lac operon

Summary

Summary: The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.

Top Publications

  1. ncbi Crystal structure of the lactose operon repressor and its complexes with DNA and inducer
    M Lewis
    Johnson Research Foundation, University of Pennsylvania, Philadelphia 19104, USA
    Science 271:1247-54. 1996
  2. ncbi Multistability in the lactose utilization network of Escherichia coli
    Ertugrul M Ozbudak
    Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 427:737-40. 2004
  3. ncbi Optimality and evolutionary tuning of the expression level of a protein
    Erez Dekel
    Department of Molecular Cell Biology and Department of Physics of Complex Systems, The Weizmann Institute of Science, Rehovot 76100, Israel
    Nature 436:588-92. 2005
  4. pmc The cost of expression of Escherichia coli lac operon proteins is in the process, not in the products
    Daniel M Stoebel
    Department of Ecology and Evolution, Stony Brook University, Stony Brook, New York 11794, USA
    Genetics 178:1653-60. 2008
  5. pmc Transcriptional infidelity promotes heritable phenotypic change in a bistable gene network
    Alasdair J E Gordon
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA
    PLoS Biol 7:e44. 2009
  6. pmc A stochastic single-molecule event triggers phenotype switching of a bacterial cell
    Paul J Choi
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 322:442-6. 2008
  7. pmc Interplay of protein and DNA structure revealed in simulations of the lac operon
    Luke Czapla
    Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America
    PLoS ONE 8:e56548. 2013
  8. pmc Comparison of deterministic and stochastic models of the lac operon genetic network
    Michail Stamatakis
    Department of Chemical and Biomolecular Engineering, Rice University, Houston, Texas 77005, USA
    Biophys J 96:887-906. 2009
  9. pmc Lysophosphatidylcholine as an adjuvant for lentiviral vector mediated gene transfer to airway epithelium: effect of acyl chain length
    Patricia Cmielewski
    Dept of Respiratory and Sleep Medicine, Women s and Children s Hospital, 72 King William Rd, North Adelaide, SA 5006, Australia
    Respir Res 11:84. 2010
  10. pmc Analysis of in-vivo LacR-mediated gene repression based on the mechanics of DNA looping
    Yongli Zhang
    Departments of Chemistry and Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, United States of America
    PLoS ONE 1:e136. 2006

Research Grants

  1. A non-secreted form of IGF-1 is a protective factor for neural stem cells
    ANTHONY JOHN WINDEBANK; Fiscal Year: 2012
  2. Structures of DNA and RNA Polymerases and Functional Complexes
    THOMAS ARTHUR STEITZ; Fiscal Year: 2013
  3. Genetic circuits based on allosteric ribozymes
    Andrew D Ellington; Fiscal Year: 2010
  4. MUTAGENIC AND RECOMBINOGENIC EFFECTS OF GAMMA RAYS
    William Taylor; Fiscal Year: 1990
  5. ANTHRACYCLINE-INDUCED DNA SEQUENCE SPECIFIC MUTATION
    W Sedwick; Fiscal Year: 1993
  6. PRIMARY STRUCTURE OF THE CYCLIC AMP RECEPTOR PROTEIN
    David Schlesinger; Fiscal Year: 1980
  7. Non-antiobiotic resistance markers for bacteria
    Herbert Schweizer; Fiscal Year: 2006
  8. CYANASE AND THE CYANASE OPERON
    Paul Anderson; Fiscal Year: 1993
  9. Transcription coupled DNA repair in E. Coli
    Philip Hanawalt; Fiscal Year: 2006
  10. Mechanics of Transcriptional Control through DNA Looping
    Jens Christian Meiners; Fiscal Year: 2006

Detail Information

Publications267 found, 100 shown here

  1. ncbi Crystal structure of the lactose operon repressor and its complexes with DNA and inducer
    M Lewis
    Johnson Research Foundation, University of Pennsylvania, Philadelphia 19104, USA
    Science 271:1247-54. 1996
    The lac operon of Escherichia coli is the paradigm for gene regulation. Its key component is the lac repressor, a product of the lacI gene...
  2. ncbi Multistability in the lactose utilization network of Escherichia coli
    Ertugrul M Ozbudak
    Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 427:737-40. 2004
    ..The phase diagram thus serves as a sensitive probe of molecular interactions and as a powerful tool for rational network design...
  3. ncbi Optimality and evolutionary tuning of the expression level of a protein
    Erez Dekel
    Department of Molecular Cell Biology and Department of Physics of Complex Systems, The Weizmann Institute of Science, Rehovot 76100, Israel
    Nature 436:588-92. 2005
    ..Thus, protein expression from the lac operon seems to be a solution of a cost-benefit optimization problem, and can be rapidly tuned by evolution to function ..
  4. pmc The cost of expression of Escherichia coli lac operon proteins is in the process, not in the products
    Daniel M Stoebel
    Department of Ecology and Evolution, Stony Brook University, Stony Brook, New York 11794, USA
    Genetics 178:1653-60. 2008
    ..Our results identify the basis of a single selective pressure likely acting across the entire E. coli transcriptome...
  5. pmc Transcriptional infidelity promotes heritable phenotypic change in a bistable gene network
    Alasdair J E Gordon
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA
    PLoS Biol 7:e44. 2009
    ..We used a classic bistable memory module to monitor and capture transient RNA errors: the lac operon of Escherichia coli comprises an autocatalytic positive feedback loop producing a heritable all-or-none ..
  6. pmc A stochastic single-molecule event triggers phenotype switching of a bacterial cell
    Paul J Choi
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 322:442-6. 2008
    ..single-molecule sensitivity, we investigated the molecular mechanism of how an Escherichia coli cell with the lac operon switches from one phenotype to another...
  7. pmc Interplay of protein and DNA structure revealed in simulations of the lac operon
    Luke Czapla
    Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America
    PLoS ONE 8:e56548. 2013
    ..The short loops implicated in the regulation of the lac operon suggest the involvement of factors other than DNA and repressor in gene control...
  8. pmc Comparison of deterministic and stochastic models of the lac operon genetic network
    Michail Stamatakis
    Department of Chemical and Biomolecular Engineering, Rice University, Houston, Texas 77005, USA
    Biophys J 96:887-906. 2009
    The lac operon has been a paradigm for genetic regulation with positive feedback, and several modeling studies have described its dynamics at various levels of detail...
  9. pmc Lysophosphatidylcholine as an adjuvant for lentiviral vector mediated gene transfer to airway epithelium: effect of acyl chain length
    Patricia Cmielewski
    Dept of Respiratory and Sleep Medicine, Women s and Children s Hospital, 72 King William Rd, North Adelaide, SA 5006, Australia
    Respir Res 11:84. 2010
    ..We examined the electrophysiological and physical effect of airway pre-treatment with variants of LPC on lentiviral (LV) vector gene transfer efficiency in murine nasal airways in vivo...
  10. pmc Analysis of in-vivo LacR-mediated gene repression based on the mechanics of DNA looping
    Yongli Zhang
    Departments of Chemistry and Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, United States of America
    PLoS ONE 1:e136. 2006
    ....
