Genomes and Genes
Summary: The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.
Publications267 found, 100 shown here
- Crystal structure of the lactose operon repressor and its complexes with DNA and inducerM Lewis
Johnson Research Foundation, University of Pennsylvania, Philadelphia 19104, USA
Science 271:1247-54. 1996The lac operon of Escherichia coli is the paradigm for gene regulation. Its key component is the lac repressor, a product of the lacI gene...
- Multistability in the lactose utilization network of Escherichia coliErtugrul M Ozbudak
Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Nature 427:737-40. 2004..The phase diagram thus serves as a sensitive probe of molecular interactions and as a powerful tool for rational network design...
- Optimality and evolutionary tuning of the expression level of a proteinErez Dekel
Department of Molecular Cell Biology and Department of Physics of Complex Systems, The Weizmann Institute of Science, Rehovot 76100, Israel
Nature 436:588-92. 2005..Thus, protein expression from the lac operon seems to be a solution of a cost-benefit optimization problem, and can be rapidly tuned by evolution to function ..
- The cost of expression of Escherichia coli lac operon proteins is in the process, not in the productsDaniel M Stoebel
Department of Ecology and Evolution, Stony Brook University, Stony Brook, New York 11794, USA
Genetics 178:1653-60. 2008..Our results identify the basis of a single selective pressure likely acting across the entire E. coli transcriptome...
- Transcriptional infidelity promotes heritable phenotypic change in a bistable gene networkAlasdair J E Gordon
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA
PLoS Biol 7:e44. 2009..We used a classic bistable memory module to monitor and capture transient RNA errors: the lac operon of Escherichia coli comprises an autocatalytic positive feedback loop producing a heritable all-or-none ..
- A stochastic single-molecule event triggers phenotype switching of a bacterial cellPaul J Choi
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Science 322:442-6. 2008..single-molecule sensitivity, we investigated the molecular mechanism of how an Escherichia coli cell with the lac operon switches from one phenotype to another...
- Interplay of protein and DNA structure revealed in simulations of the lac operonLuke Czapla
Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America
PLoS ONE 8:e56548. 2013..The short loops implicated in the regulation of the lac operon suggest the involvement of factors other than DNA and repressor in gene control...
- Comparison of deterministic and stochastic models of the lac operon genetic networkMichail Stamatakis
Department of Chemical and Biomolecular Engineering, Rice University, Houston, Texas 77005, USA
Biophys J 96:887-906. 2009The lac operon has been a paradigm for genetic regulation with positive feedback, and several modeling studies have described its dynamics at various levels of detail...
- Lysophosphatidylcholine as an adjuvant for lentiviral vector mediated gene transfer to airway epithelium: effect of acyl chain lengthPatricia Cmielewski
Dept of Respiratory and Sleep Medicine, Women s and Children s Hospital, 72 King William Rd, North Adelaide, SA 5006, Australia
Respir Res 11:84. 2010..We examined the electrophysiological and physical effect of airway pre-treatment with variants of LPC on lentiviral (LV) vector gene transfer efficiency in murine nasal airways in vivo...
- Analysis of in-vivo LacR-mediated gene repression based on the mechanics of DNA loopingYongli Zhang
Departments of Chemistry and Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, United States of America
PLoS ONE 1:e136. 2006....
- Unequal fidelity of leading strand and lagging strand DNA replication on the Escherichia coli chromosomeI J Fijalkowska
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02 106 Warsaw, Pawinskiego 5A, Poland
Proc Natl Acad Sci U S A 95:10020-5. 1998..We have constructed a series of lacZ strains in which the lac operon is inserted into the bacterial chromosome in the two possible orientations with regard to the chromosomal ..
- A switch from high-fidelity to error-prone DNA double-strand break repair underlies stress-induced mutationRebecca G Ponder
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
Mol Cell 19:791-804. 2005..Our findings reveal an RpoS-controlled switch from high-fidelity to mutagenic DSBR under stress. This limits genetic instability both in time and to localized genome regions, potentially important evolutionary strategies...
- Isolation and characterization of RNA polymerase rpoB mutations that alter transcription slippage during elongation in Escherichia coliYan Ning Zhou
National Cancer Institute, National Institutes of Health, Frederick National Laboratory for Cancer Research, Frederick, Maryland 21702, USA
J Biol Chem 288:2700-10. 2013..Our E. coli study complements an accompanying study of slippage by yeast RNAP II and provides the basis for future studies on the mechanism of transcription fidelity...
- Genetic analysis of prokaryotic and eukaryotic DNA-binding proteins in Escherichia coliF W Whipple
Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
Nucleic Acids Res 26:3700-6. 1998..coli strains bearing reporters for other types of genetic systems, including repression-based and activation-based systems in which chimeric proteins are used to examine interactions between foreign protein domains...
- Operator sequence alters gene expression independently of transcription factor occupancy in bacteriaHernan G Garcia
Department of Physics, California Institute of Technology, Pasadena, CA 91125, USA
Cell Rep 2:150-61. 2012....
- SOS mutator DNA polymerase IV functions in adaptive mutation and not adaptive amplificationG J McKenzie
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030 3411, USA
Mol Cell 7:571-9. 2001..coli lac operon. A nonpolar dinB mutation reduces adaptive mutation frequencies by 85% but does not affect adaptive ..
- Evidence for fusion between cardiac and skeletal muscle cellsHans Reinecke
Department of Pathology, University of Washington, Box 357470, HSB Room D 514 Seattle, Wash 98195 7470, USA
Circ Res 94:e56-60. 2004..The full text of this article is available online at http://circres.ahajournals.org...
- Assaying promoter activity using LacZ and GFP as reportersPaul Carroll
Institute of Cell and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, 4 Newark Street, Whitechapel, London E1 2AT, UK
Methods Mol Biol 465:265-77. 2009..This chapter describes two reporter systems (GFP and LacZ) used for determining promoter activity that have been widely used in mycobacteria...
- Bacterial repression loops require enhanced DNA flexibilityNicole A Becker
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
J Mol Biol 349:716-30. 2005The Escherichia coli lac operon provides a classic paradigm for understanding regulation of gene transcription...
