cell differentiation

Summary

Summary: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.

Top Publications

  1. ncbi Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 126:663-76. 2006
  2. ncbi Induction of pluripotent stem cells from adult human fibroblasts by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 131:861-72. 2007
  3. pmc Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation
    Cole Trapnell
    Department of Computer Science, University of Maryland, College Park, Maryland, USA
    Nat Biotechnol 28:511-5. 2010
  4. ncbi Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement
    M Dominici
    Laboratory of Cell Biology and Advanced Cancer Therapy, Oncology Hematology Department, University of Modena and Reggio Emilia, Modena, Italy
    Cytotherapy 8:315-7. 2006
  5. pmc The basics of epithelial-mesenchymal transition
    Raghu Kalluri
    Division of Matrix Biology, Beth Israel Deaconess Medical Center, and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Clin Invest 119:1420-8. 2009
  6. ncbi Multilineage potential of adult human mesenchymal stem cells
    M F Pittenger
    Osiris Therapeutics, 2001 Aliceanna Street, Baltimore, MD 21231 3043, USA
    Science 284:143-7. 1999
  7. ncbi Monocyte and macrophage heterogeneity
    Siamon Gordon
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Nat Rev Immunol 5:953-64. 2005
  8. ncbi Identification and expansion of human colon-cancer-initiating cells
    Lucia Ricci-Vitiani
    Department of Hematology and Oncology, Istituto Superiore di Sanita, Viale Regina Elena 299, Rome 00161, Italy
    Nature 445:111-5. 2007
  9. ncbi The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells
    Ivaylo I Ivanov
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
    Cell 126:1121-33. 2006
  10. ncbi Epithelial-mesenchymal transitions in tumour progression
    Jean Paul Thiery
    Centre National Recherche Scientifique Unité Mixte Recherche, 144 Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Nat Rev Cancer 2:442-54. 2002

Detail Information

Publications343 found, 100 shown here

  1. ncbi Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 126:663-76. 2006
    ..These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors...
  2. ncbi Induction of pluripotent stem cells from adult human fibroblasts by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 131:861-72. 2007
    ..Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts...
  3. pmc Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation
    Cole Trapnell
    Department of Computer Science, University of Maryland, College Park, Maryland, USA
    Nat Biotechnol 28:511-5. 2010
    ..These results suggest that Cufflinks can illuminate the substantial regulatory flexibility and complexity in even this well-studied model of muscle development and that it can improve transcriptome-based genome annotation...
  4. ncbi Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement
    M Dominici
    Laboratory of Cell Biology and Advanced Cancer Therapy, Oncology Hematology Department, University of Modena and Reggio Emilia, Modena, Italy
    Cytotherapy 8:315-7. 2006
    ....
  5. pmc The basics of epithelial-mesenchymal transition
    Raghu Kalluri
    Division of Matrix Biology, Beth Israel Deaconess Medical Center, and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Clin Invest 119:1420-8. 2009
    ..The identification of the signaling pathways that lead to activation of EMT programs during these disease processes is providing new insights into the plasticity of cellular phenotypes and possible therapeutic interventions...
  6. ncbi Multilineage potential of adult human mesenchymal stem cells
    M F Pittenger
    Osiris Therapeutics, 2001 Aliceanna Street, Baltimore, MD 21231 3043, USA
    Science 284:143-7. 1999
    ..These adult stem cells could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages. Individual stem cells were identified that, when expanded to colonies, retained their multilineage potential...
  7. ncbi Monocyte and macrophage heterogeneity
    Siamon Gordon
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Nat Rev Immunol 5:953-64. 2005
    ..These advances in our understanding have implications for the development of therapeutic strategies that are targeted to modify particular subpopulations of monocytes...
  8. ncbi Identification and expansion of human colon-cancer-initiating cells
    Lucia Ricci-Vitiani
    Department of Hematology and Oncology, Istituto Superiore di Sanita, Viale Regina Elena 299, Rome 00161, Italy
    Nature 445:111-5. 2007
    ..We conclude that colorectal cancer is created and propagated by a small number of undifferentiated tumorigenic CD133+ cells, which should therefore be the target of future therapies...
  9. ncbi The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells
    Ivaylo I Ivanov
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
    Cell 126:1121-33. 2006
    ..Together, these studies suggest that RORgammat is a key regulator of immune homeostasis and highlight its potential as a therapeutic target in inflammatory diseases...
  10. ncbi Epithelial-mesenchymal transitions in tumour progression
    Jean Paul Thiery
    Centre National Recherche Scientifique Unité Mixte Recherche, 144 Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Nat Rev Cancer 2:442-54. 2002
  11. pmc Epigenetic memory in induced pluripotent stem cells
    K Kim
    Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 467:285-90. 2010
    ....
  12. doi IL-17 and Th17 Cells
    Thomas Korn
    Technical University Munich, Department of Neurology, 81675 Munich, Germany
    Annu Rev Immunol 27:485-517. 2009
    ..effector function, and regulation of Th17 cells has opened up a new framework for understanding T cell differentiation. Furthermore, we now appreciate the importance of Th17 cells in clearing pathogens during host defense ..
  13. ncbi Generation of germline-competent induced pluripotent stem cells
    Keisuke Okita
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nature 448:313-7. 2007
    ..Thus, iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application...
  14. pmc Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells
    Ryan Lister
    Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Nature 471:68-73. 2011
    ..Lastly, differentiation of iPSCs into trophoblast cells revealed that errors in reprogramming CG methylation are transmitted at a high frequency, providing an iPSC reprogramming signature that is maintained after differentiation...
  15. ncbi Matrix elasticity directs stem cell lineage specification
    Adam J Engler
    Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, 19104, USA
    Cell 126:677-89. 2006
    ..The results have significant implications for understanding physical effects of the in vivo microenvironment and also for therapeutic uses of stem cells...
  16. ncbi The chemokine system in diverse forms of macrophage activation and polarization
    Alberto Mantovani
    Centro di Eccellenza per l Innovazione Diagnostica e Terapeutica IDET, Institute of General Pathology, University of Milan, I 20133 Milan, Italy Istituto di Ricerche Farmacologiche Mario Negri, I 20157 Milan, Italy
    Trends Immunol 25:677-86. 2004
    ....
  17. ncbi Mammalian neural stem cells
    F H Gage
    The Salk Institute, Laboratory of Genetics, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 287:1433-8. 2000
    ..Before the full potential of neural stem cells can be realized, we need to learn what controls their proliferation, as well as the various pathways of differentiation available to their daughter cells...
  18. doi Mechanisms and functional implications of adult neurogenesis
    Chunmei Zhao
    Laboratory of Genetics, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Cell 132:645-60. 2008
    ..We further address the potential significance of adult neurogenesis in memory, depression, and neurodegenerative disorders such as Alzheimer's and Parkinson's disease...
