genetic conjugation

Summary

Summary: A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate PROTOZOA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.

Top Publications

  1. ncbi Conjugative transposons: the tip of the iceberg
    Vincent Burrus
    Laboratoire de Génétique et Microbiologie, UMR INRA UHP 1128, IFR110, Faculte des Sciences et Techniques, Universite Henri Poincare Nancy 1, BP239, 54506 Vandoeuvre les Nancy, France
    Mol Microbiol 46:601-10. 2002
  2. pmc Amelioration of the cost of conjugative plasmid carriage in Eschericha coli K12
    Cecilia Dahlberg
    Department of Biology, University of California, San Diego, California 92093, USA
    Genetics 165:1641-9. 2003
  3. ncbi Mobile genetic elements: the agents of open source evolution
    Laura S Frost
    Department of Biological Sciences, Biological Sciences Centre, University of Alberta Edmonton, Alberta T6G 2E9, Canada
    Nat Rev Microbiol 3:722-32. 2005
  4. ncbi SOS response promotes horizontal dissemination of antibiotic resistance genes
    John W Beaber
    Department of Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, 136 Harrison Avenue, Boston, Massachusetts 02111, USA
    Nature 427:72-4. 2004
  5. ncbi Bacterial conjugation: a two-step mechanism for DNA transport
    Matxalen Llosa
    Dipartmento de Biología Molecular, Unidad Asociada al CIB CSIC, Universidad de Cantabria, Santander, Spain
    Mol Microbiol 45:1-8. 2002
  6. ncbi Genetic exchange between bacteria in the environment
    J Davison
    Institut National de la Recherche Agronomique, Route de Saint Cyr, Versailles, F 78026, France
    Plasmid 42:73-91. 1999
  7. pmc Dissemination of sulfonamide resistance genes (sul1, sul2, and sul3) in Portuguese Salmonella enterica strains and relation with integrons
    Patricia Antunes
    Faculdade de Ciências da Nutrição e Alimentação, Universidade do Porto, Rua Anibal Cunha, n o 164, 4050 047 Porto, Portugal
    Antimicrob Agents Chemother 49:836-9. 2005
  8. pmc Conjugative plasmid transfer in gram-positive bacteria
    Elisabeth Grohmann
    Microbial Ecology Group, University of Technology Berlin, D 10587 Berlin, Germany
    Microbiol Mol Biol Rev 67:277-301, table of contents. 2003
  9. pmc Conjugative transfer of the Lactococcus lactis chromosomal sex factor promotes dissemination of the Ll.LtrB group II intron
    Kamila Belhocine
    McGill University, Lyman Duff Medical Building, 3775 University St, Montreal, Quebec, Canada H3A 2B4
    J Bacteriol 187:930-9. 2005
  10. pmc Splicing of a group II intron involved in the conjugative transfer of pRS01 in lactococci
    D A Mills
    Department of Microbiology and Institute for Advanced Studies in Biological Process Technology, University of Minnesota, Minneapolis 55455 0312, USA
    J Bacteriol 178:3531-8. 1996

Research Grants

  1. STRUCTURAL STUDIES OF NUCLEOPROTEIN COMPLEXES
    Edward H Egelman; Fiscal Year: 2012
  2. FASEB CONFERENCE: HELICASES: STRUCTURE, FUNCTION
    Smita Patel; Fiscal Year: 2003
  3. FASEB Summer Conference on Helicase and NTP-Driven Nucleic Acid Motors: Structure
    Timothy Lohman; Fiscal Year: 2007
  4. Delivering drugs via bacterial secretion systems
    Marcin S Filutowicz; Fiscal Year: 2010
  5. GENETIC EXCHANGE BY A BACTERIOPHAGE FROM BARTONELLA
    MICHAEL MINNICK; Fiscal Year: 1999
  6. CELL-PLASMID INTERACTIONS IN BACTERIAL CONJUGATION
    Philip Silverman; Fiscal Year: 1991
  7. GENETIC DETERMINANTS OF VIRULENCE IN HELICOBACTER
    Lucy Tompkins; Fiscal Year: 1993
  8. Amersham Typhoon 9410 Variable Model Imager
    J Michael McCaffery; Fiscal Year: 2005
  9. CONJUGATIVE MOBILIZATION OF PLASMID R1162
    Richard Meyer; Fiscal Year: 2003
  10. Tracking DNA transfer in oral biofilms
    DOMINIQUE GALLI; Fiscal Year: 2007

Detail Information

Publications230 found, 100 shown here

  1. ncbi Conjugative transposons: the tip of the iceberg
    Vincent Burrus
    Laboratoire de Génétique et Microbiologie, UMR INRA UHP 1128, IFR110, Faculte des Sciences et Techniques, Universite Henri Poincare Nancy 1, BP239, 54506 Vandoeuvre les Nancy, France
    Mol Microbiol 46:601-10. 2002
    ..These elements evolve by acquisition or exchanges of modules with various transferable elements including at least ICEs and plasmids. The ICEs are probably widespread among the bacteria...
  2. pmc Amelioration of the cost of conjugative plasmid carriage in Eschericha coli K12
    Cecilia Dahlberg
    Department of Biology, University of California, San Diego, California 92093, USA
    Genetics 165:1641-9. 2003
    ..Our results suggest that once a conjugative bacterial plasmid has invaded a bacterial population it will remain even if the original selection is discontinued...
  3. ncbi Mobile genetic elements: the agents of open source evolution
    Laura S Frost
    Department of Biological Sciences, Biological Sciences Centre, University of Alberta Edmonton, Alberta T6G 2E9, Canada
    Nat Rev Microbiol 3:722-32. 2005
    ..This review describes MGEs, their properties that are important in horizontal gene transfer, and current opportunities to advance MGE genomics...
  4. ncbi SOS response promotes horizontal dissemination of antibiotic resistance genes
    John W Beaber
    Department of Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, 136 Harrison Avenue, Boston, Massachusetts 02111, USA
    Nature 427:72-4. 2004
    ..Thus, we present a mechanism by which therapeutic agents can promote the spread of antibiotic resistance genes...
  5. ncbi Bacterial conjugation: a two-step mechanism for DNA transport
    Matxalen Llosa
    Dipartmento de Biología Molecular, Unidad Asociada al CIB CSIC, Universidad de Cantabria, Santander, Spain
    Mol Microbiol 45:1-8. 2002
    ..It is proposed that this second step is catalysed by the coupling proteins. Our 'shoot and pump' model solves the protein-DNA transport paradox of T4SS...
  6. ncbi Genetic exchange between bacteria in the environment
    J Davison
    Institut National de la Recherche Agronomique, Route de Saint Cyr, Versailles, F 78026, France
    Plasmid 42:73-91. 1999
    ....