  11. pmc Unequal fidelity of leading strand and lagging strand DNA replication on the Escherichia coli chromosome
    I J Fijalkowska
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02 106 Warsaw, Pawinskiego 5A, Poland
    Proc Natl Acad Sci U S A 95:10020-5. 1998
    ..We have constructed a series of lacZ strains in which the lac operon is inserted into the bacterial chromosome in the two possible orientations with regard to the chromosomal ..
  12. ncbi A switch from high-fidelity to error-prone DNA double-strand break repair underlies stress-induced mutation
    Rebecca G Ponder
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell 19:791-804. 2005
    ..Our findings reveal an RpoS-controlled switch from high-fidelity to mutagenic DSBR under stress. This limits genetic instability both in time and to localized genome regions, potentially important evolutionary strategies...
  13. pmc Isolation and characterization of RNA polymerase rpoB mutations that alter transcription slippage during elongation in Escherichia coli
    Yan Ning Zhou
    National Cancer Institute, National Institutes of Health, Frederick National Laboratory for Cancer Research, Frederick, Maryland 21702, USA
    J Biol Chem 288:2700-10. 2013
    ..Our E. coli study complements an accompanying study of slippage by yeast RNAP II and provides the basis for future studies on the mechanism of transcription fidelity...
  14. pmc Genetic analysis of prokaryotic and eukaryotic DNA-binding proteins in Escherichia coli
    F W Whipple
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Nucleic Acids Res 26:3700-6. 1998
    ..coli strains bearing reporters for other types of genetic systems, including repression-based and activation-based systems in which chimeric proteins are used to examine interactions between foreign protein domains...
  15. pmc Operator sequence alters gene expression independently of transcription factor occupancy in bacteria
    Hernan G Garcia
    Department of Physics, California Institute of Technology, Pasadena, CA 91125, USA
    Cell Rep 2:150-61. 2012
    ....
  16. ncbi SOS mutator DNA polymerase IV functions in adaptive mutation and not adaptive amplification
    G J McKenzie
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030 3411, USA
    Mol Cell 7:571-9. 2001
    ..coli lac operon. A nonpolar dinB mutation reduces adaptive mutation frequencies by 85% but does not affect adaptive ..
  17. ncbi Evidence for fusion between cardiac and skeletal muscle cells
    Hans Reinecke
    Department of Pathology, University of Washington, Box 357470, HSB Room D 514 Seattle, Wash 98195 7470, USA
    Circ Res 94:e56-60. 2004
    ..The full text of this article is available online at http://circres.ahajournals.org...
  18. ncbi Assaying promoter activity using LacZ and GFP as reporters
    Paul Carroll
    Institute of Cell and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, 4 Newark Street, Whitechapel, London E1 2AT, UK
    Methods Mol Biol 465:265-77. 2009
    ..This chapter describes two reporter systems (GFP and LacZ) used for determining promoter activity that have been widely used in mycobacteria...
  19. ncbi Bacterial repression loops require enhanced DNA flexibility
    Nicole A Becker
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    J Mol Biol 349:716-30. 2005
    The Escherichia coli lac operon provides a classic paradigm for understanding regulation of gene transcription...
  20. ncbi Development of new inbred transgenic strains of rats with LacZ or GFP
    Hirokazu Inoue
    Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, Tochigi 329 0498, Japan
    Biochem Biophys Res Commun 329:288-95. 2005
    ..Our Tg-rat system may provide great benefits for the elucidation of the cellular process of regenerative medicine, including cell and tissue transplantation...
  21. ncbi Cost-benefit theory and optimal design of gene regulation functions
    Tomer Kalisky
    Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
    Phys Biol 4:229-45. 2007
    ..We apply this theory to the well-studied lac operon of E. coli...
  22. pmc The mutY gene: a mutator locus in Escherichia coli that generates G.C----T.A transversions
    Y Nghiem
    Molecular Biology Institute, University of California, Los Angeles 90024
    Proc Natl Acad Sci U S A 85:2709-13. 1988
    ..coli. The mutY locus may act in a similar but reciprocal fashion to the previously characterized mutT locus, which results in A.T----C.G transversions...
  23. ncbi Interferon-gamma induced medulloblastoma in the developing cerebellum
    Wensheng Lin
    Jack Miller Center for Peripheral Neuropathy, Department of Neurology, University of Chicago, Chicago, Illinois 60637, USA
    J Neurosci 24:10074-83. 2004
    ..Thus, IFN-gamma expression in the perinatal period can induce SHH expression and medulloblastoma in the cerebellum by a STAT1-dependent mechanism, and its continued presence appears to promote a host response to the tumor...
  24. ncbi Bactericidal antisense effects of peptide-PNA conjugates
    L Good
    Center for Genomics Research, Karolinska Institute, Berzelius väg 37, 171 77, Stockholm, Sweden
    Nat Biotechnol 19:360-4. 2001
    ..These results indicate that peptides can be used to carry antisense PNA agents into bacteria. Such peptide-PNA conjugates open exciting possibilities for anti-infective drug development and provide new tools for microbial genetics...
  25. ncbi Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice
    F Shalaby
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Nature 376:62-6. 1995
    ..These results indicate that Flk-1 is essential for yolk-sac blood-island formation and vasculogenesis in the mouse embryo...
  26. pmc Pre-dispositions and epigenetic inheritance in the Escherichia coli lactose operon bistable switch
    Lydia Robert
    INSERM, and Faculty of Medicine, Paris Descartes University, 24 Rue de Faubourg St Jacques, Paris, France
    Mol Syst Biol 6:357. 2010
    ..Thus, stochasticity at the local level of the network and global physiology are synergistically involved in cell response determination...
  27. ncbi Activation of the receptor tyrosine kinase Kit is required for the proliferation of melanoblasts in the mouse embryo
    M A MacKenzie
    MRC Human Genetics Unit, Western General Hospital, Edinburgh, EH4 2XU, United Kingdom
    Dev Biol 192:99-107. 1997
    ..Our data do not describe a dependence upon Kit for melanoblast migration or differentiation...
  28. ncbi Matrix metalloproteinase-9 is required for adequate angiogenic revascularization of ischemic tissues: potential role in capillary branching
    Chad Johnson
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology Emory University School of Medicine, Atlanta, GA, USA
    Circ Res 94:262-8. 2004
    ..Our study indicates that angiogenesis triggered by tissue ischemia requires MMP-9, which may be involved in capillary branching, a potential novel role for this MMP that could be exploited to control angiogenesis...
  29. ncbi High local concentration: a fundamental strategy of life
    Stefan Oehler
    Biomedical Sciences Research Center Alexander Fleming, 34 Fleming Street, GR 16672 Vari, Greece
    J Mol Biol 395:242-53. 2010
    ..Local increase in concentration leads to cooperative or competitive interactions between molecules. It is an important principle of life...