- Development of new inbred transgenic strains of rats with LacZ or GFPHirokazu Inoue
Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, Tochigi 329 0498, Japan
Biochem Biophys Res Commun 329:288-95. 2005..Our Tg-rat system may provide great benefits for the elucidation of the cellular process of regenerative medicine, including cell and tissue transplantation...
- Cost-benefit theory and optimal design of gene regulation functionsTomer Kalisky
Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
Phys Biol 4:229-45. 2007..We apply this theory to the well-studied lac operon of E. coli...
- The mutY gene: a mutator locus in Escherichia coli that generates G.C----T.A transversionsY Nghiem
Molecular Biology Institute, University of California, Los Angeles 90024
Proc Natl Acad Sci U S A 85:2709-13. 1988..coli. The mutY locus may act in a similar but reciprocal fashion to the previously characterized mutT locus, which results in A.T----C.G transversions...
- Interferon-gamma induced medulloblastoma in the developing cerebellumWensheng Lin
Jack Miller Center for Peripheral Neuropathy, Department of Neurology, University of Chicago, Chicago, Illinois 60637, USA
J Neurosci 24:10074-83. 2004..Thus, IFN-gamma expression in the perinatal period can induce SHH expression and medulloblastoma in the cerebellum by a STAT1-dependent mechanism, and its continued presence appears to promote a host response to the tumor...
- Bactericidal antisense effects of peptide-PNA conjugatesL Good
Center for Genomics Research, Karolinska Institute, Berzelius väg 37, 171 77, Stockholm, Sweden
Nat Biotechnol 19:360-4. 2001..These results indicate that peptides can be used to carry antisense PNA agents into bacteria. Such peptide-PNA conjugates open exciting possibilities for anti-infective drug development and provide new tools for microbial genetics...
- Failure of blood-island formation and vasculogenesis in Flk-1-deficient miceF Shalaby
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
Nature 376:62-6. 1995..These results indicate that Flk-1 is essential for yolk-sac blood-island formation and vasculogenesis in the mouse embryo...
- Pre-dispositions and epigenetic inheritance in the Escherichia coli lactose operon bistable switchLydia Robert
INSERM, and Faculty of Medicine, Paris Descartes University, 24 Rue de Faubourg St Jacques, Paris, France
Mol Syst Biol 6:357. 2010..Thus, stochasticity at the local level of the network and global physiology are synergistically involved in cell response determination...
- Activation of the receptor tyrosine kinase Kit is required for the proliferation of melanoblasts in the mouse embryoM A MacKenzie
MRC Human Genetics Unit, Western General Hospital, Edinburgh, EH4 2XU, United Kingdom
Dev Biol 192:99-107. 1997..Our data do not describe a dependence upon Kit for melanoblast migration or differentiation...
- Matrix metalloproteinase-9 is required for adequate angiogenic revascularization of ischemic tissues: potential role in capillary branchingChad Johnson
Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology Emory University School of Medicine, Atlanta, GA, USA
Circ Res 94:262-8. 2004..Our study indicates that angiogenesis triggered by tissue ischemia requires MMP-9, which may be involved in capillary branching, a potential novel role for this MMP that could be exploited to control angiogenesis...
- High local concentration: a fundamental strategy of lifeStefan Oehler
Biomedical Sciences Research Center Alexander Fleming, 34 Fleming Street, GR 16672 Vari, Greece
J Mol Biol 395:242-53. 2010..Local increase in concentration leads to cooperative or competitive interactions between molecules. It is an important principle of life...
- Development of genetic circuitry exhibiting toggle switch or oscillatory behavior in Escherichia coliMariette R Atkinson
Department of Biological Chemistry, University of Michigan School of Medicine, Ann Arbor, MI 48109, USA
Cell 113:597-607. 2003..For the toggle switch, LacI was provided constitutively; the level of active repressor was controlled by using a lacY mutant and varying the concentration of IPTG. This circuitry provided nearly discontinuous expression of activator...
- Novel Streptococcus infantarius subsp. infantarius variants harboring lactose metabolism genes homologous to Streptococcus thermophilusChristoph Jans
Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland
Food Microbiol 31:33-42. 2012..7-100% sequence identity to S. infantarius subsp. infantarius ATCC BAA-102(T) and a gal-lac operon with 91.7-97.6% sequence identity to S. thermophilus, absent in all sequenced SBSEC strains analyzed...
- In vivo tests of thermodynamic models of transcription repressor functionSudheer Tungtur
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS 66160, United States
Biophys Chem 159:142-51. 2011..Further analysis of each system suggests that the observed discrepancies between in vitro and in vivo results reflect the contributions of additional equilibria to the transcription repression process...
- Organ-specific increase in mutation accumulation and apoptosis rate in CuZn-superoxide dismutase-deficient miceRita A Busuttil
Departments of Physiology and Molecular Medicine, University of Texas Health Science Center, San Antonio, 78240, USA
Cancer Res 65:11271-5. 2005..5-fold was found not earlier than at 6 months. No increased mutation accumulation was observed in brain or spleen. These results support the hypothesis, that oxidative stress is an important causal factor of cancer in mammals...
- The tandem inversion duplication in Salmonella enterica: selection drives unstable precursors to final mutation typesElisabeth Kugelberg
Department of Microbiology, College of Biological Sciences, University of California, Davis, CA 95616, USA
Genetics 185:65-80. 2010..Growth improves with each step in formation of an SJ or aTID amplification, allowing selection to favor completion of the mutation process...
- The outflow tract of the heart is recruited from a novel heart-forming fieldC H Mjaatvedt
Department of Cell Biology and Anatomy, Medical University of South Carolina, 171 Ashley Avenue, Charleston, South Carolina 29425, USA
Dev Biol 238:97-109. 2001..This new concept of the heart outlet's embryonic origin provides a new basis for explaining a variety of gene-expression patterns and cardiac defects described in both transgenic animals and human congenital heart disease...
- Interconvertible lac repressor-DNA loops revealed by single-molecule experimentsOi Kwan Wong
Department of Biochemistry, Brandeis University, Waltham, Massachusetts, USA
PLoS Biol 6:e232. 2008..The ability to switch between different loop structures may help to explain how robust transcription regulation is maintained even though the mechanical work required to form a loop may change substantially with metabolic conditions...
