cell lineage

Summary

Summary: The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the EMBRYO.

Top Publications

  1. ncbi Multilineage potential of adult human mesenchymal stem cells
    M F Pittenger
    Osiris Therapeutics, 2001 Aliceanna Street, Baltimore, MD 21231 3043, USA
    Science 284:143-7. 1999
  2. ncbi Matrix elasticity directs stem cell lineage specification
    Adam J Engler
    Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, 19104, USA
    Cell 126:677-89. 2006
  3. ncbi Multilineage cells from human adipose tissue: implications for cell-based therapies
    P A Zuk
    Laboratory for Regenerative Bioengineering and Repair, UCLA School of Medicine, Los Angeles, California, USA
    Tissue Eng 7:211-28. 2001
  4. ncbi Pluripotency of mesenchymal stem cells derived from adult marrow
    Yuehua Jiang
    Stem Cell Institute, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Nature 418:41-9. 2002
  5. doi Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells
    Hugo J Snippert
    Hubrecht Institute, KNAW and University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Cell 143:134-44. 2010
  6. pmc Direct conversion of fibroblasts to functional neurons by defined factors
    Thomas Vierbuchen
    Institute for Stem Cell Biology and Regenerative Medicine, Department of Pathology, Stanford University School of Medicine, 1050 Arastradero Road, Palo Alto, California 94304, USA
    Nature 463:1035-41. 2010
  7. pmc The glial nature of embryonic and adult neural stem cells
    Arnold Kriegstein
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Department of Neurology, University of California, San Francisco, California 94143 0525, USA
    Annu Rev Neurosci 32:149-84. 2009
  8. ncbi BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3
    Qi Long Ying
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, EH9 3JQ, Edinburgh, Scotland
    Cell 115:281-92. 2003
  9. ncbi Adult cardiac stem cells are multipotent and support myocardial regeneration
    Antonio P Beltrami
    Cardiovascular Research Institute, Department of Medicine, New York Medical College, Valhalla, NY 10595, USA
    Cell 114:763-76. 2003
  10. pmc Modelling pathogenesis and treatment of familial dysautonomia using patient-specific iPSCs
    Gabsang Lee
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, USA
    Nature 461:402-6. 2009

Detail Information

Publications327 found, 100 shown here

  1. ncbi Multilineage potential of adult human mesenchymal stem cells
    M F Pittenger
    Osiris Therapeutics, 2001 Aliceanna Street, Baltimore, MD 21231 3043, USA
    Science 284:143-7. 1999
    ..These adult stem cells could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages. Individual stem cells were identified that, when expanded to colonies, retained their multilineage potential...
  2. ncbi Matrix elasticity directs stem cell lineage specification
    Adam J Engler
    Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, 19104, USA
    Cell 126:677-89. 2006
    Microenvironments appear important in stem cell lineage specification but can be difficult to adequately characterize or control with soft tissues...
  3. ncbi Multilineage cells from human adipose tissue: implications for cell-based therapies
    P A Zuk
    Laboratory for Regenerative Bioengineering and Repair, UCLA School of Medicine, Los Angeles, California, USA
    Tissue Eng 7:211-28. 2001
    ..In conclusion, the data support the hypothesis that a human lipoaspirate contains multipotent cells and may represent an alternative stem cell source to bone marrow-derived MSCs...
  4. ncbi Pluripotency of mesenchymal stem cells derived from adult marrow
    Yuehua Jiang
    Stem Cell Institute, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Nature 418:41-9. 2002
    ..As MAPCs proliferate extensively without obvious senescence or loss of differentiation potential, they may be an ideal cell source for therapy of inherited or degenerative diseases...
  5. doi Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells
    Hugo J Snippert
    Hubrecht Institute, KNAW and University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Cell 143:134-44. 2010
    ..Quantitative analysis shows that stem cell turnover follows a pattern of neutral drift dynamics...
  6. pmc Direct conversion of fibroblasts to functional neurons by defined factors
    Thomas Vierbuchen
    Institute for Stem Cell Biology and Regenerative Medicine, Department of Pathology, Stanford University School of Medicine, 1050 Arastradero Road, Palo Alto, California 94304, USA
    Nature 463:1035-41. 2010
    ..Generation of iN cells from non-neural lineages could have important implications for studies of neural development, neurological disease modelling and regenerative medicine...
  7. pmc The glial nature of embryonic and adult neural stem cells
    Arnold Kriegstein
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Department of Neurology, University of California, San Francisco, California 94143 0525, USA
    Annu Rev Neurosci 32:149-84. 2009
    ..Identification of NSCs and IPCs is critical to understand brain development and adult neurogenesis and to develop new strategies for brain repair...
  8. ncbi BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3
    Qi Long Ying
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, EH9 3JQ, Edinburgh, Scotland
    Cell 115:281-92. 2003
    ..Upon LIF withdrawal, Id-expressing ES cells differentiate but do not give rise to neural lineages. We conclude that blockade of lineage-specific transcription factors by Id proteins enables the self-renewal response to LIF/STAT3...
  9. ncbi Adult cardiac stem cells are multipotent and support myocardial regeneration
    Antonio P Beltrami
    Cardiovascular Research Institute, Department of Medicine, New York Medical College, Valhalla, NY 10595, USA
    Cell 114:763-76. 2003
    ..The existence of these cells opens new opportunities for myocardial repair...
  10. pmc Modelling pathogenesis and treatment of familial dysautonomia using patient-specific iPSCs
    Gabsang Lee
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, USA
    Nature 461:402-6. 2009
    ..Our study illustrates the promise of iPSC technology for gaining new insights into human disease pathogenesis and treatment...
  11. pmc Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
    Tarjei S Mikkelsen
    Broad Institute of Harvard and MIT, Cambridge, Massachusetts 02142, USA
    Nature 448:553-60. 2007
    ..This study provides a framework for the application of comprehensive chromatin profiling towards characterization of diverse mammalian cell populations...
  12. pmc Human adipose tissue is a source of multipotent stem cells
    Patricia A Zuk
    Department of Surgery and Orthopedics, Regenerative Bioengineering and Repair Laboratory, UCLA School of Medicine, Los Angeles, California 90095, USA
    Mol Biol Cell 13:4279-95. 2002
    ..Finally, PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression...
  13. pmc Coexistence of quiescent and active adult stem cells in mammals
    Linheng Li
    Stowers Institute for Medical Research SIMR, Kansas City, MO 64110, USA
    Science 327:542-5. 2010
    ..Here, we summarize these findings and propose that quiescent and active stem cell populations have separate but cooperative functional roles...
  14. pmc Regulated fluctuations in nanog expression mediate cell fate decisions in embryonic stem cells
    Tibor Kalmar
    Department of Genetics, University of Cambridge, Cambridge, United Kingdom
    PLoS Biol 7:e1000149. 2009
    ....
  15. pmc Dissecting direct reprogramming through integrative genomic analysis
    Tarjei S Mikkelsen
    Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 454:49-55. 2008
    ..We demonstrate that RNA inhibition of transcription factors can facilitate reprogramming, and that treatment with DNA methyltransferase inhibitors can improve the overall efficiency of the reprogramming process...
