genetic transformation

Summary

Summary: Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.

Top Publications

  1. ncbi Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation
    Tsuyoshi Nakagawa
    Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, 1060 Nishikawatsu, Matsue, Japan
    J Biosci Bioeng 104:34-41. 2007
  2. ncbi The genetic transformation machinery: composition, localization, and mechanism
    Jean Pierre Claverys
    Centre National de la Recherche Scientifique, LMGM UMR, Toulouse, France
    FEMS Microbiol Rev 33:643-56. 2009
  3. pmc An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae
    L S Havarstein
    Laboratory of Microbial Gene Technology, Agricultural University of Norway, As, Norway
    Proc Natl Acad Sci U S A 92:11140-4. 1995
  4. ncbi pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation
    R P Hellens
    John Innes Centre, Norwich Research Park, Colney, UK
    Plant Mol Biol 42:819-32. 2000
  5. pmc Competence for genetic transformation in encapsulated strains of Streptococcus pneumoniae: two allelic variants of the peptide pheromone
    G Pozzi
    Sezione di Microbiologia, Dipartimento di Scienze Chirurgiche, Universitá di Cagliara, Italy
    J Bacteriol 178:6087-90. 1996
  6. pmc Identification of a new regulator in Streptococcus pneumoniae linking quorum sensing to competence for genetic transformation
    M S Lee
    Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois 60607, USA
    J Bacteriol 181:5004-16. 1999
  7. ncbi High efficiency transfection of Plasmodium berghei facilitates novel selection procedures
    Chris J Janse
    Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
    Mol Biochem Parasitol 145:60-70. 2006
  8. pmc Genetic transformation of a Corynebacterial symbiont from the Chagas disease vector Triatoma infestans
    Ravi V Durvasula
    Department of Internal Medicine, University of New Mexico, 915 Camino Del Salud NE, CRF 305, Albuquerque, NM 87131, USA
    Exp Parasitol 119:94-8. 2008
  9. ncbi A Streptomyces rimosus aphVIII gene coding for a new type phosphotransferase provides stable antibiotic resistance to Chlamydomonas reinhardtii
    I Sizova
    Biological Institute, St Petersburg State University, Oranienbaumskoye sch, 2, St Petersburg, 198904 Russia
    Gene 277:221-9. 2001
  10. ncbi Construct design for efficient, effective and high-throughput gene silencing in plants
    S V Wesley
    CSIRO Plant Industry, PO Box 1600 Canberra ACT 2601, Australia
    Plant J 27:581-90. 2001

Detail Information

Publications347 found, 100 shown here

  1. ncbi Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation
    Tsuyoshi Nakagawa
    Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, 1060 Nishikawatsu, Matsue, Japan
    J Biosci Bioeng 104:34-41. 2007
    ..Moreover, tissue-specific and inducible gene expression using a promoter was also monitored with pGWBs. It is expected that, the pGWB system will serve as a powerful tool for plasmid construction in plant research...
  2. ncbi The genetic transformation machinery: composition, localization, and mechanism
    Jean Pierre Claverys
    Centre National de la Recherche Scientifique, LMGM UMR, Toulouse, France
    FEMS Microbiol Rev 33:643-56. 2009
    Natural genetic transformation is widely distributed in bacteria. It is a genetically programmed process that is inherent to the species...
  3. pmc An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae
    L S Havarstein
    Laboratory of Microbial Gene Technology, Agricultural University of Norway, As, Norway
    Proc Natl Acad Sci U S A 92:11140-4. 1995
    Competence for genetic transformation in Streptococcus pneumoniae has been known for three decades to arise in growing cultures at a critical cell density, in response to a secreted protease-sensitive signal...
  4. ncbi pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation
    R P Hellens
    John Innes Centre, Norwich Research Park, Colney, UK
    Plant Mol Biol 42:819-32. 2000
    ..Information on the pGreen plasmid system is supplemented by an Internet site (http://www.pgreen.ac.uk) through which comprehensive information, protocols, order forms and lists of different pGreen marker gene permutations can be found...
  5. pmc Competence for genetic transformation in encapsulated strains of Streptococcus pneumoniae: two allelic variants of the peptide pheromone
    G Pozzi
    Sezione di Microbiologia, Dipartimento di Scienze Chirurgiche, Universitá di Cagliara, Italy
    J Bacteriol 178:6087-90. 1996
    ..Both CSP-1 and CSP-2 synthetic peptides were used to induce competence in the 42 strains; 48% of the strains became competent after the addition of the synthetic peptide, whereas none were transformable without the added peptides...
  6. pmc Identification of a new regulator in Streptococcus pneumoniae linking quorum sensing to competence for genetic transformation
    M S Lee
    Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois 60607, USA
    J Bacteriol 181:5004-16. 1999
    Competence for genetic transformation in Streptococcus pneumoniae is regulated by a quorum-sensing system encoded by two genetic loci, comCDE and comAB...
  7. ncbi High efficiency transfection of Plasmodium berghei facilitates novel selection procedures
    Chris J Janse
    Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
    Mol Biochem Parasitol 145:60-70. 2006
    ..The combination of enhanced transfection efficiency and a versatile rodent model provides a basis for the further development of novel tools for high throughput genome manipulation...
  8. pmc Genetic transformation of a Corynebacterial symbiont from the Chagas disease vector Triatoma infestans
    Ravi V Durvasula
    Department of Internal Medicine, University of New Mexico, 915 Camino Del Salud NE, CRF 305, Albuquerque, NM 87131, USA
    Exp Parasitol 119:94-8. 2008
    ..Emergence of resistance to pesticides greatly hampers control efforts. Paratransgenesis, or the genetic transformation of bacterial symbionts of disease vectors, is an alternative to traditional approaches...
  9. ncbi A Streptomyces rimosus aphVIII gene coding for a new type phosphotransferase provides stable antibiotic resistance to Chlamydomonas reinhardtii
    I Sizova
    Biological Institute, St Petersburg State University, Oranienbaumskoye sch, 2, St Petersburg, 198904 Russia
    Gene 277:221-9. 2001
    ..We conclude that the aphVIII gene is a useful marker for nuclear transformation and promoter tagging of C. reinhardtii wild-type and probably other green algae...
  10. ncbi Construct design for efficient, effective and high-throughput gene silencing in plants
    S V Wesley
    CSIRO Plant Industry, PO Box 1600 Canberra ACT 2601, Australia
    Plant J 27:581-90. 2001
    ..This system may facilitate the large-scale determination and discovery of plant gene functions in the same way as RNAi is being used to examine gene function in Caenorhabditis elegans...
  11. ncbi Genetic transformation of Drosophila with transposable element vectors
    G M Rubin
    Science 218:348-53. 1982
    ..To demonstrate the generality of this approach, a DNA segment that does not confer a recognizable phenotype on recipients was also transferred into germ line chromosomes...