  19. pmc Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA
    Luigi Warren
    Immune Disease Institute, Program in Cellular and Molecular Medicine, Children s Hospital Boston, Boston, MA 02115, USA
    Cell Stem Cell 7:618-30. 2010
    ..This technology represents a safe, efficient strategy for somatic cell reprogramming and directing cell fate that has broad applicability for basic research, disease modeling, and regenerative medicine...
  20. pmc A distinct lineage of CD4 T cells regulates tissue inflammation by producing interleukin 17
    Heon Park
    Department of Immunology, University of Washington, Seattle, Washington 98195, USA
    Nat Immunol 6:1133-41. 2005
    ..Thus, IL-17 expression characterizes a unique T helper lineage that regulates tissue inflammation...
  21. ncbi In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
    Marius Wernig
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nature 448:318-24. 2007
    ..Our results show that the biological potency and epigenetic state of in-vitro-reprogrammed induced pluripotent stem cells are indistinguishable from those of ES cells...
  22. ncbi Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells
    Ian Chambers
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, Scotland
    Cell 113:643-55. 2003
    ..These findings establish a central role for Nanog in the transcription factor hierarchy that defines ES cell identity...
  23. ncbi Marrow stromal cells as stem cells for nonhematopoietic tissues
    D J Prockop
    Center for Gene Therapy, Allegheny University of the Health Sciences, MCP Hahnemann School of Medicine, 245 North 15 Street, Mail Stop 421, Philadelphia, PA 19102, USA
    Science 276:71-4. 1997
    ..Therefore, marrow stromal cells present an intriguing model for examining the differentiation of stem cells. Also, they have several characteristics that make them potentially useful for cell and gene therapy...
  24. ncbi The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression
    A Cano
    Instituto de Investigaciones Biomedicas, Arturo Duperier 4, 28029 Madrid, Spain
    Nat Cell Biol 2:76-83. 2000
    ..Snail may thus be considered as a marker for malignancy, opening up new avenues for the design of specific anti-invasive drugs...
  25. ncbi Reprogramming of human somatic cells to pluripotency with defined factors
    In Hyun Park
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 451:141-6. 2008
    ..These data demonstrate that defined factors can reprogramme human cells to pluripotency, and establish a method whereby patient-specific cells might be established in culture...
  26. ncbi Multilineage cells from human adipose tissue: implications for cell-based therapies
    P A Zuk
    Laboratory for Regenerative Bioengineering and Repair, UCLA School of Medicine, Los Angeles, California, USA
    Tissue Eng 7:211-28. 2001
    ..In conclusion, the data support the hypothesis that a human lipoaspirate contains multipotent cells and may represent an alternative stem cell source to bone marrow-derived MSCs...
  27. ncbi Prospective identification of tumorigenic prostate cancer stem cells
    Anne T Collins
    Yorkshire Cancer Research Unit, Department of Biology, University of York, York, United Kingdom
    Cancer Res 65:10946-51. 2005
    ..The identification of a prostate cancer stem cell provides a powerful tool to investigate the tumorigenic process and to develop therapies targeted to the stem cell...
  28. ncbi Pluripotency of mesenchymal stem cells derived from adult marrow
    Yuehua Jiang
    Stem Cell Institute, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Nature 418:41-9. 2002
    ..As MAPCs proliferate extensively without obvious senescence or loss of differentiation potential, they may be an ideal cell source for therapy of inherited or degenerative diseases...
  29. ncbi Osteoclast differentiation and activation
    William J Boyle
    Protein Pathways, Inc, Woodland Hills, California 91367, USA
    Nature 423:337-42. 2003
    ..Further study of this pathway is providing the molecular basis for developing therapeutics to treat osteoporosis and other diseases of bone loss...
  30. ncbi Blood monocytes consist of two principal subsets with distinct migratory properties
    Frederic Geissmann
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
    Immunity 19:71-82. 2003
    ..These findings raise the potential for novel therapeutic strategies in inflammatory diseases...
  31. pmc Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts
    Akiko Doi
    Center for Epigenetics and Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Nat Genet 41:1350-3. 2009
    ....
  32. pmc Histone H3K27ac separates active from poised enhancers and predicts developmental state
    Menno P Creyghton
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 107:21931-6. 2010
    ..Furthermore, poised enhancer networks provide clues to unrealized developmental programs. Finally, we show that enhancers are reset during nuclear reprogramming...
  33. pmc Mesenchymal and haematopoietic stem cells form a unique bone marrow niche
    Simon Mendez-Ferrer
    Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
    Nature 466:829-34. 2010
    ..These results uncover an unprecedented partnership between two distinct somatic stem-cell types and are indicative of a unique niche in the bone marrow made of heterotypic stem-cell pairs...
  34. ncbi Induced pluripotent stem cell lines derived from human somatic cells
    Junying Yu
    Genome Center of Wisconsin, Madison, WI 53706 1580, USA
    Science 318:1917-20. 2007
    ....
  35. ncbi Complex networks orchestrate epithelial-mesenchymal transitions
    Jean Paul Thiery
    Centre National de la Recherche Scientifique CNRS Unité Mixte Recherche UMR 144 and Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Nat Rev Mol Cell Biol 7:131-42. 2006
    ..Understanding how mesenchymal cells arise from an epithelial default status will also have a strong impact in unravelling the mechanisms that control fibrosis and cancer progression...
  36. doi The ground state of embryonic stem cell self-renewal
    Qi Long Ying
    Center for Stem Cell and Regenerative Medicine, Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, 1501 San Pablo Street, ZNI 529, Los Angeles, California 90033, USA
    Nature 453:519-23. 2008
    ..This property may account for their latent tumorigenicity. The delineation of minimal requirements for self-renewal now provides a defined platform for the precise description and dissection of the pluripotent state...
  37. ncbi Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation
    D L Lacey
    Department of Pathology, Amgen, Inc, Thousand Oaks, California 91320 1789, USA
    Cell 93:165-76. 1998
    ..These data suggest that OPGL is an osteoclast differentiation and activation factor. The effects of OPGL are blocked in vitro and in vivo by OPG, suggesting that OPGL and OPG are key extracellular regulators of osteoclast development...
  38. ncbi Identification and expansion of the tumorigenic lung cancer stem cell population
    A Eramo
    Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanita, Rome, Italy
    Cell Death Differ 15:504-14. 2008
    ..Molecular and functional characterization of such a tumorigenic population may provide valuable information to be exploited in the clinical setting...
  39. ncbi Alternative activation of macrophages
    Siamon Gordon
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Nat Rev Immunol 3:23-35. 2003
    ..In this review, I assess the evidence in favour of alternative macrophage activation in the light of macrophage heterogeneity, and define its limits and relevance to a range of immune and inflammatory conditions...
  40. ncbi Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue
    Susanne Kern
    Institute of Transfusion Medicine and Immunology, German Red Cross Blood Service of Baden Württemberg Hessen, Mannheim, Germany
    Stem Cells 24:1294-301. 2006
    ..Both UCB and AT are attractive alternatives to BM in isolating MSC: AT as it contains MSCs at the highest frequency and UCB as it seems to be expandable to higher numbers...