- Mutability and importance of a hypermutable cell subpopulation that produces stress-induced mutants in Escherichia coliCaleb Gonzalez
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America
PLoS Genet 4:e1000208. 2008..e., it is important. We consider a model in which HMS differentiation is controlled by stress responses. Differentiation of an HMS potentially limits the risks of mutagenesis in cell clones...
- Multiple developmental roles of VEGF suggested by a LacZ-tagged alleleL Miquerol
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G1X5, Canada
Dev Biol 212:307-22. 1999..We believe that the LacZ-tagged allele we have generated offers a precise means of detecting VEGF expression under a variety of physiological and pathological conditions...
- Efficient Cre-mediated deletion in cardiac progenitor cells conferred by a 3'UTR-ires-Cre allele of the homeobox gene Nkx2-5Edouard G Stanley
Victor Chang Cardiac Research Institute, St Vincent s Hospital, Darlinghurst, NSW, Australia
Int J Dev Biol 46:431-9. 2002..Our data suggest that the Nkx2-5IRESCre strain will be useful for genetic dissection of the multiple tiers of lineage allocation to the forming heart as well as of molecular interactions within the heart fields and heart tube...
- Gcm2 and Foxn1 mark early parathyroid- and thymus-specific domains in the developing third pharyngeal pouchJ Gordon
Centre for Genome Research Institute of Cell, Animal and Population Biology, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
Mech Dev 103:141-3. 2001..25. Our results show that Gcm2 and Foxn1 expression mark two complementary domains that prefigure parathyroid and thymus regions within the common primordium before morphological distinctions are present...
- A focused and efficient genetic screening strategy in the mouse: identification of mutations that disrupt cortical developmentKonstantinos Zarbalis
Department of Neurology and the Ernest Gallo Clinic and Research Center, University of California at San Francisco, Emeryville, USA
PLoS Biol 2:E219. 2004..Our results also highlight the utility of focused screens in the mouse, in addition to the large-scale and broadly targeted screens that are being carried out at mutagenesis centers...
- Optimality and evolution of transcriptionally regulated gene expressionFrank J Poelwijk
AMOLF Institute, Science Park 104, 1098 XG, Amsterdam, The Netherlands
BMC Syst Biol 5:128. 2011..The cost and benefit of gene expression are the driving factors. While the former can be determined by gratuitous induction, the latter is difficult to measure directly...
- The effect of stochasticity on the lac operon: an evolutionary perspectiveMilan van Hoek
Theoretical Biology Bioinformatics Group, Utrecht University, Utrecht, The Netherlands
PLoS Comput Biol 3:e111. 2007..In some systems, noise in gene expression has been quantified, in among others the lac operon of Escherichia coli...
- Whole mount enzyme histochemistry as a rapid screen at necropsy for expression of beta-galactosidase (LacZ)-bearing transgenes: considerations for separating specific LacZ activity from nonspecific (endogenous) galactosidase activityBrad Bolon
GEMpath Inc, Cedar City, Utah 84720 8400, USA
Toxicol Pathol 36:265-76. 2008....
- Boolean models can explain bistability in the lac operonAlan Veliz-Cuba
Department of Mathematics, University of Nebraska Lincoln, Lincoln, Nebraska, USA
J Comput Biol 18:783-94. 2011The lac operon in Escherichia coli has been studied extensively and is one of the earliest gene systems found to undergo both positive and negative control...
- The lac operon galactoside acetyltransferaseSteven L Roderick
Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
C R Biol 328:568-75. 2005Of the proteins encoded by the three structural genes of the lac operon, the galactoside acetyltransferase (thiogalactoside transacetylase, LacA, GAT) encoded by lacA is the only protein whose biological role remains in doubt...
- Cooperation between translating ribosomes and RNA polymerase in transcription elongationSergey Proshkin
Department of Biochemistry, New York University School of Medicine, New York, NY 10016, USA
Science 328:504-8. 2010..Such a cooperative mechanism ensures that the transcriptional yield is always adjusted to translational needs at different genes and under various growth conditions...
- Secrets of the lac operon. Glucose hysteresis as a mechanism in dietary restriction, aging and diseaseCharles V Mobbs
Departments of Neuroscience and Geriatrics, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
Interdiscip Top Gerontol 35:39-68. 2007..toxic effects of glucose are suggested by two general principles of metabolic processes, illustrated by the lac operon but also observed with glucose-induced gene expression...
- Stochastic switching in gene networks can occur by a single-molecule event or many molecular stepsPaul J Choi
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
J Mol Biol 396:230-44. 2010..Finally, we apply our methods to recent experimental data on switching in Escherichia coli lactose metabolism, providing quantitative interpretations of a single-molecule switching mechanism...
- Tyrosinase-related protein 2 promoter targets transgene expression to ocular and neural crest-derived tissuesS Zhao
Department of Cell Biology, Baylor College of Medicine, Houston, Texas, 77030, USA
Dev Biol 216:154-63. 1999..These studies demonstrate that the TRP2 promoter is valuable for transgenic studies of ocular differentiation and development of neural crest cells...
- Photoacoustic imaging of lacZ gene expression in vivoLi Li
Washington University in St Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, St Louis, Missouri 63130, USA
J Biomed Opt 12:020504. 2007..With the future development of new absorption-based reporter gene systems, it is anticipated that photoacoustic technology will provide a valuable tool for molecular imaging research...
- Adaptive mutation in Escherichia coliPatricia L Foster
Department of Biology, Indiana University, Jordan Hall, 1001 East Third St, Bloomington, IN 47405, USA
J Bacteriol 186:4846-52. 2004
- Myogenic determination occurs independently in somites and limb budsB Kablar
Institute for Molecular Biology and Biotechnology, Cancer Research Group, McMaster University, Hamilton, Ontario, L8S 4K1, Canada
Dev Biol 206:219-31. 1999..Therefore, cells are recruited into the MyoD-dependent myogenic lineage through activation of the -20-kb MyoD enhancer and this occurs independently in somites and limb buds...
