cosmids

Summary

Summary: Plasmids containing at least one cos (cohesive-end site) of PHAGE LAMBDA. They are used as cloning vehicles.

Top Publications

  1. ncbi The pregnancy-specific glycoprotein (PSG) gene cluster on human chromosome 19: fine structure of the 11 PSG genes and identification of 6 new genes forming a third subgroup within the carcinoembryonic antigen (CEA) family
    S Teglund
    Department of Immunology, University of Umea, Sweden
    Genomics 23:669-84. 1994
  2. ncbi Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and 70 genes or DNA markers
    B Trask
    Human Genome Center, Lawrence Livermore National Laboratory, Livermore, California 94550
    Genomics 15:133-45. 1993
  3. pmc Characterization of chromosome ends in the filamentous fungus Neurospora crassa
    Cheng Wu
    Department of Environmental and Biomolecular Systems, Oregon Health and Science University, Beaverton, Oregon 97006, USA
    Genetics 181:1129-45. 2009
  4. pmc Construction of varicella-zoster virus recombinants from parent Oka cosmids and demonstration that ORF65 protein is dispensable for infection of human skin and T cells in the SCID-hu mouse model
    Takahiro Niizuma
    Department of Pediatrics, Stanford University School of Medicine, Stanford, California 94305, USA
    J Virol 77:6062-5. 2003
  5. pmc Defined large-scale alterations of the human cytomegalovirus genome constructed by cotransfection of overlapping cosmids
    G Kemble
    Aviron, Mountain View, California 94043, USA
    J Virol 70:2044-8. 1996
  6. ncbi Sequence-ready 1-Mb YAC, BAC and cosmid contigs covering the distal imprinted region of mouse chromosome 7
    R Kato
    Department of Integrated Genetics, National Institute of Genetics and Graduate University for Advanced Studies, Mishima, Shizuoka, Japan
    DNA Res 6:401-5. 1999
  7. pmc An automated hydrodynamic process for controlled, unbiased DNA shearing
    Y R Thorstenson
    Stanford DNA Sequencing and Technology Center, Palo Alto, California 94304, USA
    Genome Res 8:848-55. 1998
  8. ncbi Duplication of a gene-rich cluster between 16p11.1 and Xq28: a novel pericentromeric-directed mechanism for paralogous genome evolution
    E E Eichler
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Hum Mol Genet 5:899-912. 1996
  9. pmc Construction of avian adenovirus CELO recombinants in cosmids
    A Francois
    Unité de Biologie Moléculaire, INRA, Jouy en Josas, France
    J Virol 75:5288-301. 2001
  10. pmc Gene arrays at Pneumocystis carinii telomeres
    Scott P Keely
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, Cincinnati, Ohio 45267, USA
    Genetics 170:1589-600. 2005

Research Grants

  1. Essential Cell Cycle Mechanisms in Toxoplasma
    Michael W White; Fiscal Year: 2013
  2. Mycobacterial Genes Responsible for Regulating Autophagy
    Sunhee Lee; Fiscal Year: 2012
  3. Mapping the first half of the REFERENCE human binary protein interactome
    Marc Vidal; Fiscal Year: 2013
  4. DOSAGE COMPENSATION IN DROSOPHILA
    Bruce Baker; Fiscal Year: 1999
  5. Forward genetic screens for identifying antimalarial resistance genes
    Manoj T Duraisingh; Fiscal Year: 2010
  6. Mosquitocidal Avtive Clostridium Toxins
    Sarjeet Gill; Fiscal Year: 2007
  7. STRUCTURAL AND MUTATIONAL ANALYSIS OF THE LAMA3 GENE
    Angela Christiano; Fiscal Year: 1999
  8. MOLECULAR GENETIC ANALYSIS OF XQ28
    Stephen Warren; Fiscal Year: 1992
  9. DNA SEQUENCE OF STAPHYLOCOCCUS AUREUS 8325 GENOME
    JOHN LANDOLO; Fiscal Year: 1999
  10. GENETIC STUDIES OF HUMAN CELLS
    BARBARA MIGEON; Fiscal Year: 2001

Detail Information

Publications257 found, 100 shown here

  1. ncbi The pregnancy-specific glycoprotein (PSG) gene cluster on human chromosome 19: fine structure of the 11 PSG genes and identification of 6 new genes forming a third subgroup within the carcinoembryonic antigen (CEA) family
    S Teglund
    Department of Immunology, University of Umea, Sweden
    Genomics 23:669-84. 1994
    ..Unlike any of the known CEA family genes, however, they seem to lack the exon for an IgV-like N-terminal domain...