  7. pmc Dissemination of sulfonamide resistance genes (sul1, sul2, and sul3) in Portuguese Salmonella enterica strains and relation with integrons
    Patricia Antunes
    Faculdade de Ciências da Nutrição e Alimentação, Universidade do Porto, Rua Anibal Cunha, n o 164, 4050 047 Porto, Portugal
    Antimicrob Agents Chemother 49:836-9. 2005
    ..The sul3 gene has been identified in isolates from different sources and serotypes, which also carried a class 1 integron with aadA and dfrA gene cassettes...
  8. pmc Conjugative plasmid transfer in gram-positive bacteria
    Elisabeth Grohmann
    Microbial Ecology Group, University of Technology Berlin, D 10587 Berlin, Germany
    Microbiol Mol Biol Rev 67:277-301, table of contents. 2003
    ....
  9. pmc Conjugative transfer of the Lactococcus lactis chromosomal sex factor promotes dissemination of the Ll.LtrB group II intron
    Kamila Belhocine
    McGill University, Lyman Duff Medical Building, 3775 University St, Montreal, Quebec, Canada H3A 2B4
    J Bacteriol 187:930-9. 2005
    ..LtrB intron. Our findings explain the unusually high efficiency of Ll.LtrB mobility observed following conjugation of intron-containing plasmids...
  10. pmc Splicing of a group II intron involved in the conjugative transfer of pRS01 in lactococci
    D A Mills
    Department of Microbiology and Institute for Advanced Studies in Biological Process Technology, University of Minnesota, Minneapolis 55455 0312, USA
    J Bacteriol 178:3531-8. 1996
    ..These results provide the first direct evidence for splicing of a prokaryotic group II intron in vivo and suggest that conjugative transfer is a mechanism for group II intron dissemination in bacteria...
  11. pmc An intrastrand three-DNA-base interaction is a key specificity determinant of F transfer initiation and of F TraI relaxase DNA recognition and cleavage
    Katherine Hekman
    Department of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Nucleic Acids Res 36:4565-72. 2008
    ..Oligonucleotide cleavage assay results suggest the essential function of the three-base interaction may be to position the scissile phosphate for cleavage, rather than to directly contribute to binding affinity...
  12. ncbi Why is entry exclusion an essential feature of conjugative plasmids?
    M Pilar Garcillán-Barcia
    Departamento de Biología Molecular e Instituto de Biomedicina y Biotecnología de Cantabria IBBTEC, Universidad de Cantabria CSIC IDICAN, C Herrera Oria s n, 39011 Santander, Spain
    Plasmid 60:1-18. 2008
    ..Additionally, it avoids competition in a host among identical plasmid backbones. Conversely, the lack of entry exclusion in conjugative transposons can be understood as a means of generating rapid evolutionary change...
  13. pmc Analysis of DNA processing reactions in bacterial conjugation by using suicide oligonucleotides
    Blanca Gonzalez-Perez
    Departamento de Biologia Molecular, Universidad de Cantabria, and Instituto de Biomedicina y Biotecnología de Cantabria, CSIC UC IDICAN, Santander, Spain
    EMBO J 26:3847-57. 2007
    ..Thus, only Y18 was used for initiation. Y26 was specifically used in the second transesterification that leads to strand transfer, thus catalyzing the termination reaction that occurs in the recipient cell...
  14. pmc A bacteroides conjugative transposon, CTnERL, can transfer a portion of itself by conjugation without excising from the chromosome
    Gabrielle Whittle
    Department of Microbiology, 601 S Goodwin Ave, University of Illinois, Urbana, IL 61801, USA
    J Bacteriol 188:1169-74. 2006
    ....
  15. ncbi Genetic evidence of a coupling role for the TraG protein family in bacterial conjugation
    E Cabezon
    Departamento de Biologia Molecular, Universidad de Cantabria, Santander, Spain
    Mol Gen Genet 254:400-6. 1997
    ..Therefore we suggest that the factors that determine the frequencies of transfer of different MOB regions are the differential interactions of TrwB with pilus and relaxosome...
  16. ncbi F factor conjugation is a true type IV secretion system
    T D Lawley
    Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada T6G 2E9
    FEMS Microbiol Lett 224:1-15. 2003
    ..This review examines the similarities and differences among the T4SS, especially F- and P-like systems, and summarizes the properties of the F transfer region gene products...
  17. ncbi Conjugative DNA metabolism in Gram-negative bacteria
    Fernando de la Cruz
    Department of Molecular Biology, Universidad de Cantabria, Santander, Spain
    FEMS Microbiol Rev 34:18-40. 2010
    ..Here, we review the biochemistry of relaxosomes and ponder some of the remaining questions about the nature of the signal that begins the process...
  18. pmc Extended-spectrum beta-lactamases among Enterobacter isolates obtained in Tel Aviv, Israel
    Jacob Schlesinger
    Division of Epidemiology, Tel Aviv Sourasky Medical Center, 6 Weizmann St, Tel Aviv 64239, Israel
    Antimicrob Agents Chemother 49:1150-6. 2005
    ..The dominant ESBL identified was SHV-12...
  19. pmc The persistence of parasitic plasmids
    Loukia N Lili
    Department of Mathematical Sciences, University of Bath, BA2 7AY, Bath, United Kingdom
    Genetics 177:399-405. 2007
    ....
  20. pmc A new sulfonamide resistance gene (sul3) in Escherichia coli is widespread in the pig population of Switzerland
    Vincent Perreten
    Institute of Veterinary Bacteriology, University of Bern, Switzerland
    Antimicrob Agents Chemother 47:1169-72. 2003
    ..coli. Two copies of the insertion element IS15Delta/26 flanked the region containing sul3. The sul3 gene was detected in one-third of the sulfonamide-resistant pathogenic E. coli isolates from pigs in Switzerland...
  21. pmc Characterization of genes involved in modulation of conjugal transfer of the Bacteroides conjugative transposon CTnDOT
    Gabrielle Whittle
    Department of Microbiology, University of Illinois, Urbana 61801, USA
    J Bacteriol 184:3839-47. 2002
    ..Conversely, in the absence of tetracycline, a gene located near the 3' end of exc is responsible for the repression of transfer protein expression and also results in a 100- to 1,000-fold decrease in the frequency of pLYL72 transfer...
  22. pmc Worldwide disseminated armA aminoglycoside resistance methylase gene is borne by composite transposon Tn1548
    M Galimand
    Unite des Agents Antibacteriens, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 49:2949-53. 2005
    ..It thus appears that posttranscriptional modification of 16S rRNA confers high-level resistance to all the clinically available aminoglycosides except streptomycin in gram-negative human and animal pathogens...
  23. ncbi Splicing of a group II intron in a functional transfer gene of Lactococcus lactis
    C Shearman
    Institute of Food Research, Colney, Norwich, UK
    Mol Microbiol 21:45-53. 1996
    ..Here we report the characterization of a new group II intron in the Gram-positive bacterium L. lactis and demonstrate for the first time in bacteria both splicing in vivo and an active role for the gene carrying the intron...
