
    Annu Rev Genet 42:517-40. 2008
    ..In addition to the ubiquitous epithelial cell polarity along the apical-basolateral axis, many epithelial tissues and organs are also polarized within the plane of ..
  89. pmc Molecular regulation of lumen morphogenesis
    Anirban Datta
    Department of Anatomy, University of California San Francisco, CA 94143 2140, USA
    Curr Biol 21:R126-36. 2011
    ..We here discuss progress in understanding common design principles underpinning de novo lumen formation and expansion...
  90. ncbi Tissue/planar cell polarity in vertebrates: new insights and new questions
    Yanshu Wang
    Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Development 134:647-58. 2007
    This review focuses on the tissue/planar cell polarity (PCP) pathway and its role in generating spatial patterns in vertebrates...
  91. ncbi Roles of the fission yeast formin for3p in cell polarity, actin cable formation and symmetric cell division
    B Feierbach
    Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 W 168th Street, New York, NY 10032, USA
    Curr Biol 11:1656-65. 2001
    ..Formins are proteins with conserved roles in cell polarity, cytokinesis, and the regulation of actin and microtubule cytoskeletons.
  92. ncbi Control of tumourigenesis by the Scribble/Dlg/Lgl polarity module
    P O Humbert
    Cell Cycle and Cancer Genetics Laboratory, Research Division, Peter MacCallum Cancer Center, Melbourne, Victoria, Australia
    Oncogene 27:6888-907. 2008
    ..In Drosophila, these tumour suppressors function in a common genetic pathway to regulate apicobasal cell polarity and also play important roles in the control of cell proliferation, survival, differentiation and in cell ..
  93. ncbi Coupling between hydrodynamic forces and planar cell polarity orients mammalian motile cilia
    Boris Guirao
    Institut de Biologie de l Ecole Normale Supérieure IBENS, Institut National de la Santé et de la Recherche Médicale U1024, Centre National de la Recherche Scientifique UMR8197, 75005 Paris, France
    Nat Cell Biol 12:341-50. 2010
    ..and then reorient in a common direction through a coupling between hydrodynamic forces and the planar cell polarity (PCP) protein Vangl2, within a limited time-frame...
  94. pmc Cdc42 controls spindle orientation to position the apical surface during epithelial morphogenesis
    Aron B Jaffe
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    J Cell Biol 183:625-33. 2008
    ..We conclude that Cdc42 regulates epithelial tissue morphogenesis by controlling spindle orientation during cell division...
  95. pmc Mouse inscuteable induces apical-basal spindle orientation to facilitate intermediate progenitor generation in the developing neocortex
    Maria Pia Postiglione
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3 5, 1030 Vienna, Austria
    Neuron 72:269-84. 2011
    ..Our results indicate that the orientation of progenitor cell divisions is important for correct lineage specification in the developing mammalian brain...
  96. ncbi Munc13-4 is essential for cytolytic granules fusion and is mutated in a form of familial hemophagocytic lymphohistiocytosis (FHL3)
    Jerome Feldmann
    INSERM U429, Hopital Necker Enfants Malades, 75743 Paris, France
    Cell 115:461-73. 2003
    ..Expressed tagged hMunc13-4 localizes with cytotoxic granules at the immunological synapse. HMunc13-4 is therefore essential for the priming step of cytolytic granules secretion preceding vesicle membrane fusion...
  97. ncbi Cortical progenitor expansion, self-renewal and neurogenesis-a polarized perspective
    Simone A Fietz
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
    Curr Opin Neurobiol 21:23-35. 2011
    ..classes with regard to location of mitosis-apical and basal, and into three principal classes in terms of cell polarity during mitosis-bipolar, monopolar, and nonpolar...
  98. pmc Planar cell polarity: coordinating morphogenetic cell behaviors with embryonic polarity
    Ryan S Gray
    Department of Developmental Biology, Washington University School of Medicine in St Louis, St Louis, MO 63110, USA
    Dev Cell 21:120-33. 2011
    ..An evolutionarily conserved planar cell polarity (PCP) signaling system employs intra- and intercellular feedback interactions between its core components, ..