  2. ncbi Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and 70 genes or DNA markers
    B Trask
    Human Genome Center, Lawrence Livermore National Laboratory, Livermore, California 94550
    Genomics 15:133-45. 1993
    We report here the band location of 540 cosmids mapped to chromosome 19. The cosmids were mapped by fluorescence in situ hybridization (FISH) relative to chromosomal bands produced by DAPI/actinomycin staining...
  3. pmc Characterization of chromosome ends in the filamentous fungus Neurospora crassa
    Cheng Wu
    Department of Environmental and Biomolecular Systems, Oregon Health and Science University, Beaverton, Oregon 97006, USA
    Genetics 181:1129-45. 2009
    ..Finally, despite the absence of many features typically found in the telomere regions of other organisms, the Neurospora chromosome termini still retain the dynamic nature that is characteristic of chromosome ends...
  4. pmc Construction of varicella-zoster virus recombinants from parent Oka cosmids and demonstration that ORF65 protein is dispensable for infection of human skin and T cells in the SCID-hu mouse model
    Takahiro Niizuma
    Department of Pediatrics, Stanford University School of Medicine, Stanford, California 94305, USA
    J Virol 77:6062-5. 2003
    ..Using the SCID-hu mouse model, we demonstrated that the ORF65 protein is dispensable for viral replication in skin and T cells, which are critical host cell targets during primary varicella-zoster virus infection...
  5. pmc Defined large-scale alterations of the human cytomegalovirus genome constructed by cotransfection of overlapping cosmids
    G Kemble
    Aviron, Mountain View, California 94043, USA
    J Virol 70:2044-8. 1996
    ..To date, CMV is the largest virus and requires the greatest number of cosmids to be regenerated via overlapping cosmid cotransfection.
  6. ncbi Sequence-ready 1-Mb YAC, BAC and cosmid contigs covering the distal imprinted region of mouse chromosome 7
    R Kato
    Department of Integrated Genetics, National Institute of Genetics and Graduate University for Advanced Studies, Mishima, Shizuoka, Japan
    DNA Res 6:401-5. 1999
    ....
  7. pmc An automated hydrodynamic process for controlled, unbiased DNA shearing
    Y R Thorstenson
    Stanford DNA Sequencing and Technology Center, Palo Alto, California 94304, USA
    Genome Res 8:848-55. 1998
    ..The instrument, called the Point-sink Shearer (PtS), has already been exported successfully to many other laboratories...
  8. ncbi Duplication of a gene-rich cluster between 16p11.1 and Xq28: a novel pericentromeric-directed mechanism for paralogous genome evolution
    E E Eichler
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Hum Mol Genet 5:899-912. 1996
    ..5 kb gene-rich duplication shared by human Xq28 and 16p11.1. Complete comparative sequence analysis of cosmids from both loci has revealed identical Xq28 and 16p11...
  9. pmc Construction of avian adenovirus CELO recombinants in cosmids
    A Francois
    Unité de Biologie Moléculaire, INRA, Jouy en Josas, France
    J Virol 75:5288-301. 2001
    ..In order to study this potentiality, we developed an improved method for construction of adenovirus vectors in cosmids that was used to engineer the CELO genome...
  10. pmc Gene arrays at Pneumocystis carinii telomeres
    Scott P Keely
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, Cincinnati, Ohio 45267, USA
    Genetics 170:1589-600. 2005
    ..Long mononucleotide tracts were present in some MSR genes. These unstable sequences can be expected to suffer frequent frameshift mutations, providing P. carinii with another mechanism to generate antigen variation...
  11. ncbi Sequence scanning chicken cosmids: a methodology for genome screening
    M S Clark
    HGMP Resource Centre, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SB, UK
    Gene 227:223-30. 1999
    ..Results indicate that gene density is higher on the microchromosomes. During the scanning process an example of conserved linkage between chicken and human (12q34.2) has been demonstrated...
