cdc genes

Summary

Summary: Genes that code for proteins that regulate the CELL DIVISION CYCLE. These genes form a regulatory network that culminates in the onset of MITOSIS by activating the p34cdc2 protein (PROTEIN P34CDC2).

Top Publications

  1. ncbi The spindle-assembly checkpoint in space and time
    Andrea Musacchio
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy
    Nat Rev Mol Cell Biol 8:379-93. 2007
  2. pmc Roles of Hof1p, Bni1p, Bnr1p, and myo1p in cytokinesis in Saccharomyces cerevisiae
    E A Vallen
    Department of Biology, Swarthmore College, Swarthmore, Pennsylvania 19081, USA
    Mol Biol Cell 11:593-611. 2000
  3. pmc Aurora B couples chromosome alignment with anaphase by targeting BubR1, Mad2, and Cenp-E to kinetochores
    Claire Ditchfield
    School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Rd, Manchester M13 9PT, UK
    J Cell Biol 161:267-80. 2003
  4. ncbi A central role for DNA replication forks in checkpoint activation and response
    José Antonio Tercero
    Cancer Research UK, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, United Kingdom
    Mol Cell 11:1323-36. 2003
  5. ncbi Checking on DNA damage in S phase
    Jiri Bartek
    Danish Cancer Society, Institute of Cancer Biology, Strandboulevarden 49, DK 2100 Copenhagen, Denmark
    Nat Rev Mol Cell Biol 5:792-804. 2004
  6. pmc The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore-microtubule attachment and in maintaining the spindle assembly checkpoint
    Silke Hauf
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    J Cell Biol 161:281-94. 2003
  7. ncbi Forkhead transcription factors contribute to execution of the mitotic programme in mammals
    B Alvarez
    Department of Immunology and Oncology, , , , Cantoblanco, Madrid E-28049, Spain
    Nature 413:744-7. 2001
  8. pmc Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2
    V Sudakin
    Institute for Cancer Research, The Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    J Cell Biol 154:925-36. 2001
  9. ncbi Cell-cycle checkpoints that ensure coordination between nuclear and cytoplasmic events in Saccharomyces cerevisiae
    D J Lew
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, 27710, USA
    Curr Opin Genet Dev 10:47-53. 2000
  10. ncbi ATM and related protein kinases: safeguarding genome integrity
    Yosef Shiloh
    The David and Inez Myers Laboratory for Genetic Research, Department of Human Genetics and Molecular Medicine, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Nat Rev Cancer 3:155-68. 2003

Detail Information

Publications191 found, 100 shown here

  1. ncbi The spindle-assembly checkpoint in space and time
    Andrea Musacchio
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy
    Nat Rev Mol Cell Biol 8:379-93. 2007
    ..These studies are finally starting to reveal the mechanisms of checkpoint activation and silencing during mitotic progression...
  2. pmc Roles of Hof1p, Bni1p, Bnr1p, and myo1p in cytokinesis in Saccharomyces cerevisiae
    E A Vallen
    Department of Biology, Swarthmore College, Swarthmore, Pennsylvania 19081, USA
    Mol Biol Cell 11:593-611. 2000
    ..In bni1Delta cells that lack the actomyosin contraction, septum formation is often slow and asymmetric, suggesting that actomyosin contraction may provide directionality for efficient septum formation...
  3. pmc Aurora B couples chromosome alignment with anaphase by targeting BubR1, Mad2, and Cenp-E to kinetochores
    Claire Ditchfield
    School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Rd, Manchester M13 9PT, UK
    J Cell Biol 161:267-80. 2003
    ..Together, these results suggest that by targeting checkpoint proteins to kinetochores, Aurora B couples chromosome alignment with anaphase onset...
  4. ncbi A central role for DNA replication forks in checkpoint activation and response
    José Antonio Tercero
    Cancer Research UK, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, United Kingdom
    Mol Cell 11:1323-36. 2003
    ..Thus, DNA replication forks are both activators and primary effectors of the checkpoint pathway in S phase...
  5. ncbi Checking on DNA damage in S phase
    Jiri Bartek
    Danish Cancer Society, Institute of Cancer Biology, Strandboulevarden 49, DK 2100 Copenhagen, Denmark
    Nat Rev Mol Cell Biol 5:792-804. 2004
    ....
  6. pmc The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore-microtubule attachment and in maintaining the spindle assembly checkpoint
    Silke Hauf
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    J Cell Biol 161:281-94. 2003
    ..Together, our data suggest that Aurora B is required to generate unattached kinetochores on monooriented chromosomes, which in turn could promote bipolar attachment as well as maintain checkpoint signaling...
  7. ncbi Forkhead transcription factors contribute to execution of the mitotic programme in mammals
    B Alvarez
    Department of Immunology and Oncology, , , , Cantoblanco, Madrid E-28049, Spain
    Nature 413:744-7. 2001
    ....
  8. pmc Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2
    V Sudakin
    Institute for Cancer Research, The Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    J Cell Biol 154:925-36. 2001
    ..We propose that the preformed interphase pool of MCC allows for rapid inhibition of APC/C when cells enter mitosis. Unattached kinetochores then target the APC/C for sustained inhibition by the MCC...
  9. ncbi Cell-cycle checkpoints that ensure coordination between nuclear and cytoplasmic events in Saccharomyces cerevisiae
    D J Lew
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, 27710, USA
    Curr Opin Genet Dev 10:47-53. 2000
    ..Thus, specific cell-cycle regulators are well-placed to monitor whether a cell has formed a bud and whether a daughter nucleus has been delivered accurately to the bud following mitosis...
  10. ncbi ATM and related protein kinases: safeguarding genome integrity
    Yosef Shiloh
    The David and Inez Myers Laboratory for Genetic Research, Department of Human Genetics and Molecular Medicine, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Nat Rev Cancer 3:155-68. 2003
    ..Understanding ATM's mode of action provides new insights into the association between defective responses to DNA damage and cancer, and brings us closer to resolving the issue of cancer predisposition in some A-T carriers...
  11. ncbi Chfr defines a mitotic stress checkpoint that delays entry into metaphase
    D M Scolnick
    The Wistar Institute, Philadelphia, Pennsylvania 19104 4268, USA
    Nature 406:430-5. 2000
    ..Ectopic expression of wild-type chfr restored the cell cycle delay and increased the ability of the cells to survive mitotic stress. Thus, chfr defines a checkpoint that delays entry into metaphase in response to mitotic stress...
  12. pmc RAD53, DUN1 and PDS1 define two parallel G2/M checkpoint pathways in budding yeast
    R Gardner
    Department of Molecular and Cellular Biology, The University of Arizona, PO Box 21016, Tucson, AZ 85721 0106, USA
    EMBO J 18:3173-85. 1999
    ..Instead, we think it likely that both pathways contribute to establishing and maintaining arrest...
