Summary: The chromosomal constitution of cells, in which each type of CHROMOSOME is represented twice. Symbol: 2N or 2X.

Top Publications

  1. pmc Chromosome triplication found across the tribe Brassiceae
    Martin A Lysak
    Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3AB, United Kingdom
    Genome Res 15:516-25. 2005
  2. ncbi Comprehensive mass-spectrometry-based proteome quantification of haploid versus diploid yeast
    Lyris M F de Godoy
    Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nature 455:1251-4. 2008
  3. ncbi Unravelling angiosperm genome evolution by phylogenetic analysis of chromosomal duplication events
    John E Bowers
    Plant Genome Mapping Laboratory, University of Georgia, Athens, Georgia 30602, USA
    Nature 422:433-8. 2003
  4. pmc The diploid genome sequence of an Asian individual
    Jun Wang
    Beijing Genomics Institute at Shenzhen, Shenzhen 518000, China
    Nature 456:60-5. 2008
  5. ncbi UVB-induced premature senescence of human diploid skin fibroblasts
    Florence Chainiaux
    Laboratory of Cellular Biochemistry and Biology, Department of Biology, University of Namur FUNDP, Rue de Bruxelles 61, B 5000, Namur, Belgium
    Int J Biochem Cell Biol 34:1331-9. 2002
  6. ncbi Sexual reproduction between partners of the same mating type in Cryptococcus neoformans
    Xiaorong Lin
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nature 434:1017-21. 2005
  7. pmc The parasexual cycle in Candida albicans provides an alternative pathway to meiosis for the formation of recombinant strains
    Anja Forche
    Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America
    PLoS Biol 6:e110. 2008
  8. ncbi Genome-wide genetic analysis of polyploidy in yeast
    Zuzana Storchova
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 443:541-7. 2006
  9. pmc Mechanistic plasticity of sexual reproduction and meiosis in the Candida pathogenic species complex
    Jennifer L Reedy
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 19:891-9. 2009
  10. pmc Turning meiosis into mitosis
    Isabelle d'Erfurth
    INRA, UR254, IJPB, Versailles France
    PLoS Biol 7:e1000124. 2009

Detail Information

Publications326 found, 100 shown here

  1. pmc Chromosome triplication found across the tribe Brassiceae
    Martin A Lysak
    Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3AB, United Kingdom
    Genome Res 15:516-25. 2005
    ..The presumed hexaploidization event occurred after the Arabidopsis-Brassiceae split, between 7.9 and 14.6 Mya...
  2. ncbi Comprehensive mass-spectrometry-based proteome quantification of haploid versus diploid yeast
    Lyris M F de Godoy
    Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nature 455:1251-4. 2008
    ..Systems-wide, precise quantification directly at the protein level opens up new perspectives in post-genomics and systems biology...
  3. ncbi Unravelling angiosperm genome evolution by phylogenetic analysis of chromosomal duplication events
    John E Bowers
    Plant Genome Mapping Laboratory, University of Georgia, Athens, Georgia 30602, USA
    Nature 422:433-8. 2003
  4. pmc The diploid genome sequence of an Asian individual
    Jun Wang
    Beijing Genomics Institute at Shenzhen, Shenzhen 518000, China
    Nature 456:60-5. 2008
    ..These variations were considered for their potential biological impact. Our sequence data and analyses demonstrate the potential usefulness of next-generation sequencing technologies for personal genomics...
  5. ncbi UVB-induced premature senescence of human diploid skin fibroblasts
    Florence Chainiaux
    Laboratory of Cellular Biochemistry and Biology, Department of Biology, University of Namur FUNDP, Rue de Bruxelles 61, B 5000, Namur, Belgium
    Int J Biochem Cell Biol 34:1331-9. 2002
    ..This model could be used to test whether HDFs in UVB-induced premature senescence are able to promote epithelial cell growth and tumorigenesis in skin, as shown recently with HDFs in H(2)O(2)-induced premature senescence...
  6. ncbi Sexual reproduction between partners of the same mating type in Cryptococcus neoformans
    Xiaorong Lin
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nature 434:1017-21. 2005
  7. pmc The parasexual cycle in Candida albicans provides an alternative pathway to meiosis for the formation of recombinant strains
    Anja Forche
    Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America
    PLoS Biol 6:e110. 2008
    ..albicans. We discuss, in light of the long association of C. albicans with warm-blooded animals, the potential advantages of a parasexual cycle over a conventional sexual cycle...
  8. ncbi Genome-wide genetic analysis of polyploidy in yeast
    Zuzana Storchova
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 443:541-7. 2006
    ..Thus, geometric constraints may have profound effects on genome stability; the phenomenon described here may be relevant in a variety of biological contexts, including disease states such as cancer...
  9. pmc Mechanistic plasticity of sexual reproduction and meiosis in the Candida pathogenic species complex
    Jennifer L Reedy
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 19:891-9. 2009
  10. pmc Turning meiosis into mitosis
    Isabelle d'Erfurth
    INRA, UR254, IJPB, Versailles France
    PLoS Biol 7:e1000124. 2009
    ..The obtained plants produce functional diploid gametes that are genetically identical to their mother. The creation of the MiMe genotype and apomeiosis phenotype is an important step towards understanding and engineering apomixis...
  11. pmc Cryptic fitness advantage: diploids invade haploid populations despite lacking any apparent advantage as measured by standard fitness assays
    Aleeza C Gerstein
    Zoology Department, Biodiversity Research Center, The University of British Columbia, Vancouver, British Columbia, Canada
    PLoS ONE 6:e26599. 2011
    ..none of our fitness component measures (lag phase, growth rate, biomass production) indicated an advantage to diploidy. Similarly, competition assays against a common competitor and direct competition between haploid and diploid ..
  12. pmc A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae
    L Ni
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Mol Biol Cell 12:2147-70. 2001
    ..Genome-wide screens of defined collections of mutants hold significant promise for dissecting many biological processes in yeast...
  13. pmc Transmission genetics of chromatin from a synthetic amphidiploid to cultivated peanut (Arachis hypogaea L.). broadening the gene pool of a monophyletic polyploid species
    M D Burow
    Department of Crop and Soil Science, University of Georgia, Athens, 30602, USA
    Genetics 159:823-37. 2001
    ..Unexpectedly, 68% of the markers deviating from expected segregation showed an excess of the synthetic parent allele. Genetic consequences, relationship to species origins, and significance for comparative genetics are discussed...
  14. ncbi Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth
    R L Roberts
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge
    Genes Dev 8:2974-85. 1994
    ..Our results provide a novel developmental model to study the dynamics of signal transduction, with implications for higher eukaryotes...
  15. ncbi Homoeologous nonreciprocal recombination in polyploid cotton
    Armel Salmon
    Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA 50011, USA
    New Phytol 186:123-34. 2010
    ..In addition, some genomic regions show multiple patterns of homoeologous recombination among species...
  16. ncbi Parent-of-origin effects on seed development in Arabidopsis thaliana
    R J Scott
    Department of Biology, University of Leicester, University Road, Leicester LE1 7RH, UK
    Development 125:3329-41. 1998
    ..One explanation of our observations is that seeds with maternal or paternal excess contain different doses of maternally or paternally expressed imprinted loci affecting endosperm development...