- Tbr1 regulates differentiation of the preplate and layer 6R F Hevner
Nina Ireland Laboratory of, Developmental Neurobiology, Department of Psychiatry, University of California, San Francisco, San Francisco, CA 94143, USA
Neuron 29:353-66. 2001..These results show that Tbr1 is a common genetic determinant for the differentiation of early-born glutamatergic neocortical neurons and provide insights into the functions of these neurons as regulators of cortical development...
- The regulation of MyoD gene expression: conserved elements mediate expression in embryonic axial muscleA Asakura
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA
Dev Biol 171:386-98. 1995..The functional conservation of the regulatory region of the mouse myoD gene in Xenopus embryos suggests that the regulatory pathway that activates myoD gene transcription in axial muscles is conserved among vertebrates...
- Enhancement of DNA flexibility in vitro and in vivo by HMGB box A proteins carrying box B residuesNadia T Sebastian
Department of Chemistry, Creighton University, 2500 California Place, Omaha, Nebraska 68178, USA
Biochemistry 48:2125-34. 2009..These results demonstrate important roles for cationic leader amino acids in HMGB folding, DNA interaction, and DNA bending...
- The mouse Gtl2 gene is differentially expressed during embryonic development, encodes multiple alternatively spliced transcripts, and may act as an RNAK Schuster-Gossler
Jackson Laboratory, Bar Harbor, Maine, USA
Dev Dyn 212:214-28. 1998..Gtl2 transcript levels are present in parthenogenic embryos but may be reduced, consistent with the pattern of inheritance of the Gtl2lacZ phenotype...
- Demand theory of gene regulation. II. Quantitative application to the lactose and maltose operons of Escherichia coliM A Savageau
Department of Microbiology and Immunology, The University of Michigan, Ann Arbor, Michigan 48109 0620, USA
Genetics 149:1677-91. 1998..Finally, the theory predicts an optimum value of C that is in agreement with the observed frequency for E. coli colonizing the human intestinal tract...
- Counteraction by MutT protein of transcriptional errors caused by oxidative damageF Taddei
Institut Jacques Monod, Tour 43, 2 place Jussieu, 75251 Paris Cedex 05, France
Science 278:128-30. 1997..Such control of transcriptional fidelity by the ubiquitous MutT function has implications for evolution of RNA-based life, phenotypic expression, adaptive mutagenesis, and functional maintenance of nondividing cells...
- Determinants of bistability in induction of the Escherichia coli lac operonD W Dreisigmeyer
Los Alamos National Laboratory, Theoretical Division, Los Alamos, NM, USA
IET Syst Biol 2:293-303. 2008The authors have developed a mathematical model of regulation of expression of the Escherichia coli lac operon, and have investigated bistability in its steady-state induction behaviour in the absence of external glucose...
- Fate mapping Nkx2.1-lineage cells in the mouse telencephalonQing Xu
Department of Psychiatry, Weill Medical College of Cornell University, New York, New York 10021, USA
J Comp Neurol 506:16-29. 2008..1-expressing regions. In this paper, we present fate-mapping data on Nkx2.1-lineage neurons throughout the telencephalon, including the cerebral cortex, amygdala, olfactory bulb, striatum, globus pallidus, septum, and nucleus basalis...
- Probing transcription factor dynamics at the single-molecule level in a living cellJohan Elf
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Science 316:1191-4. 2007..The methods and findings can be generalized to other nucleic acid binding proteins...
- Origin of bistability in the lac OperonM Santillan
Unidad Monterrey, Centro de Investigacion y Estudios Avanzados del Instituto Politecnico Nacional, Monterrey, Mexico
Biophys J 92:3830-42. 2007..In this work, we investigate the origin of bistability in the lac operon. To do this, we develop a mathematical model for the regulatory pathway in this system and compare the model ..
- The diffusive influx and carrier efflux have a strong effect on the bistability of the lac operon in Escherichia coliJason T Noel
Department of Chemical Engineering, University of Florida, Gainesville, FL 32611 6005, USA
J Theor Biol 256:14-28. 2009In the presence of gratuitous inducers, the lac operon of Escherichia coli exhibits bistability. Most models in the literature assume that the inducer enters the cell via the carrier (permease), and exits by a diffusion-like process...
- Single mRNA molecules demonstrate probabilistic movement in living mammalian cellsDahlene Fusco
Departments of Anatomy, Albert Einstein College of Medicine, 10461, Bronx, NY, USA
Curr Biol 13:161-7. 2003..Individual mRNA molecules switched frequently among these movements, suggesting that mRNAs undergo continuous cycles of anchoring, diffusion, and active transport...
- Sizing the holin lesion with an endolysin-beta-galactosidase fusionIng Nang Wang
Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843 2128, USA
J Bacteriol 185:779-87. 2003..A model for the formation of the membrane lesion within homo-oligomeric rafts of holin proteins is discussed...
- The lac operon of Lactobacillus casei contains lacT, a gene coding for a protein of the Bg1G family of transcriptional antiterminatorsC A Alpert
Fachbereich Biologie chemie, AG Genetik, Universitat Osnabruck, Germany
J Bacteriol 179:1555-62. 1997The 5' region of the lac operon of Lactobacillus casei has been investigated. An open reading frame of 293 codons, designated lacT, was identified upstream of lacE...
- Effects of Lac operon activation, deletion of the Yhha gene, and the removal of oxygen on the ultra-weak photon emission of Escherichia coliFrédéric M Laager
Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, Seoul, Korea
Electromagn Biol Med 28:240-9. 2009..After 30 min, it was observed that the emission levels equaled the emission levels of the sterile medium. We could therefore conclude that the source of the photon emission would be affected by genetic activity and is oxygen related...
- Effects of nucleoid proteins on DNA repression loop formation in Escherichia coliNicole A Becker
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First St SW, Rochester, MN 55905, USA
Nucleic Acids Res 35:3988-4000. 2007..One classic paradigm is DNA looping in the regulation of the Escherichia coli lac operon. Lac repressor protein binds simultaneously to two operator sequences flanking the lac promoter...