  24. ncbi Environmental Vibrio spp., isolated in Mozambique, contain a polymorphic group of integrative conjugative elements and class 1 integrons
    Elisa Taviani
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, MD, USA
    FEMS Microbiol Ecol 64:45-54. 2008
    ....
  25. ncbi Modular architecture of the conjugative plasmid pSVH1 from Streptomyces venezuelae
    Jens Reuther
    Microbiology Biotechnology, Microbiological Institute, Faculty of Biology, University of Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    Plasmid 55:201-9. 2006
    ....
  26. ncbi Conjugative transfer of clostridial shuttle vectors from Escherichia coli to Clostridium difficile through circumvention of the restriction barrier
    Des Purdy
    Research Division, Centre for Applied Microbiology and Research, Wiltshire, UK
    Mol Microbiol 46:439-52. 2002
    ..The transfer efficiencies achieved with both strains equated to between 1.0 x 10-6 and 5.5 x 10-5 transconjugants per donor...
  27. pmc Characterization of the 13-kilobase ermF region of the Bacteroides conjugative transposon CTnDOT
    G Whittle
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    Appl Environ Microbiol 67:3488-95. 2001
    ..Therefore, it appears that CTnDOT-like elements present in community and clinical isolates of Bacteroides were derived from a common ancestor and proliferated in the diverse Bacteroides population...
  28. ncbi The integrative element pSAM2 from Streptomyces: kinetics and mode of conjugal transfer
    C Possoz
    Laboratoire de Biologie et Génétique Moléculaire, Institut de Genetique et Microbiologie, UMR CNRS 8621, Bat 400, Universite Paris Sud, F 91405 Orsay cedex, France
    Mol Microbiol 42:159-66. 2001
    ....
  29. ncbi The effect of the Salmonella genomic island 1 on in vitro global gene expression in Salmonella enterica serovar Typhimurium LT2
    George R Golding
    National Microbiology Laboratory, 1015 Arlington Ave, Winnipeg, Manitoba, Canada, R3E 3R2
    Microbes Infect 9:21-7. 2007
    ..This is the first report examining gene expression within the SGI1, as well as its potential effect on global gene expression, and sets the foundation for future studies involving the effect of SGI1 in other Salmonella spp...
  30. ncbi Two atypical mobilization proteins are involved in plasmid CloDF13 relaxation
    B Nunez
    Departamento de Biología Molecular Unidad Asociada al CIB, CSIC, Universidad de Cantabria, C Herrera Oria s n, 39011 Santander, Spain
    Mol Microbiol 39:1088-99. 2001
    ..This is the first report of a conjugative transfer system in which nic cleavage results in a free nicked-DNA intermediate...
  31. ncbi Critical variations of conjugational DNA transfer into secondary metabolite multiproducing Sorangium cellulosum strains So ce12 and So ce56: development of a mariner-based transposon mutagenesis system
    Maren Kopp
    Gesellschaft für Biotechnologische Forschung mbH GBF, Mascheroder Weg 1, 38124 Braunschweig, Germany
    J Biotechnol 107:29-40. 2004
    ..The transposon randomly integrates into the chromosome of both strains. As a proof of principle, S. cellulosum So ce12 transposon mutants were screened using an overlay assay to target the chivosazole biosynthetic gene cluster...
  32. ncbi Conjugation mediates transfer of the Ll.LtrB group II intron between different bacterial species
    Kamila Belhocine
    Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada H3A 2B4
    Mol Microbiol 51:1459-69. 2004
    ....
  33. pmc Composition, acquisition, and distribution of the Vi exopolysaccharide-encoding Salmonella enterica pathogenicity island SPI-7
    Derek Pickard
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College of Science, Technology and Medicine, Armstrong Road, London SW7 2AZ, UK
    J Bacteriol 185:5055-65. 2003
    ..citri. This analysis suggested that SPI-7 may be a mobile element, such as a conjugative transposon or an integrated plasmid remnant...
  34. ncbi Dissemination of 16S rRNA methylase-mediated highly amikacin-resistant isolates of Klebsiella pneumoniae and Acinetobacter baumannii in Korea
    Hyukmin Lee
    Department of Laboratory Medicine, Kwandong University College of Medicine, Kyunggido 412 270, South Korea
    Diagn Microbiol Infect Dis 56:305-12. 2006
    ..Most K. pneumoniae isolates also produced extended-spectrum beta-lactamases and plasmid-mediated AmpC beta-lactamases, and most A. baumannii isolates were nonsusceptible to carbapenems...
  35. ncbi The ClosTron: a universal gene knock-out system for the genus Clostridium
    John T Heap
    Institute of Infection, Immunity and Inflammation, School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    J Microbiol Methods 70:452-64. 2007
    ..The procedure is highly efficient and reproducible, and should revolutionize functional genomic studies in clostridia...
  36. pmc The ins and outs of DNA transfer in bacteria
    Ines Chen
    Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
    Science 310:1456-60. 2005
    ..All or nearly all the machine components involved in transformation and conjugation have been identified, and here we present models for their roles in DNA transport...
  37. ncbi The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants
    C Rayssiguier
    Institut Jacques Monod, Paris, France
    Nature 342:396-401. 1989
    ..This finding has implications for speciation, for regulating recombination between diverged repeated sequences, and for hitherto difficult interspecies hybridizations...
  38. pmc Involvement of a plasmid in virulence of Campylobacter jejuni 81-176
    D J Bacon
    Enteric Diseases Department, Naval Medical Research Center, Silver Spring, Maryland 20910, USA
    Infect Immun 68:4384-90. 2000
    ..The virB11 homolog was detected on plasmids in 6 out of 58 fresh clinical isolates of C. jejuni, suggesting that plasmids are involved in the virulence of a subset of C. jejuni pathogens...
  39. ncbi Plasmid promiscuity: meeting the challenge of DNA immigration control
    Brian M Wilkins
    Department of Genetics, University of Leicester, Leicester LE1 7RH, UK
    Environ Microbiol 4:495-500. 2002
  40. pmc Characterization of integrative and conjugative element ICEKp1-associated genomic heterogeneity in a Klebsiella pneumoniae strain isolated from a primary liver abscess
    Tzu Lung Lin
    Department of Microbiology, National Taiwan University College of Medicine, 1, Sec 1, Jen Ai Rd, Taipei, Taiwan
    J Bacteriol 190:515-26. 2008
    ..Therefore, ICEKp1 may contribute to the transmission of the HPI and result in K. pneumoniae PLA infection-associated genomic heterogeneity...
  41. pmc Emergence of plasmid-mediated quinolone resistance in Escherichia coli in Europe
    Hedi Mammeri
    Service de Bacteriologie Virologie, Hopital de Bicetre, 78 rue du General Leclerc, 94275 Le Kremlin Bicetre cedex, France
    Antimicrob Agents Chemother 49:71-6. 2005
    ..This is the first report of plasmid-mediated quinolone resistance in Europe associated with an unknown level of plasmid-mediated multidrug resistance in Enterobacteriaceae...
