gene deletion

Summary

Summary: A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.

Top Publications

  1. ncbi Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
    M S Longtine
    Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
    Yeast 14:953-61. 1998
  2. ncbi Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe
    J Bahler
    Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
    Yeast 14:943-51. 1998
  3. ncbi Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae
    A L Goldstein
    Department of Microbiology, 3020 Duke University Medical Center, Durham, NC 27710, USA
    Yeast 15:1541-53. 1999
  4. ncbi Functional profiling of the Saccharomyces cerevisiae genome
    Guri Giaever
    Stanford Genome Technology Center, Stanford University, Palo Alto, California 94304, USA
    Nature 418:387-91. 2002
  5. pmc A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors
    Hildur V Colot
    Department of Genetics, Dartmouth Medical School, HB7400, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 103:10352-7. 2006
  6. pmc Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe
    Dong Uk Kim
    Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology, Yuseong, Daejeon, Korea
    Nat Biotechnol 28:617-23. 2010
  7. ncbi The small RNA chaperone Hfq and multiple small RNAs control quorum sensing in Vibrio harveyi and Vibrio cholerae
    Derrick H Lenz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Cell 118:69-82. 2004
  8. ncbi Conditional gene targeting in macrophages and granulocytes using LysMcre mice
    B E Clausen
    Institute for Genetics, University of Cologne, Germany
    Transgenic Res 8:265-77. 1999
  9. pmc Extension of chronological life span in yeast by decreased TOR pathway signaling
    R Wilson Powers
    Department of Genome Sciences and Medicine, The Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195, USA
    Genes Dev 20:174-84. 2006
  10. ncbi Global gene deletion analysis exploring yeast filamentous growth
    Owen Ryan
    Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5S 3E1, Canada
    Science 337:1353-6. 2012

Detail Information

Publications331 found, 100 shown here

  1. ncbi Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
    M S Longtine
    Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
    Yeast 14:953-61. 1998
    ..the heterologous Schizosaccharomyces pombe his5+ or Escherichia coli kan(r) gene, these plasmids allow gene deletion, gene overexpression (using the regulatable GAL1 promoter), C- or N-terminal protein tagging [with GFP(S65T), ..
  2. ncbi Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe
    J Bahler
    Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
    Yeast 14:943-51. 1998
    ..In most cases, the efficiency of homologous integration was > or = 50%, and the lowest efficiency encountered was 17%. The methodology and constructs described here should greatly facilitate analysis of gene function in S. pombe...
  3. ncbi Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae
    A L Goldstein
    Department of Microbiology, 3020 Duke University Medical Center, Durham, NC 27710, USA
    Yeast 15:1541-53. 1999
    ..These attributes make the cassettes ideally suited for creating S. cerevisiae strains with multiple mutations within a single strain...
  4. ncbi Functional profiling of the Saccharomyces cerevisiae genome
    Guri Giaever
    Stanford Genome Technology Center, Stanford University, Palo Alto, California 94304, USA
    Nature 418:387-91. 2002
    Determining the effect of gene deletion is a fundamental approach to understanding gene function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype are often missed...
  5. pmc A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors
    Hildur V Colot
    Department of Genetics, Dartmouth Medical School, HB7400, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 103:10352-7. 2006
    ..Overall, the methodology, which achieves high-throughput gene disruption at an efficiency >90% in this filamentous fungus, promises to be applicable to other eukaryotic organisms that have a low frequency of homologous recombination...
  6. pmc Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe
    Dong Uk Kim
    Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology, Yuseong, Daejeon, Korea
    Nat Biotechnol 28:617-23. 2010
    ....
  7. ncbi The small RNA chaperone Hfq and multiple small RNAs control quorum sensing in Vibrio harveyi and Vibrio cholerae
    Derrick H Lenz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Cell 118:69-82. 2004
    ..We propose that Hfq, together with these sRNAs, creates an ultrasensitive regulatory switch that controls the critical transition into the high cell density, quorum-sensing mode...
  8. ncbi Conditional gene targeting in macrophages and granulocytes using LysMcre mice
    B E Clausen
    Institute for Genetics, University of Cologne, Germany
    Transgenic Res 8:265-77. 1999
    ..In contrast, no significant deletion was observed in tail DNA or purified T and B cells. Taken together, LysMcre mice allow for both specific and highly efficient Cre-mediated deletion of loxP-flanked target genes in myeloid cells...
  9. pmc Extension of chronological life span in yeast by decreased TOR pathway signaling
    R Wilson Powers
    Department of Genome Sciences and Medicine, The Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195, USA
    Genes Dev 20:174-84. 2006
    ..We developed a high-throughput assay to determine CLS for approximately 4800 single-gene deletion strains of yeast, and identified long-lived strains carrying mutations in the conserved TOR pathway...
  10. ncbi Global gene deletion analysis exploring yeast filamentous growth
    Owen Ryan
    Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5S 3E1, Canada
    Science 337:1353-6. 2012
    ..cerevisiae and C. albicans...
  11. pmc A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast
    U Gueldener
    Heinrich Heine Universitat, Institut fur Mikrobiologie, Universitatsstrasse 1, Geb 26 12 01 64, 40225 Dusseldorf, Germany
    Nucleic Acids Res 30:e23. 2002
    ..The set of disruption cassettes and Cre expression plasmids described here represents a significant further development of the marker rescue system, which is ideally suited to functional analysis of the yeast genome...
  12. pmc Relative impact of nucleotide and copy number variation on gene expression phenotypes
    Barbara E Stranger
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
    Science 315:848-53. 2007
    ..Interrogation of the genome for both types of variants may be an effective way to elucidate the causes of complex phenotypes and disease in humans...
  13. ncbi Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi
    Jae Hyuk Yu
    Department of Food Microbiology and Toxicology, The University of Wisconsin, Madison, WI 53706, USA
    Fungal Genet Biol 41:973-81. 2004
    ..To stream line the analysis of the transformants a relatively simple procedure for genomic DNA or total RNA isolation achieving approximately 100 samples/person/day is also presented...
  14. ncbi Systematic genetic analysis with ordered arrays of yeast deletion mutants
    A H Tong
    Banting and Best Department of Medical Research, University of Toronto, Toronto ON, Canada M5G 1L6
    Science 294:2364-8. 2001
    ..Systematic application of this approach should produce a global map of gene function...
  15. pmc Phenotype microarrays for high-throughput phenotypic testing and assay of gene function
    B R Bochner
    Biolog, Inc, Hayward, California 94545, USA
    Genome Res 11:1246-55. 2001
    ..Here, we provide data on phenotypic analysis of six strains and show that we can detect expected phenotypes as well as, in some cases, unexpected phenotypes...
  16. pmc A molecular mechanism of chronological aging in yeast
    Christopher R Burtner
    Department of Biochemistry, University of Washington, 1959 NE Pacific Street, Seattle, WA 98195, USA
    Cell Cycle 8:1256-70. 2009
    ..We conclude that acetic acid induced mortality is the primary mechanism of chronological aging in yeast under standard conditions...
  17. ncbi Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic beta cell-specific gene knock-outs using Cre recombinase
    C Postic
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 274:305-15. 1999
    ..Interestingly, hepatic GK knock-out mice also have impaired insulin secretion in response to glucose. These studies indicate that deficiencies in both beta cell and hepatic GK contribute to the hyperglycemia of MODY-2...