  41. ncbi Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts
    T Komori
    Department of Medicine III, Osaka University Medical School, Suita, Japan
    Cell 89:755-64. 1997
    ....
  42. ncbi Epigenetic reprogramming in mammalian development
    W Reik
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge CB2 4AT, UK
    Science 293:1089-93. 2001
    ..A role of reprogramming in stem cell differentiation is also envisaged...
  43. ncbi A novel transcription factor, T-bet, directs Th1 lineage commitment
    S J Szabo
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Cell 100:655-69. 2000
    ..Thus, T-bet initiates Th1 lineage development from naive Thp cells both by activating Th1 genetic programs and by repressing the opposing Th2 programs...
  44. ncbi The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells
    Kaoru Mitsui
    Laboratory of Animal Molecular Technology, Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Nara 630 0192, Japan
    Cell 113:631-42. 2003
    ..nanog-deficient ES cells lost pluripotency and differentiated into extraembryonic endoderm lineage. These data demonstrate that Nanog is a critical factor underlying pluripotency in both ICM and ES cells...
  45. ncbi RAS is regulated by the let-7 microRNA family
    Steven M Johnson
    Department of Molecular, Cellular and Developmental Biology, Yale University, P O Box 208103, New Haven, CT 06520, USA
    Cell 120:635-47. 2005
    ..let-7 expression is lower in lung tumors than in normal lung tissue, while RAS protein is significantly higher in lung tumors, providing a possible mechanism for let-7 in cancer...
  46. pmc Differentiation of effector CD4 T cell populations (*)
    Jinfang Zhu
    Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 1892, USA
    Annu Rev Immunol 28:445-89. 2010
    ..for their development; the networks of transcription factors involved in their differentiation; the epigenetic regulation of their key cytokines and transcription factors; and human diseases involving defective CD4 T cell differentiation.
  47. pmc Human induced pluripotent stem cells free of vector and transgene sequences
    Junying Yu
    Morgridge Institute for Research, Madison, WI 53707 7365, USA
    Science 324:797-801. 2009
    ..These results demonstrate that reprogramming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors and removes one obstacle to the clinical application of human iPS cells...
  48. ncbi Concise review: mesenchymal stem cells: their phenotype, differentiation capacity, immunological features, and potential for homing
    Giselle Chamberlain
    Leopold Muller Arthritis Research Centre, School of Medicine, Keele University, Robert Jones and Agnes Hunt Orthopaedic Hospital, Oswestry, Shrops SY10 7AG, UK
    Stem Cells 25:2739-49. 2007
    ..The current review describes what is known about MSCs and their capacity to home to tissues together with the associated molecular mechanisms involving chemokine receptors and adhesion molecules...
  49. doi Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation
    Gabriella Ficz
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge CB22 3AT, UK
    Nature 473:398-402. 2011
    ..We propose that the balance between hydroxymethylation and methylation in the genome is inextricably linked with the balance between pluripotency and lineage commitment...
  50. doi MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation
    Yvonne Tay
    Stem Cell and Developmental Biology, Genome Institute of Singapore, Agency for Science Technology and Research, 08 01, Genome, 60 Biopolis Street, Singapore 138672, Singapore
    Nature 455:1124-8. 2008
    ....
  51. ncbi Bone morphogenetic proteins
    Di Chen
    School of Medicine and Dentistry, Department of Orthopaedics, University of Rochester, Rochester, NY 14642, USA
    Growth Factors 22:233-41. 2004
    ..A significant advancement about the understanding of in vivo functions of BMP ligands, receptors and signaling molecules has been achieved in recent years...
  52. ncbi let-7 regulates self renewal and tumorigenicity of breast cancer cells
    Fengyan Yu
    Department of Breast Surgery, No 2 Affiliated Hospital, Sun Yat Sen University, Guangzhou 510120, People s Republic of China
    Cell 131:1109-23. 2007
    ..Therefore let-7 regulates multiple BT-IC stem cell-like properties by silencing more than one target...
  53. ncbi SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells
    Mark J Kiel
    Howard Hughes Medical Institute and Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109, USA
    Cell 121:1109-21. 2005
    ..Many HSCs were associated with sinusoidal endothelium in spleen and bone marrow, though some HSCs were associated with endosteum. HSCs thus occupy multiple niches, including sinusoidal endothelium in diverse tissues...
  54. pmc The epithelial-mesenchymal transition: new insights in signaling, development, and disease
    Jonathan M Lee
    Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada
    J Cell Biol 172:973-81. 2006
    ..E.W. Thompson, and D.F. Newgreen. 2005. Cancer Res. 65:5996-6000; Thompson, E.W., D.F. Newgreen, and D. Tarin. 2005. Cancer Res. 65:5991-5995)...
  55. pmc Parkinson's disease patient-derived induced pluripotent stem cells free of viral reprogramming factors
    Frank Soldner
    The Whitehead Institute, Cambridge Center, MA 02142, USA
    Cell 136:964-77. 2009
    ..Our results indicate that residual transgene expression in virus-carrying hiPSCs can affect their molecular characteristics and that factor-free hiPSCs therefore represent a more suitable source of cells for modeling of human disease...
  56. ncbi Human mesenchymal stem cells modulate B-cell functions
    Anna Corcione
    Laboratory of Oncology, G Gaslini Institute, Largo G Gaslini 5, 16148 Genova, Italy
    Blood 107:367-72. 2006
    ..hMSCs inhibited B-cell differentiation because IgM, IgG, and IgA production was significantly impaired...
  57. pmc Expression of Helios, an Ikaros transcription factor family member, differentiates thymic-derived from peripherally induced Foxp3+ T regulatory cells
    Angela M Thornton
    Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 184:3433-41. 2010
    ..Collectively, these results demonstrate that Helios is potentially a specific marker of thymic-derived Treg cells and raises the possibility that a significant percentage of Foxp3(+) Treg cells are generated extrathymically...
  58. pmc Convergence of Wnt, beta-catenin, and cadherin pathways
    W James Nelson
    Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
    Science 303:1483-7. 2004
    ..Here, we assemble evidence of possible interrelations between Wnt and other growth factor signaling, beta-catenin functions, and cadherin-mediated adhesion...
  59. ncbi Central memory and effector memory T cell subsets: function, generation, and maintenance
    Federica Sallusto
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    Annu Rev Immunol 22:745-63. 2004
    ..This review addresses the heterogeneity of TCM and TEM, their differentiation stages, and the current models for their generation and maintenance in humans and mice...
  60. ncbi Mesenchymal stem cells as trophic mediators
    Arnold I Caplan
    Department of Biology, Skeletal Research Center, Case Western Reserve University, 2080 Adelbert Road, Cleveland, OH 44106 7080, USA
    J Cell Biochem 98:1076-84. 2006
    ..Several studies which tested the use of MSCs in models of infarct (injured heart), stroke (brain), or meniscus regeneration models are reviewed within the context of MSC-mediated trophic effects in tissue repair...
