reporter genes

Summary

Summary: Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.

Top Publications

  1. pmc Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
    S Srinivas
    Department of Genetics and Development, Columbia University, New York, USA
    BMC Dev Biol 1:4. 2001
  2. ncbi Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
    S M Elbashir
    Department of Cellular Biochemistry, Max-Planck-Institute for Biophysical Chemistry, , Germany
    Nature 411:494-8. 2001
  3. ncbi Stochastic gene expression in a single cell
    Michael B Elowitz
    Laboratory of Cancer Biology, Center for Studies in Physics and Biology, Rockefeller University, New York, NY 10021, USA
    Science 297:1183-6. 2002
  4. pmc Mapping Wnt/beta-catenin signaling during mouse development and in colorectal tumors
    Silvia Maretto
    Histology and Embryology Section, Department of Histology, Microbiology, and Medical Biotechnology, University of Padua, 35131 Padua, Italy
    Proc Natl Acad Sci U S A 100:3299-304. 2003
  5. ncbi GAL4 system in Drosophila: a fly geneticist's Swiss army knife
    Joseph B Duffy
    Department of Biology, Indiana University, 1001 E 3rd Street, Bloomington, IN 47405, USA
    Genesis 34:1-15. 2002
  6. ncbi Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo
    Bakhos A Tannous
    Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Mol Ther 11:435-43. 2005
  7. pmc MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms
    Yan Zeng
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 100:9779-84. 2003
  8. ncbi Identification and functional characterization of distinct critically important bone morphogenetic protein-specific response elements in the Id1 promoter
    Olexander Korchynskyi
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 277:4883-91. 2002
  9. ncbi Regulation of noise in the expression of a single gene
    Ertugrul M Ozbudak
    Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Genet 31:69-73. 2002
  10. ncbi MicroRNA-directed cleavage of HOXB8 mRNA
    Soraya Yekta
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
    Science 304:594-6. 2004

Detail Information

Publications366 found, 100 shown here

  1. pmc Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
    S Srinivas
    Department of Genetics and Development, Columbia University, New York, USA
    BMC Dev Biol 1:4. 2001
    ....
  2. ncbi Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
    S M Elbashir
    Department of Cellular Biochemistry, Max-Planck-Institute for Biophysical Chemistry, , Germany
    Nature 411:494-8. 2001
    ..Therefore, 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics...
  3. ncbi Stochastic gene expression in a single cell
    Michael B Elowitz
    Laboratory of Cancer Biology, Center for Studies in Physics and Biology, Rockefeller University, New York, NY 10021, USA
    Science 297:1183-6. 2002
    ..These results establish a quantitative foundation for modeling noise in genetic networks and reveal how low intracellular copy numbers of molecules can fundamentally limit the precision of gene regulation...
  4. pmc Mapping Wnt/beta-catenin signaling during mouse development and in colorectal tumors
    Silvia Maretto
    Histology and Embryology Section, Department of Histology, Microbiology, and Medical Biotechnology, University of Padua, 35131 Padua, Italy
    Proc Natl Acad Sci U S A 100:3299-304. 2003
    ..In summary, BAT-gal mice unveil the entire complexity of Wntbeta-catenin signaling in mammals and have broad application potentials for the identification of Wnt-responsive cell populations in development and disease...
  5. ncbi GAL4 system in Drosophila: a fly geneticist's Swiss army knife
    Joseph B Duffy
    Department of Biology, Indiana University, 1001 E 3rd Street, Bloomington, IN 47405, USA
    Genesis 34:1-15. 2002
  6. ncbi Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo
    Bakhos A Tannous
    Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Mol Ther 11:435-43. 2005
    ....
  7. pmc MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms
    Yan Zeng
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 100:9779-84. 2003
    ..These data suggest that miRNAs and siRNAs can use similar mechanisms to repress mRNA expression and that the choice of mechanism may be largely or entirely determined by the degree of complementary of the RNA target...
  8. ncbi Identification and functional characterization of distinct critically important bone morphogenetic protein-specific response elements in the Id1 promoter
    Olexander Korchynskyi
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 277:4883-91. 2002
    ..Our results provide important new insights into how the BMP/Smad pathway can specifically activate target genes...
  9. ncbi Regulation of noise in the expression of a single gene
    Ertugrul M Ozbudak
    Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Genet 31:69-73. 2002
    ..Our results thus provide the first direct experimental evidence of the biochemical origin of phenotypic noise, demonstrating that the level of phenotypic variation in an isogenic population can be regulated by genetic parameters...
  10. ncbi MicroRNA-directed cleavage of HOXB8 mRNA
    Soraya Yekta
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
    Science 304:594-6. 2004
    ....
  11. ncbi Constitutive transcriptional activation by a beta-catenin-Tcf complex in APC-/- colon carcinoma
    V Korinek
    Department of Immunology, University Hospital, Post Office Box 85500, 3508 GA Utrecht, Netherlands
    Science 275:1784-7. 1997
    ..Constitutive transcription of Tcf target genes, caused by loss of APC function, may be a crucial event in the early transformation of colonic epithelium...
  12. ncbi Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC
    P J Morin
    Howard Hughes Medical Institute and Johns Hopkins Oncology Center, 424 North Bond Street, Baltimore, MD 21231, USA
    Science 275:1787-90. 1997
    ..These results indicate that regulation of beta-catenin is critical to APC's tumor suppressive effect and that this regulation can be circumvented by mutations in either APC or beta-catenin...
  13. ncbi MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb
    Changchun Xiao
    The CBR Institute for Biomedical Research, Harvard Medical School, Boston, MA 02115, USA
    Cell 131:146-59. 2007
    ....
  14. pmc MicroRNA genes are transcribed by RNA polymerase II
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Science, Seoul National University, Seoul, Korea
    EMBO J 23:4051-60. 2004
    ..These data indicate that pol II is the main, if not the only, RNA polymerase for miRNA gene transcription. Our study offers a basis for understanding the structure and regulation of miRNA genes...
  15. ncbi Regulating gene expression through RNA nuclear retention
    Kannanganattu V Prasanth
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Cell 123:249-63. 2005
    ..This mechanism of action highlights an important paradigm for the role of a nuclear-retained stable RNA transcript in regulating gene expression...
  16. ncbi Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10021, USA
    Mol Cell 15:185-97. 2004
    ..The specific role of Ago2 in guiding target RNA cleavage was confirmed independently by siRNA-based depletion of individual Ago members in combination with a sensitive positive-readout reporter assay...
  17. pmc Understanding the function-structure and function-mutation relationships of p53 tumor suppressor protein by high-resolution missense mutation analysis
    Shunsuke Kato
    Department of Clinical Oncology, Institute of Development, Aging, and Cancer, Tohoku University, Sendai 980 8575, Japan
    Proc Natl Acad Sci U S A 100:8424-9. 2003
    ..This high-resolution mutation analysis allows evaluation of previous predictions and hypotheses through interrelation of function, structure and mutation...
  18. ncbi Intragenic DNA methylation alters chromatin structure and elongation efficiency in mammalian cells
    Matthew C Lorincz
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Nat Struct Mol Biol 11:1068-75. 2004
    ..As the methylated region adopts a closed chromatin structure in vivo, we propose that dense intragenic DNA methylation in mammalian cells initiates formation of a chromatin structure that reduces the efficiency of Pol II elongation...
  19. ncbi RAS is regulated by the let-7 microRNA family
    Steven M Johnson
    Department of Molecular, Cellular and Developmental Biology, Yale University, P O Box 208103, New Haven, CT 06520, USA
    Cell 120:635-47. 2005
    ..let-7 expression is lower in lung tumors than in normal lung tissue, while RAS protein is significantly higher in lung tumors, providing a possible mechanism for let-7 in cancer...