- A model system for analyzing somatic mutations in Drosophila melanogasterAna Maria Garcia
University of Texas at San Antonio, San Antonio, Texas 78249, USA
Nat Methods 4:401-3. 2007..The results obtained with the new model indicate two-to threefold higher frequencies of spontaneous mutations than in the mouse, with most of the mutations characterized as large genome rearrangements...
- Genomic instability in mouse Burkitt lymphoma is dominated by illegitimate genetic recombinations, not point mutationsLynne D Rockwood
Laboratory of Genetics, Center for Cancer Research CCR, NCI, Bethesda, Maryland, MD 20892, USA
Oncogene 21:7235-40. 2002..The results further suggest that a phenotype of hypermutability (elevated mutant frequency) may not always be required for oncogenesis to occur...
- Mechanism of regulation of transcription initiation by ppGpp. I. Effects of ppGpp on transcription initiation in vivo and in vitroM M Barker
Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI, 53706, USA
J Mol Biol 305:673-88. 2001..The accompanying paper evaluates potential models for positive control of amino acid promoters by ppGpp that might explain the requirement of ppGpp for amino acid prototrophy...
- Reduced differentiation potential of primary MyoD-/- myogenic cells derived from adult skeletal muscleL A Sabourin
Institute for Molecular Biology and Biotechnology, McMaster University, Hamilton, Ontario, Canada L8S 4K1
J Cell Biol 144:631-43. 1999..We interpret these data to suggest that MyoD-/- myogenic cells represent an intermediate stage between a quiescent satellite cell and a myogenic precursor cell...
- Loss of the bloom syndrome helicase increases DNA ligase 4-independent genome rearrangements and tumorigenesis in aging DrosophilaAna Maria Garcia
Department of Biology, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA
Genome Biol 12:R121. 2011..To address this, we used a lacZ reporter system in wild-type and several mutant strains of Drosophila melanogaster to analyze mechanisms of mutagenesis throughout their lifespan...
- Detection and analysis of somatic mutations at a lacZ reporter locus in higher organisms: application to Mus musculus and Drosophila melanogasterAna Maria Garcia
University of Texas at San Antonio, San Antonio, TX, USA
Methods Mol Biol 371:267-87. 2007..This system is capable of detecting a broad range of mutational events, varying from small mutations in the lacZ reporter gene to large genome rearrangements with one breakpoint in lacZ and the other breakpoint elsewhere in the genome...
- Comparison between in vivo mutagenicity and carcinogenicity in multiple organs by benzo[a]pyrene in the lacZ transgenic mouse (Muta Mouse)A Hakura
Drug Safety Research Laboratories, Ibaraki, Japan
Mutat Res 398:123-30. 1998..They also indicate, however, that the magnitude of the in vivo lacZ mutant frequencies induced by BP in different organs did not fully correlate with the target organs for carcinogenicity...
- Influence of catabolite repression and inducer exclusion on the bistable behavior of the lac operonMoises Santillan
Centre for Nonlinear Dynamics, and Departments of Physiology, Physics, and Mathematics, McGill University, H3G 1Y6 Montreal, Quebec, Canada
Biophys J 86:1282-92. 2004A mathematical model of the lac operon which includes all of the known regulatory mechanisms, including external-glucose-dependent catabolite repression and inducer exclusion, as well as the time delays inherent to transcription and ..
- Combinatorial transcriptional control of the lactose operon of Escherichia coliThomas Kuhlman
Center for Theoretical Biological Physics, University of California at San Diego, La Jolla, CA 92093 0374, USA
Proc Natl Acad Sci U S A 104:6043-8. 2007..Specifically, our analysis indicates that the sensitivity of the inducer response results from LacR-mediated DNA looping, which is significantly enhanced by CRP...
- lac operon induction in Escherichia coli: Systematic comparison of IPTG and TMG induction and influence of the transacetylase LacAAnja Marbach
Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr 1, 39106 Magdeburg, Germany
J Biotechnol 157:82-8. 2012Most commonly used expression systems in bacteria are based on the Escherichia coli lac promoter. Furthermore, lac operon elements are used today in systems and synthetic biology...
- Effects of different fixatives on beta-galactosidase activityMasafumi Takahashi
J Histochem Cytochem 51:553-4. 2003
- Embedding of bone samples in methylmethacrylate: a suitable method for tracking LacZ mesenchymal stem cells in skeletal tissuesD Hannouche
Laboratoire de Recherches Orthopediques, CNRS, Faculte de Medecine Lariboisiere Saint Louis, Universite Paris 7, 75010 Paris, France
J Histochem Cytochem 55:255-62. 2007..LacZ is a suitable phenotypic marker to track MSCs in skeletal tissues embedded in MMA...
- Corneal development, limbal stem cell function, and corneal epithelial cell migration in the Pax6(+/-) mouseJ Martin Collinson
Department of Biomedical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, Scotland, United Kingdom
Invest Ophthalmol Vis Sci 45:1101-8. 2004..To investigate the etiology of corneal dysfunction in the Pax6(+/-) mouse model of aniridia-related keratopathy...
- Mechanism of CRP-cAMP activation of lac operon transcription initiation activation of the P1 promoterT P Malan
J Mol Biol 180:881-909. 1984..DNA supercoiling enhanced the promoter strength of the lac wild-type and UV5 promoters. The combination of supercoiling and CRP-cAMP was necessary for optimal promoter strength for the lac wild-type promoter...
- Syn, anti, and finally both conformations of cyclic AMP are involved in the CRP-dependent transcription initiation mechanism in E. coli lac operonYusuf Tutar
Department of Chemistry, Cumhuriyet University, Sivas, Turkey
Cell Biochem Funct 26:399-405. 2008..Therefore, the aim of this review is to summarize recent works and developments on the cAMP-dependent CRP activation mechanism in E. coli...
- Allostery and the lac OperonMitchell Lewis
Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, 37th and Hamilton Walk, Philadelphia, PA 19104, USA
J Mol Biol 425:2309-16. 2013..Over the past half-century, the details of the lac operon and the allosteric model have been tested using genetic, biochemical, and structural techniques...