  61. pmc Induced pluripotent stem cells from a spinal muscular atrophy patient
    Allison D Ebert
    The Waisman Center, The Stem Cell and Regenerative Medicine Center, University of Wisconsin Madison, 1500 Highland Avenue, Madison, Wisconsin 53705, USA
    Nature 457:277-80. 2009
    ..As such, it represents a promising resource to study disease mechanisms, screen new drug compounds and develop new therapies...
  62. doi Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons
    John T Dimos
    Harvard Stem Cell Institute, Stowers Medical Institute, Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
    Science 321:1218-21. 2008
    ..These patient-specific iPS cells possess properties of embryonic stem cells and were successfully directed to differentiate into motor neurons, the cell type destroyed in ALS...
  63. ncbi Adult cardiac stem cells are multipotent and support myocardial regeneration
    Antonio P Beltrami
    Cardiovascular Research Institute, Department of Medicine, New York Medical College, Valhalla, NY 10595, USA
    Cell 114:763-76. 2003
    ..The existence of these cells opens new opportunities for myocardial repair...
  64. ncbi New cell lines from mouse epiblast share defining features with human embryonic stem cells
    Paul J Tesar
    Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 448:196-9. 2007
    ..These results show that epiblast cells can be maintained as stable cell lines and interrogated to understand how pluripotent cells generate distinct fates during early development...
  65. pmc A unique chromatin signature uncovers early developmental enhancers in humans
    Alvaro Rada-Iglesias
    Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, California 94305, USA
    Nature 470:279-83. 2011
    ..Moreover, the wealth of new regulatory sequences identified here provides an invaluable resource for studies and isolation of transient, rare cell populations representing early stages of human embryogenesis...
  66. pmc Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation
    Jason Wray
    Wellcome Trust Centre for Stem Cell Research and Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Nat Cell Biol 13:838-45. 2011
    ..We conclude that Gsk3 inhibition stabilizes the embryonic stem cell state primarily by reducing repressive influence on the core pluripotency network...
  67. pmc Generation of pathogenic T(H)17 cells in the absence of TGF-β signalling
    Kamran Ghoreschi
    Molecular Immunology and Inflammation Branch, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 467:967-71. 2010
    ..These data indicate an alternative mode for T(H)17 differentiation. Consistent with genetic data linking IL23R with autoimmunity, our findings re-emphasize the importance of IL-23 and therefore may have therapeutic implications...
  68. pmc The glial nature of embryonic and adult neural stem cells
    Arnold Kriegstein
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Department of Neurology, University of California, San Francisco, California 94143 0525, USA
    Annu Rev Neurosci 32:149-84. 2009
    ..Identification of NSCs and IPCs is critical to understand brain development and adult neurogenesis and to develop new strategies for brain repair...
  69. ncbi Bone marrow cells regenerate infarcted myocardium
    D Orlic
    Hematopoiesis Section, Genetics and Molecular Biology Branch, NHGRI, NIH, Bethesda, MD 20892, USA
    Nature 410:701-5. 2001
    ..organ is sensed by distant stem cells, which migrate to the site of damage and undergo alternate stem cell differentiation; these events promote structural and functional repair...
  70. ncbi Development, cytokine profile and function of human interleukin 17-producing helper T cells
    Nicholas J Wilson
    Department of Discovery Research, Schering Plough Biopharma Formerly DNAX Research, Palo Alto, California 94304 1104, USA
    Nat Immunol 8:950-7. 2007
    ..Our data collectively indicate that human and mouse T(H)-17 cells require distinct factors during differentiation and that human T(H)-17 cells may regulate innate immunity in epithelial cells...
  71. ncbi Embryonic stem cell lines derived from human blastocysts
    J A Thomson
    Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, WI 53715, USA
    Science 282:1145-7. 1998
    ..These cell lines should be useful in human developmental biology, drug discovery, and transplantation medicine...
  72. ncbi Macrophage polarization: tumor-associated macrophages as a paradigm for polarized M2 mononuclear phagocytes
    Alberto Mantovani
    Istituto di Ricerche Farmacologiche Mario Negri, Via Eritrea 62, I 20157 Milan, Italy
    Trends Immunol 23:549-55. 2002
    ..These functionally polarized cells, and similarly oriented or immature dendritic cells present in tumors, have a key role in subversion of adaptive immunity and in inflammatory circuits that promote tumor growth and progression...
  73. doi Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution
    Nimet Maherali
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Harvard Stem Cell Institute, 185 Cambridge Street, Boston, MA 02114, USA
    Cell Stem Cell 1:55-70. 2007
    ....
  74. pmc Cell type of origin influences the molecular and functional properties of mouse induced pluripotent stem cells
    Jose M Polo
    Howard Hughes Medical Institute and Department of Stem Cell and Regenerative Biology, Harvard University and Harvard Medical School, Cambridge, Massachusetts, USA
    Nat Biotechnol 28:848-55. 2010
    ..These observations may influence ongoing attempts to use iPSCs for disease modeling and could also be exploited in potential therapeutic applications to enhance differentiation into desired cell lineages...
  75. pmc piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells
    Knut Woltjen
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    Nature 458:766-70. 2009
    ..We anticipate that the unique properties of this virus-independent simplification of iPS cell production will accelerate this field further towards full exploration of the reprogramming process and future cell-based therapies...
  76. ncbi OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis
    Y Y Kong
    Amgen Institute, Department of Medical Biophysics, University of Toronto, Ontario, Canada
    Nature 397:315-23. 1999
    ..Thus OPGL is a new regulator of lymph-node organogenesis and lymphocyte development and is an essential osteoclast differentiation factor in vivo...
  77. pmc TGF-beta-induced Foxp3 inhibits T(H)17 cell differentiation by antagonizing RORgammat function
    Liang Zhou
    The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, New York 10016, USA
    Nature 453:236-40. 2008
    ..Here we demonstrate that, together with pro-inflammatory cytokines, TGF-beta orchestrates T(H)17 cell differentiation in a concentration-dependent manner...
  78. pmc Induction of intestinal Th17 cells by segmented filamentous bacteria
    Ivaylo I Ivanov
    Molecular Pathogenesis Program, The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA
    Cell 139:485-98. 2009
    ..Thus, manipulation of this commensal-regulated pathway may provide new opportunities for enhancing mucosal immunity and treating autoimmune disease...
  79. pmc Human adipose tissue is a source of multipotent stem cells
    Patricia A Zuk
    Department of Surgery and Orthopedics, Regenerative Bioengineering and Repair Laboratory, UCLA School of Medicine, Los Angeles, California 90095, USA
    Mol Biol Cell 13:4279-95. 2002
    ..Finally, PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression...
  80. pmc Pluripotency and cellular reprogramming: facts, hypotheses, unresolved issues
    Jacob H Hanna
    The Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Cell 143:508-25. 2010
    ..Here we review recent advances in this rapidly moving field and emphasize unresolved and controversial questions...