  13. ncbi A p53-dependent checkpoint pathway prevents rereplication
    Cyrus Vaziri
    Cancer Center, Department of Medicine, Boston University School of Medicine, 80 East Concord Street, Massachusetts 02118, USA
    Mol Cell 11:997-1008. 2003
    ..p53 inhibits rereplication through the induction of the cdk2 inhibitor p21. Therefore, a p53-dependent checkpoint pathway is activated to suppress rereplication and promote genetic stability...
  14. ncbi Does aneuploidy cause cancer?
    Beth A A Weaver
    Ludwig Institute for Cancer Research, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0670, USA
    Curr Opin Cell Biol 18:658-67. 2006
    ..Cumulatively, the current evidence suggests that aneuploidy promotes tumorigenesis, at least at low frequency, but a definitive test has not yet been reported...
  15. ncbi DNA damage checkpoints: from initiation to recovery or adaptation
    Jiri Bartek
    Department of Cell Cycle and Cancer, and Centre for Genotoxic Stress Research, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, DK 2100 Copenhagen, Denmark
    Curr Opin Cell Biol 19:238-45. 2007
    ..Such studies highlight the dynamic nature of these processes and help us to better understand the molecular basis, spatiotemporal orchestration and biological significance of the DNA damage response in normal and cancerous cells...
  16. ncbi The impact of a negligent G2/M checkpoint on genomic instability and cancer induction
    Markus Lobrich
    Darmstadt University of Technology, Radiation Biology and DNA Repair, 64287 Darmstadt, Germany
    Nat Rev Cancer 7:861-9. 2007
    ..Here, we consider the impact of a negligent G2/M checkpoint on genomic stability and cancer risk...
  17. ncbi Cytokinetic actomyosin ring formation and septation in fission yeast are dependent on the full recruitment of the polo-like kinase Plo1 to the spindle pole body and a functional spindle assembly checkpoint
    Daniel P Mulvihill
    Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
    J Cell Sci 115:3575-86. 2002
    ..We conclude that CAR formation is subject to regulation by the spindle assembly checkpoint via the loading of Plo1 onto the SPBs and the consequent activation of the SIN...
  18. ncbi Choreography of the DNA damage response: spatiotemporal relationships among checkpoint and repair proteins
    Michael Lisby
    Department of Genetics and Development, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA
    Cell 118:699-713. 2004
    ....
  19. ncbi MAD2 haplo-insufficiency causes premature anaphase and chromosome instability in mammalian cells
    L S Michel
    Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 409:355-9. 2001
    ..Furthermore, Mad2+/- mice develop lung tumours at high rates after long latencies, implicating defects in the mitotic checkpoint in tumorigenesis...
  20. ncbi Does the ribosome translate cancer?
    Davide Ruggero
    Molecular Biology Program, Department of Pathology, Memorial Sloan Kettering Cancer Center, Sloan Kettering Institute, 1275 York Avenue, New York, New York 10021, USA
    Nat Rev Cancer 3:179-92. 2003
    ..Although many studies have correlated deregulation of protein biosynthesis with cancer, it remains to be established whether this translates directly into an increase in cancer susceptibility, and under what circumstances...
  21. ncbi The human mitotic checkpoint protein BubR1 regulates chromosome-spindle attachments
    Michael A Lampson
    Laboratory of Chemistry and Cell Biology, Rockefeller University, New York, NY 10021, USA
    Nat Cell Biol 7:93-8. 2005
    ..We propose that BubR1 links regulation of chromosome-spindle attachment to mitotic checkpoint signalling...
  22. pmc The severe slow growth of Deltasrs2 Deltarqh1 in Schizosaccharomyces pombe is suppressed by loss of recombination and checkpoint genes
    Mohamed Maftahi
    Department of Environmental Health Sciences, Columbia University, Mailman School of Public Health and College of Physicians and Surgeons, Kolb Building, Room 114, 722 West 168th Street, New York, NY 10032, USA
    Nucleic Acids Res 30:4781-92. 2002
    ..This lethality is suppressed in a Deltarhp51 background. Together, these results demonstrate a clear genetic interaction between rqh1+, srs2+ and the genes of the homologous recombination pathway...
  23. pmc Cycles of chromosome instability are associated with a fragile site and are increased by defects in DNA replication and checkpoint controls in yeast
    Anthony Admire
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA
    Genes Dev 20:159-73. 2006
    ..Cycles of instability of this normal yeast chromosome may be relevant to chromosome instability of mammalian fragile sites and of chromosomes in cancer cells...
  24. pmc Mathematical model of the morphogenesis checkpoint in budding yeast
    Andrea Ciliberto
    Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    J Cell Biol 163:1243-54. 2003
    ..The model supports the idea that the morphogenesis checkpoint, like other checkpoints, raises the cell size threshold for progression from one phase of the cell cycle to the next...
  25. pmc Rae1 is an essential mitotic checkpoint regulator that cooperates with Bub3 to prevent chromosome missegregation
    J Ramesh Babu
    Department of Pediatric and Adolescent Medicine, Mayo Clinic, Rochester, MN 55905, USA
    J Cell Biol 160:341-53. 2003
    ..Thus, our data demonstrate a novel function for Rae1 and characterize Rae1 and Bub3 as related proteins with essential, overlapping, and cooperating roles in the mitotic checkpoint...
  26. pmc Saccharomyces cerevisiae Rrm3p DNA helicase promotes genome integrity by preventing replication fork stalling: viability of rrm3 cells requires the intra-S-phase checkpoint and fork restart activities
    Jorge Z Torres
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
    Mol Cell Biol 24:3198-212. 2004
    ..The rrm3 system provides a unique opportunity to learn the fate of forks whose progress is impaired by natural impediments rather than by exogenous DNA damage...
  27. pmc Protein phosphatase 1 regulates exit from the spindle checkpoint in budding yeast
    Benjamin A Pinsky
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, PO Box 19024, Seattle, WA 98109, USA
    Curr Biol 19:1182-7. 2009
    ..We therefore propose that PP1 activity silences the checkpoint by reversing key phosphorylation events...
  28. pmc Cell cycle progression after cleavage failure: mammalian somatic cells do not possess a "tetraploidy checkpoint"
    Yumi Uetake
    Department of Cell Biology, University of Massachusetts Medical School, Biotech 4, 3rd Floor, 377 Plantation St, Worcester, MA 01605, USA
    J Cell Biol 165:609-15. 2004
    ..These observations provide a functional demonstration that the tetraploidy checkpoint does not exist in normal mammalian somatic cells...
  29. pmc Lesions in many different spindle components activate the spindle checkpoint in the budding yeast Saccharomyces cerevisiae
    K G Hardwick
    Department of Physiology, University of California, San Francisco, California 94143 0444, USA
    Genetics 152:509-18. 1999
    ..In contrast, the cell cycle arrest caused by mutations that induce DNA damage (cdc13), inactivate the cyclin proteolysis machinery (cdc16 and cdc23), or arrest cells in anaphase (cdc15) is independent of the spindle checkpoint...
