Genomes and Genes
sister chromatid exchange
Summary: An exchange of segments between the sister chromatids of a chromosome, either between the sister chromatids of a meiotic tetrad or between the sister chromatids of a duplicated somatic chromosome. Its frequency is increased by ultraviolet and ionizing radiation and other mutagenic agents and is particularly high in BLOOM SYNDROME.
Publications289 found, 100 shown here
- Induction of sister chromatid exchanges by the thymidine analog 5-hydroxymethyl-2'-deoxyuridineE R Kaufman
Department of Genetics, University of Illinois College of Medicine, Chicago 60612
Somat Cell Mol Genet 15:563-8. 1989..These findings support the hypothesis that the formation of hmUra residues in DNA may be an important factor in the genotoxicity of radiation and oxidative damage in mammalian cells...
- Molecular mechanisms of sister-chromatid exchangeDavid M Wilson
Laboratory of Molecular Gerontology, National Institute on Aging, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
Mutat Res 616:11-23. 2007..A more general overview of the cellular processes and key protein "effectors" that regulate the manifestation of SCE is also presented...
- Sister cohesion and structural axis components mediate homolog bias of meiotic recombinationKeun P Kim
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
Cell 143:924-37. 2010..These domains could comprise complementary environments that integrate inputs from DSB repair and mitotic chromosome morphogenesis into the complete meiotic program...
- Acetylation of Smc3 by Eco1 is required for S phase sister chromatid cohesion in both human and yeastJinglan Zhang
Center for Molecular Discovery, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
Mol Cell 31:143-51. 2008..Our study thus identified a molecular target for the acetyltransferase Eco1 and revealed that Smc3 acetylation is a conserved mechanism in regulating sister chromatid cohesion...
- Smc5-Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombinationGiacomo De Piccoli
Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK
Nat Cell Biol 8:1032-4. 2006..Our findings show that the Smc5-Smc6 complex is essential for genome stability as it promotes repair of DSBs by error-free sister-chromatid recombination (SCR), thereby suppressing inappropriate non-sister recombination events...
- Distinct roles of Mus81, Yen1, Slx1-Slx4, and Rad1 nucleases in the repair of replication-born double-strand breaks by sister chromatid exchangeSandra Muñoz-Galván
Centro Andaluz de Biologia Molecular y Medicina Regenerativa, Universidad de Sevilla CSIC, Seville, Spain
Mol Cell Biol 32:1592-603. 2012..These unique effects, which have not been detected in other studies unless double mutant combinations were used, indicate the formation of distinct alternatives for the repair of replication-born DSBs that require specific SSEs...
- Loss of Rap1 induces telomere recombination in the absence of NHEJ or a DNA damage signalAgnel Sfeir
The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
Science 327:1657-61. 2010..The data reveal that HDR at telomeres can take place in the absence of DNA damage foci and underscore the functional compartmentalization within shelterin...
- Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosisZhanyun Tang
Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9041, USA
Proc Natl Acad Sci U S A 101:18012-7. 2004..Bub1 maintains the steady-state levels and centromeric localization of Sgo1. Therefore, Bub1 protects centromeric cohesion through Shugoshin in mitosis...
- Absence of genotoxicity in human blood cells exposed to 50 Hz magnetic fields as assessed by comet assay, chromosome aberration, micronucleus, and sister chromatid exchange analysesL Stronati
Section of Toxicology and Biomedical Sciences, ENEA CR, Casaccia, Rome, Italy
Bioelectromagnetics 25:41-8. 2004..Moreover, no synergistic effect with ionizing radiation has been observed. A slight but significant decrease of cell proliferation was evident in ELFMFs treated samples and samples subjected to the combined exposure...
- DNA-PK-dependent RPA2 hyperphosphorylation facilitates DNA repair and suppresses sister chromatid exchangeHungjiun Liaw
Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America
PLoS ONE 6:e21424. 2011..This pathway thus permits cells to repair DNA damage properly and increase cell viability...
- Hyper telomere recombination accelerates replicative senescence and may promote premature agingR Tanner Hagelstrom
Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, 80523 1618, USA
Proc Natl Acad Sci U S A 107:15768-73. 2010..To gain insight into the origin of cellular senescence and human aging, we analyzed the dependence of sister chromatid exchange (SCE) frequencies on location [i.e., genomic (G-SCE) vs...
- RFCCtf18 and the Swi1-Swi3 complex function in separate and redundant pathways required for the stabilization of replication forks to facilitate sister chromatid cohesion in Schizosaccharomyces pombeAlison B Ansbach
Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, USA
Mol Biol Cell 19:595-607. 2008..We propose that RFC(Ctf18) and the Swi1-Swi3 complex function in separate and redundant pathways essential for replication fork stabilization to facilitate sister chromatid cohesion in fission yeast...
- Chiasmata promote monopolar attachment of sister chromatids and their co-segregation toward the proper pole during meiosis IYukinobu Hirose
The Department of Chemistry, Shizuoka University, Shizuoka, Japan
PLoS Genet 7:e1001329. 2011..Based on these results, we propose that chiasmata play a pivotal role in the selection of proper attachments and provide a backup mechanism that promotes correct chromosome segregation when improper attachments remain during anaphase I...
- DNA methyltransferases control telomere length and telomere recombination in mammalian cellsSusana Gonzalo
Telomeres and Telomerase Group, Molecular Oncology Program, Spanish National Cancer Centre CNIO, Madrid E 28029, Spain
Nat Cell Biol 8:416-24. 2006..Together, these results demonstrate a previously unappreciated role for DNA methylation in maintaining telomere integrity...
- Eme1 is involved in DNA damage processing and maintenance of genomic stability in mammalian cellsJacinth Abraham
Advanced Medical Discovery Institute, Ontario Cancer Institute, 620 University Avenue, Suite 706, Toronto, Ontario M5G 2C1, Canada
EMBO J 22:6137-47. 2003..that arise during homologous recombination processes such as gene targeting, gene conversion and sister chromatid exchange (SCE)...
- Modulation of Escherichia coli sister chromosome cohesion by topoisomerase IVXindan Wang
Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
Genes Dev 22:2426-33. 2008..Therefore, we propose that precatenanes, which form as replication progresses by interwinding of newly replicated sister chromosomes, are responsible for E. coli sister chromosome cohesion...
- Role of the Saccharomyces cerevisiae Rad51 paralogs in sister chromatid recombinationAmy M Mozlin
Department of Microbiology, Columbia University Medical Center, New York, New York 10032, USA
Genetics 178:113-26. 2008..Furthermore, these data suggest that most spontaneous SCR initiates from single-stranded gaps formed at stalled replication forks rather than DSBs...