  81. ncbi Transforming growth factor-beta induces development of the T(H)17 lineage
    Paul R Mangan
    Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama 35294 2170, USA
    Nature 441:231-4. 2006
    ..The action of TGF-beta on naive T cells is antagonized by interferon-gamma and IL-4, thus providing a mechanism for divergence of the T(H)1, T(H)2 and T(H)17 lineages...
  82. doi Long non-coding RNAs: new players in cell differentiation and development
    Alessandro Fatica
    Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
    Nat Rev Genet 15:7-21. 2014
    ..In this Review, we describe the function of lncRNAs in developmental processes, such as in dosage compensation, genomic imprinting, cell differentiation and organogenesis, with a particular emphasis on mammalian development.
  83. ncbi Reciprocal TH17 and regulatory T cell differentiation mediated by retinoic acid
    Daniel Mucida
    La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, CA 92037, USA
    Science 317:256-60. 2007
    ..inhibiting the IL-6-driven induction of proinflammatory T(H)17 cells and promoting anti-inflammatory Treg cell differentiation. These findings indicate that a common metabolite can regulate the balance between pro- and anti-..
  84. pmc Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling
    Stuart M Chambers
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, New York, New York 10065, USA
    Nat Biotechnol 27:275-80. 2009
    ..Noggin/SB431542-based neural induction should facilitate the use of hES and hiPS cells in regenerative medicine and disease modeling and obviate the need for protocols based on stromal feeders or embryoid bodies...
  85. ncbi A bivalent chromatin structure marks key developmental genes in embryonic stem cells
    Bradley E Bernstein
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Cell 125:315-26. 2006
    ..These results highlight the importance of DNA sequence in defining the initial epigenetic landscape and suggest a novel chromatin-based mechanism for maintaining pluripotency...
  86. pmc Biomarkers for epithelial-mesenchymal transitions
    Michael Zeisberg
    Division of Matrix Biology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Clin Invest 119:1429-37. 2009
    ....
  87. ncbi The versatility and universality of calcium signalling
    M J Berridge
    The Babraham Institute, Laboratory of Molecular Signalling, Babraham Hall, Babraham, Cambridge, CB2 4AT, UK
    Nat Rev Mol Cell Biol 1:11-21. 2000
    ..Exceeding its normal spatial and temporal boundaries can result in cell death through both necrosis and apoptosis...
  88. ncbi Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
    Masato Nakagawa
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nat Biotechnol 26:101-6. 2008
    ..The protocol also enabled efficient isolation of iPS cells without drug selection. Furthermore, we generated human iPS cells from adult dermal fibroblasts without MYC...
  89. pmc mTOR regulates memory CD8 T-cell differentiation
    Koichi Araki
    Emory Vaccine Center and Department of Microbiology and Immunology, Atlanta, Georgia, USA
    Nature 460:108-12. 2009
    ..we show that mTOR (mammalian target of rapamycin, also known as FRAP1) is a major regulator of memory CD8 T-cell differentiation, and in contrast to what we expected, the immunosuppressive drug rapamycin has immunostimulatory effects on ..
  90. ncbi Molecular regulation of vascular smooth muscle cell differentiation in development and disease
    Gary K Owens
    Dept of Molecular Physiology and Biological Physics, Univ of Virginia School of Medicine, 415 Lane Rd, Medical Research Building 5, Rm 1220, PO Box 801394, Charlottesville, VA 22908, USA
    Physiol Rev 84:767-801. 2004
    ..The goal of this review is to summarize the current state of our knowledge in this area and to attempt to identify some of the key unresolved challenges and questions that require further study...
  91. ncbi Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment
    Rowena McBeath
    The Cellular and Molecular Medicine Program, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Dev Cell 6:483-95. 2004
    ..These studies demonstrate that mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate...
  92. pmc ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome
    Christophe Ginestier
    Department of Internal Medicine, Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA
    Cell Stem Cell 1:555-67. 2007
    ..These findings offer an important new tool for the study of normal and malignant breast stem cells and facilitate the clinical application of stem cell concepts...
  93. ncbi Generation of a functional mammary gland from a single stem cell
    Mark Shackleton
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
    Nature 439:84-8. 2006
    ..Our data establish that single cells within the Lin-CD29hiCD24+ population are multipotent and self-renewing, properties that define them as MaSCs...
  94. ncbi Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts
    Hiroshi Takayanagi
    Department of Immunology, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Japan
    Dev Cell 3:889-901. 2002
    ..Thus, NFATc1 may represent a master switch for regulating terminal differentiation of osteoclasts, functioning downstream of RANKL...
  95. ncbi Identification of a cancer stem cell in human brain tumors
    Sheila K Singh
    The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    Cancer Res 63:5821-8. 2003
    ..The identification of a BTSC provides a powerful tool to investigate the tumorigenic process in the central nervous system and to develop therapies targeted to the BTSC...
  96. pmc Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors
    Masaki Ieda
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, San Francisco, CA 94158, USA
    Cell 142:375-86. 2010
    ..Reprogramming of endogenous or explanted fibroblasts might provide a source of cardiomyocytes for regenerative approaches...
  97. pmc The epigenomics of cancer
    Peter A Jones
    Department of Urology, Biochemistry, and Molecular Biology, USC Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, USA
    Cell 128:683-92. 2007
    ....
  98. pmc A validated regulatory network for Th17 cell specification
    Maria Ciofani
    Molecular Pathogenesis Program, The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA
    Cell 151:289-303. 2012
    ..This interconnected network can be used to investigate new therapeutic approaches to manipulate Th17 functions in the setting of inflammatory disease...
  99. pmc The transcriptional and epigenomic foundations of ground state pluripotency
    Hendrik Marks
    Department of Molecular Biology, Faculty of Science, Radboud University, PO Box 9101, 6500 HB Nijmegen, The Netherlands
    Cell 149:590-604. 2012
    ..These findings suggest that transcriptional potentiation and a permissive chromatin context characterize the ground state and that exit from it may not require a metastable intermediate or multilineage priming...
  100. pmc Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation
    Robert J Johnston
    Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology LIAI, 9420 Athena Circle, La Jolla, CA 92037, USA
    Science 325:1006-10. 2009
    ..These findings demonstrate that T(FH) cells are required for proper B cell responses in vivo and that Bcl6 and Blimp-1 play central but opposing roles in T(FH) differentiation...
  101. ncbi Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell
    D Bonnet
    Department of Genetics, Research Institute, Hospital for Sick Children, University of Toronto, Ontario, Canada
    Nat Med 3:730-7. 1997
    ..The SL-ICs were able to differentiate in vivo into leukemic blasts, indicating that the leukemic clone is organized as a hierarchy...

Research Grants73

  1. Xenoestrogen regulation of Leydig cells
    BENSON TOKUNBO AKINGBEMI; Fiscal Year: 2010
    ..soy isoflavones act directly in the seminiferous epithelium will be investigated by analysis of Sertoli cell differentiation and maturation...