  18. ncbi Systematic identification of pathways that couple cell growth and division in yeast
    Paul Jorgensen
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8
    Science 297:395-400. 2002
    ..We determined cell size distributions for the complete set of approximately 6000 Saccharomyces cerevisiae gene deletion strains and identified approximately 500 abnormally small (whi) or large (lge) mutants...
  19. ncbi VE-Cadherin-Cre-recombinase transgenic mouse: a tool for lineage analysis and gene deletion in endothelial cells
    Jackelyn A Alva
    Department of Molecular, Cell and Developmental Biology and Molecular Biology Institute, UCLA, Los Angeles, California, USA
    Dev Dyn 235:759-67. 2006
    The ability to target gene deletion to a specific cellular compartment via the Cre/loxP system has been a powerful tool in the analysis of broadly expressed genes...
  20. pmc Strains and strategies for large-scale gene deletion studies of the diploid human fungal pathogen Candida albicans
    Suzanne M Noble
    Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94143 2200, USA
    Eukaryot Cell 4:298-309. 2005
    ..C. albicans is diploid, and current strategies for gene deletion typically involve repeated use of the URA3 selectable marker...
  21. pmc A comprehensive strategy enabling high-resolution functional analysis of the yeast genome
    David K Breslow
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 1700 4th Street, San Francisco, California 94158, USA
    Nat Methods 5:711-8. 2008
    ..Finally, by measuring the fitness of strains in the yeast deletion library, we addressed an enigma regarding the apparent prevalence of gene dispensability and found that most genes do contribute to growth...
  22. ncbi Emergent properties of reduced-genome Escherichia coli
    György Pósfai
    Institute of Biochemistry, Biological Research Center, H 6726 Szeged, Hungary
    Science 312:1044-6. 2006
    ..Eradication of stress-induced transposition evidently stabilized the MDS genomes and provided some of the new properties...
  23. ncbi Specialized transduction: an efficient method for generating marked and unmarked targeted gene disruptions in Mycobacterium tuberculosis, M. bovis BCG and M. smegmatis
    Stoyan Bardarov
    Dept of Microbiology and Immunology and Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Microbiology 148:3007-17. 2002
    ..tuberculosis, BCG or M. smegmatis which differ by defined mutations...
  24. ncbi A suppressor of two essential checkpoint genes identifies a novel protein that negatively affects dNTP pools
    X Zhao
    Department of Genetics and Development, Columbia University, College of Physicians and Surgeons, New York, New York 10032 2704, USA
    Mol Cell 2:329-40. 1998
    ..Finally, both in vivo and in vitro experiments show that Sml1 binds to Rnr1. We propose that Sml1 inhibits dNTP synthesis posttranslationally by binding directly to Rnr1 and that Mec1 and Rad53 are required to relieve this inhibition...
  25. ncbi The mouse Spo11 gene is required for meiotic chromosome synapsis
    P J Romanienko
    Genetics and Biochemistry Branch National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health 20892, Bethesda, MD, USA
    Mol Cell 6:975-87. 2000
    ..We speculate that there is an additional role for Spo11, after it generates DSBs, in synapsis...
  26. pmc Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum
    Martin C Jonikas
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94143, USA
    Science 323:1693-7. 2009
    ..The use of a quantitative reporter in a comprehensive screen followed by systematic analysis of genetic dependencies should be broadly applicable to functional dissection of complex cellular processes from yeast to human...
  27. ncbi Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme
    M Muramatsu
    Department of Medical Chemistry, Graduate School of Medicine, Institute for Virus Research, Kyoto University, Japan
    Cell 102:553-63. 2000
    ..These results suggest that AID may be involved in regulation or catalysis of the DNA modification step of both class switching and somatic hypermutation...
  28. pmc The chemical genomic portrait of yeast: uncovering a phenotype for all genes
    Maureen E Hillenmeyer
    Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, USA
    Science 320:362-5. 2008
    ..We found that 97% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition...
  29. ncbi BCR-ABL1 lymphoblastic leukaemia is characterized by the deletion of Ikaros
    Charles G Mullighan
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Nature 453:110-4. 2008
    ..These findings suggest that genetic lesions resulting in the loss of Ikaros function are an important event in the development of BCR-ABL1 ALL...
  30. pmc Strong association of de novo copy number mutations with autism
    Jonathan Sebat
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Science 316:445-9. 2007
    ..Affected genomic regions were highly heterogeneous and included mutations of single genes. These findings establish de novo germline mutation as a more significant risk factor for ASD than previously recognized...
  31. pmc Function and evolution of a minimal plastid genome from a nonphotosynthetic parasitic plant
    K H Wolfe
    Department of Biology, Indiana University, Bloomington 47405
    Proc Natl Acad Sci U S A 89:10648-52. 1992
    ..Remarkably, this massive pruning occurred with a virtual absence of gene order change...
  32. ncbi Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli
    B Karsten Tischer
    Cornell University, Ithaca, NY, USA
    Biotechniques 40:191-7. 2006
    ....
  33. ncbi Prostate-specific deletion of the murine Pten tumor suppressor gene leads to metastatic prostate cancer
    Shunyou Wang
    Howard Hughes Medical Institute, University of California Los Angeles School of Medicine, 90095, Los Angeles, CA, USA
    Cancer Cell 4:209-21. 2003
    ..This murine prostate cancer model provides a unique tool for both exploring the molecular mechanism underlying prostate cancer and for development of new targeted therapies...
  34. ncbi Microtubule nucleation at non-spindle pole body microtubule-organizing centers requires fission yeast centrosomin-related protein mod20p
    Kenneth E Sawin
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, EH9 3JR, United Kingdom
    Curr Biol 14:763-75. 2004
    ..These are the equatorial MTOC, which nucleates microtubules from the cell division site at the end of mitosis, and interphase MTOCs, which nucleate microtubules from multiple sites near the cell nucleus during interphase...
  35. ncbi TMPRSS2:ERG fusion-associated deletions provide insight into the heterogeneity of prostate cancer
    Sven Perner
    Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, EBRC 442A, 221 Longwood Avenue, Boston, MA 02115 6110, USA
    Cancer Res 66:8337-41. 2006
    ..The deletion as cause of TMPRSS2:ERG fusion is associated with clinical features for prostate cancer progression compared with tumors that lack the TMPRSS2:ERG rearrangement...
  36. pmc Genome-wide analysis of Fis binding in Escherichia coli indicates a causative role for A-/AT-tracts
    Byung Kwan Cho
    Department of Bioengineering, University of California San Diego, La Jolla, California 92093 0412, USA
    Genome Res 18:900-10. 2008
    ..These novel results provide new insight into how Fis modulates DNA topology at a genome scale and thus advance our understanding of the architectural bases of the E. coli nucleoid...
  37. pmc Genetic involvement of a cAMP-dependent protein kinase in a G protein signaling pathway regulating morphological and chemical transitions in Aspergillus nidulans
    K Shimizu
    Department of Plant Pathology and Microbiology, Texas A and M University, College Station, TX 77843 2132, USA
    Genetics 157:591-600. 2001
    ..These data provide genetic evidence that the FlbA/FadA signaling pathway regulating ST production and morphological development is partially mediated through PkaA...