  30. pmc Mad2 and BubR1 function in a single checkpoint pathway that responds to a loss of tension
    Katie B Shannon
    SPIRE fellow, Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Mol Biol Cell 13:3706-19. 2002
    ..We conclude that reduced tension at kinetochores with a full complement of kinetochore microtubules induces a checkpoint dependent metaphase delay associated with elevated amounts of kinetochore 3F3/2, Bub1, and BubR1 labeling...
  31. ncbi Human CENP-I specifies localization of CENP-F, MAD1 and MAD2 to kinetochores and is essential for mitosis
    Song Tao Liu
    Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA
    Nat Cell Biol 5:341-5. 2003
    ..The collective output from many unattached kinetochores is required to reach a threshold signal of 'wait for anaphase' to sustain a prolonged mitotic arrest...
  32. ncbi The Mre11 complex: at the crossroads of dna repair and checkpoint signalling
    Damien D'Amours
    Wellcome Trust and Cancer Research, UK Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Nat Rev Mol Cell Biol 3:317-27. 2002
    ..The molecular defect that underlies these phenotypes has long been thought to be related to a DNA repair deficiency. However, recent studies have uncovered functions for the Mre11 complex in checkpoint signalling and DNA replication...
  33. ncbi Comparison of computational methods for the identification of cell cycle-regulated genes
    Ulrik de Lichtenberg
    Center for Biological Sequence Analysis, Technical University of Denmark DK 2800 Lyngby, Denmark
    Bioinformatics 21:1164-71. 2005
    ..We present a simple permutation-based method that performs better than most existing methods...
  34. ncbi Dynamic interaction of DNA damage checkpoint protein Rad53 with chromatin assembly factor Asf1
    A Emili
    Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Mol Cell 7:13-20. 2001
    ..Biochemical and molecular genetic studies suggest that Asf1 is an important target of the Rad53-dependent DNA damage response and that Rad53 may directly regulate chromatin assembly during DNA replication and repair...
  35. ncbi G1 tetraploidy checkpoint and the suppression of tumorigenesis
    Robert L Margolis
    Institut de Biologie Structurale J P Ebel CEA CNRS, 38027 Grenoble Cedex 1, France
    J Cell Biochem 88:673-83. 2003
    ..Further, we discuss the possibility that this mechanism may similarly impose G1 arrest in cells that become aneuploid through errors in mitosis...
  36. ncbi A human homologue of the Schizosaccharomyces pombe rad1+ checkpoint gene encodes an exonuclease
    A E Parker
    Department of Experimental Molecular Biology, Janssen Research Foundation, Turnhoutseweg 30, B 2340 Beerse, Belgium
    J Biol Chem 273:18332-9. 1998
    ..The biological functions of the two proteins remain to be determined...
  37. pmc Aneuploid colon cancer cells have a robust spindle checkpoint
    A Tighe
    School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Manchester M13 9PT, UK
    EMBO Rep 2:609-14. 2001
    ..Significantly, we show here that expression of an APC mutant in MIN cells reduces the mitotic index following spindle damage to a level observed in CIN cells, suggesting that APC dysfunction may contribute to CIN...
  38. pmc BubR1 is essential for kinetochore localization of other spindle checkpoint proteins and its phosphorylation requires Mad1
    Rey Huei Chen
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    J Cell Biol 158:487-96. 2002
    ..This paper demonstrates that BubR1 plays an important role in kinetochore association of other spindle checkpoint proteins and that Mad1 facilitates BubR1 hyperphosphorylation at kinetochores...
  39. ncbi Structure, function, and regulation of budding yeast kinetochores
    Andrew D McAinsh
    Department of Biology, and Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Annu Rev Cell Dev Biol 19:519-39. 2003
    ..location of centromeres? What are the protein components of kinetochores, and how do they assemble a MT attachment site? How do MT attachments generate force? How do cells sense the state of attachment via the spindle assembly checkpoint?..
  40. ncbi The mitotic checkpoint: a signaling pathway that allows a single unattached kinetochore to inhibit mitotic exit
    Gordon K Chan
    Cross Cancer Institute, University of Alberta, Edmonton, Alberta, Canada T6G 1Z2
    Prog Cell Cycle Res 5:431-9. 2003
    ..A single unattached kinetochore is proposed to amplify the "wait anaphase" signal through a kinase cascade involving checkpoint kinases such as hBubR1, hBub1 and Mps1...
  41. ncbi The spindle assembly and spindle position checkpoints
    Daniel J Lew
    Department of Pharmacology and Cancer Biology, Box 3813, Duke University Medical Center, Durham, North Carolina 27710, USA
    Annu Rev Genet 37:251-82. 2003
    ..We review the historical origins of these checkpoints and recent progress in understanding their surveillance pathways. We also highlight fascinating but as yet unresolved questions, and examine crosstalk between the checkpoints...
  42. ncbi Mutational inactivation of mitotic checkpoint genes, hsMAD2 and hBUB1, is rare in sporadic digestive tract cancers
    Y Imai
    Department of Gastroenterology, Faculty of Medicine, University of Tokyo
    Jpn J Cancer Res 90:837-40. 1999
    ..These results indicate that mutations of the hsMAD2 and hBUB1 genes are very rare and presumably play a very restricted role in tumor development of sporadic cancers...
  43. ncbi Developmental regulation of a cyclin-dependent kinase inhibitor controls postembryonic cell cycle progression in Caenorhabditis elegans
    Y Hong
    Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
    Development 125:3585-97. 1998
    ..In vulva precursor cells (VPCs), a pathway of heterochronic genes acts via cki-1 to maintain VPCs in G1 during the L2 stage...
  44. pmc Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores
    Emma M J King
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
    Genes Dev 21:1163-8. 2007
    ..Our data provide strong evidence for a distinct checkpoint pathway responding to lack of sister kinetochore tension, in which Ipl1p-dependent phosphorylation of Mad3p is a key step...
  45. pmc Deletion of mouse rad9 causes abnormal cellular responses to DNA damage, genomic instability, and embryonic lethality
    Kevin M Hopkins
    Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Mol Cell Biol 24:7235-48. 2004
    ..These investigations establish Mrad9 as a key mammalian genetic element of pathways that regulate the cellular response to DNA damage, maintenance of genomic integrity, and proper embryonic development...
  46. ncbi Bub1 and aurora B cooperate to maintain BubR1-mediated inhibition of APC/CCdc20
    Christopher J Morrow
    Faculty of Sciences, University of Manchester, The Michael Smith Building, Oxford Road, Manchester, M13 9PT, UK
    J Cell Sci 118:3639-52. 2005
    ..This bifurcation in the signalling mechanism may help explain why many tumour cells mount a robust checkpoint response following spindle damage, despite exhibiting chromosome instability...