  20. ncbi PCR fusion-based approach to create reporter gene constructs for expression analysis in transgenic C. elegans
    Oliver Hobert
    Department of Biochemistry and Molecular Biophysics, Center for Neurobiology and Behavior, Columbia University, College of Physicians and Surgeons, New York, NY 10032, USA
    Biotechniques 32:728-30. 2002
  21. ncbi NG2 cells generate both oligodendrocytes and gray matter astrocytes
    Xiaoqin Zhu
    Department of Physiology and Neurobiology, University of Connecticut, Storrs, CT 06269 3156, USA
    Development 135:145-57. 2008
    ..These observations suggest that NG2+ cells are precursors of oligodendrocytes and some protoplasmic astrocytes in gray matter...
  22. ncbi Induction of ectopic eyes by targeted expression of the eyeless gene in Drosophila
    G Halder
    Biozentrum, University of Basel, Switzerland
    Science 267:1788-92. 1995
    ..Because homologous genes are present in vertebrates, ascidians, insects, cephalopods, and nemerteans, ey may function as a master control gene throughout the metazoa...
  23. ncbi Efficient gene transfer into the embryonic mouse brain using in vivo electroporation
    T Saito
    Department of Development and Differentiation, Kyoto University, Kyoto, 606 8507, Japan
    Dev Biol 240:237-46. 2001
    ..Moreover, it was possible to transfect three different DNA vectors into the same cells. Thus, this method will be a powerful tool to characterize gene function in various settings due to its high efficiency and localized gene expression...
  24. pmc Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway
    Eek Hoon Jho
    Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Mol Cell Biol 22:1172-83. 2002
    ..Our results suggest that Axin2 participates in a negative feedback loop, which could serve to limit the duration or intensity of a Wnt-initiated signal...
  25. ncbi Advances in in vivo bioluminescence imaging of gene expression
    Christopher H Contag
    Department of Pediatrics, Stanford University School of Medicine, California 94305 5208, USA
    Annu Rev Biomed Eng 4:235-60. 2002
    ..Such studies will greatly facilitate the functional analysis of a wide range of genes for their roles in health and disease...
  26. ncbi A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA
    E C Lee
    Mouse Cancer Genetics Program, National Cancer Institute Frederick, Frederick, Maryland 21702, USA
    Genomics 73:56-65. 2001
    ....
  27. pmc Emergence of resistant human immunodeficiency virus type 1 in patients receiving fusion inhibitor (T-20) monotherapy
    Xiping Wei
    Howard Hughes Medical Institute, Department of Medicine, University of Alabama at Birmingham, 35294, USA
    Antimicrob Agents Chemother 46:1896-905. 2002
    ..These findings provide the first evidence for the rapid emergence of clinical resistance to a novel class of HIV-1 entry inhibitors and may be relevant to future treatment strategies involving these agents...
  28. ncbi Notch-1 signalling requires ligand-induced proteolytic release of intracellular domain
    E H Schroeter
    Department of Molecular Biology and Pharmacology, Washington University, St Louis, Missouri 63110, USA
    Nature 393:382-6. 1998
    ..Very small amounts of NICD are active, explaining why it is hard to detect in the nucleus in vivo. We also show that it is ligand binding that induces release of NICD...
  29. ncbi Applications of bioluminescence imaging to the study of infectious diseases
    Martha Hutchens
    Immunology Program, University of Michigan Medical School, Ann Arbor, MI 48109 2200, USA
    Cell Microbiol 9:2315-22. 2007
    ..We expect that both the range of organisms and the molecular events able to be studied by BLI will continue to expand, yielding important insights into mechanisms of pathogenesis...
  30. ncbi Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity
    D P Silver
    The Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 8:233-43. 2001
    ....
  31. ncbi Fate of the mammalian cardiac neural crest
    X Jiang
    Departments of Biological Sciences and Cell and Neurobiology, Institute for Genetic Medicine, Keck School of Medicine, University of Southern California, IGM 240, Los Angeles, CA 90033, USA
    Development 127:1607-16. 2000
    ....
  32. ncbi Resetting central and peripheral circadian oscillators in transgenic rats
    S Yamazaki
    NSF Center for Biological Timing and Department of Biology, University of Virginia, Charlottesville, VA 22903-2477, USA
    Science 288:682-5. 2000
    ....
  33. pmc Myogenic specification of side population cells in skeletal muscle
    Atsushi Asakura
    Molecular Medicine Program, Ottawa Health Research Institute, Ottawa, Ontario, K1H 8L6 Canada
    J Cell Biol 159:123-34. 2002
    ....
  34. ncbi Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria
    S Kalir
    Department of Molecular Cell Biology, Department of Physics of Complex Systems, Weizmann Institute of Science, Rehovot 76100, Israel
    Science 292:2080-3. 2001
    ..The observed temporal program of transcription was much more detailed than was previously thought and was associated with multiple steps of flagella assembly...
  35. pmc A toolkit and robust pipeline for the generation of fosmid-based reporter genes in C. elegans
    Baris Tursun
    Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University Medical Center, New York, New York, United States of America
    PLoS ONE 4:e4625. 2009
    ..We describe other applications of fosmid recombineering as well. The simplicity, speed and robustness of the recombineering pipeline described here should prompt the routine use of this strategy for expression studies in C. elegans...
  36. ncbi Entrainment of the circadian clock in the liver by feeding
    K A Stokkan
    National Science Foundation Center for Biological Timing and Department of Biology, University of Virginia, P O Box 400328, Charlottesville, VA 22904 4328, USA
    Science 291:490-3. 2001
    ..They also raise the possibility that peripheral circadian oscillators like those in the liver may be coupled to the SCN primarily through rhythmic behavior, such as feeding...
  37. ncbi BDNF-expressing marrow stromal cells support extensive axonal growth at sites of spinal cord injury
    P Lu
    Department of Neurosciences and Center for Neural Repair, University of California at San Diego, La Jolla, CA 92093 0626, USA
    Exp Neurol 191:344-60. 2005
    ..Combination therapy with other experimental approaches will likely be required to achieve axonal growth beyond the lesion site and functional recovery...
  38. ncbi A gene expression atlas of the central nervous system based on bacterial artificial chromosomes
    Shiaoching Gong
    GENSAT Project, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, Box 260, New York 10021, USA
    Nature 425:917-25. 2003
    ..The atlas, library of BAC vectors and BAC transgenic mice generated in this screen provide a rich resource that allows a broad array of investigations not previously available to the neuroscience community...
  39. pmc Expression of the gene encoding the proapoptotic Nip3 protein is induced by hypoxia
    R K Bruick
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235 9152, USA
    Proc Natl Acad Sci U S A 97:9082-7. 2000
    ..These observations indicate that Nip3 may play a dedicated role in the pathological progression of hypoxia-mediated apoptosis, as observed after ischemic injury...
  40. pmc Assessment of immunity to mycobacterial infection with luciferase reporter constructs
    V A Snewin
    Department of Infectious Diseases and Microbiology, Imperial College School of Medicine, St Mary s Campus, London W2 1PG, United Kingdom
    Infect Immun 67:4586-93. 1999
    ....
  41. pmc Molecular mechanisms of gene silencing mediated by DNA methylation
    Michela Curradi
    Dipartimento di Biologia Strutturale e Funzionale, Universita degli Studi dell Insubria, 21052 Busto Arsizio VA, Italy
    Mol Cell Biol 22:3157-73. 2002
    ....