  38. ncbi Synthetic genetic array analysis in Saccharomyces cerevisiae
    Amy Hin Yan Tong
    Methods Mol Biol 313:171-92. 2006
    ..In a typical SGA screen, a query mutation is crossed to an ordered array of approx 5000 viable gene deletion mutants (representing approximately 80% of all yeast genes) such that meiotic progeny harboring both mutations ..
  39. pmc Toward a comprehensive temperature-sensitive mutant repository of the essential genes of Saccharomyces cerevisiae
    Shay Ben-Aroya
    Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada
    Mol Cell 30:248-58. 2008
    The Saccharomyces cerevisiae gene deletion project revealed that approximately 20% of yeast genes are required for viability...
  40. ncbi Negative regulation of neural stem/progenitor cell proliferation by the Pten tumor suppressor gene in vivo
    M Groszer
    Howard Hughes Medical Institute, Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, CA 90095, USA
    Science 294:2186-9. 2001
    ..However, cell fate commitments of the progenitors were largely undisturbed. Our results suggest that PTEN negatively regulates neural stem cell proliferation...
  41. ncbi Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi
    Thomas A Volpe
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
    Science 297:1833-7. 2002
    ..We propose that double-stranded RNA arising from centromeric repeats targets formation and maintenance of heterochromatin through RNAi...
  42. pmc SUMO-targeted ubiquitin ligases in genome stability
    John Prudden
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 26:4089-101. 2007
    ..The DNA repair factor Rad60 and its human homolog NIP45, which contain SLDs, are candidate STUbL targets. Consistently, Rad60 and Slx8-Rfp mutants have similar DNA repair defects...
  43. pmc Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant
    Shital A Tripathi
    Mascoma Corporation, Lebanon, NH 03755, USA
    Appl Environ Microbiol 76:6591-9. 2010
    ..thermocellum, a microbe that holds an exciting and promising future in the biofuel industry and development of sustainable energy resources...
  44. pmc DJ-1 gene deletion reveals that DJ-1 is an atypical peroxiredoxin-like peroxidase
    Eva Andres-Mateos
    Institute for Cell Engineering, Johns Hopkins University School of Medicine, 733 North Broadway, Suite 731, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 104:14807-12. 2007
    ..Taken together these data indicate that the DJ-1 KO mice have a deficit in scavenging mitochondrial H(2)O(2) due to the physiological function of DJ-1 as an atypical peroxiredoxin-like peroxidase...
  45. ncbi Sphingosine 1-phosphate receptors in health and disease: mechanistic insights from gene deletion studies and reverse pharmacology
    Volker Brinkmann
    Autoimmunity and Transplantation, Novartis Institutes for BioMedical Research, Lichtstrasse 35, CH 4002 Basel, Switzerland
    Pharmacol Ther 115:84-105. 2007
    ..b>Gene deletion studies and reverse pharmacology (techniques aiming to identify both ligands and function of receptors) ..
  46. pmc Identification and characterization of two novel proteins affecting fission yeast gamma-tubulin complex function
    Srinivas Venkatram
    Department of Cell and Developmental Biology, Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Mol Biol Cell 15:2287-301. 2004
    ..This study expands the known roles of gamma-tubulin complex components in organizing different types of microtubule structures in S. pombe...
  47. ncbi Myc deletion rescues Apc deficiency in the small intestine
    Owen J Sansom
    The Beatson Institute, Garscube Estate, Glasgow G61 1BD, UK
    Nature 446:676-9. 2007
    ..Array analysis revealed that Myc is required for the majority of Wnt target gene activation following Apc loss. These data establish Myc as the critical mediator of the early stages of neoplasia following Apc loss...
  48. ncbi Dissection of a type VI secretion system in Edwardsiella tarda
    Jun Zheng
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543
    Mol Microbiol 66:1192-206. 2007
    ..The interaction between EvpC and EvpP was demonstrated. Lastly, two proteins (EvpD and EvpJ) are not required for the T6SS-dependent secretion...
  49. ncbi Eya protein phosphatase activity regulates Six1-Dach-Eya transcriptional effects in mammalian organogenesis
    Xue Li
    Howard Hughes Medical Institute, School and Department of Medicine, UCSD, 9500 Gilman Drive, Room 345, La Jolla, California 92093 0648, USA
    Nature 426:247-54. 2003
    ....
  50. ncbi Deciphering the effects of gene deletion on yeast longevity using network and machine learning approaches
    Tao Huang
    Key Laboratory of Systems Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, People s Republic of China
    Biochimie 94:1017-25. 2012
    ..Moreover, it has not escaped our notice that, after some modification, the current model can also be used to study many other phenotype prediction problems from the angle of systems biology...
  51. pmc Improving the MVA vaccine potential by deleting the viral gene coding for the IL-18 binding protein
    Juliana Falivene
    Centro Nacional de Referencia para el SIDA, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
    PLoS ONE 7:e32220. 2012
    ..The aim of this study was to evaluate cellular immune responses (CIR) induced by an IL-18 binding protein gene (C12L) deleted vector (MVAΔC12L)...
  52. pmc Systematic genetic analysis of virulence in the human fungal pathogen Cryptococcus neoformans
    Oliver W Liu
    Department of Biochemistry and Biophysics, University of California, San Francisco, 600 16th St, GH N372C, San Francisco, CA 94158, USA
    Cell 135:174-88. 2008
    ..In particular, we describe a GATA family transcription factor that inhibits phagocytosis by murine macrophages independently of the capsule, indicating a previously unknown mechanism of innate immune modulation...
  53. ncbi Comprehensive phenotypic analysis of single-gene deletion and overexpression strains of Saccharomyces cerevisiae
    Katsunori Yoshikawa
    Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1 5 Yamadaoka, Suita, Osaka, Japan
    Yeast 28:349-61. 2011
    We quantified the growth behaviour of all available single-gene deletion and overexpression strains of budding yeast...
  54. ncbi Endonuclease G regulates budding yeast life and death
    Sabrina Buttner
    Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria
    Mol Cell 25:233-46. 2007
    ..Deletion of NUC1 diminishes apoptotic death when mitochondrial respiration is increased but enhances necrotic death when oxidative phosphorylation is repressed, pointing to dual--lethal and vital--roles for EndoG...
  55. pmc Indian hedgehog signaling regulates proliferation and differentiation of chondrocytes and is essential for bone formation
    B St-Jacques
    Harvard University, Department of Molecular and Cellular Biology, Cambridge, Massachusetts 02138 USA
    Genes Dev 13:2072-86. 1999
    ..Together, the results suggest a model in which Ihh coordinates diverse aspects of skeletal morphogenesis through PTHrP-dependent and independent processes...
  56. ncbi Lymphocyte egress from thymus and peripheral lymphoid organs is dependent on S1P receptor 1
    Mehrdad Matloubian
    Howard Hughes Medical Institute and Department of Microbiology, University of California San Francisco, 513 Parnassus Avenue, San Francisco, California 94143 0414, USA
    Nature 427:355-60. 2004
    ..These findings establish that S1P1 is essential for lymphocyte recirculation and that it regulates egress from both thymus and peripheral lymphoid organs...
  57. ncbi Prediction of deleterious human alleles
    S Sunyaev
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany
    Hum Mol Genet 10:591-7. 2001
    ..The average human genotype carries approximately 10(3) damaging non-synonymous SNPs that together cause a substantial reduction in fitness...