  30. ncbi Development of genetic circuitry exhibiting toggle switch or oscillatory behavior in Escherichia coli
    Mariette R Atkinson
    Department of Biological Chemistry, University of Michigan School of Medicine, Ann Arbor, MI 48109, USA
    Cell 113:597-607. 2003
    ..For the toggle switch, LacI was provided constitutively; the level of active repressor was controlled by using a lacY mutant and varying the concentration of IPTG. This circuitry provided nearly discontinuous expression of activator...
  31. ncbi Novel Streptococcus infantarius subsp. infantarius variants harboring lactose metabolism genes homologous to Streptococcus thermophilus
    Christoph Jans
    Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland
    Food Microbiol 31:33-42. 2012
    ..7-100% sequence identity to S. infantarius subsp. infantarius ATCC BAA-102(T) and a gal-lac operon with 91.7-97.6% sequence identity to S. thermophilus, absent in all sequenced SBSEC strains analyzed...
  32. pmc In vivo tests of thermodynamic models of transcription repressor function
    Sudheer Tungtur
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS 66160, United States
    Biophys Chem 159:142-51. 2011
    ..Further analysis of each system suggests that the observed discrepancies between in vitro and in vivo results reflect the contributions of additional equilibria to the transcription repression process...
  33. ncbi Organ-specific increase in mutation accumulation and apoptosis rate in CuZn-superoxide dismutase-deficient mice
    Rita A Busuttil
    Departments of Physiology and Molecular Medicine, University of Texas Health Science Center, San Antonio, 78240, USA
    Cancer Res 65:11271-5. 2005
    ..5-fold was found not earlier than at 6 months. No increased mutation accumulation was observed in brain or spleen. These results support the hypothesis, that oxidative stress is an important causal factor of cancer in mammals...
  34. pmc The tandem inversion duplication in Salmonella enterica: selection drives unstable precursors to final mutation types
    Elisabeth Kugelberg
    Department of Microbiology, College of Biological Sciences, University of California, Davis, CA 95616, USA
    Genetics 185:65-80. 2010
    ..Growth improves with each step in formation of an SJ or aTID amplification, allowing selection to favor completion of the mutation process...
  35. ncbi The outflow tract of the heart is recruited from a novel heart-forming field
    C H Mjaatvedt
    Department of Cell Biology and Anatomy, Medical University of South Carolina, 171 Ashley Avenue, Charleston, South Carolina 29425, USA
    Dev Biol 238:97-109. 2001
    ..This new concept of the heart outlet's embryonic origin provides a new basis for explaining a variety of gene-expression patterns and cardiac defects described in both transgenic animals and human congenital heart disease...
  36. pmc Interconvertible lac repressor-DNA loops revealed by single-molecule experiments
    Oi Kwan Wong
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts, USA
    PLoS Biol 6:e232. 2008
    ..The ability to switch between different loop structures may help to explain how robust transcription regulation is maintained even though the mechanical work required to form a loop may change substantially with metabolic conditions...
  37. pmc Mutability and importance of a hypermutable cell subpopulation that produces stress-induced mutants in Escherichia coli
    Caleb Gonzalez
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS Genet 4:e1000208. 2008
    ..e., it is important. We consider a model in which HMS differentiation is controlled by stress responses. Differentiation of an HMS potentially limits the risks of mutagenesis in cell clones...
  38. ncbi Multiple developmental roles of VEGF suggested by a LacZ-tagged allele
    L Miquerol
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G1X5, Canada
    Dev Biol 212:307-22. 1999
    ..We believe that the LacZ-tagged allele we have generated offers a precise means of detecting VEGF expression under a variety of physiological and pathological conditions...
  39. ncbi Efficient Cre-mediated deletion in cardiac progenitor cells conferred by a 3'UTR-ires-Cre allele of the homeobox gene Nkx2-5
    Edouard G Stanley
    Victor Chang Cardiac Research Institute, St Vincent s Hospital, Darlinghurst, NSW, Australia
    Int J Dev Biol 46:431-9. 2002
    ..Our data suggest that the Nkx2-5IRESCre strain will be useful for genetic dissection of the multiple tiers of lineage allocation to the forming heart as well as of molecular interactions within the heart fields and heart tube...
  40. ncbi Gcm2 and Foxn1 mark early parathyroid- and thymus-specific domains in the developing third pharyngeal pouch
    J Gordon
    Centre for Genome Research Institute of Cell, Animal and Population Biology, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
    Mech Dev 103:141-3. 2001
    ..25. Our results show that Gcm2 and Foxn1 expression mark two complementary domains that prefigure parathyroid and thymus regions within the common primordium before morphological distinctions are present...
  41. pmc A focused and efficient genetic screening strategy in the mouse: identification of mutations that disrupt cortical development
    Konstantinos Zarbalis
    Department of Neurology and the Ernest Gallo Clinic and Research Center, University of California at San Francisco, Emeryville, USA
    PLoS Biol 2:E219. 2004
    ..Our results also highlight the utility of focused screens in the mouse, in addition to the large-scale and broadly targeted screens that are being carried out at mutagenesis centers...
  42. pmc Optimality and evolution of transcriptionally regulated gene expression
    Frank J Poelwijk
    AMOLF Institute, Science Park 104, 1098 XG, Amsterdam, The Netherlands
    BMC Syst Biol 5:128. 2011
    ..The cost and benefit of gene expression are the driving factors. While the former can be determined by gratuitous induction, the latter is difficult to measure directly...
  43. pmc The effect of stochasticity on the lac operon: an evolutionary perspective
    Milan van Hoek
    Theoretical Biology Bioinformatics Group, Utrecht University, Utrecht, The Netherlands
    PLoS Comput Biol 3:e111. 2007
    ..In some systems, noise in gene expression has been quantified, in among others the lac operon of Escherichia coli...
  44. ncbi Whole mount enzyme histochemistry as a rapid screen at necropsy for expression of beta-galactosidase (LacZ)-bearing transgenes: considerations for separating specific LacZ activity from nonspecific (endogenous) galactosidase activity
    Brad Bolon
    GEMpath Inc, Cedar City, Utah 84720 8400, USA
    Toxicol Pathol 36:265-76. 2008
    ....
  45. ncbi Boolean models can explain bistability in the lac operon
    Alan Veliz-Cuba
    Department of Mathematics, University of Nebraska Lincoln, Lincoln, Nebraska, USA
    J Comput Biol 18:783-94. 2011
    The lac operon in Escherichia coli has been studied extensively and is one of the earliest gene systems found to undergo both positive and negative control...
  46. ncbi The lac operon galactoside acetyltransferase
    Steven L Roderick
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    C R Biol 328:568-75. 2005
    Of the proteins encoded by the three structural genes of the lac operon, the galactoside acetyltransferase (thiogalactoside transacetylase, LacA, GAT) encoded by lacA is the only protein whose biological role remains in doubt...
  47. pmc Cooperation between translating ribosomes and RNA polymerase in transcription elongation
    Sergey Proshkin
    Department of Biochemistry, New York University School of Medicine, New York, NY 10016, USA
    Science 328:504-8. 2010
    ..Such a cooperative mechanism ensures that the transcriptional yield is always adjusted to translational needs at different genes and under various growth conditions...