  42. pmc Multiple CTX-M-type extended-spectrum beta-lactamases in nosocomial isolates of Enterobacteriaceae from a hospital in northern Italy
    Laura Pagani
    Dipartimento di Scienze Morfologiche Eidologiche e Cliniche, Sezione di Microbiologia, Universita di Pavia, Italy
    J Clin Microbiol 41:4264-9. 2003
    ..This is also the first report of CTX-M-2 in P. vulgaris...
  43. pmc Regulation of a Bacillus subtilis mobile genetic element by intercellular signaling and the global DNA damage response
    Jennifer M Auchtung
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 102:12554-9. 2005
    ..The dual regulation of ICEBs1 allows for passive propagation in the host cell until either the potential mating partners lacking ICEBs1 are present or the host cell is in distress...
  44. ncbi Transfer of plasmid pTO1 from Escherichia coli to various representatives of the order Actinomycetales by intergeneric conjugation
    T Voeykova
    State Scientific Centre of the Russian Federation, GNIIGENETIKA, Moscow, Russia
    FEMS Microbiol Lett 162:47-52. 1998
    ..The frequency of conjugant formation was 1 x 10(-3)-1 x 10(-5) depending on the strain. Hybridization experiments demonstrated that plasmid pTO1 integrates into chromosomes of a number of the recipient strains examined...
  45. pmc Nucleotide sequence analysis of the enteropathogenic Escherichia coli adherence factor plasmid pMAR7
    Carl Brinkley
    Department of Microbiology and Immunology, Center for Vaccine Development, University of Maryland School of Medicine, 685 West Baltimore St, Baltimore, MD 21201, USA
    Infect Immun 74:5408-13. 2006
    ..This study confirms EPEC's potential for dissemination of virulence attributes by horizontal transfer of the EAF plasmid...
  46. ncbi Cut and move: protein machinery for DNA processing in bacterial conjugation
    F Xavier Gomis-Ruth
    Institut de Biologia Molecular de Barcelona CSIC, Parc Cientific de Barcelona, Josep Samitier 1 5, 08028 Barcelona, Spain
    Curr Opin Struct Biol 16:744-52. 2006
    ..In particular, structural biology is revealing the detailed molecular architecture of several of the pieces involved...
  47. ncbi The role of conjugative transposons in the Enterobacteriaceae
    J T Pembroke
    Molecular Biochemistry Group, University of Limerick, Limerick, Ireland
    Cell Mol Life Sci 59:2055-64. 2002
    ..Recent evidence suggests that in the Enterobacteriaceae the elements may be related to enteric pathogenicity islands. The evolution, distribution and role of these elements in the Enterobacteriaceae is discussed...
  48. pmc Plasmids spread very fast in heterogeneous bacterial communities
    Francisco Dionisio
    Faculté de Médicine Necker Enfants Malade, INSERM U571, Universite Paris V, 75730 Paris Cedex 15, France
    Genetics 162:1525-32. 2002
    ..This "amplification effect" could have an impact on the evolution of bacterial pathogens that exist in heterogeneous bacterial communities because conjugative plasmids can carry virulence or antibiotic-resistance genes...
  49. pmc Genomic and functional analyses of SXT, an integrating antibiotic resistance gene transfer element derived from Vibrio cholerae
    John W Beaber
    Division of Geographic Medicine Infectious Diseases, New England Medical Center and Tufts University, and Howard Hughes Medical Institute, Boston, Massachusetts 02111, USA
    J Bacteriol 184:4259-69. 2002
    ..Another locus, designated setR, whose gene product bears similarity to lambdoid phage CI repressors, also appears to regulate SXT gene expression...
  50. pmc Dissemination of CTX-M-15 beta-lactamase genes carried on Inc FI and FII plasmids among clinical isolates of Escherichia coli in a university hospital in Istanbul, Turkey
    Nevriye Gonullu
    Department of Microbiology and Clinical Microbiology, Cerrahpasa Faculty of Medicine, University of Istanbul, Istanbul 34303, Turkey
    J Clin Microbiol 46:1110-2. 2008
    ..Plasmid typing of transconjugates carrying bla(CTX-M-15) showed that most isolates belonged to the Inc/rep FII group but that one isolate also belonged to the FI group...
  51. ncbi The mating pair formation system of conjugative plasmids-A versatile secretion machinery for transfer of proteins and DNA
    Gunnar Schröder
    Division of Molecular Microbiology, Biozentrum, University of Basel, Klingelbergstrasse 50 70, CH 4056 Basel, Switzerland
    Plasmid 54:1-25. 2005
    ..We here present a unified and updated view of the functioning and the molecular architecture of the Mpf/CP machinery...
  52. pmc A conjugation-based system for genetic analysis of group II intron splicing in Lactococcus lactis
    Joanna R Klein
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    J Bacteriol 186:1991-8. 2004
    ..Once the system was validated for the engineered mutants, random mutagenesis of the intron followed by genetic and molecular screening for splicing defects resulted in identification of point mutations that affect splicing...
  53. pmc Formation of SXT tandem arrays and SXT-R391 hybrids
    Vincent Burrus
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA
    J Bacteriol 186:2636-45. 2004
    ..Thus, arrays of SXT-related elements promote the creation of novel ICEs...
  54. ncbi The current ICE age: biology and evolution of SXT-related integrating conjugative elements
    Vincent Burrus
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, and Howard Hughes Medical Institute Boston, MA 02111, USA
    Plasmid 55:173-83. 2006
    ..In this review, we discuss the diversity, evolution and biology of this family of ICEs...
  55. pmc Comparison of proteins involved in pilus synthesis and mating pair stabilization from the related plasmids F and R100-1: insights into the mechanism of conjugation
    K G Anthony
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9
    J Bacteriol 181:5149-59. 1999
    ..Our results indicate that the specificity in recipient cell recognition and entry exclusion are mediated by TraN and TraG, respectively, and not by the pilus...
  56. pmc Examination of an inverted repeat within the F factor origin of transfer: context dependence of F TraI relaxase DNA specificity
    Sarah L Williams
    Department of Biology, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Nucleic Acids Res 34:426-35. 2006
    ....
  57. ncbi Two active-site tyrosyl residues of protein TrwC act sequentially at the origin of transfer during plasmid R388 conjugation
    G Grandoso
    Departamento de Biologia Molecular, Universidad de Cantabria, c Cardenal Herrera Oria s n, Santander, 39011, Spain
    J Mol Biol 295:1163-72. 2000
    ..The model suggests a control mechanism that can be effective at each conjugative replication cycle...