  58. pmc Transcriptional regulation of chemical diversity in Aspergillus fumigatus by LaeA
    Robyn M Perrin
    Department of Plant Pathology, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS Pathog 3:e50. 2007
    ..Our findings suggest that LaeA is a novel target for comprehensive modification of chemical diversity and pathogenicity...
  59. pmc veA is required for toxin and sclerotial production in Aspergillus parasiticus
    Ana M Calvo
    Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA
    Appl Environ Microbiol 70:4733-9. 2004
    ....
  60. ncbi Monoallelically expressed gene related to p53 at 1p36, a region frequently deleted in neuroblastoma and other human cancers
    M Kaghad
    Sanofi Recherche, Labege, France
    Cell 90:809-19. 1997
    ..We propose that the disregulation of p73 contributes to tumorigenesis and that p53-related proteins operate in a network of developmental and cell cycle controls...
  61. ncbi Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells
    In Kyung Park
    Division of Hematology Oncology, Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109, USA
    Nature 423:302-5. 2003
    ..Expression of p16Ink4a and p19Arf in normal HSCs resulted in proliferative arrest and p53-dependent cell death, respectively. Our results indicate that Bmi-1 is essential for the generation of self-renewing adult HSCs...
  62. pmc Tetracycline-inducible gene expression and gene deletion in Candida albicans
    Yang Nim Park
    Institut für Molekulare Infektionsbiologie, Universitat Wurzburg, Rontgenring 11, D 97070 Wurzburg, Germany
    Eukaryot Cell 4:1328-42. 2005
    ..albicans and provides a convenient, versatile new tool to study gene function and manipulate cellular behavior in this model pathogenic fungus...
  63. ncbi De novo DNA cytosine methyltransferase activities in mouse embryonic stem cells
    H Lei
    Cardiovascular Research Center, Massachusetts General Hospital East, Charlestown 02129, USA
    Development 122:3195-205. 1996
    ..These results provide the first evidence that an independently encoded DNA methyltransferase is present in mammalian cells which is capable of de novo methylating cellular and viral DNA in vivo...
  64. ncbi Global mapping of the yeast genetic interaction network
    Amy Hin Yan Tong
    Banting and Best Department of Medical Research, University of Toronto, Toronto, ON, Canada M5G 1L6
    Science 303:808-13. 2004
    ..Because digenic interactions are common in yeast, similar networks may underlie the complex genetics associated with inherited phenotypes in other organisms...
  65. pmc Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization
    A J Link
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 179:6228-37. 1997
    ..coli. Because these strains are isogenic with the exception of their deleted ORFs, they may be used in competition with one another to reveal phenotypes not apparent when cultured singly...
  66. ncbi A hybrid micro-macroevolutionary approach to gene tree reconstruction
    Dannie Durand
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    J Comput Biol 13:320-35. 2006
    ..It also offers a command line option that enables high-throughput analysis of a large number of trees...
  67. pmc Development of additional selectable markers for the halophilic archaeon Haloferax volcanii based on the leuB and trpA genes
    Thorsten Allers
    Institute of Genetics, University of Nottingham, Queen s Medical Centre, Nottingham NG7 2UH, United Kingdom
    Appl Environ Microbiol 70:943-53. 2004
    ..We constructed both integrative and replicative plasmids carrying the leuB or trpA gene under control of a constitutive promoter. The use of these selectable markers in deletion of the lhr gene of H. volcanii is described...
  68. pmc Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans
    R F Ketting
    The Hubrecht Laboratory and Center for Biomedical Genetics, Uppsalalaan 8, Utrecht, The Netherlands
    Genes Dev 15:2654-9. 2001
    ..dcr-1 mutants show a defect in RNAi. Furthermore, a combination of phenotypic abnormalities and RNA analysis suggests a role for dcr-1 in a regulatory pathway comprised of small temporal RNA (let-7) and its target (e.g., lin-41)...
  69. pmc Genome-wide analysis of syntenic gene deletion in the grasses
    James C Schnable
    Department of Plant and Microbial Biology, University of California Berkeley, CA, USA
    Genome Biol Evol 4:265-77. 2012
    ....
  70. ncbi Regulation of yeast replicative life span by TOR and Sch9 in response to nutrients
    Matt Kaeberlein
    Departments of Genome Sciences and Medicine, University of Washington, Seattle, WA 98195, USA
    Science 310:1193-6. 2005
    ..We propose that the TOR and Sch9 kinases define a primary conduit through which excess nutrient intake limits longevity in yeast...
  71. pmc Deletion of IKZF1 and prognosis in acute lymphoblastic leukemia
    Charles G Mullighan
    Department of Pathology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    N Engl J Med 360:470-80. 2009
    ..Recent genomewide analyses have identified a high frequency of DNA copy-number abnormalities in ALL, but the prognostic implications of these abnormalities have not been defined...
  72. pmc TMPRSS2-ERG gene fusion is not associated with outcome in patients treated by prostatectomy
    Anuradha Gopalan
    Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Cancer Res 69:1400-6. 2009
    ....
  73. pmc Pax6 activity in the lens primordium is required for lens formation and for correct placement of a single retina in the eye
    R Ashery-Padan
    Max Planck Institute of Biophysical Chemistry, Department of Molecular Cell Biology, Goettingen D 37077, Germany
    Genes Dev 14:2701-11. 2000
    ....
  74. pmc Human genomic deletions mediated by recombination between Alu elements
    Shurjo K Sen
    Department of Biological Sciences, Louisiana State University, Baton Rouge, 70803, USA
    Am J Hum Genet 79:41-53. 2006
    ..Overall, we found that Alu recombination-mediated genomic deletion has had a much higher impact than was inferred from previously identified isolated events and that it continues to contribute to the dynamic nature of the human genome...
  75. ncbi Spatial and temporal expression of the Cre gene under the control of the MMTV-LTR in different lines of transgenic mice
    K U Wagner
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198 6805, USA
    Transgenic Res 10:545-53. 2001
    Cre-loxP based gene deletion approaches hold great promise to enhance our understanding of molecular pathways controlling mammary development and breast cancer...
  76. pmc Conditional ablation of beta1 integrin in skin. Severe defects in epidermal proliferation, basement membrane formation, and hair follicle invagination
    S Raghavan
    Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637, USA
    J Cell Biol 150:1149-60. 2000
    ..This, in turn, is required for hemidesmosome stability, epidermal proliferation, and hair follicle morphogenesis. However, beta1 downregulation does not provide the trigger to terminally differentiate...
  77. pmc Urease expression by Cryptococcus neoformans promotes microvascular sequestration, thereby enhancing central nervous system invasion
    Michal A Olszewski
    Veteran s Administration Medical Center Ann Arbor, Ann Arbor, Michigan, USA
    Am J Pathol 164:1761-71. 2004
    ..neoformans...
  78. pmc Candida albicans Hap43 is a repressor induced under low-iron conditions and is essential for iron-responsive transcriptional regulation and virulence
    Po Chen Hsu
    Institute of Molecular and Cellular Biology, National Tsing Hua University, No 101, Section 2, Kuang Fu Road, Hsinchu 30013, Taiwan, Republic of China
    Eukaryot Cell 10:207-25. 2011
    ..Taken together, our data suggest a complex interplay among Hap43, Sfu1, and Tup1 to coordinately regulate iron acquisition, iron utilization, and other iron-responsive metabolic activities...