- Assaying and investigating Alternative Lengthening of Telomeres activity in human cells and cancersJeremy D Henson
Children s Medical Research Institute, Sydney, NSW, Australia
FEBS Lett 584:3800-11. 2010..The prevalence of ALT ranges from 25% to 60% in sarcomas and 5% to 15% in carcinomas. Patients with many of these types of ALT[+] tumors have a poor prognosis...
- Unified mode of centromeric protection by shugoshin in mammalian oocytes and somatic cellsJibak Lee
Laboratory of Reproductive Biology and Biotechnology, Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
Nat Cell Biol 10:42-52. 2008..These results argue for a unified view of centromeric protection by shugoshin in mitosis and meiosis...
- RECQL, a member of the RecQ family of DNA helicases, suppresses chromosomal instabilitySudha Sharma
NIEHS MD A2 05, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
Mol Cell Biol 27:1784-94. 2007..Genetic background, functional redundancy, and perhaps other factors may protect the unstressed mouse from the types of abnormalities that might be expected from the severe chromosomal aberrations detected at the cellular level...
- Different genetic requirements for repair of replication-born double-strand breaks by sister-chromatid recombination and break-induced replicationFelipe Cortes-Ledesma
Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, and Departamento de Biología Molecular, CABIMER, CSIC Universidad de Sevilla, Av Americo Vespucio s n, 41092 Sevilla, Spain
Nucleic Acids Res 35:6560-70. 2007..Our study provides molecular evidence for inversions between repeats occurring by BIR followed by single-strand annealing (SSA) in the absence of strand exchange...
- The absence of the yeast chromatin assembly factor Asf1 increases genomic instability and sister chromatid exchangeFelix Prado
Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avda Reina Mercedes 6, 41012 Sevilla, Spain
EMBO Rep 5:497-502. 2004..In addition, asf1Delta shows high levels of sister chromatid exchange (SCE) and is proficient in DSB-induced SCE as determined by physical analysis...
- The Dot1 histone methyltransferase and the Rad9 checkpoint adaptor contribute to cohesin-dependent double-strand break repair by sister chromatid recombination in Saccharomyces cerevisiaeFrancisco Conde
Universidad de Salamanca, Salamanca, Spain
Genetics 182:437-46. 2009..Therefore, our results contribute to an understanding of how different chromatin modifications impinge on DNA repair mechanisms, which are fundamental for maintaining genomic stability...
- The ELG1 clamp loader plays a role in sister chromatid cohesionOren Parnas
Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel
PLoS ONE 4:e5497. 2009..Our results suggest that Elg1, Ctf4, and Ctf18 may coordinate the relative movement of the replication fork with respect to the cohesin ring...
- BRCA1 regulates RAD51 function in response to DNA damage and suppresses spontaneous sister chromatid replication slippage: implications for sister chromatid cohesion, genome stability, and carcinogenesisIsabelle Cousineau
Molecular Biology Program and Department of Biochemistry, Universite de Montreal, Quebec, Canada
Cancer Res 65:11384-91. 2005..Loss of such control in response to estrogen-induced DNA damage after BRCA1 inactivation may be a key initial event that triggers genome instability and carcinogenesis...
- Mouse centric and pericentric satellite repeats form distinct functional heterochromatinMounia Guenatri
Institut Curie Research section, UMR218 du Centre National pour la Recherche Scientifique, 26 Rue d Ulm, 75248 Paris Cedex 05, France
J Cell Biol 166:493-505. 2004..Thus, we define functionally independent centromeric subdomains, which spatio-temporal isolation is proposed to be important for centromeric cohesion and dissociation during chromosome segregation...
- Role of ATM in the telomere response to the G-quadruplex ligand 360AGaëlle Pennarun
CEA, DSV, IRCM, Laboratoire de Radiopathologie IPSC, 92265 Fontenay aux Roses, France
Nucleic Acids Res 36:1741-54. 2008..Our results suggest thus that ATM has a direct role in preventing inappropriate DNA repair at telomeres, which could be related to its possible participation to the formation of protected structures at telomeres...
- Early embryonic lethality due to targeted inactivation of DNA ligase IIINahum Puebla-Osorio
Department of Immunology, Unit 902, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Blvd, Houston, Texas 77030, USA
Mol Cell Biol 26:3935-41. 2006..5 dpc. LIG3 mutant cells have relatively normal XRCC1 levels but elevated sister chromatid exchange. These findings indicate that DNA ligase III is involved in essential DNA repair activities required for ..
- Effects on sister chromatid exchange frequency of polymorphisms in DNA repair gene XRCC1 in smokersYu Chen Lei
Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, No 1 Ren Ai Road, Sec 1, 10018 Taipei, ROC
Mutat Res 519:93-101. 2002..if the polymorphisms of metabolic traits and DNA repair modulate smoking-related DNA damage, we used sister chromatid exchange (SCE) as a marker of genetic damage to explore the relationship of microsomal epoxide hydrolase (mEH), ..
- Relationship between DNA lesions, DNA repair and chromosomal damage induced by acetaldehydeClaudia Lorenti Garcia
Department of Agrobiology and Agrochemistry, University of Tuscia, Via S C De Lellis snc, I 01100 Viterbo, Italy
Mutat Res 662:3-9. 2009..TCR/NER pathways are involved in repairing FA induced DPCs, but less in AA-induced DPCs. Our preliminary data suggest that DPCs are weaker inducers of SCEs in comparison with ICLs...
- Rqh1 blocks recombination between sister chromatids during double strand break repair, independent of its helicase activityJustin C Hope
Graduate Program in Anatomy and Cell Biology, Columbia University, Kolb Building Room 140, 722 West 168th Street, New York, NY 10032, USA
Proc Natl Acad Sci U S A 103:5875-80. 2006..In the course of these studies, we also found nonhomologous end joining to be largely faithful in S. pombe, contrary to current belief. These findings provide insight into the regulation of DSB repair by RecQ helicases...
- S-phase and DNA damage activated establishment of sister chromatid cohesion--importance for DNA repairCamilla Sjögren
Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden
Exp Cell Res 316:1445-53. 2010..Here, we present and discuss the current models for establishment of S-phase and DI-cohesion in the context of their involvement in DSB repair...
- Repositioning of aurora B promoted by chiasmata ensures sister chromatid mono-orientation in meiosis ITakeshi Sakuno
Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences Promotion of Independence for Young Investigators, University of Tokyo, Yayoi, Tokyo 113 0032, Japan
Dev Cell 21:534-45. 2011..Our results elucidate how chiasmata favor biorientation of bivalents over that of univalents at meiosis I...
- Cellular and molecular consequences of defective Fanconi anemia proteins in replication-coupled DNA repair: mechanistic insightsLarry H Thompson
Biology and Biotechnology Division, L452, Lawrence Livermore National Laboratory, P O Box 808, Livermore, CA 94551 0808, United States
Mutat Res 668:54-72. 2009..The FANCJ/BRIP1/BACH1 helicase functions in association with BRCA1 and may remove structural barriers to replication, such as guanine quadruplex structures, and/or assist in crosslink unhooking...