  2. MECHANISMS IN THE REGULATION OF SP-A GENE EXPRESSION
    Carole R Mendelson; Fiscal Year: 2013
    ..cells in concert with surfactant phospholipid synthesis and, thus, serves as an excellent marker of type II cell differentiation and surfactant production...
  3. Rac GTPase signaling during embryonic salivary gland branching morphogenesis
    Sharon Sequeira; Fiscal Year: 2012
    ..However, the considerable challenge of stimulating and/or maintaining salivary epithelial cell differentiation in artificially engineered tissues has been hindered by the lack of knowledge of precise molecular ..
  4. Cellular &Molecular Defects in Human B Cell Development
    Charlotte Cunningham-Rundles; Fiscal Year: 2013
    ..ligand interactor (TACI) and the function of its ligands and toll like receptor ligands (TLR) in B cell differentiation and maturation...
  5. MODULATION OF IFN ACTION VIA NOVEL REGULATORY FACTORS
    DHAN V KALVAKOLANU; Fiscal Year: 2013
    ..One such protein is CCAAT/Enhancer Binding protein-beta (C/EBP-2), a transcription factor known to regulate cell differentiation, energy metabolism, immune response, tumor growth and apoptosis...
  6. Regulation of myocardial growth and death by GSK-3
    Junichi Sadoshima; Fiscal Year: 2013
    ..will lead to a better understanding of the molecular mechanisms mediating HF, ischemic injury, and stem cell differentiation, which can be utilized to develop specific interventions to treat HF and ischemic heart disease, and ..
  7. Sirtuin/FOXO Signaling in the Regulation of Bone Mass
    Stavroula Kousteni; Fiscal Year: 2012
    ..FOXO1, one of the three FOXO homologs, regulates cell differentiation, promotes either cell survival or apoptosis;and also increases lifespan in model biologic systems...
  8. Studying the effects of vitamin D on FOXP3 and IL-17 expression in human CD4+ T c
    Insoo Kang; Fiscal Year: 2010
    ..CD4+ T cells, leading to the enhanced development of FOXP3+iTreg with a reciprocal suppression of Th17 cell differentiation and IL-17 production...
  9. Unbiased genome-wide screen to identify genes regulating mucous cell hyperplasia
    Jeffrey A Whitsett; Fiscal Year: 2010
    ..shRNA library screen to identify genes and associated gene networks inducing or inhibiting goblet cell differentiation and mucous hypersecretion, and 2) an extensive small molecule compound library screen to identify compounds ..
  10. Chemical modulators of Tet-family proteins
    Anjana Rao; Fiscal Year: 2010
    ..genomic DNA of undifferentiated ES cells but not differentiated cell types, and hmC levels diminish upon ES cell differentiation. (ii) TET1 is an MLL partner in acute myeloid and lymphoid leukemia (AML, ALL), and TET2 is implicated in ..
  11. VACCINE INDUCED IMMUNITY IN THE YOUNG AND AGED
    Rafi Ahmed; Fiscal Year: 2013
    ..focus our studies on CD8 T cells and examine the mechanisms that regulate human effector and memory CD8 T cell differentiation. The following specific aims are proposed to achieve our goals: 1) To identify transcription factors that ..
  12. 4th ASM Conference on Prokaryotic Cell Biology and Development
    Susan S Golden; Fiscal Year: 2012
    ..Because cell differentiation involves a reorganization of cell shape, division, metabolism and gene expression, the investigation of ..
  13. Regulation of vaccine-induced anti-fungal T17 cells
    MARCEL WUETHRICH; Fiscal Year: 2013
    ..pathway crosstalk between TLRs and Card9, and that TLRs collaborate with the mannose receptor to induce T17 cell differentiation. We provide strong preliminary data to support our hypotheses...
  14. Histone Chaperones in Angiogenesis
    Soumen Paul; Fiscal Year: 2012
    ..In addition, using mouse embryonic stem cell differentiation system and isolated endothelial cells, we will determine whether HIRA function differentially regulates ..
  15. A novel telomerase expressing lung fibroblast phenotype
    Sem H Phan; Fiscal Year: 2012
    ..additional functional significance beyond the mere maintenance of telomere length, including regulation of cell differentiation, gene expression and cell survival/apoptosis...
  16. BMP signaling in vertebrate development
    Ken W Y Cho; Fiscal Year: 2013
    ..of BMPs have diverged in evolution and include the control of gametogenesis, early embryonic development, cell differentiation/apoptosis, and tissue homeostasis in the adult...
  17. Knockdown of Gene Expression in Immune Cells in Humanized Mice
    Judy Lieberman; Fiscal Year: 2010
    ..in vivo, we will design CD4-AsiCs to knockdown FOXP3 and BCL6, transcription factors that direct CD4 T cell differentiation into suppressive Treg cells and antibody-promoting TFH cells, respectively...
  18. Epithelial differentiation in cornea
    Bogi Andersen; Fiscal Year: 2012
    ..The long term goal of our research is to understand how cornea epithelial cell differentiation is maintained...
  19. ROLE OF FORKHEAD PROTEINS IN INSULIN ACTION
    Domenico Accili; Fiscal Year: 2013
    ..We have shown that Foxo1 regulates cell differentiation by interacting with the Notch pathway...
  20. Epigenetic downregulation of the antibody response and inhibition of autoimmunity
    Paolo Casali; Fiscal Year: 2013
    ..B cell functions, such as class switch DNA recombination (CSR), somatic hypermutation (SHM) and plasma cell differentiation, thereby informing the antibody response...
  21. Regulation of Genomic and Epigenomic Stability at CpG Sites
    Alfonso Bellacosa; Fiscal Year: 2013
    ..is required for normal development using a knock-in strain and assess which TDG function is required for ES cell differentiation in vitro and for phenotypes in mouse embryo fibroblasts...
  22. Heat Shock Effects on Human Mesenchymal Stem Cell Differentiation in Hydrogel
    Sihong Wang; Fiscal Year: 2010
    ..However, the mechanisms for temperature-induced bone cell differentiation as well as overexpression of HSPs during embryonic development are unclear...
  23. Analysis of epigenetic regulation in early mammalian embryos via RNA interference
    Charles R Long; Fiscal Year: 2010
    ..provide the first evidence of the functional genes controlling the embryonic epigenome and their effects on cell differentiation in a mammalian species other than the mouse...
  24. The Regulation of Salivary Gland Regeneration by P2Y2 Nucleotide Receptors
    GARY ANDREW WEISMAN; Fiscal Year: 2013
    ..e., cell proliferation and migration) and organization of acini into a functional gland structure (i.e., cell differentiation into a polarized epithelium with high transepithelial resistance)...
  25. Fate of Lung Stem Cells During Pulmonary Disease
    Susan M Majka; Fiscal Year: 2012
    ..cell types and functions as well as a lack of understanding of how chronic disease processes affect stem cell differentiation. Therefore, prior to testing cell-based therapy, it is desirable to use a pre-clinical animal model of PAH ..