  79. pmc Targeting of alpha-hemolysin by active or passive immunization decreases severity of USA300 skin infection in a mouse model
    Adam D Kennedy
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    J Infect Dis 202:1050-8. 2010
    ..We conclude that Hla is a potential target for therapeutics or vaccines designed to moderate severe S. aureus skin infections...
  80. ncbi Unmarked gene deletion and host-vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus
    Ling Deng
    Huazhong Agricultural University, Wuhan, People s Republic of China
    Extremophiles 13:735-46. 2009
    ..Two unmarked gene deletion methods were employed, namely plasmid integration and segregation, and marker replacement and looping out, and ..
  81. pmc Antimicrobial resistance to ceftazidime involving loss of penicillin-binding protein 3 in Burkholderia pseudomallei
    Narisara Chantratita
    Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand
    Proc Natl Acad Sci U S A 108:17165-70. 2011
    ..The mechanism described here represents an explanation for ceftazidime treatment failure, and may be a frequent but undetected resistance event...
  82. pmc Immunogenic profiling in mice of a HIV/AIDS vaccine candidate (MVA-B) expressing four HIV-1 antigens and potentiation by specific gene deletions
    Juan García-Arriaza
    Department of Molecular and Cellular Biology, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas CSIC, Madrid, Spain
    PLoS ONE 5:e12395. 2010
    ....
  83. ncbi Assessment of growth parameters and life span of GHR/BP gene-disrupted mice
    K T Coschigano
    Edison Biotechnology Institute, Ohio University, Athens 45701, USA
    Endocrinology 141:2608-13. 2000
    ..Finally, the -/- mice lived significantly longer than +/+ and +/- mice. The latter result contradicts the anti-aging GH data and suggests the need for further analysis of GH and aging...
  84. ncbi Inactivation of hCDC4 can cause chromosomal instability
    Harith Rajagopalan
    The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Nature 428:77-81. 2004
    ..Our data suggest that chromosomal instability is caused by specific genetic alterations in a large fraction of human cancers and can occur before malignant conversion...
  85. pmc Role of the Hog1 stress-activated protein kinase in the global transcriptional response to stress in the fungal pathogen Candida albicans
    Brice Enjalbert
    Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom
    Mol Biol Cell 17:1018-32. 2006
    ..albicans has diverged from corresponding networks in model yeasts and that the C. albicans SAPK pathway functions in parallel with other pathways to regulate the core transcriptional response to stress...
  86. ncbi Large-scale copy number polymorphism in the human genome
    Jonathan Sebat
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Science 305:525-8. 2004
    ..We observed copy number variation of 70 different genes within CNP intervals, including genes involved in neurological function, regulation of cell growth, regulation of metabolism, and several genes known to be associated with disease...
  87. ncbi Absence of S6K1 protects against age- and diet-induced obesity while enhancing insulin sensitivity
    Sung Hee Um
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland
    Nature 431:200-5. 2004
    ..Thus under conditions of nutrient satiation S6K1 negatively regulates insulin signalling...
  88. ncbi Mutations at the PAX6 locus are found in heterogeneous anterior segment malformations including Peters' anomaly
    I M Hanson
    MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK
    Nat Genet 6:168-73. 1994
    ..We therefore propose that a variety of anterior segment anomalies may be associated with PAX6 mutations...
  89. pmc Global systems-level analysis of Hfq and SmpB deletion mutants in Salmonella: implications for virulence and global protein translation
    Charles Ansong
    Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States of America
    PLoS ONE 4:e4809. 2009
    ....
  90. ncbi Quorum sensing controls biofilm formation in Vibrio cholerae
    Brian K Hammer
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
    Mol Microbiol 50:101-4. 2003
    ..Bacterial biofilms could represent a complex habitat where such differentiation occurs...
  91. pmc The N-terminal PIN domain of the exosome subunit Rrp44 harbors endonuclease activity and tethers Rrp44 to the yeast core exosome
    Claudia Schneider
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Nucleic Acids Res 37:1127-40. 2009
    ..Finally, the N-terminal PIN domain was shown to be necessary and sufficient for association with the core exosome, indicating its dual function as a nuclease and structural element...
  92. pmc Somatic LKB1 mutations promote cervical cancer progression
    Shana N Wingo
    Division of Gynecologic Oncology, UT Southwestern Medical Center, Dallas, TX, USA
    PLoS ONE 4:e5137. 2009
    ..Furthermore, LKB1 status can be exploited clinically to predict disease recurrence...
  93. pmc Rampant gene loss in the underground orchid Rhizanthella gardneri highlights evolutionary constraints on plastid genomes
    Etienne Delannoy
    Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Perth, Australia
    Mol Biol Evol 28:2077-86. 2011
    ..This prime example of convergent evolution implies shared selective constraints on gene loss or transfer...
  94. ncbi Dissection of phenotype reveals possible association between schizophrenia and Glutamate Receptor Delta 1 (GRID1) gene promoter
    Jens Treutlein
    Department of Genetic Epidemiology, Central Institute of Mental Health, J5, D 68159 Mannheim, Germany
    Schizophr Res 111:123-30. 2009
    ....
  95. pmc Liver-specific deletion of negative regulator Pten results in fatty liver and insulin hypersensitivity [corrected]
    Bangyan Stiles
    Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 101:2082-7. 2004
    ..Thus, deletion of Pten in the liver may provide a valuable model that permits the study of the metabolic actions of insulin signaling in the liver, and PTEN may be a promising target for therapeutic intervention for type 2 diabetes...
  96. ncbi Functions and regulation of the Nox family in the filamentous fungus Podospora anserina: a new role in cellulose degradation
    Sylvain Brun
    UFR des Sciences du Vivant, Univ Paris 7 Denis Diderot, 75013 Paris, France
    Mol Microbiol 74:480-96. 2009
    ..Importantly, as for similar structures present in some plant pathogens, their proper differentiation requires PaNox1, PaNox2, PaNoxR and the tetraspanin PaPls1...
  97. ncbi Toxicity of ligand-dependent Cre recombinases and generation of a conditional Cre deleter mouse allowing mosaic recombination in peripheral tissues
    Dorothe Hameyer
    Institute of Toxicology Mouse Genetics, Johannes Gutenberg Universitat Mainz, Mainz, Germany
    Physiol Genomics 31:32-41. 2007
    ....
  98. ncbi Evidence for autolysin-mediated primary attachment of Staphylococcus epidermidis to a polystyrene surface
    C Heilmann
    Institut fur Medizinische Mikrobiologie, Munster, Germany
    Mol Microbiol 24:1013-24. 1997
    ..Our findings provide evidence for a new function of an autolysin (AtlE) in mediating the attachment of bacterial cells to a polymer surface, representing the prerequisite for biofilm formation...
  99. ncbi A lecithin cholesterol acyltransferase-like gene mediates diacylglycerol esterification in yeast
    P Oelkers
    Institute of Human Nutrition, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Biol Chem 275:15609-12. 2000
    ..These mechanisms may also exist in mammalian cells...
  100. ncbi Xist-deficient mice are defective in dosage compensation but not spermatogenesis
    Y Marahrens
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Genes Dev 11:156-66. 1997
    ..Our results indicate that the Xist RNA is required for female dosage compensation but plays no role in spermatogenesis...