- Investigation of Arg399Gln and Arg194Trp polymorphisms of the XRCC1 (x-ray cross-complementing group 1) gene and its correlation to sister chromatid exchange frequency in patients with chronic lymphocytic leukemiaNilgun Duman
Department of Internal Medicine, Division of Medical Genetics, Istanbul University, Istanbul Medical Faculty, Istanbul, Turkey
Genet Test Mol Biomarkers 16:287-91. 2012..We also analyzed their relation to frequency of sister chromatid exchange (SCE)...
- Sister chromatid exchange and proliferative rate index in the longitudinal risk assessment of occupational exposure to pesticidesDavor Zeljezic
Laboratory for Mutagenesis, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Chemosphere 46:295-303. 2002..genetic damage on a population of workers occupationally exposed to a mixture of pesticides by using sister chromatid exchange (SCE) analysis has been evaluated...
- Shugoshin prevents dissociation of cohesin from centromeres during mitosis in vertebrate cellsBarry E McGuinness
Research Institute of Molecular Pathology, Vienna, Austria
PLoS Biol 3:e86. 2005..Centromeric cohesion is one of the hallmarks of mitotic chromosomes. Our results imply that it is not an intrinsically stable property, because it can easily be destroyed by mitotic kinases, which are kept in check by shugoshin...
- Mutational analysis of the Drosophila sister-chromatid cohesion protein ORD and its role in the maintenance of centromeric cohesionS E Bickel
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
Genetics 146:1319-31. 1997..Therefore, ORD activity appears to promote centromeric cohesion during meiosis II but is not essential for kinetochore function during anaphase...
- XRCC1 down-regulation in human cells leads to DNA-damaging agent hypersensitivity, elevated sister chromatid exchange, and reduced survival of BRCA2 mutant cellsJinshui Fan
Laboratory of Molecular Gerontology, National Institute on Aging, Baltimore, Maryland, USA
Environ Mol Mutagen 48:491-500. 2007..reduced single-strand break repair, increased sensitivity to DNA-damaging agents, and elevated levels of sister chromatid exchange (SCE)...
- Fanconi anemia protein FANCD2 promotes immunoglobulin gene conversion and DNA repair through a mechanism related to homologous recombinationKazuhiko Yamamoto
Department of Immunology and Molecular Genetics, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama 701 0192, Japan
Mol Cell Biol 25:34-43. 2005..In contrast to these defects, we also found increased spontaneous sister chromatid exchange (SCE) and intact Rad51 foci formation after DNA damage...
- Cell-cycle regulation of cohesin stability along fission yeast chromosomesPascal Bernard
Universite Bordeaux 2, CNRS Institut de Biochimie et Génétique Cellulaires, Bordeaux, France
EMBO J 27:111-21. 2008..Hence, cohesin stabilization might be a pre-requisite for cohesion establishment rather than its consequence...
- Securin is required for chromosomal stability in human cellsP V Jallepalli
The Johns Hopkins Oncology Center, 1650 Orleans Street, Baltimore, MD 21231, USA
Cell 105:445-57. 2001..These results illuminate the function of mammalian securin and show that it is essential for the maintenance of euploidy...
- Sister chromatid separation at human telomeric regionsMichal Yalon
Bruce Rappaport Faculty of Medicine and Research Institute Technion and Rambam Medical Center, Haifa, Israel 31096
J Cell Sci 117:1961-70. 2004..Persistent cohesion at telomeres may be associated with attempted DNA repair or chromosomal abnormalities, which have been described in pre-senescent cells...
- Transient ATM kinase inhibition disrupts DNA damage-induced sister chromatid exchangeJason S White
Department of Radiation Oncology, University of Pittsburgh Medical School, Hillman Cancer Center, Research Pavilion, Suite 2 6, Pittsburgh, PA 15213 1863, USA
Sci Signal 3:ra44. 2010..acted through distinct DNA repair mechanisms: ATM resolved DNA damage through a mechanism involving sister chromatid exchange (SCE), whereas DNA-PK acted through nonhomologous end joining...
- Cellular characterization of cells from the Fanconi anemia complementation group, FA-D1/BRCA2Barbara C Godthelp
Department of Toxicogenetics, Leiden University Medical Center, Building 2, Postzone S 6 P, 2300 RC, Leiden, The Netherlands
Mutat Res 601:191-201. 2006..In contrast to Brca2-deficient rodent cells, FA-D1/BRCA2 cells showed normal sister chromatid exchange (SCE) levels, both spontaneous as well as after MMC treatment...
- Ku70 stimulates fusion of dysfunctional telomeres yet protects chromosome ends from homologous recombinationGiulia B Celli
Laboratory for Cell Biology and Genetics, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Nat Cell Biol 8:885-90. 2006..Therefore, Ku- and TRF2-mediated repression of homologous recombination is an important aspect of telomere protection...
- Sister chromatids are often incompletely aligned in meristematic and endopolyploid interphase nuclei of Arabidopsis thalianaVeit Schubert
Institute of Plant Genetics and Crop Plant Research IPK, D 06466 Gatersleben, Germany
Genetics 172:467-75. 2006....
- Atypically low spontaneous sister chromatid exchange formation in uveal melanomaLeslie Hoh
Academic Unit of Ophthalmology and Orthoptics, University of Sheffield, Sheffield S10 2RX, UK
Genes Chromosomes Cancer 50:34-42. 2011..cell lines and blood from cancer patients has universally been associated with an increased level of sister chromatid exchange (SCE)...
- The Chinese hamster FANCG/XRCC9 mutant NM3 fails to express the monoubiquitinated form of the FANCD2 protein, is hypersensitive to a range of DNA damaging agents and exhibits a normal level of spontaneous sister chromatid exchangeJ B Wilson
Mammalian DNA Repair Laboratory, School of Biological Sciences, Donnan Laboratories, University of Liverpool, Liverpool, L69 7ZD, UK
Carcinogenesis 22:1939-46. 2001..NM3, like human FA cell lines (but unlike UV40) exhibits a normal spontaneous level of sister chromatid exchange. We show that both NM3 and UV40 are also hypersensitive to other DNA crosslinking agents (including ..
- Insights into the mechanisms of sister chromatid exchange formationA Wojcik
Institute of Nuclear Chemistry and Technology, Warszawa, Poland
Cytogenet Genome Res 104:304-9. 2004..This technique was applied to study the SCEs induced by ionizing and UV radiation as well as by mitomycin C, DNaseI and AluI. This article is a review of the results and conclusions of our previous studies...
