cell membrane permeability


Summary: A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.

Top Publications

  1. ncbi Water permeation across biological membranes: mechanism and dynamics of aquaporin-1 and GlpF
    B L de Groot
    Theoretical Molecular Biophysics Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Science 294:2353-7. 2001
  2. pmc Molecular basis of bacterial outer membrane permeability revisited
    Hiroshi Nikaido
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3202, USA
    Microbiol Mol Biol Rev 67:593-656. 2003
  3. ncbi The tight junction: a multifunctional complex
    Eveline E Schneeberger
    Molecular Pathology Unit, Massachusetts General Hospital East, Charlestown, MA 02129, USA
    Am J Physiol Cell Physiol 286:C1213-28. 2004
  4. pmc Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice
    Mikio Furuse
    Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 156:1099-111. 2002
  5. pmc Twenty years of cell-penetrating peptides: from molecular mechanisms to therapeutics
    Frederic Heitz
    Centre de Recherches de Biochimie Macromoleculaire, UMR 5237, CNRS, UM 1, UM 2, CRBM Department of Molecular Biophysics and Therapeutics, 1919 Route de Mende, Montpellier, France
    Br J Pharmacol 157:195-206. 2009
  6. ncbi Structural determinants of water permeation through aquaporin-1
    K Murata
    National Institute for Physiological Sciences, Okazaki, Japan
    Nature 407:599-605. 2000
  7. ncbi Regulation of tight junction permeability by intestinal bacteria and dietary components
    Dulantha Ulluwishewa
    Food Nutrition Genomics Team, Agri Foods and Health Section, Palmerston North 4442, New Zealand
    J Nutr 141:769-76. 2011
  8. pmc ZO-1 stabilizes the tight junction solute barrier through coupling to the perijunctional cytoskeleton
    Christina M Van Itallie
    Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Biol Cell 20:3930-40. 2009
  9. pmc Occludin-deficient embryonic stem cells can differentiate into polarized epithelial cells bearing tight junctions
    M Saitou
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Cell Biol 141:397-408. 1998
  10. ncbi Recent advances in the use of cell-penetrating peptides for medical and biological applications
    Sonali B Fonseca
    Department of Pharmaceutical Sciences, University of Toronto, Ontario, Canada
    Adv Drug Deliv Rev 61:953-64. 2009

Research Grants

Detail Information

Publications325 found, 100 shown here

  1. ncbi Water permeation across biological membranes: mechanism and dynamics of aquaporin-1 and GlpF
    B L de Groot
    Theoretical Molecular Biophysics Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Science 294:2353-7. 2001
    ..In AQP1, a fine-tuned water dipole rotation during passage is essential for water selectivity. In GlpF, a glycerol-mediated "induced fit" gating motion is proposed to generate selectivity for glycerol over water...
  2. pmc Molecular basis of bacterial outer membrane permeability revisited
    Hiroshi Nikaido
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3202, USA
    Microbiol Mol Biol Rev 67:593-656. 2003
  3. ncbi The tight junction: a multifunctional complex
    Eveline E Schneeberger
    Molecular Pathology Unit, Massachusetts General Hospital East, Charlestown, MA 02129, USA
    Am J Physiol Cell Physiol 286:C1213-28. 2004
  4. pmc Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice
    Mikio Furuse
    Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 156:1099-111. 2002
    ..These findings provide the first evidence that continuous claudin-based TJs occur in the epidermis and that these TJs are crucial for the barrier function of the mammalian skin...
  5. pmc Twenty years of cell-penetrating peptides: from molecular mechanisms to therapeutics
    Frederic Heitz
    Centre de Recherches de Biochimie Macromoleculaire, UMR 5237, CNRS, UM 1, UM 2, CRBM Department of Molecular Biophysics and Therapeutics, 1919 Route de Mende, Montpellier, France
    Br J Pharmacol 157:195-206. 2009
    ..We will also highlight the application of peptide carriers for the delivery of therapeutic molecules and provide an update of their clinical evaluation...
  6. ncbi Structural determinants of water permeation through aquaporin-1
    K Murata
    National Institute for Physiological Sciences, Okazaki, Japan
    Nature 407:599-605. 2000
    ..The atomic model provides a possible molecular explanation to a longstanding puzzle in physiology-how membranes can be freely permeable to water but impermeable to protons...
  7. ncbi Regulation of tight junction permeability by intestinal bacteria and dietary components
    Dulantha Ulluwishewa
    Food Nutrition Genomics Team, Agri Foods and Health Section, Palmerston North 4442, New Zealand
    J Nutr 141:769-76. 2011
    ..Various dietary components are also known to regulate epithelial permeability by modifying expression and localization of TJ proteins...
  8. pmc ZO-1 stabilizes the tight junction solute barrier through coupling to the perijunctional cytoskeleton
    Christina M Van Itallie
    Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Biol Cell 20:3930-40. 2009
    ..These studies are the first direct evidence that ZO-1 limits solute permeability in established tight junctions, perhaps by forming a stabilizing link between the barrier and perijunctional actomyosin...
  9. pmc Occludin-deficient embryonic stem cells can differentiate into polarized epithelial cells bearing tight junctions
    M Saitou
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Cell Biol 141:397-408. 1998
    ..These findings indicate that there are as yet unidentified TJ integral membrane protein(s) which can form strand structures, recruit ZO-1, and function as a barrier without occludin...
  10. ncbi Recent advances in the use of cell-penetrating peptides for medical and biological applications
    Sonali B Fonseca
    Department of Pharmaceutical Sciences, University of Toronto, Ontario, Canada
    Adv Drug Deliv Rev 61:953-64. 2009
    ..This review will illustrate the diverse applications for which CPPs have been harnessed and also demonstrate the remarkable advancements these peptides have facilitated in cell biology...
  11. pmc The mitochondrial permeability transition pore and its role in cell death
    M Crompton
    Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, U K
    Biochem J 341:233-49. 1999
    ..Current evidence that the pore complex is involved in outer-membrane rupture and release of these proteins during programmed cell death is reviewed, along with indications that transient pore opening may provoke 'accidental' apoptosis...
  12. ncbi Mechanisms of antimicrobial peptide action and resistance
    Michael R Yeaman
    Division of Infectious Diseases, Harbor University of California Los Angeles Medical Center, Torrance, California, USA
    Pharmacol Rev 55:27-55. 2003
    ..From these perspectives, the intention of this review is to illustrate the contemporary structural and functional themes among mechanisms of antimicrobial peptide action and resistance...
  13. ncbi TNF-alpha-induced increase in intestinal epithelial tight junction permeability requires NF-kappa B activation
    Thomas Y Ma
    Department of Internal Medicine, University of New Mexico, Albuquerque, NM 87131 0001, USA
    Am J Physiol Gastrointest Liver Physiol 286:G367-76. 2004
    ..The increase in permeability was associated with NF-kappa B-dependent downregulation of ZO-1 protein expression and alteration in junctional localization...