  58. pmc Site-specific recombinase and integrase activities of a conjugative relaxase in recipient cells
    Olga Draper
    Departamento de Biología Molecular Unidad asociada al Centro de Investigaciones Biológicas Consejo Superior de Investigaciones Científicas, Universidad de Cantabria, c Herrera Oria, s n 39011 Santander, Spain
    Proc Natl Acad Sci U S A 102:16385-90. 2005
    ..This TrwC site-specific integration activity in recipient cells may lead to future biotechnological applications...
  59. pmc Enterobacter cloacae outbreak and emergence of quinolone resistance gene in Dutch hospital
    Armand Paauw
    Eijkman Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
    Emerg Infect Dis 12:807-12. 2006
    ..On the same plasmid, 2 other class 1 integrons were present. One was a new integron associated with the bla(CTX-M-9) extended-spectrum beta-lactamase...
  60. pmc Identification of the rctA gene, which is required for repression of conjugative transfer of rhizobial symbiotic megaplasmids
    Daniel Pérez-Mendoza
    Dpto Microbiología del Suelo y Sistemas Simbióticos, Estacion Experimental del Zaidin, Profesor Albareda 1, 18008 Granada, Spain
    J Bacteriol 187:7341-50. 2005
    ..The rctA gene seems to represent novel but probably widespread regulatory systems controlling the transfer of conjugative elements within the order Rhizobiales...
  61. pmc The r1162 mob proteins can promote conjugative transfer from cryptic origins in the bacterial chromosome
    Richard Meyer
    Institute for Cellular and Molecular Biology, University of Texas at Austin, 78712, USA
    J Bacteriol 191:1574-80. 2009
    ..Transfer of chromosomal DNA due to the presence of a plasmid in the cytoplasm describes a previously unrecognized potential for the exchange of bacterial DNA...
  62. ncbi A novel Salmonella genomic island 1 and rare integron types in Salmonella Typhimurium isolates from horses in The Netherlands
    An T T Vo
    Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    J Antimicrob Chemother 59:594-9. 2007
    ..To investigate the genotypic resistance of integron-carrying Salmonella Typhimurium isolates from horses and their genetic relationship...
  63. pmc Genetic manipulation of Prochlorococcus strain MIT9313: green fluorescent protein expression from an RSF1010 plasmid and Tn5 transposition
    Andrew C Tolonen
    Department of Biology, Massachusetts Institute of Technology Woods Hole Oceanographic Institution Joint Program in Biological Oceanography, Cambridge 02139, USA
    Appl Environ Microbiol 72:7607-13. 2006
    ..These genetic advances provide a basis for future genetic studies with Prochlorococcus, a microbe of ecological importance in the world's oceans...
  64. pmc Plasmids imparting sulfonamide resistance in Escherichia coli: implications for persistence
    David C Bean
    Centre for Infectious Disease, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom
    Antimicrob Agents Chemother 53:1088-93. 2009
    ....
  65. ncbi Dynamics of the IncW genetic backbone imply general trends in conjugative plasmid evolution
    Raul Fernandez-Lopez
    Departamento de Biología Molecular Unidad Asociada al C I B, C S I C, Universidad de Cantabria, Santander, Spain
    FEMS Microbiol Rev 30:942-66. 2006
    ..Starting from the phage paradigm, our analysis articulates novel concepts that apply to plasmid evolution...
  66. ncbi Conjugative transfer in the dissemination of beta-lactam and aminoglycoside resistance
    V L Waters
    Department of Medicine, University of California, San Diego Medical School, 9500 Gilman Drive, La Jolla, California 92093, USA
    Front Biosci 4:D433-56. 1999
    ....
  67. pmc Characterization of the mobilization region of a Bacteroides insertion element (NBU1) that is excised and transferred by Bacteroides conjugative transposons
    L Y Li
    Department of Microbiology, University of Illinois, Urbana 61801
    J Bacteriol 175:6588-98. 1993
    ..There was, however, a high level of similarity between the deduced amino acid sequence of the mob product and that of the product of a Bacteroides vulgatus cryptic open reading frame closely linked to a cefoxitin resistance gene (cfxA)...
  68. pmc Plasmid-mediated quinolone resistance in clinical isolates of Escherichia coli from Shanghai, China
    Minggui Wang
    Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, China
    Antimicrob Agents Chemother 47:2242-8. 2003
    ..coli from Shanghai and may contribute to the rapid increase in bacterial resistance to quinolones in China...
  69. ncbi Horizontal gene transfer from transgenic plants to terrestrial bacteria--a rare event?
    K M Nielsen
    UNIGEN Center for Molecular Biology, Norwegian University of Science and Technology, Trondheim, Norway
    FEMS Microbiol Rev 22:79-103. 1998
    ..Only an accurate understanding of these selective events will allow the prediction of possible consequences of novel genes following their introduction into open environments...
  70. ncbi Conjugation between bacterial and mammalian cells
    V L Waters
    Department of Medicine, School of Medicine University of California, San Diego, La Jolla, California 92093 0640, USA
    Nat Genet 29:375-6. 2001
    ..Here, I present evidence of such transfer, using Escherichia coli, the RK2 plasmid system and Chinese hamster ovary CHO K1 cells...
  71. ncbi Conjugal immunity of Streptomyces strains carrying the integrative element pSAM2 is due to the pif gene (pSAM2 immunity factor)
    Christophe Possoz
    Institut de Genetique et Microbiologie, UMR CNRS 8621, Universite Paris Sud, Orsay, France
    Mol Microbiol 47:1385-93. 2003
    ..The Pif nudix motif is essential for its activity. This is the first protein of the nudix family shown to be involved in bacterial conjugation...
  72. pmc Nosocomial outbreak caused by Salmonella enterica serotype Livingstone producing CTX-M-27 extended-spectrum beta-lactamase in a neonatal unit in Sousse, Tunisia
    Olfa Bouallègue-Godet
    Centre national de référence des Salmonella, Unité de Biodiversité des Bactéries Pathogènes Emergentes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Clin Microbiol 43:1037-44. 2005
    ..The babies were treated with colistin, and all except two recovered. The outbreak came to an end when appropriate actions were taken: patient isolation, hand washing, and disinfection of the ward...
  73. ncbi The Salmonella genomic island 1 is an integrative mobilizable element
    Benoit Doublet
    Unité BioAgresseurs, santé, Environnement, Institut National de la Recherche Agronomique, 37380 Nouzilly, France
    Mol Microbiol 55:1911-24. 2005
    ..enterica and E. coli chromosome. SGI1 appeared to be transmissible only in the presence of additional conjugative functions provided in trans. SGI1 can thus be classified within the group of integrative mobilizable elements (IMEs)...
  74. ncbi F plasmid conjugative DNA transfer: the TraI helicase activity is essential for DNA strand transfer
    S W Matson
    Department of Biology and the Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill 27599, USA
    J Biol Chem 276:2372-9. 2001
    ..Thus, both the transesterase and helicase activities of TraI are essential for DNA strand transfer...