- Mechanism of transcriptional repression at a bacterial promoter by analysis of single moleculesAlvaro Sanchez
Graduate Program in Biophysics and Structural Biology, Brandeis University, Waltham, MA, USA
EMBO J 30:3940-6. 2011..The single-molecule statistical partitioning approach is broadly applicable to elucidating mechanisms of regulatory systems including those that are kinetically rather than thermodynamically controlled...
- Adaptive amplification: an inducible chromosomal instability mechanismP J Hastings
Department of Molecular and Human Genetics Baylor College of Medicine One Baylor Plaza Houston, TX 77030, USA
Cell 103:723-31. 2000..We report the discovery of adaptive amplification at the lac operon in Escherichia coli...
- In vivo imaging of beta-galactosidase activity using far red fluorescent switchChing Hsuan Tung
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
Cancer Res 64:1579-83. 2004..The results indicated that in vivo real-time detection of beta-gal activity is possible by fluorescence imaging technology...
- Structure of the lac operon galactoside acetyltransferaseXing Guo Wang
Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
Structure 10:581-8. 2002..3.1.18) is a gene product of the classical lac operon. GAT may assist cellular detoxification by acetylating nonmetabolizable pyranosides, thereby preventing their ..
- The tyrosinase related protein-1 (Tyrp1) promoter in transgenic experiments: targeted expression to the retinal pigment epitheliumF Beermann
ISREC Swiss Institute for Experimental Cancer Research, Epalinges
Cell Mol Biol (Noisy-le-grand) 45:961-8. 1999..Tyrp1-Tag transgenic mice developed malignant eye tumors of RPE origin, which invaded the optic nerve and led to metastasis into lymph nodes and spleen...
- Quantitative approaches to the study of bistability in the lac operon of Escherichia coliMoises Santillan
Centro de Investigacion y Estudios Avanzados del IPN, Unidad Monterrey, Parque de Investigación e Innovación Tecnológica, Apodaca, NL, Mexico
J R Soc Interface 5:S29-39. 2008In this paper, the history and importance of the lac operon in the development of molecular and systems biology are briefly reviewed...
- Kinetics of induction of DNA damage and lacZ gene mutations in stomach mucosa of mice treated with beta-propiolactone and N-methyl-N'-nitro-N-nitrosoguanidine, using single-cell gel electrophoresis and MutaMouse modelsD Brault
Rhone Poulenc Rorer, Nonclinical Safety Assessment, 13 quai Jules Guesde, BP 14, F 94403 Vitry sur Seine, France
Environ Mol Mutagen 34:182-9. 1999..These results illustrate the ability and complementarity of the SCGE and MutaMouse models to assess the genotoxicity of direct alkylating agents in the mouse gastric mucosa in vivo...
- Stochastic and deterministic simulations of heterogeneous cell population dynamicsNikos V Mantzaris
Department of Chemical and Biomolecular Engineering and Bioengineering Department, Rice University, Houston, TX 77005, USA
J Theor Biol 241:690-706. 2006..kinetics and (b) the expression dynamics of a genetic network with positive feedback architecture, such as the lac operon. The effects of stochastic division as well as those of different division and partitioning mechanisms were ..
- Glucose lowers CRP* levels resulting in repression of the lac operon in cells lacking cAMPH Tagami
Department of Molecular Biology, School of Science, Nagoya University, Japan
Mol Microbiol 17:251-8. 1995....
- Anionic liposomes increase the efficiency of adenovirus-mediated gene transfer to coxsackie-adenovirus receptor deficient cellsZhirong Zhong
Key Laboratory of Drug Targeting and Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, P R China
Mol Pharm 7:105-15. 2010..In conclusion, anionic liposomes prepared by the calcium-induced phase change method could significantly enhance the transduction ability of Ad5 in CAR deficient cells...
- Oxygen, nitrate, and molybdenum regulation of dmsABC gene expression in Escherichia coliP A Cotter
Department of Microbiology, University of California, Los Angeles
J Bacteriol 171:3817-23. 1989..Additionally, expression of dmsABC varied depending on the carbon source. Expression was highest when cells were grown on sorbitol...
- Dynamics and bistability in a reduced model of the lac operonNecmettin Yildirim
Centre for Nonlinear Dynamics, McGill University, Montreal, Quebec H3G 1Y6, Canada
Chaos 14:279-92. 2004It is known that the lac operon regulatory pathway is capable of showing bistable behavior...
- Lac repressor hinge flexibility and DNA looping: single molecule kinetics by tethered particle motionFrancesco Vanzi
LENS European Laboratory for Nonlinear Spectroscopy, University of Florence, Italy
Nucleic Acids Res 34:3409-20. 2006....
- Regulation of chondrocyte differentiation by Cbfa1I S Kim
Universitäts Kinderklinik Mainz, Langenbeckstrasse 1, 55101, Mainz, Germany
Mech Dev 80:159-70. 1999..Our studies identify Cbfa1 as a major positive regulator of chondrocyte differentiation...
- Increased mutant frequency by carbon black, but not quartz, in the lacZ and cII transgenes of muta mouse lung epithelial cellsNicklas Raun Jacobsen
National Research Centre for the Working Environment, Lersø Parkallé 105, DK 2100 Copenhagen Ø, Denmark
Environ Mol Mutagen 48:451-61. 2007..More studies are essential for understanding the biological significance of these findings, and clearly documenting DNA sequence changes. The results do not necessarily imply that other carbonaceous nano materials are genotoxic...
- Surveying a supercoil domain by using the gamma delta resolution system in Salmonella typhimuriumN P Higgins
Department of Biochemistry, University of Alabama at Birmingham, 35294 2170, USA
J Bacteriol 178:2825-35. 1996..10(-0.02x). We found that res sites can be plectonemically interwound over long distances ( > 100 kb) and that barriers to supercoil diffusion are placed stochastically within the 43- to 45-min region of the chromosome...
- A non-secreted form of IGF-1 is a protective factor for neural stem cellsANTHONY JOHN WINDEBANK; Fiscal Year: 2012..We will take advantage of an inducible gene expression system that utilizes control elements from the lac operon of E. coli to induce or repress transgene expression in the mouse...