  12. ncbi A novel genetic system to detect protein-protein interactions
    S Fields
    Department of Microbiology, State University of New York at Stony Brook, Stony Brook 11794
    Nature 340:245-6. 1989
    ..High transcriptional activity is obtained only when both hybrids are present in a cell. This system may be applicable as a general method to identify proteins that interact with a known protein by the use of a simple galactose selection...
  13. pmc Parameters determining the efficiency of gene targeting in the moss Physcomitrella patens
    Yasuko Kamisugi
    Centre for Plant Sciences, Faculty of Biological Sciences, Leeds University, Leeds LS2 9JT, UK
    Nucleic Acids Res 33:e173. 2005
    ..Homologous recombination in Physcomitrella is substantially more efficient than in any multicellular eukaryote, recommending it as the outstanding model for the study of homologous recombination in plants...
  14. ncbi Expression profiling reveals off-target gene regulation by RNAi
    Aimee L Jackson
    Rosetta Inpharmatics LLC, 12040 115th Avenue NE, Kirkland, Washington 98034, USA
    Nat Biotechnol 21:635-7. 2003
    ..These results demonstrate that siRNAs may cross-react with targets of limited sequence similarity...
  15. ncbi A new moss genetics: targeted mutagenesis in Physcomitrella patens
    Didier G Schaefer
    Institut d Ecologie, Laboratoire de Phytogénétique Cellulaire, Batiment de Biologie, Universite de Lausanne, CH 1015 Lausanne, Switzerland
    Annu Rev Plant Biol 53:477-501. 2002
    ..This article reviews our current knowledge of Physcomitrella patens transformation and its unique suitability for functional genomic studies...
  16. ncbi Molecular analysis of the acetolactate synthase gene of Chlamydomonas reinhardtii and development of a genetically engineered gene as a dominant selectable marker for genetic transformation
    Joy L Kovar
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Plant J 29:109-17. 2002
    ..5 x 10-6 transformants/cell using the glass bead vortexing method. These results suggest that pJK7 and pRP-ALS can serve as important additional dominant selectable markers for the genetic transformation of C. reinhardtii.
  17. pmc Competence for genetic transformation in Streptococcus pneumoniae: termination of activity of the alternative sigma factor ComX is independent of proteolysis of ComX and ComW
    Andrew Piotrowski
    Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois 60607, USA
    J Bacteriol 191:3359-66. 2009
    Competence for genetic transformation in Streptococcus pneumoniae is a transient physiological state whose development is coordinated by a peptide pheromone (CSP) and its receptor, which activates transcription of two downstream genes, ..
  18. pmc Activation tagging in Arabidopsis
    D Weigel
    Plant Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Plant Physiol 122:1003-13. 2000
    ..This has important implications for the spectrum of genes that will be discovered by this method...
  19. ncbi Genetic transformation of Saccharomyces cerevisiae and Chlamydomonas reinhardtii mitochondria
    Nathalie Bonnefoy
    Centre de Genetique Moleculaire, CNRS UPR2167, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    Methods Cell Biol 80:525-48. 2007
  20. ncbi A putative Ca2+ and calmodulin-dependent protein kinase required for bacterial and fungal symbioses
    Julien Levy
    Laboratoire des Interactions Plantes Microorganismes INRA CNRS, BP27, 31326 Castanet Tolosan Cedex, France
    Science 303:1361-4. 2004
    ..This indicates that calcium spiking is likely an essential component of the signaling cascade leading to nodule development and mycorrhizal infection, and sheds light on the biological role of plant CCaMKs...
  21. ncbi A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques
    S Travella
    John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK
    Plant Cell Rep 23:780-9. 2005
    ..For the efficient production of transgenic barley plants, with stable transgene expression and reduced silencing, the Agrobacterium-mediated method appears to offer significant advantages over particle bombardment...
  22. ncbi Novel mutagenic properties of abasic sites in Saccharomyces cerevisiae
    P E Gibbs
    Department of Biophysics, University of Rochester School of Medicine and Dentistry, NY 14642, USA
    J Mol Biol 251:229-36. 1995
    ..Predominant dCMP insertion was observed even when template guanine bases were excluded from a region extending 19 nucleotides 5', and 13 nucleotides 3', to the abasic site...
  23. ncbi A reporter gene system for the hyperthermophilic archaeon Sulfolobus solfataricus based on a selectable and integrative shuttle vector
    Melanie Jonuscheit
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstr 10, Germany
    Mol Microbiol 48:1241-52. 2003
    ....
  24. ncbi pORE: a modular binary vector series suited for both monocot and dicot plant transformation
    Catherine Coutu
    Agriculture and Agrifood Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, Canada S7N 0X2
    Transgenic Res 16:771-81. 2007
    ..The sets comprise various combinations of promoters (P (HPL), P (ENTCUP2) and P (TAPADH)), selectable markers (nptII and pat) and reporter genes (gusA and smgfp)...
  25. pmc High-level production of beta-carotene in Saccharomyces cerevisiae by successive transformation with carotenogenic genes from Xanthophyllomyces dendrorhous
    René Verwaal
    Fungal Genomics, Laboratory of Microbiology, Wageningen University, Dreijenlaan 2, Wageningen, The Netherlands
    Appl Environ Microbiol 73:4342-50. 2007
    ..This transformant is promising for further development toward the biotechnological production of beta-carotene by S. cerevisiae...
  26. ncbi Functional transient genetic transformation of Arabidopsis leaves by biolistic bombardment
    Shoko Ueki
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794 5215, USA
    Nat Protoc 4:71-7. 2009
    ..Using this procedure, the data are obtained after 2-4 d of work...
  27. ncbi RNA silencing genes control de novo DNA methylation
    Simon W L Chan
    Department of MCD Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA
    Science 303:1336. 2004
  28. ncbi Agrobacterium rhizogenes-mediated transformation of soybean to study root biology
    Attila Kereszt
    ARC Centre of Excellence for Integrative Legume Research, The University of Queensland, St Lucia, Queensland 4072, Australia
    Nat Protoc 2:948-52. 2007
    ..Using this method, almost 100% of the infected plants form hairy roots within 1 month from the start of the experiments...
  29. ncbi Agrobacterium rhizogenes-transformed roots of Medicago truncatula for the study of nitrogen-fixing and endomycorrhizal symbiotic associations
    A Boisson-Dernier
    , INRA-CNRS UMR215, Castanet-Tolosan, France
    Mol Plant Microbe Interact 14:695-700. 2001
    ..Our results suggest that M. truncatula hairy roots represent a particularly attractive system with which to study endosymbiotic associations in transgenically modified roots...
  30. ncbi Sub1A is an ethylene-response-factor-like gene that confers submergence tolerance to rice
    Kenong Xu
    Department of Plant Pathology, University of California, Davis, California 95616, USA
    Nature 442:705-8. 2006
    ..Cultivation of this variety is expected to provide protection against damaging floods and increase crop security for farmers...
  31. pmc Uncoupling of the functions of the Arabidopsis VIP1 protein in transient and stable plant genetic transformation by Agrobacterium
    Jianxiong Li
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Proc Natl Acad Sci U S A 102:5733-8. 2005
    Agrobacterium-mediated genetic transformation of plants, a unique example of transkingdom DNA transfer, requires the presence of several proteins encoded by the host cell...