  16. pmc Cell type of origin influences the molecular and functional properties of mouse induced pluripotent stem cells
    Jose M Polo
    Howard Hughes Medical Institute and Department of Stem Cell and Regenerative Biology, Harvard University and Harvard Medical School, Cambridge, Massachusetts, USA
    Nat Biotechnol 28:848-55. 2010
    ..These observations may influence ongoing attempts to use iPSCs for disease modeling and could also be exploited in potential therapeutic applications to enhance differentiation into desired cell lineages...
  17. ncbi Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment
    Rowena McBeath
    The Cellular and Molecular Medicine Program, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Dev Cell 6:483-95. 2004
    ..These studies demonstrate that mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate...
  18. ncbi MicroRNAs modulate hematopoietic lineage differentiation
    Chang Zheng Chen
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
    Science 303:83-6. 2004
    ..Our results indicate that microRNAs are components of the molecular circuitry that controls mouse hematopoiesis and suggest that other microRNAs have similar regulatory roles during other facets of vertebrate development...
  19. ncbi A clonogenic common myeloid progenitor that gives rise to all myeloid lineages
    K Akashi
    Department of Pathology, Stanford University School of Medicine, California 94305, USA
    Nature 404:193-7. 2000
    ....
  20. pmc Stability of the regulatory T cell lineage in vivo
    Yuri P Rubtsov
    Howard Hughes Medical Institute and Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Science 329:1667-71. 2010
    ..Our results suggest that self-renewal of mature T(reg) cells serves as a major mechanism of maintenance of the T(reg) cell lineage in adult mice.
  21. pmc Epigenetic memory in induced pluripotent stem cells
    K Kim
    Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 467:285-90. 2010
    ....
  22. doi Plasticity of CD4+ T cell lineage differentiation
    Liang Zhou
    The Kimmel Center for Biology and Medicine at the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA
    Immunity 30:646-55. 2009
    ..Here, we will discuss recent findings that demonstrate the plasticity of CD4(+) T cell differentiation and the biological implications of this flexibility...
  23. ncbi In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
    Marius Wernig
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nature 448:318-24. 2007
    ..Our results show that the biological potency and epigenetic state of in-vitro-reprogrammed induced pluripotent stem cells are indistinguishable from those of ES cells...
  24. ncbi Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology CDB, 2 2 3 Minatojima minamimachi, Kobe, Hyogo 650 0047, Japan
    Cell 123:917-29. 2005
    Trophectoderm (TE), the first differentiated cell lineage of mammalian embryogenesis, forms the placenta, a structure unique to mammalian development...
  25. pmc The dendritic cell lineage: ontogeny and function of dendritic cells and their subsets in the steady state and the inflamed setting
    Miriam Merad
    Department of Oncological Sciences, Mount Sinai Medical School, New York, NY 10029, USA
    Annu Rev Immunol 31:563-604. 2013
    ..This review discusses major advances in our understanding of the regulation of DC lineage commitment, differentiation, diversification, and function in situ...
  26. doi Strategies for homeostatic stem cell self-renewal in adult tissues
    Benjamin D Simons
    Cavendish Laboratory, Department of Physics, J J Thomson Avenue, University of Cambridge, Cambridge CB3 0HE, United Kingdom
    Cell 145:851-62. 2011
    ..Here, we consider the general strategies for stem cell self-renewal and review the evidence for stochastic stem cell fate in adult tissues across a range of tissue types and organisms...
  27. doi Forcing cells to change lineages
    Thomas Graf
    Center for Genomic Regulation and ICREA, 08003 Barcelona, Spain
    Nature 462:587-94. 2009
    ..The lessons learnt provide a rubric for how cells may be manipulated for therapeutic purposes...
  28. ncbi A single type of progenitor cell maintains normal epidermis
    Elizabeth Clayton
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Cambridge CB2 0XZ, UK
    Nature 446:185-9. 2007
    ..These cells are found to undergo both symmetric and asymmetric division at rates that ensure epidermal homeostasis. The results raise important questions about the potential role of stem cells on tissue maintenance in vivo...
  29. ncbi Isolation of multipotent mesenchymal stem cells from umbilical cord blood
    Oscar K Lee
    Department of Orthopaedics and Traumatology, Veterans General Hospital Taipei, Taipei 11221, Taiwan
    Blood 103:1669-75. 2004
    ..In conclusion, umbilical cord blood does contain mesenchymal stem cells and should not be regarded as medical waste. It can serve as an alternative source of mesenchymal stem cells to bone marrow...
  30. doi Crypt stem cells as the cells-of-origin of intestinal cancer
    Nick Barker
    Hubrecht Institute for Developmental Biology and Stem Cell Research, Uppsalalaan 8, 3584CT Utrecht and University Medical Centre Utrecht, Netherlands
    Nature 457:608-11. 2009
    ..Even after 30weeks, large adenomas are very rare in these mice. We conclude that stem-cell-specific loss of Apc results in progressively growing neoplasia...
  31. pmc In vivo analysis of dendritic cell development and homeostasis
    Kang Liu
    Laboratory of Molecular Immunology, Rockefeller University, New York, NY 10065, USA
    Science 324:392-7. 2009
    ..Further cDC development involves cell division, which is controlled in part by regulatory T cells and fms-like tyrosine kinase receptor-3...
  32. pmc Conversion of adult pancreatic alpha-cells to beta-cells after extreme beta-cell loss
    Fabrizio Thorel
    Department of Cell Physiology and Metabolism, University of Geneva Faculty of Medicine, 1 rue Michel Servet, CH 1211 Geneva 4, Switzerland
    Nature 464:1149-54. 2010
    ..Such inter-endocrine spontaneous adult cell conversion could be harnessed towards methods of producing beta-cells for diabetes therapies, either in differentiation settings in vitro or in induced regeneration...
  33. pmc Chromatin signatures in multipotent human hematopoietic stem cells indicate the fate of bivalent genes during differentiation
    Kairong Cui
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Cell Stem Cell 4:80-93. 2009
    ..Thus, our data suggest that gene expression changes during differentiation are programmed by chromatin modifications present at the HSC/HPC stage and provide a resource for enhancer and promoter identification...
  34. pmc Deciphering the transcriptional network of the dendritic cell lineage
    Jennifer C Miller
    Immunology Institute, Mount Sinai School of Medicine, New York, New York, USA
    Nat Immunol 13:888-99. 2012
    ..We also identified a transcriptional program expressed specifically during the steady-state migration of tissue DCs to the draining lymph nodes that may control tolerance to self tissue antigens...
  35. ncbi Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway
    Claire Chazaud
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G1X5, Canada
    Dev Cell 10:615-24. 2006
    ..We propose a model in which the ICM develops as a mosaic of EPI and PE progenitors at E3.5, dependent on Grb2-Ras-MAP kinase signaling, followed by later segregation of the progenitors into the appropriate cell layers...
  36. ncbi Isl1 identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of cells to the heart
    Chen Leng Cai
    Institute of Molecular Medicine, Department of Medicine, University of California San Diego, La Jolla, CA 92093, USA
    Dev Cell 5:877-89. 2003
    ..Our results have implications for the development of specific cardiac lineages, left-right asymmetry, cardiac evolution, and isolation of cardiac progenitor cells...
  37. ncbi Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells
    H Niwa
    Centre for Genome Research, The University of Edinburgh, King s Buildings, Edinburgh, UK
    Nat Genet 24:372-6. 2000
    ....