  48. pmc Secrets of the lac operon. Glucose hysteresis as a mechanism in dietary restriction, aging and disease
    Charles V Mobbs
    Departments of Neuroscience and Geriatrics, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    Interdiscip Top Gerontol 35:39-68. 2007
    ..toxic effects of glucose are suggested by two general principles of metabolic processes, illustrated by the lac operon but also observed with glucose-induced gene expression...
  49. ncbi Stochastic switching in gene networks can occur by a single-molecule event or many molecular steps
    Paul J Choi
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    J Mol Biol 396:230-44. 2010
    ..Finally, we apply our methods to recent experimental data on switching in Escherichia coli lactose metabolism, providing quantitative interpretations of a single-molecule switching mechanism...
  50. ncbi Tyrosinase-related protein 2 promoter targets transgene expression to ocular and neural crest-derived tissues
    S Zhao
    Department of Cell Biology, Baylor College of Medicine, Houston, Texas, 77030, USA
    Dev Biol 216:154-63. 1999
    ..These studies demonstrate that the TRP2 promoter is valuable for transgenic studies of ocular differentiation and development of neural crest cells...
  51. ncbi Photoacoustic imaging of lacZ gene expression in vivo
    Li Li
    Washington University in St Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, St Louis, Missouri 63130, USA
    J Biomed Opt 12:020504. 2007
    ..With the future development of new absorption-based reporter gene systems, it is anticipated that photoacoustic technology will provide a valuable tool for molecular imaging research...
  52. pmc Adaptive mutation in Escherichia coli
    Patricia L Foster
    Department of Biology, Indiana University, Jordan Hall, 1001 East Third St, Bloomington, IN 47405, USA
    J Bacteriol 186:4846-52. 2004
  53. ncbi Myogenic determination occurs independently in somites and limb buds
    B Kablar
    Institute for Molecular Biology and Biotechnology, Cancer Research Group, McMaster University, Hamilton, Ontario, L8S 4K1, Canada
    Dev Biol 206:219-31. 1999
    ..Therefore, cells are recruited into the MyoD-dependent myogenic lineage through activation of the -20-kb MyoD enhancer and this occurs independently in somites and limb buds...
  54. ncbi Tbr1 regulates differentiation of the preplate and layer 6
    R F Hevner
    Nina Ireland Laboratory of, Developmental Neurobiology, Department of Psychiatry, University of California, San Francisco, San Francisco, CA 94143, USA
    Neuron 29:353-66. 2001
    ..These results show that Tbr1 is a common genetic determinant for the differentiation of early-born glutamatergic neocortical neurons and provide insights into the functions of these neurons as regulators of cortical development...
  55. ncbi The regulation of MyoD gene expression: conserved elements mediate expression in embryonic axial muscle
    A Asakura
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA
    Dev Biol 171:386-98. 1995
    ..The functional conservation of the regulatory region of the mouse myoD gene in Xenopus embryos suggests that the regulatory pathway that activates myoD gene transcription in axial muscles is conserved among vertebrates...
  56. pmc Enhancement of DNA flexibility in vitro and in vivo by HMGB box A proteins carrying box B residues
    Nadia T Sebastian
    Department of Chemistry, Creighton University, 2500 California Place, Omaha, Nebraska 68178, USA
    Biochemistry 48:2125-34. 2009
    ..These results demonstrate important roles for cationic leader amino acids in HMGB folding, DNA interaction, and DNA bending...
  57. ncbi The mouse Gtl2 gene is differentially expressed during embryonic development, encodes multiple alternatively spliced transcripts, and may act as an RNA
    K Schuster-Gossler
    Jackson Laboratory, Bar Harbor, Maine, USA
    Dev Dyn 212:214-28. 1998
    ..Gtl2 transcript levels are present in parthenogenic embryos but may be reduced, consistent with the pattern of inheritance of the Gtl2lacZ phenotype...
  58. pmc Demand theory of gene regulation. II. Quantitative application to the lactose and maltose operons of Escherichia coli
    M A Savageau
    Department of Microbiology and Immunology, The University of Michigan, Ann Arbor, Michigan 48109 0620, USA
    Genetics 149:1677-91. 1998
    ..Finally, the theory predicts an optimum value of C that is in agreement with the observed frequency for E. coli colonizing the human intestinal tract...
  59. ncbi Counteraction by MutT protein of transcriptional errors caused by oxidative damage
    F Taddei
    Institut Jacques Monod, Tour 43, 2 place Jussieu, 75251 Paris Cedex 05, France
    Science 278:128-30. 1997
    ..Such control of transcriptional fidelity by the ubiquitous MutT function has implications for evolution of RNA-based life, phenotypic expression, adaptive mutagenesis, and functional maintenance of nondividing cells...
  60. ncbi Determinants of bistability in induction of the Escherichia coli lac operon
    D W Dreisigmeyer
    Los Alamos National Laboratory, Theoretical Division, Los Alamos, NM, USA
    IET Syst Biol 2:293-303. 2008
    The authors have developed a mathematical model of regulation of expression of the Escherichia coli lac operon, and have investigated bistability in its steady-state induction behaviour in the absence of external glucose...
  61. ncbi Fate mapping Nkx2.1-lineage cells in the mouse telencephalon
    Qing Xu
    Department of Psychiatry, Weill Medical College of Cornell University, New York, New York 10021, USA
    J Comp Neurol 506:16-29. 2008
    ..1-expressing regions. In this paper, we present fate-mapping data on Nkx2.1-lineage neurons throughout the telencephalon, including the cerebral cortex, amygdala, olfactory bulb, striatum, globus pallidus, septum, and nucleus basalis...
  62. pmc Probing transcription factor dynamics at the single-molecule level in a living cell
    Johan Elf
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 316:1191-4. 2007
    ..The methods and findings can be generalized to other nucleic acid binding proteins...
  63. pmc Origin of bistability in the lac Operon
    M Santillan
    Unidad Monterrey, Centro de Investigacion y Estudios Avanzados del Instituto Politecnico Nacional, Monterrey, Mexico
    Biophys J 92:3830-42. 2007
    ..In this work, we investigate the origin of bistability in the lac operon. To do this, we develop a mathematical model for the regulatory pathway in this system and compare the model ..
  64. ncbi The diffusive influx and carrier efflux have a strong effect on the bistability of the lac operon in Escherichia coli
    Jason T Noel
    Department of Chemical Engineering, University of Florida, Gainesville, FL 32611 6005, USA
    J Theor Biol 256:14-28. 2009
    In the presence of gratuitous inducers, the lac operon of Escherichia coli exhibits bistability. Most models in the literature assume that the inducer enters the cell via the carrier (permease), and exits by a diffusion-like process...
  65. ncbi Single mRNA molecules demonstrate probabilistic movement in living mammalian cells
    Dahlene Fusco
    Departments of Anatomy, Albert Einstein College of Medicine, 10461, Bronx, NY, USA
    Curr Biol 13:161-7. 2003
    ..Individual mRNA molecules switched frequently among these movements, suggesting that mRNAs undergo continuous cycles of anchoring, diffusion, and active transport...