  12. ncbi Long-range chromosomal mapping of the carcinoembryonic antigen (CEA) gene family cluster
    J Thompson
    Institute of Immunobiology, University of Freiburg, Germany
    Genomics 12:761-72. 1992
    ..gel electrophoresis and of DNA from human/rodent somatic cell hybrids, as well as the assembly of ordered sets of cosmids for this gene region into contigs...
  13. ncbi An integrated physical map of 210 markers assigned to the short arm of human chromosome 11
    E Redeker
    Institute of Human Genetics, University of Amsterdam Academic Medical Centre, The Netherlands
    Genomics 21:538-50. 1994
    ..A detailed 10-Mb pulsed-field physical map of the region 11p15.3-pter was constructed. These three different approaches enabled the high-resolution mapping of 210 markers, including 22 known genes...
  14. ncbi Physical location of the human immunoglobulin lambda-like genes, 14.1, 16.1, and 16.2
    T R Bauer
    University of Miami School of Medicine, Department of Microbiology and Immunology, FL 33101
    Immunogenetics 38:387-99. 1993
    ..Consistent with the GenBank nomenclature, these human IGLL genes will be referred to as IGLL1 (14.1), IGLL2 (16.2), and IGLL3 (16.1), reflecting their position on chromosome 22, as established by this report...
  15. ncbi Identification of V1R-like putative pheromone receptor sequences in non-human primates. Characterization of V1R pseudogenes in marmoset, a primate species that possesses an intact vomeronasal organ
    Dominique Giorgi
    IGH, CNRS UPR 1142, Montpellier, France
    Chem Senses 27:529-37. 2002
    ..These observations raise the possibility that the V1R family has evolved in such a manner in mammals that every species that relies on a VNO-mediated sensory function possesses its own set of functional vomeronasal genes...
  16. pmc Metabolism of dibenzothiophene and naphthalene in Pseudomonas strains: complete DNA sequence of an upper naphthalene catabolic pathway
    S A Denome
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks
    J Bacteriol 175:6890-901. 1993
    ..Our findings indicate that a single genetic pathway controls the metabolism of dibenzothiophene, naphthalene, and phenanthrene in strain C18 and that the DOX sequence encodes a complete upper naphthalene catabolic pathway similar to NAH...
  17. pmc Cloning and molecular analysis of genes affecting expression of binding substance, the recipient-encoded receptor(s) mediating mating aggregate formation in Enterococcus faecalis
    B A Bensing
    Institute for Advanced Studies in Biological Process Technology, University of Minnesota, St Paul 55108
    J Bacteriol 175:7421-9. 1993
    ..The ebsA and ebsB genes encode peptides likely to function in cell wall metabolism, whereas ebsC may encode a product that suppresses the function or expression of EbsB...
  18. ncbi Multiple TL-like loci in the grc-G/C region of the rat
    M J Kirisits
    Department of Pathology, University of Pittsburgh School of Medicine, PA 15261
    Immunogenetics 39:301-15. 1994
    ..Several cosmids spanning approximately 45 kilobases of this region were analyzed for their class I loci, using a mouse general ..
  19. ncbi Organization of the hydrogenase gene cluster from Bradyrhizobium japonicum: sequences and analysis of five more hydrogenase-related genes
    C Fu
    Department of Biology, Johns Hopkins University, Baltimore, MD 21218
    Gene 145:91-6. 1994
    ..The latter proteins contain two 'C-X-X-C' motifs, which may serve as iron ligands for non-heme iron proteins involved as intermediate electron carriers or in the assembly process for Fe-S (or NiFe-S) clusters...
  20. ncbi Structural analysis of the mouse T-cell receptor Tcra V2 subfamily
    K Wang
    Department of Molecular Biotechnology GJ 10, University of Washington, Seattle 98195
    Immunogenetics 40:116-22. 1994
    ..PL and PL/J mouse strains with a Tcra V2 subfamily-specific probe. These V gene segment polymorphisms may cause the differential Tcra V gene usage in induced experimental allergic encephalomyelitis between B10.PL and PL/J mice...