  38. ncbi In vitro differentiation of transplantable neural precursors from human embryonic stem cells
    S C Zhang
    Department of Anatomy, University of Wisconsin 1500 Highland Avenue, Madison, WI 53705, USA
    Nat Biotechnol 19:1129-33. 2001
    ..No teratoma formation was observed in the transplant recipients. These results depict human ES cells as a source of transplantable neural precursors for possible nervous system repair...
  39. ncbi Postnatal isl1+ cardioblasts enter fully differentiated cardiomyocyte lineages
    Karl Ludwig Laugwitz
    Institute of Molecular Medicine, University of California, San Diego, School of Medicine, La Jolla, California 92093, USA
    Nature 433:647-53. 2005
    ..of native cardiac progenitors would form a mechanistic underpinning for unravelling steps for cardiac cell lineage formation, and their links to forms of congenital and adult cardiac diseases...
  40. doi Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population
    Lei Yang
    Department of Gene and Cell Medicine, The Black Family Stem Cell Institute, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, New York 10029, USA
    Nature 453:524-8. 2008
    ..Together, these findings identify a human cardiovascular progenitor that defines one of the earliest stages of human cardiac development...
  41. pmc Distinct epigenomic landscapes of pluripotent and lineage-committed human cells
    R David Hawkins
    Ludwig Institute for Cancer Research, La Jolla, CA 92093, USA
    Cell Stem Cell 6:479-91. 2010
    ..Our results provide new insights into epigenetic mechanisms underlying properties of pluripotency and cell fate commitment...
  42. pmc Global mapping of H3K4me3 and H3K27me3 reveals specificity and plasticity in lineage fate determination of differentiating CD4+ T cells
    Gang Wei
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Immunity 30:155-67. 2009
    ..Our data suggest an epigenetic mechanism underlying the specificity and plasticity of effector and regulatory T cells and also provide a framework for understanding complexity of CD4(+) T helper cell differentiation...
  43. ncbi A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration
    Thorsten Buch
    Laboratory for Molecular Immunology, Institute for Genetics, University of Cologne, D 50931 Cologne, Germany
    Nat Methods 2:419-26. 2005
    ..Notably, we show that the developing DT-specific antibody response is weak and not neutralizing, and thus does not impede the efficacy of DT. Our results validate the use of iDTR mice as a tool for cell ablation in vivo...
  44. ncbi Out of Eden: stem cells and their niches
    F M Watt
    Keratinocyte Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Science 287:1427-30. 2000
    ..Certain aspects of the stem cell microenvironment, or niche, are conserved between tissues, and this can be exploited in the application of stem cells to tissue replacement therapy...
  45. pmc Tet1 and Tet2 regulate 5-hydroxymethylcytosine production and cell lineage specification in mouse embryonic stem cells
    Kian Peng Koh
    Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital Boston, Boston, MA 02115, USA
    Cell Stem Cell 8:200-13. 2011
    ..In Fgf4- and heparin-supplemented culture conditions, Tet1-depleted ESCs activate the trophoblast stem cell lineage determinant Elf5 and can colonize the placenta in midgestation embryo chimeras...
  46. doi Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst
    Guoji Guo
    Department of Biological Sciences, National University of Singapore, Singapore 117543
    Dev Cell 18:675-85. 2010
    ..These results illustrate the power of single-cell expression analysis to provide insight into developmental mechanisms. The technique should be widely applicable to other biological systems...
  47. doi Intestinal label-retaining cells are secretory precursors expressing Lgr5
    Simon J A Buczacki
    Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Nature 495:65-9. 2013
    ..These findings establish quiescent cells as an effective clonogenic reserve and provide a motivation for investigating their role in pathologies such as colorectal cancers and intestinal inflammation...
  48. pmc Dynamic equilibrium and heterogeneity of mouse pluripotent stem cells with distinct functional and epigenetic states
    Katsuhiko Hayashi
    Wellcome Trust Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Cell Stem Cell 3:391-401. 2008
    ....
  49. ncbi A critical role for Dnmt1 and DNA methylation in T cell development, function, and survival
    P P Lee
    Department of Immunology, University of Washington, Seattle, WA 98195, USA
    Immunity 15:763-74. 2001
    ..and of the maintenance DNA methyltransferase Dnmt1 in the epigenetic regulation of developmental stage- and cell lineage-specific gene expression in vivo is uncertain...
  50. ncbi NOD/SCID/gamma(c)(null) mouse: an excellent recipient mouse model for engraftment of human cells
    Mamoru Ito
    Central Institute for Experimental Animals, Department of Pediatrics, Graduate School of Medicine, Kyoto University, 1430 Nogawa, Miyamae, Kawasaki, Kanagawa 216 0001, Japan
    Blood 100:3175-82. 2002
    ....
  51. ncbi Making or breaking the heart: from lineage determination to morphogenesis
    Deepak Srivastava
    Gladstone Institute of Cardiovascular Disease and Department of Pediatrics and Biochemistry, University of California, San Francisco, CA 94158, USA
    Cell 126:1037-48. 2006
    ....
  52. ncbi FGF stimulation of the Erk1/2 signalling cascade triggers transition of pluripotent embryonic stem cells from self-renewal to lineage commitment
    Tilo Kunath
    Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, UK
    Development 134:2895-902. 2007
    ....
  53. ncbi Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues
    Konrad Hochedlinger
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Cell 121:465-77. 2005
    ..These data show that certain adult progenitors remain competent to interpret key embryonic signals and support the notion that progenitor cells are a driving force in tumorigenesis...
  54. ncbi Stem cells in the hair follicle bulge contribute to wound repair but not to homeostasis of the epidermis
    Mayumi Ito
    Department of Dermatology, Kligman Laboratories, University of Pennsylvania School of Medicine, M8 Stellar Chance Building, 422 Curie Boulevard, Philadelphia, Pennsylvania 19104, USA
    Nat Med 11:1351-4. 2005
    ..Our findings have implications for both gene therapy and developing treatments for wounds because it will be necessary to consider epidermal and hair follicle stem cells as distinct populations...
  55. pmc Blimp1 defines a progenitor population that governs cellular input to the sebaceous gland
    Valerie Horsley
    Laboratory of Mammalian Cell Biology and Development, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Cell 126:597-609. 2006
    ....
  56. ncbi The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells
    Kaoru Mitsui
    Laboratory of Animal Molecular Technology, Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Nara 630 0192, Japan
    Cell 113:631-42. 2003
    ..nanog-deficient ES cells lost pluripotency and differentiated into extraembryonic endoderm lineage. These data demonstrate that Nanog is a critical factor underlying pluripotency in both ICM and ES cells...
  57. pmc Haematopoietic stem cells derive directly from aortic endothelium during development
    Julien Y Bertrand
    Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California 92093 0380, USA
    Nature 464:108-11. 2010
    ..Finally, using a permanent lineage tracing strategy, we demonstrate that the HSCs generated from haemogenic endothelium are the lineal founders of the adult haematopoietic system...
  58. pmc Dendritic cell and macrophage heterogeneity in vivo
    Daigo Hashimoto
    Department of Oncological Sciences, 1425 Madison Avenue, Mount Sinai School of Medicine, New York, NY 10029, USA
    Immunity 35:323-35. 2011
    ..We also emphasize the need to further understand the functional heterogeneity of the tissue DC and macrophage lineages to better comprehend the complex role of these cells in tissue homeostasis and immunity...