  14. ncbi Sensitization to the lysosomal cell death pathway by oncogene-induced down-regulation of lysosome-associated membrane proteins 1 and 2
    Nicole Fehrenbacher
    Apoptosis Department and Centre for Genotoxic Stress Response, Institute for Cancer Biology, Danish Cancer Society, Copenhagen, Denmark
    Cancer Res 68:6623-33. 2008
    ..These data indicate that aggressive cancers with high cysteine cathepsin levels are especially sensitive to lysosomal cell death pathways and encourage the further development of lysosome-targeting compounds for cancer therapy...
  15. ncbi Quaternary ammoniums and other preservatives' contribution in oxidative stress and apoptosis on Chang conjunctival cells
    C Debbasch
    Unit of Cellular Pharmacotoxicology, Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, the Toxicology Laboratory, University of Paris-V, France
    Invest Ophthalmol Vis Sci 42:642-52. 2001
    ..Superoxide anions may play an important role in tissue damage induced by preservatives in ocular surface disorders...
  16. ncbi Membrane permeabilization and cell damage by ultrashort electric field shocks
    Andrei G Pakhomov
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA
    Arch Biochem Biophys 465:109-18. 2007
    ..We conclude that plasma membrane permeabilization is the principal cause of necrotic transformation in nsPEF-exposed cells and probably contributes to other known nsPEF bioeffects...
  17. ncbi Ethanol modulation of intestinal epithelial tight junction barrier
    T Y Ma
    Division of Gastroenterology, Department of Medicine, Department of Veterans Affairs Medical Center, Long Beach 90822, USA
    Am J Physiol 276:G965-74. 1999
    ..The results of this study indicate that ethanol at low noncytotoxic doses causes a functional and structural opening of the Caco-2 intestinal epithelial TJ barrier by activating MLCK...
  18. ncbi Practical applications and feasibility of efflux pump inhibitors in the clinic--a vision for applied use
    Olga Lomovskaya
    Mpex Pharmaceuticals Inc, 3030 Bunker Hill Street, San Diego, CA 92109, USA
    Biochem Pharmacol 71:910-8. 2006
    ..It is also apparent that some changes in regulatory decision-making regarding resistance would be very helpful in order to facilitate approval of agents aiming to reverse resistance and prevent its further development...
  19. ncbi Myosin light chain phosphorylation regulates barrier function by remodeling tight junction structure
    Le Shen
    Department of Pathology, The University of Chicago, 5841 South Maryland Avenue, MC 1089, Chicago, IL 60637, USA
    J Cell Sci 119:2095-106. 2006
    ..We conclude that myosin light chain phosphorylation alone is sufficient to induce tight junction regulation and provide new insights into the molecular mechanisms that mediate this regulation...
  20. pmc Relative CO2/NH3 selectivities of AQP1, AQP4, AQP5, AmtB, and RhAG
    Raif Musa-Aziz
    Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 106:5406-11. 2009
    ..Thus, each channel exhibits a characteristic ratio for indices of CO(2) vs. NH(3) permeability, demonstrating that, like ion channels, gas channels can exhibit selectivity...
  21. ncbi Antimicrobial peptides in action
    Hari Leontiadou
    Department of Biophysical Chemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Am Chem Soc 128:12156-61. 2006
    ..The remaining peptides lay close to the edge of the pore, maintaining a predominantly parallel orientation with respect to the membrane...
  22. pmc Triton X-100 concentration effects on membrane permeability of a single HeLa cell by scanning electrochemical microscopy (SECM)
    Dipankar Koley
    Center for Electrochemistry, Department of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station, A5300, Austin, TX 78712 0165, USA
    Proc Natl Acad Sci U S A 107:16783-7. 2010
    ..19 to 0.20 mM. The impermeability of ferrocyanide molecules in the absence of surfactant was also used to determine the height and diameter of a single living cell with the aid of the approach curve and probe scan methods in SECM...
  23. ncbi The apoptotic microtubule network preserves plasma membrane integrity during the execution phase of apoptosis
    José A Sánchez-Alcázar
    Centro Andaluz de Biologia del Desarrollo, Universidad Pablo de Olavide CSIC, Carretera de Utrera Km 1, Sevilla, 41013, Spain
    Apoptosis 12:1195-208. 2007
    ..We propose that apoptotic microtubule network is required to maintain plasma membrane integrity during the execution phase of apoptosis...
  24. ncbi Cofilin mediates tight-junction opening by redistributing actin and tight-junction proteins
    Yoko Nagumo
    Graduate School of Life and Environmental Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba, Ibaraki 305 8572, Japan
    Biochem Biophys Res Commun 377:921-5. 2008
    ..These results strongly support the importance of cofilin in TJ opening, suggesting cofilin as a target for TJ permeability regulation in epithelial cells...
  25. pmc Permeability of homotypic and heterotypic gap junction channels formed of cardiac connexins mCx30.2, Cx40, Cx43, and Cx45
    Mindaugas Rackauskas
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Am J Physiol Heart Circ Physiol 293:H1729-36. 2007
    ..2 than for Cx40, Cx43, and Cx45, suggesting that mCx30.2 GJs are notably more adapted to perform electrical rather than metabolic cell-cell communication...
  26. ncbi Lysosomal membrane permeabilization in cell death
    P Boya
    3D Lab Development, Differentiation and Degeneration, Department of Cellular and Molecular Physiopathology, Centro de Investigaciones Biologicas, CSIC, Madrid, Spain
    Oncogene 27:6434-51. 2008
    ..The regulation of LMP is perturbed in cancer cells, suggesting that specific strategies for LMP induction might lead to novel therapeutic avenues...
  27. pmc Interaction of p190RhoGAP with C-terminal domain of p120-catenin modulates endothelial cytoskeleton and permeability
    Noureddine Zebda
    Lung Injury Center, Section of Pulmonary and Critical Medicine, Department of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 288:18290-9. 2013
  28. ncbi Over-expression of the Arabidopsis AtMYB41 gene alters cell expansion and leaf surface permeability
    Eleonora Cominelli
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy
    Plant J 53:53-64. 2008
    ..Taken together, our results suggest that AtMYB41 is part of a complex network of transcription factors controlling cell expansion and cuticle deposition in response to abiotic stress...
  29. pmc Analysis of aquaporin-mediated diffusional water permeability by coherent anti-stokes Raman scattering microscopy
    Keiji Ibata
    Laboratory for Cell Function Dynamics, Brain Science Institute, The Institute of Physical and Chemical Research RIKEN, Saitama, Japan
    Biophys J 101:2277-83. 2011
    ..These results suggest the possibility of using CARS imaging to investigate the hydrodynamics of single mammalian cells as well as the regulation of AQPs...
  30. ncbi Understanding efflux in Gram-negative bacteria: opportunities for drug discovery
    Herbert P Schweizer
    Colorado State University, IDRC at Foothills Campus, Department of Microbiology, Immunology and Pathology, Fort Collins, CO 80523 0922, USA
    Expert Opin Drug Discov 7:633-42. 2012
    ..However, recent advances in our understanding of efflux in these bacteria provide opportunities and assets for drug discovery...