- Structures of DNA and RNA Polymerases and Functional ComplexesTHOMAS ARTHUR STEITZ; Fiscal Year: 2013..coli will be examined from its structures complexed with the gal and lac operon promoter DNAs and the catabolite gene activator protein regulator transcription factor...
- Genetic circuits based on allosteric ribozymesAndrew D Ellington; Fiscal Year: 2010..are instrumental in modulating the expression of operons involved in the utilization of sugars, such as the lac operon. Starting with three different ribozyme platforms (hammerhead, L1 ligase, and Group I self-splicing intron) we ..
- MUTAGENIC AND RECOMBINOGENIC EFFECTS OF GAMMA RAYSWilliam Taylor; Fiscal Year: 1990..Mutagenesis will be measured as forward mutations of the Lac gene in plasmid PBRPI which contains the E. coli Lac operon cloned into PBR322. The host cells in this case will be delta lac...
- ANTHRACYCLINE-INDUCED DNA SEQUENCE SPECIFIC MUTATIONW Sedwick; Fiscal Year: 1993..proposal is to investigate the basis of the mutagenic specificity of anthracyclines for the lacO region of the lac operon, in vitro and in procaryotic and eukaryotic systems...
- PRIMARY STRUCTURE OF THE CYCLIC AMP RECEPTOR PROTEINDavid Schlesinger; Fiscal Year: 1980..an allosteric protein which exerts a positive control on polycistronic mRNA protein synthesis of enzymes of the lac operon in response to intracellular levels of cAMP...
- Non-antiobiotic resistance markers for bacteriaHerbert Schweizer; Fiscal Year: 2006..cassettes will be constructed by placing the respective genes under the control of modified Escherichia coli lac operon promoters...
- CYANASE AND THE CYANASE OPERONPaul Anderson; Fiscal Year: 1993..a permease for cyanate, cyanase, and a gene product of unknown function; the operon partially overlaps the lac operon, has been sequenced, and is controlled by a regulatory gene located only 108 base pairs upstream from the coding ..
- Transcription coupled DNA repair in E. ColiPhilip Hanawalt; Fiscal Year: 2006..III. We will measure DNA turnover in the undamaged lac operon when it is expressed, or repressed, comparing the frequency of "gratuitous" repair synthesis in each strand ..
- Mechanics of Transcriptional Control through DNA LoopingJens Christian Meiners; Fiscal Year: 2006..For this aim, a simplified model system for DNA looping based on the lac repressor and the lac operon in E. coli will be studied in vitro in a series of single-molecule experiments...
- LAC REPRESSOR MOUSEHeidi Scrable; Fiscal Year: 2004..the research proposed in this application is control of the endogenous mouse genome by elements derived from the lac operon of E. coli...
- SPECTROSCOPIC APPROACHES TO GENE REGULATIONPonzy Lu; Fiscal Year: 1990..We will approach this using three systems: a) The operator of the lac operon; b) the two sets of lambda phage operators OR and OL...
- CONTROLLED REGULATION OF SV40 T AG SYNTHESISJAMES DECAPRIO; Fiscal Year: 1992..Coli lac operon function efficiently in animal cells...
- MECHANISM AND REGULATION OF E. COLI RNA POLYMERASEWILLIAM MCCLURE; Fiscal Year: 1980..The mechanism of transcription activation will be investigated on the lac operon. We also want to know how ribosomal RNA and tRNA synthesis is controlled in E. coli...
- POSITIVE REGULATION OF METHIONINE BIOSYNTHESISGEORGE STAUFFER; Fiscal Year: 1992..The metE, metH and metR control regions have been fused to the lac operon. The metE-lac and metH-lac fusions will be used to isolate mutants with altered regulation by the positive ..
- BIOSYNTHESIS OF VITAMIN B12 AND ANAEROBIC METABOLISMJohn Roth; Fiscal Year: 2004..coli. Understanding this metabolism may contribute to understanding the natural lifestyle of Salmonellae. ..
- Molecular mechanism of DNA-polymerase choice in vivoSusan Rosenberg; Fiscal Year: 2007..coli DNA polymerases, and functions in DNA polymerase choice in vivo: a critical regulatory decision for cell survival of environmental damage, and genomic health. [unreadable] [unreadable] [unreadable]..
- DNA REPAIR IN DRUG RESISTANCE MUTATIONSusan Rosenberg; Fiscal Year: 2003....
- GENETIC CONTROL OF NITRATE RESPIRATION IN E. COLIValley J Stewart; Fiscal Year: 2010..Thus, results from the model species E. coli enhance understanding for a broad range of pathogens that significantly impact public health. ..
- Mutagenicity of tobacco smoke in human cell co-culturesJoseph Guttenplan; Fiscal Year: 2007..Clearly, it is preferable to determine their potential risk prospectively, rather than retrospectively. [unreadable] [unreadable] [unreadable]..
- Origins of Spontaneous MutagenesisJoseph Guttenplan; Fiscal Year: 2005..abstract_text> ..
- Gene therapy for treatment of craniofacial dysplasiaStephanos Kyrkanides; Fiscal Year: 2004..Completion of this study will aid in the development of novel therapies for the management of genetic disorders. ..
- CRANIOFACIAL DEVELOPMENTGURKIRPAL SOHAL; Fiscal Year: 2001..Results of this study likely will alter our interpretations of mechanisms of normal development, and of the underlying causes of craniofacial anomalies, which make up about 25 percent of all malformations. ..
- Joint degeneration: Somatic mosaic analysis in a transgenic mouseStephanos Kyrkanides; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Large-scale chromatin structure and dynamicsAndrew Belmont; Fiscal Year: 2007..Knowledge gained from this research should provide valuable insight into regulation of basic molecular processes and may serve to guide design of future gene therapy vectors and stable transgene expression. ..
- Contributions of VENT cells to the GI tractGURKIRPAL SOHAL; Fiscal Year: 2007..RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page .................................................................................................................................................. 1 Description, ..
- Does peripheral localized chronic inflammation predispose to neurodegeneration?Stephanos Kyrkanides; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Recombinant FIV vectors for the delivery of siRNA therapy to jointsStephanos Kyrkanides; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Mechanisms of Adaptive AmplificationPhilip J Hastings; Fiscal Year: 2010....