  59. pmc A critical role for TCF-1 in T-lineage specification and differentiation
    Brittany Nicole Weber
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Nature 476:63-8. 2011
    ..Our data suggest a model where Notch signals induce TCF-1, and TCF-1 in turn imprints the T-cell fate by upregulating expression of T-cell essential genes...
  60. ncbi Neuronal specification in the spinal cord: inductive signals and transcriptional codes
    T M Jessell
    Howard Hughes Medical Institute, Center for Neurobiology and Behavior, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    Nat Rev Genet 1:20-9. 2000
    ..The mechanisms that specify the identity of neural cells have been examined in many regions of the nervous system and reveal a high degree of conservation in the specification of cell fate by key signalling molecules...
  61. ncbi A Pax3/Pax7-dependent population of skeletal muscle progenitor cells
    Frederic Relaix
    C N R S URA 2578, Department of Developmental Biology, Pasteur Institute, 75724 Paris Cedex 15, France
    Nature 435:948-53. 2005
    ....
  62. ncbi Distinct roles for Hedgehog and canonical Wnt signaling in specification, differentiation and maintenance of osteoblast progenitors
    Stephen J Rodda
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Development 133:3231-44. 2006
    ..These data demonstrate that commitment within the osteoblast lineage requires sequential, stage-specific, Ihh and canonical Wnt/beta-catenin signaling to promote osteogenic, and block chondrogenic, programs of cell fate specification...
  63. ncbi Notch signals control the fate of immature progenitor cells in the intestine
    Silvia Fre
    Department of Cell Biology, Harvard Medical School, Massachusetts General Hospital Cancer Center, Charlestown, Massachusetts 02129, USA
    Nature 435:964-8. 2005
    ..Here, by modulating Notch activity in the mouse intestine, we directly implicate Notch signals in intestinal cell lineage specification...
  64. pmc Stochasticity and cell fate
    Richard Losick
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Science 320:65-8. 2008
    ..Stochasticity has important mechanistic requirements. We speculate on why stochasticity is advantageous-and even critical in some circumstances-to the individual, the colony, or the species...
  65. pmc MicroRNA-mediated conversion of human fibroblasts to neurons
    Andrew S Yoo
    Howard Hughes Medical Institute and Department of Developmental Biology, Stanford University, Stanford, California 94305, USA
    Nature 476:228-31. 2011
    ..These studies indicate that the genetic circuitry involving miR-9/9*-124 can have an instructive role in neural fate determination...
  66. pmc Functional heterogeneity of embryonic stem cells revealed through translational amplification of an early endodermal transcript
    Maurice A Canham
    Institute for Stem Cell Research, Medical Research Council Centre for Regenerative Medicine, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom
    PLoS Biol 8:e1000379. 2010
    ..Taken together our data support a model in which ES cell culture has trapped a set of interconvertible cell states reminiscent of the early stages in blastocyst differentiation that may exist only transiently in the early embryo...
  67. pmc A luminal epithelial stem cell that is a cell of origin for prostate cancer
    Xi Wang
    Department of Medicine, Herbert Irving Comprehensive Cancer Center, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Nature 461:495-500. 2009
    ..These observations indicate that CARNs represent a new luminal stem cell population that is an efficient target for oncogenic transformation in prostate cancer...
  68. pmc Lrig1 expression defines a distinct multipotent stem cell population in mammalian epidermis
    Kim B Jensen
    Laboratory for Epidermal Stem Cell Biology, Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, UK
    Cell Stem Cell 4:427-39. 2009
    ..Our results suggest that Lrig1-positive cells constitute a previously unidentified reservoir of adult mouse interfollicular epidermal stem cells...
  69. ncbi A heterogeneous expression pattern for Nanog in embryonic stem cells
    Amar M Singh
    Department of Pathology, University of Florida College of Medicine, Box 100275, Gainesville, Florida 32610, USA
    Stem Cells 25:2534-42. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  70. ncbi Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants
    David Traver
    Children s Hospital Boston and the Howard Hughes Medical Institute, 320 Longwood Avenue, Enders 720, Boston, Massachusetts 02115, USA
    Nat Immunol 4:1238-46. 2003
    ..Direct visualization of fluorescent donor cells in embryonic recipients allows engraftment and homing events to be imaged in real time. These results provide a cellular context in which to study the genetics of hematopoiesis...
  71. pmc A global network of transcription factors, involving E2A, EBF1 and Foxo1, that orchestrates B cell fate
    Yin C Lin
    Department of Molecular Biology, University of California, San Diego, La Jolla, California, USA
    Nat Immunol 11:635-43. 2010
    ..From this analysis we constructed a global network consisting of transcriptional regulators, signaling and survival factors that we propose orchestrates B cell fate...
  72. doi Blood stem cells emerge from aortic endothelium by a novel type of cell transition
    Karima Kissa
    Institut Pasteur, Unité Macrophages et Développement de l Immunité and CNRS URA2578, 25 rue du Dr Roux, F 75724 Paris Cedex 15, France
    Nature 464:112-5. 2010
    ....
  73. doi Origin and development of dendritic cells
    Kang Liu
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA
    Immunol Rev 234:45-54. 2010
    ..Within tissues and during development, DC division and homeostasis are regulated by the hormone Flt3L...
  74. pmc Cardiomyocytes can be generated from marrow stromal cells in vitro
    S Makino
    Cardiopulmonary Division, Department of Internal Medicine, Keio University School of Medicine, Tokyo 160 8582, Japan
    J Clin Invest 103:697-705. 1999
    ..These cells expressed Nkx2.5/Csx, GATA4, TEF-1, and MEF-2C mRNA before 5-azacytidine treatment and expressed MEF-2A and MEF-2D after treatment. This new cell line provides a powerful model for the study of cardiomyocyte differentiation...
  75. ncbi Differentiation of mouse embryonic stem cells into a defined neuronal lineage
    Miriam Bibel
    Friedrich Miescher Institute, CH 4058 Basel, Switzerland
    Nat Neurosci 7:1003-9. 2004
    ..As they do in vivo, these cells went on to generate neurons with remarkably uniform biochemical and electrophysiological characteristics...
  76. pmc Global transcription in pluripotent embryonic stem cells
    Sol Efroni
    National Cancer Institute Center for Bioinformatics, National Institutes of Health, Rockville, MD 20852, USA
    Cell Stem Cell 2:437-47. 2008
    ..We propose that global transcription is a hallmark of pluripotent ESCs, contributing to their plasticity, and that lineage specification is driven by reduction of the transcribed portion of the genome...
  77. ncbi Foxp3 in control of the regulatory T cell lineage
    Ye Zheng
    Department of Immunology, University of Washington, Seattle, Washington 98195, USA
    Nat Immunol 8:457-62. 2007
    ..Here, we review recent advances in our understanding of the Foxp3-dependent molecular and functional program and the role of Foxp3 in regulatory T cell biology...
  78. pmc Hematopoiesis: an evolving paradigm for stem cell biology
    Stuart H Orkin
    Division of Hematology Oncology, Children s Hospital Boston and the Dana Farber Cancer Institute, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA
    Cell 132:631-44. 2008
    ....