  99. pmc Symmetry breaking and the establishment of cell polarity in budding yeast
    Jayme M Johnson
    Duke University Medical Center, Durham, NC, USA
    Curr Opin Genet Dev 21:740-6. 2011
    b>Cell polarity is typically oriented by external cues such as cell-cell contacts, chemoattractants, or morphogen gradients...
  100. ncbi Antagonistic regulation of PIN phosphorylation by PP2A and PINOID directs auxin flux
    Marta Michniewicz
    Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, Tubingen, Germany
    Cell 130:1044-56. 2007
    In plants, cell polarity and tissue patterning are connected by intercellular flow of the phytohormone auxin, whose directional signaling depends on polar subcellular localization of PIN auxin transport proteins...
  101. ncbi Progress and challenges in understanding planar cell polarity signaling
    Jeffrey D Axelrod
    Department of Pathology, Stanford University School of Medicine, Room R226a, 300 Pasteur Drive, Stanford, CA 94305, USA
    Semin Cell Dev Biol 20:964-71. 2009
    ..This polarity, referred to as planar cell polarity (PCP), or tissue polarity, is essential for the normal physiological function of many epithelia...

Research Grants68

    Dennis Brown; Fiscal Year: 2006
    ..abstract_text> ..
  2. Identifying all Meckel-like ciliopathy genes by established total exome capture a
    Friedhelm Hildebrandt; Fiscal Year: 2010
    ..Centrosomal proteins regulate cell polarity and oriented cell division and participate in the mitotic spindle...
  3. Role of Endocytosis and Vesicular Trafficking in Podocyte Health and Filter Integ
    Puneet Garg; Fiscal Year: 2012
    ..on basic kidney biology, including applications within Cell and Molecular Biology of the Kidney (protein trafficking and cell polarity) and within the Pathobiology of Kidney Disease (normal biology of glomerular cells, and proteinuria).
  4. Misregulation of receptor tyrosine kinase signaling in PKD
    Jordan A Kreidberg; Fiscal Year: 2013
    ..membrane (cell-matrix adhesion) or with each other (cell-cell adhesion); (4) alterations in epithelial cell polarity;and (5) abnormal ciliary function...
  5. The Effects of Ethanol on Microtubules during Mouse Pre-implantation Development
    Yixian Zheng; Fiscal Year: 2012
    ..in vivo and in vitro mouse pre-implantation models, migration and cell-sorting assays, we will investigate how cell polarity and migration are affected by ethanol in mouse embryonic fibroblasts, mouse embryonic stem cells, and pre-..
  6. Developmental Physiology of Ciliated Epithelia
    ..This property of ciliated cells, called planar cell polarity, underlies the ability to produce long range flow that is directed along the appropriate tissue axis...
  7. Mammary Gland Morphogenesis and Breast Cancer
    Ian G Macara; Fiscal Year: 2013
    ..b>Cell polarity is essential for stem cell asymmetric divisions, and failures in polarization can result in tumorigenesis...
  8. Molecular and Cellular Regulation of Mammalian Renal Medulla Development
    Jing Yu; Fiscal Year: 2013
    ..Oriented cell division in the epithelium is a planar cell polarity (PCP) phenomenon, in which the Wnt/PCP pathway has been implicated...
  9. The role of the Wnt/planar cell polarity pathway in pulmonary angiogenesis
    Vinicio A de Jesus Perez; Fiscal Year: 2013
    ..of two Wnt signaling pathways in PAECs: the "canonical" Wnt/?-catenin (? C) and the "non-canonical" Wnt/planar cell polarity (PCP) pathways...
  10. Regulation of Apical-Basal Cell polarity during Intestinal Epithelial Morphogenes
    Nan Gao; Fiscal Year: 2013
    ..My long-term goal is to investigate the cell polarity regulators and their roles in the gastrointestinal morphogenesis and related diseases...