- Single Holliday junctions are intermediates of meiotic recombinationGareth A Cromie
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, P O Box 19024, Seattle, WA 98109, USA
Cell 127:1167-78. 2006....
- Homologous recombination is responsible for cell death in the absence of the Sgs1 and Srs2 helicasesS Gangloff
CEA de Fontenay aux Roses, UMR 217 CNRS CEA DSV DRR LERA, 92265 Fontenay aux Roses, France
Nat Genet 25:192-4. 2000..Yeast SRS2 encodes another DNA helicase involved in the maintenance of genome integrity. Our data suggest that some defects observed in BS, WS or RTS are the consequence of unrestrained recombination...
- [Effects of sevofluran on cell division and levels of sister chromatid exchange]N Luleci
Celal Bayar Universität, Medizinische Fakultat, Anästhesiologie Abteilung
Anasthesiol Intensivmed Notfallmed Schmerzther 40:213-6. 2005Purpose of the study was to investigate the mitotic index (MI) and sister chromatid exchange (SCE) levels to identify the mutagenic and carcinogenic effects of sevoflurane (sevoflurane).
- Increased rates of spontaneous sister chromatid exchange in lymphocytes of BRCA2+/- carriers of familial breast cancer clustersMin Kyoung Kim
Deutsches Krebsforschungszentrum, Abteilung Tumorgenetik B030, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Cancer Lett 210:85-94. 2004....
- Werner and Bloom helicases are involved in DNA repair in a complementary fashionOsamu Imamura
AGENE Research Institute, 200 Kajiwara, Kamakura, Kanagawa 247 0063, Japan
Oncogene 21:954-63. 2002..They also showed a threefold increase in targeted integration rate of exogenous DNAs, but not in sister chromatid exchange (SCE) frequency...
- Heterochromatin-mediated association of achiasmate homologs declines with age when cohesion is compromisedVijayalakshmi V Subramanian
Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
Genetics 181:1207-18. 2009..In addition, our data support the model that deterioration of meiotic cohesion during the aging process compromises the segregation of achiasmate as well as chiasmate bivalents...
- Genotoxic effects of a particular mixture of acetamiprid and alpha-cypermethrin on chromosome aberration, sister chromatid exchange, and micronucleus formation in human peripheral blood lymphocytesAyşe Yavuz Kocaman
Department of Biology, Faculty of Science and Letters, Mustafa Kemal University, Hatay, Turkey
Environ Toxicol 25:157-68. 2010..insecticide) on human peripheral lymphocytes were examined in vitro by chromosomal aberrations (CAs), sister chromatid exchange (SCE), and micronucleus (MN) tests. The human peripheral lymphocytes were treated with 12.5 + 2...
- Correlation of sister chromatid exchange formation through homologous recombination with ribonucleotide reductase inhibitionAtsuko Matsuoka
Division of Medical Devices, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya Ku, Tokyo 158 8501, Japan
Mutat Res 547:101-7. 2004We conducted the recombination and sister chromatid exchange (SCE) assays with five chemicals (hydroxyurea (HU), resveratrol, 4-hydroxy-trans-stilbene, 3-hydroxy-trans-stilbene, and mitomycin C) in Chinese hamster cell line SPD8/V79 to ..
- BLM ortholog, Sgs1, prevents aberrant crossing-over by suppressing formation of multichromatid joint moleculesSteve D Oh
Section of Microbiology, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA
Cell 130:259-72. 2007..Thus, by disrupting aberrant JMs, BLM-related helicases maximize repair efficiency while minimizing the risk of deleterious crossing-over...
- Elevated sister chromatid exchange frequencies in New Zealand Vietnam War veteransR E Rowland
Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand
Cytogenet Genome Res 116:248-51. 2007..group of 24 New Zealand Vietnam War veterans and 23 control volunteers were compared using an SCE (sister chromatid exchange) analysis. The results from the SCE study show a highly significant difference (P < 0...
- The effects of etodolac, nimesulid and naproxen sodium on the frequency of sister chromatid exchange after enclused third molars surgeryBanu Gurkan Koseoglu
Department of Oral Surgery, Istanbul University, Faculty of Dentistry, Istanbul, Turkey
Yonsei Med J 49:742-7. 2008..The evaluation of the frequency of sister chromatid exchange (SCE) is accepted as a reliable cytogenetic method to assess the genotoxic effects of environmental ..
- XRCC1, CYP2E1 and ALDH2 genetic polymorphisms and sister chromatid exchange frequency alterations amongst vinyl chloride monomer-exposed polyvinyl chloride workersRuey Hong Wong
Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, No 1 Ren Ai Rd, Sec 1, 10018 Taipei, Taiwan
Arch Toxicol 77:433-40. 2003..This study was designed to investigate whether metabolic and DNA-repair genotypes affected sister chromatid exchange (SCE) frequency in occupationally VCM-exposed workers from polyvinyl chloride (PVC) manufacturing plants...
- The association between frequencies of mitomycin C-induced sister chromatid exchange and cancer risk in arseniasisSaou Hsing Liou
Department of Public Health, National Defense Medical Center, National Defense University, Nei Hu, Taipei, Taiwan, ROC
Toxicol Lett 129:237-43. 2002..whether biomarkers of cytogenetic damage and susceptibility, such as spontaneous and mitomycin C-induced sister chromatid exchange (SCE) can predict cancer development, a nested case-control study was performed in a blackfoot endemic ..
- Sister chromatid exchange induction by the herbicide 2,4-dichlorophenoxyacetic acid in chick embryosElio Arias
Dipartimento di Biologia, Universita degli Studi di Milano, via G Celoria 26, I 20133 Milan, Italy
Ecotoxicol Environ Saf 55:338-43. 2003..In the present study, sister chromatid exchange (SCE) induction and cell cycle kinetics alterations by 2,4-D in 4-day old chick embryos were evaluated...
- The genotoxic effect of potassium metabisulfite using chromosome aberration, sister chromatid exchange, micronucleus tests in human lymphocytes and chromosome aberration test in bone marrow cells of ratsAyse Yavuz-Kocaman
Department of Biology, Natural and Applied Science Institute, Cukurova University, Adana, Turkey
Environ Mol Mutagen 49:276-82. 2008..The compound induced CA as well and decreased the MI in bone marrow cells of rats. It might be concluded that PMB had a high genotoxic and cytotoxic risk...
- Termini of human chromosomes display elevated rates of mitotic recombinationM N Cornforth
Department of Radiation Oncology, 344 Gail Borden Building, University of Texas Medical Branch, Galveston, TX 77555 0656, USA
Mutagenesis 16:85-9. 2001..Although the process of sister chromatid exchange (SCE) is the most prominent type of recombination in somatic mammalian cells, our results show that SCEs ..