  21. ncbi The Mycobacterium leprae genome: systematic sequence analysis identifies key catabolic enzymes, ATP-dependent transport systems and a novel polA locus associated with genomic variability
    H Fsihi
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Mol Microbiol 16:909-19. 1995
    ..leprae. This is the first evidence for genomic variability in the leprosy bacillus and might ultimately be useful for developing a molecular test capable of distinguishing between strains of M. leprae...
  22. ncbi The blasticidin S biosynthesis gene cluster from Streptomyces griseochromogenes: sequence analysis, organization, and initial characterization
    Martha C Cone
    Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR 97331, USA
    Chembiochem 4:821-8. 2003
    ..Additionally, the gene that directs the production of free cytosine, blsM, was subcloned and expressed in Escherichia coli. Characterization of BlsM revealed that cytidine monophosphate serves as the precursor to cytosine...
  23. pmc Mutation of the principal sigma factor causes loss of virulence in a strain of the Mycobacterium tuberculosis complex
    D M Collins
    AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand
    Proc Natl Acad Sci U S A 92:8036-40. 1995
    ..Study of genes and proteins differentially regulated by the mutant transcription factor should facilitate identification of further virulence factors...
  24. ncbi Gene cluster of Arthrobacter ilicis Ru61a involved in the degradation of quinaldine to anthranilate: characterization and functional expression of the quinaldine 4-oxidase qoxLMS genes
    Katja Parschat
    AG Mikrobiologie, Institut für Chemie und Biologie des Meeres, Carl von Ossietzky Universitat Oldenburg, D 26111 Oldenburg, Germany
    J Biol Chem 278:27483-94. 2003
    ..Although Escherichia coli clones containing the recombinant cosmid did not transform quinaldine, cosmids harboring the 23-kb region, or a 10...
  25. ncbi Limited regions of homology between linear and circular plasmids encoding methylenomycin biosynthesis in two independently isolated streptomycetes
    Masayuki Yamasaki
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi Hiroshima 739 8530, Japan
    Microbiology 149:1351-6. 2003
    ..It is likely that the mmy gene cluster was horizontally transferred as a set together with the parAB region in the comparatively recent evolutionary past...
  26. pmc Isolation and characterization of a MAGE gene family in the Xp21.3 region
    F Muscatelli
    Imperial Cancer Research Fund Laboratories, John Radcliffe Hospital, Oxford, United Kingdom
    Proc Natl Acad Sci U S A 92:4987-91. 1995
    ..with strong homology to the MAGE gene family located in Xq27-qter has been isolated by using exon-trapping of cosmids in the Xp21.3 region...
  27. pmc Recombinant environmental libraries provide access to microbial diversity for drug discovery from natural products
    Sophie Courtois
    Laboratoire d Ecologie Microbienne du Sol, UMR CNRS 5557, Universite Claude Bernard Lyon 1, 69622 Villeurbanne Cedex, France
    Appl Environ Microbiol 69:49-55. 2003
    ....
  28. ncbi Cloning and molecular analysis of the Salmonella enterica ansP gene, encoding an L-asparagine permease
    M P Jennings
    Division of Science and Technology, Griffith University, Nathan, Queensland, Australia
    Microbiology 141:141-6. 1995
    ..Strains containing the cloned ansP gene demonstrated a many-fold increase in L-asparagine uptake in comparison with control strains...
  29. pmc A molecular map of the chicken major histocompatibility complex: the class II beta genes are closely linked to the class I genes and the nucleolar organizer
    F Guillemot
    Institut d embryologie du CNRS et du Collège de France, Nogent sur Marne
    EMBO J 7:2775-85. 1988
    ..is by far the most divergent known member of the class I gene family, hybridized to six B-F genes present in the cosmids. One of the clusters was shown to contain two rRNA transcriptional units from the nucleolar organizer region (NOR)..
  30. ncbi Genomic scanning for expressed sequences in Xp21 identifies the glycerol kinase gene
    W Guo
    Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030
    Nat Genet 4:367-72. 1993
    ..Independent confirmation of the identity of this gene was obtained by functional complementation of GK deficient E. coli mutants with a construct containing the complete human X-linked GK coding sequence...