  26. The Effects of PP2A on TNF Signaling and Smoke-Induced Lung Injury
    Robert F Foronjy; Fiscal Year: 2013
    ..respond to environmental and nutritional stresses by regulating gene expression, cellular proliferation and cell differentiation. The cell contains a tightly coordinated network of kinases and phosphatases that switch proteins from the ..
  27. MECHANISMS OF GASTROINTESTINAL GROWTH AND TRANSFORMATION
    Juanita L Merchant; Fiscal Year: 2013
    ..pATMS1981-expressing cells in APCmin polyps were absent suggesting that excess Wnt signaling prevents EC cell differentiation. In the current application, three aims are proposed to test the overarching hypothesis that ZBP-89 ..
  28. Maternal Obesity affects AMP-Kinase in Muscle Cell Differentiation
    Min Du; Fiscal Year: 2013
    ....
  29. Regulated Accessibility of a cytokine gene locus.
    Anjana Rao; Fiscal Year: 2013
    ..and histone modification;explored the transcriptional and chromatin-based programs underlying CD8 T cell differentiation;and defined the functions of multiple regulatory regions in the Il4, Ifng and Il10 loci...
  30. MicroRNA Regulation of Macrophage Polarization in Muscle Regeneration
    Paula K Shireman; Fiscal Year: 2013
    ..are small, noncoding RNAs that inhibit gene expression, thereby regulating many processes, including immune cell differentiation. A better understanding of the mechanisms of skeletal muscle regeneration and skin wound healing, and the ..
  31. Role of natural indoles in experimental autoimmune encephalomyelitis
    Mitzi Nagarkatti; Fiscal Year: 2013
    ..Also, recent studies suggested that AhR may also regulate certain important immune functions such as T cell differentiation. We have made an exciting observation that dietary indoles such as DIM can completely suppress the clinical ..
  32. DNA Methylation Control Hematopoietic Stem Cell Lineage Differentiation
    GRANT ANTHONY CHALLEN; Fiscal Year: 2013
    ..first two years of my post-doc, during which I have studied the molecular regulation of hematopoietic stem cell differentiation and the epigenetic mechanisms that control stem cell fate...
  33. Inflammatory hyperalgesia due to TRPV1, the pepper spray receptor in the cornea
    Sharona E Gordon; Fiscal Year: 2013
    ..players and their interactions in sensitization of TRPV1 may shed light on a signaling pathway essential to cell differentiation, metabolism, and survival in addition to leading to an understanding of TRPV1 regulation important for ..
  34. Molecular physiology of voltage-gated Ca2+ channels
    Jian Yang; Fiscal Year: 2013
    ..processes, ranging from muscle contraction, heartbeat, neural communication and hormone secretion to cell differentiation, motility, growth and death...
  35. CD4+ T cells in Invasive Aspergillosis
    Amariliz Rivera; Fiscal Year: 2012
    ..dissected the specific contributions of innate cells and innate receptors in shaping fungus-specific CD4 T cell differentiation in response to a primary, pulmonary infection with A.fumigatus spores...
  36. Effect of Neonatal Hyperoxia on Alveolar Development and Infection
    MICHAEL A O'REILLY; Fiscal Year: 2012
    ..By defining how hyperoxia affects alveolar epithelial cell differentiation, we hope to clarify how it disrupts neonatal lung development and why infants born prematurely continue to ..
  37. Role in Myopia Development of Retinal Pigment Epithelium - A New Therapeutic Targ
    Yan Zhang; Fiscal Year: 2013
    ..Luo (UC Berkeley), whose research focus is the TGF-[unreadable] family including BMPs and their roles in cell differentiation, tissue morphogenesis, and extracellular matrix production...
  38. Identification of Mechanoresponsive Promoter Elements in Chondrogenesis
    Dominik R Haudenschild; Fiscal Year: 2013
    ..responses that are critical to cartilage ma trix composition, cellular survival, and progenitor cell differentiation. Cellular responses to me- chanical forces vary greatly, and can range from proliferation, to ..
  39. Functional characterization of the Bronx waltzer deafness gene
    Botond Banfi; Fiscal Year: 2013
    ..gene that regulates essential RNA splicing in the cochlea and vestibular system will lead to the discovery of novel molecular mechanisms, proteins, and protein isoforms that are required for hair cell differentiation and survival.
  40. In utero programming of the dopamine system: behavior, neuroanatomy &epigenetics
    Teresa M Reyes; Fiscal Year: 2013
    ..hypomethylation and increased expression of CDKN1c in IUGR animals, a gene critical for dopaminergic cell differentiation, which may alter the developmental trajectory of dopaminergic neurons...
  41. Perinatal Programming of Infant Stress Reactivity and the Atopic Phenotype
    Rosalind J Wright; Fiscal Year: 2013
    ..atopic disorders (early sensitization as indexed by IgE expression and skin test reactivity, T-helper cell differentiation, airway resistance, early clinical phenotypes)...
  42. Chemical approaches for studying the biology of CD38
    Hening Lin; Fiscal Year: 2013
    ..PUBLIC HEALTH RELEVANCE: CD38 is important for immune cell differentiation and function, insulin secretion, and neuropeptide release...
  43. Lung and bone marrow crosstalk during a respiratory infection
    CAROLINA B LOPEZ; Fiscal Year: 2010
    ..that are highly elevated in the blood of animals undergoing a lung infection have been associated with cell differentiation and release from the BM...
  44. Transcriptional regulation of enteroendocrine cell differentiation by NeuroD
    Andrew B Leiter; Fiscal Year: 2013
    ..Genome-wide chromatin occupancy studies (ChIPseq) will identify a subset of differentially expressed genes as potential direct targets by NeuroD. ..
  45. AKT Signaling and Oxidative Stress Regulation of Erythropoiesis
    Saghi Ghaffari; Fiscal Year: 2013
    ..Elucidating molecular pathways that regulate erythroid cell differentiation and maturation is critical for devising targeted treatments for a number of erythroid disorders including ..
  46. REGULATION OF ATROPHY-INDUCED PROGENITOR CELLS IN THE GASTRIC CORPUS
    Jason C Mills; Fiscal Year: 2013
    ..g., hematopoietic stem cell differentiation changes in arthritis)...
  47. A Fourth Outcome: DNA Damage and the Differentiation of B Cells
    Michael A Teitell; Fiscal Year: 2013
    ..These pathway alterations are predicted to result in increased GC B cell proliferation and impaired plasma cell differentiation, which will be tested here in vitro and in vivo...
  48. The Forkhead Transcription Factor, FOXO1, and its Role in Pituitary Gland Develop
    BUFFY SUE ELLSWORTH; Fiscal Year: 2010
    ..in the pituitary gland during development suggests a role for inhibiting cell division and regulating cell differentiation. Our objective is to determine the requirement of FOXO1 for normal pituitary development by analyzing the ..
  49. The role of the nucleoporin Nup98 in gene regulation
    Martin W Hetzer; Fiscal Year: 2013
    ..The precise execution of transcriptional programs during cell differentiation and development relies on faithful reproduction of a specific chromatin state through mitosis...