  47. pmc Generating chromosome instability through the simultaneous deletion of Mad2 and p53
    Aurora A Burds
    Department of Biology, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 102:11296-301. 2005
    ..We conclude that the mitotic checkpoint is not essential for viability per se and that a CIN phenotype can be established in culture through the inactivation of both the Mad2- and p53-dependent checkpoint pathways...
  48. ncbi Requirement of Skp1-Bub1 interaction for kinetochore-mediated activation of the spindle checkpoint
    Katsumi Kitagawa
    Department of Molecular Pharmacology, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105, USA
    Mol Cell 11:1201-13. 2003
    ..We propose that the Skp1-Bub1 interaction is important for transmitting a signal to the spindle checkpoint pathway when insufficient tension is present at kinetochores...
  49. pmc The cell cycle-regulated genes of Schizosaccharomyces pombe
    Anna Oliva
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA
    PLoS Biol 3:e225. 2005
    ....
  50. pmc A random-periods model for expression of cell-cycle genes
    Delong Liu
    Biostatistics Branch, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, Research Triangle Park, NC 27709, USA
    Proc Natl Acad Sci U S A 101:7240-5. 2004
    ..The model permits estimation of and hypothesis testing about biologically meaningful parameters that characterize cycling genes...
  51. pmc Genome-wide in silico identification of transcriptional regulators controlling the cell cycle in human cells
    Ran Elkon
    The David and Inez Myers Laboratory for Genetic Research, Department of Human Genetics, Sackler School of Medicine, and School of Computer Science, Tel Aviv University, Tel Aviv 69978, Israel
    Genome Res 13:773-80. 2003
    ..The methods presented here are general and can be applied to the analysis of transcriptional networks controlling any biological process...
  52. ncbi Crossover/noncrossover differentiation, synaptonemal complex formation, and regulatory surveillance at the leptotene/zygotene transition of meiosis
    G Valentin Börner
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Cell 117:29-45. 2004
    ....
  53. ncbi Activating and silencing the mitotic checkpoint through CENP-E-dependent activation/inactivation of BubR1
    Yinghui Mao
    Ludwig Institute for Cancer Research, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Cell 114:87-98. 2003
    ..This supports bifunctional roles for BubR1 in the checkpoint: an enzymatic one requiring CENP-E-dependent activation of its kinase activity at kinetochores and a stoichiometric one as a direct inhibitor of Cdc20...
  54. pmc The yeast S phase checkpoint enables replicating chromosomes to bi-orient and restrain spindle extension during S phase distress
    Jeff Bachant
    Department of Cell Biology and Neuroscience, University of California, Riverside, Riverside, CA 92521, USA
    J Cell Biol 168:999-1012. 2005
    ..We propose that by promoting replication fork integrity under these conditions Rad53 ensures centromere duplication. Replicating chromosomes can then bi-orient in a cohesin-independent manner to restrain untimely spindle extension...
  55. ncbi Mitosis: a matter of getting rid of the right protein at the right time
    Jonathon Pines
    Wellcome Cancer Research UK Gurdon Institute and Dept of Zoology, Tennis Court Road, Cambridge, UK CB2 1QN
    Trends Cell Biol 16:55-63. 2006
    ....
  56. ncbi New weakly expressed cell cycle-regulated genes in yeast
    Ulrik de Lichtenberg
    Center for Biological Sequence Analysis, BioCentrum, Technical University of Denmark, Building 208, DK 2800 Lyngby, Denmark
    Yeast 22:1191-201. 2005
    ..Several of the gene products are believed to be phosphorylated by Cdc28. For many of these new genes, homologues exist in Schizosaccharomyces pombe and Homo sapiens for which the expression also varies with cell cycle progression...
  57. ncbi Timing and checkpoints in the regulation of mitotic progression
    Patrick Meraldi
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Dev Cell 7:45-60. 2004
    ..We propose that cytosolic Mad2-BubR1 is essential to restrain anaphase onset early in mitosis when kinetochores are still assembling...
  58. ncbi Spindle checkpoint component Mad2 contributes to biorientation of homologous chromosomes
    Marion A Shonn
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02130, USA
    Curr Biol 13:1979-84. 2003
    ....
  59. ncbi S-phase checkpoint controls mitosis via an APC-independent Cdc20p function
    Duncan J Clarke
    The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Cell Biol 5:928-35. 2003
    ..Catastrophic mitosis induced by Cdc20p in S phase occurs even in the absence of core APC components. Thus, Cdc20p can function independently of the APC...
  60. ncbi chk-1 is an essential gene and is required for an S-M checkpoint during early embryogenesis
    Nikolaos Kalogeropoulos
    Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton, UK
    Cell Cycle 3:1196-200. 2004
    ..More specifically, disruption of chk-1 expression resulted in embryo lethality, which was attributed to a defect in an intrinsic S-M checkpoint hence causing premature entry into M-phase...
  61. ncbi Yeast and cancer
    Leland H Hartwell
    Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    Biosci Rep 24:523-44. 2004
    ..The discovery of genes that control cell division in yeast, and their relation to cancer, is reviewed...
  62. pmc RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex
    Michael Chang
    Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada
    EMBO J 24:2024-33. 2005
    ..In addition, rmi1Delta strains fail to fully activate Rad53 upon exposure to DNA-damaging agents, suggesting that Rmi1 is also an important part of the Rad53-dependent DNA damage response...
  63. pmc Function of Cdc2p-dependent Bub1p phosphorylation and Bub1p kinase activity in the mitotic and meiotic spindle checkpoint
    Satoko Yamaguchi
    Cell Cycle Laboratory, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    EMBO J 22:1075-87. 2003
    ..Finally, we show that there is a Bub1p-dependent meiotic checkpoint, which is activated in recombination mutants...
  64. ncbi Attachment and tension in the spindle assembly checkpoint
    Jun Zhou
    Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Cell Sci 115:3547-55. 2002
    ....
  65. ncbi Two yeast forkhead genes regulate the cell cycle and pseudohyphal growth
    G Zhu
    Department of Biochemistry, University of Washington, Seattle 98195 7350, USA
    Nature 406:90-4. 2000
    ..Thus, a cascade of transcription factors operates late in the cell cycle. Finally, the fkh1 fkh2 mutant displays a constitutive pseudohyphal morphology, indicating that Fkh1 and Fkh2 may help control the switch to this mode of growth...
  66. ncbi MEN, destruction and separation: mechanistic links between mitotic exit and cytokinesis in budding yeast
    Foong May Yeong
    Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609 corrected
    Bioessays 24:659-66. 2002
    ....