  11. Alcohol increases tight junction permeability &disrupts translocation of ZO-1
    Samantha Simet; Fiscal Year: 2012
    ..are characteristically located at the apical-lateral borders in airway epithelium where they regulate cell polarity and act to selectively regulate the passage of water, ions, neutral molecules and inflammatory cells through ..
  12. Using shared mechanisms of morphogenesis to study neural tube defects
    ..by applicant): Morphogenesis involves changes in cell shape regulated by complex networks that control cell polarity, cytoskeleton rearrangements and adhesive properties...
  13. Microtubule dynamics during cell polarity and migration
    Torsten Wittmann; Fiscal Year: 2012
    ..is decreased through phosphorylation by glycogen synthase kinase 3? (GSK3?), an emerging integrator of cell polarity pathways...
  14. Polarization and directed cell movements in engineered cellular environments
    Deborah E Leckband; Fiscal Year: 2010
    ..normal and malignant mammary epithelial cells, (ii) identify the impact of chemotactic gradients on epithelial cell polarity and migration, and (iii) determine how cadherin ligation and chemoattractants coordinately regulate the ..
  15. Modulation of Rab7-Dependent Degradative Pathways by Novel Adenovirus Protein
    Cathleen R Carlin; Fiscal Year: 2012
    ..nutrients to regulation of cell surface receptor expression and signal transduction, maintenance of cell polarity, and antigen presentation...
  16. Establishment of epithelial planar cell polarity
    Marek Mlodzik; Fiscal Year: 2013
    ..the ubiquitous apical-basal polarity and a second axis within the plane of the epithelium, called Planar Cell Polarity (PCP). Both polarity and ordered patterning are established during organogenesis...
  17. Targeted Improvement in Stem Cell Therapy for Leukemia and Bone Marrow Failure Sy
    Qishen Pang; Fiscal Year: 2013
    ..functions were associated with a decrease in the activity of the Rho GTPase Cdc42 known to be essential for cell polarity, adhesion and migration...
  18. Regulation of hair cell determination by the Wnt/Calcium signaling pathway
    Alain Dabdoub; Fiscal Year: 2012
    ..the two less-characterized noncanonical pathways: the Jun-N-terminal kinase pathway, also known as the planar cell polarity pathway, and the Wnt/Calcium/PKC pathway (Wodarz and Nusse, 1998;Huelsken and Birchmeier, 2001;Kuhl, 2004;..
  19. Morphogenesis of the organ of Corti
    Ping Chen; Fiscal Year: 2013
    ..periphery of the cochlea, displaying a polarity that is parallel to the sensory epithelium and known as planar cell polarity (PCP). The precise arrangement and polarity of hair cells is essential for auditory transduction...
  20. Mechanisms of Nuclear and Cell Fusion in Yeast
    MARK DAVID ROSE; Fiscal Year: 2013
    ..effectors of cell fusion, activated by Fus2p and Cdc42p, the highly conserved regulator of actin and cell polarity. We will use advanced imaging of membrane trafficking to identify the signaling mechanism(s) that regulate ..
  21. Role of Par1 Polarity Proteins in Podocyte Development and Glomerular Disease
    KIMBERLY JEAN REIDY; Fiscal Year: 2013
    ..Muesch, an Associate Professor in Development and Molecular Biology, is an expert in mechanisms that establish cell polarity, which will be examined in the context of glomerular disease...
  22. Mechanisms of Metal Ion Regulation in Human Cells
    Svetlana Lutsenko; Fiscal Year: 2013
    ..with complementary expertise in areas of structural biology, membrane transport, metal-ion coordination, cell polarity, and protein trafficking...
  23. Embryonic Development of the Mammalian Epididymis
    Barry T Hinton; Fiscal Year: 2013
    ..duct is novel, and we are especially interested in examining the contribution of a member of the planar cell polarity pathway (PCP), Ptk7, as a regulator of this important event during Wolffian duct morphogenesis...
  24. Genesis of Epithelial Cell Lumens
    Jennifer L Harder; Fiscal Year: 2013
    ..is to be an academic physician-scientist who is a leader in the field of mechanisms that control epithelial cell polarity. Her immediate goals are designed to facilitate her successful transition to independence: 1) learn to analyze ..