  17. ncbi Genetic regulation of meiosis in polyploid species: new insights into an old question
    Marta Cifuentes
    Institut Jean Pierre Bourgin, Station de Génétique et Amélioration des Plantes, 78026 Versailles Cedex, France
    New Phytol 186:29-36. 2010
    ..Some of these advances are discussed, and it is suggested that research on polyploidy and on meiosis should no longer be unlinked...
  18. pmc From the Cover: mitotic gene conversion events induced in G1-synchronized yeast cells by gamma rays are similar to spontaneous conversion events
    Phoebe S Lee
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 107:7383-8. 2010
  19. pmc Gamma-tocotrienol modulation of senescence-associated gene expression prevents cellular aging in human diploid fibroblasts
    Suzana Makpol
    Universiti Kebangsaan Malaysia, Faculty of Medicine, Department of Biochemistry, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia
    Clinics (Sao Paulo) 67:135-43. 2012
    ..This study determined the molecular mechanism of γ-tocotrienol, a vitamin E homolog, in the prevention of cellular aging in human diploid fibroblasts using the expression of senescence-associated genes...
  20. ncbi Chromosome transfer induced aneuploidy results in complex dysregulation of the cellular transcriptome in immortalized and cancer cells
    Madhvi B Upender
    Genetics Branch and Laboratory for Biosystems and Cancer, Center for Cancer Research and Biometric Research Branch, National Cancer Institute NIH, Bethesda, Maryland 20892 8010, USA
    Cancer Res 64:6941-9. 2004
    ..We therefore postulate that the genomic imbalances observed in cancer cells exert their effect through a complex pattern of transcriptional dysregulation...
  21. ncbi Distant hybridization leads to different ploidy fishes
    Shaojun Liu
    Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China
    Sci China Life Sci 53:416-25. 2010
    ..The results indicated distant hybridization or the combination of this method with gynogenesis or androgenesis affects the formation of different ploidy fish with genetic variation...
  22. pmc Karyotype diversification and evolution in diploid and polyploid South American Hypochaeris (Asteraceae) inferred from rDNA localization and genetic fingerprint data
    Hanna Weiss-Schneeweiss
    Department of Systematic and Evolutionary Botany, University of Vienna, Rennweg 14, A 1030 Vienna, Austria
    Ann Bot 101:909-18. 2008
    ..The aim of this paper is to assess directions and mechanisms of karyotype evolution in South American species by interpreting both newly obtained and previous data concerning rDNA localization in a phylogenetic context...
  23. pmc Fast diploidization in close mesopolyploid relatives of Arabidopsis
    Terezie Mandáková
    Department of Functional Genomics and Proteomics, Institute of Experimental Biology, Masaryk University, Kamenice 5, CZ 625 00 Brno, Czech Republic
    Plant Cell 22:2277-90. 2010
    ..Our results underline the significance of multiple rounds of WGD in the angiosperm genome evolution and demonstrate that chromosome number per se is not a reliable indicator of ploidy level...
  24. pmc Nuclear and plastid haplotypes suggest rapid diploid and polyploid speciation in the N Hemisphere Achillea millefolium complex (Asteraceae)
    Yan Ping Guo
    Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, and College of Life Sciences, Beijing Normal University, Beijing 100875, China
    BMC Evol Biol 12:2. 2012
  25. ncbi Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species
    Z Liu
    The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Chinese Academy of Sciences, Beijing 100101, China
    Theor Appl Genet 106:1368-78. 2003
    ..Consequently, HMW glutenin subunits from the two diploid species may have potential value in improving the processing properties of hexaploid wheat varieties...
  26. ncbi Tracking the evolutionary history of polyploidy in Fragaria L. (strawberry): new insights from phylogenetic analyses of low-copy nuclear genes
    M Rousseau-Gueutin
    INRA, UR 419, Unité de Recherche sur les Espèces Fruitières, Domaine de la Grande Ferrade, Villenave D Ornon, France
    Mol Phylogenet Evol 51:515-30. 2009
    ..Additionally, sequence analyses also revealed relaxed constraints on homoeologous copies at high ploidy level, as demonstrated by deletion events within DHAR coding sequences of some allo-octoploid haplotypes...
  27. pmc Ploidy dictates repair pathway choice under DNA replication stress
    Xin Chenglin Li
    Department of Molecular Biology and Genetics, College of Agriculture and Life Sciences, Cornell University, Ithaca, New York 14853, USA
    Genetics 187:1031-40. 2011
    ..In response to replication stress, early events associated with ploidy dictate the repair pathway choice. This study uncovers a fundamental difference between haplophase and diplophase in the maintenance of genome integrity...
  28. ncbi Large changes in anatomy and physiology between diploid Rangpur lime (Citrus limonia) and its autotetraploid are not associated with large changes in leaf gene expression
    Thierry Allario
    Centre de Coopération Internationale en Recherche Agronomique pour Dévelopement, UPR amélioration génétique des espèces à multiplication végétative, Instituto Valenciano de Investigaciones Agrarias, E 46113 Moncada Valencia, Spain
    J Exp Bot 62:2507-19. 2011
    ..This suggests that, in 4x Rangpur lime, subtle changes in gene expression may be at the origin of the phenotypic differentiation of 4x citrus when compared with 2x...
  29. ncbi Genomic expression dominance in the natural allopolyploid Coffea arabica is massively affected by growth temperature
    Amélie Bardil
    CIRAD, UMR RPB, équipe DIVA, Montpellier, France
    New Phytol 192:760-74. 2011
    ..Our data revealed evidence of transcription profile divergence between the allopolyploid and its parental species, greatly affected by environmental conditions, and provide clues to the plasticity phenomenon in allopolyploids...
  30. pmc Evolution and expression of homeologous loci in Tragopogon miscellus (Asteraceae), a recent and reciprocally formed allopolyploid
    Jennifer A Tate
    Department of Botany, University of Florida, Gainesville, Florida 32611, USA
    Genetics 173:1599-611. 2006
    ..Synthetic F(1) hybrids between putative diploid progenitors are additive of their parental genomes, suggesting that polyploidization rather than hybridization induces genomic changes in Tragopogon...
  31. ncbi Molecular dissection of hyperdiploid multiple myeloma by gene expression profiling
    Wee J Chng
    Department of Hematology Oncology, Mayo Clinic, Scottsdale, Arizona, USA
    Cancer Res 67:2982-9. 2007
    ..Furthermore, the signatures that defined these clusters may provide a basis for tailoring treatment to individual patients...
  32. ncbi Differential sensitivity of the Arabidopsis thaliana transcriptome and enhancers to the effects of genome doubling
    Daniela Pignatta
    Department of Plant Biology and Genome Center, University of California, Davis, CA 95616, USA
    New Phytol 186:194-206. 2010
    ..The unique sensitivity of enhancer traps to ploidy, in contrast to the observed stability of genes, could derive from lower complexity of regulatory pathways acting on traps versus endogenous genes...
  33. ncbi Immortalization of human small airway epithelial cells by ectopic expression of telomerase
    Chang Q Piao
    Center for Radiological Research, College of Physicians and Surgens, Mailman School of Public Health, Columbia University, New York, NY 10032, USA
    Carcinogenesis 26:725-31. 2005
  34. pmc Chromosome rearrangements and aneuploidy in yeast strains lacking both Tel1p and Mec1p reflect deficiencies in two different mechanisms
    Jennifer L McCulley
    Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 107:11465-70. 2010
    ..Restoration of wild-type telomere lengths in the tel1 mec1 strain substantially reduced the rate of chromosome rearrangements but had no effect on the frequency of aneuploidy...