- Elevated sister chromatid exchange frequencies in dividing human peripheral blood lymphocytes exposed to 50 Hz magnetic fieldsM A Wahab
Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand
Bioelectromagnetics 28:281-8. 2007The in vitro cytomolecular technique, sister chromatid exchange (SCE), was applied to test the clastogenic potentiality of extremely low frequency (ELF) electromagnetic fields (EMFs) on human peripheral blood lymphocytes (HPBLs)...
- The genotoxic and oxidative damage potential of olanzapine in vitroHasan Turkez
Biology Department, Science Faculty, Ataturk University, Erzurum, Turkey
Toxicol Ind Health 26:583-8. 2010..It is concluded that the OLZ can be used safely, but it is necessary to consider the tissue damages that are likely to appear depending on the oxidative stress...
- The effects of thymol on sister chromatid exchange, chromosome aberration and micronucleus in human lymphocytesMehmet Buyukleyla
Department of Biology, Natural and Applied Science Institute, Cukurova University, 01330 Adana, Turkey
Ecotoxicol Environ Saf 72:943-7. 2009..with 25, 50, 75, and 100 microg/ml concentrations of thymol for 24 and 48h treatment periods by using sister chromatid exchange (SCE), chromosome aberration (CA), and micronucleus (MN) tests...
- The PP2A inhibitor I2PP2A is essential for sister chromatid segregation in oocyte meiosis IIJean Philippe Chambon
UPMC University of Paris 6, 75005 Paris, France
Curr Biol 23:485-90. 2013..Our findings demonstrate that in oocytes I2PP2A is essential for faithful sister chromatid segregation by mediating deprotection of centromeric cohesin in meiosis II...
- Elevated sister chromatid exchange phenotype of Bloom syndrome cells is complemented by human chromosome 15L D McDaniel
Division of Human Genetics and Medical Biotechnology Center, University of Maryland, Baltimore 21201
Proc Natl Acad Sci U S A 89:7968-72. 1992..Detailed characterization revealed this complementing element to be human chromosome 15...
- Tipin/Tim1/And1 protein complex promotes Pol alpha chromatin binding and sister chromatid cohesionAlessia Errico
Genome Stability and Cell Cycle Control Groups, Cancer Research UK, Clare Hall Laboratories, South Mimms, Herts, UK
EMBO J 28:3681-92. 2009..These data indicate that Tipin/Tim1/And1 form a complex that links stabilization of replication fork and establishment of sister chromatid cohesion...
- SIR2 regulates recombination between different rDNA repeats, but not recombination within individual rRNA genes in yeastTakehiko Kobayashi
National Institute for Basic Biology, Okazaki, Japan
Cell 117:441-53. 2004..From these and other experiments, we conclude that SIR2 prevents unequal sister-chromatid recombination, probably by forming special cohesin structures, without significant effects on recombinational events within individual rRNA genes...
- Sex chromosome loss, micronuclei, sister chromatid exchange and aging: a study including 16 centenariansN Bukvic
DIMIMP Sezione di Genetica Medica, Policlinico, P.zza G. Cesare, 70124 Bari, Italy
Mutat Res 498:159-67. 2001..we analysed the possible effect of age, sex and smoking on the mean values of micronucleus (MN) and sister chromatid exchange (SCE) frequencies on peripheral blood obtained from 38 subjects ranging in age from 16 to 63 years and ..
- Functional relationships of FANCC to homologous recombination, translesion synthesis, and BLMSeiki Hirano
Department of Immunology and Molecular Genetics, Kawasaki Medical School, Kurashiki, Okayama, Japan
EMBO J 24:418-27. 2005Some of the restarting events of stalled replication forks lead to sister chromatid exchange (SCE) as a result of homologous recombination (HR) repair with crossing over...
- Multiple roles of Rev3, the catalytic subunit of polzeta in maintaining genome stability in vertebratesEiichiro Sonoda
CREST Research Project, Japan Science and Technology, Radiation Genetics, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
EMBO J 22:3188-97. 2003..REV3(-/-) cells showed significant increase in sister chromatid exchange events and chromosomal breaks even in the absence of exogenous genotoxic stress...
- Human RECQ1 is a DNA damage responsive protein required for genotoxic stress resistance and suppression of sister chromatid exchangesSudha Sharma
Laboratory of Molecular Gerontology, Department of Health and Human Services, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, United States of America
PLoS ONE 2:e1297. 2007....
- Double-strand-break-induced homologous recombination in mammalian cellsR D Johnson
Cell Biology Program, Memorial Sloan-Kettering Cancer Center and Cornell University Graduate School of Medical Sciences, 1275 York Avenue, New York, NY 10021
Biochem Soc Trans 29:196-201. 2001..To date, four mammalian proteins have been demonstrated conclusively to be involved in DSB repair by homologous recombination: Rad54, XRCC2, XRCC3 and BRCA1. This paper summarizes results from a number of recent studies...
- Telomeric recombination in mismatch repair deficient human colon cancer cells after telomerase inhibitionOliver E Bechter
University of Innsbruck, Department of Internal Medicine, Innsbruck, Austria
Cancer Res 64:3444-51. 2004..This is the first report of inducing a telomerase-independent telomere elongation in human cancer cells when telomerase is inhibited, thus describing a novel mechanism of resistance to antitelomerase therapy...
- Involvement of mammalian Mus81 in genome integrity and tumor suppressionJohn Peter McPherson
Ontario Cancer Institute, 620 University Avenue, Suite 706, Toronto, Ontario, Canada M5G 2C1
Science 304:1822-6. 2004..These studies demonstrate a critical role for the proper biallelic expression of the mammalian Mus81 in the maintenance of genomic integrity and tumor suppression...
- Zscan4 regulates telomere elongation and genomic stability in ES cellsMichal Zalzman
Developmental Genomics and Aging Section, Laboratory of Genetics, NIH, Baltimore, Maryland 21224, USA
Nature 464:858-63. 2010..Furthermore, Zscan4 knockdown shortens telomeres, increases karyotype abnormalities and spontaneous sister chromatid exchange, and slows down cell proliferation until reaching crisis by passage eight...
- Multiple organ system defects and transcriptional dysregulation in the Nipbl(+/-) mouse, a model of Cornelia de Lange SyndromeShimako Kawauchi
Department of Anatomy and Neurobiology, University of California Irvine, Irvine, California, United States of America
PLoS Genet 5:e1000650. 2009..In addition, evidence is presented that reduced expression of genes involved in adipogenic differentiation may underlie the low amounts of body fat observed both in Nipbl+/- mice and in individuals with CdLS...
- Double-strand breaks arising by replication through a nick are repaired by cohesin-dependent sister-chromatid exchangeFelipe Cortes-Ledesma
Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avd Reina Mercedes 6, 41012 Sevilla, Spain
EMBO Rep 7:919-26. 2006..This work opens new perspectives to understand the importance of single-strand breaks as a source of recombination and the relevance of cohesion in the repair of replication-born DSBs...