  66. pmc Sizing the holin lesion with an endolysin-beta-galactosidase fusion
    Ing Nang Wang
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843 2128, USA
    J Bacteriol 185:779-87. 2003
    ..A model for the formation of the membrane lesion within homo-oligomeric rafts of holin proteins is discussed...
  67. pmc The lac operon of Lactobacillus casei contains lacT, a gene coding for a protein of the Bg1G family of transcriptional antiterminators
    C A Alpert
    Fachbereich Biologie chemie, AG Genetik, Universitat Osnabruck, Germany
    J Bacteriol 179:1555-62. 1997
    The 5' region of the lac operon of Lactobacillus casei has been investigated. An open reading frame of 293 codons, designated lacT, was identified upstream of lacE...
  68. ncbi Effects of Lac operon activation, deletion of the Yhha gene, and the removal of oxygen on the ultra-weak photon emission of Escherichia coli
    Frédéric M Laager
    Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, Seoul, Korea
    Electromagn Biol Med 28:240-9. 2009
    ..After 30 min, it was observed that the emission levels equaled the emission levels of the sterile medium. We could therefore conclude that the source of the photon emission would be affected by genetic activity and is oxygen related...
  69. pmc Effects of nucleoid proteins on DNA repression loop formation in Escherichia coli
    Nicole A Becker
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First St SW, Rochester, MN 55905, USA
    Nucleic Acids Res 35:3988-4000. 2007
    ..One classic paradigm is DNA looping in the regulation of the Escherichia coli lac operon. Lac repressor protein binds simultaneously to two operator sequences flanking the lac promoter...
  70. pmc A model system for analyzing somatic mutations in Drosophila melanogaster
    Ana Maria Garcia
    University of Texas at San Antonio, San Antonio, Texas 78249, USA
    Nat Methods 4:401-3. 2007
    ..The results obtained with the new model indicate two-to threefold higher frequencies of spontaneous mutations than in the mouse, with most of the mutations characterized as large genome rearrangements...
  71. ncbi Genomic instability in mouse Burkitt lymphoma is dominated by illegitimate genetic recombinations, not point mutations
    Lynne D Rockwood
    Laboratory of Genetics, Center for Cancer Research CCR, NCI, Bethesda, Maryland, MD 20892, USA
    Oncogene 21:7235-40. 2002
    ..The results further suggest that a phenotype of hypermutability (elevated mutant frequency) may not always be required for oncogenesis to occur...
  72. ncbi Mechanism of regulation of transcription initiation by ppGpp. I. Effects of ppGpp on transcription initiation in vivo and in vitro
    M M Barker
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI, 53706, USA
    J Mol Biol 305:673-88. 2001
    ..The accompanying paper evaluates potential models for positive control of amino acid promoters by ppGpp that might explain the requirement of ppGpp for amino acid prototrophy...
  73. pmc Reduced differentiation potential of primary MyoD-/- myogenic cells derived from adult skeletal muscle
    L A Sabourin
    Institute for Molecular Biology and Biotechnology, McMaster University, Hamilton, Ontario, Canada L8S 4K1
    J Cell Biol 144:631-43. 1999
    ..We interpret these data to suggest that MyoD-/- myogenic cells represent an intermediate stage between a quiescent satellite cell and a myogenic precursor cell...
  74. pmc Loss of the bloom syndrome helicase increases DNA ligase 4-independent genome rearrangements and tumorigenesis in aging Drosophila
    Ana Maria Garcia
    Department of Biology, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA
    Genome Biol 12:R121. 2011
    ..To address this, we used a lacZ reporter system in wild-type and several mutant strains of Drosophila melanogaster to analyze mechanisms of mutagenesis throughout their lifespan...
  75. ncbi Detection and analysis of somatic mutations at a lacZ reporter locus in higher organisms: application to Mus musculus and Drosophila melanogaster
    Ana Maria Garcia
    University of Texas at San Antonio, San Antonio, TX, USA
    Methods Mol Biol 371:267-87. 2007
    ..This system is capable of detecting a broad range of mutational events, varying from small mutations in the lacZ reporter gene to large genome rearrangements with one breakpoint in lacZ and the other breakpoint elsewhere in the genome...
  76. ncbi Comparison between in vivo mutagenicity and carcinogenicity in multiple organs by benzo[a]pyrene in the lacZ transgenic mouse (Muta Mouse)
    A Hakura
    Drug Safety Research Laboratories, Ibaraki, Japan
    Mutat Res 398:123-30. 1998
    ..They also indicate, however, that the magnitude of the in vivo lacZ mutant frequencies induced by BP in different organs did not fully correlate with the target organs for carcinogenicity...
  77. pmc Influence of catabolite repression and inducer exclusion on the bistable behavior of the lac operon
    Moises Santillan
    Centre for Nonlinear Dynamics, and Departments of Physiology, Physics, and Mathematics, McGill University, H3G 1Y6 Montreal, Quebec, Canada
    Biophys J 86:1282-92. 2004
    A mathematical model of the lac operon which includes all of the known regulatory mechanisms, including external-glucose-dependent catabolite repression and inducer exclusion, as well as the time delays inherent to transcription and ..
  78. pmc Combinatorial transcriptional control of the lactose operon of Escherichia coli
    Thomas Kuhlman
    Center for Theoretical Biological Physics, University of California at San Diego, La Jolla, CA 92093 0374, USA
    Proc Natl Acad Sci U S A 104:6043-8. 2007
    ..Specifically, our analysis indicates that the sensitivity of the inducer response results from LacR-mediated DNA looping, which is significantly enhanced by CRP...
  79. ncbi lac operon induction in Escherichia coli: Systematic comparison of IPTG and TMG induction and influence of the transacetylase LacA
    Anja Marbach
    Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr 1, 39106 Magdeburg, Germany
    J Biotechnol 157:82-8. 2012
    Most commonly used expression systems in bacteria are based on the Escherichia coli lac promoter. Furthermore, lac operon elements are used today in systems and synthetic biology...
  80. ncbi Effects of different fixatives on beta-galactosidase activity
    Masafumi Takahashi
    J Histochem Cytochem 51:553-4. 2003
  81. ncbi Embedding of bone samples in methylmethacrylate: a suitable method for tracking LacZ mesenchymal stem cells in skeletal tissues
    D Hannouche
    Laboratoire de Recherches Orthopediques, CNRS, Faculte de Medecine Lariboisiere Saint Louis, Universite Paris 7, 75010 Paris, France
    J Histochem Cytochem 55:255-62. 2007
    ..LacZ is a suitable phenotypic marker to track MSCs in skeletal tissues embedded in MMA...
  82. ncbi Corneal development, limbal stem cell function, and corneal epithelial cell migration in the Pax6(+/-) mouse
    J Martin Collinson
    Department of Biomedical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, Scotland, United Kingdom
    Invest Ophthalmol Vis Sci 45:1101-8. 2004
    ..To investigate the etiology of corneal dysfunction in the Pax6(+/-) mouse model of aniridia-related keratopathy...