  32. ncbi An alpha-(1,4)-amylase is essential for alpha-(1,3)-glucan production and virulence in Histoplasma capsulatum
    Christopher L Marion
    Department of Molecular Microbiology, Washington University, St Louis, MO 63110, USA
    Mol Microbiol 62:970-83. 2006
    ..Loss of AMY1 function attenuated the ability of Histoplasma to kill macrophages and to colonize murine lungs...
  33. ncbi Genetic transformation of mature citrus plants
    Magdalena Cervera
    Department of Plant Protection and Biotechnology, Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain
    Methods Mol Biol 286:177-88. 2005
    ..Because of a decline in regenerative and transformation potential, genetic transformation procedures usually employ juvenile material as the source of plant tissue, therefore resulting in the ..
  34. pmc Genetic transformation of the Lyme disease agent Borrelia burgdorferi with coumarin-resistant gyrB
    D S Samuels
    Laboratory of Vectors and Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840
    J Bacteriol 176:6045-9. 1994
    ..burgdorferi. The coumermycin A1-resistant gyrB marker and genetic transformation can now be applied toward dissecting the physiology and pathogenesis of the Lyme disease agent on a ..
  35. pmc The relative roles in vivo of Saccharomyces cerevisiae Pol eta, Pol zeta, Rev1 protein and Pol32 in the bypass and mutation induction of an abasic site, T-T (6-4) photoadduct and T-T cis-syn cyclobutane dimer
    Peter E M Gibbs
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    Genetics 169:575-82. 2005
    ..In contrast, bypass of the T-T dimer in the pol32delta strain occurs at the wild-type frequency...
  36. ncbi Identification of ComW as a new component in the regulation of genetic transformation in Streptococcus pneumoniae
    Ping Luo
    Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA
    Mol Microbiol 54:172-83. 2004
    Regulation of competence for genetic transformation in Streptococcus pneumoniae depends on a quorum-sensing system, genes involved in DNA uptake and recombination and a link between these two gene sets...
  37. ncbi The moss Physcomitrella patens
    David Cove
    Center for Plant Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom
    Annu Rev Genet 39:339-58. 2005
    ..Over 100,000 ESTs have been sequenced and annotated, and sequencing of the genome should be completed by the end of 2005...
  38. ncbi Genetic transformation and gene silencing mediated by multiple copies of a transgene in eastern white pine
    Wei Tang
    Department of Biology, Howell Science Complex, East Carolina University, Greenville, NC 27858 4353, USA
    J Exp Bot 58:545-54. 2007
    ..There were no differences in shoot differentiation and development between transgenic lines with multiple T-DNA copies and transgenic lines with one or two T-DNA copies...
  39. pmc The recognition and modification sites for the bacterial type I restriction systems KpnAI, StySEAI, StySENI and StySGI
    Julie K A Kasarjian
    Division of Microbiology and Molecular Genetics, Department of Biochemistry and Microbiology, Loma Linda University, Loma Linda, CA 92350, USA
    Nucleic Acids Res 32:e82. 2004
    ..For confirmation, oligonucleotides containing each of the predicted sequences were synthesized, cloned into plasmid pMECA and transformed into each strain, resulting in a large reduction in efficiency of transformation (EOT)...
  40. pmc New restriction enzymes discovered from Escherichia coli clinical strains using a plasmid transformation method
    Julie K A Kasarjian
    Division of Microbiology and Molecular Genetics, Department of Biochemistry and Microbiology, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA
    Nucleic Acids Res 31:e22. 2003
    ..Eco1158I, an isoschizomer of EcoBI, was also found in this study...
  41. pmc piggybac- and PhiC31-mediated genetic transformation of the Asian tiger mosquito, Aedes albopictus (Skuse)
    Geneviève M C Labbé
    Oxitec Limited, Oxford, United Kingdom
    PLoS Negl Trop Dis 4:e788. 2010
    ..In the absence of vaccine or antiviral drugs, efficient mosquito control strategies are crucial. Conventional control methods have so far failed to control Ae. albopictus adequately...
  42. ncbi Agrobacterium tumefaciens-mediated transformation of the plant pathogenic fungus, Magnaporthe grisea
    H S Rho
    School of Agricultural Biotechnology and Research Center for New Bio-Materials in Agriculture, Seoul National University, Suwon, Korea
    Mol Cells 12:407-11. 2001
    ..Considering the efficiency and flexibility of A. tumefaciens-mediated transformation (ATMT), this technique offers highly efficient means for characterizing the genes that are important for the pathogenicity of M. grisea...
  43. ncbi Recent advances in the genetic transformation of trees
    L Pena
    Dept of Plant Protection and Biotechnology, Instituto Valenciano de Investigaciones Agrarias IVIA, Apartado Oficial, 46113 Moncada, Valencia, Spain
    Trends Biotechnol 19:500-6. 2001
    ..ago, trees were considered to be recalcitrant material for most molecular biology techniques, including genetic transformation. However, transgenes for shortening the juvenile phase or for phytoremediation purposes have now been ..
  44. ncbi Genetic transformation of cowpea (Vigna unguiculata L.) and stable transmission of the transgenes to progeny
    J Carlos Popelka
    CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia
    Plant Cell Rep 25:304-12. 2006
    ..These results pave the way for the introduction of new traits into cowpea and the first genes to be trialled will include those with potential to protect against insect pests...
  45. ncbi A case of promiscuity: Agrobacterium's endless hunt for new partners
    Benoît Lacroix
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Trends Genet 22:29-37. 2006
    ..we present Agrobacterium-mediated transformation of non-plant organisms as a source of new protocols for genetic transformation, as a unique tool for genomic studies (insertional mutagenesis or targeted DNA integration) and as a ..
  46. pmc Cloning of human centromeres by transformation-associated recombination in yeast and generation of functional human artificial chromosomes
    N Kouprina
    Laboratory of Biosystems and Cancer, Center for Cancer Research, National Cancer Institute, NIH, Building 37, Room 5032, Bethesda, MD 20892, USA
    Nucleic Acids Res 31:922-34. 2003
    ..In summary, our results demonstrated that TAR cloning is a useful tool for investigating human centromere organization and the structural requirements for formation of HAC vectors that might have a potential for therapeutic applications...
  47. ncbi A novel carbon source-dependent genetic transformation system for the versatile cell factory Hypocrea jecorina (anamorph Trichoderma reesei)
    Zhang Guangtao
    State Key Laboratory of Microbial Technology, Shandong University, Jinan Shandong, China
    FEMS Microbiol Lett 303:26-32. 2010
    b>Genetic transformation is an indispensable tool for basic fungal research, as well as a useful technique for directed improvement of industrial strains...
  48. ncbi Transformation of Stomoxys calcitrans with a Hermes gene vector
    D A O'Brochta
    Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, College Park, MD 20742 4450, USA
    Insect Mol Biol 9:531-8. 2000
    ..melanogaster should be considered for widespread, constitutive expression. All transgenic lines contained multiple (2-4) integrated Hermes elements. Hermes integration events occurred through a canonical cut-and-paste mechanism...