  31. pmc Cloning and promoter identification of the iron-regulated cir gene of Escherichia coli
    D W Griggs
    Department of Microbiology, University of Illinois, Urbana 61801
    J Bacteriol 169:5343-52. 1987
    ....
  32. ncbi Putative X-linked adrenoleukodystrophy gene shares unexpected homology with ABC transporters
    J Mosser
    Laboratoire de Genetique Moleculaire des Eucaryotes du CNRS, INSERM Unite 184, Institut de Chimie Biologique, Faculte de Medecine, Strasbourg, France
    Nature 361:726-30. 1993
    ..The deduced protein sequence shows significant sequence identity to a peroxisomal membrane protein of M(r) 70K that is involved in peroxisome biogenesis and belongs to the 'ATP-binding cassette' superfamily of transporters...
  33. ncbi Isolation of the human Xp21 glycerol kinase gene by positional cloning
    A P Walker
    ICRF Laboratories, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK
    Hum Mol Genet 2:107-14. 1993
    ..We have used a genomic exon amplification strategy to isolate potential exons from two cosmids which mapped to this interval...
  34. ncbi The rfaD locus: a region of rearrangement in Vibrio cholerae O139
    S Vimont
    INSERM U 411, Faculte de Medecine Necker Enfants Malades, Paris, France
    Gene 185:43-7. 1997
    ..cholerae O1, resulting in the emergence of V. cholerae O139. The putative source of exogenous DNA might have been V. cholerae O22, O141 and O155...
  35. ncbi Detection of type III secretion system genes in animal isolates of Bordetella bronchiseptica
    C Winstanley
    Department of Medical Microbiology and Genito Urinary Medicine, University of Liverpool, P O Box 147, Liverpool, UK
    Vet Microbiol 72:329-37. 2000
    ..bronchiseptica, it is likely that different clinical manifestations may be due to variations in gene expression or host factors, rather than the absence or presence of TTS genes...
  36. ncbi One-megabase sequence analysis of the human immunoglobulin lambda gene locus
    K Kawasaki
    Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan
    Genome Res 7:250-61. 1997
    ..Sequence organization suggests that large DNA duplications diversified the germ-line repertoire of the V lambda gene segments...
  37. ncbi Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene
    G Volckaert
    Katholieke Universiteit Leuven, Laboratory of Gene Technology, Belgium
    Yeast 13:251-9. 1997
    ..The remaining five ORFs do not encode known functions, but three of these show weak to high similarities to other ORFs in the Saccharomyces cerevisiae genome and one (YGR280c) codes for a particularly lysine-rich protein...
  38. ncbi A novel valanimycin-resistance determinant (vlmF) from Streptomyces viridifaciens MG456-hF10
    Y Ma
    Department of Chemistry, Rice University, Houston, TX 77005 1892, USA
    Microbiology 146:345-52. 2000
    ....
  39. ncbi Complete genomic structure DNA polymorphisms, and alternative splicing of the human AF-6 gene
    S Saito
    Center for Molecular Biology and Cytogenetics, SRL, Inc, Tokyo, Japan
    DNA Res 5:115-20. 1998
    ..These results may contribute to an understanding of the mechanism causing chromosomal translocations in leukemic cells...
  40. ncbi Isolation of coding sequences from bovine cosmids by means of exon trapping
    S Comincini
    Istituto per la Difesa e la Valorizzazione del Germoplasma Animale I D V G A C N R, Via Celoria 10, 20133 Milano, Italy
    Mamm Genome 8:486-90. 1997
    Exon trapping was employed to identify coding sequences from a collection of 46 bovine cosmids, previously characterized for the presence of microsatellite markers and physically mapped to chromosomes by FISH...
  41. ncbi Physical mapping 220 kb centromeric of the human MHC and DNA sequence analysis of the 43-kb segment including the RING1, HKE6, and HKE4 genes
    Y Y Kikuti
    Department of Transplantation Immunology, Tokai University School of Medicine, Isehara, Kanagawa, Japan
    Genomics 42:422-35. 1997
    ..2 Alu per kb), and no gene has been identified in this so far. The region is equivalent to part of the mouse t complex and could be of relevance to human development...