  83. ncbi Mechanism of CRP-cAMP activation of lac operon transcription initiation activation of the P1 promoter
    T P Malan
    J Mol Biol 180:881-909. 1984
    ..DNA supercoiling enhanced the promoter strength of the lac wild-type and UV5 promoters. The combination of supercoiling and CRP-cAMP was necessary for optimal promoter strength for the lac wild-type promoter...
  84. ncbi Syn, anti, and finally both conformations of cyclic AMP are involved in the CRP-dependent transcription initiation mechanism in E. coli lac operon
    Yusuf Tutar
    Department of Chemistry, Cumhuriyet University, Sivas, Turkey
    Cell Biochem Funct 26:399-405. 2008
    ..Therefore, the aim of this review is to summarize recent works and developments on the cAMP-dependent CRP activation mechanism in E. coli...
  85. ncbi Allostery and the lac Operon
    Mitchell Lewis
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, 37th and Hamilton Walk, Philadelphia, PA 19104, USA
    J Mol Biol 425:2309-16. 2013
    ..Over the past half-century, the details of the lac operon and the allosteric model have been tested using genetic, biochemical, and structural techniques...
  86. pmc Mechanism of transcriptional repression at a bacterial promoter by analysis of single molecules
    Alvaro Sanchez
    Graduate Program in Biophysics and Structural Biology, Brandeis University, Waltham, MA, USA
    EMBO J 30:3940-6. 2011
    ..The single-molecule statistical partitioning approach is broadly applicable to elucidating mechanisms of regulatory systems including those that are kinetically rather than thermodynamically controlled...
  87. ncbi Adaptive amplification: an inducible chromosomal instability mechanism
    P J Hastings
    Department of Molecular and Human Genetics Baylor College of Medicine One Baylor Plaza Houston, TX 77030, USA
    Cell 103:723-31. 2000
    ..We report the discovery of adaptive amplification at the lac operon in Escherichia coli...
  88. ncbi In vivo imaging of beta-galactosidase activity using far red fluorescent switch
    Ching Hsuan Tung
    Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    Cancer Res 64:1579-83. 2004
    ..The results indicated that in vivo real-time detection of beta-gal activity is possible by fluorescence imaging technology...
  89. ncbi Structure of the lac operon galactoside acetyltransferase
    Xing Guo Wang
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Structure 10:581-8. 2002
    ..3.1.18) is a gene product of the classical lac operon. GAT may assist cellular detoxification by acetylating nonmetabolizable pyranosides, thereby preventing their ..
  90. ncbi The tyrosinase related protein-1 (Tyrp1) promoter in transgenic experiments: targeted expression to the retinal pigment epithelium
    F Beermann
    ISREC Swiss Institute for Experimental Cancer Research, Epalinges
    Cell Mol Biol (Noisy-le-grand) 45:961-8. 1999
    ..Tyrp1-Tag transgenic mice developed malignant eye tumors of RPE origin, which invaded the optic nerve and led to metastasis into lymph nodes and spleen...
  91. pmc Quantitative approaches to the study of bistability in the lac operon of Escherichia coli
    Moises Santillan
    Centro de Investigacion y Estudios Avanzados del IPN, Unidad Monterrey, Parque de Investigación e Innovación Tecnológica, Apodaca, NL, Mexico
    J R Soc Interface 5:S29-39. 2008
    In this paper, the history and importance of the lac operon in the development of molecular and systems biology are briefly reviewed...
  92. ncbi Kinetics of induction of DNA damage and lacZ gene mutations in stomach mucosa of mice treated with beta-propiolactone and N-methyl-N'-nitro-N-nitrosoguanidine, using single-cell gel electrophoresis and MutaMouse models
    D Brault
    Rhone Poulenc Rorer, Nonclinical Safety Assessment, 13 quai Jules Guesde, BP 14, F 94403 Vitry sur Seine, France
    Environ Mol Mutagen 34:182-9. 1999
    ..These results illustrate the ability and complementarity of the SCGE and MutaMouse models to assess the genotoxicity of direct alkylating agents in the mouse gastric mucosa in vivo...
  93. ncbi Stochastic and deterministic simulations of heterogeneous cell population dynamics
    Nikos V Mantzaris
    Department of Chemical and Biomolecular Engineering and Bioengineering Department, Rice University, Houston, TX 77005, USA
    J Theor Biol 241:690-706. 2006
    ..kinetics and (b) the expression dynamics of a genetic network with positive feedback architecture, such as the lac operon. The effects of stochastic division as well as those of different division and partitioning mechanisms were ..
  94. ncbi Glucose lowers CRP* levels resulting in repression of the lac operon in cells lacking cAMP
    H Tagami
    Department of Molecular Biology, School of Science, Nagoya University, Japan
    Mol Microbiol 17:251-8. 1995
    ....
  95. ncbi Anionic liposomes increase the efficiency of adenovirus-mediated gene transfer to coxsackie-adenovirus receptor deficient cells
    Zhirong Zhong
    Key Laboratory of Drug Targeting and Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, P R China
    Mol Pharm 7:105-15. 2010
    ..In conclusion, anionic liposomes prepared by the calcium-induced phase change method could significantly enhance the transduction ability of Ad5 in CAR deficient cells...
  96. pmc Oxygen, nitrate, and molybdenum regulation of dmsABC gene expression in Escherichia coli
    P A Cotter
    Department of Microbiology, University of California, Los Angeles
    J Bacteriol 171:3817-23. 1989
    ..Additionally, expression of dmsABC varied depending on the carbon source. Expression was highest when cells were grown on sorbitol...
  97. ncbi Dynamics and bistability in a reduced model of the lac operon
    Necmettin Yildirim
    Centre for Nonlinear Dynamics, McGill University, Montreal, Quebec H3G 1Y6, Canada
    Chaos 14:279-92. 2004
    It is known that the lac operon regulatory pathway is capable of showing bistable behavior...
  98. pmc Lac repressor hinge flexibility and DNA looping: single molecule kinetics by tethered particle motion
    Francesco Vanzi
    LENS European Laboratory for Nonlinear Spectroscopy, University of Florence, Italy
    Nucleic Acids Res 34:3409-20. 2006
    ....
  99. ncbi Regulation of chondrocyte differentiation by Cbfa1
    I S Kim
    Universitäts Kinderklinik Mainz, Langenbeckstrasse 1, 55101, Mainz, Germany
    Mech Dev 80:159-70. 1999
    ..Our studies identify Cbfa1 as a major positive regulator of chondrocyte differentiation...
  100. ncbi Increased mutant frequency by carbon black, but not quartz, in the lacZ and cII transgenes of muta mouse lung epithelial cells
    Nicklas Raun Jacobsen
    National Research Centre for the Working Environment, Lersø Parkallé 105, DK 2100 Copenhagen Ø, Denmark
    Environ Mol Mutagen 48:451-61. 2007
    ..More studies are essential for understanding the biological significance of these findings, and clearly documenting DNA sequence changes. The results do not necessarily imply that other carbonaceous nano materials are genotoxic...