  101. pmc Identification of FleQ from Pseudomonas aeruginosa as a c-di-GMP-responsive transcription factor
    Jason W Hickman
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Mol Microbiol 69:376-89. 2008
    ..Our results show that FleQ is a new type of c-di-GMP binding protein that controls the transcriptional regulation of EPS biosynthesis genes in P. aeruginosa...

Research Grants72

  1. Infant Screening and Diagnosis of Duchenne Muscular Dys*
    Paul Fernhoff; Fiscal Year: 2007
    ..screen positive and will be tested by the Molecular Genetics Laboratory within the screening laboratory for gene deletion or duplication...
  2. Soluble Epoxide Hydrolase as a Target for Ischemic Cardioprotection
    Donna M Van Winkle; Fiscal Year: 2012
    ..Four Specific Aims will be examined to determine: 1. if sEH inhibition and gene deletion is cardioprotective;2. if sEH cardioprotection is specifically mediated by EETs;3...
  3. The role of IKK beta in cardiomyocytes at baseline and following stress
    MATTHEW PETER COGGINS; Fiscal Year: 2013
    ..Two different gene deletion strategies were employed both Cre and an activatable Crefusion protein to generate both constitutive and ..
  4. Adiponectin and fetal programming
    Jianhua Shao; Fiscal Year: 2013
    ..Importantly, adiponectin gene deletion attenuated maternal obesity-induced high birthweight and placental LPL expression...
  5. CELLULAR REGULATION OF CLATHRIN ASSEMBLY AND FUNCTION
    Frances M Brodsky; Fiscal Year: 2013
    ..relative roles of the two vertebrate clathrin light chains LCa and LCb at the tissue level through targeted gene deletion from mice...
  6. Chronic Granulomatous Lung Inflammation Elicited by Carbon Nanotubes
    Mary Jane Thomassen; Fiscal Year: 2013
    ..will afford students opportunities to gain experience with animal models of lung disease, learn effects of gene deletion in disease, and interact with physicians to learn environmental causes of human lung disease and how human ..
  7. Role of NPC1L1 in regulating energy metabolism, nutrient utilization & weight gai
    Philip N Howles; Fiscal Year: 2010
    ..Our results also show that loss of NPC1L1 function, through ezetimibe treatment or gene deletion, protects mice from diet-induced obesity and insulin resistance...
  8. Selective Insulin Resistance- Diabetic Cardiovascular Diseases
    George L King; Fiscal Year: 2013
    ..EC) and apolipoprotein E (apoE-/-) exhibited significantly more atherosclerosis (2X) than apoE-/- single gene deletion mice in parallel with a 50% reduction in Akt/eNOS activation...
  9. MLK3 in metabolic stress signaling
    Anja Jaeschke; Fiscal Year: 2013
    ..a major source of metabolic stress that induce activation of the c-Jun NH2-terminal kinase (JNK) pathway, and gene deletion and pharmacological inhibition have established an important role for the JNK signaling pathway in obesity-..
  10. Hepatic CYP enzymes and host-pathogen interactions
    Edward T Morgan; Fiscal Year: 2013
    ..strategy for propagation and survival, the effects will be studied of Cyp4A10-/- and Cyp4A14-/- gene deletion on the pathologies associated with C. rodentium infection...
  11. Gq-coupled Receptors Inhibit PI 3-kinase/Akt Signaling Pathway
    Richard Z Lin; Fiscal Year: 2012
    ..it has not been possible to investigate the biological roles of these enzymes using whole‐body gene deletion due to embryonic lethality...
  12. Genomic profiling of yeast resistance to AFB1, a P450-activated carcinogen
    MICHAEL THOMAS FASULLO; Fiscal Year: 2013
    ..Genomic phenotyping using the ~5,000 yeast single-gene deletion haploid and diploid strains have been highly successful in determining genes that confer resistance to ..
  13. ULTRASTRUCTURE OF MESOLIMBIC TRANSMITTER INTERACTIONS
    Virginia M Pickel; Fiscal Year: 2012
    ..In this renewal grant application, electron microscopic immunolabeling (rat and mouse), and spatial-temporal gene deletion of the essential NR1 subunit of the NMDA receptor (mouse) will be used to answer fundamental question ..
  14. Pleiotropic Transcription Factors As Target For Intracerebral Hemorrhage Treatmen
    Jaroslaw A Aronowski; Fiscal Year: 2012
    ..after ICH in rodents reduce oxidative stress, inflammation, and protect brain cells from damage, while Nrf2 gene deletion has the opposite effect;(2) Hematoma resolution: Activators of Nrf2 promote phagocytosis by brain microglia/..
  15. Endothelial Aromatase in Sex-Specific Cerebrovascular Dysfunction After Ischemia
    KRISTEN LEANNE ZULOAGA; Fiscal Year: 2012
    ..In female mice, both aromatase gene deletion and pharmacological inhibition lead to worse outcome following cerebral ischemia, suggesting that aromatase ..
  16. Arrestin Regulation of CRF1 Signaling: Mechanisms for Stress Resilience and PTSD
    Richard L Hauger; Fiscal Year: 2013
    ..We have demonstrated that a [unreadable]arrestin2 gene deletion in CRF-R1-expressing cells induces a 5-fold increase in the maximum for CRF-stimulated cyclic AMP accumulation ..
  17. TRANSPLANTATION OF TESTIS STEM CELLS IN LARGE ANIMALS
    Ina Dobrinski; Fiscal Year: 2013
    ..an efficient system for isolation and expansion of porcine GSCs;2) to generate pigs carrying a targeted gene deletion through germ cell transplantation;3) to study the effects of the testicular microenvironment on morphogenesis ..
  18. Assay Development for Inhibitors of KSHV
    Paul M Lieberman; Fiscal Year: 2010
    ..Disruption of LANA by siRNA depletion or gene deletion blocks viral genome persistence during latent infection...
  19. Sp7 Mediated Control of Runx2 Function for Osteoblast Differentiation
    Amjad Javed; Fiscal Year: 2013
    ..Runx2 is an essential transcription factor for chondrocyte and osteoblast differentiation. Runx2 gene deletion results in complete failure of skeleton formation and embryonic lethality...
  20. MYC ONCOPROTEIN FUNCTION
    ROBERT NEIL EISENMAN; Fiscal Year: 2013
    ..The broad developmental role of mSin3A will be assessed through targeted gene deletion in mice...
  21. Regulation of Obesity by the Alpha-arrestin ARRDC3
    Ronald B Myers; Fiscal Year: 2013
    ..Specifically, I will use mice with tissue-specific gene deletion of Arrdc3 in white adipose tissue, and measure adiposity and energy expenditure in a diet-induced obesity ..
  22. Treatment for Dysmyelination in PMD and SPG2
    Pamela E Knapp; Fiscal Year: 2013
    ..Interestingly, complete gene deletion results in a milder neurologic impairment...
  23. Estrogen signaling transgenic mouse models
    Richard R Behringer; Fiscal Year: 2011
    ..The essential physiological functions of both ERs have been examined by gene deletion studies. However, few studies have investigated the in vivo consequences of ER over-expression...