  35. ncbi Comparative genomics and repetitive sequence divergence in the species of diploid Nicotiana section Alatae
    K Yoong Lim
    School of Biological and Chemical Sciences, Queen Mary University of London, London E1 4NS, UK
    Plant J 48:907-19. 2006
    ..This rapid rate of divergence is analogous to that found in polyploid species, and is therefore, in plants, not only associated with polyploidy...
  36. ncbi Allopolyploidy alters gene expression in the highly stable hexaploid wheat
    Ping He
    Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA
    Plant Mol Biol 52:401-14. 2003
    ..These genes were often altered non-randomly in different synthetic hexaploids as well as natural hexaploid wheat, suggesting that many of the gene expression changes were intrinsically associated with polyploidy...
  37. pmc Comparative genome-wide screening identifies a conserved doxorubicin repair network that is diploid specific in Saccharomyces cerevisiae
    Tammy J Westmoreland
    Department of Surgical Sciences, Duke University Medical Center, Durham, North Carolina, United States of America
    PLoS ONE 4:e5830. 2009
  38. ncbi Tissue-specific silencing of homoeologs in natural populations of the recent allopolyploid Tragopogon mirus
    Richard J A Buggs
    Department of Biology, University of Florida, Gainesville, FL 32611, USA
    New Phytol 186:175-83. 2010
    ..Our study shows that tissue-specific silencing, and even apparent subfunctionalization, can arise rapidly in the early generations of natural allopolyploidy...
  39. pmc Rapid concerted evolution of nuclear ribosomal DNA in two Tragopogon allopolyploids of recent and recurrent origin
    A Kovarik
    Institute of Biophysics, Academy of Sciences of the Czech Republic, CS 61265 Brno, Czech Republic
    Genetics 169:931-44. 2005
    ..dubius, the rDNA of T. dubius was nonetheless expressed. We have apparently caught homogenization of rDNA repeats (concerted evolution) in the act, although it has not proceeded to completion in any allopolyploid population yet examined...
  40. ncbi Towards a unified genetic map for diploid roses
    Monika Spiller
    Institute for Plant Genetics, Leibniz University Hannover, Herrenhaeuser Strasse 2, Hannover, Germany
    Theor Appl Genet 122:489-500. 2011
    ..It will benefit future efforts of the rose community to sequence the whole rose genome and will be useful for synteny studies in the Rosaceae family and especially in the section Rosoideae...
  41. pmc Mnd1/Hop2 facilitates Dmc1-dependent interhomolog crossover formation in meiosis of budding yeast
    Jill M Henry
    The Stowers Institute for Medical Research, 1000 E 50th St, Kansas City, MO 64110, USA
    Mol Cell Biol 26:2913-23. 2006
    ..Our results support a model in which Mnd1/Hop2 is required for DNA-DNA interactions that help ensure Dmc1-mediated stable strand invasion between homologous chromosomes, thereby preserving genomic integrity...
  42. ncbi Effective population size in Hymenoptera with complementary sex determination
    A Zayed
    Department of Biology, York University, 4700 Keele St, Toronto, Ontario, M3J 1P3, Canada
    Heredity (Edinb) 93:627-30. 2004
    ..In both models, low allelic diversity at the sex locus reduces the Ne of hymenopteran populations, and this effect is largest when diploid males are effectively sterile...
  43. pmc Diploid genome reconstruction of Ciona intestinalis and comparative analysis with Ciona savignyi
    Jong Hyun Kim
    Department of Computer Science, Yonsei University, Seoul, Republic of Korea
    Genome Res 17:1101-10. 2007
    ..Our results indicate 97% accuracy in haplotype estimation, based on a simulated data set. We conduct a comparative analysis with Ciona savignyi, and discover interesting patterns of conserved DNA elements in chordates...
  44. pmc The diploid genome sequence of an individual human
    Samuel Levy
    J Craig Venter Institute, Rockville, Maryland, USA
    PLoS Biol 5:e254. 2007
    ..These data depict a definitive molecular portrait of a diploid human genome that provides a starting point for future genome comparisons and enables an era of individualized genomic information...
  45. ncbi Irreversible cellular senescence induced by prolonged exposure to H2O2 involves DNA-damage-and-repair genes and telomere shortening
    Jianming Duan
    Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100083, China
    Int J Biochem Cell Biol 37:1407-20. 2005
    ..Our findings indicate that several aging theories can be merged together by a common mechanism of oxidative damage, and that the level of oxidative DNA-damage-and-repair capacity may be exploited as reliable markers of cell senescence...
  46. ncbi The development of a bin mapping population and the selective mapping of 103 markers in the diploid Fragaria reference map
    D J Sargent
    East Malling Research, New Road, East Malling, Kent ME19 6BJ, UK
    Genome 51:120-7. 2008
  47. ncbi Outbreeding depression, but no inbreeding depression in haplodiploid Ambrosia beetles with regular sibling mating
    Katharina Peer
    Department of Behavioural Ecology, Institute of Zoology, University of Bern, Wohlenstrasse 50a, CH 3032 Hinterkappelen, Switzerland
    Evolution 59:317-23. 2005
    ..The causes and consequences of our findings are discussed with respect to mating strategies, sex ratios, and speciation in this unusual system...
  48. ncbi Autopolyploidy differentially influences body size in plants, but facilitates enhanced accumulation of secondary metabolites, causing increased cytosine methylation
    Umesh C Lavania
    CSIR Central Institute of Medicinal and Aromatic Plants, PO CIMAP, Lucknow 226 015, India
    Plant J 71:539-49. 2012
  49. pmc A generic estimation of population subdivision using distances between alleles with special reference for microsatellite loci
    Y Michalakis
    Laboratoire d Ecologie, Universite P and M Curie CNRS URA 258, Paris, France
    Genetics 142:1061-4. 1996
    ..We discuss the relationships among these estimators and show how a single analysis of variance framework can accomodate these qualitatively different data types...
  50. pmc Function of Cryptococcus neoformans KAR7 (SEC66) in karyogamy during unisexual and opposite-sex mating
    Soo Chan Lee
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, USA
    Eukaryot Cell 11:783-94. 2012
    ..neoformans mating, which demonstrates a different evolutionary trajectory for the KAR genes in C. neoformans compared to those in S. cerevisiae...
  51. pmc The "Cheshire Cat" escape strategy of the coccolithophore Emiliania huxleyi in response to viral infection
    Miguel Frada
    Equipe EPPO Evolution du Plancton et PaléoOcéans, Centre National de la Recherche Scientifique et Université Pierre et Marie Curie Unité Mixte de Recherche 7144, Station Biologique, 29682 Roscoff, France
    Proc Natl Acad Sci U S A 105:15944-9. 2008
    ..In E. huxleyi, this phenomenon can account for the fact that the selective balance is tilted toward the boom-and-bust scenario of optimization of both growth rates of calcifying E. huxleyi cells and infectivity of EhVs...