  101. pmc Surveying a supercoil domain by using the gamma delta resolution system in Salmonella typhimurium
    N P Higgins
    Department of Biochemistry, University of Alabama at Birmingham, 35294 2170, USA
    J Bacteriol 178:2825-35. 1996
    ..10(-0.02x). We found that res sites can be plectonemically interwound over long distances ( > 100 kb) and that barriers to supercoil diffusion are placed stochastically within the 43- to 45-min region of the chromosome...

Research Grants63

  1. A non-secreted form of IGF-1 is a protective factor for neural stem cells
    ANTHONY JOHN WINDEBANK; Fiscal Year: 2012
    ..We will take advantage of an inducible gene expression system that utilizes control elements from the lac operon of E. coli to induce or repress transgene expression in the mouse...
  2. Structures of DNA and RNA Polymerases and Functional Complexes
    THOMAS ARTHUR STEITZ; Fiscal Year: 2013
    ..coli will be examined from its structures complexed with the gal and lac operon promoter DNAs and the catabolite gene activator protein regulator transcription factor...
  3. Genetic circuits based on allosteric ribozymes
    Andrew D Ellington; Fiscal Year: 2010
    ..are instrumental in modulating the expression of operons involved in the utilization of sugars, such as the lac operon. Starting with three different ribozyme platforms (hammerhead, L1 ligase, and Group I self-splicing intron) we ..
  4. MUTAGENIC AND RECOMBINOGENIC EFFECTS OF GAMMA RAYS
    William Taylor; Fiscal Year: 1990
    ..Mutagenesis will be measured as forward mutations of the Lac gene in plasmid PBRPI which contains the E. coli Lac operon cloned into PBR322. The host cells in this case will be delta lac...
  5. ANTHRACYCLINE-INDUCED DNA SEQUENCE SPECIFIC MUTATION
    W Sedwick; Fiscal Year: 1993
    ..proposal is to investigate the basis of the mutagenic specificity of anthracyclines for the lacO region of the lac operon, in vitro and in procaryotic and eukaryotic systems...
  6. PRIMARY STRUCTURE OF THE CYCLIC AMP RECEPTOR PROTEIN
    David Schlesinger; Fiscal Year: 1980
    ..an allosteric protein which exerts a positive control on polycistronic mRNA protein synthesis of enzymes of the lac operon in response to intracellular levels of cAMP...
  7. Non-antiobiotic resistance markers for bacteria
    Herbert Schweizer; Fiscal Year: 2006
    ..cassettes will be constructed by placing the respective genes under the control of modified Escherichia coli lac operon promoters...
  8. CYANASE AND THE CYANASE OPERON
    Paul Anderson; Fiscal Year: 1993
    ..a permease for cyanate, cyanase, and a gene product of unknown function; the operon partially overlaps the lac operon, has been sequenced, and is controlled by a regulatory gene located only 108 base pairs upstream from the coding ..
  9. Transcription coupled DNA repair in E. Coli
    Philip Hanawalt; Fiscal Year: 2006
    ..III. We will measure DNA turnover in the undamaged lac operon when it is expressed, or repressed, comparing the frequency of "gratuitous" repair synthesis in each strand ..
  10. Mechanics of Transcriptional Control through DNA Looping
    Jens Christian Meiners; Fiscal Year: 2006
    ..For this aim, a simplified model system for DNA looping based on the lac repressor and the lac operon in E. coli will be studied in vitro in a series of single-molecule experiments...
  11. LAC REPRESSOR MOUSE
    Heidi Scrable; Fiscal Year: 2004
    ..the research proposed in this application is control of the endogenous mouse genome by elements derived from the lac operon of E. coli...
  12. SPECTROSCOPIC APPROACHES TO GENE REGULATION
    Ponzy Lu; Fiscal Year: 1990
    ..We will approach this using three systems: a) The operator of the lac operon; b) the two sets of lambda phage operators OR and OL...
  13. CONTROLLED REGULATION OF SV40 T AG SYNTHESIS
    JAMES DECAPRIO; Fiscal Year: 1992
    ..Coli lac operon function efficiently in animal cells...
  14. MECHANISM AND REGULATION OF E. COLI RNA POLYMERASE
    WILLIAM MCCLURE; Fiscal Year: 1980
    ..The mechanism of transcription activation will be investigated on the lac operon. We also want to know how ribosomal RNA and tRNA synthesis is controlled in E. coli...
  15. POSITIVE REGULATION OF METHIONINE BIOSYNTHESIS
    GEORGE STAUFFER; Fiscal Year: 1992
    ..The metE, metH and metR control regions have been fused to the lac operon. The metE-lac and metH-lac fusions will be used to isolate mutants with altered regulation by the positive ..
  16. BIOSYNTHESIS OF VITAMIN B12 AND ANAEROBIC METABOLISM
    John Roth; Fiscal Year: 2004
    ..coli. Understanding this metabolism may contribute to understanding the natural lifestyle of Salmonellae. ..
  17. Molecular mechanism of DNA-polymerase choice in vivo
    Susan Rosenberg; Fiscal Year: 2007
    ..coli DNA polymerases, and functions in DNA polymerase choice in vivo: a critical regulatory decision for cell survival of environmental damage, and genomic health. [unreadable] [unreadable] [unreadable]..
  18. DNA REPAIR IN DRUG RESISTANCE MUTATION
    Susan Rosenberg; Fiscal Year: 2003
    ....
  19. GENETIC CONTROL OF NITRATE RESPIRATION IN E. COLI
    Valley J Stewart; Fiscal Year: 2010
    ..Thus, results from the model species E. coli enhance understanding for a broad range of pathogens that significantly impact public health. ..
  20. Mutagenicity of tobacco smoke in human cell co-cultures
    Joseph Guttenplan; Fiscal Year: 2007
    ..Clearly, it is preferable to determine their potential risk prospectively, rather than retrospectively. [unreadable] [unreadable] [unreadable]..
  21. Origins of Spontaneous Mutagenesis
    Joseph Guttenplan; Fiscal Year: 2005
    ..abstract_text> ..
  22. Gene therapy for treatment of craniofacial dysplasia
    Stephanos Kyrkanides; Fiscal Year: 2004
    ..Completion of this study will aid in the development of novel therapies for the management of genetic disorders. ..
  23. CRANIOFACIAL DEVELOPMENT
    GURKIRPAL SOHAL; Fiscal Year: 2001
    ..Results of this study likely will alter our interpretations of mechanisms of normal development, and of the underlying causes of craniofacial anomalies, which make up about 25 percent of all malformations. ..
  24. Joint degeneration: Somatic mosaic analysis in a transgenic mouse
    Stephanos Kyrkanides; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  25. Large-scale chromatin structure and dynamics
    Andrew Belmont; Fiscal Year: 2007
    ..Knowledge gained from this research should provide valuable insight into regulation of basic molecular processes and may serve to guide design of future gene therapy vectors and stable transgene expression. ..