  42. pmc Bmp4 is required for the generation of primordial germ cells in the mouse embryo
    K A Lawson
    Hubrecht Laboratory, Netherlands Institute for Developmental Biology, 3584 CT Utrecht, The Netherlands
    Genes Dev 13:424-36. 1999
    ..The initiation of the germ line in the mouse therefore depends on a secreted signal from the previously segregated, extraembryonic, trophectoderm lineage...
  43. pmc siRNAs can function as miRNAs
    John G Doench
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 17:438-42. 2003
    ..The use of siRNAs to study translational repression holds promise for dissecting the sequence and structural determinants and general mechanism of gene repression by miRNAs...
  44. ncbi Effects of sod gene overexpression and deletion mutation on the expression profiles of reporter genes of major detoxification pathways in Caenorhabditis elegans
    Patricia Back
    Laboratory for Aging Physiology and Molecular Evolution, Department of Biology, Ghent University, K L Ledeganckstraat 35, Ghent, Belgium
    Exp Gerontol 45:603-10. 2010
    ..Overall, our results suggest that sod gene manipulation causes complex, combinatorial regulation of expression of individual targets of stress sensitive transcription factors...
  45. ncbi Kinetic analysis and modeling of firefly luciferase as a quantitative reporter gene in live mammalian cells
    Jolene M Ignowski
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, MC 1462, University of California Berkeley, Berkeley, CA 94720 1462, USA
    Biotechnol Bioeng 86:827-34. 2004
    ..Finally, we have modeled luciferase activity in live cells for the purposes of understanding and translating the luciferase signal into a more effective metric of gene expression and cell behavior...
  46. pmc A combined computational-experimental approach predicts human microRNA targets
    Marianthi Kiriakidou
    Department of Pathology, School of Medicine, Center for Bioinformatics, and Computer and Information Science, School of Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Genes Dev 18:1165-78. 2004
    ..We describe a computational program, "DIANA-microT", that identifies mRNA targets for animal miRNAs and predicts mRNA targets, bearing single MREs, for human and mouse miRNAs...
  47. ncbi Reporter gene technology: the future looks bright
    L H Naylor
    The Department of Biosciences, The University of Kent, Canterbury, UK
    Biochem Pharmacol 58:749-57. 1999
    ..Based upon the splicing of transcriptional control elements to a variety of reporter genes (with easily measurable phenotypes), it "reports" the effects of a cascade of signalling events on gene ..
  48. ncbi A cellular microRNA mediates antiviral defense in human cells
    Charles Henri Lecellier
    CNRS Unité Propre de Recherche UPR 2357, Institut de Biologie Moleculaire des Plantes, 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France
    Science 308:557-60. 2005
    ..Therefore, through fortuitous recognition of foreign nucleic acids, cellular microRNAs have direct antiviral effects in addition to their regulatory functions...
  49. pmc A mutant herpes simplex virus type 1 thymidine kinase reporter gene shows improved sensitivity for imaging reporter gene expression with positron emission tomography
    S S Gambhir
    The Crump Institute for Biological Imaging, University of California Department of Energy Laboratory of Structural Biology and Molecular Medicine, University of California School of Medicine, Los Angeles, CA 90095 1770, USA
    Proc Natl Acad Sci U S A 97:2785-90. 2000
    We are developing assays for noninvasive, quantitative imaging of reporter genes with positron emission tomography (PET), for application both in animal models and in human gene therapy...
  50. pmc Identification and cloning of unc-119, a gene expressed in the Caenorhabditis elegans nervous system
    M Maduro
    Department of Biological Sciences, University of Alberta, Edmonton, Canada
    Genetics 141:977-88. 1995
    ..Expression of an unc-119/lacZ fusion in transgenic animals is seen in many neurons, suggesting that the unc-119 mutant phenotype is due to a defect in the nervous system...
  51. ncbi Reporter genes facilitating discovery of drugs targeting protozoan parasites
    Anuradha Dube
    Division of Parasitology, Central Drug Research Institute, Lucknow 226 001, India
    Trends Parasitol 25:432-9. 2009
    ..Furthermore, reporter genes have proved to be an excellent and promising tool for studying disease progression...
  52. ncbi Observation of antigen-dependent CD8+ T-cell/ dendritic cell interactions in vivo
    B C Schaefer
    National Jewish Medical and Research Center, 1400 Jackson Street, K512, Denver, Colorado 80206, USA
    Cell Immunol 214:110-22. 2001
    ..Dendritic cells undergo specific morphologic changes following interactions with antigen-specific T cells...
  53. ncbi Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors
    Carlos Lois
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Science 295:868-72. 2002
    ..We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species...
  54. ncbi Role of basic region leucine zipper transcription factors cyclic AMP response element binding protein (CREB), CREB2, activating transcription factor 2 and CAAT/enhancer binding protein alpha in cyclic AMP response element-mediated transcription
    Gerald Thiel
    Department of Medical Biochemistry and Molecular Biology, University of Saarland Medical Center, Homburg, Germany
    J Neurochem 92:321-36. 2005
    ..In both cases, a striking stimulation of transcription of CRE-containing reporter genes was observed in noradrenergic locus coeruleus-like CATH.a cells...
  55. ncbi Transcriptional profiling of reporter genes used for molecular imaging of embryonic stem cell transplantation
    Joseph C Wu
    Division of Cardiology, Department of Medicine, Stanford University School of Medicine, Stanford, USA
    Physiol Genomics 25:29-38. 2006
    ..TF) construct consisting of fluorescence, bioluminescence, and positron emission tomography (PET) reporter genes. Fluorescence-activated cell sorting (FACS) analysis allowed isolation of stably transfected populations...
  56. ncbi Green fluorescent protein as a marker for gene expression and subcellular localization in budding yeast
    R K Niedenthal
    Institut fur Mikrobiologie und Molekularbiologie, Justus Liebig Universitat Giessen, Germany
    Yeast 12:773-86. 1996
    ..Systematic studies of a large number of genes should benefit from such assays...
  57. pmc Long term non-invasive imaging of embryonic stem cells using reporter genes
    Ning Sun
    Molecular Imaging Program at Stanford MIPS, Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA
    Nat Protoc 4:1192-201. 2009
    ..In this protocol, we describe the in vivo monitoring of stem cell survival, proliferation and migration using reporter genes. We established stable ES cell lines constitutively expressing double fusion (DF; enhanced green fluorescent ..
  58. ncbi Human high-density lipoprotein particles prevent activation of the JNK pathway induced by human oxidised low-density lipoprotein particles in pancreatic beta cells
    A Abderrahmani
    Service of Internal Medicine, CHUV Hospital, Rue du Bugnon 9, 1005 Lausanne, Switzerland
    Diabetologia 50:1304-14. 2007
    ..We explored the potential adverse effects of pro-atherogenic oxidised LDL-cholesterol particles on beta cell function...
  59. ncbi Delivery of short interfering RNA using endosomolytic cell-penetrating peptides
    Pontus Lundberg
    Department of Neurochemistry, Stockholm University, Svante Arrhenius Väg 21A, S 10691 Stockholm, Sweden
    FASEB J 21:2664-71. 2007
    ..We believe that developing CPPs with increased endosomolytical properties is a necessary step toward achieving biological effects at low concentrations for future in vivo applications...
  60. ncbi A tetracycline controlled activation/repression system with increased potential for gene transfer into mammalian cells
    S Freundlieb
    Zentrum für Molekulare Biologie ZMBH, Universitat Heidelberg, Germany
    J Gene Med 1:4-12. 1999
    ..g., during transient expression, intrinsic residual transcription persists in their 'off' state, which can differ in intensity among different cell types...