  49. pmc Genetic transformation of cotton with a harpin-encoding gene hpaXoo confers an enhanced defense response against different pathogens through a priming mechanism
    Weiguo Miao
    Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China
    BMC Plant Biol 10:67. 2010
    ..To date, most upland cotton varieties are susceptible to V. dahliae and the breeding for cotton varieties with the resistance to Verticillium wilt has not been successful...
  50. ncbi Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplast
    Meng Sun
    Department of Biotechnology, College of Life Science, Zhejiang University, Hangzhou 310027, PR China
    Biotechnol Lett 25:1087-92. 2003
    ..These experimental results support the possibility of using transgenic chloroplasts of green alga as a mucosal vaccine source...
  51. pmc Development of a Saccharomyces cerevisiae strain with enhanced resistance to phenolic fermentation inhibitors in lignocellulose hydrolysates by heterologous expression of laccase
    S Larsson
    Department of Applied Microbiology, Lund University, SE-221 00 Lund, Sweden
    Appl Environ Microbiol 67:1163-70. 2001
    ..Our results demonstrate the importance of phenolic compounds as fermentation inhibitors and the advantage of using laccase-expressing yeast strains for producing ethanol from lignocellulose...
  52. ncbi Highly efficient stable transformation of bloodstream forms of Trypanosoma brucei
    Gabriela Burkard
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
    Mol Biochem Parasitol 153:220-3. 2007
  53. ncbi Genetic transformation of yeast
    R D Gietz
    Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Ave, Winnipeg, MB, Canada, R3M 0W3
    Biotechniques 30:816-20, 822-6, 828 passim. 2001
    b>Genetic transformation was first described by Griffith in 1928 and has since been demonstrated in a variety of organisms, including many species of fungi...
  54. pmc Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize
    Changfu Zhu
    Departament de Produccio Vegetal i Ciencia Forestal, Universitat de Lleida, Avenue Alcalde Rovira Roure, 191, 25198 Lleida, Spain
    Proc Natl Acad Sci U S A 105:18232-7. 2008
    ..Combinatorial transformation is a versatile approach that could be used to modify any metabolic pathway and pathways controlling other biochemical, physiological, or developmental processes...
  55. ncbi piggyBac internal sequences are necessary for efficient transformation of target genomes
    X Li
    Department of Biological Sciences, and Center for Tropical Diseases Research and Training, University of Notre Dame, Notre Dame, IN 46556, USA
    Insect Mol Biol 14:17-30. 2005
    ..Sequencing analyses of our constructs verify the position and the source of a point mutation within the 3' internal repeat sequence of our vectors that has no apparent effect on transformation efficiency...
  56. ncbi Genetic transformation of barley (Hordeum vulgare L.) via infection of androgenetic pollen cultures with Agrobacterium tumefaciens
    Jochen Kumlehn
    Institute of Plant Genetics and Crop Plant Research Gatersleben, Plant Reproductive Biology, Corrensstr 3, 06466 Gatersleben, Germany
    Plant Biotechnol J 4:251-61. 2006
    A novel genetic transformation method for barley (Hordeum vulgare L.), based on infection of androgenetic pollen cultures with Agrobacterium tumefaciens, is presented. Winter-type barley cv...
  57. ncbi Malaria control with genetically manipulated insect vectors
    Luke Alphey
    Oxford University, UK
    Science 298:119-21. 2002
    ..At a recent workshop, experts discussed the benefits, risks, and research priorities associated with using genetically manipulated insects in the control of vector-borne diseases...
  58. ncbi Involvement of targeted proteolysis in plant genetic transformation by Agrobacterium
    Tzvi Tzfira
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794 5215, USA
    Nature 431:87-92. 2004
    b>Genetic transformation of plant cells by Agrobacterium represents a unique case of trans-kingdom DNA transfer...
  59. ncbi High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens
    Y Ishida
    Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc, Shizuoka, Japan
    Nat Biotechnol 14:745-50. 1996
    ..F1 hybrids between A188 and five other inbreds were transformed at low frequencies...
  60. ncbi Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus
    Maria Cecilia Zanek
    Facultad de Ciencias Exactas, Instituto de Bioquímica y Biología Molecular IBBM, U N L P, Calles 47 y 115, 1900, La Plata, Argentina
    Plant Cell Rep 27:57-66. 2008
    ..The ORF was cytosine methylated suggesting a PTGS mechanism, although the transformant failed to protect against the viral load used. Possible causes for the failed protection against the CPsV are discussed...
  61. ncbi Gene targeting in Physcomitrella patens
    D G Schaefer
    Institut d Ecologie, Laboratoire de Phytogénétique Cellulaire, Batiment de Biologie, Universite de Lausanne, CH 1015, Lausanne, Switzerland
    Curr Opin Plant Biol 4:143-50. 2001
    ..Physcomitrella patens, as the new 'green yeast', might well become a major tool for functional genomic studies of multicellular eukaryotes...
  62. pmc Natural genetic transformation: A novel tool for efficient genetic engineering of the dairy bacterium Streptococcus thermophilus
    Trinelise Blomqvist
    Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, PO Box 5003, 1432 As, Norway
    Appl Environ Microbiol 72:6751-6. 2006
    ..The efficiency of this novel tool should make it possible to construct food-grade mutants of S. thermophilus, opening up exciting new possibilities that should benefit consumers as well as the dairy industry...
  63. ncbi Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis
    P K Burkhardt
    Swiss Federal Institute of Technology, Institute for Plant Sciences, Zurich, Switzerland
    Plant J 11:1071-8. 1997
    ..These results have important implications for long-term prospects of overcoming worldwide vitamin A deficiency...
  64. ncbi Karyotype analysis, genome organization, and stable genetic transformation of the root colonizing fungus Piriformospora indica
    Alga Zuccaro
    Justus Liebig University Giessen, Institute of Phytopathology and Applied Zoology IPAZ, Giessen, Germany
    Fungal Genet Biol 46:543-50. 2009
    ..Here we provide data on the karyotype and genetic transformation strategies. The fungus was shown to possess at least six chromosomes and a genome size of about 15.4-24Mb...
  65. ncbi Highly sensitive, fluorescent transformation marker for Drosophila transgenesis
    C Horn
    Lehrstuhl fur Genetik, Universitat Bayreuth, Universitätsstrasse 30 NWI, 95447 Bayreuth, Germany
    Dev Genes Evol 210:623-9. 2000
    ..This will be of great interest to the field of evolutionary developmental biology and to modern pest management programs...
  66. pmc ComEA, a Bacillus subtilis integral membrane protein required for genetic transformation, is needed for both DNA binding and transport
    G S Inamine
    Public Health Research Institute, New York, New York 10016, USA
    J Bacteriol 177:3045-51. 1995
    ..This C-terminal domain possesses a sequence with similarity to those of several proteins known to be involved in nucleic acid transactions including UvrC and a human protein that binds to the replication origin of the Epstein-Barr virus...