  24. The population genetics of antibiotic resistance in multi-drug environments
    Roy Kishony; Fiscal Year: 2013
    ..between growth and resistance, we will use a robust drug gradient assay to screen genomic libraries of gene deletion and over-expression strains for increased drug resistance and evaluate whether this increased resistance comes ..
  25. Chemical-genetic functional annotation of the genome of a meningitis pathogen
    Hiten D Madhani; Fiscal Year: 2013
    ..to the dissection of fundamental eukaryotic cellular processes, the generation and analysis of analogous gene deletion collections in pathogenic fungi is now beginning to allow the systematic elucidation of the molecular ..
  26. A Mouse model of Barth syndrome, a mitochondrial cardiolipin disorder
    Zaza Khuchua; Fiscal Year: 2013
    ..function, these systems cannot be adequately used and manipulated to study the long range effects of gene deletion or mutation in the whole organism and do not allow development and testing of potential therapies...
  27. Regulation of Renal Response to Vasopressin by Glycogen Synthase
    Reena Rao; Fiscal Year: 2013
    ..to the progression of polycystic kidney disease, this aim will also test the hypothesis that inhibition or gene deletion of GSK3 can reduce cystogenesis...
  28. Cryptococcus neoformans Gene Knockout Resource
    Hiten D Madhani; Fiscal Year: 2013
    ..fungi such as Saccharomyces cerevisiae and Schizosaccharomyces pombe, the generation of analogous complete gene deletion collections in pathogenic fungi would create experimental tools to systematically elucidate the basis of ..
  29. Discovery of genes that extend yeast lifespan: Aging in immobilized cell reactors
    RAPHAEL ROSENZWEIG; Fiscal Year: 2009
    ..We will exploit this system as a tool to discover whether lifespan extension is possible under calorically non- restrictive conditions, and if so, we aim to discover underlying genetic determinants. ..
  30. Regulation of yeast cellular aging through chromatin and novel pathways
    Weiwei Dang; Fiscal Year: 2013
    ..The yeast deletion library, a collection of yeast strains, each harboring a single gene deletion, has been a tremendously valuable resource for defining gene function, understanding the structure and logic ..
  31. The use of genetic models to define the role of beta-catenin in post-natal growth
    REGIS J O'KEEFE; Fiscal Year: 2011
    ..development and uses an innovative approach that permits us to temporally control tissue specific conditional gene deletion in mice...
  32. PATHOGENESIS OF DIABETIC NEPHROPATHY
    Yashpal S Kanwar; Fiscal Year: 2013
    ..AIM III is to determine if MIOX gene deletion leads to amelioration in renal dysfunctions and progression to tubulo-interstitial injury in STZ-induced ..
  33. Osteoblast and Odontoblast Specific Regulatory Action of Runx2 for Bone and Tooth
    MITRA D ADHAMI; Fiscal Year: 2013
    ..We have previously confirmed this as a valid model for Runx2 gene ablation. For cell-type specific gene deletion, I will use the 2.3kb Type I Collagen promoter to drive Cre expression...
  34. Neural Plasticity During Acclimatization to Hypoxia
    Frank L Powell; Fiscal Year: 2013
    ..Then we will measure those molecular signals in mice after CSH and use pharmacology and conditional gene deletion to test their physiological significance for ventilatory acclimatization to CSH...
  35. DYNAMICS OF ASSEMBLY OF BONE MATRIX PROTEINS
    Sarah L Dallas; Fiscal Year: 2013
    ..In the previous funding cycle, using fibronectin null cell culture models and targeted gene deletion in osteoblasts, it was shown that fibronectin is essential for assembly of multiple bone ECM components, ..
  36. BTK SIGNALING IN B CELL SURVIVAL BY BAFF-R
    Wasif N Khan; Fiscal Year: 2011
    ..The significance of BAFF-R/Btk pathway in mature B cells will be assessed using mice with floxed Btk gene deletion (Aim 2)...
  37. Oxidative Stress and Glutamate Excitotoxicity on Aged Dopaminergic Neruons
    Heather A Boger; Fiscal Year: 2010
    ..However, it is not known whether a partial gene deletion of GDNF impacts subthalamic nucleus glutamate release into the substantia nigra and oxidative stress with age...
  38. Oxidative Stress and Glutamate Excitotoxicity on Aged Dopaminergic Neruons
    Heather A Boger; Fiscal Year: 2013
    ..However, it is not known whether a partial gene deletion of GDNF impacts subthalamic nucleus glutamate release into the substantia nigra and oxidative stress with age...
  39. Calcium-Sensing Receptors and Growth Plate Development
    Wenhan Chang; Fiscal Year: 2012
    ..AIMs: (1) to determine the role of the CaR at specific stages of growth plate development by inducing CaR gene deletion with Tam at timed intervals before and after birth;(2) to determine whether a high [Ca2+]e alters chondrocyte ..
  40. CYP1B1: A Molecular Target for Chemoprevention of Lung Adenocarcinoma
    Margie L Clapper; Fiscal Year: 2013
    ..Inhibition of CYP1B1 will be achieved by gene deletion or administration of 2,3',4,5'-tetramethoxystilbene (TMS), a synthetic analog of resveratrol that is ..
  41. Yeast Ess1, a Conserved PPlase Essential for Mitosis
    Steven D Hanes; Fiscal Year: 2010
    ..However, their normal function in cells is poorly understood, in part, because most can be removed by gene deletion in their respective organism without observable consequences...
  42. GABAergic regulation of glutamine synthetase and its role in preventing epilepsy
    Stephen J Moss; Fiscal Year: 2013
    ..The significance of GS for brain function has been revealed by the use of specific inhibitors and gene deletion. These manipulations lead to seizures and death that result from decreased synaptic inhibition...
  43. Atg6 in Protection Against Insulin Resistance
    NICHOLAS PERRY GREENE; Fiscal Year: 2012
    ..mitochondrial uncoupler CCCP-induced growth arrest;2) both saturated fatty acid feeding and heterozygous Atg6 gene deletion lead to accumulation of damaged mitochondria in drosophila indirect flight muscles;and 3) Atg6+/- mice appear ..
  44. Modulation of host innate immune response by Francisella tularensis
    Chandra Shekhar Bakshi; Fiscal Year: 2013
    ..By generating point and multiple gene deletion mutants of a close surrogate of Ft SchuS4- the live vaccine strain (LVS), we have established that Ft ..
  45. Systematic Phenotypic Analysis of the Gram-positive Envelope
    Jason M Peters; Fiscal Year: 2013
    ..approach to investigate envelope function in the Gram-negative bacterium Escherichia coli by exposing a gene deletion library to a panel of environmental stressors that targeted the envelope...
  46. Estrogen signaling transgenic mouse models
    Richard R Behringer; Fiscal Year: 2012
    ..The essential physiological functions of both ERs have been examined by gene deletion studies. However, few studies have investigated the in vivo consequences of ER over-expression...
  47. The Role of Ghrelin to Regulate Insulin Secretion in Health and Diabetes.
    Jenny Tong; Fiscal Year: 2013
    ..Glucose tolerance and insulin secretion will be compared in mice with a global GHSR gene deletion and animals with GHSR only on beta cells or only in the nervous system. 2...
  48. Role of Intra-Pancreatic Trypsinogen Activation in Alcoholic Pancreatitis
    Rajinder K Dawra; Fiscal Year: 2013
    ..Cathepsin B activity is required for intra-acinar activation of trypsinogen and the mice with cathepsin B gene deletion also lack pathological trypsinogen activation...