  61. ncbi Regulation of chondrocyte differentiation by Cbfa1
    I S Kim
    Universitäts Kinderklinik Mainz, Langenbeckstrasse 1, 55101, Mainz, Germany
    Mech Dev 80:159-70. 1999
    ..Our studies identify Cbfa1 as a major positive regulator of chondrocyte differentiation...
  62. pmc Post-integration stability of piggyBac in Aedes aegypti
    Nagaraja Sethuraman
    Center for Biosystems Research, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    Insect Biochem Mol Biol 37:941-51. 2007
    ..The post-integration mobility properties of piggyBac in Ae. aegypti enhance the utility of this gene vector for certain applications, particularly those where any level of vector remobilization is unacceptable...
  63. pmc Differentially expressed fibroblast growth factors regulate skeletal muscle development through autocrine and paracrine mechanisms
    K Hannon
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA
    J Cell Biol 132:1151-9. 1996
    ..Thus, production of FGF-1 and FGF-2 by skeletal muscle cells may act as a paracrine and autocrine regulator of skeletal muscle development in vivo...
  64. pmc Transgene Coplacement and high efficiency site-specific recombination with the Cre/loxP system in Drosophila
    M L Siegal
    Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Genetics 144:715-26. 1996
    ..The excision reactions of Cre/loxP and the related FLP/FRT system are used to create Drosophila lines in which transgenes are at exactly allelic sites in homologous chromosomes...
  65. ncbi ICAT inhibits beta-catenin binding to Tcf/Lef-family transcription factors and the general coactivator p300 using independent structural modules
    Danette L Daniels
    Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Mol Cell 10:573-84. 2002
    ..The C-terminal armadillo repeats of beta-catenin may be an attractive target for compounds designed to disrupt aberrant beta-catenin-mediated transcription associated with various cancers...
  66. ncbi Potent estrogenic metabolites of bisphenol A and bisphenol B formed by rat liver S9 fraction: their structures and estrogenic potency
    Shin Ichi Yoshihara
    Department of Xenobiotic Metabolism and Molecular Toxicology, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1 2 3, Minami Ku, Hiroshima 734 8551, Japan
    Toxicol Sci 78:50-9. 2004
    ....
  67. ncbi Red- and green-emitting firefly luciferase mutants for bioluminescent reporter applications
    Bruce R Branchini
    Department of Chemistry, Connecticut College, New London, CT 06320, USA
    Anal Biochem 345:140-8. 2005
    ..The results presented here with the P. pyralis mutants provide evidence that simultaneous multiple analyte assay development is feasible with these novel proteins that require only a single substrate...
  68. pmc Autoregulation of FCA pre-mRNA processing controls Arabidopsis flowering time
    Victor Quesada
    Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK
    EMBO J 22:3142-52. 2003
    ..The negative autoregulation of FCA may therefore have evolved to limit FCA activity and hence control flowering time...
  69. ncbi Aniridia-associated translocations, DNase hypersensitivity, sequence comparison and transgenic analysis redefine the functional domain of PAX6
    D A Kleinjan
    MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK
    Hum Mol Genet 10:2049-59. 2001
    ..Components of this downstream regulatory region drive reporter expression in distinct partial PAX6 patterns, indicating that the functional PAX6 gene domain extends far beyond the transcription unit...
  70. pmc Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2
    V Sudakin
    Institute for Cancer Research, The Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    J Cell Biol 154:925-36. 2001
    ..We propose that the preformed interphase pool of MCC allows for rapid inhibition of APC/C when cells enter mitosis. Unattached kinetochores then target the APC/C for sustained inhibition by the MCC...
  71. pmc MRI reporter genes
    Assaf A Gilad
    Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Nucl Med 49:1905-8. 2008
    Noninvasive molecular imaging of dynamic processes has benefited tremendously from the use of reporter genes. These genes encode for proteins that emit light, bind radiolabeled probes, or, as covered in this review, modulate MRI contrast...
  72. pmc Assigning numbers to the arrows: parameterizing a gene regulation network by using accurate expression kinetics
    Michal Ronen
    Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 99:10555-60. 2002
    ..This finding opens the possibility of assigning kinetic parameters to transcriptional networks on a genomic scale...
  73. ncbi OAZ uses distinct DNA- and protein-binding zinc fingers in separate BMP-Smad and Olf signaling pathways
    A Hata
    Cell Biology Program, Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Cell 100:229-40. 2000
    ..The mutually exclusive use of OAZ by the BMP-Smad and Olf pathways illustrates the dual role of a multi-zinc finger protein in signal transduction during development...
  74. ncbi A Plasmodium berghei reference line that constitutively expresses GFP at a high level throughout the complete life cycle
    Blandine Franke-Fayard
    Department of Parasitology, Leiden University Medical Centre, Postbus 9600 RC, The Netherlands
    Mol Biochem Parasitol 137:23-33. 2004
    ..With the recent development of a second, independent selectable marker in P. berghei, PbGFPCON is a useful tool to investigate the effect of further genetic modifications on host-parasite interactions...
  75. ncbi Inducible cAMP early repressor suppresses gastrin-mediated activation of cyclin D1 and c-fos gene expression
    Tonje S Steigedal
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    Am J Physiol Gastrointest Liver Physiol 292:G1062-9. 2007
    ..Our finding that ICER modulates pituitary adenylate cyclase-activating polypeptide-activated gene expression also indicates a regulatory effect of ICER in the responses of neuroendocrine cells to peptides other than gastrin...
  76. pmc Predictive and interpretive simulation of green fluorescent protein expression in reporter bacteria
    J H Leveau
    Department of Plant and Microbial Biology, University of California, 111 Koshland Hall, Berkeley, CA 94720, USA
    J Bacteriol 183:6752-62. 2001
    ....
  77. ncbi Direct regulation of beta3-integrin subunit gene expression by HOXA10 in endometrial cells
    Gaurang S Daftary
    Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut 06520 8063, USA
    Mol Endocrinol 16:571-9. 2002
    ..beta3-Integrin expression was directly up-regulated by HOXA10 through a 41-bp 5'-regulatory element. Sex steroids regulate the expression of endometrial beta3-integrin through a pathway involving HOXA10...
  78. pmc A switch from MafB to MafA expression accompanies differentiation to pancreatic beta-cells
    Wataru Nishimura
    Section of Islet Transplantation and Cell Biology, Joslin Diabetes Center, Boston, MA 02215, USA
    Dev Biol 293:526-39. 2006
    ..Thus, this redundancy in the function and expression of the large-Maf factors may explain the normal islet morphology observed in the MafA knockout mice at birth...
  79. ncbi Selective small molecule inhibitors of glycogen synthase kinase-3 modulate glycogen metabolism and gene transcription
    M P Coghlan
    Department of Vascular Biology, SmithKline Beecham Pharmaceuticals, Essex, UK
    Chem Biol 7:793-803. 2000
    ..This report describes the identification and characterisation of potent and selective small molecule inhibitors of GSK-3...
  80. pmc E-box function in a period gene repressed by light
    Daniela Vallone
    Max Planck Institut fur Entwicklungsbiologie, Spemannstrasse 35 39, D 72076 Tubingen, Germany
    Proc Natl Acad Sci U S A 101:4106-11. 2004
    ..Thus, these results reveal flexibility in the phase and light responsiveness of E-box-directed rhythmic expression, depending on the promoter context...