  52. pmc Independent, rapid and targeted loss of highly repetitive DNA in natural and synthetic allopolyploids of Nicotiana tabacum
    Simon Renny-Byfield
    School of Biological and Chemical Sciences, Queen Mary University of London, London, United Kingdom
    PLoS ONE 7:e36963. 2012
    ..This occurs across multiple blocks of discontinuous repeats and based on the distribution of nucleotide similarity among NicCL3 units, was concurrent with rounds of sequence homogenisation...
  53. pmc Drosophila myb is required for the G2/M transition and maintenance of diploidy
    A L Katzen
    Department of Molecular Genetics, University of Illinois at Chicago, College of Medicine, Chicago, Illinois 60607 7170, USA
    Genes Dev 12:831-43. 1998
    ..We conclude that Dm myb is required for completion of cell division and may serve two independent functions: promotion of mitosis, on the one hand, and prevention of endoreduplication when cells are arrested in G2, on the other...
  54. ncbi Transcriptional differences between triploid and diploid Chinook salmon (Oncorhynchus tshawytscha) during live Vibrio anguillarum challenge
    B Ching
    Department of Biological Sciences and Great Lakes Institute for Environmental Research, University of Windsor, Windsor, Ontario, Canada
    Heredity (Edinb) 104:224-34. 2010
  55. ncbi Tetraploid Artemisia annua hairy roots produce more artemisinin than diploids
    L De Jesus-Gonzalez
    Department of Biology and Biotechnology, Worcester Polytechnic Institute, 100 Institute Rd, Worcester, MA 01609, USA
    Plant Cell Rep 21:809-13. 2003
    ..This study provides an initial step in increasing our understanding of the role of polyploidy in secondary metabolite production, especially in hairy roots...
  56. pmc Coexistence of diploid and triploid hybrid water frogs: population differences persist in the apparent absence of differential survival
    Ditte G Christiansen
    Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Winterthurerstrasse 190, Zurich, Switzerland
    BMC Ecol 10:14. 2010
    ..These populations, in which the parental species (P. lessonae with genotype LL and P. ridibundus with genotype RR) are absent, have pond-specific proportions of diploid (LR) and triploid (LLR and LRR) genotypes...
  57. pmc The fate of transposable elements in asexual populations
    Elie S Dolgin
    Institute of Evolutionary Biology, University of Edinburgh, Edinburgh EH9 3JT, United Kingdom
    Genetics 174:817-27. 2006
    ..These results may have important consequences for newly arisen asexual lineages and may account for the lack of deleterious retrotransposons in the putatively ancient asexual bdelloid rotifers...
  58. ncbi Array-CGH analysis of microsatellite-stable, near-diploid bowel cancers and comparison with other types of colorectal carcinoma
    Angela M Jones
    Molecular and Population Genetics Laboratory, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Oncogene 24:118-29. 2005
    ..In conclusion, our data support the suggestion that some MSI-CIN- carcinomas form a qualitatively different group from the other cancer types, and also suggest that the MSI-CIN- group is itself heterogeneous...
  59. ncbi Genomic in situ hybridization (GISH) discriminates between the A and the B genomes in diploid and tetraploid Setaria species
    A Benabdelmouna
    , Institut National de la Recherche Agronomique, BP 86510, Dijon, France
    Genome 44:685-90. 2001
    ..Only one polyploid species, S. pumila, was shown to bear an unknown genomic composition that is not closely related either to genome A or to genome B...
  60. ncbi PCR-based assay for mating type and diploidy in Chlamydomonas
    Ivan Zamora
    University of California, San Francisco, CA 94143, USA
    Biotechniques 37:534-6. 2004
  61. pmc Mutational effects depend on ploidy level: all else is not equal
    Aleeza C Gerstein
    Department of Zoology and Biodiversity Research Center, University of British Columbia, Vancouver, British Columbia, Canada
    Biol Lett 9:20120614. 2013
    ..This empirical result demonstrates that it cannot be assumed that mutations will have the same effect in haploids and homozygous diploids...
  62. pmc Relationship between switching and mating in Candida albicans
    David R Soll
    Department of Biological Sciences, The University of Iowa, Iowa City, Iowa 52242, USA
    Eukaryot Cell 2:390-7. 2003
  63. ncbi HDTIC-1 and HDTIC-2, two compounds extracted from Astragali Radix, delay replicative senescence of human diploid fibroblasts
    Peichang Wang
    Department of Biochemistry and Molecular Biology, Health Science Center, Peking University, Beijing 100083, PR China
    Mech Ageing Dev 124:1025-34. 2003
    ..The differences of optimum concentrations of HDTIC-1 (0.1 microM) and HDTIC-2 (1.0 microM) for delaying senescence also indicate that the structure of HDTIC may be very sensitive to its activity...
  64. pmc Tocotrienol-rich fraction prevents cell cycle arrest and elongates telomere length in senescent human diploid fibroblasts
    Suzana Makpol
    Department of Biochemistry, Faculty of Medicine, National University of Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia
    J Biomed Biotechnol 2011:506171. 2011
    ..These findings confirmed the ability of tocotrienol-rich fraction in preventing HDFs cellular ageing by restoring telomere length and telomerase activity, reducing damaged DNA, and reversing cell cycle arrest associated with senescence...
  65. pmc Arabidopsis hybrid speciation processes
    Roswitha Schmickl
    Centre for Organismal Studies Heidelberg, Department of Biodiversity and Plant Systematics, University of Heidelberg, D 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 108:14192-7. 2011
    ..lyrata. This cytotype was able to escape from the narrow ecological niche occupied by diploid A. lyrata ssp. petraea on limestone outcrops by migrating northward into siliceous areas, leaving behind a trail of genetic differentiation...
  66. pmc Transcriptome analysis of functional differentiation between haploid and diploid cells of Emiliania huxleyi, a globally significant photosynthetic calcifying cell
    Peter von Dassow
    Evolution du Plancton et PaleOceans, Station Biologique de Roscoff, CNRS UPMC UMR7144, 29682 Roscoff, France
    Genome Biol 10:R114. 2009
    ..huxleyi normalized cDNA libraries to identify genes involved in important processes specific to each life phase (2N calcification or 1N motility), and to better understand the haploid phase of this prominent haplo-diplontic organism...
  67. pmc The parasitoid wasp Nasonia: an emerging model system with haploid male genetics
    John H Werren
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Cold Spring Harb Protoc 2009:pdb.emo134. 2009
    ..Advances in the genetics of this system also open a path for improvement of parasitoid insects as agents of pest control...
  68. ncbi Generation of a large set of genetically tractable haploid and diploid Saccharomyces strains
    Francisco A Cubillos
    Queen s Medical Centre, Institute of Genetics, University of Nottingham, Nottingham, UK
    FEMS Yeast Res 9:1217-25. 2009
    ..Three versions of each strain (haploid Mat a and Mat alpha and diploid Mat a/alpha all as ura3::KanMX-Barcode) are available through the National Culture Yeast Collection...
  69. ncbi Inferring population structure and genetic diversity of broad range of wild diploid alfalfa (Medicago sativa L.) accessions using SSR markers
    Muhammet Sakiroğlu
    Department of Biology, Kafkas University, 36100, Kars, Turkey
    Theor Appl Genet 121:403-15. 2010
    ..This research provides a baseline from which to systematically evaluate variability in tetraploid alfalfa and serves as a starting point for exploring diploid alfalfa for genetic and breeding experiments...