  79. pmc An early T cell lineage commitment checkpoint dependent on the transcription factor Bcl11b
    Long Li
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Science 329:89-93. 2010
    ....
  80. ncbi Multipotent mesenchymal stem cells from adult human synovial membrane
    C De Bari
    Katholieke Universiteit Leuven, Belgium
    Arthritis Rheum 44:1928-42. 2001
    ..SM-derived MSCs may play a role in the regenerative response during arthritic diseases and are promising candidates for developing novel cell-based therapeutic approaches for postnatal skeletal tissue repair...
  81. ncbi Epigenetic reprogramming in mammals
    Hugh D Morgan
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge, UK
    Hum Mol Genet 14:R47-58. 2005
    ..A mechanistic understanding of reprogramming will be crucial for medical applications of stem cell technology...
  82. ncbi Pax7 is required for the specification of myogenic satellite cells
    P Seale
    Department of Biology, McMaster University, Hamilton, Ontario, Canada
    Cell 102:777-86. 2000
    ..Together these studies suggest that induction of Pax7 in muscle-derived stem cells induces satellite cell specification by restricting alternate developmental programs...
  83. doi Induction of functional hepatocyte-like cells from mouse fibroblasts by defined factors
    Pengyu Huang
    Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy for Sciences, Yueyang Road 320, 200031 Shanghai, China
    Nature 475:386-9. 2011
    ..Our study provides a novel strategy to generate functional hepatocyte-like cells for the purpose of liver engineering and regenerative medicine...
  84. ncbi Cortical excitatory neurons and glia, but not GABAergic neurons, are produced in the Emx1-expressing lineage
    Jessica A Gorski
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    J Neurosci 22:6309-14. 2002
    ..Structures that are located near the basal ganglia (e.g., the amygdala and endopiriform nuclei) are not uniformly derived from Emx1-expressing cells...
  85. ncbi Bcl2 regulation by the melanocyte master regulator Mitf modulates lineage survival and melanoma cell viability
    Gael G McGill
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Cell 109:707-18. 2002
    ..This linkage helps explain the vital roles of both Mitf and Bcl2 in the melanocyte lineage and the well-known treatment resistance of melanoma...
  86. pmc Mesenchymal and haematopoietic stem cells form a unique bone marrow niche
    Simon Mendez-Ferrer
    Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
    Nature 466:829-34. 2010
    ..These results uncover an unprecedented partnership between two distinct somatic stem-cell types and are indicative of a unique niche in the bone marrow made of heterotypic stem-cell pairs...
  87. pmc Multipotent cell lineages in early mouse development depend on SOX2 function
    Ariel A Avilion
    Division of Developmental Genetics, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Genes Dev 17:126-40. 2003
    Each cell lineage specified in the preimplantation mammalian embryo depends on intrinsic factors for its development, but there is also mutual interdependence between them...
  88. pmc Acquisition of granule neuron precursor identity is a critical determinant of progenitor cell competence to form Shh-induced medulloblastoma
    Ulrich Schüller
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 14:123-34. 2008
    ..Thus, oncogenic Hh signaling promotes medulloblastoma from lineage-restricted granule cell progenitors...
  89. ncbi Requirement of Math1 for secretory cell lineage commitment in the mouse intestine
    Q Yang
    Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA
    Science 294:2155-8. 2001
    ..The continuous rapid renewal of these cells makes the intestinal epithelium a model system for the study of stem cell regeneration and lineage commitment...
  90. ncbi Bifurcation dynamics in lineage-commitment in bipotent progenitor cells
    Sui Huang
    Department of Surgery and Vascular Biology Program, Children s Hospital, Harvard Medical School, and Harvard Stem Cell Institute, Children s Hospital, 1 Blackfan Circle, Boston, MA 02115, USA
    Dev Biol 305:695-713. 2007
    ....
  91. ncbi Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages
    Laurie E Harrington
    Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Nat Immunol 6:1123-32. 2005
    ..These findings provide a basis for understanding how inhibition of IFN-gamma signaling enhances development of pathogenic T(H)-17 effector cells that can exacerbate autoimmunity...
  92. ncbi Generation of germline-competent induced pluripotent stem cells
    Keisuke Okita
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nature 448:313-7. 2007
    ..Thus, iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application...
  93. ncbi Transforming growth factor-beta induces development of the T(H)17 lineage
    Paul R Mangan
    Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama 35294 2170, USA
    Nature 441:231-4. 2006
    ..The action of TGF-beta on naive T cells is antagonized by interferon-gamma and IL-4, thus providing a mechanism for divergence of the T(H)1, T(H)2 and T(H)17 lineages...
  94. ncbi EGF converts transit-amplifying neurogenic precursors in the adult brain into multipotent stem cells
    Fiona Doetsch
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Neuron 36:1021-34. 2002
    ..Furthermore, purified C cells are 53-fold enriched for neurosphere generation. We conclude that transit-amplifying cells retain stem cell competence under the influence of growth factors...
  95. pmc Epigenetic restriction of embryonic cell lineage fate by methylation of Elf5
    Ray Kit Ng
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge CB22 3AT, UK
    Nat Cell Biol 10:1280-90. 2008
    ..This epigenetic restriction of cell lineage fate provides a molecular mechanism for Waddington's concept of canalization of developmental pathways.
  96. pmc Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow
    Satoru Morikawa
    Department of Physiology, Keio University School of Medicine, Shinjuku ku, Tokyo 160 8582, Japan
    J Exp Med 206:2483-96. 2009
    ..Naive MSCs resided in the perivascular region in a quiescent state. This study provides the useful method needed to identify MSCs as defined in vivo entities...
  97. ncbi Regulatory T cell lineage specification by the forkhead transcription factor foxp3
    Jason D Fontenot
    Department of Immunology, University of Washington, Seattle, Washington 98195, USA
    Immunity 22:329-41. 2005
    ..Here, we present data arguing that the forkhead transcription factor Foxp3 acts as the regulatory T cell lineage specification factor and mediator of the genetic mechanism of dominant tolerance...
  98. pmc Control of regulatory T cell lineage commitment and maintenance
    Steven Z Josefowicz
    Howard Hughes Medical Institute and Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Immunity 30:616-25. 2009
    ..In this review we discuss Treg cell differentiation and maintenance with a particular emphasis on molecular regulation of Foxp3 expression, arguably a key to mechanistic understanding of biology of regulatory T cells...
  99. ncbi Transgenic mice with hematopoietic and lymphoid specific expression of Cre
    Jasper de Boer
    Department of Molecular Immunology, National Institute for Medical Research, The Ridgeway, London, GB
    Eur J Immunol 33:314-25. 2003
    ..Both of these Cre-transgenic lines will be useful in generating tissue-specific gene deletions within all the cells of hematopoietic or lymphoid tissues...
  100. ncbi Monocyte and macrophage heterogeneity
    Siamon Gordon
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Nat Rev Immunol 5:953-64. 2005
    ..These advances in our understanding have implications for the development of therapeutic strategies that are targeted to modify particular subpopulations of monocytes...
  101. doi The transcriptional repressor Bcl-6 directs T follicular helper cell lineage commitment
    Di Yu
    Immunology and Inflammation, Garvan Institute of Medical Research, Sydney, NSW 2010, Australia
    Immunity 31:457-68. 2009
    ..Thus, Bcl-6 positively directs Tfh cell differentiation, through combined repression of miRNAs and transcription factors...