  81. ncbi Identification of plasminogen activator inhibitor-2 as a gastrin-regulated gene: Role of Rho GTPase and menin
    Andrea Varro
    Physiological Laboratory, University of Liverpool, Liverpool, England
    Gastroenterology 123:271-80. 2002
    ..The gastric hormone gastrin regulates acid secretion, gene expression, and the functional development and cellular composition of the gastric mucosa. Using a gene array, we sought to identify major, novel, gastrin-regulated genes...
  82. pmc The NC1/endostatin domain of Caenorhabditis elegans type XVIII collagen affects cell migration and axon guidance
    B D Ackley
    Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    J Cell Biol 152:1219-32. 2001
    ..These results indicate that the cle-1 NC1/ES domain regulates cell and axon migrations in C. elegans...
  83. ncbi The aryl hydrocarbon receptor displaces p300 from E2F-dependent promoters and represses S phase-specific gene expression
    Jennifer L Marlowe
    Center for Environmental Genetics and the Department of Environmental Health, University of Cincinnati Medical Center, Cincinnati, Ohio 45267 0056, USA
    J Biol Chem 279:29013-22. 2004
    ..The AHR seems to act as an environmental checkpoint that senses exposure to environmental toxicants and responds by signaling cell cycle inhibition...
  84. ncbi The dynamics of homologous chromosome pairing during male Drosophila meiosis
    Julio Vazquez
    Department of Biochemistry and Biophysics, University of California, San Francisco, 94143, USA
    Curr Biol 12:1473-83. 2002
    ..Due to limitations in available cytological techniques, the early stages of homologous chromosome pairing in male Drosophila have not been observed, and the mechanisms involved are poorly understood...
  85. pmc Ferritin as an endogenous MRI reporter for noninvasive imaging of gene expression in C6 glioma tumors
    Batya Cohen
    Department of Biological Regulation, The Weizmann Institute of Science, Rehovot 76100, Israel
    Neoplasia 7:109-17. 2005
    ..Application of this novel MR reporter gene that generates significant contrast in the absence of exogenously administered substrates opens new possibilities for noninvasive molecular imaging of gene expression by MRI...
  86. ncbi One nucleotide in a kappaB site can determine cofactor specificity for NF-kappaB dimers
    Thomas H Leung
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Cell 118:453-64. 2004
    ..This suggests that binding sites may impart a specific configuration to bound transcription factors...
  87. ncbi Mitogen-activated protein kinases, Erk and p38, phosphorylate and regulate Foxo1
    Sachie Asada
    Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba, Ibaraki 305 8577, Japan
    Cell Signal 19:519-27. 2007
    ..These results suggest that Foxo1 is specifically phosphorylated by Erk and p38, and that this phosphorylation regulates the function of Foxo1 as a coactivator for Ets-1...
  88. ncbi DNA repair pathway stimulated by the forkhead transcription factor FOXO3a through the Gadd45 protein
    Hien Tran
    Division of Neuroscience, Children s Hospital and Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
    Science 296:530-4. 2002
    ..These findings indicate that in mammals FOXO3a regulates the resistance of cells to stress by inducing DNA repair and thereby may also affect organismal life-span...
  89. ncbi Imaging antigen-induced PI3K activation in T cells
    Julie Harriague
    Departement de Biologie Cellulaire, Institut Cochin, INSERM U567, CNRS UMR 7627, Universite Rene Descartes, 22 rue Mechain, 75014 Paris, France
    Nat Immunol 3:1090-6. 2002
    ..In T cell-APC conjugates, PIP3 accumulated at the T cell-APC synapse as well as in the rest of the T cell plasma membrane, which indicated unusual regulation of PI3K activity during antigen presentation...
  90. ncbi Continued maturation of thymic emigrants in the periphery
    Tamar E Boursalian
    Department of Immunology, University of Washington, Seattle, Washington 98195 7650, USA
    Nat Immunol 5:418-25. 2004
    ..In addition, the relative contribution of CD4 and CD8 recent thymic emigrants was modulated as they entered the peripheral T cell pool. Thus, T cell maturation and subset contribution are both finalized in the lymphoid periphery...
  91. ncbi Coupled mRNA stabilization and translational silencing of cyclooxygenase-2 by a novel RNA binding protein, CUGBP2
    Debnath Mukhopadhyay
    Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Cell 11:113-26. 2003
    ..These findings identify a novel paradigm for RNA binding proteins in facilitating opposing functions of mRNA stability and translation inhibition and reveal a mechanism for inhibiting COX-2 expression in cancer cells...
  92. pmc Large scale transgenic and cluster deletion analysis of the HoxD complex separate an ancestral regulatory module from evolutionary innovations
    F Spitz
    Department of Zoology and Animal Biology, University of Geneva, Sciences III, 1211 Geneva 4, Switzerland
    Genes Dev 15:2209-14. 2001
    ..Accordingly, deletion of the entire cluster abolished axial expression, whereas recently acquired regulatory controls were preserved...
  93. ncbi Mae mediates MAP kinase phosphorylation of Ets transcription factors in Drosophila
    D A Baker
    Cell Signalling Unit, Institute of Reproductive and Developmental Biology, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
    Nature 411:330-4. 2001
    ..Our study indicates that MAPK signalling specificity may depend on proteins that couple specific substrates to the kinase...
  94. ncbi Cassettes for PCR-mediated construction of green, yellow, and cyan fluorescent protein fusions in Candida albicans
    M Gerami-Nejad
    Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Yeast 18:859-64. 2001
    ..Thus, this technique directs one-step construction of multiple fluorescent protein fusions, facilitating the study of protein co-expression and co-localization in C. albicans cells in vivo...
  95. ncbi Generation of destabilized green fluorescent protein as a transcription reporter
    X Li
    Laboratories, Inc, Palo Alto, California 94303, USA
    J Biol Chem 273:34970-5. 1998
    ..Because of its autofluorescence, destabilized EGFP can be used to directly correlate gene induction with biochemical change, such as NFkappaB translocation to the nucleus...
  96. ncbi Effects of iron on DNA release and biofilm development by Pseudomonas aeruginosa
    Liang Yang
    Centre for BioScience and Technology, BioCentrum DTU, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Microbiology 153:1318-28. 2007
    ....
  97. ncbi A beta-catenin/engrailed chimera selectively suppresses Wnt signaling
    W T Montross
    Department of Biochemistry and Molecular Biology, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    J Cell Sci 113:1759-70. 2000
    ....
  98. pmc Transplantation of bone marrow-derived very small embryonic-like stem cells attenuates left ventricular dysfunction and remodeling after myocardial infarction
    Buddhadeb Dawn
    Institute of Molecular Cardiology, University of Louisville, Louisville, Kentucky 40292, USA
    Stem Cells 26:1646-55. 2008
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  99. pmc A member of the cAMP receptor protein family of transcription regulators in Mycobacterium tuberculosis is required for virulence in mice and controls transcription of the rpfA gene coding for a resuscitation promoting factor
    Lisa Rickman
    Division of Mycobacterial Research, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
    Mol Microbiol 56:1274-86. 2005
    ..In summary, it has been shown that Rv3676 is a direct regulator of rpfA expression, and because rpfA codes for a resuscitation promoting factor this may implicate Rv3676 in reactivation of dormant M. tuberculosis infections...
  100. pmc A secreted luciferase for ex vivo monitoring of in vivo processes
    Thomas Wurdinger
    Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, 149 13th St, Charlestown, Massachusetts 02129, USA
    Nat Methods 5:171-3. 2008
    ....