  75. pmc Type IV secretion: intercellular transfer of macromolecules by systems ancestrally related to conjugation machines
    P J Christie
    Department of Microbiology and Molecular Genetics, The University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Mol Microbiol 40:294-305. 2001
    ..The list of putative type IV family members is increasing rapidly, suggesting that macromolecular transfer by these systems is a widespread phenomenon in nature...
  76. pmc Association of plasmid-mediated quinolone resistance with extended-spectrum beta-lactamase VEB-1
    Laurent Poirel
    Service de Bacteriologie Virologie, Hopital de Bicetre, Assistance Publique Hopitaux de Paris, Faculte de Medecine Paris Sud, Universite Paris XI, Le Kremlin Bicetre, France
    Antimicrob Agents Chemother 49:3091-4. 2005
    ..Bicêtre, France, and in 11 out of 23 bla(VEB-1)-positive enterobacterial isolates from Bangkok, Thailand. This result may explain in part the association between quinolone and extended-spectrum beta-lactam resistance...
  77. ncbi The role of Bacteroides conjugative transposons in the dissemination of antibiotic resistance genes
    G Whittle
    Department of Microbiology, 601 S Goodwin Ave, University of Illinois, Urbana, Illinois 61801, USA
    Cell Mol Life Sci 59:2044-54. 2002
    ..Since these constitutively transferring elements do not require induction by antibiotics to stimulate transfer, they have the potential to become as pervasive as the CTnDOT/ERL family of conjugative transposons...
  78. ncbi Conjugative transfer can be inhibited by blocking relaxase activity within recipient cells with intrabodies
    M Pilar Garcillán-Barcia
    Departamento de Biología Molecular Laboratorio asociado al Centro de Investigaciones Biológicas, C S I C, Universidad de Cantabria, c Cardenal Herrera Oria s n, 39011 Santander, Spain
    Mol Microbiol 63:404-16. 2007
    ..These findings demonstrate that the transferred relaxase plays an important role in the recipient cell and open a new approach to identify specific inhibitors of bacterial conjugation...
  79. pmc Horizontal transfer of CS1 pilin genes of enterotoxigenic Escherichia coli
    Barbara Froehlich
    Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Bacteriol 186:3230-7. 2004
    ..We conclude that when R64-like plasmids are transmitted to an ETEC strain containing pCoo, their recombination may allow for spread of the pCoo plasmid to other enteric bacteria...
  80. ncbi A variant type of Vibrio cholerae SXT element in a multidrug-resistant strain of Vibrio fluvialis
    Ashraf M Ahmed
    Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, 1 4 4 Kagamiyama, Higashi Hiroshima 739 8528, Japan
    FEMS Microbiol Lett 242:241-7. 2005
    ..Here, we report the detection of a variant SXT element in species other than V. cholerae, with molecular characterization and analysis of its integrase gene and its attP site...
  81. ncbi Proteus mirabilis clinical isolate harbouring a new variant of Salmonella genomic island 1 containing the multiple antibiotic resistance region
    Ashraf M Ahmed
    Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima 739 8528, Japan
    J Antimicrob Chemother 59:184-90. 2007
    ....
  82. ncbi Prevalence of qnr genes in Salmonella in France
    Vincent Cattoir
    Service de Bacteriologie Virologie, Hopital de Bicetre, Assistance Publique Hopitaux de Paris, Faculte de Medecine Paris Sud, Universite Paris XI, K Bicêtre, France
    J Antimicrob Chemother 59:751-4. 2007
    ..To detect the qnrA, qnrB and qnrS genes among Salmonella isolates received at the French National Reference Centre for Salmonella in Paris, France...
  83. ncbi Small RNAs in genome rearrangement in Tetrahymena
    Kazufumi Mochizuki
    Department of Biology, University of Rochester, Hutchison Hall 425, Rochester, New York 14627, USA
    Curr Opin Genet Dev 14:181-7. 2004
    ..These findings contribute to a new paradigm of chromatin biology: regulation of heterochromatin formation by RNAi-related mechanisms in eukaryotes...
  84. pmc Identification of operators and promoters that control SXT conjugative transfer
    John W Beaber
    Department of Microbiology, Tufts University School of Medicine, 136 Harrison Ave, Jaharis 425, Boston, MA 02111, USA
    J Bacteriol 186:5945-9. 2004
    ..SetR binds to four operators located between setR and s086; the locations and relative affinities of these sites suggest a model for regulation of SXT transfer...
  85. pmc Regulation of excision genes of the Bacteroides conjugative transposon CTnDOT
    Kyung Moon
    Department of Microbiology, 601 S Goodwin Ave, University of Illinois, Urbana, IL 61801, USA
    J Bacteriol 187:5732-41. 2005
    ..Taken together, our results support the hypothesis that RteC acts as a DNA-binding protein that binds upstream of the orf2c promoter and is responsible for tetracycline-regulated transcriptional regulation of the orf2c operon...
  86. ncbi Unique conjugation mechanism in mycelial streptomycetes: a DNA-binding ATPase translocates unprocessed plasmid DNA at the hyphal tip
    Jens Reuther
    Mikrobiologie Biotechnologie, Mikrobiologisches Institut, Fakultaet für Biologie, Eberhard Karls Universitaet Tuebingen, Auf der Morgenstelle 28, 72076 Tuebingen, Germany
    Mol Microbiol 61:436-46. 2006
    ....
  87. ncbi Plasmid-mediated 16S rRNA methylases conferring high-level aminoglycoside resistance in Escherichia coli and Klebsiella pneumoniae isolates from two Taiwanese hospitals
    Jing Jou Yan
    Department of Pathology, National Cheng Kung University Hospital, No 138, Sheng Li Rd, Tainan 70428
    J Antimicrob Chemother 54:1007-12. 2004
    ....
  88. ncbi Unsaturated fatty acids are inhibitors of bacterial conjugation
    Raul Fernandez-Lopez
    Departamento de Biología Molecular Unidad Asociada al CIB, CSIC, Universidad de Cantabria, C Herrera Oria s n, E 39011 Santander, Spain
    Microbiology 151:3517-26. 2005
    ..Two separate compounds were identified from these fractions: linoleic acid and dehydrocrepenynic acid. Downstream analysis showed that the chemical class of unsaturated fatty acids act as true inhibitors of conjugation...
  89. ncbi Regulation of conjugal transfer by Lrp and Dam methylation in plasmid R100
    Eva M Camacho
    Department of Genetics, Faculty of Biology, University of Seville, Spain
    Int Microbiol 8:279-85. 2005
    ..Repression of conjugal transfer in rich medium is an additional trait shared by R100 and pSLT. DNA sequence comparisons in regulatory loci support the view that R100 and pSLT are closely related...
  90. ncbi The chloramphenicol resistance gene cmlA is disseminated on transferable plasmids that confer multiple-drug resistance in swine Escherichia coli
    Kenneth M Bischoff
    Southern Plains Agricultural Research Center, US Department of Agriculture, Agricultural Research Service, College Station, TX 77845, USA
    FEMS Microbiol Lett 243:285-91. 2005
    ....