  31. ncbi Screening the cytotoxicity of single-walled carbon nanotubes using novel 3D tissue-mimetic models
    Dania Movia
    Centre for Research on Adaptive Nanostructures and Nanodevices CRANN, Trinity College Dublin, Ireland
    ACS Nano 5:9278-90. 2011
  32. pmc Differential effects of TNF (TNFSF2) and IFN-γ on intestinal epithelial cell morphogenesis and barrier function in three-dimensional culture
    Kati Juuti-Uusitalo
    Top Institute Food and Nutrition, Wageningen, The Netherlands
    PLoS ONE 6:e22967. 2011
    ..Apoptosis and tight junctions are also important for epithelial tissue morphogenesis, but the effect of TNF and IFNγ on the process of intestinal epithelial morphogenesis is unknown...
  33. pmc Low-resolution structure of a vesicle disrupting α-synuclein oligomer that accumulates during fibrillation
    Lise Giehm
    Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmark
    Proc Natl Acad Sci U S A 108:3246-51. 2011
    ..This is confirmed by the ability of the purified oligomer to disrupt liposomes. Our results provide the first structural description in solution of a potentially cytotoxic oligomer, which accumulates during the fibrillation of αSN...
  34. pmc Permeation of styryl dyes through nanometer-scale pores in membranes
    Yao Wu
    Howard Hughes Medical Institute and Department of Neuroscience, University of Wisconsin, Madison, Wisconsin 53706, United States
    Biochemistry 50:7493-502. 2011
    ..Together, the data reported here help provide a framework for interpreting dye destaining rates from secretory vesicles...
  35. ncbi Expression and regulation of toll-like receptors in cerebral endothelial cells
    Péter Nagyoszi
    Institute of Biophysics, Biological Research Center, Szeged, Hungary
    Neurochem Int 57:556-64. 2010
    ..Changes in occludin expression and localization could be inhibited by the ERK1/2 inhibitor U0126. Our results suggest a significant role of the cerebral endothelium in mediation of the neural effects of different inflammatory processes...
  36. pmc Activation of stress-related signalling pathway in human cells upon SiO2 nanoparticles exposure as an early indicator of cytotoxicity
    Bashir Mustafa Mohamed
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin 8, Ireland
    J Nanobiotechnology 9:29. 2011
    ..Thus, a mechanistic and systematic evaluation of the potential biological and toxic effects of SiO2NP becomes crucial in order to assess their complete safe applicability limits...
  37. pmc Vascular permeability to plasma, plasma proteins, and cells: an update
    Harold F Dvorak
    Center for Vascular Biology Research and the Department of Pathology, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA
    Curr Opin Hematol 17:225-9. 2010
    ..The mechanisms responsible for these events have been the subject of intense investigation and, often, dispute...
  38. pmc Surfactant proteins A and D inhibit the growth of Gram-negative bacteria by increasing membrane permeability
    Huixing Wu
    Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio, USA
    J Clin Invest 111:1589-602. 2003
  39. pmc Effects of ethanol and acetaldehyde on tight junction integrity: in vitro study in a three dimensional intestinal epithelial cell culture model
    Elhaseen Elamin
    Top Institute Food and Nutrition, Wageningen, The Netherlands
    PLoS ONE 7:e35008. 2012
  40. pmc Modeling transmembrane transport through cell membrane wounds created by acoustic cavitation
    Vladimir Zarnitsyn
    School of Chemical and Biomolecular Engineering, and Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA
    Biophys J 95:4124-38. 2008
    ..Overall, this study developed a theoretical framework for analysis of transmembrane transport through cell membrane wounds and thereby provided quantitative estimates of their size and lifetime...
  41. ncbi Cell penetrating peptides: overview and applications to the delivery of oligonucleotides
    F Said Hassane
    UMR 5235 CNRS, Dynamique des Interactions Membranaires Normales et Pathologiques, Universite Montpellier 2, 34095, Montpellier Cedex 5, France
    Cell Mol Life Sci 67:715-26. 2010
    ..In many instances, however, endosomal escape appears as a major limitation of this new delivery strategy...
  42. pmc Structural context shapes the aquaporin selectivity filter
    David F Savage
    Graduate Group in Biophysics, Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158 2517, USA
    Proc Natl Acad Sci U S A 107:17164-9. 2010
  43. ncbi The rise of PAMPA
    Alex Avdeef
    pION INC, 5 Constitution Way, Woburn, MA 01801, USA
    Expert Opin Drug Metab Toxicol 1:325-42. 2005
    ..The review concludes with a binning strategy to predict human intestinal absorption, based on the use of the sum of permeability coefficients, measured at gradient pH 5.0, 6.2 and 7.4. Opinions regarding the future of PAMPA are offered...
  44. ncbi Permeability of mouse oocytes and embryos at various developmental stages to five cryoprotectants
    Prudencio B Pedro
    Laboratory of Animal Science, College of Agriculture, Kochi University, Japan
    J Reprod Dev 51:235-46. 2005
    ..Such information will be valuable for determining a suitable protocol for the cryopreservation of oocytes/embryos at different stages of development...
  45. ncbi Fluconazole modulates membrane rigidity, heterogeneity, and water penetration into the plasma membrane in Saccharomyces cerevisiae
    Fumiyoshi Abe
    Molecular Evolution and Adaptation Research, Institute of Biogeosciences, Japan Agency for Marine Earth Science and Technology JAMSTEC, Yokosuka 237 0061, Japan
    Biochemistry 48:8494-504. 2009
    ..Our findings illustrate the role of ergosterol in maintaining membrane heterogeneity and preventing water penetration as well as maintaining the rigidity of the plasma membrane interfacial region...
  46. pmc Selective cytotoxicity of intense nanosecond-duration electric pulses in mammalian cells
    Bennett L Ibey
    Radio Frequency Radiation Branch, 711th Human Performance Wing, Air Force Research Laboratory, Brooks City Base, San Antonio, TX, USA
    Biochim Biophys Acta 1800:1210-9. 2010
    ..We explored the cytotoxic effect of 10-ns EP (quantitation, mechanisms, efficiency, and specificity) in comparison with 300-ns, 1.8- and 9-μs EP...
  47. pmc Tricellulin forms a barrier to macromolecules in tricellular tight junctions without affecting ion permeability
    Susanne M Krug
    Institute of Clinical Physiology, Charite, Freie Universitat, Berlin, Germany
    Mol Biol Cell 20:3713-24. 2009
    ..In conclusion, at low tricellulin expression the tTJ central tube forms a pathway for macromolecules. At higher expression, tricellulin forms a barrier in tTJs effective only for macromolecules and in bTJs for solutes of all sizes...
  48. ncbi The structure of a Staphylococcus aureus leucocidin component (LukF-PV) reveals the fold of the water-soluble species of a family of transmembrane pore-forming toxins
    J D Pedelacq
    Groupe de Cristallographie Biologique Institut de Pharmacologie et de Biologie Structurale du CNRS 205 route de Narbonne 31077 Toulouse Cedex France
    Structure 7:277-87. 1999
    ..The components of each protein class are produced as non-associated, water-soluble proteins that undergo conformational changes and oligomerization after recognition of their cell targets...