  42. ncbi A new inward rectifier potassium channel gene (KCNJ15) localized on chromosome 21 in the Down syndrome chromosome region 1 (DCR1)
    P Gosset
    Faculte de Medecine Necker, CNRS URA1335, 156 rue de Vaugirard, Paris, 75015, France
    Genomics 44:237-41. 1997
    ..Isolation, mapping, and sequencing of trapped exons and captured cDNAs from cosmids of this region have revealed the presence of a gene (KCNJ15) encoding a potassium (K+) channel belonging to the ..
  43. ncbi Evidence for the presence in Burkholderia pseudomallei of a type III secretion system-associated gene cluster
    C Winstanley
    Department of Biomedical Sciences, University of Bradford, West Yorkshire
    J Med Microbiol 48:649-56. 1999
    ..solanacearum proteins. The presence of type III secretion-associated genes in B. pseudomallei pathogens suggests a possible role for type III secretion systems in the pathogenicity of this organism...
  44. pmc Identification of the novobiocin biosynthetic gene cluster of Streptomyces spheroides NCIB 11891
    M Steffensky
    Pharmazeutische Biologie, Pharmazeutisches Institut, Eberhard Karls Universitat Tubingen, 72076 Tubingen, Germany
    Antimicrob Agents Chemother 44:1214-22. 2000
    ..Heterologous expression of a key enzyme of novobiocin biosynthesis, i.e., novobiocic acid synthetase, in Streptomyces lividans TK24 further confirmed the involvement of the analyzed genes in the biosynthesis of the antibiotic...
  45. ncbi Isolation and mapping of RAB2L, a human cDNA that encodes a protein homologous to RalGDS
    M Isomura
    Laboratory of Molecular Medicine, University of Tokyo, Japan
    Cytogenet Cell Genet 74:263-5. 1996
    ..Northern analysis revealed that the gene RAB2L is expressed in all human tissues examined. We assigned this gene locus to chromosome band 6p21.3 by fluorescence in situ hybridization...
  46. pmc Identification and overexpression in Escherichia coli of a Mycobacterium leprae gene, pon1, encoding a high-molecular-mass class A penicillin-binding protein, PBP1
    J Basu
    Department of Chemistry, Bose Institute, Calcutta, India
    J Bacteriol 178:1707-11. 1996
    ..The solubilized PBP1 derivatives can be purified by affinity chromatography on Ni2+-nitrilotriacetic acid agarose. They have low affinities for the usual penicillins and cephalosporins...
  47. ncbi Molecular cloning and characterization of the Fugu rubripes MEST/COPG2 imprinting cluster and chromosomal localization in Fugu and Tetraodon nigroviridis
    B Brunner
    Human Genetics, University Hospital Nijmegen, The Netherlands
    Chromosome Res 8:465-76. 2000
    ....
  48. ncbi Isolation and characterization of a human cDNA clone (GCN5L1) homologous to GCN5, a yeast transcription activator
    M Inoue
    Laboratory of Molecular Medicine, University of Tokyo, Japan
    Cytogenet Cell Genet 73:134-6. 1996
    ..We isolated a genomic clone corresponding to this cDNA from a human cosmid library and mapped it to chromosome 12q13 --> q14 by fluorescent in situ hybridization (FISH)...
  49. ncbi The human lanosterol synthase gene maps to chromosome 21q22.3
    M Young
    Laboratory of Human Molecular Genetics, Department of Genetics and Microbiology, University of Geneva, Switzerland
    Hum Genet 97:620-4. 1996
    ..Using pools of random HC21-specific cosmids from the LL21NC02-Q library and cosmids from 21q22...
  50. ncbi Structure of the human zinc finger protein HIVEP3: molecular cloning, expression, exon-intron structure, and comparison with paralogous genes HIVEP1 and HIVEP2
    M D Hicar
    Department of Molecular Virology, Immunology, and Medical Genetics, College of Medicine and Public Health, The Ohio State University, Columbus, Ohio 43210, USA
    Genomics 71:89-100. 2001
    ....
  51. ncbi Positional cloning of the mouse retrovirus restriction gene Fv1
    S Best
    Division of Virology, National Institute for Medical Research, London, UK
    Nature 382:826-9. 1996
    ....