- NEURONAL FUNCTION IN CRANIOFACIAL DEVELOPMENTStephanos Kyrkanides; Fiscal Year: 2004..abstract_text> ..
- Morphogenesis of the inner earGURKIRPAL SOHAL; Fiscal Year: 2003..abstract_text> ..
- REGULATION BY THE ESCHERICHIA COLI SMALL RNA, DSRADARREN SLEDJESKI; Fiscal Year: 2002..3) To compare the effect of DsrA and H-NS on DNA structure in vivo. In vivo footprinting of multicopy plasmid genes regulated by H-NS/DsrA will be used to compare the effects of hns-mutations and overexpression of DsrA on DNA structure. ..
- Hix in Enteric Mesenchymal and Neuronal DevelopmentMichael Bates; Fiscal Year: 2005..abstract_text> ..
- Transcriptional Control of Early Coronary Vascular DevelopmentRAVINDRA P MISRA; Fiscal Year: 2010....
- Functional Genetic Tests for Breast Cancer DiagnosisGRANT BITTER; Fiscal Year: 2003..abstract_text> ..
- NEUTROPHIL RECRUITMENT IN LPS-INDUCED AIRWAY DISEASEJessica Moreland; Fiscal Year: 2004....
- Genome Evolution in Pathogens: The roles of predator-prey interactionsJeffrey G Lawrence; Fiscal Year: 2010....
- Tandemly Repetitive Proteins in MycoplasmasKEVIN F DYBVIG; Fiscal Year: 2010..By understanding the role of repetitive proteins in the disease process, it is anticipated that improved measures to control the infections of these significant human pathogens will be developed. ..
- Transcriptional Integration of MetabolismELTON YOUNG; Fiscal Year: 2007..abstract_text> ..
- Pulsed magnetic fields, neurons, and blood vesselsDiana Casper; Fiscal Year: 2006..Results could have applications not only in therapeutic strategies for Parkinson's disease, but in the treatment of other chronic and acute vascular and neurodegenerative diseases. ..
- ROLES OF BMP4 AND ITS REGULATION IN TOOTH DEVELOPMENTJian Feng; Fiscal Year: 2004..Molecular techniques include RT-PCR, in situ hybridization, DNA sequence determination, SSCP (single strand conformational polymorphism), and heteroduplex analysis. ..
- SELENIUM-INDUCED GLAUCOMAW Stamer; Fiscal Year: 2006..abstract_text> ..
- Antigen Identification in Occult ChorioretinopathiesJeffrey Bennett; Fiscal Year: 2006....
- CONTROL OF CELL GROWTHJonathan Gallant; Fiscal Year: 2005..Because starvation conditions and the occurrence of a hungry codon may be frequent in nature, these phenomena are important to an understanding of translation and its control. ..
- Molecular Basis of Myocilin Function in the Human EyeW Daniel Stamer; Fiscal Year: 2010..abstract_text> ..
- Gene Expression Patterns and Lifestyles in LegionellaHoward Shuman; Fiscal Year: 2009..abstract_text> ..
- Transplantation of Reduced-Size Fatty LiversZhi Zhong; Fiscal Year: 2010....
- CADHERIN DYNAMICS AND GLAUCOMAW Daniel Stamer; Fiscal Year: 2010..Results obtained from these investigations will provide a basic understanding of the role of cadherin proteins in aqueous outflow resistance and uncover novel therapeutic targets for glaucoma therapy. ..
- ASM Conference on Candida and CandidiasisAaron Mitchell; Fiscal Year: 2008..The conference will help chart the course of future research and facilitate the urgent necessity to understand, treat, and prevent disease. [unreadable] [unreadable] [unreadable]..
- K12 Expression: Cornea-Type Epithelial DifferentiationWINSTON KAO; Fiscal Year: 2008..Aim 3 is to examine roles of Fos on corneal-type epithelium differentiation and corneal wound healing as described in Aim 2. ..
- Coordinated control of a regulatory network by gcvB RNAGEORGE STAUFFER; Fiscal Year: 2008..Second, we will determine the scope of the regulatory network and the molecular mechanisms of control by the GcvB RNAs. [unreadable] [unreadable]..
- Endothelial Nitric Oxide Synthase & Portal HypertensionVijay Shah; Fiscal Year: 2002..abstract_text> ..
- RECOMBINOGENIC ENGINEERING OF PATHOGENIC BACTERIAKENAN MURPHY; Fiscal Year: 2003....
- GENETIC RECOMBINATION IN MYCOPLASMASKevin Dybvig; Fiscal Year: 2004..In aim 2, mutants with defects in putative DNA recombinases and V-I protein production will be examined to study the mechanisms of gene rearrangements and phenotypic switching. ..
- CADHERIN REGULATION OF AQUEOUS HUMOR OUTFLOWW Stamer; Fiscal Year: 2004..abstract_text> ..
- Fucosylated cell surface molecules in tumor invasionSTEVEN DOMINO; Fiscal Year: 2005..and in 1,2-dimethylhydrazine-induced tumors, in the presence and absence of cell surface alpha(1,2)fucose by crossing mice into the Fut1 and Fut2 null background. ..
- Regulation and function of Pseudomonas drug efflux pumpsHerbert Schweizer; Fiscal Year: 2006..Aim 3: Probe other, perhaps global regulators of efflux operon expression, specifically MexS and PA4878. ..
- Imageable tumor-targeting bacteriaMing Zhao; Fiscal Year: 2006..unreadable] [unreadable] [unreadable]..
- Methionine Recycling Pathways in KlebsiellaJeffrey Lawrence; Fiscal Year: 2006..In this way, characterization of these pathways sheds light on the evolution of complex metabolism, and on the selective forces that lead to gene acquisition, gene loss, and genome change. ..
- REGULATION OF E COLI CYTOCHROME OXIDASE AND TCA CYCLERobert Gunsalus; Fiscal Year: 2001..coli. These studies should provide a detailed understanding of how the cell coordinates pathways for electron and carbon flow within the cell during transition between oxygen rich and oxygen limiting conditions. ..