  67. ncbi p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase
    H Wu
    American Health Foundation, Valhalla, New York, NY 10595, USA
    Oncogene 19:4557-62. 2000
    ..Furthermore, BUBR1 phosphorylates p55CDC in vitro, and the phosphorylation of p55CDC by BUBR1 appears to be correlated with spindle checkpoint activation. Together, our studies strongly suggest that BUBR1 may target APC via p55CDC...
  68. ncbi Chromosome segregation and cancer: cutting through the mystery
    P V Jallepalli
    The Johns Hopkins Oncology Center, Baltimore, Maryland 21231, USA
    Nat Rev Cancer 1:109-17. 2001
    ..However, CIN--a driving force of tumorigenesis--could be the cancer cell's ultimate vulnerability. An important goal is to identify novel anticancer compounds that directly target the mitotic errors at the heart of CIN...
  69. pmc Fission yeast Rad26 is a regulatory subunit of the Rad3 checkpoint kinase
    Tom D Wolkow
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Biol Cell 13:480-92. 2002
    ..T12, a mutant that is proficient for cell cycle arrest, but defective in recovery, suggests that these two responses to checkpoint signals require quantitatively different levels of kinase activity from the Rad3/Rad26 complex...
  70. ncbi ATM prevents the persistence and propagation of chromosome breaks in lymphocytes
    Elsa Callen
    Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 1360, USA
    Cell 130:63-75. 2007
    ..Silencing this checkpoint permits DNA ends produced by V(D)J recombination in a lymphoid precursor to serve as substrates for translocations with chromosomes subsequently damaged by other means in mature cells...
  71. ncbi Can sequencing shed light on cell cycling?
    A W Murray
    Center for Genomics Research, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 409:844-6. 2001
    ..Disappointingly, we discovered a few novel cyclins and no new Cdks or components of the spindle checkpoint, and could shed little light on the organization of the cell cycle...
  72. ncbi A DNA damage response pathway controlled by Tel1 and the Mre11 complex
    T Usui
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, 560 0043, Osaka, Japan
    Mol Cell 7:1255-66. 2001
    ..These findings demonstrate that the diverse functions of the Mre11 complex in the cellular DNA damage response are conserved in mammals and yeast...
  73. pmc MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p, and Mad2p
    K G Hardwick
    Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
    J Cell Biol 148:871-82. 2000
    ..We discuss roles for Mad3p and its interactions with other spindle checkpoint proteins and with Cdc20p, the target of the checkpoint...
  74. pmc Telomere binding of checkpoint sensor and DNA repair proteins contributes to maintenance of functional fission yeast telomeres
    Toru M Nakamura
    Departments of Molecular Biology and Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Genetics 161:1437-52. 2002
    ..Thus, checkpoint sensor and DNA repair proteins contribute to telomere maintenance and protection through their association with telomeres...
  75. pmc Loss of spindle assembly checkpoint-mediated inhibition of Cdc20 promotes tumorigenesis in mice
    Min Li
    Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
    J Cell Biol 185:983-94. 2009
    ..Importantly, Cdc20(+/AAA) mice developed spontaneous tumors at highly accelerated rates, indicating that the SAC-mediated inhibition of Cdc20 is an important tumor-suppressing mechanism...
  76. pmc ORC and the intra-S-phase checkpoint: a threshold regulates Rad53p activation in S phase
    Kenji Shimada
    University of Geneva, Department of Molecular Biology, Switzerland
    Genes Dev 16:3236-52. 2002
    ..Our data suggest the existence of an S-phase-specific threshold that may be necessary to allow cells to tolerate damage-like DNA structures present at normal replication forks...
  77. pmc Linking kinetochore-microtubule binding to the spindle checkpoint
    Daniel J Burke
    Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, 1300 Jefferson Park Avenue, Box 800733, Charlottesville, VA 22908 0733, USA
    Dev Cell 14:474-9. 2008
    ..At least eight protein kinases are implicated in spindle checkpoint signaling, arguing that a traditional signal transduction cascade is integral to spindle checkpoint signaling...
  78. pmc Differential regulation of the cellular response to DNA double-strand breaks in G1
    Jacqueline H Barlow
    Department of Genetics and Development, Columbia University Medical Center, 701 West 168th Street, New York, NY 10032 2704, USA
    Mol Cell 30:73-85. 2008
    ..Together, these results demonstrate that the DNA repair machinery distinguishes between different types of damage in G1, which translates into different modes of checkpoint activation in G1 and S/G2 cells...
  79. ncbi Complexity in the spindle checkpoint
    D J Burke
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia 22906, USA
    Curr Opin Genet Dev 10:26-31. 2000
    ..The checkpoint mechanism involves a signal transduction cascade that is more complex than was originally envisioned...
  80. ncbi Reconstitution and molecular analysis of the hRad9-hHus1-hRad1 (9-1-1) DNA damage responsive checkpoint complex
    M A Burtelow
    Division of Developmental Oncology Research, Department of Molecular Pharmacology, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Biol Chem 276:25903-9. 2001
    ..Collectively, these analyses suggest a model of how these three proteins assemble to form a functional checkpoint complex, which we dubbed the 9-1-1 complex...
  81. ncbi Anaphase onset does not require the microtubule-dependent depletion of kinetochore and centromere-binding proteins
    Julie C Canman
    University of North Carolina, Department of Biology, 607 Fordham Hall, CB3280, Chapel Hill, NC 27599, USA
    J Cell Sci 115:3787-95. 2002
    ....
  82. pmc Cyclebase.org--a comprehensive multi-organism online database of cell-cycle experiments
    Nicholas Paul Gauthier
    Center for Biological Sequence Analysis, BioCentrum DTU, Technical University of Denmark, Building 208, DK 2800 Lyngby, Denmark
    Nucleic Acids Res 36:D854-9. 2008
    ..Cyclebase is available at http://www.cyclebase.org...
  83. ncbi Impaired tRNA nuclear export links DNA damage and cell-cycle checkpoint
    Ata Ghavidel
    Ontario Cancer Institute, Division of Signaling Biology, Toronto, Ontario M5G 1L7, Canada
    Cell 131:915-26. 2007
    ....
  84. ncbi Restarting the cell cycle when the checkpoint comes to a halt
    Marcel A T M van Vugt
    Department of Medical Oncology, University Medical Center Utrecht, Utrecht, The Netherlands
    Cancer Res 65:7037-40. 2005
    ..Here, we discuss these novel insights and discuss their possible implications for the treatment of cancer...
  85. pmc A telomeric repeat sequence adjacent to a DNA double-stranded break produces an anticheckpoint
    Rhett J Michelson
    Molecular and Cellular Biology Department, University of Arizona, Tucson, Arizona 85721, USA
    Genes Dev 19:2546-59. 2005
    ..We propose that after formation of the DSB near the telomeric repeat, a mature telomere forms in 1-2 h, and the telomere then contains proteins that inhibit checkpoint signaling from nearby DNA breaks...