  49. ncbi Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers
    Ina Hubatsch
    Department of Pharmacy, Uppsala University, Uppsala, Sweden
    Nat Protoc 2:2111-9. 2007
    ..The permeability assay can be completed within one working day, provided that the Caco-2 monolayers have been cultured and differentiated on the permeable supports 3 weeks in advance...
  50. ncbi Nanopore formation and phosphatidylserine externalization in a phospholipid bilayer at high transmembrane potential
    P Thomas Vernier
    MOSIS, Information Sciences Institute, Viterbi School of Engineering, University of Southern California, Marina del Rey, California 90292, USA
    J Am Chem Soc 128:6288-9. 2006
  51. ncbi Sonoporation by ultrasound-activated microbubble contrast agents: effect of acoustic exposure parameters on cell membrane permeability and cell viability
    Raffi Karshafian
    Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada
    Ultrasound Med Biol 35:847-60. 2009
    ..The results indicate that ultrasound exposure parameters can be optimized for therapeutic sonoporation and that bubble disruption is a necessary but insufficient indicator of ultrasound-induced permeabilization...
  52. ncbi Temporins, small antimicrobial peptides with leishmanicidal activity
    Maria Luisa Mangoni
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Scienze Biochimiche A Rossi Fanelli, Azienda Ospedaliera S Andrea, Italy
    J Biol Chem 280:984-90. 2005
  53. ncbi Brain pericytes contribute to the induction and up-regulation of blood-brain barrier functions through transforming growth factor-beta production
    Shinya Dohgu
    Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, 8 19 1 Nanakuma, Jonan ku, Fukuoka 814 0180, Japan
    Brain Res 1038:208-15. 2005
    ..These findings suggest that brain pericytes contribute to the up-regulation of BBB functions through continuous TGF-beta production...
  54. ncbi Amyloid-beta-induced occludin down-regulation and increased permeability in human brain endothelial cells is mediated by MAPK activation
    L M Tai
    Department of Life Sciences, The Open University, Milton Keynes, UK
    J Cell Mol Med 14:1101-12. 2010
    ..Our findings suggest that the JNK and p38MAPK pathways might represent attractive therapeutic targets for preventing BBB dysfunction in AD...
  55. ncbi Carrier-mediated cellular uptake of pharmaceutical drugs: an exception or the rule?
    Paul D Dobson
    School of Chemistry and Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
    Nat Rev Drug Discov 7:205-20. 2008
  56. pmc Differential effects of hydrocortisone and TNFalpha on tight junction proteins in an in vitro model of the human blood-brain barrier
    Carola Förster
    Institute of Anatomy and Cell Biology, University of Wurzburg, Koellikerstrasse 6, D 97070 Wurzburg, Germany
    J Physiol 586:1937-49. 2008
    ..Our studies strongly suggest stabilization of BBB function as a mode of GC action on a molecular level in the human brain vasculature...
  57. pmc Physical effects underlying the transition from primitive to modern cell membranes
    Itay Budin
    Howard Hughes Medical Institute, and Department of Molecular Biology, and the Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 108:5249-54. 2011
    ..The evolution of phospholipid membranes could thus have been a deterministic outcome of intrinsic physical processes and a key driving force for early cellular evolution...
  58. pmc Pharmacological characterization of pannexin-1 currents expressed in mammalian cells
    Weihong Ma
    Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    J Pharmacol Exp Ther 328:409-18. 2009
  59. ncbi The tobacco aquaporin NtAQP1 is a membrane CO2 pore with physiological functions
    Norbert Uehlein
    Institute of Botany, Schnittspahnstrasse 3, D 64287 Darmstadt, Germany
    Nature 425:734-7. 2003
    ..The results indicate that NtAQP1-related CO2 permeability is of physiological importance under conditions where the CO2 gradient across a membrane is small, as is the case between the atmosphere and the inside of a plant cell...
  60. ncbi C-reactive protein makes human endothelium stiff and tight
    Kristina Kusche-Vihrog
    Institute of Physiology II, Muenster, Germany
    Hypertension 57:231-7. 2011
    ..The data indicate that CRP enhances the effects of aldosterone on the mechanical properties of the endothelium. Thus, CRP could counteract any decrease in arterial blood pressure that accompanies severe acute inflammatory processes...
  61. ncbi Sanglifehrin A acts as a potent inhibitor of the mitochondrial permeability transition and reperfusion injury of the heart by binding to cyclophilin-D at a different site from cyclosporin A
    Samantha J Clarke
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    J Biol Chem 277:34793-9. 2002
    ..Because SfA does not inhibit calcineurin activity, our data suggest that it may be more desirable than CsA for protecting tissues recovering from ischemic episodes and for studying the role of the MPTP in cell death...
  62. pmc Arginine-rich peptides destabilize the plasma membrane, consistent with a pore formation translocation mechanism of cell-penetrating peptides
    H D Herce
    Department of Physics, Rensselaer Polytechnic Institute, Troy, New York, USA
    Biophys J 97:1917-25. 2009
    ..Our results suggest that the mechanism of action of Arg-9 peptides involves the creation of transient pores in lipid bilayers and cell membranes...
  63. ncbi IFN-beta increases listeriolysin O-induced membrane permeabilization and death of macrophages
    Heather Zwaferink
    Max F Perutz Laboratories, Vienna Biocenter, Department of Microbiology and Immunobiology, University of Vienna, Vienna, Austria
    J Immunol 180:4116-23. 2008
    ..Key lipogenesis enzymes were suppressed in IFN-beta-treated cells, which may exacerbate the membrane damage caused by LLO...
  64. pmc Antimicrobial peptide-induced apoptotic death of leishmania results from calcium-de pend ent, caspase-independent mitochondrial toxicity
    Manjusha M Kulkarni
    Division of Infectious Diseases and Center for Microbial Interface Biology, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    J Biol Chem 284:15496-504. 2009
    ..Flufenamic acid, a nonspecific Ca2+ inhibitor, completely ablated AMP-induced mitochondrial dysfunction and cell death, indicating the importance of dysregulation of Ca2+ in antimicrobial peptide-induced apoptosis...
  65. ncbi Inhibition of nociceptors by TRPV1-mediated entry of impermeant sodium channel blockers
    Alexander M Binshtok
    Department of Anesthesia and Critical Care, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA
    Nature 449:607-10. 2007
  66. pmc Targeted epithelial tight junction dysfunction causes immune activation and contributes to development of experimental colitis
    Liping Su
    Department of Pathology, The University of Chicago, Chicago, Illinois 60637, USA
    Gastroenterology 136:551-63. 2009
    ..Our aim was to determine whether, in an intact epithelium, primary dysregulation of the intestinal epithelial barrier by pathophysiologically relevant mechanisms can contribute to development of colitis...