- Evaluation of chromosome aberrations, sister chromatid exchange and micronuclei in patients with type-1 diabetes mellitusNilüfer Cinkilic
Uludag University Science and Arts Faculty, Biology Department, Gorukle, Bursa, Turkey
Mutat Res 676:1-4. 2009..In the present study, we aimed to investigate the frequency of sister chromatid exchange (SCE), chromosomal aberrations (CA) and micronuclei (MN) in type-1 diabetes mellitus patients compared ..
- Requirement for the Xrcc1 DNA base excision repair gene during early mouse developmentR S Tebbs
Department of Dermatology, University of California at San Francisco, San Francisco, California, 94143, USA
Dev Biol 208:513-29. 1999....
- The Bloom's syndrome helicase can promote the regression of a model replication forkChristine Ralf
Cancer Research UK Laboratories, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DS, United Kingdom
J Biol Chem 281:22839-46. 2006..These data establish the existence of a eukaryotic protein that could promote replication fork regression in vivo and suggest a novel pathway through which BLM might suppress genetic exchanges...
- Roles of the sister chromatid cohesion apparatus in gene expression, development, and human syndromesDale Dorsett
Edward A Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, Saint Louis, MO 63104, USA
Chromosoma 116:1-13. 2007..Effects on chromatid cohesion are apparent in RBS/SC syndrome, but data from yeast and Drosophila point to the likelihood that changes in expression of genes located in heterochromatin could contribute to the developmental deficits...
- Recql5 and Blm RecQ DNA helicases have nonredundant roles in suppressing crossoversYiduo Hu
Department of Genetics, Case Western Reserve University, BRB, 7th Floor, 10900 Euclid Ave, Cleveland, OH 44106, USA
Mol Cell Biol 25:3431-42. 2005..Bloom syndrome homologue (Blm) or the Recql5 genes result in a significant increase in the frequency of sister chromatid exchange (SCE), whereas deleting both Blm and Recql5 lead to an even higher frequency of SCE...
- Bloom helicase is involved in DNA surveillance in early S phase in vertebrate cellsO Imamura
AGENE Research Institute, 200 Kajiwara, Kamakura, Kanagawa 247-0063, Japan
Oncogene 20:1143-51. 2001..mutator phenotypes such as hyper-recombination, chromosome instability and an increased frequency of sister chromatid exchange (SCE). To define the primary role of BLM, we generated BLM(-/-) mutants of the chicken B-cell line DT40...
- XRCC1 keeps DNA from getting strandedL H Thompson
Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, L 452, P O Box 808, Livermore, CA 94551 0808, USA
Mutat Res 459:1-18. 2000
- Pathways for mitotic homologous recombination in mammalian cellsThomas Helleday
Department of Genetic and Cellular Toxicology, Arrhenius Laboratory, Stockholm University, S 106 91 Stockholm, Sweden
Mutat Res 532:103-15. 2003..In this review, the substrates for HR, the pathways of repair, and their end products (i.e. sister chromatid exchange (SCE), gene conversion, deletions or tandem duplications) are discussed...
- Elevation of sister chromatid exchange in Saccharomyces cerevisiae sgs1 disruptants and the relevance of the disruptants as a system to evaluate mutations in Bloom's syndrome geneF Onoda
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba, Japan
Mutat Res 459:203-9. 2000..In this study, we found that sister chromatid exchange was increased in sgs1 disruptants...
- Alternative lengthening of telomeres is characterized by high rates of telomeric exchangeJ Arturo Londono-Vallejo
Institut National de la Sante et de la Recherche Medicale, Paris, France
Cancer Res 64:2324-7. 2004..These exchanges are telomere specific because there was no correlation with sister chromatid exchange rates at interstitial locations, and they were not observed in non-ALT Bloom syndrome cells with very ..
- UV but not X rays stimulate homologous recombination between sister chromatids and homologs in a Saccharomyces cerevisiae mec1 (ATR) hypomorphic mutantMichael Fasullo
Ordway Research Institute, Albany, NY 12208, USA
Mutat Res 648:73-81. 2008..We suggest that although recombinational repair of double-stand breaks is less efficient in mec1 mutants, recombinants may be generated by other mechanisms, such as break-induced replication...
- BLAP75/RMI1 promotes the BLM-dependent dissolution of homologous recombination intermediatesLeonard Wu
Cancer Research UK, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
Proc Natl Acad Sci U S A 103:4068-73. 2006..Implications of the conserved ability of type IA topoisomerases to catalyze dissolution and how the evolution of factors such as BLAP75/RMI1 might confer specificity on the execution of this process are discussed...
- FANCC suppresses short telomere-initiated telomere sister chromatid exchangeDavid B Rhee
Laboratory of Molecular Gerontology, NIH Biomedical Research Center, National Institute on Aging, Baltimore, MD 21224 6825, USA
Hum Mol Genet 19:879-87. 2010..or Tert(-/-)) with short telomeres, Fancc deficiency led to an increase in the incidence of telomere sister chromatid exchange. In contrast, these phenotypes were not observed in Tert mutant mice with long telomeres...
- Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2Silke Hauf
Research Institute of Molecular Pathology, Vienna, Austria
PLoS Biol 3:e69. 2005..The similarity of the phenotype obtained after expression of nonphosphorylatable SA2 in human cells to that seen after the depletion of Plk1 suggests that SA2 is the critical target of Plk1 in the cohesin dissociation pathway...
- An increase in telomere sister chromatid exchange in murine embryonic stem cells possessing critically shortened telomeresYisong Wang
Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 6445, USA
Proc Natl Acad Sci U S A 102:10256-60. 2005..We have explored the possibility that telomeres may be maintained through telomere sister chromatid exchange (T-SCE) in murine telomere reverse transcriptase-deficient (mTert-/-) splenocytes and ES cells...
- Induction of micronuclei and sister chromatid exchange in bone-marrow cells and abnormalities in sperm of Algerian mice (Mus spretus) exposed to cadmium, lead and zincJoaquim Torres Tapisso
Centro de Biologia Ambiental and Departamento de Biologia Animal, Faculdade de Ciencias da Universidade de Lisboa, Edificio C2, 3 degrees piso, Campo Grande, 1749 016 Lisboa, Portugal
Mutat Res 678:59-64. 2009..Three cytogenetic end points were considered: the frequencies of micronucleated cells (MN) and sister chromatid exchange (SCE) in the bone marrow and the frequency of sperm abnormalities...