  86. ncbi [Centrosome as an organizing center of intracellular information: insights viewed from the spindle pole of budding yeast]
    Tomohiro Akashi
    Division of Molecular Mycology and Medicine, Center for Neural Disease and Cancer, Nagoya University Graduate School of Medicine, 65 Tsurumai, Nagoya 466 8550, Japan
    Seikagaku 77:261-8. 2005
  87. ncbi [Replication of Epstein-Barr virus and host cell response]
    Tatsuya Tsurumi
    Division of Virology Aichi Cancer Center Research Institute, Kanokoden, Chikusa ku, Nagoya 464 8681, Japan
    Seikagaku 77:1180-4. 2005
  88. pmc The Ulp2 SUMO protease is required for cell division following termination of the DNA damage checkpoint
    David C Schwartz
    Department of Molecular Biophysics, Yale University, New Haven, CT 06520 8114, USA
    Mol Cell Biol 27:6948-61. 2007
    ....
  89. ncbi Checkpoint regulation of replication dynamics in UV-irradiated human cells
    Paul D Chastain
    Department of Pathology and Laboratory Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7525 USA
    Cell Cycle 5:2160-7. 2006
    ..These findings illustrate the concordance of data derived from different experimental approaches, thus strengthening the evidence that the activation of the intra-S checkpoint by UVC is dependent on the ATR and Chk1 kinases...
  90. ncbi Before and after the spindle assembly checkpoint--an APC/C point of view
    Armand de Gramont
    The Laboratory of Molecular and Cellular Biology, The National Institute of Diabetes and Digestive and Kidney Diseases, The National Institutes of Health, Bethesda, Maryland 20892 0840, USA
    Cell Cycle 5:2168-71. 2006
    ....
  91. pmc Modelling the checkpoint response to telomere uncapping in budding yeast
    C J Proctor
    Institute for Ageing and Health, and School of Clinical Medical Sciences Gerontology, Centre for Integrated Systems Biology of Ageing and Nutrition, Newcastle University, Newcastle upon Tyne NE4 6BE, UK
    J R Soc Interface 4:73-90. 2007
    ..The model can be used to carry out a variety of in silico experiments in which different genes are knocked out and the results of simulation are compared to experimental data. Possible extensions to the model are also discussed...
  92. pmc DDB1 maintains genome integrity through regulation of Cdt1
    Courtney A Lovejoy
    Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA
    Mol Cell Biol 26:7977-90. 2006
    ..Therefore, DDB1 prevents DNA lesions from accumulating in replicating human cells, in part by regulating Cdt1 degradation...
  93. ncbi Contribution of DNA repair and cell cycle checkpoint arrest to the maintenance of genomic stability
    Penny A Jeggo
    Genome Damage and Stability Centre, University of Sussex, East Sussex BN1 9RQ, UK
    DNA Repair (Amst) 5:1192-8. 2006
    ..ATM's checkpoint function has a bigger impact on genomic stability but strikingly the two damage response pathways co-operate in a more than additive manner. In contrast, ATM's repair function is important for survival post irradiation...
  94. ncbi Checkpoint deficient rad53-11 yeast cannot accumulate dNTPs in response to DNA damage
    Ahmet Koc
    Izmir Institute of Technology, Department of Molecular Biology and Genetics, 35430 Urla, Izmir, Turkey
    Biochem Biophys Res Commun 353:527-30. 2007
    ..Thus, the results indicate that Rad53 checkpoint function is not required for dNTP pool maintenance in normally growing cells, but is required for dNTP pool expansion in cells exposed to DNA-damaging agents...
  95. pmc Drosophila ATR in double-strand break repair
    Jeannine R Larocque
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Genetics 175:1023-33. 2007
    ..However, this does not account for all of the defects we observed. We propose a novel role for MEI-41 in DSB repair, independent of the Chk1/Chk2-mediated checkpoint response...
  96. ncbi Docking onto chromatin via the Saccharomyces cerevisiae Rad9 Tudor domain
    Muriel Grenon
    Genome Stability Laboratory, Department of Biochemistry and National Centre for Biomedical Engineering Science, National University of Ireland Galway, Galway, Ireland
    Yeast 24:105-19. 2007
    ..The study further shows that the function of this interaction is cell cycle-regulated; the role in checkpoint activation is restricted to the G(1) phase and its role in DNA repair is restricted to G(2)...
  97. ncbi Therapeutic opportunities to control tumor cell cycles
    Marcos Malumbres
    Cell Division and Cancer Group, Centro Nacional de Investigaciones Oncologicas CNIO, Madrid, Spain
    Clin Transl Oncol 8:399-408. 2006
    ..Current research in this area will undoubtedly result in new and improved targeted therapies for cancer treatment...
  98. ncbi Rad3-dependent phosphorylation of the checkpoint clamp regulates repair-pathway choice
    Mihoko Kai
    Department of Pathology, Stanford University School of Medicine, Stanford, California 94305 5324, USA
    Nat Cell Biol 9:691-7. 2007
    ..Our findings reveal a new mechanism by which phosphorylation of Rad9 at Thr 225 regulates the choice of repair pathways for maintaining genomic integrity during the cell cycle...
  99. ncbi The role of NBS1 in DNA double strand break repair, telomere stability, and cell cycle checkpoint control
    Ying Zhang
    Department of Environmental and Radiological Health Science, Colorado State University, Fort Collins, 80523, USA
    Cell Res 16:45-54. 2006
    ..Emphasis is placed on the role of NBS1 in the DNA double strand repair, modulation of the DNA damage sensing and signaling, cell cycle checkpoint control and maintenance of telomere stability...
  100. ncbi A novel cell cycle inhibitor stalls replication forks and activates S phase checkpoint
    Vaidehi Krishnan
    Institute of Molecular and Cell Biology, Proteos, Singapore
    Cell Cycle 6:1621-30. 2007
    ..Thus, ENA is a potent cell cycle inhibitor with conceivable therapeutic potential...
  101. ncbi Roles of Saccharomyces cerevisiae RAD17 and CHK1 checkpoint genes in the repair of double-strand breaks in cycling cells
    Nelson Bracesco
    Lab Radiobiología, Departamento Biofísica, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Radiat Environ Biophys 46:401-7. 2007
    ....

Research Grants63

  1. REGULATION OF CYCLIN/CDK ACTIVITY IN YEAST
    Raymond Deshaies; Fiscal Year: 2000
    ..A large group of CDC genes that promote different stages in the cell division cycle pathway have been identified by the isolation of yeast ..
  2. PEPTIDE NUCLEIC ACIDS (PNA)-MEDIATED GENE EXPRESSION
    Gan Wang; Fiscal Year: 2003
    ..To investigate parameters that affect PNA delivery efficiency and intracellular PNA binding. To explore the possibility of PNA binding-induced endogenous human gene expression. ..