  67. ncbi RhoA, Rac1, and Cdc42 exert distinct effects on epithelial barrier via selective structural and biochemical modulation of junctional proteins and F-actin
    Matthias Bruewer
    Epithelial Pathobiology Research Unit, Department of Pathology and Lab Medicine, Emory University, Atlanta, GA 30322, USA
    Am J Physiol Cell Physiol 287:C327-35. 2004
  68. ncbi Membrane-permeable arginine-rich peptides and the translocation mechanisms
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan Presto, Japan Science and Technology Corporation JST, Kawaguchi, Saitama 332 0012, Japan
    Adv Drug Deliv Rev 57:547-58. 2005
    ..This review summarizes the structures of these peptide vectors, especially the Tat and other arginine-rich peptides, and the current understanding of their internalization mechanisms...
  69. ncbi Maintaining a healthy SPANC balance through regulatory and mutational adaptation
    Thomas Ferenci
    School of Molecular and Microbial Biosciences, The University of Sydney, NSW 2006, Australia
    Mol Microbiol 57:1-8. 2005
  70. pmc Resistance to caspase-independent cell death requires persistence of intact mitochondria
    Stephen W G Tait
    Department of Immunology, St Jude Children s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA
    Dev Cell 18:802-13. 2010
    ..Thus, iMOMP provides a critical source of intact mitochondria that permits cellular survival following MOMP...
  71. pmc Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O
    I Walev
    Institute of Medical Microbiology and Hygiene, Johannes Gutenberg University, 55101 Mainz, Germany
    Proc Natl Acad Sci U S A 98:3185-90. 2001
    ..botulinum C2-toxin resulted in enhanced secretion in cells. The presented method for introducing proteins into living cells should find multifaceted application in cell biology...
  72. ncbi Increased intestinal permeability to macromolecules and endotoxemia in patients with chronic alcohol abuse in different stages of alcohol-induced liver disease
    A Parlesak
    University of Hohenheim, Department of Physiology of Nutrition, Robert Bosch Krankenhaus, Stuttgart, Germany
    J Hepatol 32:742-7. 2000
  73. pmc The vitelliform macular dystrophy protein defines a new family of chloride channels
    Hui Sun
    Department of Molecular Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 99:4008-13. 2002
    ..These experiments establish the existence of a new chloride channel family and VMD as a channelopathy...
  74. pmc Na+ block and permeation in a K+ channel of known structure
    Crina M Nimigean
    Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454, USA
    J Gen Physiol 120:323-35. 2002
    ..The voltage dependence of Na(+) block reflects coordinated movements of the blocker with permeant ions in the selectivity filter...
  75. ncbi Digitonin permeabilization does not affect mitochondrial function and allows the determination of the mitochondrial membrane potential of Trypanosoma cruzi in situ
    A E Vercesi
    Department of Veterinary Pathobiology, University of Illinois, Urbana 61801
    J Biol Chem 266:14431-4. 1991
    ..The trypanocidal drug crystal violet, as well as other cationic drugs such as dequalinium, induced a rapid dose-related collapse of the inner mitochondrial membrane potential...
  76. ncbi Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein
    G M Preston
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205
    Science 256:385-7. 1992
    ..Therefore it is likely that CHIP28 is a functional unit of membrane water channels...
  77. ncbi Role of membranes in the activities of antimicrobial cationic peptides
    Robert E W Hancock
    Department of Microbiology and Immunology, University of British Columbia, 300 6174 University Blvd, Vancouver, BC, Canada V6T 1Z3
    FEMS Microbiol Lett 206:143-9. 2002
    ..Although dogma suggests that their lethal action involves disruption of the cytoplasmic membranes, a number of cationic peptides can traverse intact membranes to interact with internal targets...
  78. ncbi Transport of the essential nutrient isoleucine in human erythrocytes infected with the malaria parasite Plasmodium falciparum
    Rowena E Martin
    School of Biochemistry and Molecular Biology, The Australian National University, Canberra, Australia
    Blood 109:2217-24. 2007
    ..The accumulation of radiolabeled isoleucine within the parasite is mediated by a second (high-affinity, ATP-dependent) mechanism, perhaps involving metabolism and/or the concentration of isoleucine within an intracellular organelle...
  79. ncbi Mechanisms of cell membrane electropermeabilization: a minireview of our present (lack of ?) knowledge
    J Teissie
    IPBS UMR 5089 CNRS, 205 route de Narbonne, 31077 Toulouse, France
    Biochim Biophys Acta 1724:270-80. 2005
    ..Other possibilities are then given. The need for more basic research on the associated loss of cohesion of the membrane appears as a conclusion...
  80. ncbi Chitosan kills bacteria through cell membrane damage
    Hui Liu
    Department of Environmental Science, Wuhan University, Wuhan 430072, China
    Int J Food Microbiol 95:147-55. 2004
    ..This damage was likely caused by the electrostatic interaction between NH(3)(+) groups of CS acetate and phosphoryl groups of phospholipid components of cell membranes...
  81. ncbi Chitosan disrupts the barrier properties of the outer membrane of gram-negative bacteria
    I M Helander
    VTT Biotechnology and Food Research, Espoo, Finland
    Int J Food Microbiol 71:235-44. 2001
    ..Chitosan thus appeared to bind to the outer membrane, explaining the loss of the barrier function. This property makes chitosan a potentially useful indirect antimicrobial for food protection...
  82. ncbi Membrane injury by pore-forming proteins
    Mirko Bischofberger
    Ecole Polytechnique Federale de Lausanne, Global Health Institute, Lausanne, Switzerland
    Curr Opin Cell Biol 21:589-95. 2009
  83. pmc The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis
    Asaph Aharoni
    Plant Research International, 6700 AA, Wageningen, The Netherlands
    Plant Cell 16:2463-80. 2004
    ..We propose that these diverse functions are mediated by regulating metabolism of lipid and/or cell wall components...
  84. pmc Matrix metalloproteinase-9 knockout confers resistance to corneal epithelial barrier disruption in experimental dry eye
    Stephen C Pflugfelder
    Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Am J Pathol 166:61-71. 2005
    ..This appears to be because of accelerated loss of tight junction bearing superficial corneal epithelial cells, perhaps by proteolytic cleavage of occludin...
  85. ncbi Occludin as a possible determinant of tight junction permeability in endothelial cells
    T Hirase
    Eisai London Research Laboratories Ltd, University College London, UK
    J Cell Sci 110:1603-13. 1997
    ..Our data indicate that regulation of occludin expression may be a crucial determinant of the tight junction permeability properties of endothelial cells in different tissues...
  86. pmc Tight junctions and the modulation of barrier function in disease
    Carola Förster
    Institute of Anatomy and Cell Biology, University of Wurzburg, Koellikerstrasse 6, 97070, Wurzburg, Germany
    Histochem Cell Biol 130:55-70. 2008
    ..In many diseases, disruption of this regulated barrier occurs. This review will briefly describe the molecular composition of the tight junctions and then present evidence of the link between tight junction dysfunction and disease...