Research Grants73

  1. Role of the Mammalian Siderophore in Cellular Iron Metabolism and Erythropoiesis
    Laxminarayana R Devireddy; Fiscal Year: 2012
    ..We will perform experiments to determine the role of a metazoan siderophore in heme biogenesis and in blood cell lineage specification in developing zebrafish embryos (Aim 3)...
  2. Alternative Macrophage Activation Limits Immunopathology
    DEBROSKI R HERBERT; Fiscal Year: 2011
    ..A greater mechanistic understanding of macrophage effector function on a molecular level of should allow for the design of novel therapeutic approaches to the myriad diseases whose expression is controlled by MF. ..
  3. Autoimmunity Center of Excellence (ACE) at Stanford
    CHARLES GARRISON FATHMAN; Fiscal Year: 2013
    ..Project, centered on CD4 T cell unresponsiveness, and a translational Research Project to study a new T cell lineage (termed Th17 cells) that is characterized by the ability of these lymphocytes to secrete high levels of the ..
  4. Use of umbilical cord as a unique stem cell source for tissue regeneration
    Xiao Dong Chen; Fiscal Year: 2013
    ..originated from 3 embryonic germ layers in vitro under conditions known to induce commitment to a specific cell lineage, including osteoblasts, adipocytes, chondroblasts and cardiomyocytes (mesoderm), nerve (ectoderm) and ..
  5. Regulated Accessibility of a cytokine gene locus.
    Anjana Rao; Fiscal Year: 2013
    ..way, and because DNA methylation is relevant to several fields including mammalian development, cancer, aging, cell lineage specification, genome defense and stem cell function...
  6. DNA Methylation Control Hematopoietic Stem Cell Lineage Differentiation
    GRANT ANTHONY CHALLEN; Fiscal Year: 2013
    ..I believe the basic research outlined in this project can contribute to the fundamental understanding of epigenetic regulation of stem cell function and has direct relevance to progression of human disease states. ..
  7. T-cell Therapy for B-lineage Acute Lymphoblastic Leukemia
    Laurence J N Cooper; Fiscal Year: 2013
    ..chimeric antigen receptor (CAR), designated CD19RCD28, to redirect specificity of T cells to the B-cell lineage-restricted cell-surface molecule CD19 independent of major histocompatibility complex (MHC)...
  8. Stem cell mutagenesis mediated by age and niche
    VIVIENNE ILONA REBEL; Fiscal Year: 2011
    ..in hematopoietic stem cells and compare this to that measured in differentiated cells of the myeloid - and B-cell lineage. By obtaining purified cell populations from mice of 4 age groups, day 14...
  9. Molecular and Cellular Mechanisms of IL-17 Signaling
    Xiaoxia Li; Fiscal Year: 2013
    ..Recent studies have shown that a distinct CD4 T cell lineage -Th17 cells driven by TGF /IL-6/IL-23 play a critical role in the development of EAE...
  10. Mouse models for esophageal Cox-2 oxidative stress and DNA damage
    John P Lynch; Fiscal Year: 2013
    ..This suggests that Cox-2 expression is sufficient to induce an altered cell lineage from keratinocytes, one that has mucin-secretory features consistent with BE cells...
  11. IMMUNOLOGIC BASIS OF RESISTANCE AND HYPERSENSITIVITY
    KARL FRANK AUSTEN; Fiscal Year: 2012
    ..disrupted mice;integrin-ligand and chemokine receptor-ligand interactions in the homing of T cells and of mast cell lineage progenitors;cytokine regulation of eicosanoid biosynthesis in human and mouse culture-derived mast cells;..
  12. Molecular Analysis of GnRH Action
    Mark S Roberson; Fiscal Year: 2012
    ..Studies investigate potential developmental abnormalities associated with the loss of ERKs on cell lineage specification during pituitary organogenesis...
  13. Regenerating Human Oral Mucosa by Bone Marrow-derived Mesenchymal Stem Cells
    RUBIE ANN RAKIAN; Fiscal Year: 2013
    ..the capacity of MSCs maintained on tissue-specific microenvironment to differentiate into an epithelial cell lineage and form oral mucosa epithelium in vitro;2) Demonstrate the capacity of predifferentiated MSCs maintained on ..
  14. THE FUNCTION AND REGULATION OF HISTIDINE DECARBOXYLASE IN GUT INFLAMMATION
    TIMOTHY CRAGIN WANG; Fiscal Year: 2013
    ..HDC-BAC-Cre-ERTM mice developed in our laboratory to trace the development and differentiation of the myeloid cell lineage, both in vitro and in vivo...
  15. Mammary Gland Morphogenesis and Breast Cancer
    Ian G Macara; Fiscal Year: 2013
    ..limit mammary cell proliferation by suppressing Rac activity in the NICD cells, while separately controlling cell lineage determination and metastasis through aPKC...
  16. AID-mediated genetic instability in BCR-ABL1-transformed B cell lineage leukemia
    MARKUS MUSCHEN; Fiscal Year: 2013
    B cell lineage acute lymphoblastic leukemia (ALL) represents the most frequent malignancy in children and is also common in adults. Compared to patients with other malignancies, cure rates for patients with ALL are in general higher...
  17. A Comparative Approach to Globin Regulation/Thalassemia
    LEONARD IRA ZON; Fiscal Year: 2012
    Hemoglobin production is an evolutionarily conserved process that is specific to the red blood cell lineage. We have utilized the zebrafish as a model for studying this process...
  18. Innate and Adaptive Immune Mechanisms in Arthritis
    RICHARD J BUCALA; Fiscal Year: 2013
    ..Such inhibitors may show greatest efficacy in those patients with high-expression MIF alleles and who manifest an MIF-dependent form of inflammatory disease. ..
  19. Germ cell specifications and differentiation in planarians.
    Phillip A Newmark; Fiscal Year: 2013
    ..In planarians, the germ cell lineage develops post- embryonically from the somatic stem cells;like the soma, the germ cell lineage can also be ..
  20. Novel Immunodeficiency caused by TFRC Mutation
    RAIF SALIM GEHA; Fiscal Year: 2013
    ..Steap3 associates with TfR1, thereby promoting its internalization and iron transport, thus sparing the red cell lineage from the effects of the TfR1 mutation in the patients...
  21. Regulation of Apical-Basal Cell polarity during Intestinal Epithelial Morphogenes
    Nan Gao; Fiscal Year: 2013
    ..We hypothesize that apical polarity complex (Par3/Par6/aPKc/Cdc42) incorporates intestinal cell lineage information to instruct polarity formation during epithelial morphogenesis...
  22. Skin-targeted Cell Therapy for Recessive Dystrophic Epidermolysis Bullosa
    Jakub Tolar; Fiscal Year: 2013
    ..basis of biological systems-engineered restriction enzymes (nucleases), skin- targeting, and induced cell lineage conversion (iPSCs)-we will focus on developing personalized cell therapy for individuals with RDEB with the ..
  23. Characterizing a New Human Dendritic Cell Lineage and Its Role in LCH
    Miriam Merad; Fiscal Year: 2013
    ..Future studies will be aimed at utilizing the data acquired here to build mouse models of the disease, and to expand our approach to a larger cohort of patients. ..