  70. pmc Ploidy influences cellular responses to gross chromosomal rearrangements in Saccharomyces cerevisiae
    Paul P Jung
    Department of Genetics, Genomics and Microbiology, University of Strasbourg, CNRS, UMR, Strasbourg, France
    BMC Genomics 12:331. 2011
    ..To obtain new insights on the role of ploidy, the cellular effects of GCRs were compared between the haploid and diploid contexts...
  71. pmc SIRT1 overexpression antagonizes cellular senescence with activated ERK/S6k1 signaling in human diploid fibroblasts
    Jing Huang
    Peking University Research Center on Aging, Beijing, People s Republic of China
    PLoS ONE 3:e1710. 2008
    ..These findings suggested that SIRT1-promoted cell proliferation and antagonized cellular senescence in human diploid fibroblasts may be, in part, via the activation of ERK/ S6K1 signaling...
  72. pmc Sequence-level analysis of the diploidization process in the triplicated FLOWERING LOCUS C region of Brassica rapa
    Tae Jin Yang
    Brassica Genomics Team, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441 707, Korea
    Plant Cell 18:1339-47. 2006
    ..The gene content of the recently duplicated B. rapa genome segments is identical, but intergenic sequences differ...
  73. ncbi Rax1, a protein required for the establishment of the bipolar budding pattern in yeast
    Atsushi Fujita
    National Institute of Advanced Industrial Science and Technology, Tsukuba Central 6 4, 1 1 Higashi, Tsukuba 305 8566, Japan
    Gene 327:161-9. 2004
    ..Interestingly, Rax1 contains a C-terminal domain possessing some similarity to insulin-related peptides. Our results suggest that Rax1 is necessary for the establishment of the bipolar budding landmark...
  74. pmc Homoeolog-specific transcriptional bias in allopolyploid wheat
    Alina R Akhunova
    Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506, USA
    BMC Genomics 11:505. 2010
  75. ncbi A genetic linkage map of microsatellite, gene-specific and morphological markers in diploid Fragaria
    D J Sargent
    East Malling Research, New Road, East Malling, West Malling, Kent ME19 6BJ, UK
    Theor Appl Genet 109:1385-91. 2004
    ..The map also provides a sound basis for linkage map transfer to the cultivated octoploid strawberry...
  76. pmc Spontaneous loss of heterozygosity in diploid Saccharomyces cerevisiae cells
    M Hiraoka
    Department of Molecular Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630 0101, Japan
    Genetics 156:1531-48. 2000
  77. pmc Population genetic inference from resequencing data
    Rong Jiang
    Department of Oncology, University of Cambridge, Cambridge CB2 0RE, UK
    Genetics 181:187-97. 2009
    ..We also present two refinements of our methods: one that is more robust to sequencing errors and another that can be used when no reference genome is available...
  78. ncbi Fall and rise of satellite repeats in allopolyploids of Nicotiana over c. 5 million years
    Blazena Koukalova
    Institute of Biophysics, Academy of Sciences of the Czech Republic, CZ 612 65 Brno, Czech Republic
    New Phytol 186:148-60. 2010
    ..Instead we propose that this mechanism occurs with the deletion of large chromatin blocks and reamplification, perhaps via rolling circle replication...
  79. ncbi PPAR{gamma} accelerates cellular senescence by inducing p16INK4{alpha} expression in human diploid fibroblasts
    Qini Gan
    Research Center on Aging, Department of Biochemistry and Molecular Biology, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100083, People s Republic of China
    J Cell Sci 121:2235-45. 2008
    ..In addition, our data indicate that phosphorylation of PPARgamma decreased with increased cell passage. Our results provide a possible molecular mechanism underlying the regulation of cellular senescence...
  80. pmc New insights into the origin of the B genome of hexaploid wheat: evolutionary relationships at the SPA genomic region with the S genome of the diploid relative Aegilops speltoides
    Jerome Salse
    UMR INRA 1165 CNRS 8114 UEVE Unité de Recherche en Génomique Végétale, 2, rue Gaston Crémieux, CP5708, 91057 Evry Cedex, France
    BMC Genomics 9:555. 2008
    ..speltoides (2n = 14) to that of the A, B and D genomes co-resident in the hexaploid wheat species (Triticum aestivum, AABBDD, 2n = 42)...
  81. pmc Comparative mapping of Brassica juncea and Arabidopsis thaliana using Intron Polymorphism (IP) markers: homoeologous relationships, diversification and evolution of the A, B and C Brassica genomes
    Priya Panjabi
    Centre for Genetic Manipulation of Crop Plants, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India
    BMC Genomics 9:113. 2008
    ..thaliana and analyzed the arrangement of 24 (previously described) genomic block segments in the A, B and C Brassica genomes to study the evolutionary events contributing to karyotype variations in the three diploid Brassica genomes...
  82. pmc Intra-individual polymorphism in diploid and apomictic polyploid hawkweeds (Hieracium, Lactuceae, Asteraceae): disentangling phylogenetic signal, reticulation, and noise
    Judith Fehrer
    Institute of Botany, Academy of Sciences of the Czech Republic, Pruhonice, Czech Republic
    BMC Evol Biol 9:239. 2009
    ..Concentrating on the supposed major evolutionary units, we used nuclear ribosomal (ETS) and chloroplast (trnT-trnL) sequences in order to disentangle the phylogenetic relationships and to infer the origins of the polyploids...
  83. pmc Concerted evolution of rDNA in recently formed Tragopogon allotetraploids is typically associated with an inverse correlation between gene copy number and expression
    Roman Matyasek
    Institute of Biophysics, Academy of Sciences of the Czech Republic, v v i Laboratory of Molecular Epigenetics, Kralovopolska 135, CZ 612 65 Brno, Czech Republic
    Genetics 176:2509-19. 2007
    ..Paradoxically, despite their low copy number, repeats of T. dubius origin dominate rDNA transcription in most populations studied, i.e., rDNA units that are genetic losers (copy numbers) are epigenetic winners (high expression)...
  84. ncbi Conversion of diploidy to haploidy
    H Yan
    Howard Hughes Medical Institute, The Oncology Center, Department of Mathematical Sciences, The Johns Hopkins University, Baltimore, Maryland 21231, USA
    Nature 403:723-4. 2000
  85. pmc A role for the actin cytoskeleton of Saccharomyces cerevisiae in bipolar bud-site selection
    S Yang
    Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    J Cell Biol 136:111-23. 1997
  86. ncbi Sympatric diploid and hexaploid cytotypes of Senecio carniolicus (Asteraceae) in the Eastern Alps are separated along an altitudinal gradient
    Peter Schönswetter
    Department of Biogeography and Botanical Garden, University of Vienna, Rennweg 14, Vienna, 1030, Austria
    J Plant Res 120:721-5. 2007
    ..This suggests the presence of ecological differentiation between cytotypes with the diploid possessing the broader ecological niche. No tetraploids were detected, indicating the presence of strong crossing barriers...