  67. ncbi Genetic transformation of NERICA, interspecific hybrid rice between Oryza glaberrima and O. sativa, mediated by Agrobacterium tumefaciens
    Takuma Ishizaki
    Biological Resources Division, Japan International Research Center for Agricultural Sciences, 1 1 Ohwashi, Tsukuba, Ibaraki 305 8686, Japan
    Plant Cell Rep 27:319-27. 2008
    ..We also obtained transformed plants by inoculation of calluses derived from mature seeds, but the frequency of transformation was lower and sterility was more frequent...
  68. ncbi Characterization of T-DNA insertion sites in Arabidopsis thaliana and the implications for saturation mutagenesis
    Patrick J Krysan
    Horticulture Department, University of Wisconsin Madison 53706, USA
    OMICS 6:163-74. 2002
    ..However, this bias does not limit the utility of T-DNA as an effective insertional mutagen for use in reverse-genetic strategies...
  69. pmc Gene discovery and gene function assignment in filamentous fungi
    L Hamer
    Paradigm Genetics, 108 Alexander Drive, Research Triangle Park, NC 27709, USA
    Proc Natl Acad Sci U S A 98:5110-5. 2001
    ..We further show that insertions can create alterations in gene expression, and we have used this approach to investigate an amino acid oxidation pathway in two important fungal phytopathogens...
  70. pmc Chloroplast vector systems for biotechnology applications
    Dheeraj Verma
    Department of Molecular Biology and Microbiology, College of Medicine, University of Central Florida, Orlando, Florida 32816 2364, USA
    Plant Physiol 145:1129-43. 2007
  71. ncbi MicroTom--a high-throughput model transformation system for functional genomics
    Yinghui Dan
    Monsanto Company, 700 Chesterfield Parkway, Chesterfield, MO 63017, USA
    Plant Cell Rep 25:432-41. 2006
    ..The utility of this MicroTom model transformation system for functional genomic studies, such as identification of genes related to important agricultural traits and gene function, is discussed...
  72. ncbi Progress towards the development of a transgenic strain of the Australian sheep blowfly (Lucilia cuprina) suitable for a male-only sterile release program
    Maxwell J Scott
    Institute of Molecular Biosciences, Massey University, Private Bag 11222, Palmerston North, New Zealand
    Insect Biochem Mol Biol 34:185-92. 2004
    ..We anticipate that the female-killing system will need to be optimised for L. cuprina in order to make a strain with the properties required for a male-only release program...
  73. ncbi Genetic transformation of cork oak (Quercus suber L.) for herbicide resistance
    Ruben Alvarez
    Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK
    Biotechnol Lett 31:1477-83. 2009
    ..Molecular and biochemical assays confirmed the stability and expression of the transgenes 3 months after thawing the cultures. These results demonstrate genetic engineering of herbicide tolerance in Quercus spp...
  74. pmc "Parahomologous" gene targeting in Drosophila cells: an efficient, homology-dependent pathway of illegitimate recombination near a target site
    L Cherbas
    Department of Biology, Indiana University, Bloomington 47405, USA
    Genetics 145:349-58. 1997
    ..The efficiency of this process, its homology dependence, and the clustering of the products define a novel transformation pathway that we call "parahomologous targeting."..
  75. ncbi A model wheat cultivar for transformation to improve resistance to Fusarium Head Blight
    Caroline A Mackintosh
    USDA ARS Plant Science Research Unit and Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, St Paul, MN 55108, USA
    Plant Cell Rep 25:313-9. 2006
    ..Our results indicate that Apogee is particularly well suited for accelerating transgenic FHB resistance research and transgenic wheat research in general...
  76. ncbi High efficiency transgene segregation in co-transformed maize plants using an Agrobacterium tumefaciens 2 T-DNA binary system
    Michael Miller
    Pioneer Hi Bred International Inc, Johnston, Iowa 50131, USA
    Transgenic Res 11:381-96. 2002
    ..A high efficiency system to segregate transgenes in co-transformed maize plants has now been demonstrated...
  77. ncbi Barley transformation using Agrobacterium-mediated techniques
    Wendy A Harwood
    Department of Crop Genetics, John Innes Centre, Colney, Norwich, UK
    Methods Mol Biol 478:137-47. 2009
    ..The method can lead to the production of large numbers of fertile, independent transgenic lines. It is therefore ideal for studies of gene function in a cereal crop system...
  78. ncbi Genetic transformation of switchgrass
    Yajun Xi
    Northwest A and F University, Yangling, Shaanxi, China
    Methods Mol Biol 581:53-9. 2009
    ..Calluses resistant to hygromycin were obtained after 5-6 weeks of selection. Soil-grown switchgrass plants were regenerated about 6 months after callus induction and Agrobacterium-mediated transformation...
  79. ncbi Reef-coral proteins as visual, non-destructive reporters for plant transformation
    A Wenck
    Syngenta Biotechnology Inc, 3054 Cornwallis Road, Research Triangle Park, North Carolina, NC 27709, USA
    Plant Cell Rep 22:244-51. 2003
    ..These proteins will become useful tools for transformation optimization and other studies. The wide variety of plants successfully tested demonstrates that these proteins will potentially find broad use in plant biology...
  80. ncbi Genetic transformation systems in insects
    P W Atkinson
    Department of Entomology, University of California, Riverside, California 92521, USA
    Annu Rev Entomol 46:317-46. 2001
    The past 5 years have witnessed the emergence of techniques that permit the stable genetic transformation of a number of non-drosophilid insect species...
  81. ncbi Expression of a synthetic porcine alpha-lactalbumin gene in the kernels of transgenic maize
    Suk Hwan Yang
    Interdepartmental Genetics Program, Iowa State University, USA
    Transgenic Res 11:11-20. 2002
    ..This suggests that the signal peptide might play an important role in porcine alpha-lactalbumin accumulation in transgenic maize kernels...
  82. ncbi Genetic transformation of conifers utilizing somatic embryogenesis
    Krystyna Klimaszewska
    Natural Resources Canada, Canadian Forest Service, Quebec, Canada
    Methods Mol Biol 286:151-64. 2005
    ..This has allowed us to develop protocols for the genetic transformation of several spruce species...
  83. ncbi Genetic transformation of selected mature cork oak (Quercus suber L.) trees
    R Alvarez
    Department of Biología de Organismos y Sistemas, University of Oviedo, C Catedrático Rodrigo Uría s n, 33011, Spain
    Plant Cell Rep 23:218-23. 2004
    ..Evidence of stable transgene integration was obtained by PCR for the nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos were germinated and successfully transferred to soil...
  84. ncbi Evidence that direct DNA uptake through cut shoots leads to genetic transformation of Solanum aviculare Forst
    P B Gahan
    School of Biomedical Sciences, King s College London, Guy s Campus, London Bridge, London, UK
    Cell Biochem Funct 21:11-7. 2003
    ..There was a relatively high rate of transformation of approximately 5% of the fed stems across all experiments conducted during the present study...