  24. Computational Modeling and Accelerated Aging of the Ascending Aorta
    Jay D Humphrey; Fiscal Year: 2013
    ..hypothesize that this unique, cyclic multiaxial loading coupled with the unique underlying micro- structure and cell lineage of the ascending aorta accelerates a mechano-aging process that manifests as the recently reported earliest ..
  25. Cytokine Modulation of Heme Oxygenase-1 in Chronic Kidney Transplant Rejection
    Anupam Agarwal; Fiscal Year: 2010
    ..In Aim 3, the hematopoietic cell lineage required for the effects of IL-10 will be determined using adoptive transfer of specific cell populations from ..
  26. Epigenetic modification of endothelial progenitor cells into multipotent cells
    Rajasingh Johnson; Fiscal Year: 2010
    ..that 1) 5Aza and TSA treatment of EPC induces pluripotent genes Oct4, and Nanog and down regulates endothelial cell lineage specific genes...
  27. Mechanisms of Adult Taste Bud Regeneration
    Linda A Barlow; Fiscal Year: 2013
    ..Aim 1. Define the cell lineage and kinetics of taste receptor cell renewal in adult mice Aim 2...
  28. Pre-B cell receptor signaling in acute lymphoblastic leukemia
    MARKUS MUSCHEN; Fiscal Year: 2013
    ..Likewise, B cell lineage acute lymphoblastic leukemia (ALL) cells are arrested at early stages of B cell development...
  29. The Role of Ets1 in Melanocyte Development
    Lidia Kos; Fiscal Year: 2012
    ..Besides their relevance for understanding fundamental principles of cell lineage specification, our studies will also be relevant for the understanding of developmental disorders involving the ..
  30. Regulation of Spermatocyte Transcription by Testis TAFS
    Margaret T Fuller; Fiscal Year: 2013
    ..Using the dramatic cellular differentiation program of spermatogenesis as a model stem cell lineage, we seek to understand the unique, cell type specific transcription program initiated in the spermatocyte stage ..
  31. The Role of MKP-1 in innate immune responses to LPS
    Yusen Liu; Fiscal Year: 2010
    ..than are wild type mice to endotoxic shock due to exacerbated inflammatory responses by both the hematopoietic cell lineage and cardiomyocytes and resulting damage to the cardiac tissues;2) To test the hypothesis that cholera toxin B ..
  32. MLL in Hematopoiesis and Leukemia in the Zebrafish Model
    Carolyn A Felix; Fiscal Year: 2013
    ..development, and that loss of MLL causes ectopic and asynchronous overexpression and underexpression of blood cell lineage genes and ineffective development of the hematopoietic system...
  33. Regulation of miRNA expression during helper T cell differentiation
    KARL MARK ANSEL; Fiscal Year: 2010
    ..Proper regulation of helper T cell lineage decisions is critical for effective immunity against pathogens and for the prevention of inappropriate ..
  34. Role of ROR??t in the transcriptional regulatory network underlying Th17 lineage
    Dan Littman; Fiscal Year: 2010
    ..Although it has a central role in Th17 cell lineage specification, ROR(t collaborates with several other transcription factors that have also been shown to be ..
  35. EVOLUTIONARY ORIGINS OF IMMUNE-TYPE GENES
    GARY WILLIAM LITMAN; Fiscal Year: 2013
    ..We seek to examine the nature of the variation in these receptors, relate genetic diversity to specific cell lineage(s) and examine the relationships between receptor variation and genome context as a means to gain a more basic ..
  36. TERMINAL DIFFERENTIATION OF THE RENAL EPITHELIUM
    Samir S El-Dahr; Fiscal Year: 2012
    ..We propose that p53 is preferentially required to execute a differentiation program in the ureteric bud cell lineage, and that this function is orchestrated and deciphered by distinct acetylation and phosphorylation cassettes ..
  37. Mechanical Control of Mesenchymal Stem Cell Lineage Allocation
    JANET E RUBIN; Fiscal Year: 2013
    ..We will look at direct effects to prevent adipogenesis (inhibition of PPAR? expression) and indirect [unreadable]- catenin inhibition of PPAR? responses. ..
  38. Maternal Diet and Susceptibility to Neonatal Brain Injury
    John D Barks; Fiscal Year: 2013
    ..now propose to extend our findings and to investigate underlying mechanisms in the systemic and brain myeloid cell lineage. The typical US diet is high in fat (about 34% of calories from fat), with abundant saturated and n6 ..
  39. TOX, a Novel Regulator of Thymocyte Selection
    JONATHAN G KAYE; Fiscal Year: 2013
    ..We produced TOX-deficient mice, demonstrating that TOX is required for development of the canonical CD4 T cell lineage as well as NKT and Treg cell development...
  40. Asb2 in CD4+ T cell lineage differentiation and its plasticity
    Xiao Hong Sun; Fiscal Year: 2013
    ..Fresh ideas will likely emerge from these studies that will be o therapeutic value in the contest of immunity and autoimmunity. ..
  41. Direct Regulation of CD8 T Cells by Interferon gamma
    Marulasiddappa Suresh; Fiscal Year: 2010
    ..by antibody blocking experiments and by developing a transgenic mouse model in which IFNgammaR expression in T-cell lineage can be turned on and off at will, and (3) To determine the effect of IFNgammaR deficiency on the qualitative ..
  42. Development of the renin-expressing cell
    Maria L Sequeira Lopez; Fiscal Year: 2010
    ..will use available mouse models that mark cells derived from the neuroectoderm, the neural crest and the renin cell lineage. To determine whether deletion of the renin gene in neuroectodermal/neural crest/ epidermal precursors results ..
  43. control of Inflammation by regulatory T cells and BCL6
    Alexander L Dent; Fiscal Year: 2012
    ..This work will offer new insights into the molecular regulation of regulatory T cells, thus facilitating the development of new therapies targeting BCL6 for treatment of inflammation and autoimmunity. ) ..
  44. Cytoskeletal Pliability within Cells under Stress: RBCs and Marrow-derived SCs
    Dennis E Discher; Fiscal Year: 2012
    ..more typical cytoskeleton with stress fibers that contribute to actively probing the microenvironment to direct cell lineage. A large number of disease- causing mutants have been identified and some involve protein unfolding...
  45. Targeting_the_Source_of_Persistent_HIV_Viremia
    Sarah Palmer; Fiscal Year: 2010
    ..This will be important for identifying the cell lineage producing virions during suppressive therapy...
  46. Taste organs: formation and regulation
    Hongxiang Liu; Fiscal Year: 2013
    ..Cre- mediated, tissue-specific, genetic labeling and modifications provide powerful tools for these cell lineage assays and functional analyses...
  47. Myeloid Lineage Differentiation and Osteoclast Priming by a Novel Pax5 Cytokine
    Mark C Horowitz; Fiscal Year: 2013
    ..We have begun an analysis of mice deficient in Pax5, a TF required for commitment and maintenance of the B cell lineage. These animals experience a developmental block in B cell differentiation resulting in a phenotype ..
  48. Pancreas Transcription Factors and Disease Model Systems
    Stephen F Konieczny; Fiscal Year: 2013
    ..responsible for establishing cell polarity, protein synthesis functions, and maintenance of the acinar cell lineage. Pancreata from Mist1 null (Mist1KO) mice are characterized by highly disorganized acinar cells that show ..