  87. ncbi A hydrophobic gate in an ion channel: the closed state of the nicotinic acetylcholine receptor
    Oliver Beckstein
    The Johns Hopkins University, School of Medicine, Department of Physiology, Biophysics 206, 725 N Wolfe St, Baltimore, MD 21205, USA
    Phys Biol 3:147-59. 2006
    ..Using a united-atom force field for the protein instead of an all-atom one retains the qualitative features but results in differing conductances, showing that the PMF is sensitive to the detailed molecular interactions...
  88. pmc Receptors of the protein C activation and activated protein C signaling pathways are colocalized in lipid rafts of endothelial cells
    Jong Sup Bae
    Edward A Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1402 South Grand Boulevard, St Louis, MO 63104, USA
    Proc Natl Acad Sci U S A 104:2867-72. 2007
  89. ncbi A two-parameter model of cell membrane permeability for multisolute systems
    I I Katkov
    Oak Ridge Associated Universities, Oak Ridge, Tennessee, 37831 0122, USA
    Cryobiology 40:64-83. 2000
    ..All formulas are simple to use for optimization of variety of cryobiological protocols. Application of the theory for optimization of addition and dilution of a permeable cryoprotectant is also discussed...
  90. pmc Enhancing the intestinal membrane permeability of zanamivir: a carrier mediated prodrug approach
    Sheeba Varghese Gupta
    Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, 428 Church Street, Ann Arbor, Michigan 48109, United States
    Mol Pharm 8:2358-67. 2011
  91. ncbi RhoA and NF-κB are involved in lipopolysaccharide-induced brain microvascular cell line hyperpermeability
    F He
    Department of Pediatrics, Xiangya Hospital, Central South University, Changsha, Hunan 41008, PR China
    Neuroscience 188:35-47. 2011
    ..The aim of this study was to investigate the signaling mechanisms surrounding changes in tight junction (TJ) and the permeability of brain microvascular cell lines induced by lipopolysaccharide (LPS)...
  92. ncbi The membrane action mechanism of analogs of the antimicrobial peptide Buforin 2
    Gang Hao
    The State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu Province, China
    Peptides 30:1421-7. 2009
    ..The leakage of cytoplasmic contents was also demonstrated by the electron micrographs. The increase of membrane permeabilization explained why BF2-B displayed better antimicrobial activity and rapid killing kinetics than BF2-A...
  93. ncbi Delivery of proteins and nucleic acids using a non-covalent peptide-based strategy
    Sebastien Deshayes
    Centre de Recherches de Biochimie Macromoleculaire, CRBM CNRS, UMR5237, Department of Molecular Biophysics and Therapeutics, 1919 Route de Mende, 34293 Montpellier, France
    Adv Drug Deliv Rev 60:537-47. 2008
    ..This review will focus on the mechanisms of non-covalent MPG and Pep-1 strategies and their applications in cultured cells and animal models...
  94. ncbi Red but not dead? Membranes of stressed Saccharomyces cerevisiae are permeable to propidium iodide
    H M Davey
    Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Penglais, Aberystwyth, UK
    Environ Microbiol 13:163-71. 2011
    ..We discuss these findings in the context of the potential for such environmental stress-induced, transient membrane permeability to have evolutionary implications via the facilitation of horizontal gene transfer...
  95. pmc Bacillus thuringiensis Cry1Ac toxin-binding and pore-forming activity in brush border membrane vesicles prepared from anterior and posterior midgut regions of lepidopteran larvae
    Ana Rodrigo-Simón
    Departamento de Genetica, Facultad de CC Biológicas, Dr Moliner 50, 46100 Burjassot Valencia, Spain
    Appl Environ Microbiol 74:1710-6. 2008
    ..The analysis of binding and pore formation in different midgut regions could be an effective method to study differences in the mode of action of Cry1Ac toxin in different species...
  96. ncbi Characterization of Glossy1-homologous genes in rice involved in leaf wax accumulation and drought resistance
    Mohammad Asadul Islam
    National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research Wuhan, Huazhong Agricultural University, Wuhan, China
    Plant Mol Biol 70:443-56. 2009
    ..Compared to the over-expression and wild type plants, the osgl1-2 mutant was more sensitive to drought stress at reproductive stage, suggesting an important role of this gene in drought resistance...
  97. ncbi Sevenfold-reduced osmotic water permeability in primary astrocyte cultures from AQP-4-deficient mice, measured by a fluorescence quenching method
    Eugen Solenov
    Department of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143 0521, USA
    Am J Physiol Cell Physiol 286:C426-32. 2004
    ..3 +/- 0.5 vs. 5.5 +/- 0.4 kcal/mol). Our studies establish a calcein quenching method for measurement of cell membrane water permeability and indicate that AQP-4 provides the principal route for water transport in astrocytes...
  98. ncbi Roles of ZO-1 and ZO-2 in establishment of the belt-like adherens and tight junctions with paracellular permselective barrier function
    Sachiko Tsukita
    Laboratory of Biological Science, Graduate School of Frontier Biosciences and Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
    Ann N Y Acad Sci 1165:44-52. 2009
  99. ncbi BH3 death domain peptide induces cell type-selective mitochondrial outer membrane permeability
    B M Polster
    Department of Anesthesiology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 276:37887-94. 2001
    ..Overall, results support the hypothesis that BH3 peptide releases cytochrome c by a Bax-dependent process that is independent of the mitochondrial permeability transition pore but regulated by Bcl-2...
  100. ncbi Shear stress in cells generated by ultrasound
    Junru Wu
    Department of Physics, University of Vermont, Burlington, VT 05405, USA
    Prog Biophys Mol Biol 93:363-73. 2007
    ..Numerical calculations have shown that shear stress associated with microstreaming surrounding encapsulated bubbles may be large enough to generate sonoporation at 0.1 MPa of 1 or 2 MHz ultrasound...
  101. ncbi Intracellular pH sensing is altered by plasma membrane PIP aquaporin co-expression
    Jorge Bellati
    Departamento de Fisiologia, Universidad de Buenos Aires, Argentina
    Plant Mol Biol 74:105-18. 2010
    ..These results prove for the first time that PIP co-expression modulates the membrane water permeability through a pH regulatory response, enhancing in this way membrane versatility to adjust its water transfer capacity...

Research Grants66

  1. Toxicity Studies of Potent Antisickling Agent 5HMF
    Robert Swift; Fiscal Year: 2006
    ..studies with Tg sickle mice show that 5HMF is orally active with high bioavailability and has high red cell membrane permeability. 5HMF prolongs the life of severe hypoxic transgenic sickle cell (Tg) mice significantly and inhibits ..
  2. Ultrasound-activated microbubbles for targeted siRNA delivery to tumor
    Flordeliza S Villanueva; Fiscal Year: 2013
    ..These bioeffects include enhanced cell membrane permeability to macromolecules, which is thought to facilitate siRNA internalization...