  91. ncbi Holding back the tide of antibiotic resistance
    Wendy Wolfson
    Chem Biol 13:1-3. 2006
  92. ncbi Community and hospital spread of Escherichia coli producing CTX-M extended-spectrum beta-lactamases in the UK
    N Woodford
    Antibiotic Resistance Monitoring and Reference Laboratory, Specialist and Reference Microbiology Division Colindale, Health Protection Agency, London
    J Antimicrob Chemother 54:735-43. 2004
    ..e. MICs of cefotaxime > or = 8-fold higher than MICs of ceftazidime. Many were referred as being from community patients. We examined 291 CTX-M-producing isolates from the UK and investigated the genetic basis of their phenotype...
  93. pmc Construction and characterization of a Bacteroides thetaiotaomicron recA mutant: transfer of Bacteroides integrated conjugative elements is RecA independent
    A J Cooper
    Department of Microbiology, University of Illinois, Urbana 61801, USA
    J Bacteriol 179:6221-7. 1997
    ..because no RecA-deficient Bacteroides strain was available. We report here that the excision and integration of the Bacteroides conjugative transposons, as well as NBU1 and Tn4351, were unaffected by the absence of RecA activity...
  94. pmc The integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens is related to temperate bacteriophages
    F Boccard
    Laboratoire de Biologie et Génétique Moléculaire, URA CNRS D0086, Universite Paris Sud, Orsay, France
    EMBO J 8:973-80. 1989
    ..Sequences partially similar to pSAM2 int gene are found near the chromosomal integration zone in both S.ambofaciens strains. The possible origin of pSAM2, an element carrying plasmid as well as phage features, is discussed...
  95. ncbi High frequency transformation of the Amphotericin-producing bacterium Streptomyces nodosus
    Jasmina Nikodinovic
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, 2052 NSW, Sydney, Australia
    J Microbiol Methods 55:273-7. 2003
    ..This methodology will enable genetic and biochemical analysis of the gene cluster responsible for making Amphotericin B...
  96. pmc Characterization of a Na+/H+ antiporter gene of Escherichia coli
    E B Goldberg
    Proc Natl Acad Sci U S A 84:2615-9. 1987
    ..A wild-type gene located within 2 kilobase pairs counterclockwise of rpsT increases the Na+/H+ antiport activity when present in multiple copies...
  97. ncbi Mode and origin of replication of pSAM2, a conjugative integrating element of Streptomyces ambofaciens
    J Hagege
    Institut de Genetique et Microbiologie, URA CNRS 1354, Batiment 400, Universite Paris Sud, Orsay, France
    Mol Microbiol 10:799-812. 1993
    ..These scm contain ori and extend on both sides of the putative nick site. Sequences at the junction points of these scm are heterogeneous but short direct repeats were always found in the vicinity of these junctions...
  98. pmc Klebsiella pneumoniae multiresistance plasmid pMET1: similarity with the Yersinia pestis plasmid pCRY and integrative conjugative elements
    Alfonso J C Soler Bistué
    Center for Applied Biotechnology Studies, Department of Biological Science, College of Natural Science and Mathematics, California State University Fullerton, Fullerton, California, United States of America
    PLoS ONE 3:e1800. 2008
    ..Dissemination of antimicrobial resistance genes has become an important public health and biodefense threat. Plasmids are important contributors to the rapid acquisition of antibiotic resistance by pathogenic bacteria...
  99. pmc Thin pilus PilV adhesins of plasmid R64 recognize specific structures of the lipopolysaccharide molecules of recipient cells
    Akiko Ishiwa
    Department of Biology, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192 0397, Japan
    J Bacteriol 185:5192-9. 2003
    ..We further propose that the GlcNAc(beta1-7)Hep and Glc(alpha1-2)Glc structures of the LPS core of E. coli K-12 function as receptors for the PilVC and PilVC' adhesins, respectively...
  100. pmc Relaxed specificity of the R1162 nickase: a model for evolution of a system for conjugative mobilization of plasmids
    Eric C Becker
    Section of Molecular Genetics and Microbiology, Institute for Cellular and Molecular Biology, School of Biology, University of Texas at Austin, Austin, Texas 78712, USA
    J Bacteriol 185:3538-46. 2003
    ..Along with the structure of the oriTs themselves, these characteristics suggest a model for the evolution of this group of transfer systems...
  101. ncbi SXT-related integrating conjugative element and IncC plasmids in Vibrio cholerae O1 strains in Eastern Africa
    N Pugliese
    Dipartimento di Genetica e Microbiologia, Universita di Bari, Bari, Italy
    J Antimicrob Chemother 63:438-42. 2009
    ..The objective of this study was to investigate the extent of resistance patterns and associated mobile genetic elements in epidemic V. cholerae O1 El Tor strains isolated from Eastern Africa in the late 1990s...

Research Grants41

  1. STRUCTURAL STUDIES OF NUCLEOPROTEIN COMPLEXES
    Edward H Egelman; Fiscal Year: 2012
    ..And other complexes are involved in the assembly of viruses, including one that causes leukemia. ..
  2. FASEB CONFERENCE: HELICASES: STRUCTURE, FUNCTION
    Smita Patel; Fiscal Year: 2003
    ..This is the second FASEB meeting on this subject and only the fourth meeting dedicated to helicases since discovery of the first helicase about 25 years ago. ..
  3. FASEB Summer Conference on Helicase and NTP-Driven Nucleic Acid Motors: Structure
    Timothy Lohman; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  4. Delivering drugs via bacterial secretion systems
    Marcin S Filutowicz; Fiscal Year: 2010
    ..Here we propose to identify molecules belonging to a new category of antimicrobial compounds and to test a novel method for delivering antimicrobials, specifically, to target bacteria. ..
  5. GENETIC EXCHANGE BY A BACTERIOPHAGE FROM BARTONELLA
    MICHAEL MINNICK; Fiscal Year: 1999
    ..The proposed research will generate valuable insight into a genetic exchange mechanism in a group of important human pathogens, possibly provide clues regarding the emergence of five Bartonella species. ..
  6. CELL-PLASMID INTERACTIONS IN BACTERIAL CONJUGATION
    Philip Silverman; Fiscal Year: 1991
    ..Insofar as plasmids contribute to bacterial pathogenicity, virulence, and antibiotic resistance, studies of these interactions are directly related to human health...
  7. GENETIC DETERMINANTS OF VIRULENCE IN HELICOBACTER
    Lucy Tompkins; Fiscal Year: 1993
    ....
  8. Amersham Typhoon 9410 Variable Model Imager
    J Michael McCaffery; Fiscal Year: 2005
    ....
  9. CONJUGATIVE MOBILIZATION OF PLASMID R1162
    Richard Meyer; Fiscal Year: 2003
    ..How pSCl0l has become independent of these proteins will investigated. Overall, these studies will provide insight into how the relaxosome directs plasmid DNA to the conjugal pore for transfer. ..