  49. The role of CBir1 flagellin-specific Th17 and Th1 effector cells in colitis
    Yingzi Cong; Fiscal Year: 2012
    ..by applicant): IL-17-producing CD4+ T (Th17) cells have recently been defined as a separate effector T cell lineage distinct from Th1 and Th2 cells...
  50. The Role of Calcium Signaling in Stem Cell Quiescence
    Gozde Yucel; Fiscal Year: 2010
    ..2 mutant mice. The goal is to examine the cell lineage requiring CaV1.2 function and to examine alterations in known signaling pathways in mutant mice...
  51. The role of ROCKs in stroke recovery
    Qing Mei Wang; Fiscal Year: 2013
    ..ROCK1flox/flox and CD4CreROCK2flox/flox) and investigate the role of ROCK1 and ROCK2 in regulation of T helper cell lineage differentiation in response to oxygen glucose deprivation...
  52. Harnessing mechanical signals to control mesenchymal stem cell fate
    JANET E RUBIN; Fiscal Year: 2013
    ..The ability to define stem cell lineage by mechanical signals suggests that a 'developmental'rather than a 'metabolic'strategy ..
  53. Anti-viral and antileukemic T-cell therapy as prophylaxis after HSCT
    Helen E Heslop; Fiscal Year: 2013
    ..we have designed a chimeric antigen receptor (CAR) to redirect antigen specificity of T cells to the B cell lineage-restricted cell- surface molecule CD19...
  54. GATA Factor Function in Trophoblast
    Soumen Paul; Fiscal Year: 2013
    ..be limiting (in vitro approach), and by knocking-down GATA2 and GATA3 by RNA interference in the trophoblast cell lineage in vivo...
  55. Assay Development for Measuring Chemical Effects on the Epigenome in Stem Cells
    Cheryl A Hobbs; Fiscal Year: 2013
    ..of a panel of human stem cell transcription factors, Polycomb and Trithorax target genes, and markers of cell lineage progression will be screened to identify promoters exhibiting bivalent histone marks in stem cells...
  56. RELM-alpha regulation of hookworm-induced lung inflammation
    MEERA GOH NAIR; Fiscal Year: 2013
    ..However, the factors that promote RELMa expression and the cell lineage-restricted requirements for RELMa-mediated tissue protection are unknown...
  57. Protection of Developing White Matter during Cardiac Surgery
    Richard A Jonas; Fiscal Year: 2013
    ..Immunohistochemistry methods and specifically developed transgenic mice allow identification of the cell lineage leading to mature oligodendrocytes which are responsible for the laying down of myelin...
  58. Mechanistic Studies of Ras-MAPK Signals: Specialzed Cues for the T cell Lineage?
    Jeroen Roose; Fiscal Year: 2011
    ..Our studies will also lead to the generation of novel tools (mouse models and antibodies) that will benefit the scientific (Immunology) research community. ..
  59. Hhex-a Homeobox Gene Necessary for Liver Development
    Clifford W Bogue; Fiscal Year: 2010
    ..Additionally, this research will shed important insight into the pathogenesis of diseases of the biliary tract, including biliary atresia and polycystic liver disease. ..
  60. Molecular Mechanisms of Gonadotrope Differentiation
    Pamela L Mellon; Fiscal Year: 2010
    ..During development, each cell lineage arises in a unique spatial and temporal pattern...
  61. RhoH GTPase in Hematopoiesis and Cancer
    David A Williams; Fiscal Year: 2013
    ..Thus, overall, we hypothesize that functions of RhoH vary with respect to cell lineage and are dependent on distinct motifs within the RhoH protein...
  62. The Role of SRC-1 in Breast Cancer
    Jianming Xu; Fiscal Year: 2013
    ..An EMT- dependent promoter-reporter senor system will be used to trace the EMT cell lineage during the processes of BC cell EMT-MET plasticity and lung Meta...
  63. Tracking Cancer Stem Cell Evolution
    John Welsh; Fiscal Year: 2009
    A current stem cell hypothesis for breast cancer holds that mutations occur in the normal stem cell lineage from which the various differentiated cell types of the breast derive...
  64. Adrenergic Control of Stem Cell Lineage Commitment in Sepsis
    KUZHALI MUTHUMALAIAPPAN; Fiscal Year: 2009
    ..Since all immune cells are formed in the bone marrow from hematopoietic stem cells, we are studying the effect of catecholamine in the development and function of hematopoietic stem cells in critical burn injury. ..
  65. APOPTOSIS AND REPRODUCTIVE AGING OF THE FEMALE
    Jonathan Tilly; Fiscal Year: 2003
    ..unit of the ovary is the follicle, a structure composed of a single germ cell (oocyte) surrounded by a somatic cell lineage, referred to as granulosa cells (GC), that is the source of estrogens produced by the ovaries...
  66. ANALYSIS OF CELL LINEAGE IN EMBRYOS
    Roger Pedersen; Fiscal Year: 1992
    ..b>Cell lineage analysis will be performed using microinjected tracers at cleavage and gastrula stages...
  67. Positive and Negative Regulatory Pathways In Human Lymphoma
    Richard Ford; Fiscal Year: 2007
    ..by applicant): Non-Hodgkin's Lymphomas (NHL) are common tumors of the human immune system, primarily of B cell lineage (NHL-B) that have been showing significant unexplained increases in incidence for the last three decades...
  68. MOLECULAR GENETICS OF INTESTINAL DEVELOPMENT
    Jeffrey Gordon; Fiscal Year: 1999
    ..The mechanisms that control cell lineage-specific, differentiation-dependent, region-appropriate and temporal patterns of gene expression in the ..
  69. Antibodies Targeting Novel Surface Antigens on Pluripotent Stem Cell Derivatives
    David Larocca; Fiscal Year: 2009
    ..both spatially, in the cell types formed and temporally, in their degree of progression along their fated cell lineage. Even when cell culture conditions are manipulated to direct differentiation toward specific lineages there are ..
  70. NK AND T-CELL DEVELOPMENT FROM HEMATOPOIETIC STEM CELLS
    Jeffrey Miller; Fiscal Year: 1999
    ..and extracellular matrix (ECM) components are responsible for inducing differentiation along the NK or T-cell lineage. Acquisition of CD7 on adult human CD34+ marrow cells correlates with CD3z transcription, a pattern observed in ..
  71. CELL LINEAGE COMMITMENT IN MAMMALIAN MYOGENESIS
    Jeffrey Miller; Fiscal Year: 1991
    ..Methods will include clonal cell culture, neural tissue/somite co-culture, purification of cell types from neural tissue, immunocytochemistry, Western blotting, quantitative Northern blotting, and RNase protection assays...
  72. VITAMIN A SIGNALING AND CARDIAC MORPHOGENIC DEFECTS
    Kenneth Chien; Fiscal Year: 2003
    ..of cardiovascular morphogenesis by creating mice which harbor an early, complete atrial and ventricular muscle cell lineage restricted deficiency in RXRalpha via CRE-lox technology; 2) to determine the role of RXRalpha pathways within ..
  73. Nutritional mechanisms and intestinal tumorigenesis
    Leonard Augenlicht; Fiscal Year: 2009
    ..in the mechanisms of lineage specific differentiation, and more specifically, the differentiation of the goblet cell lineage, determine the probability of tumor initiation, and can be modulated by diet...