    Mai Xu; Fiscal Year: 2013
    ..Note that hyperthermia has already been used to improve blood circulation, increase tumor cell membrane permeability for anticancer chemical diffusion passively, oxygenate hypoxia tumor cells, and inhibit DNA repair to ..
  4. Targeted theranostic microbubble vectors for transcription factor decoy delivery
    Flordeliza S Villanueva; Fiscal Year: 2013
    ..These bioeffects include enhanced cell membrane permeability to macromolecules, such as transcription factor decoys, which can be loaded on MBs and released solely ..
  5. Allosteric Inhibition of a Family of Proteolytic Enzymes
    CHARLES SCOTT CRAIK; Fiscal Year: 2013
    ..proteases using screening and structure-guided chemistry to achieve nanomolar inhibition and improved cell membrane permeability. Aim 2...
  6. A Novel Target for New Anti-Anthrax Drugs
    Wayne Brouillette; Fiscal Year: 2007
    ..Since exogenous NAD cannot support bacterial growth due to insufficient cell membrane permeability/transport, inhibitors of NAD biosynthesis should be bacteriostatic and/or bacteriocidal...
  7. Ultrasound contrast agent dynamics and vascular effects
    THOMAS MATULA; Fiscal Year: 2009
    ..targeted drug delivery or to enhance gene therapy, where changes in the vascular endothelium or cell membrane permeability are thought to be critical...
    CHRISTOPHER PATERSON; Fiscal Year: 2004
    ..Calcium homeostasis is made possible through a delicate balance between lens cell membrane permeability and the energy dependent transport of calcium by Ca-ATPase at the level of the lens cell membrane (PM Ca-..
    Kullervo Hynynen; Fiscal Year: 2002
    ..deep tissue, close blood vessels, activate drugs, open the blood brain barrier, and perhaps increase the cell membrane permeability to molecules...
  10. Aptamer Screening for Bioterrorism Agent Therapeutics
    Manfred Koller; Fiscal Year: 2002
    ..g. plasmids. proteins, etc.), whereby a laser is used to increase the cell membrane permeability, allowing the substance to enter the cell...
    Robert Bernard; Fiscal Year: 2000
    ..By applying brief electrical pulses to tissue, transient increases in cell membrane permeability can be induced...
  12. Cell Membrane Simulators in Biocompatibility Testing
    DAVID YOURTEE; Fiscal Year: 2006
    ..membranes through the study of the thermodynamics of BioSims; 3) improve knowledge of dental monomer cell membrane permeability by determining their effect on the permeability of BioSims; 4) establish effects of biomaterials on ..
    LARRY MC INTIRE; Fiscal Year: 1992
    ..epi-fluorescent video microscopy and image analysis techniques; and (2) effect of shear stress on red cell membrane permeability and the sickle hemoglobin polymerization rate and final gel material properties...
  14. Thiazolidinedione-induced Hepatotoxicity
    PETER HARVISON; Fiscal Year: 2009
    ..will be used to in an effort to discriminate between necrotic and apoptotic cell death pathways: (a) cell membrane permeability, (b) cellular ATP content, (c) nuclear morphology and (d) staining with annexin V-FITC/propidium iodide...
  15. Fluorosome Technique for Drug Permeability Studies
    George Wright; Fiscal Year: 2005
    ..In phase II we will develop a product that will be predictive of intestinal cell membrane permeability and of the oral absorption of drugs...
  16. Expression, function and regulation of renal claudins
    ALAN S YU; Fiscal Year: 2010
    ..This may shed light on disorders such as hypertension and renal salt-wasting and lead to the development of drugs that could regulate paracellular permeability of the renal tubule and correct electrolyte disorders. ..
    Andrew Harris; Fiscal Year: 2006
    ..The information obtained will be informative regarding connexin channel function in other contexts, and will lead to insights about mechanisms of selectivity among cytoplasmic permeants. ..
  18. Colon Barrier Breakdown in the Elderly
    James Mullin; Fiscal Year: 2005
    ..Changes in membrane composition/fluidity are known to affect paracellular permeability in general, and tight junction proteins specifically. ..
    Andrew Harris; Fiscal Year: 2009
    ..In the present proposal, this analysis will be applied to Cx32 and Cx26, defects in which cause X-linked Charcot-Marie-Tooth disease neuropathy and sensorineural deafness, respectively. ..
  20. Purchase of a 500 MHz Solid State NMR Spectrometer
    Ayyalusamy Ramamoorthy; Fiscal Year: 2007
    ..unreadable] [unreadable] All the major users of the instrument are supported by NIH R01 grants. [unreadable] [unreadable] [unreadable]..
  21. Estrogen and Aging effects on Transvaginal Transport
    George Gorodeski; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
    Andrew Harris; Fiscal Year: 2007
    ..The information obtained will inform cellular and physiological studies of intercellular communication in disease and development. ..
  23. Structure and function of antimicrobial peptides
    Ayyalusamy Ramamoorthy; Fiscal Year: 2008
    ..These peptides have therapeutic potential as antibiotics and have particular importance for cystic fibrosis patients. ..
  24. Stem Cell-Seeded ECM Scaffolds for Neurotransplantation
    MICHELLE LAPLACA; Fiscal Year: 2004
    ..abstract_text> ..
    Andrew Harris; Fiscal Year: 2002
    ..Such defects will result in abnormal (i.e., greater or lesser) intercellular signaling molecules. The proposed studies address how this may occur. ..
    Mary Hagedorn; Fiscal Year: 2005
    ..Most importantly, these somatic changes will not affect the germ line, so they are ideal for genomic banking to maintain important genetic stocks for biomedical and endangered fish research. ..
  27. Identification of Biomarkers of Autoimmunity in T1D by Novel Tools
    Mikael Knip; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  28. NO production and S-nitrosylation in liver vasculature
    Yasuko Iwakiri; Fiscal Year: 2008
    ..The School of Medicine has pledged protected time for the candidate during this further training period prior to functioning as an independent investigator. ..
  29. Characterization of cardiovascular purinoceptors
    TERRANCE EGAN; Fiscal Year: 2009
    ..This is important because the receptors may present a new and potentially exciting means of increasing the rate of survival of patients affected by a number of cardiovascular diseases including hypertension and heart failure. ..
    Douglas Miller; Fiscal Year: 2003
    ..This research will provide a bridge from the basic research breakthrough to specific therapeutic applications and urgently needed clinical research programs utilizing this novel technology. ..
  31. Training Program in Molecular and Cellular Toxicology
    John Reiners; Fiscal Year: 2008
    ..It is envisioned that students with such training will be better equipped to pursue postdoctoral research at major research laboratories throughout the United States. ..
    Barbara Miller; Fiscal Year: 2007
    ..abstract_text> ..
    MICHAEL FLESSNER; Fiscal Year: 2004
  34. Dual Confocal Microscopic Scanner
    Eduardo Rios; Fiscal Year: 2008
    ..The extraordinary level of institutional support bestowed on this proposal attests to its perceived importance for basic and translational research at Rush University. [unreadable] [unreadable] [unreadable]..