- Timeless Maintains Genomic Stability and Suppresses Sister Chromatid Exchange during Unperturbed DNA ReplicationKaren A Urtishak
Abramson Family Cancer Research Institute, Department of Cancer Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
J Biol Chem 284:8777-85. 2009..and Rad51 and Rad52 foci formation selectively during DNA synthesis and caused a 3-4-fold increase in sister chromatid exchange. The sister chromatid exchange events stimulated by Tim reduction were largely mediated via a Brca2/..
- Identification of RecQL1 as a Holliday junction processing enzyme in human cell linesGary LeRoy
Department of Molecular Biology, Princeton University, Washington Road, Princeton, NJ 08544 1014, USA
Nucleic Acids Res 33:6251-7. 2005..A reduction in the level of RecQL1 induced by RNA interference in HeLa cells leads to an increase in sister chromatid exchange. We propose that RecQL1 is involved in the processing of Holliday junctions in human cells.
- Mechanisms of Genetic Reversion in Ichthyosis With ConfettiKeith A Choate; Fiscal Year: 2013..We will examine HR via assays of DNA double strand break (DSB) rates, sister chromatid exchange, and LOH rates and mechanisms in the presence and absence of X-irradiation, a potent inducer of DSB. 2...
- Genomic Stability and RecQ DNA Helicases in YeastSteven J Brill; Fiscal Year: 2012..BLM acts together with DNA topoisomerase III and a newly-identified subunit, Rmi1, to suppress sister chromatid exchange. The fundamental nature of this complex is underscored by its conservation in lower eukaryotes such as ..
- Analysis of replication fork-collapse in yeast by site-specific DNA nickingRANJITH PRASAD ANAND; Fiscal Year: 2013..The potential for fork-collapse to trigger HR will be investigated by an unequal sister chromatid exchange (uSCE) assay...
- Technology for detection and quantitation of telomeric DNA aberrations in cancerHarold C Riethman; Fiscal Year: 2012..and other DNA replication stress, oxidative damage, and homologous recombination events such as unequal sister chromatid exchange (SCE) and T-loop recombination...
- The role of histone chaperone Asf1 in Alternative Lengthening of TelomeresJan Karlseder; Fiscal Year: 2013..regulation all characteristics of ALT emerge in primary and transformed cells, which include telomere sister chromatid exchange, telomere length heterogeneity, the formation of single stranded telomeric C-circles and the ..
- Analysis of Break-Induced ReplicationJames E Haber; Fiscal Year: 2013..Sister-chromatid repair and unequal sister chromatid exchange will be tested, genetically and by real-time DNA analysis...
- CELLULAR AND MOLECULAR RESPONSE TO DNA REPAIR DEFICIENCYRenee Reijo Pera; Fiscal Year: 2000..is hypersensitive to many mutagenic agents, and demonstrates a 10-fold increase in baseline levels of sister chromatid exchange over wild type cells. In addition, EM9 cells are deficient in rejoining DNA single-strand breaks...
- PHENYTOIN PATHOGENESIS OF GINGIVAL OVERGROWTHThomas Hassell; Fiscal Year: 1990..Cytogenetic analyses (e.g., chromosome breakage, sister chromatid exchange) will be performed on fibroblasts from overgrown gingiva, with and without in vitro exposure to the drug...
- BIOLOGICAL MARKERS IN CIS DDP TREATED TESTICULAR CANCERFrederica Perera; Fiscal Year: 1990..cis-DDP, as well as somatic cell mutation (glycophorin A locus) and several generic cytogenetic markers (sister chromatid exchange, micronuclei in lymphocytes, and chromosomal aberrations) attributable to cis- DDP combination therapy...
- DNA Polymerase in Genome Maintenance and TumorigenesisRichard Wood; Fiscal Year: 2006..a range of DNA damaging agents, gross chromosomal arrangements by spectral karyotyping, and frequency of sister chromatid exchange. The results will indicate the most important functions of mammalian Pol zeta with respect to DNA ..
- Analysis of regulation and function of the BLM helicaseNathan Ellis; Fiscal Year: 2003..instability that includes both chromosome breakage and excessive chromatid exchange, in particular, sister chromatid exchange (SCE)...
- EMBRYOS FOR DETECTING ENVIRONMENTAL MUTAGENSStephen Bloom; Fiscal Year: 1992The sister chromatid exchange (SCE) technique has been proposed as a mutagenic assay in genetic toxicology testing...
- RESPIRATORY TRACT IRRITANTS; MECHANISMS AND TOLERANCEYves Alarie; Fiscal Year: 1980..the results, predictions for safe levels of exposure are made for each chemical and evaluation of the effect of these levels is done using sister chromatid exchange frequencies in alveolar macrophages after several days of exposures.
- Manipulating the zebrafish genome through modulation of conserved helicasesShannon Fisher; Fiscal Year: 2007..Reduction of their normal activities leads to an increase in spontaneous sister chromatid exchange and homologue recombination, and also correlates with increased frequency of extrachromosomal homologous ..
- GENOTOXICITY OF GLUTARALDEHYDE--SAFE USE IN DENTISTRYANN DUNIPACE; Fiscal Year: 1991..These tests include: (1) the Ames Salmonella test; (2) an in vitro Chinese hamster ovary cell sister chromatid exchange assay; (3) an a in vivo Chinese hamster bone marrow cell sister chromatid exchange assay; (4) a mouse ..
- FUNCTION OF THE TERMINUS REGION OF THE CHROMOSOMEPETER KUEMPEL; Fiscal Year: 2000..3) Develop physical assays for sister chromatid exchange. This will be used to determine the frequency at which daughter chromosomes interact, how this varies at ..
- CELLULAR GENETIC ASPECTS OF OPIATE USEArthur Falek; Fiscal Year: 1993..in their ability to repair far UV damage as measured by unscheduled DNA synthesis and an alteration in sister chromatid exchange response when stressed by UV and mitomycin C...
- Repair Pathways for Ionizing Radiation Damage YeastJohn Game; Fiscal Year: 2006..Recent and existing mutants will be studied in detail with this system to determine the molecular involvement of each gene in DSB repair, sister chromatid exchange and recombination between chromosomes.
- MOLECULAR STUDIES OF HUMAN GENETIC RECOMBINATIONM Meyn; Fiscal Year: 1990..measure extrachromosomal recombination, intrachromosomal recombination, interchromosomal recombination (sister chromatid exchange) and gene conversion in normal fibroblast lines and fibroblast lines derived from patients with ..
- GENETIC ANALYSIS OF THE EUKARYOTIC CELL CYCLELeland Hartwell; Fiscal Year: 2000..5) Elucidate the pathways of sister chromatid exchange which occur during cell cycle arrest at the RAD9 checkpoint...
- ABERRANT PAPOVAVIRUS REPLICATION AFTER GENOTOXIC DAMAGEROBERT SNAPKA; Fiscal Year: 1991..newly replicated daughter chromosomes in mammalian cells, and provided evidence for the mechanism of sister chromatid exchange. These lines of investigation will be extended and several other aspects of aberrant DNA replication ..