  101. pmc Th2 cell hyporesponsiveness during chronic murine schistosomiasis is cell intrinsic and linked to GRAIL expression
    Justin J Taylor
    Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104 4539, USA
    J Clin Invest 119:1019-28. 2009
    ..Together, these data indicate that the decline in Th2 cell responsiveness during chronic schistosomiasis is the net result of the upregulation of GRAIL expression in response to repeated Ag stimulation...

Research Grants75

  1. Genomic Enhancers at 8q24 and Prostate Cancer
    Gerhard A Coetzee; Fiscal Year: 2013
    ..Additionally, in vivo enhancer activities will be investigated in mice carrying reporter genes controlled by candidate enhancer elements (subaim 1.2)...
  2. Characterize functions of T. brucei RAP1 and TRF in antigenic variation and telom
    Bibo Li; Fiscal Year: 2013
    ..brucei cells, telomeres form a heterochromatic structure that affects the transcription of reporter genes targeted to subtelomeres...
  3. A XENOPUS LAEVIS RESEARCH RESOURCE FOR IMMUNOBIOLOGY
    Jacques Robert; Fiscal Year: 2013
    ..transgenesis techniques and generating transgenic, which are isogenetic clones, expressing fluorescent reporter genes in lymphoid tissues (e.g...
  4. Derivation of cerebral cortical GABAergic interneurons from human iPS cells
    Lorenz P Studer; Fiscal Year: 2010
    ..labs at i) using modified bacterial artificial chromosomes (BACs) to obtain tissue-specific expression of reporter genes in transgenic mice as well as in mouse and human embryonic stem cells ii) developing highly efficient ..
  5. INDUCIBLE PLURIPOTENT STEM CELLS AND KIDNEY REGENERATION
    JOSEPH VINCENT BONVENTRE; Fiscal Year: 2010
    ..The activation of the reporter genes odd-skipped related 1 (Osr1) and Six2 by small molecules will signify the successful differentiation of mouse ..
  6. Gene-Targeted Pro-Drug Therapy for Colorectal Cancer
    Hiremagalur N Jayaram; Fiscal Year: 2013
    ..tumors (that express FR-, CEA-, and hNMNAT2), we will first individually clone specialized Luciferase (Luc) reporter genes in AAV2 (AAV2-Luc, AAV2-CEA-Luc and AAV2-hNMNAT2-Luc);then we will inject these viruses into mice and assess ..
  7. TRANSCRIPTION FACTORS IN CONTROL OF BRAIN DEVELOPMENT
    Eric E Turner; Fiscal Year: 2010
    ..In the current award period we have identified a sensory-specific enhancer region of the Brn3a gene, and used reporter genes targeted by this enhancer to show marked defects in sensory axon growth in Brn3a knockout mice...
  8. Lentiviral-transduced hematopoictic stem cell transplantation for cystinosis
    Stephanie Cherqui; Fiscal Year: 2013
    ..In Specific aim 1, we propose to optimize the transduction of murine Sca1+ HSC using LV expressing reporter genes to enhance the safety and efficiency of a clinical trial as well as significantly reduce costs...
  9. PAX2 gene targets, binding sites and function
    Patrick D Brophy; Fiscal Year: 2010
    ..binding of Pax2 to genomic loci will be assayed and the ability of Pax2 to transactivate transcription of reporter genes, using upstream regulatory elements from candidate targets, will be determined...
  10. Innovative approach for high-volume production of endogenous reporter cells
    ROBERT E FINNEY; Fiscal Year: 2012
    ..In contrast, in an endogenous reporter, reporter genes are introduced at their normal chromosomal locus where native promoters, regulatory elements, local ..
  11. Computational Design of Small Molecule Drugs Inhibiting the Zinc Finger Transcrip
    David Sherris; Fiscal Year: 2010
    ..Previously, through standard recombinant DNA methods, VEZF1 expression vectors and VEZF1 responsive promoter-reporter genes were generated, the latter as cell-active luciferase reporters that quantitatively establish transcription ..
  12. Detoxification of Electrophilic Chemical Threat Agents by Nucleophilic Scavengers
    Michael C MacLeod; Fiscal Year: 2010
    ..pyknotic/apoptotic nuclei, edema, vesication, infiltration, fibrosis) and induction of mutations in reporter genes carried in the transgenic mutation-reporter mice...
  13. Regulated Transgene Expression in the Retina
    James M Wilson; Fiscal Year: 2010
    ..inducible transgene expression called ARGENT will be developed in retina of mice and monkeys using convenient reporter genes. Studies will be performed with vectors encoding the humanized VEGF antibody called Avastin in mouse models of ..
  14. Identification of the transcriptional regulators of chondrocyte hypertrophy
    ANDREW BRUCE LASSAR; Fiscal Year: 2013
    ..FoxA2 and interacts with a sequence that is necessary to drive chondrocyte-specific expression of linked reporter genes. Recent work in the Lassar lab has established: (1) that FoxA factors are specifically expressed in ..
  15. c-Myc transcription in intestinal growth, differentiation, and carcinogenesis
    Gregory S Yochum; Fiscal Year: 2012
    ..We will test whether this binding is functional by measuring the activities of reporter genes containing various combinations of mutations in the two 5'TCF binding sites and the 3'enhancer...
  16. CONTROL OF BACTERIAL TOXINS BY VIRUSES AND PLASMIDS
    Randall K Holmes; Fiscal Year: 2013
    ..glutamicum systems in which recombinant IdeR controls expression of reporter genes, and then in M. tuberculosis. Because ideR is essential in M...
  17. Protein methyltransferases as transcriptional coregulators
    Michael R Stallcup; Fiscal Year: 2013
    ..Analyses will be performed on both transiently transfected reporter genes and endogenous target genes of G9a and GLP...
  18. Imaging of gene delivery in the central nervous system
    Assaf A Gilad; Fiscal Year: 2010
    ..of gene delivery to the central nervous system (CNS) based on artificial Magnetic Resonance Imaging (MRI) reporter genes such as the Lysine Rich Protein (LRP) reporter gene, which we have previously developed for tracking cells...
  19. Osteoclasts from pluripotent stem cells as vectors to modify bone marrow environm
    Hector L Aguila; Fiscal Year: 2010
    ..Aim 2. Generation of human ES cell lines expressing reporter genes under the control of myeloid specific promoters and validation of their expression in vitro...
  20. Yeast Based Assays for Chemical Screens Against SARS-CoV Targets
    DANIEL A contact ENGEL; Fiscal Year: 2010
    ..antiviral activity of hits from the screen against recombinant SARS-CoV and other human coronaviruses encoding reporter genes. The ultimate goal of this proposal is to develop a rational, high throughput yeast-based screen that ..
  21. Development of a human Oct-4 gene-targeted reporter cell line
    GREGORY LENO; Fiscal Year: 2010
    ..current cell-based high-throughput drug screening technology utilizes randomly integrated (non-targeted) reporter genes that may lack genetic and/or epigenetic regulatory elements that govern endogenous gene expression...
  22. Impact of maternal hyperleptinemia on health: CNS mechanisms
    Rebecca Leshan; Fiscal Year: 2013
    ..We will additionally expressing reporter genes in specific populations of cells within the mouse brain, enabling the visualization of neuronal projections ..
  23. Novel Screen to Identify Arenavirus Inhibitors
    Juan Lama; Fiscal Year: 2013
    ..overcome this problem, we have generated recombinant lymphocytic choriomeningitis viruses (rLCMV) expressing reporter genes of interest...
  24. Development of AAV vectors for the phenotypic correction of sickle cell disease
    Angela Rivers; Fiscal Year: 2013
    ..The transduction efficiency of the novel AAV capsids will be tested using reporter genes expressing enhanced green fluorescence protein (EGFP) and luciferase, and the optimum vector selected to ..