  35. Conference on Mitochondria and Pathogenesis
    John Lemasters; Fiscal Year: 2002
    ..The purpose of this conference is to bring together leading researchers studying mitochondrial dysfunction in many different disease settings to share ideas and recent research findings. ..
  36. 2008 Bacterial Cell Surfaces Gordon Research Conference
    ANNE DELCOUR; Fiscal Year: 2008
    ..Success in these research endeavors is enhanced by the exchange of information and ideas promoted by the Bacterial Cell Surfaces Gordon Conference. [unreadable] [unreadable] [unreadable]..
  37. Mg2+ homeostasis in liver cells treated with EtOH
    Andrea Romani; Fiscal Year: 2009
  38. Initiation of Cell Death after Traumatic Brain Injury
    Michael Whalen; Fiscal Year: 2009
    ..The goal of this revised proposal is to conclusively demonstrate that redundant and opposing effects of multiple TNFR family members integrate to significantly influence cell death, as well as motor and cognitive outcome, after TBI. ..
    Marcel Bruchez; Fiscal Year: 2002
    ..The technology is also directly applicable to many different kinds of tumor analysis and could be used on tissue microarrays to vastly increase the throughput and aid the archiving of tissue section analysis. ..
  40. Bladder-specific Knockout and Gene Expression
    Xue ru Wu; Fiscal Year: 2009
    ..These studies will generate powerful experimental tools for studying bladder biology and diseases and offer molecular insights regarding urothelial growth and tumorigenesis. ..
  41. Role of BCL-xL in synaptic plasticity in the hippocampus
    Elizabeth Ann Jonas; Fiscal Year: 2010
    ..We hypothesize that these changes are required for long term changes in synaptic strength after high frequency synaptic stimulation, therefore we plan to test whether inhibition of BClxl prevents l TP. ..
    Stephen Thom; Fiscal Year: 2003
    ..The research plan is aimed to test the hypothesis that immune responses and the associated oxidative stress cause neurological injuries. ..
  43. Effects of HAART drugs on endothelial dysfunction of pu*
    Changyi Chen; Fiscal Year: 2009
    ..abstract_text> ..
    James Tidball; Fiscal Year: 2001
    ..Hind limb immobilization at shortened length followed by remobilization will be used to examine the effect of inhibition of NOS on sarcomere addition. ..
  45. Ice Nucelation in Biological Tissues - Implications to Cryopreservation
    Ram Devireddy; Fiscal Year: 2007
    ..SA2: Extension of the technique to measure ice nucleation kinetics in whole tissues. SA3: Modeling and prediction of ice nucleation kinetics in whole tissue sections. [unreadable] [unreadable] [unreadable]..
  46. Measurement of Fluid Flow Across the Ocular Ciliary Body
    OSCAR CANDIA; Fiscal Year: 2006
    ..abstract_text> ..
  47. Restraint stress-induced neurogenic bladder inflammation
    Theoharis Theoharides; Fiscal Year: 2006
    ..Our findings may be relevant to the pathophysiology of IC and may suggest new therapeutic approaches. ..
    LAWRENCE RIZZOLO; Fiscal Year: 2006
    ..Recent advances in the development of reagents coupled with this novel culture model will advance our understanding of this essential structure of the outer blood-retinal barrier. [unreadable] [unreadable]..
  49. Role of Regulated CIC CI Channels in CI Secretion in CF
    John Cuppoletti; Fiscal Year: 2006
    ..2 K channel in CF. This will also be studied in terms of CI transport, Na transport, CLCA2 function, inflammation and bacterial susceptibility. ..
  50. Molecular Pathogenesis of Apoptosis in Liver Cells
    John Lemasters; Fiscal Year: 2005
    ..abstract_text> ..
  51. Imaging of O6-Alkylguanine-DNA Alkyltransferase
    Ganesan Vaidyanathan; Fiscal Year: 2005
    ..In summary, this proposal seeks to determine whether the development of a suitable agent for the imaging of tumor AGT will facilitate chemotherapy planning, thereby improving the outcome of alkylator chemotherapy. ..
  52. Conference on Cellular Senescene and Cell Death
    Douglas Green; Fiscal Year: 2005
    ..The interconnections between these biological events and the overlapping principles that emerge represent an emerging field in biology and biomedical research. ..
  53. The Glycocalyx and Endothelial Barrier Regulation
    RANDAL DULL; Fiscal Year: 2005
    ..This K08 will provide the foundation for a research career dedicated to understanding the molecular mechanisms of endothelial barrier regulation. ..
  54. Two-Photon Microscope Patch Clamp Integrated System
    Meyer Jackson; Fiscal Year: 2005
    ..abstract_text> ..
  55. Cochlear Type II Afferents and Nociception in the Inner*
    Jaime Garcia Anoveros; Fiscal Year: 2004
  56. HIV-1 gp120-induced Endothelial Cell Dysfunction
    GEORGETTE KANMOGNE; Fiscal Year: 2007
    Changyi Chen; Fiscal Year: 2002
    PAUL AXELSEN; Fiscal Year: 2004
    ..The overall aim is to confront the emerging health threat of vancomycin-resistance by facilitating the rational design of drugs, and gain insight into the physico-chemical basis of specific molecular recognition. ..
  59. Mast cells, antidepressants and chronic fatigue syndrome
    Theoharis Theoharides; Fiscal Year: 2007
    ..Future studies will build on these findings to develop in vitro and in vivo models of CFS and lead to clinical trials with select antidepressants or other molecules that inhibit brain mast cells. [unreadable] [unreadable] [unreadable]..
  60. Focusing oral health research at the University of Iowa
    Christopher Squier; Fiscal Year: 2003
    ..abstract_text> ..
    HAROLD DVORAK; Fiscal Year: 2008
    ..Aim 2. Elucidate the mechanisms by which mother vessels evolve into daughter vessels. Aim 3. Generate normal blood vessels with mixtures of primary and secondary cytokines. ..
  62. Molecular and Cellular Analyses of Aquaporins in Lung
    LANDON KING; Fiscal Year: 2005
    ..These studies should provide insight into mechanisms regulating expression of AQP1 and AQP5, and establish a framework from which to better understand their role in the pathophysiology of the respiratory tract. ..
    Seth Alper; Fiscal Year: 2007
    ..5. Define aspects of transcriptional and translational regulation of AE gene products in kidney of mutant and parental mouse strains and in cultured kidney cells. ..
  64. Porous Silicon Particles for Oral Drug Delivery
    Peter Swaan; Fiscal Year: 2007
    ..This may allow for the delivery of multiple therapeutic macromolecules in a more controlled and targeted manner to the GI tract. [unreadable] [unreadable]..
  65. Sickle Red Cell K+ Transporter Genetics in S. cerevisiae
    Seth Alper; Fiscal Year: 2002
    ..3) (Provisional Aim): Time permitting, to improve, validate, and standardize growth rescue of trk1delta/trk2delta S. cerevisiae by expression in nonpermissive conditions of cDNA encoding the mammalian IK1 K(ca) channel. ..