  49. MMP14-dependent regulation of adipose tissue remodeling and function (R01)
    Tae Hwa Chun; Fiscal Year: 2013
    ..Using a peri-adipocyte fibrosis model, i.e., tamoxifen-inducible adipocyte-selective MMP14 gene deletion, I will characterize the link between peri-adipocyte fibrosis, inflammatory tissue damage, and the induction ..
  50. The Role of Microglia and the Kynurenine Pathway in Huntington's Disease
    Paul J Muchowski; Fiscal Year: 2012
    ..We recently completed a loss-of-function (LOF) genomic screen in S. cerevisiae with single gene deletion strains that identified kynurenine 3-monooxygenase (KMO), an enzyme in the KP of tryptophan degradation, as a ..
  51. Neutrophil-epithelial interaction mediated by adenosine
    Didier Merlin; Fiscal Year: 2013
    ..IL-8 secretion and mediates neutrophil recruitment in response to inflammatory stimuli;v) A2BAR antagonism or gene deletion inhibits neutrophil infiltration;vi) genetic ablation of A2BAR in mice protects against bacterial and ..
  52. Phenotypic Plasticity and Inter-Species Communication in Candida Albicans
    STEPHEN KNOX JONES; Fiscal Year: 2013
    ..I hypothesize that the thioredoxin pathway and Rad53 pathways are involved, so they will be tested using gene deletion and over-expression strains for TRX1, TRR1, CAP1, and RAD53...
  53. Babesiosis: An Emerging Infectious Threat to Transfusion Medicine
    Cheryl Ann Lobo; Fiscal Year: 2013
    ..native and recombinant Bd30 ligand protein and antibodies against Bd30 in invasion assays, as well as proposed gene deletion to study its function...
  54. Dystrophin Function in Aging Heart
    JOSEPH MARK METZGER; Fiscal Year: 2013
    ..Hypothesis: temporal dystrophin gene deletion will cause cardiac injury and heart performance deficits with effects greater in old compared to young animals. ..
  55. TOR, Translation and Aging
    Brian K Kennedy; Fiscal Year: 2013
    ..As a result of a genome-wide screen for long-lived yeast gene deletion strains, we identified a number of ribosomal large subunit gene deletions that result in reduced 60S subunit ..
  56. Mechanisms How Adipocytes Affect Bone in Aging Mice
    Fredric B Kraemer; Fiscal Year: 2010
    ..affect the micro-environment of the bone marrow and that removal of hormone-sensitive lipase function by gene deletion maintains high bone mass in aging mice;and 3) to identify and establish the relative importance of adipose-..
  57. Metabolic Cellular Stress and its Regulatory Mechanism in Diabetic Embryopathy
    Peixin Yang; Fiscal Year: 2012
    ..trehalose, reverses hyperglycemia-induced autophagy impairment and neural tube defects (NTDs);3) PKCa gene deletion, a p70S6K1 inhibitor, an ER chaperone (4-PBA) and overexpression of sirtuin 2 (SIRT2) histone deacetylase in ..
  58. Functional Characterization of Leishmania Transporters
    Scott M Landfear; Fiscal Year: 2010
    ..and determine which of them is/are impaired by when glucose transporters are genetically ablated by targeted gene deletion. These fates of glucose include: i) incorporation into glycoconjugates and complex carbohydrates, ii) ..
  59. DYNAMICS OF ASSEMBLY OF BONE MATRIX PROTEINS
    SARAH DALLAS; Fiscal Year: 2009
    ..In the previous funding cycle, using fibronectin null cell culture models and targeted gene deletion in osteoblasts, it was shown that fibronectin is essential for assembly of multiple bone ECM components, ..
  60. Oxalate Handling in PAT1 and DRA Knockout Mice
    Marguerite Hatch; Fiscal Year: 2010
    ..Thus, we have already demonstrated that a one-gene deletion results in dramatic changes in oxalate handling...
  61. Molecular Dissection of Reactive Astrogliosis: STAT3
    Michael V Sofroniew; Fiscal Year: 2012
    ..To do so we have developed conditional gene deletion or knockout technology (CKO) for astrocytes using the Cre/loxP system under regulation of the mouse GFAP ..
  62. Vascular Calcium Channel Expression in Hypertension
    Nancy J Rusch; Fiscal Year: 2012
    ..on delineated the mechanism of vascular CaL channel abnormalities in hypertensive mice to take advantage of gene deletion models...
  63. GENETICS OF DELAYED PUBERTY
    LAWRENCE LAYMAN; Fiscal Year: 2001
    ..In the hypogonadal mouse, hypogonadotropic hypogonadism is due to a GnRH gene deletion, but no GnRH gene mutations have been identified in humans with IHH...
  64. Induction of the blood-retinal barrier
    DAVID ANTONETTI; Fiscal Year: 2010
    ..important to the blood-retinal barrier since this claudin is largely restricted to the vasculature and gene deletion studies have demonstrated claudin-5 is an essential component of the blood-brain and blood-retinal barrier...
  65. DEPENDENCE OF P53 ON THIOL MAINTENANCE PROTEINS
    Gary Merrill; Fiscal Year: 2001
    ..Equally important, the experiments test a general approach for deriving oxidation-resistant proteins using yeast. ..
  66. GYRATE ATROPHY-MODEL FOR MOLECULAR STUDY OF EYE DISEASES
    George Inana; Fiscal Year: 1991
    ..The specific nature of each defect, be it gene deletion, rearrangement, point mutation or other changes will be identified...
  67. Inhibition of lupus nephritis in IRF-1 deficient mice
    Christopher Reilly; Fiscal Year: 2003
    ..IFN-gamma constitutes one of the most potent macrophage activating factors. Either IFN-7 or IFN-y receptor gene deletion modulates disease activity in lupus mice although other adverse effects in these genetic knockouts were noted...
  68. The Post-Transcriptional Regulation of Peripheral Myelin Protein 22 by MicroRNAs
    JONATHAN VERRIER; Fiscal Year: 2009
    ..Both duplication and gene deletion result in neuropathic phenotypes indicating the necessity for precise control over protein expression...
  69. An in vivo Gene Deletion System for Analyzing Temporal Requirement of the Dot/Icm
    Zhao Qing Luo; Fiscal Year: 2009
    ..We have successfully designed a Cre/loxP-based inducible in vivo gene deletion strategy suitable for examining the temporal requirement of specific protein(s)...
  70. GLYCOGEN METABOLISM AND SIGNAL TRANSDUCTION IN YEAST
    Peter Roach; Fiscal Year: 2005
    ..It incorporates genome wide analyses based on the availability in the near future of a complete gene deletion set of about5000 yeast strains systematically disrupted for individual genes...
  71. Role of Lipolytic Enzymes in Intestinal Lipid Transport
    David Hui; Fiscal Year: 2009
    ..Our results also showed that pancreatic triglyceride lipase (PTL) gene deletion only delays but does not affect total amount of triglyceride absorption, but significantly decreases both the ..
  72. BRUCELLA VACCINE FOR BIOTERRORISM
    Gary Splitter; Fiscal Year: 2003
    ..Specific Aim 1 will identify Brucella abortus genes that affect intracellular survival and then engineer those gene deletion mutants of Brucella...