  25. Novel molecular imaging approaches to monitor gene and cell-based therapies
    Shahriar Yaghoubi; Fiscal Year: 2013
    ..Instead of commonly used, highly immunogenic viral proteins, we will generate novel PET reporter genes based on fully human proteins, to overcome this challenge to clinical implementation...
  26. Astroglial Cells in Perinatal Brain Injury
    Flora M Vaccarino; Fiscal Year: 2012
    ..This will be accomplished by genetically marking astroglial cells with reporter genes using a transgenic mouse carrying a drug-inducible Cre recombinase gene expressed under the GFAP promoter...
  27. Analysis of neurodevelopment using mouse mutagenesis
    David R Beier; Fiscal Year: 2012
    ..We will also screen for mutations that perturb patterning of reporter genes that mark specific anatomical structures, A third strategy is to screen for mutations that have a genetic ..
  28. Development of GM-CSF reporter and reporter/fate-reporter mice
    Bogoljub Ciric; Fiscal Year: 2013
    ..The principal conceptual innovation in the transgenic mice that we want to generate is the presence of two reporter genes, one that reports ongoing GM-CSF expression, the other reporting that GM-CSF has been expressed...
  29. Kidney-targeted gene delivery for cystinosis
    Stephanie Cherqui; Fiscal Year: 2012
    ..The optimal dose will be also determined. scAAV expressing the green fluorescent protein (GFP) and luciferase reporter genes will be used in this aim and expression will be visualized and quantified using confocal microscopy, flow ..
  30. Fate-mapping temporally marked precursor populations to hepatocellular carcinoma
    Hyam L Leffert; Fiscal Year: 2013
    ..carcinogen feeding, by precisely timed, and transient Ad-Afp-Cre infection, to activate heritable ROSA {ZSG1} reporter genes solely in AFP+ oval cells from this narrow time window, and their subsequent descendants...
  31. NEURONAL CONTROL OF CGRP GENE EXPRESSION
    ANDREW RUSSO; Fiscal Year: 2009
    ..We have introduced reporter genes into primary cultures of rat trigeminal ganglia neurons and have begun studies monitoring promoter activity in ..
  32. Circadian Clock Genes in Bipolar Disorder
    MICHAEL JOSEPH MCCARTHY; Fiscal Year: 2013
    ..We have employed specialized reporter genes to facilitate these studies...
  33. Var gene regulation and antigenic variation in malaria
    Kirk W Deitsch; Fiscal Year: 2013
    ..The experimental design relies on creating transgenic parasite lines using reporter genes and drug selectable markers to rapidly determine the role of these regulatory elements in controlling var gene ..
  34. Development of Paratransgenic ticks for Disease Control
    ULRIKE GERTRUD MUNDERLOH; Fiscal Year: 2013
    ..2) transgenic rickettsiae expressing reporter genes can be used to create a tick paratransgenesis model that will enable us to examine and identify the biological ..
  35. Imaging Cytolytic T Cells in Cancer Patients Using PET Reporter Genes/Reporter Pr
    Sanjiv S Gambhir; Fiscal Year: 2012
    ..Positron emission tomography (PET) reporter genes (PRG)/probes (PRP) can be used for long-term monitoring of ex-vivo genetically engineered therapeutic cells in ..
  36. Risk for in vivo mutagenesis of the P53 gene by nucleoside analog antiviral drug
    Vernon E Walker; Fiscal Year: 2011
    ..AZT) are accompanied by host cell AZT-DNA incorporation, cytogenetic effects, and mutagenic responses in reporter genes like glyophorin A and HPRT of HIV-infected mothers and their newborns...
  37. Molecular Imaging of Cardiac Stem Cell Therapy
    Joseph C Wu; Fiscal Year: 2013
    ..In particular, we will develop and validate various reporter genes that will enable us to image fate of cardiac stem cells (CSC) in vivo...
  38. Colony Stimulating Factor 1: Role in Photosensitive Cutaneous and Systemic Lupus
    Vicki Rubin Kelley; Fiscal Year: 2010
    ..MRL-Faslpr mice expressing distinct levels of CSF-1 (high, intermediate, none), individual CSF-1 isoforms, and reporter genes for identifying cells expressing CSF-1 and CSF-1 receptors (R) in tissues...
  39. Myogenic stem cells in extraocular muscles
    Zipora Yablonka-Reuveni; Fiscal Year: 2013
    ..Cells from wildtype and dystrophin-null mice that carry various reporter genes (for tracing the different cell types and their progeny) will be investigated in vivo and in culture...
  40. High throughput screens for modulators of inflammatory cytakine gene expression
    Stephen T Smale; Fiscal Year: 2010
    ..Specifically, we are generating macrophage cell lines from mice in which fluorescent protein reporter genes are regulated by cytokine gene control regions in their native chromatin environment...
  41. 3'alpha enhancer regulation by AhR and NF-kappaB/Rel proteins
    COURTNEY ELIZABETH WILLIAMS SULENTIC; Fiscal Year: 2011
    ..Determine if the polymorphic human hs1,2 enhancer is sensitive to TCDD- induced inhibition with luciferase reporter genes regulated by the human hs1,2 enhancer...
  42. MULTIPLEX CIS-REGULATORY ANALYSIS IN MAMMALIAN CELLS
    Joseph C Corbo; Fiscal Year: 2012
    ..to harness the power of Next Generation Sequencing (NGS) to create a system for the multiplexed analysis of reporter genes in mammalian cells...
  43. Molecular determinants of hepatitis C virus assembly
    Brett D Lindenbach; Fiscal Year: 2013
    ..a highly efficient system for growing HCV in cell culture;2) engineered infectious virus variants with useful reporter genes, affinity tags, and fluorescent markers;3) conducted a mutagenesis study on the viral NS2 gene, revealing ..
  44. Transgenic Tools for Regulated Gene Expression in Zebrafish
    Marnie E Halpern; Fiscal Year: 2013
    ..We aim to establish a collection QF driver lines that activate reporter genes in unique, tissue-specific patterns...
  45. ENHANCER-MEDIATED MECHANISMS OF PATTERN FORMATION
    Stephen J Small; Fiscal Year: 2013
    ..In Aim 3, we will use reporter genes, bio-informatics studies, and a genome-wide interaction assay to probe the mechanisms that control promoter ..
  46. Ultrasound mediated targeted therapy for kidney disease
    Joshua J Rychak; Fiscal Year: 2010
    ..This method has shown efficacy for delivering reporter genes to perivascular tissue in mice and rats, although targeted accumulation of the therapeutic microbubble at the ..
  47. Developing tools for genetic manipulation of hookworms
    John M Hawdon; Fiscal Year: 2013
    ..In Aim 1, particle bombardment will be used to introduce reporter genes into embryos and larval stages, and transformants will be assayed for survival, growth and reporter gene ..
  48. Stem cell niche and Wnt in liver injury and regeneration.
    BRUCE MAO ZHENG WANG; Fiscal Year: 2013
    ..Through the use of reporter genes linked to this enhancer, CAIRE function has been examined in various tissues...
  49. Analysis of ubiquitination enzymes in C. elegans
    Lynn Boyd; Fiscal Year: 2009
    ..Specific Aim #2 proposes to study the tissue specific expression of different Ubcs by means of reporter genes linked to Ubc promoters...
  50. Caliper Life Sciences IVIS Lumina II imaging system
    Brad T Cookson; Fiscal Year: 2012
    ..PUBLIC HEALTH RELEVANCE: The IVIS(r) Lumina II uses an ultrasensitive camera to detect light from reporter genes and will provide anatomically localized information about cellular activities, gene expression, inflammation ..