  25. A Novel Signaling Pathway in Planar Cell Polarity Establishment
    Marek Mlodzik; Fiscal Year: 2013
    ..As, such the information acquired in this application will both advance our understanding of PCP and organ patterning, and will also be of medical relevance in several disease contexts. ..
  26. Identification of novel genes as being important for neutrophil functions
    Dianqing Wu; Fiscal Year: 2013
    ..of cell polarization and directional cell migration for they are the fastest moving mammalian cells with overt cell polarity. Although a vast amount of work has been done, there are still many aspects of neutrophil biology that remain ..
  27. Oocyte polarity and mRNA localization in Zebrafish
    FLORENCE LOUISE MARLOW; Fiscal Year: 2013
    ..In vertebrates, the earliest indicator of cell polarity is an asymmetric aggregate, known as the Balbiani body, that includes organelles, proteins, and, in some ..
  28. Pathogenesis of TSC2-Related Tumors
    Raymond Yeung; Fiscal Year: 2012
    ..Since microtubules play an important role in long-distant transport, cell polarity, migration and cytokinesis, we hypothesize that mTOR may affect these processes via a microtubule-dependent ..
  29. The Roles of Fat3 and Atrophin-2 in Jaw-Joint Development in Zebrafish
    Pierre Le Pabic; Fiscal Year: 2013
    ..cell-cell communication as part of two systems that make cell populations behave as coherent units: the planar-cell polarity (PCP) pathway and the Hippo pathway...
  30. Pancreas Transcription Factors and Disease Model Systems
    Stephen F Konieczny; Fiscal Year: 2013
    ..in acinar cells, have provided the first insight into the molecular processes responsible for establishing cell polarity, protein synthesis functions, and maintenance of the acinar cell lineage...
    WOJCIECH A SWAT; Fiscal Year: 2012
    ..cells suggest that dlg and its homologs are also involved in establishing and/or maintaining epithelial cell polarity. We have studied mice lacking DLGH1 and found several urogenital defects, including sporadic renal agenesis, ..
    Steven L Teitelbaum; Fiscal Year: 2013
    ..Regulated secretion, also called exocytosis, requires cell polarity in which intracellular, cargo-containing vesicles are delivered, by organization of the cytoskeleton, to an ..
  33. Mechanism of ECM regulation of actin nucleation during morphogenesis.
    Martha C Soto; Fiscal Year: 2013
    Project Summary/Abstract The growth and spread of cancerous cells requires alterations in cell polarity, and in the organization of the actin cytoskeleton...
  34. The Control of Centriole Duplication and Degeneration
    Meng Fu Bryan Tsou; Fiscal Year: 2013
    ..The centrosome is essential for many cellular processes, including (i) formation of the mitotic spindle, (ii) cell polarity and asymmetric cell division, (iii) assembly of cilia and flagella, and (iv) suppression of parthenogenesis...
  35. Fat1 and Conventional Kinesin in Lateral Polarity
    DUNCAN BRUCE JOHNSTONE; Fiscal Year: 2012
    ..Fat1 research has applications to general cell biology, including cell polarity, intracellular protein transport, and protein-protein interactions...
  36. Regulation Of Cortical Neurogenesis By Apical Complex Proteins
    Seonhee Kim; Fiscal Year: 2013
    ..proliferation and the ultimate fate of cells in the cerebral cortex by regulating membrane architecture and cell polarity through interaction with apical complex proteins and AJ components...
  37. Regulation of Cell Polarity During Epidermal Growth and Homeostasis
    DANELLE N DEVENPORT; Fiscal Year: 2013
    The long-term objectives of this proposal are to understand the mechanisms that regulate cell polarity during epidermal growth and regeneration...
  38. Quantitative optical imaging of cilia-driven fluid flow
    MICHAEL ANDREW CHOMA; Fiscal Year: 2013
    ..relevant perturbations to ciliary physiology including increased fluid viscosity and altered planar cell polarity. Third, we will demonstrate intermediate defects in Xenopus and mouse ciliary function using quantitative ..