  87. pmc Genome changes after gene duplication: haploidy vs. diploidy
    Cheng Xue
    GuangDong Institute for Monitoring Laboratory Animals, Guangzhou, 510260, China
    Genetics 186:287-94. 2010
    ..Since genome size and the number of duplicate genes observed in genomes increase from haploid to diploid organisms, diploidy might provide more evolutionary probabilities through gene duplication...
  88. ncbi Order out of chaos in the hybrid plant nucleus
    R N Jones
    Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth SY23 3DA, UK
    Cytogenet Genome Res 126:376-89. 2009
    ..The nature of the resolution of nuclear conflicts in diploid hybrids and in allopolyploids is discussed...
  89. ncbi Spontaneous mutagenesis in haploid and diploid Saccharomyces cerevisiae
    Gaku Ohnishi
    Graduate School of Life Sciences, Tohoku University, Sendai 980 8577, Japan
    Biochem Biophys Res Commun 325:928-33. 2004
  90. ncbi Haploids adapt faster than diploids across a range of environments
    A C Gerstein
    Biodiversity Research Centre and Department of Zoology, The University of British Columbia, Vancouver, BC, Canada
    J Evol Biol 24:531-40. 2011
    ..These results are consistent with theory that predicts haploids should evolve faster than diploids at large population sizes...
  91. ncbi Single-gene deletions that restore mating competence to diploid yeast
    Tom Schmidlin
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    FEMS Yeast Res 8:276-86. 2008
    ..In summary, this study underscores and elaborates upon predicted pathways by which mutations restore mating function to yeast diploids and identifies new mutants warranting further study...
  92. ncbi Coevolution of A and B genomes in allotetraploid Triticum dicoccoides
    A Belyayev
    Institute of Evolution, Haifa University, Israel
    Genome 43:1021-6. 2000
    ..We propose that the detected high level of intergenomic invasion in old polyploids might reflect general tendencies in speciation and stabilization of the allopolyploid genome...
  93. ncbi Absence of single-locus complementary sex determination in the braconid wasps Asobara tabida and Alysia manducator
    L W Beukeboom
    Institute of Evolutionary and Ecological Sciences, University of Leiden, PO Box 9516, NL 2300 RA Leiden, The Netherlands
    Heredity (Edinb) 84:29-36. 2000
    ..Therefore, we conclude that sl-CSD is absent in these species. This study is the first to show the lack of sl-CSD in species of the Braconidae family and that hymenopteran sex-determining mechanisms can vary, even within a family...
  94. ncbi Tracking ancient polyploids: a retroposon insertion reveals an extinct diploid ancestor in the polyploid origin of belladonna
    Yao Wu Yuan
    State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, People s Republic of China
    Mol Biol Evol 23:2263-7. 2006
    ..This case may suggest that retroposons are promising molecular markers to study polyploid evolution...
  95. ncbi Direct mating between diploid sake strains of Saccharomyces cerevisiae
    Shinji Hashimoto
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube, 755 8611, Japan
    Appl Microbiol Biotechnol 69:689-96. 2006
    ..Isolation of mating-competent auxotrophic mutants directly from industrial yeast strains allows crossbreeding to construct polyploids suitable for industrial use without dependence on sporulation...
  96. ncbi Analysis of genetic alterations associated with DNA diploidy, aneuploidy and multiploidy in gastric cancers
    Tamotsu Sugai
    Division of Pathology, Central Clinical Laboratory, Iwate Medical University, Morioka, Japan
    Oncology 68:548-57. 2005
    Recent studies have shown a close association between DNA ploidy status (diploidy, aneuploidy and multiploidy) identified by the crypt isolation technique and specific genetic alterations in colorectal carcinomas...
  97. pmc Apparent ploidy effects on silencing are post-transcriptional at HML and telomeres in Saccharomyces cerevisiae
    Jenny M McLaughlan
    Centre for Genetics and Genomics, Queen s Medical Centre, University of Nottingham, Nottingham, United Kingdom
    PLoS ONE 7:e39044. 2012
    ..The post-transcriptional effects seen in this study, illustrate the often ignored need to measure RNA levels when assaying transcriptional silencing in Saccharomyces cerevisiae...
  98. ncbi Interstitial chromatin alteration causes persistent p53 activation involved in the radiation-induced senescence-like growth arrest
    Masatoshi Suzuki
    Division of Radiation Biology, Department of Radiology and Radiation Biology, Nagasaki University, 1 14 Bunkyo machi, Nagasaki 852 8521, Japan
    Biochem Biophys Res Commun 340:145-50. 2006
    ..Our results indicate that chromatin alteration in interstitial chromosomal regions is the most likely cause of continuous activation of p53, which results in the induction of SLGA by ionizing radiation...
  99. pmc Differential regulation of gene products in newly synthesized Brassica napus allotetraploids is not related to protein function nor subcellular localization
    Warren Albertin
    UMR de Genetique Vegetale, INRA CNRS UPSud INA P G, La Ferme du Moulon, 91190 Gif sur Yvette, France
    BMC Genomics 8:56. 2007
    ..Here, we report the in silico functional characterization of the "non-additive" proteins (the ones with a non-additive pattern of regulation) in synthetic B. napus...
  100. ncbi FLO11 mediated filamentous growth of the yeast Saccharomyces cerevisiae depends on the expression of the ribosomal RPS26 genes
    Axel W Strittmatter
    Institute of Microbiology and Genetics, Georg August Universitat, 37077, Gottingen, Germany
    Mol Genet Genomics 276:113-25. 2006
    ..This suggests that the amount of Rps26p is critical for accurate translation of the FLO11 mRNA, and therefore for the dimorphic switch of the bakera9s yeast from a single cell yeast to an adhesive filamentous growth form...
  101. ncbi Analysis of two abundant, highly related satellites in the allotetraploid Nicotiana arentsii using double-strand conformation polymorphism analysis and sequencing
    Roman Matyasek
    Institute of Biophysics, Academy of Sciences of the Czech Republic, vvi, Brno, Czech Republic
    New Phytol 192:747-59. 2011
    ..arentsii allotetraploid. We propose that the sequence and structural divergence of subtelomeric satellites may render allopolyploid chromosomes less vulnerable to intergenomic exchanges...

Research Grants68

  1. Genetics of Cryptococcus sexual reproduction
    Joseph Heitman; Fiscal Year: 2013
    ..on the de novo generation of genotypic and phenotypic plasticity by sexual reproduction involving aneuploidy and diploidy. Aim 3 addresses sexual reproduction and the basis of hypervirulence in the C. gattii Pacific Northwest outbreak...
  2. Genomic profiling of yeast resistance to AFB1, a P450-activated carcinogen
    MICHAEL THOMAS FASULLO; Fiscal Year: 2013
    ..The project will be a valuable training tool in systems and computational biology. ..
    Nancy M Hollingsworth; Fiscal Year: 2013
    ..The knowledge gained from these studies is likely to be relevant to mammals, given the evolutionary conservation of meiosis between yeast and mammals. ..
  4. The Synapsis Checkpoint in C. elegans Meiosis
    Needhi Bhalla; Fiscal Year: 2013
  5. Alteration of ER:mitochondrial contacts by human cytomegalovirus infection
    ..This exploratory study should set the stage for more detailed analyses of the mode of action of HCMV UL37 proteins and the possibility that they may serve as targets for intervention. ..