  50. Regulation of Organized Intestinal Lymphoid Tissues by TRANCE/RANKL
    Ifor R Williams; Fiscal Year: 2012
    ..acting through its receptor RANK on epithelial cells is a critical signaling pathway required for normal M cell differentiation and function...
  51. Enzymology of Post-translational Modifications
    Carol A Fierke; Fiscal Year: 2013
    ..Acetylation affects multiple cellular processes, including gene expression and cell differentiation. All of these enzymes are current targets for drug development for a variety of diseases;HDAC inhibitors ..
  52. Cell Specific Expression in the Pituitary Gland
    Sally A Camper; Fiscal Year: 2013
    ..regulate the transition from cell proliferation to differentiation and by understanding the process of stem cell differentiation into hormone producing cells...
  53. EGF RECEPTOR MEDIATED SIGNALING IN DROSOPHILA
    GERTRUD M SCHUPBACH; Fiscal Year: 2013
    ..In particular, we have isolated mutations that affect posterior follicle cell differentiation and uncover interactions of Egfr signaling with the Notch pathway...
  54. PTIP as an epigenetic regulator in male meiosis
    Guo Zhang Zhu; Fiscal Year: 2013
    ..of the germ cell epigenome during spermatogenesis and broadly define an epigenetic mechanism of cell differentiation and development...
  55. Regulation and Function of the Rhox8 homeobox gene in granulosa cells
    JAMES ARTHUR MACLEAN; Fiscal Year: 2010
    ..expressed through the periovulatory window, suggesting it may be uniquely situated to regulate luteal cell differentiation and the final steps of ovulation...
  56. THE FUNCTION AND REGULATION OF HISTIDINE DECARBOXYLASE IN GUT INFLAMMATION
    TIMOTHY CRAGIN WANG; Fiscal Year: 2013
    ..Taken together, these data indicate key roles for HDC/histamine in myeloid cell differentiation, and CD11b+Ly6G+ IMCs in early colon cancer development...
  57. Diet and Alcohol Induced Epigenetic Changes in Oral Cavity Carcinogenesis Model
    Lorraine J Gudas; Fiscal Year: 2013
    ..shown that RA is a signaling molecule which has a major role in initiating epigenetic changes during stem cell differentiation. The specific aims of the application are: (1) to measure the effects of alcohol on the incidence of oral ..
  58. Merkel cells specify innervating SAI sensory neuron phenotype.
    Erin G Reed-Geaghan; Fiscal Year: 2013
    ..Cutaneous SAI innervation develops independently of Merkel cell differentiation, and is maintained in the absence of Merkel cells...
  59. Role of SATB in placental development
    Mohammad A K Rumi; Fiscal Year: 2013
    ..remodeling and epigenetic changes play a fundamental role in the regulation of gene expression during cell differentiation. Special AT-rich sequence binding protein 1 (SATB1) and SATB2 act as chromatin organizers as well as ..
  60. Human Pluripotent Stem Cell Differentiation with Defined O2 & Protein Engagement
    ROBERT SAMUEL LANGER; Fiscal Year: 2010
    ..For example, this can lead to ways to grow functioning heart muscle cells that can be used to treat heart disease. ..
  61. Calcification and Vascular Cell Differentiation
    Cecilia M Giachelli; Fiscal Year: 2013
    ....
  62. Role of Gsalpha in Regulating Osteoblast Differentiation
    JOY Y WU; Fiscal Year: 2012
    ..Finally, Aim 3 will focus on determining the ability of osteoprogenitors to differentiate towards other mesenchymal lineages such as adipocytes, using lineage tracing in vivo and cell differentiation assays in vitro.
  63. SEGMENTATION AND PATTERNING IN DROSOPHILA
    Eric Wieschaus; Fiscal Year: 2000
    ..The goal of the experiments proposed in this application is to understand how complex patterns of cell differentiation are established in epithelial cells during Drosophila embryonic development...
  64. JAK-3 AND MYELOID CELL DIFFERENTIATION
    E Reddy; Fiscal Year: 2000
    ..of JAK3 is due to new transcription or post-transcriptional mechanisms; 3) to determine how myeloid cell differentiation is affected when JAK-3 expression is inhibited using anti-sense oligonucleotides; in addition, preliminary ..
  65. NR4A Regulation of Organ Morphogenesis
    CHRISTOPHER GISSENDANNER; Fiscal Year: 2009
    ..are a conserved, functionally diverse group of transcription factors that regulate cell proliferation, cell differentiation, and apoptosis in a variety of developmental and physiological contexts...
  66. IMMUNOBIOLOGY OF B CELL DIFFERENTIATION
    Kenneth Dorshkind; Fiscal Year: 1991
    ..One, termed S17, supports B cell differentiation while another, S10, does not...
  67. Genetic Analysis of Mammalian CNS Development
    E Crenshaw; Fiscal Year: 2004
    ..have been proposed for BMP signaling during neural development, including dorsal/ventral patterning, glial cell differentiation, growth and differentiation of peripheral neurons, and neuronogenesis during cortical development...
  68. Critical Role of Mybl2 in Spontaneous and Short-Chain Fatty Acid-Induced Intestin
    MICHAEL JOSEPH PAPETTI; Fiscal Year: 2011
    DESCRIPTION (from the applicant): Colonic epithelial cell differentiation is driven by reprogramming of stem and/or progenitor cells as they migrate upward along the crypt axis...
  69. Function of Nkx homeobox genes in enteroendocrine cell differentiation
    TRACY EDIGER; Fiscal Year: 2009
    ..2 and Nkx6.3 in gastric endocrine cell differentiation and to define the lineage pathway through which Nkx6.3 specifies G cells...
  70. CYTOKINES AND NK CELL DIFFERENTIATION IN RODENT DECIDUA
    Judith Head; Fiscal Year: 1999
    ..In Aim 1, decidual tissue, taken during the time of NK cell differentiation, and metrial gland tissue will be surveyed for the presence of cytokines which can influence NK cells, ..
  71. Vitamin D in the Cornea and Anterior Segment of the Eye
    Mitchell A Watsky; Fiscal Year: 2010
    ..increasing attention in health-related studies due to their role in numerous cellular functions, including cell differentiation and matrix turnover...
  72. NOTCH SIGNALING REGULATES GRANULOSA CELL DIFFERENTIATION
    NORMA WILSON RAWLS; Fiscal Year: 2002
    ..The goal of this pplication is to examine the role of the Notch pathway in granulosa cell differentiation. Initial work will determine he spatial and temporal pattern of expression of components of the Notch ..
  73. PROKARYOTIC SWARM CELL DIFFERENTIATION & PHOTOPERCEPTION
    Carl Bauer; Fiscal Year: 2001
    DESCRIPTION (Adapted from the applicants abstract): This proposal is centered on an investigation of swarm cell differentiation and photoperception by the bacterium Rhodospirillum centenum...