  39. Developmental Mechanisms of Vestibular Maculae Patterning in Mouse
    Michael R Deans; Fiscal Year: 2012
    ..This type of organization is called planar cell polarity (PCP) and has been described in a number of different tissues and species...
  40. Mechanisms of how nuclear envelope bridges link nuclei to the cytoskeleton.
    Daniel A Starr; Fiscal Year: 2013
    ..applicant: A wide variety of cellular processes, including fertilization, cell division, cell migration, and cell polarity, depend on nuclear migration events...
  41. Centrosomes and Hedgehog Signaling in Development and Polycystic Kidney Disease
    Hisham Bazzi; Fiscal Year: 2013
    ..We will also examine the roles of Sas4 in the cell cycle (especially mitosis), cell polarity and cell survival in the embryo...
  42. Regulation of Cytokinesis in Fission yeast.
    Dannel McCollum; Fiscal Year: 2013
    ..and SIN pathways play roles in cytokinesis and mitotic exit whereas the Ndr1/2 and MOR pathways function in cell polarity. In metazoans they have acquired additional functions in contact inhibition of growth, tumor suppression, and ..
  43. Planar Cell Polarity Signaling in Hair Follicle Formation
    Jiang Chen; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The planar cell polarity (PCP) signaling, or non-canonical Wnt (Wnt/PCP) signaling, is an evolutionarily conserved signaling pathway...
  44. Modulation of MUC1 Mucin Trafficking
    Rebecca P Hughey; Fiscal Year: 2010
    ..In tumors of epithelial origin, cell polarity is compromised and overexpressed MUC1 is found on all cell surfaces and inside the cell...
  45. Architecture of a Genetic Network Governing Morphogenesis
    David H A Fitch; Fiscal Year: 2013
    ..Such effectors have been identified in several model systems and include the cytoskeleton and determinants of cell polarity. The sex-, tissue-, position-, and time-specificity of morphogenesis is determined by "regulators", such as ..
  46. Growth regulation by the Fat signaling pathway
    Kenneth D Irvine; Fiscal Year: 2013
    ..to the vector and slope of Ds and Fj gradients to influence distinct downstream processes that affect planar cell polarity (PCP) and growth...
  47. IRS-1 and -2 signaling in mammary development and cancer
    Adrian V Lee; Fiscal Year: 2013
    ..These studies have raised three fundamental questions: 1) How do IRSs modulate mammary cell polarity, transformation, and tumorigenesis? 2) Why do both IRS-1 and IRS-2 cause transformation, but only IRS-2 is ..
  48. Regulation of Ciliated Cells that Control Cardiac Laterality
    Jeffrey D Amack; Fiscal Year: 2013
    ..that implicate three new pathways in the regulation of LR ciliated cells in KV: 1) Rho kinase signaling, 2) cell polarity and 3) ion pump-mediated ion flux...
  49. Dissecting Wnt/PCP Signaling in Developing Cochlear Sensory Epithelium
    Xiaowei Lu; Fiscal Year: 2012
    ..The uniform orientation of auditory hair bundles is a prominent example of epithelial planer cell polarity (PCP), or polarity within the plane of the epithelial sheet...
  50. Cell polarity and aging
    ..the mechanism responsible for this decline through evaluation of several pathways known to regulate cell polarity in budding yeast...
  51. Actions of the Sodium-Hydrogen Exchanger Subtype, NHE1
    Diane L Barber; Fiscal Year: 2012
    ..and structural biology to determine at the molecular level the regulation and function of pH sensors in cell polarity, actin-dependent membrane protrusion, and cell adhesion...
  52. Molecular characteristics of the apical recycling system
    ..This in vitro cell model can be evaluated for changes in microvillar assembly as well as alterations in cell polarity. In addition, we will be able to manipulate the phenotype in knockdown cells to evaluate particular Rab-..