  51. General method for targeting genes to specific neuronal subtypes in mammals
    Anthony M Zador; Fiscal Year: 2013
    ..We propose to develop a novel strategy for the expression of reporter genes in specific neuronal subtypes...
  52. THE PATHWAY THAT SETS THE CYANOBACTERIAL CIRCADIAN CLOCK
    Susan S Golden; Fiscal Year: 2013
    ..to genetic manipulation and its circadian rhythms of gene expression can be visualized through luciferase reporter genes. Clock mechanism across all groups of organisms is operationally divided into the oscillator, the input ..
  53. MONITORING STEM CELL GRAFTS USING A NOVEL MRI REPORTER
    Anthony Wing Sang Chan; Fiscal Year: 2012
    ..Initial studies with ferritin have generated promising results that suggest the potential of MRI reporter genes 2...
  54. Activatable MR Imaging Probes
    PETER D CARAVAN; Fiscal Year: 2013
    ..MR probes responsive to Ca2+ flux, Zn2+ flux, reporter genes, enzymatic activity, pO2, and pH have been reported...
  55. Gene Delivery in Retinal Diseases
    John M Nickerson; Fiscal Year: 2013
    ..In the past grant period, we demonstrated high in vivo expression of reporter genes in mouse RPE following ocular electroporation with plasmid DNA...
  56. A novel and improved mouse system for studying macrophage specific gene deletion
    Christian Stehlik; Fiscal Year: 2012
    ..Transgenic mice will be extensively characterized with reporter genes and endogenous floxed genes and compared to the currently available Cre-expressing mice...
  57. Development of Molecular Imaging Technology Visualizing Viral Reservoirs in Vivo
    Won Bin Young; Fiscal Year: 2013
    ..a replication-competent lentivirus, such as simian or feline immunodeficiency virus (SIV or FIV), with reporter genes in its genome, thus, each infected cell will express reporters delivered by the lentiviral infection and ..
  58. MASSIVELY PARALLEL CIS-REGULATORY ELEMENT ANALYSIS IN MAMMALIAN CELLS
    Joseph C Corbo; Fiscal Year: 2013
    ..Our strategy is to fuse libraries of CREs to barcoded reporter genes, transfect these libraries into cells and quantify their output by NGS...
  59. Bioluminescent reporter phage for the diagnostic detection of shigellosis
    David A Schofield; Fiscal Year: 2013
    ..Aim 2 will integrate the bacterial luxAB reporter genes into non-essential sites of th prioritized Shigella phage genomes to create luxAB-tagged reporter phages...
  60. Master Regulators of Transcription in the C. crescentus cell cycle
    Celeste Peterson; Fiscal Year: 2010
    ..The library will also be screened for defects in the stationary phase response by reduced expression of reporter genes. The second aim is to characterize a mutant, iscR, found in the pilot screen for Aim 1...
  61. Non-invasive imaging of progenitor cell fate in the ischemic myocardium
    Martin Rodriguez-Porcel; Fiscal Year: 2013
    ..Aim 1 will test the hypothesis that the oxidant status of PCs can be monitored using reporter genes. Aim 2 will test the hypothesis that a) the oxidant status of transplanted PCs can be monitored noninvasively ..
  62. TRANSCRIPTIONAL CONTROL OF MAMMALIAN BRAIN DEVELOPMENT
    Eseng Lai; Fiscal Year: 2001
    ..hyperphosphorylation of retinoblastoma, block of induction of p15 mRNA levels, and block of activation of reporter genes driven by a TGF-b- responsive promoter...
  63. Transcriptional Control of the Helicobacter pylori RpoN Regulon
    TIMOTHY RANDALL HOOVER; Fiscal Year: 2010
    ..a constitutively active form of FlgR bypasses export apparatus mutations to allow expression of RpoN-dependent reporter genes. In the second specific aim H...
  64. MECHANISMS IN THE REGULATION OF SP-A GENE EXPRESSION
    CAROLE MENDELSON; Fiscal Year: 2007
    ..In studies using transgenic mice[unreadable] carrying SP-A:human growth hormone (hGH) reporter genes, we have found[unreadable] that as little as 400 bp of SP-A 5'-flanking sequence mediates lung-[..
  65. PROMYELOCYTIC ZINC FINGER GENE
    Jennifer Morris; Fiscal Year: 1999
    ..PLZF expression vectors will be cotransfected with chloramphenicol acetyl transferase (CAT) reporter genes containing PLZF binding sites into mouse fibroblasts and myeloid progenitor cell lines...
  66. Northeastern Ohio Animal Imaging Resource Center- NOAIRC
    Jeffrey Duerk; Fiscal Year: 2006
    ..our ongoing biomolecular and nanoscale engineering for targeted therapeutics initiative, ongoing research in reporter genes, and a P20 In-vivo and Molecular Imaging Center planning grant to add strategic strength in molecular imaging...
  67. COMPLEMENTARY DNA PROBES TO A STEROID RECEPTOR GENE
    J Pike; Fiscal Year: 1992
    ..Transcriptional evaluation will be made by employing natural and cloned target gene promoters as reporter genes for transcriptional activity (CaBP, osteocalcin, MMTV)...
  68. SIMIAN RETROVIRUSES--NOVEL GENE DELIVERY VECTORS
    CURTIS MACHIDA; Fiscal Year: 1999
    ..primate and human cell lines, and then utilize this vector as a gene delivery vehicle for the expression of reporter genes in nonhuman primates...
  69. TRYPANOSOMA CRUZI: PATHOGENESIS OF INFECTION
    Herbert Tanowitz; Fiscal Year: 2000
    ..We will co-transfect dominant negative genes of the kinase cascades with AP-1 and ET-1 reporter genes to delineate the activation of pathways...
  70. Transposon-mediated Gene Therapy for Fanconi Anemia
    PERRY HACKETT; Fiscal Year: 2004
    ..DGI). In Specific Aim 1, we will first test the combination of these two technologies by using reporter genes to ascertain long-term gene transfer and expression in cultured hematopoietic cells...
  71. RAC 1 SIGNALING PATHWAY IN AIRWAY SMOOTH MUSCLE
    MARC HERSHENSON; Fiscal Year: 2002
    ..with plasmids encoding the human cyclin D1 and cyclin D1 and cyclin A promoters subcloned into luciferase reporter genes. 2: Characterize the downstream signaling intermediates and promoter elements required for Rac1-induced G1 ..
  72. NOVEL NONVIRAL VECTORS FOR GENE TRANSFER
    Thomas Scanlin; Fiscal Year: 2000
    ..gene transfer vectors, and have been shown to be effective and efficient for the transfer of reporter genes and the cystic fibrosis transmembrane conductance regulator (CFTR) gene into airway epithelial cells from ..
  73. Discovery of novel fluorescent reporter genes
    Ming Zhao; Fiscal Year: 2004
    ..Reporters with spectral properties that can be used for whole-body imaging of tumors in the lung and their metastases will be candidates for further development for numerous applications of multi-color imaging. ..
  74. Image Based Evaluation of Tumor Targeting and Efficacy of Gene Therapy
    Vikas Kundra; Fiscal Year: 2010
    ..b>Reporter genes can both locate and quantify expression...
  75. TRANSGENIC REPORTERS OF ENVIRONMENTAL GENOTOXICANTS
    Peter Stambrook; Fiscal Year: 2000
    ..The ability to produce transgenic mice with reporter genes that can monitor mutagenic activity in tissues and organs of tester animals represents a significant ..