  3. INTERSTRAND CROSSLINKING REPAIR AND DNA RECOMBINATION
    Lei Li; Fiscal Year: 2005
    ..abstract_text> ..
  4. Analysis of mammalian cell cycle checkpoint genes
    Lei Li; Fiscal Year: 2007
    ..Our results are expected to further elucidate the mechanisms of the DNA damage and replication checkpoint, and the molecular basis of genomic instability at large. ..
  5. Cell Growth and Radiation Survival in Drosophila
    Tin Tin Su; Fiscal Year: 2007
    ..Understanding radiation survival in a multicellular context could help identify potential therapies for when we do not want cells to survive irradiation, such as in the treatment of cancer. ..
  6. TRANCRIPTION-COUPLED DNA REPAIR AND GENETIC INSTABILITY
    Gan Wang; Fiscal Year: 2004
    ....
  7. Cell Cycle Regulation by Checkpoints in Drosophila
    Tin Tin Su; Fiscal Year: 2006
    ..Aim 4) We will screen through existing mutagensensitive Drosophila mutants for their ability to regulate mitosis, S phase and cell death after irradiation, in order to uncover novel genes needed for proper response to DNA damage. ..
  8. An in vivo Screen for Radiation Sensitizers
    Tin Tin Su; Fiscal Year: 2008
    ..Successful completion of this project would establish a novel, rapid and effective primary screen in vivo for radiosensitizers with potential therapeutic value. [unreadable] [unreadable] [unreadable]..
  9. Liver Biology, Development and Disease
    KLAUS KAESTNER; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  10. RNAi in DNA Sequence Elimination in Tetrahymena
    Martin A Gorovsky; Fiscal Year: 2010
    ..These studies should shed light on other RNAi- like processes that are important for normal development and maintenance of genome stability in multicellular organisms. ..
  11. RECOMBINATIONAL TELOMERE MAINTENANCE
    Michael J McEachern; Fiscal Year: 2010
    ....
  12. Activating liver carcinogens in yeast by expressing CYP450 polymorphisms
    Michael Fasullo; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  13. Mitochondria and Carcinogenesis
    Keshav K Singh; Fiscal Year: 2010
    ..The proposed study will help determine the contribution of mutant mtDNA to the development of human cancer. ..
  14. Gene Expression Profiles of Retinal Degeneration
    Constance Cepko; Fiscal Year: 2007
    ..We further plan to characterize the expression patterns of such genes in normal and pathological tissue. Finally, we will explore the function of some of these genes using genetic approaches in mice. [unreadable] [unreadable]..
  15. Molecular Analysis of the Kinetochore
    SONI SHIMODA; Fiscal Year: 2006
    ..A characterization of the proteins involved in chromosome segregation and checkpoint activation could lead to a greater understanding of how human diseases, such as cancer, can be prevented. [unreadable] [unreadable]..
  16. P27Kip1 in Hepatocyte Proliferation
    Liang Zhu; Fiscal Year: 2006
    ..abstract_text> ..
  17. MECHANISTIC STUDIES OF DNA MISMATCH REPAIR
    A Lien Lu Chang; Fiscal Year: 2006
    ..Particularly, alterations in protein-protein interactions under oxidative stress will be investigated. These studies should advance our understanding of the role of DNA repair in genome stability and tumor susceptibility. ..
  18. Sixth International Conference on Systems Biology (ICSB 2005)
    Andrew Murray; Fiscal Year: 2005
    ..The speakers and contributors will include experimental biologists, chemists, computer scientists, physicists, engineers, and mathematicians. ..
  19. Experimental and Computational Studies of Exit from Mitosis in Budding Yeast
    John J Tyson; Fiscal Year: 2010
    ..g., embryonic development, tissue regeneration, wound healing, and carcinogenesis. ..
  20. Cholesterol and amyloidogenesis
    Kumar Sambamurti; Fiscal Year: 2007
    ..These hypotheses are amenable to testing as outlined in the corresponding sections of the proposal and their study will advance our understanding of the pathogenesis of Alzheimer's disease. ..
  21. The Role of Chfr In Tumorigenesis
    Zheng Fu; Fiscal Year: 2007
    ..abstract_text> ..
  22. FUNCTIONAL GENOMICS OF THE BETA-CELL
    KLAUS KAESTNER; Fiscal Year: 2007
    ..The new resources generated through this project will be made available to the NIDDK-funded biotechnology centers and the diabetes research community at large. ..
  23. The Role of ABC Transporters in Stem Cells
    Brian Sorrentino; Fiscal Year: 2008
    ..Altogether, these studies should further elucidate the role of the Abcg2 transporter in normal and malignant stem cells, and should further increase our understanding of the role of ABC transporters in stem cells. ..
  24. Function & Regulation of two Novel PARPs, tankyrase 1&2
    Carolyn Price; Fiscal Year: 2008
    ..Aim 4 will investigate the in vivo regulation of tankyrase PARP activity. We will examine the in vivo levels of tankyrase poly(ADP-ribosyl)ation and whether this is regulated by phosphorylation. ..
  25. Cell Cycle Variants and Metastatic Prostate Cancer Risk
    Adam Kibel; Fiscal Year: 2009
    ..At the conclusion of this study, we will have a panel of markers that can be used to predict which patients are at high risk of treatment failure and eventual death from prostate carcinoma. ..
  26. MOUSE MODELS OF DNA REPAIR - DEFECTIVE HUMAN DISEASES
    Errol Friedberg; Fiscal Year: 2011
    ..abstract_text> ..
  27. Study of genomic instability caused by HPV16 E6 and E7
    Dennis McCance; Fiscal Year: 2008
    ..abstract_text> ..
  28. Repair of Oxidatively Damaged Guanines
    A Lien Lu Chang; Fiscal Year: 2008
    ..We anticipate that these studies will advance our understanding of carcinogenesis process and form the background work for the development of new anti-cancer drugs. ..
  29. Development of C57BL/6 ES cell technology for high thoughput use.
    KLAUS KAESTNER; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  30. Algorithms to investigate transcriptional networks
    Sridhar Hannenhalli; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  31. Cosuppression of Ty1 Retrotransposons in S. Cerevisiae
    Yi Wei Jiang; Fiscal Year: 2008
    ..genes be transcriptionally cosuppressed? What yeast genes are required for Tyl cosuppression? Which molecular pathways or processes are involved in Tyl cosuppression? How are Tyl-related genes targeted by cosuppression? ..
  32. FUNCTIONAL ROLE OF MBP 1
    Ratna Ray; Fiscal Year: 2006
    ..To investigate the mechanism by which it induces apoptosis To test the biological effect of MBP-1 disruption in KO mice To determine whether adenovirus-mediated MBP-1 gene transfer can regress tumor growth in vivo. ..
  33. P27kip1 as an early effector of pRB function
    Liang Zhu; Fiscal Year: 2004
    ....