  53. Regulation of in vivo cell migration by the polarity protein Par-1
    Jocelyn A McDonald; Fiscal Year: 2012
    ..Par-1 is a highly conserved serine-threonine kinase that functions in cell polarity, microtubule stability, and Wnt signaling, but has not been previously implicated in regulating cell migration...
  54. KRIT1 in Vascular Development and Dysfunction
    Kevin J Whitehead; Fiscal Year: 2013
    ..and a newly developed animal model of CCM disease in mice to explore the role of KRIT1 in RhoA activation and cell polarity in vascular development and disease...
  55. Planar Cell Polarity and the Cytoskeleton
    Andreas Jenny; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Planar cell polarity and the cytoskeleton Planar cell polarity (PCP) signaling regulates the establishment of polarity within the plane of an epithelium...
  56. Mechanisms of Cell Polarity Establishment
    Ian G Macara; Fiscal Year: 2013
    ..Par3 sits at the apex of a signaling network that controls cell polarity, and we discovered two crucial downstream functions of Par3 in epithelial cells - to deliver atypical protein ..
  57. Centrosome and mitotic functions of ciliary proteins
    Stephen Doxsey; Fiscal Year: 2009
    ..In nondividing cells of many organisms, centrosomes contribute to cell polarity and epithelial morphogenesis, and serve as templates for assembly of motile cilia and sensory (primary) cilia...
  58. PI(3,4,5)P3 Regulates Cell Polarity During Chemotaxis and Cytokinesis
    Christopher Janetopoulos; Fiscal Year: 2012
    ..2) Investigate the localization of important signaling proteins during cell polarity and find new components with novel genetic screens...
  59. Wnt5a and Planar Cell Polarity in Kidney Development and Cyst formation
    Liwei Huang; Fiscal Year: 2013
    ..Successful completion of these experiments will result in the identification of novel candidate targets for therapeutic intervention in ADPKD, a disease for which no approved treatments exist. ..
  60. Signals Regulating SAG Development
    James A Marrs; Fiscal Year: 2010
    ..Normal SAG development will be compared with cadherin loss-of-function phenotypes. In addition, cell polarity signaling molecule functions during SAG development will be studied. Aim 3...
  61. 2009 Motile and Contractile Systems Gordon Research Conference
    Kerry S Bloom; Fiscal Year: 2013
    ..making central contributions to cell motility, cell signaling, membrane trafficking, cell division, cell polarity, and the development of multicellular organisms...
    ..no studies have examined how MARK2- dependent phosphorylation events could influence intestinal epithelial cell polarity. In intestinal cells, we also have demonstrated that loss of Rab25, an epithelial-specific small GTP binding ..
  63. The Role of p120 Catenin in Metanephric Kidney Development
    DENISE K MARCIANO; Fiscal Year: 2012
    ..of p120 catenin results in polycystic kidneys due to an alteration of cellular proliferation, apoptosis or cell polarity. This will be accomplished by biochemical and histological methods in mice lacking p120 catenin from nephron ..
    Graham Thomas; Fiscal Year: 2001
    ..been postulated that regional variation in the membrane skeleton may participate in the process of generating cell polarity in non-erythroid tissues, the specific roles of the various spectrin isoforms with distinct tissue and ..
    Lopa Mishra; Fiscal Year: 1999
    ..Associated Guanylate Kinase), which by homology studies appear to be important in the establishment of cell polarity, and to establish a basis for their role in hepatic differentiation...
  66. Autocrine control of neutrophil chemotaxis
    Wolfgang G Junger; Fiscal Year: 2010
    ..adenosine formed from the released ATP controls negative feedback loops that play a critical role in defining cell polarity and uropod retraction...
  67. Proteomic approach to identify mediators of PI3K activation by P. aeruginosa
    Joanne N Engel; Fiscal Year: 2010
    ..In the setting of epithelial injury and loss of cell polarity PA can effectively colonize the mucosal surfaces, cause further damage, and disseminate...
    PHILIP BREITFELD; Fiscal Year: 1990
    ..cells, and in particular hepatocytes, serve as a useful model to study the development and maintenance of cell polarity since hepatocytes display morphologic polarity in monolayer culture...