  6. Development of a human Oct-4 gene-targeted reporter cell line
    GREGORY LENO; Fiscal Year: 2010
    ..Efficiently reprogrammed human cells have viable commercial applications in regenerative medicine. ..
  7. Reverse genetics to develop a second generation Rift Valley fever vaccine
    Tetsuro Ikegami; Fiscal Year: 2013
    ..We will identify and characterize the attenuation mutations of existing MP-12 vaccine and generate further attenuated but immunogenic second generation MP-12 vaccines. ..
  8. Mechanisms of Nuclear and Cell Fusion in Yeast
    MARK DAVID ROSE; Fiscal Year: 2013
    ..We will use advanced imaging and genetic methods to identify Kar5p's role(s) in nuclear membrane fusion. ..
  9. Arsenic Induced Miotic Arrest Associated Apoptosis
    J Christopher States; Fiscal Year: 2009
  10. Characterization of the Synapis Checkpoint in C. elegans Meiosis
    Needhi Bhalla; Fiscal Year: 2010
    ..Occasionally, an embryo inherits an extra chromosome that is not lethal but can cause cancer predisposition or serious developmental defects. ..
  11. Impact of hepatocyte lineage life history dynamics on liver homeostasis in the ag
    Edward E Schmidt; Fiscal Year: 2013
    ..abstract_text> ..
  12. Role of CMV kinase in regulating infection of ESC-derived neuroprogenitor cells
    Tarin M Bigley; Fiscal Year: 2013
    ..My studies will determine if a pUL97 kinase inhibitor is suitable for treating CMV infected neonates and possibly CMV infected mothers at risk of congenital infection. ..
  13. Mechanisms of megakaryocyte maturation
    Diane S Krause; Fiscal Year: 2013
    ..In addition, the data obtained will reveal the molecular regulation and functions of MKL1, a transcriptional cofactor in the RhoA/SRF pathway, which is dysregulated in Myelodysplasia (MDS) especially that associated with 5q- syndrome. ..
  14. Role of CDC14B in mouse oocyte maturation
    KAREN A SCHINDLER; Fiscal Year: 2010
    ..The major goal of this proposal is to determine the role for the CDC14B phosphatase during meiosis in females using the mouse model ..
  15. Statistical Models in Epigenomics
    Kimberly D Siegmund; Fiscal Year: 2011
    ..We propose to develop mathematical models that can retrospectively reconstruct tumor histories, allowing us to address important biological questions about the growth and spread of cancer. ..
  16. Use of Genetically Engineered T cells Targeting Tumor Stroma to Treat Lung Cancer
    STEVEN MARK ALBELDA; Fiscal Year: 2013
    ..If our strategy proves effective, this project will likely have additional significace beyond lung cancer, since FAP could be targeted in many tumors. ..
  17. Meiotic Chromosome Segregation in C. elegans
    Anne M Villeneuve; Fiscal Year: 2013
    ..abstract_text> ..
  18. Dissection of the Inner Centromere Regulatory Network
    P Todd Stukenberg; Fiscal Year: 2013
    ..This combination of biochemical, cell biological and in vivo techniques provides unique experimental power to dissect this important problem. ..
  19. Chromosome pairing and condensins
    Giovanni Bosco; Fiscal Year: 2012
    ..The experiments proposed in this grant application will have a direct and significant impact on the advancement of biomedical research and human health. ..
    Martin Flajnik; Fiscal Year: 2009
    ..tropicalis), we will begin to analyze the modes of silencing in the tetraploids and eventually the higher order octo- and dodecaploid individuals. ..
  21. JC Virus and Human Colorectal Neoplasia
    CLEMENT R BOLAND; Fiscal Year: 2013
    ..It is the long-term aim of this project to determine whether we should develop a vaccine that will prevent primary infection by JC virus, and prevent the occurrence of colorectal cancer. ..
  22. Defensin gene copy number and mucosal innate immunity
    JAY V contact SOLNICK; Fiscal Year: 2010
    ..We hypothesize that differences in BD2 GCN affect the host inflammatory response, and thus the propensity to develop disease after infection with H. pylori. ..
  23. Genetic Studies in Gestational Trophoblastic Disease
    Ignatia Van den Veyver; Fiscal Year: 2009
    ..Together these studies will benefit our understanding of the pathogenesis of CHM and the role of imprinted genes in placental function and fetal development. ..
  24. Heritable variation of mRNA decay rates: An unappreciated source of gene express
    JOSHUA MICHAEL AKEY; Fiscal Year: 2013
    ..Many human diseases, such as cancer, arise from improper gene expression. This project will provide critical new insights into how genetic variation influences gene expression levels and ultimately the molecular mechanisms of disease. ..
  25. Polyploidy, aneuploidy and genome stability
    DAVID S PELLMAN; Fiscal Year: 2013
  26. Single Centriole-Mediated Gynogenesis in the Zebrafish
    Francisco J Pelegri; Fiscal Year: 2012
    ..In addition, our studies will identify genes involved in spermatogenesis and early embryonic development. ..
  27. Trisomy 8 in hematopoiesis and myeloid leukemia
    David J Gordon; Fiscal Year: 2013
    ..I have assembled an excellent mentoring and advisory committee, consisting of Dr. David Pellman, Dr. Scott Armstrong, Dr. George Daley and Dr. Nathanael Gray, that will guide my research and training experiences. ..
  28. A new system for mammalian cell genetics using a haploid genomic context
    Thijn Brummelkamp; Fiscal Year: 2010
    ..Following its development and validation, we foresee this novel strategy for mammalian genetic screens would be pertinently useful in more broad applications of biomedical research. ..
  29. Recombination pathway and partner choice during C. elegans meiosis
    DIANA ELIZABETH LIBUDA; Fiscal Year: 2013
    ..Overall, these studies will reveal how recombination pathway and partner preferences ensure that chromosomes form the connections necessary for chromosome segregation and repair DSBs for maintaining genomic integrity. ..
  30. Single Molecule Studies of Recombination and Chromosome Pairing in Meiosis
    Richard Fishel; Fiscal Year: 2013
    ..Our unique single molecule approach should enhance the quantitative understanding of mechanical processes that lead to viable gamete formation as well as the fine line between dysfunctions that lead to infertility and genetic disease. ..
  31. Genome-methylome Interactions
    Kun Zhang; Fiscal Year: 2013
    MICHAEL FINLEY SUMMERS; Fiscal Year: 2013
  33. Identification of a cryptic meiosis in the fungal pathogen C. albicans
    RACQUEL K SHERWOOD; Fiscal Year: 2010
    ..albicans and the establishment of a meiotic pathway will open up the study of this organism to classical yeast genetic approaches. ..
  34. Role of CDC14B in mouse oocyte maturation
    KAREN A SCHINDLER; Fiscal Year: 2013
    ..Thus, the PI will be well-positioned to compete for additional funding and to have a successful independent research career. ..
    WOODRING ERIK WRIGHT; Fiscal Year: 2012
    ..Knowledge of the structure and processing of telomeres and the mechanisms by which they are maintained will provide targets for the development of new drugs to treat cancer and age-related diseases. ..
  36. Genetic Determinants of Genomic Plasticity and Chromosome Evolution
    Forrest A Spencer; Fiscal Year: 2010
    ..In humans, distinct chromosome nondisjunction signatures that reveal specific dysfunctional pathways could enhance diagnosis and treatment of disease. ..