  34. SISTER CHROMATID LINKAGE AND SEPARATION
    Andrew Murray; Fiscal Year: 2004
    ..This information will be directly relevant to understanding how aneuploidy is generated in Down syndrome and tumor progression. ..
  35. A TWO-HYBRID SYSTEM FOR USE IN NON-DIVIDING YEAST CELL
    Margaret Werner Washburne; Fiscal Year: 2002
    ..Once established for yeast, this method should be useful in a broad range of cell types to facilitate the genomic-scale analysis of protein-protein interactions during all phases of growth. ..
  36. DNA REPAIR AND CANCER PRONE HEREDITARY HUMAN DISEASE
    Errol Friedberg; Fiscal Year: 2002
    ....
  37. S6 Phosphorylation & Tyrosine Protein Kinases
    JAMES MALLER; Fiscal Year: 2004
    ..Experiments will evaluate whether Bub1 mediates CSF arrest by the MAPK pathway. This work provides a link between the MAPK/Rsk pathway and checkpoint control of the cell cycle at the metaphase/anaphase transition. ..
  38. IDENTITY AND FUNCTION OF VERTEBRATE TELOMERE PROTEINS
    Carolyn Price; Fiscal Year: 2002
    ..We will use a retroviral cDNA expression library to screen for genes that act as high copy suppressors of defects in telomere function. ..
  39. Time Series Analysis of Expression Profiles
    GREGORY DEWEY; Fiscal Year: 2003
    ..Integral to this effort is the interplay between statistical analysis and experimental design. Such interplay of efforts is made possible by the collaborations built into the project. ..
  40. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 2003
    ..Further work will examine the regulation, localization, and targets of PIx1 and identify kinases that activate xPIkk1. ..
  41. FUNCTION OF A GROWTH INDUCED GENE IN LIVER REGENERATION
    KLAUS KAESTNER; Fiscal Year: 2003
    ....
  42. Problem Solving Environment for Modeling the Cell Cycle
    John Tyson; Fiscal Year: 2002
    ..and some outstanding collaborators (Sible and Cross, experimental cell cycle research; Kitano and Bolouri, software development for molecular cell biology; and Kohn, bioinformatics of mammalian cell cycle controls). ..
  43. CYCLIN E EXPRESSION IN HUMAN CANCER
    Peggy Porter; Fiscal Year: 2002
    ..abstract_text> ..
  44. Quality Threshold Analysis of Prostate Cancers
    Carlos Moreno; Fiscal Year: 2004
    ..Identification of genetic profiles associated with prostatic neoplasias that are more likely to result in aggressive disease may lead to improved management of prostate cancer patients. ..
  45. GENE EXPRESSION IN SINGLE, IDENTIFIED CNS CELLS
    Constance Cepko; Fiscal Year: 2001
    ..abstract_text> ..
  46. IDENTIFICATION OF A 12P PROSTATE TUMOR SUPPRESSOR GENE
    Adam Kibel; Fiscal Year: 2006
    ..The identification of a novel tumor suppressor would provide a target for better prostate cancer therapy and/or more accurate prognostic information for men newly diagnosed with the disease. ..
  47. CONTROL OF GENE ACTIVITY AND ORGANELLE BIOGENESIS
    MARTIN GOROVSKY; Fiscal Year: 2006
    ..abstract_text> ..
  48. A compendium of gene expression in stationary phase
    Margaret Werner Washburne; Fiscal Year: 2005
    ..parent abstract not available ..
  49. Molecular Alterations in Lobular/Ductal Breast Cancer
    Peggy Porter; Fiscal Year: 2005
    ..abstract_text> ..
  50. MENTORING FOR NEW CANCER THERAPEAUTICS
    Gary Schwartz; Fiscal Year: 2005
    ..This K24 award mechanism represents the essence of what we are proposing and for which support is needed. ..
  51. Computation tools for analyzing gene expression data
    Naama Barkai; Fiscal Year: 2004
    ..Kinetic microarray experiments that highlight specific features of the transcriptional network will be designed, performed and analyzed. Methods for extracting regulatory relationships from these experiments will be developed. ..
  52. MOLECULAR AND GENETIC BASIS OF CELL PROLIFERATION
    MICHAEL TYERS; Fiscal Year: 2005
    ..The Gordon Conferences format provides a unique environment that encourages informal and open discussion among the participants and this in turn fosters initiation of collaborative efforts and stimulates future research directions. ..
  53. DNA REPAIR AND ITS RELATIONSHIP TO CARCINOGENESIS
    Errol Friedberg; Fiscal Year: 2001
    ..There is reason to believe that the proteins encoded by these genes operate in a different chromatin-modulating complex, and it is proposed to isolate and characterize this putative complex as well. ..
  54. SPINDLE ASSEMBLY CHECKPOINT IN VERTEBRATES AND YEAST
    Rey Huei Chen; Fiscal Year: 2003
    ..The knowledge will then be applied to the question of how the deregulation of the checkpoint function may contribute to cancer progression. ..
  55. STEM CELLS IN TRANSGENIC MOUSE MAMMARY TUMORIGENESIS
    Robert Dickson; Fiscal Year: 2002
    ....
  56. Phase II Study of Imatinib Mesylate in Patients with Inoperable Melanoma
    Gary K Schwartz; Fiscal Year: 2010
    ..Dose reductions are allowed in the setting of toxicity. Imaging studies will be performed on an every 6 week schedule. ..
  57. 2003 Genetic Toxicology Gordon Conference
    Penny Jeggo; Fiscal Year: 2003
    ..We particularly air to obtain funding to support applications from minority groupings and younger scientists. We have already identified a significant participation of women amongst our list of speakers. ..
  58. ROLE OF FRAGILE SITES IN CHROMOSOME BREAKAGE AND CANCER
    Thomas Glover; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  59. Conference Proposal to support FASEB Conference on Ciliate Molecular Biology
    Carolyn Price; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  60. Smt4 Regulation of Centromere Function and Cohesion
    Jeff Bachant; Fiscal Year: 2007
    ..These studies will elucidate potentially conserved mechanisms controlling chromosome segregation and identify additional Smt3/SUMO-1 substrates regulating centromere function. ..
  61. EFFECT OF HPV-16 E7 ON DIFFERENTIATING EPITHELIAL CELLS
    Dennis McCance; Fiscal Year: 2005
    ..Additionally, we will investigate the ability of E7 to increase cyclin/kinase activity in differentiating cells, which also contain high levels of kinase inhibitors. ..
  62. RADIATION INDUCTION OF GENOMIC REARRANGEMENTS IN YEAST
    Michael Fasullo; Fiscal Year: 2004
    ..abstract_text> ..
  63. Signaling by the ATM and ATR Kinases
    David Cortez; Fiscal Year: 2006
    ..The award will facilitate the candidate's transition from a mentored research environment to an independent academic research position focused on basic cancer biology. ..