  85. ncbi Genetic transformation of midgut bacteria from the red imported fire ant (Solenopsis invicta)
    Freder Medina
    Department of Entomology, Heep Center, Texas A and M University, College Station, TX 77843 2475, USA
    Curr Microbiol 58:478-82. 2009
    ..Here we report the genetic transformation and reintroduction of three species (Kluyvera cryocrescens, Serratia marcescens, and isolate 38) into the ..
  86. ncbi Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit
    S Ruf
    , , , D-79104 Freiburg, Germany
    Nat Biotechnol 19:870-5. 2001
    ....
  87. ncbi The com locus controls genetic transformation in Streptococcus pneumoniae
    Q Cheng
    Laboratory of Molecular Infectious Diseases, Rockefeller University, New York, New York 10021, USA
    Mol Microbiol 23:683-92. 1997
    ..Finally, we showed genetically and biochemically a CSP-dependent transcription of rec, a competence-induced locus, and that ComD and ComE are required for this CSP-dependent expression...
  88. ncbi Genetic transformation and regeneration of rubber tree (Hevea brasiliensis Muell. Arg) transgenic plants with a constitutive version of an anti-oxidative stress superoxide dismutase gene
    R Jayashree
    Biotechnology Division, Rubber Research Institute of India, Ministry of Commerce and Industry, Kottayam 686 009, Kerala, India
    Plant Cell Rep 22:201-9. 2003
    Agrobacterium tumefaciens-mediated genetic transformation and the regeneration of transgenic plants was achieved in Hevea brasiliensis. Immature anther-derived calli were used to develop transgenic plants...
  89. ncbi Genetic transformation and pyramiding of aprotinin-expressing sugarcane with cry1Ab for shoot borer (Chilo infuscatellus) resistance
    S Arvinth
    Sugarcane Breeding Institute, Coimbatore 641007, India
    Plant Cell Rep 29:383-95. 2010
    ..The potential of producing sugarcane transgenics with cry1Ab and aprotinin genes resistant to early shoot borer was discussed in the light of the results obtained...
  90. pmc The VirE3 protein of Agrobacterium mimics a host cell function required for plant genetic transformation
    Benoît Lacroix
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    EMBO J 24:428-37. 2005
    ....
  91. pmc Early events in Agrobacterium-mediated genetic transformation of citrus explants
    Leandro Peña
    Dpto Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial, 46113 Moncada, Valencia, Spain
    Ann Bot 94:67-74. 2004
    b>Genetic transformation of plants relies on two independent but concurrent processes: integration of foreign DNA into plant cells and regeneration of whole plants from these transformed cells...
  92. pmc Establishing genetic transformation for comparative developmental studies in the crustacean Parhyale hawaiensis
    Anastasios Pavlopoulos
    Institute of Molecular Biology and Biotechnology, 711 10 Iraklio Crete, Greece
    Proc Natl Acad Sci U S A 102:7888-93. 2005
    ..Here, we report the use of the Minos transposable element for the genetic transformation of P. hawaiensis...
  93. ncbi Stable genetic transformation of castor (Ricinus communis L.) via Agrobacterium tumefaciens-mediated gene transfer using embryo axes from mature seeds
    M Sujatha
    Crop Improvement Section, Directorate of Oilseeds Research, Rajendranagar, Hyderabad, 500030, India
    Plant Cell Rep 23:803-10. 2005
    ..This paper reports the first successful attempt at producing transgenic castor...
  94. ncbi Construction of a genomic library of wild rice and Agrobacterium-mediated transformation of large insert DNA linked to BPH resistance locus
    Rui Feng He
    Key Laboratory of Ministry of Education for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China
    Gene 321:113-21. 2003
    ..The system developed here should serve as source for gene discovery, gene cloning and genome-related research in wild rice...
  95. ncbi Evaluation of selection strategies alternative to nptII in genetic transformation of citrus
    Alida Ballester
    Centre of Plant Protection and Biotechnology, Instituto Valenciano de Investigaciones Agrarias IVIA, Apartado Oficial, 46113 Moncada, Valencia, Spain
    Plant Cell Rep 27:1005-15. 2008
    ..Thirdly, the highest transformation rates were obtained with the PMI/mannose system, 30% for citrange and 13% for sweet orange, which indicates that this marker is also an excellent candidate for citrus transformation...
  96. ncbi AtMYBL2, a protein with a single MYB domain, acts as a negative regulator of anthocyanin biosynthesis in Arabidopsis
    Kyoko Matsui
    Research Institute of Genome Based Biofactory, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan
    Plant J 55:954-67. 2008
    ..AtMYBL2 bound directly to TT8 protein and this complex suppressed the expression of DFR and TT8. The repression activity of AtMYBL2 appears to play a critical role in the regulation of anthocyanin biosynthesis...
  97. ncbi Stable genetic transformation of castor (Ricinus communis L.) via particle gun-mediated gene transfer using embryo axes from mature seeds
    M Sailaja
    Crop Improvement Section, Directorate of Oilseeds Research, Rajendranagar, Hyderabad, 500 030, India
    Plant Cell Rep 27:1509-19. 2008
    ..4%. The present results demonstrate the possibility of transformation of embryonic meristematic tissues of castor through particle delivery system...
  98. ncbi A position-effect assay for boundaries of higher order chromosomal domains
    R Kellum
    Department of Biology, Princeton University, New Jersey 08544
    Cell 64:941-50. 1991
    ....
  99. ncbi Genetic transformation and mutant isolation in Ganoderma lucidum by restriction enzyme-mediated integration
    Sunkyung Kim
    Microbial Physiology Laboratory, Division of Life Sciences, Kangwon National University, Chunchon 200 701, South Korea
    FEMS Microbiol Lett 233:201-4. 2004
    ..In order to analyze gene functions and introduce foreign genes into this fungus, genetic transformation is required. We have successfully transformed G...
  100. ncbi Genetic transformation of sweet sorghum
    Anshu Raghuwanshi
    Botany Department BIOL, The University of Queensland, Brisbane, 4072, Australia
    Plant Cell Rep 29:997-1005. 2010
    ..Factors likely to be important for success with other sweet sorghum cultivars are identified...
  101. ncbi Efficient genetic transformation of Sorghum using a visual screening marker
    Zhensheng Gao
    Department of Agronomy, Kansas State University, Manhattan, KS 66506 5501, USA
    Genome 48:321-33. 2005
    ..This report describes the successful use of GFP screening for efficient production of stably transformed sorghum plants without using antibiotics or herbicides as selection agents...

Research Grants62

  1. The effect of inter-species interactions on the virulence of Streptococcus mutans
    Bing yan Wang; Fiscal Year: 2009
    ..biofilm formation, bacteriocin production, acid tolerance, antimicrobial sensitivity, and natural genetic transformation. Elimination of CSP or its signal transduction system results in alterations in all of these virulence ..
  2. Streptococcus pneumoniae genetic competence and pathogenesis
    Michael E Sebert; Fiscal Year: 2011
    ..This bacterium displays natural competence for genetic transformation and devotes considerable regulatory effort to controlling this process...