  10. Tracking DNA transfer in oral biofilms
    DOMINIQUE GALLI; Fiscal Year: 2007
    ..Such information could potentially aid in developing strategies that target conjugation to minimize the spread and break the resistance cycle. [unreadable] [unreadable] [unreadable]..
  11. Tumor Cell Gene Correction by Bacterial Conjugation
    VIRGINA WATERS; Fiscal Year: 2005
    ..These experiments will provide good preparation for the development of bacterial conjugative transfer designed to correct defective genes in vivo. ..
  12. Molecular Analysis of Tral IE. Coli Helicase 1) Function
    Joel F Schildbach; Fiscal Year: 2010
    ..The proposed experiments will provide a better understanding of the mechanism of conjugation, eventually allowing manipulations designed to improve human health. ..
  13. PLASMID BIOLOGY OF ACTINOBACILLUS ACTINOMYCETEMCOMITANS
    DOMINIQUE GALLI; Fiscal Year: 2003
    ..abstract_text> ..
  14. Enzymatic Mechanisms of DNA Helicases
    Steven W Matson; Fiscal Year: 2010
    ..It is likely that other diseases will be linked with defects in DNA helicases as more disease genes are identified placing added emphasis on the importance of understanding this class of proteins. ..
  15. Chromosome loss in the fungal pathogen C. albicans
    Alexander Johnson; Fiscal Year: 2008
    ..This will also allow recombination events that take place during the parasexual cycle of C. albicans to be identified. ..
  16. pilE mRNA Analysis in Gonococci
    Stuart Hill; Fiscal Year: 2007
    ..These studies should provide unique opportunities for the development of novel pharmaceuticals for therapeutic intervention into gonorrheal or meningococcal disease. [unreadable] [unreadable] [unreadable] [unreadable]..
  17. Identification of Tetrahymena DNA Rearrangement Genes
    DOUGLAS LEE CHALKER; Fiscal Year: 2010
    ..abstract_text> ..
  18. Environmental Bioinorganic Chemistry 2008 Gordon Research Conference
    Anne O Summers; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  19. INTERACTIONS BETWEEN AGROBACTERIUM AND HOST PLANTS
    Stephen C Winans; Fiscal Year: 2010
    ..Direct targets of YenR and YenS will be identified and assessed for their roles in the biology and ecology of Y. enterocolitica and Y. pestis. ..
  20. Developmental regulation of DNA replication during programmed gene ampplification
    Geoffrey Kapler; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  21. CONJUGATION AND RECOMBINATION IN MYCOBACTERIA
    KEITH DERBYSHIRE; Fiscal Year: 2009
    ..2. To identify and characterize trans-acting transfer functions in both donor and recipient cells. 3. To examine transfer of chromosomal and plasmid DNA among fast- and slow-growing mycobacteria. ..
  22. CONJUGAL TRANSFER OF BACTERIODES ANTIBIOTIC RESISTANCES
    Abigail A Salyers; Fiscal Year: 2010
    ....
  23. A DECONVOLUTION MICROSCOPE FOR CELL BIOLOGICAL RESEARCH
    Mark Rose; Fiscal Year: 2001
    ..By these means, the digital deconvolution microscope will provide outstanding research and educational opportunities on one of the most powerful optical microscopes available for use in the life sciences. ..
  24. A BACTERIAL GROUP II INTRON INVOLVED IN CONJUGATION
    Gary Dunny; Fiscal Year: 2002
    ..Identification and analysis of small molecule splicing inhibitors using a splicing-dependent conjugation assay. ..
  25. Genome Sequencing of Toxoplasma gondii
    Ian Paulsen; Fiscal Year: 2002
    ..gondii research community is solidly behind this effort, and the history of this group (in supporting and successfully exploiting the T. gondii EST project, for example) bodes well for successful utilization of the genomic sequence...
  26. CATABOLISM OF POLYSACCHARIDES BY COLONIC BACTEROIDES
    Abigail Salyers; Fiscal Year: 2003
    ..We will continue to provide mutants for this group to test. We are interested in testing the hypothesis that mucin degradation may be the trigger for the colonization-associated change in mucosal cells. ..
  27. EVOLUTION OF DNA UPTAKE IN HAEMOPHILUS AND NEISSERIA
    ROSEMARY REDFIELD; Fiscal Year: 2003
    ..If a protein can be identified, it will be sequenced. Attempts will also be made to determine whether the USS are protected in nucleoids. ..
  28. GENETICS OF CENTRIN AND THE SPINDLE POLE BODY IN YEAST
    Mark Rose; Fiscal Year: 2003
    ..The MTOC proteins identified here may be important targets for anti-mitotic drugs for the treatment of cancer and fungal infections. ..
  29. Molecular Evolution of Enterotoxigenic B fragilis
    AUGUSTO FRANCO MORA; Fiscal Year: 2004
    ..Our results will advance our understanding of the molecular pathogenesis of ETBF infections and will serve to delineate how ETBF originated during evolution. ..
  30. Role of Enterococcus faecalis biofilms during infection
    CHRISTOPHER KRISTICH; Fiscal Year: 2004
    ..abstract_text> ..
  31. Mechanism of Conjugative DNA Transfer
    STEVEN MATSON; Fiscal Year: 2005
    ..Taken together, the results gained from these experiments will advance our understanding of the mechanism of conjugative DNA transfer and will pave the way for future experiments to look at transfer across the cell membrane. ..
  32. Mouse model of human diseases caused by mtDNA mutations
    MICHAEL KOOB; Fiscal Year: 2006
    ....
  33. Molecular analysis of the genes involved in SCA8 ataxia
    MICHAEL KOOB; Fiscal Year: 2005
    ....
  34. BUILDING RESEARCH CAPACITY IN THE SCIENCES
    Veronica Nwosu; Fiscal Year: 2006
    ..abstract_text> ..
  35. Zeiss LSM 510 Meta Confocal Microsope
    Mark Rose; Fiscal Year: 2006
    ..Study of each of these biological systems will provide valuable information pertaining to significant human health problems such as birth defects, viral infection and cancer. [unreadable] [unreadable] [unreadable]..
  36. Cell Wall Components' Role in Enterococcal Endocarditis
    Gary Dunny; Fiscal Year: 2006
    ..faecalis virulence. 2) Determine the functional domains of Asc10 that confer the activities identified in Aim 1. 3) Examine the expression of bacterial and host genes during the course of infections. ..
  37. MOLECULAR ANALYSIS OF THE INSERTION ELEMENT IS903
    KEITH DERBYSHIRE; Fiscal Year: 2004
    ..3. To establish biochemical assays that will identify specific transposase-DNA contacts, define stable domains of the protein and facilitate the development of an in vitro transposition system. ..
  38. Congugal DNA transfer into M. tuberculosis
    KEITH DERBYSHIRE; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..