- HUMAN TUMOR CELL RADIOSENSITIVITY VERSUS RADIOCURABILITYWilliam Brock; Fiscal Year: 1992..will also be analyzed for heterogeneity of radiation sensitivity by both a survival assay and the sister chromatid exchange assay...
- Activating liver carcinogens in yeast by expressing CYP450 polymorphismsMichael Fasullo; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- RADIATION INDUCTION OF GENOMIC REARRANGEMENTS IN YEASTMichael Fasullo; Fiscal Year: 2004..abstract_text> ..
- GENOTOXICITY OF BENZIDINE ANALOGUESJOSEPH SINSHEIMER; Fiscal Year: 1992..In vitro mutagenicity and adduct formation results will be compared to in vivo genotoxicity where the end points will be adduct formation and bone marrow genotoxicity, as measured by chromosome aberrations and sister chromatid exchange.
- Homologous Recombination & Human Cell RadiosensitivityLAWRENCE THOMPSON; Fiscal Year: 2009..These integrated studies may lead to a more rational basis for cancer radiotherapy and bring'insights into how HRR prevents the initiation of carcihogenesis by endogenous processes and exogenous agents. ..
- GENETIC ANALYSIS OF NUCLEOTIDE EXCISION REPAIRLAWRENCE THOMPSON; Fiscal Year: 2002..These mechanistic insights will guide future structural studies on ERCC2. ..
- Gene Activation by Remote Transcriptional EnhancersDale Dorsett; Fiscal Year: 2004..This will help illuminate the basic mechanisms underlying gene expression defects that occur in some human diseases. ..
- FANCONI ANEMIA GENE PATHWAY IN RADIATION RESPONSESLAWRENCE THOMPSON; Fiscal Year: 2004..The results of these studies will lead to more specific models of the nature of the FA protein "pathway" and its quantitative contributions to multiple biological effects associated with IR-mediated oxidative damage. ..
- Mechanism of Meiotic Pairing in DrosophilaBRUCE MCKEE; Fiscal Year: 2007..3) What is the molecular basis for homologous pairing in early prophase. 4) How are homologous pairing and conjunction related? We will use predominant genetic and cell biological methods to address these questions. ..
- Base Excision Repair, Genetic Integrity & Health SpanCHRISTI WALTER; Fiscal Year: 2008..Genetic integrity, apoptosis, and pathology will be examined using a variety of molecular, biochemical and cell biological approaches. ..
- Germ Cell AgingCHRISTI WALTER; Fiscal Year: 2007..This study will advance our understanding of the fundamental mechanisms involved in male germline integrity. ..
- Mechanisms of Double-strand Break Repair in YeastLORRAINE SYMINGTON; Fiscal Year: 2009..The last aim includes in vitro studies to identify preferred DNA substrates for Rad55:Rad57 binding and stabilization of the Rad51 nucleoprotein by Rad55:Rad57 in the presence or absence of Srs2. ..
- Genetic Recombination and Chromosome RearrangementsLORRAINE SYMINGTON; Fiscal Year: 2007..Much of the conference will be devoted to topics of concern both to the National Cancer Institute and to the Institute of Environmental and Health Sciences. [unreadable] [unreadable] [unreadable] [unreadable]..
- DNA Damage and Neurodegeneration in Cockayne SyndromeJames Cleaver; Fiscal Year: 2009..Successful conclusion of these studies will expand our knowledge of mechanisms of neurodegeneration and lay groundwork for development of therapeutic approaches for CS patients. ..
- Identifying Mechanisms for Chromosome 21 NondisjunctionTiffany Oliver; Fiscal Year: 2007..We will use a modified tetrad analysis to infer the exchange pattern from the observed recombination data. [unreadable] [unreadable] [unreadable]..
- The Function of SWAP-70 in Mast CellsRolf Jessberger; Fiscal Year: 2007..The proposed experiments should yield key information on pathways regulating mast cell exocytosis, and will help understanding mast cell-associated diseases such as allergic reactions. ..
- GENETIC/ CYTOGENETIC INSTABILITY OF IONIZING RADIATIONMichael Cornforth; Fiscal Year: 2002....
- RADIOLOGICAL RESEARCH ACCELERATOR FACILITY (RARAF)David Brenner; Fiscal Year: 2002..abstract_text> ..
- High Stability Accelerator for Sub-Cellular MicrobeamDavid Brenner; Fiscal Year: 2003..We have considerably more demand for microbeam time than we can cope with, primarily because of limited physicist manpower. ..
- Novel approaches in linkage analysis for complex traitsMariza de Andrade; Fiscal Year: 2003..No new data will be collected for this grant proposal. The application clearly fits the goal of this NHLBI grant program since it will explore new approaches using an existing data set collected through NHLBI support. ..
- Genetic Recombination/Genomic Rearrangements ConferenceLORRAINE SYMINGTON; Fiscal Year: 2005..abstract_text> ..
- The XP Variant: A Human Mutator Gene for UV DamageJames Cleaver; Fiscal Year: 2005..We will express hRad3O on the keratin 14 promoter for over-expression in the skin, and make targeted knockout of mRad3O in vivo to identify roles for hRad3O in promoting and preventing carcinogenesis. ..
- Cancer Risks Attributable To Radiation from Pediatric CTDavid Brenner; Fiscal Year: 2006..The target audiences will be radiologists, pediatricians, family physicians, and the CT industry. We will use telephone interviews supplemented with survey questions, to aid in web content decisions. ..
- Functions of SWAP-70 in B Cell ActivationRolf Jessberger; Fiscal Year: 2006..Therefore, the analysis of SWAP-70 in B cell activation is likely to help in our understanding of normal B cell function as well as of immune deficiencies, B cell malignancies, autoimmunity, and allergy. ..
- Activation & Proliferation of Hematopoietic Stem CellsPeter Lansdorp; Fiscal Year: 2006..Such information is relevant for a basic understanding of hematopoiesis as well as applications of stem cells in and outside hematology. ..
- RECOMBINATION MECHANISMS IN YEAST CELL DIFFERENTIATIONJames Haber; Fiscal Year: 2006..abstract_text> ..
- MECHANISMS OF MICROTUBULE AND MITOTIC SPINDLE ASSEMBLYLynne Cassimeris; Fiscal Year: 2006..To address these issues, we will apply a number of fluorescence-based imaging methods combined with siRNA or injection of function-blocking proteins or antibodies. ..
- GERM CELLS--DNA REPAIR, MUTATION, & ENVIRONMENTAL AGENTSCHRISTI WALTER; Fiscal Year: 2001..This study will advance an understanding of the fundamental mechanisms involved in mutagenesis in germ cells. ..