  3. Biosynthesis and Function of Treponema denticola Lipooligosaccharide
    Hui Wu; Fiscal Year: 2013
    ..However, little is known of T. denticola virulence, as the organism is difficult to grow and the genetic transformation system is not well established. Our goal is to identify virulence factor involved in T...
  4. Developing genetic transformation system in veillonellae
    Fengxia Qi; Fiscal Year: 2012
    ..This project aims to establish a robust Veillonella genetic transformation system...
  5. Small molecules that induce/promote generation of iPS cells
    Sheng Ding; Fiscal Year: 2010
    ..With such proof-of-principle demonstration (which however uses irreversible genetic transformation), an ultimate practical utility of such reprogramming technology would require temporal and reversible ..
  6. Pathogenic Roles of Coxiella burnetti Proteins
    James Samuel; Fiscal Year: 2007
    ..Koch's postulate for the requirement of RecA by creating a transdominant negative mutant through genetic transformation. Finally, we will characterize the role of acid phosphatase in intracellular survival...
  7. VIRULENCE IN COXIELLA BURNETTII
    HERBERT THOMPSON; Fiscal Year: 1999
    ..Individual genes then suspected to be important for virulence will be studied by knockout mutations. The proposed experiments are the first to examine C. burnetii by genetic transformation.
  8. Genetic transformation system for Chlamydia suis
    DANIEL ROCKEY; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): The study of Chlamydia is complicated by the lack of a genetic transformation system. The development of such a system will likely revolutionize research in this area...
  9. PATHOGENIC ROLES OF COXIELLA BURNETTI SURFACE PROTEINS
    James Samuel; Fiscal Year: 2000
    ..molecular Koch's postulate for the requirement of RecA by creating a transdominant negative mutant through genetic transformation. Finally, he will characterize the role that acid phosphatase plays in intracellular survival...
  10. Arrested Development of Arabidopsis
    F Pickett; Fiscal Year: 2003
    ..from each ADD locus and been reintroduced into mutant backgrounds via Agrobacterium mediated plant genetic transformation. In both cases transformation rescue of the mutant phenotype has been observed, indicating that DNA ..
  11. DEVELOPMENTAL BIOLOGY OF THE IMPLANTING MAMMALIAN EMBRYO
    Roger Pedersen; Fiscal Year: 2000
    ..analysis services, and the Cell Culture/Targeted Mutagenesis Core, which provides cell culture and genetic transformation services, including all the activities needed for targeted mutagenesis in mice...
  12. GENE EXPRESSION AND REGULATION IN P. FALCIPARUM
    DAVID BZIK; Fiscal Year: 1992
    ..To facilitate studies of gene expression an in vitro genetic transformation system will be developed...
  13. Examination of PTL-1 in mechanosensory neurons of Caenorhabditis elegans
    KATHLEEN SUSMAN; Fiscal Year: 2007
    ..We will use confocal laser scanning microscopy and immunoprecipitation combined with genetic transformation techniques to reveal the protein-protein interactions of PTL-1 with these microtubules, as well as with ..
  14. MECHANISMS OF FUNCTIONAL SPECIALIZATION OF MICROTUBULES
    Jacek Gaertig; Fiscal Year: 2000
    ..upon a recent achievement by the principal investigator, while he was a postdoctoral fellow, that permits genetic transformation of Tetrahymena by homologous integration...
  15. LARVAL MOSQUITO FEEDING AND MICROBIAL INTERACTIONS
    Edward Walker; Fiscal Year: 2006
    ..vector control methods will be elucidated, including means of manipulating the microbial community with genetic transformation methods...
  16. MOLECULAR ANALYSIS OF STAPHYLOCOCCUS AUREUS AUTOLYSINS
    RADHESHYAM JAYASWAL; Fiscal Year: 1993
    Autolysins have been proposed to play important roles in cell morphogenesis, competence for genetic transformation, and antibiotic induced lysis. They have not, however, been thoroughly investigated at the molecular or genetic level...
  17. Genetic Transformation of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2005
    ..The goal of this project is to develop a transient genetic transformation system for Cryptosporidiumparvum...
  18. IMMUNE RESPONSE OF MOSQUITOES TO FILARIAL WORMS
    Bruce Christensen; Fiscal Year: 2003
    ..e., to change vector competence by genetic transformation to reduce the ability of mosquitoes within a population to transmit a particular pathogen...
  19. RILUZOLE MOA: TARGETS FOR IMPROVED NEUROPROTECTIVE DRUGS
    Ann Sluder; Fiscal Year: 2004
    ..Based on the genetic mapping results, a combination of candidate gene sequencing and, as needed, genetic transformation experiments will be used to identify the specific mutations conferring riluzole resistance...
  20. TRANSFORMATION IN PNEUMOCYSTIS CARINII
    ALAN SMULIAN; Fiscal Year: 2000
    ..The overall goal of this research proposal is to develop a genetic transformation system for Pc...
  21. MOLECULAR GENETICS OF POLYKETIDE BIOSYNTHESIS
    Kathleen Rein; Fiscal Year: 2000
    ..Because no genetic transformation systems have been developed for dinoflagellates, we view this work as somewhat high risk, however the ..
  22. NUCLEASES IN BACILLUS SUBTILIS TRANSFORMATION
    William Burke; Fiscal Year: 1980
    ..to investigate the nature of the deoxyribonucleases involved in the processing of DNA for transport in the genetic transformation of Bacillus subtilis by examining two specific endonucleases found in competent cells, (b) to isolate ..
  23. A Novel Nutritional Intervention to Combat Osteoporosis
    MARK ELLESS; Fiscal Year: 2005
    ..The project also will begin genetic transformation of a popular variety of fresh lettuce to express the CAXgene for testing in Phase II...
  24. EVOLUTIONARY GENETICS OF DNA REPAIR AND RECOMBINATION
    RICHARD MICHOD; Fiscal Year: 1991
    ..The proposed work will test in the eubacterium Bacillus subtilis the hypothesis that natural genetic transformation evolved because of its role in DNA repair...
  25. MARINER MOBILITY IN THE MOSQUITO GENOME
    Craig Coates; Fiscal Year: 2004
    ..The recent development of genetic transformation systems for the mosquito, Aedes aegypti, will allow the expression of these molecules within the mosquito ..
  26. Molecular Mechanisms of Persistent Chlamydial Infections
    Tracy Nicholson; Fiscal Year: 2004
    ..date, research involving Chlamydia and its host interactions has been hampered by the lack of a functional genetic transformation system...
  27. ISOLATION AND STUDY OF MUTANTS OF PLASMODIUM FALCIPARUM
    JOSEPH INSELBURG; Fiscal Year: 1990
    ..The development of a system for the in vitro genetic transformation of P. falciparum will be pursued to facilitate further studies of the parasite.
  28. HOBO-LIKE ELEMENTS IN INSECTS
    DAVID O BROCHTA; Fiscal Year: 2008
    ..gambiae populations, 3) develop Herves into a mosquito gene vector. These aims will be accomplished by performing a combination of laboratory and field-based studies using molecular and population genetics tools. ..