  26. Contributions of VENT cells to the GI tract
    GURKIRPAL SOHAL; Fiscal Year: 2007
    ..RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page .................................................................................................................................................. 1 Description, ..
  27. Does peripheral localized chronic inflammation predispose to neurodegeneration?
    Stephanos Kyrkanides; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  28. Recombinant FIV vectors for the delivery of siRNA therapy to joints
    Stephanos Kyrkanides; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  29. Mechanisms of Adaptive Amplification
    Philip J Hastings; Fiscal Year: 2010
    ....
  30. NEURONAL FUNCTION IN CRANIOFACIAL DEVELOPMENT
    Stephanos Kyrkanides; Fiscal Year: 2004
    ..abstract_text> ..
  31. Morphogenesis of the inner ear
    GURKIRPAL SOHAL; Fiscal Year: 2003
    ..abstract_text> ..
  32. REGULATION BY THE ESCHERICHIA COLI SMALL RNA, DSRA
    DARREN SLEDJESKI; Fiscal Year: 2002
    ..3) To compare the effect of DsrA and H-NS on DNA structure in vivo. In vivo footprinting of multicopy plasmid genes regulated by H-NS/DsrA will be used to compare the effects of hns-mutations and overexpression of DsrA on DNA structure. ..
  33. Hix in Enteric Mesenchymal and Neuronal Development
    Michael Bates; Fiscal Year: 2005
    ..abstract_text> ..
  34. Transcriptional Control of Early Coronary Vascular Development
    RAVINDRA P MISRA; Fiscal Year: 2010
    ....
  35. Functional Genetic Tests for Breast Cancer Diagnosis
    GRANT BITTER; Fiscal Year: 2003
    ..abstract_text> ..
  36. NEUTROPHIL RECRUITMENT IN LPS-INDUCED AIRWAY DISEASE
    Jessica Moreland; Fiscal Year: 2004
    ....
  37. Genome Evolution in Pathogens: The roles of predator-prey interactions
    Jeffrey G Lawrence; Fiscal Year: 2010
    ....
  38. Tandemly Repetitive Proteins in Mycoplasmas
    KEVIN F DYBVIG; Fiscal Year: 2010
    ..By understanding the role of repetitive proteins in the disease process, it is anticipated that improved measures to control the infections of these significant human pathogens will be developed. ..
  39. Transcriptional Integration of Metabolism
    ELTON YOUNG; Fiscal Year: 2007
    ..abstract_text> ..
  40. Pulsed magnetic fields, neurons, and blood vessels
    Diana Casper; Fiscal Year: 2006
    ..Results could have applications not only in therapeutic strategies for Parkinson's disease, but in the treatment of other chronic and acute vascular and neurodegenerative diseases. ..
  41. ROLES OF BMP4 AND ITS REGULATION IN TOOTH DEVELOPMENT
    Jian Feng; Fiscal Year: 2004
    ..Molecular techniques include RT-PCR, in situ hybridization, DNA sequence determination, SSCP (single strand conformational polymorphism), and heteroduplex analysis. ..
  42. SELENIUM-INDUCED GLAUCOMA
    W Stamer; Fiscal Year: 2006
    ..abstract_text> ..
  43. Antigen Identification in Occult Chorioretinopathies
    Jeffrey Bennett; Fiscal Year: 2006
    ....
  44. CONTROL OF CELL GROWTH
    Jonathan Gallant; Fiscal Year: 2005
    ..Because starvation conditions and the occurrence of a hungry codon may be frequent in nature, these phenomena are important to an understanding of translation and its control. ..
  45. Molecular Basis of Myocilin Function in the Human Eye
    W Daniel Stamer; Fiscal Year: 2010
    ..abstract_text> ..
  46. Gene Expression Patterns and Lifestyles in Legionella
    Howard Shuman; Fiscal Year: 2009
    ..abstract_text> ..
  47. Transplantation of Reduced-Size Fatty Livers
    Zhi Zhong; Fiscal Year: 2010
    ....
  48. CADHERIN DYNAMICS AND GLAUCOMA
    W Daniel Stamer; Fiscal Year: 2010
    ..Results obtained from these investigations will provide a basic understanding of the role of cadherin proteins in aqueous outflow resistance and uncover novel therapeutic targets for glaucoma therapy. ..
  49. ASM Conference on Candida and Candidiasis
    Aaron Mitchell; Fiscal Year: 2008
    ..The conference will help chart the course of future research and facilitate the urgent necessity to understand, treat, and prevent disease. [unreadable] [unreadable] [unreadable]..
  50. K12 Expression: Cornea-Type Epithelial Differentiation
    WINSTON KAO; Fiscal Year: 2008
    ..Aim 3 is to examine roles of Fos on corneal-type epithelium differentiation and corneal wound healing as described in Aim 2. ..
  51. Coordinated control of a regulatory network by gcvB RNA
    GEORGE STAUFFER; Fiscal Year: 2008
    ..Second, we will determine the scope of the regulatory network and the molecular mechanisms of control by the GcvB RNAs. [unreadable] [unreadable]..
  52. Endothelial Nitric Oxide Synthase & Portal Hypertension
    Vijay Shah; Fiscal Year: 2002
    ..abstract_text> ..
  53. RECOMBINOGENIC ENGINEERING OF PATHOGENIC BACTERIA
    KENAN MURPHY; Fiscal Year: 2003
    ....
  54. GENETIC RECOMBINATION IN MYCOPLASMAS
    Kevin Dybvig; Fiscal Year: 2004
    ..In aim 2, mutants with defects in putative DNA recombinases and V-I protein production will be examined to study the mechanisms of gene rearrangements and phenotypic switching. ..
  55. CADHERIN REGULATION OF AQUEOUS HUMOR OUTFLOW
    W Stamer; Fiscal Year: 2004
    ..abstract_text> ..
  56. Fucosylated cell surface molecules in tumor invasion
    STEVEN DOMINO; Fiscal Year: 2005
    ..and in 1,2-dimethylhydrazine-induced tumors, in the presence and absence of cell surface alpha(1,2)fucose by crossing mice into the Fut1 and Fut2 null background. ..
  57. Regulation and function of Pseudomonas drug efflux pumps
    Herbert Schweizer; Fiscal Year: 2006
    ..Aim 3: Probe other, perhaps global regulators of efflux operon expression, specifically MexS and PA4878. ..
  58. Imageable tumor-targeting bacteria
    Ming Zhao; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  59. Methionine Recycling Pathways in Klebsiella
    Jeffrey Lawrence; Fiscal Year: 2006
    ..In this way, characterization of these pathways sheds light on the evolution of complex metabolism, and on the selective forces that lead to gene acquisition, gene loss, and genome change. ..
  60. REGULATION OF E COLI CYTOCHROME OXIDASE AND TCA CYCLE
    Robert Gunsalus; Fiscal Year: 2001
    ..coli. These studies should provide a detailed understanding of how the cell coordinates pathways for electron and carbon flow within the cell during transition between oxygen rich and oxygen limiting conditions. ..