  37. KCNK9 Imprinting in Breast Cancer Progression and Metastasis
    Victoria L Seewaldt; Fiscal Year: 2013
    ..PHS 398/2590 (Rev. 09/04, Reissued 4/2006) Page Continuation Format Page ..
  38. Selection For Drug Resistance To Study Genetic Instability
    DARIO CABRERA; Fiscal Year: 2010
    ..This project will use brewer's yeast to study how cells evolve to become genetically unstable, with the aim of improving cancer diagnosis and therapy. ..
  39. Novel Biomarkers in Rheumatoid Arthritis
    ..In Aim 2, we propose that C4B deficiency shapes RA phenotype and B cell hyperactivity (serum CXCL13, IgG and autoantibody levels). ..
  40. In vivo Detection and Imaging of Epigenetic Histone Modifications and Modifying E
    Warren R Zipfel; Fiscal Year: 2013
  41. How eukaryotic pathogens explore the fitness landscape by mitotic recombination
    Tim James; Fiscal Year: 2013
  42. Genetic regulation of genome stability in yeast
    THOMAS PETES; Fiscal Year: 2013
    ..Finally, we plan to map LOH events in mammalian cells that are exposed to topoisomerase inhibitors. ..
  43. Sequencing yeast lines to measure rates of neutral and deleterious mutations
    Mark L Siegal; Fiscal Year: 2013
    ..Haploids have been used previously to estimate mutational spectra in yeast, but diploidy has several critical advantages, including better shielding of deleterious mutations, avoidance of genomic ..
  44. Modulation of DNA double-strand break repair by posttranslational modification.
    Wolf Dietrich Heyer; Fiscal Year: 2013
    ..We will combine biochemical and genetic approaches to determine the mechanism of positive feedback regulation on Mec1 kinase. ..
  45. Mechanisms of Gonococcal Pilin Antigenic and Phase Variation
    Hank S Seifert; Fiscal Year: 2013
  46. KillerRed Assisted Mutagenesis to discover cancer drug resistance genes
    Daniel G Jay; Fiscal Year: 2013
    ..As a generalized low cost approach for gain-of-function/loss-of-function selection in somatic cells, KRAM will have wide application across biomedicine and be a transformative technology. ..
    Timothy A Graubert; Fiscal Year: 2010
    ..We will use state of the art sequencing technology to define the genetic basis of this disease. This represents a "Grand Opportunity" to rapidly increase knowledge that will have long-term benefits for the care of these patients. ..
  48. High Definition Clonal Analyses of Archival Pancreatic Adenocarcinoma Samples
    Michael T Barrett; Fiscal Year: 2012
    ..These will include diploid and aneuploid cell populations from formulin fixed paraffin embedded (FFPE) samples of pancreatic adenocarcinomas. ..
  49. A general Bayesian polymorphism discovery tool
    Gabor Marth; Fiscal Year: 2009
  50. Characterization of a Meiotic Crossover Surveillance System
    Judith L Yanowitz; Fiscal Year: 2013
    ..These complementary approaches will provide significant insights into a surveillance system that monitors crossover formation on each chromosome and allows for timely progression through meiosis. ..
  51. Cell growth control by cell and organelle size-dependent ribosome biogenesis
    Clifford P Brangwynne; Fiscal Year: 2012
  52. Mechanistic basis for endosomal dysfunction in frontotemporal dementia linked to
    CHARLES G ODORIZZI; Fiscal Year: 2012
  53. Transposons, RNA Interference and Heterochromatin
    Robert A Martienssen; Fiscal Year: 2013
    ..We have preliminary evidence that systemically mobile small RNA accumulate in the gametes, from which they are passed into the fertilized zygote, providing a mechanism for transgenerational inheritance. ..
  54. Optical Sensing of Dysplasia and Aneuploidy in Upper GI Endoscopy
    Satish K Singh; Fiscal Year: 2013
    ..In phase two of the study, the algorithm trained with the data collected in the previous phase will be tested for real-time detection of dysplasia to increase the per-biopsy yield of dysplastic and/or aneuploidy tissue. ..
  55. FSHD: Chromatin Structure, Looping, & Expression
    Melanie Ehrlich; Fiscal Year: 2009
    ..The proposed research should elucidate new aspects of long-distance control of gene expression as well as lending clinically useful insights into this currently intractable disease. ..
    Miriam Zolan; Fiscal Year: 2009
    ..abstract_text> ..
  57. The Yeast Centrosome: Phosphoproteome and Phospho-dependent Regulation
    Jamie Keck; Fiscal Year: 2009
    ..The proposed project will identify these phosphorylation sites within the centrosome and study how altered phosphorylation states can cause chromosome segregation defects in the cell. ..
  58. Evolution of Meiotic Mechanisms in Divergent Cytological Environments
    Kevin Wright; Fiscal Year: 2013
    ..In a complimentary experiment, I will conduct an artificial selection experiment on gamete viability in the synthetic autotetraploid and genetically map loci that ensure proper meiosis in the tetraploid. ..
    John R Pringle; Fiscal Year: 2010
    ..and associate with the plasma membrane? What other proteins do they organize at the cell cortex? What are the roles of the septins both in ordinary cytokinesis and in the specialized process by which spores are formed? ..
  60. Quantitative Trait Dissection by Reciprocal Hemizygosity
    RONALD WAYNE DAVIS; Fiscal Year: 2013
    ..The establishment of tools for identifying quantitative trait genes and results on their functional reconstitution and genetic engineering will provide the best data for approaching complex traits for medical studies. ..
  61. The Origins of Chromosomal Instability in Human Tumor Cells
    Nicholas J Dyson; Fiscal Year: 2013
    ..We will determine whether similar changes occur when pRB is inactivated by deregulated cdk activity and when pRB-related proteins are targeted. ..
  62. Single-Cell Sequencing of Breast Tumors to Investigate Genome Evolution
    Nicholas Navin; Fiscal Year: 2013
  63. Comparative and functional proteomics of germline chromatin in C. elegans
    KENNETH HILLERS; Fiscal Year: 2009
    ..Fusion of two haploid gametes at fertilization recreates diploidy in the next generation...
    Greg Hunt; Fiscal Year: 2000
    ..3 cM with 95% confidence. It is proposed to use STS primers designed from the RAPD markers, SSCP polymorphisms and further development of another set of RAPD markers from 1000 10-mer primers, screened in bulk. ..
    Dean Dawson; Fiscal Year: 1992
    ..The goal here is to acquire a set of mutant strains and cloned genes which can be used as tools to examine how this system functions in yeast and whether it is common to other organisms...
  66. Control of Genomic Stability by Emi1 and Securin
    NORMAN LEHMAN; Fiscal Year: 2007
    ..abstract_text> ..
  67. 2004 & 2006 Meiosis Gordon Conference
    DOUGLAS BISHOP; Fiscal Year: 2005
    ..The Meiosis Gordon Conference thus provides the most important forum for presenting and discussing new findings regarding a process that is central to sexual reproduction and to maintenance of genome integrity. ..
    ANNE DRANGINIS; Fiscal Year: 1999
    ..A cell surface molecule that is critically required for filamentation and invasion such as FLO11 may be a promising target for anti-fungal therapies. ..