  29. HERMES ELEMENT TRANSPOSITION IN MOSQUITOES
    Peter Atkinson; Fiscal Year: 2008
    ..abstract_text> ..
  30. Tn5 as an insect genomics tool
    DAVID O BROCHTA; Fiscal Year: 2002
    ..the study of human diseases transmitted by insects over the last twenty years has been the development of genetic transformation technology for the human malaria vector, Anopheles gambiae. Genetic transformation systems for An...
  31. Genetic Manipulation of Insects
    DAVID O BROCHTA; Fiscal Year: 2004
    ..The meeting is an uncommon opportunity for graduate students and postdoctoral fellows to obtain a high level synopsis of the field of insect molecular genetics and to make important career-advancing interactions. ..
  32. Adaptive evolution of pathogenesis towards symbiosis
    Serap Aksoy; Fiscal Year: 2009
    ..The role of functions typically associated with pathogenic microorganisms will be pursued in these beneficial microbes. ..
  33. Epigenetic Changes Induced by Estrogens and Xenoestrogens in Breast Cancer
    Jose Russo; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  34. Trypanosome gene expression in tsetse
    Serap Aksoy; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  35. Genomic Markers of Breast Cancer Prevention Induced by hCG in Women at High Risk
    Jose Russo; Fiscal Year: 2008
    ..This knowledge will serve as the basis for establishing novel genomic signatures as intermediate biomarkers for larger preventive clinical trials at the completion of this project. [unreadable] [unreadable] [unreadable]..
  36. Fox Chase Cancer Center Breast Cancer Tissue Resources
    Jose Russo; Fiscal Year: 2008
    ..abstract_text> ..
  37. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2010
    ..Successful completion of these studies would make transgenic chloroplasts ideal bioreactors for the production of safe and less expensive therapeutic proteins and open the door for in depth studies of membrane proteins. ..
  38. INTERACTION OF TSETSE--ITS SYMBIONTS AND TRYPANOSOMES
    Serap Aksoy; Fiscal Year: 2001
    ..The basic knowledge obtained will strengthen our understanding of tsetse biology and will also provide information relevant to other important disease vectors which harbor similar symbionts (e.g., ticks, fleas, reduviid bugs etc.). ..
  39. AIDS Opportunistic Pathogens/Fungal Cell Wall Drug Targets
    Brendan Cormack; Fiscal Year: 2006
    ..Identification of the enzymes responsible for GPI-CWP crosslinking will allow chemotherapeutic targeting of a fungal specific, essential and proven target for development of new antifungal therapy. ..
  40. ROLE OF MICROSATELLITE INSTABILITY IN BREAST CANCER
    Jose Russo; Fiscal Year: 2002
    ..The knowledge gained through this exploratory work will establish the basis for more in dept studies in the role of mismatch repair in breast cancer. ..
  41. Genomic Basis of Reproductive History on Breast Cancer
    Jose Russo; Fiscal Year: 2006
    ..abstract_text> ..
  42. 4-Hydroxyestradiol Receptor in Breast Cancer
    Jose Russo; Fiscal Year: 2005
    ..This plan is in line with goals 1 and 3 of the Breast Cancer Progress Review Group to better understand the biology of the normal mammary gland and of precancerous lesions. ..
  43. pilE mRNA Analysis in Gonococci
    Stuart Hill; Fiscal Year: 2007
    ..These studies should provide unique opportunities for the development of novel pharmaceuticals for therapeutic intervention into gonorrheal or meningococcal disease. [unreadable] [unreadable] [unreadable] [unreadable]..
  44. A Plant-Derived, Multi-Component Tuberculosis Vaccine
    AMANDA WALMSLEY; Fiscal Year: 2004
    ..abstract_text> ..
  45. Repair of Clustered DNA Damages
    Yoke W Kow; Fiscal Year: 2010
    ..In addition, it will provide significant insight as to the potential mechanism involved in melanoma progression. ..
  46. A novel transgenic silkworm system for recombinant glycoprotein production
    Donald L Jarvis; Fiscal Year: 2010
    ..glycosylation pathways for the past decade and the Fraser lab has developed a superb system for efficient genetic transformation of insects, particularly the silkworm...
  47. Novel genetic markers for the yellow fever mosquito
    Zhijian Tu; Fiscal Year: 2003
    ..aegypti in natural populations. ..
  48. Impact of Anti-IL-4 Therapy on Immune Polarization
    Ke Zhang; Fiscal Year: 2003
    ..Overall, the experiments in this proposal will provide direct information as to the impacts of anti-IL-4 therapy on antibody responses and immune responses and by implication, the role of IL-4 in vivo in humans. ..
  49. Repair of Clustered DNA Damages
    YOKE KOW; Fiscal Year: 2008
    ..Completion of these three aims is expected to provide significant understanding on how cells process tandem lesions and the biological consequences of incompletely repaired tandem lesions. ..
  50. ENTOMOLOGICAL ASSUMPTIONS OF DENGUE CONTROL
    Thomas Scott; Fiscal Year: 2002
    ....
  51. Influence of glycosylation on nuclear pore function
    KENNETH BELANGER; Fiscal Year: 2005
    ....
  52. BASIC MECHANISMS IN LUNG DISEASE
    Janet Yother; Fiscal Year: 2007
    ..Based on our program's strong record of productivity and its importance in these endeavors, we seek to continue our role in training young investigators for productive careers in pulmonary research. ..
  53. Anaerobic Regulatory Pathways in a Periodontopathogen
    David Kolodrubetz; Fiscal Year: 2006
    ..Characterizing undefined, potentially new transcriptional regulatory pathways is important because the proteins involved may serve as targets for future drug development. ..
  54. 6th ASM Conference on Streptococcal Genetics
    Janet Yother; Fiscal Year: 2002
    ..The present conference will follow that trend, as we focus on the work of both established and young scientists. ..
  55. Analysis of Plasmodium development in the mosquito
    Marcelo Jacobs Lorena; Fiscal Year: 2006
    ..Knockout mutations will be produced and changes of gene expression caused by these mutations will be measured by use of the micro-arrays. ..
  56. Host Factors in Susceptibility to Chlamydial Disease
    KYLE RAMSEY; Fiscal Year: 2006
    ....
  57. Breaking Tolerance to a Tumor associated Antigen
    Scott Bingham; Fiscal Year: 2005
    ..We believe the novel delivery approach of this vaccine has potential to provide a broad immune response more capable of decreasing if not preventing tumor burden. ..
  58. Pheromone Signaling in Pneumocystis Carinii
    Charles Thomas; Fiscal Year: 2009
    ..Through these investigations we hope to gain insights into P. carinii biology which may provide new information for novel drug development to treat PCP. ..
  59. Adenoviral GFP targeting of metastatic human tumors using multiple delivery route
    Hiroyuki Kishimoto; Fiscal Year: 2008
    ..Having obtained these results, we can move forward in the Phase II grant application with experiments to bring OBP-401 to the clinic for use in cancer surgery in human patients. [unreadable] [unreadable] [unreadable]..