  52. pmc Cloning and characterization of nanB, a second Streptococcus pneumoniae neuraminidase gene, and purification of the NanB enzyme from recombinant Escherichia coli
    A M Berry
    Molecular Microbiology Unit, Women s and Children s Hospital, North Adelaide, South Australia, Australia
    J Bacteriol 178:4854-60. 1996
    ..The discrepancy between this estimate and the size predicted from the nucleotide sequence is most likely a consequence of C-terminal processing or anomalous electrophoretic behavior...
  53. ncbi hilA is a novel ompR/toxR family member that activates the expression of Salmonella typhimurium invasion genes
    V Bajaj
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Microbiol 18:715-27. 1995
    ..Our results are consistent with the hypothesis that hilA plays a key role in the regulation of Salmonella invasion during infection...
  54. ncbi Cloning, expression and chromosome mapping of adducin-like 70 (ADDL), a human cDNA highly homologous to human erythrocyte adducin
    T Katagiri
    Otsuka GEN Research Institute, Otsuka Pharmaceutical Co, Ltd, Tokushima, Japan
    Cytogenet Cell Genet 74:90-5. 1996
    ..Northern blot analysis indicated ubiquitous expression of this gene in adult human tissues. We localized the gene to chromosome bands 10q24.2-->q24.3 by fluorescence in situ hybridization (FISH)...
  55. ncbi Gene arrangement and organization in a approximately 76 kb fragment encompassing the oriC region of the chromosome of Mycobacterium leprae
    H Fsihi
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Microbiology 142:3147-61. 1996
    ..Among the many other noteworthy features were ORFs encoding putative serine/threonine protein kinases and a protein phosphatase, three tRNA genes, one M. leprae-specific repetitive element and a glnQ pseudogene...
  56. ncbi Sequence of the Corynebacterium glutamicum pyruvate carboxylase gene
    M A Koffas
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139, USA
    Appl Microbiol Biotechnol 50:346-52. 1998
    ..This 850-base-pair fragment was used to probe a C. glutamicum cosmid library and four candidate pc cosmids were identified...
  57. ncbi Identification and characterization of a new gene in the human Xq28 region
    A M van den Ouweland
    Department of Human Genetics, University Hospital of Nijmegen, The Netherlands
    Hum Mol Genet 1:269-73. 1992
    ..The genomic sequence shows a 100% homology with the recently identified QM cDNA sequence...
  58. ncbi Cloning and sequence analysis of the fermentative alcohol-dehydrogenase-encoding gene of Escherichia coli
    P E Goodlove
    Department of Microbiology, Southern Illinois University, Carbondale 62901
    Gene 85:209-14. 1989
    ..Codon usage analysis indicates that adhE should be highly expressed. This gene shows no significant homology to any previously sequenced ADH-encoding gene...
  59. ncbi Expressed genes, Alu repeats and polymorphisms in cosmids sequenced from chromosome 4p16.3
    W R McCombie
    Section of Receptor Biochemistry and Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892
    Nat Genet 1:348-53. 1992
    The sequences of three cosmids (90 kilobases) from the Huntington's disease region in chromosome 4p16.3 have been determined...
  60. ncbi A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1: purification, properties, genetic cloning and expression in Escherichia coli
    Jian Feng Wu
    Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China
    Arch Microbiol 183:1-8. 2005
    ..The enzyme was actively synthesized when the genes coding for the ORF9 and ORF10 were cloned into Escherichia coli...
  61. pmc The murine Spi-2 proteinase inhibitor locus: a multigene family with a hypervariable reactive site domain
    J D Inglis
    MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK
    EMBO J 10:255-61. 1991
    ..The divergence is also appreciably greater than that occurring in an adjacent non-coding sequence. This phenomenon has established novel potential inhibitory specificities, while maintaining a functional inhibitor structure...
  62. ncbi Cloning of the putative tumour suppressor gene for hereditary multiple exostoses (EXT1)
    J Ahn
    Department of Biology, University of Houston, Texas 77204 5513, USA
    Nat Genet 11:137-43. 1995
    ..However, recent studies in sporadic and exostosis-derived chondrosarcomas suggest that the 8q24.1-encoded EXT1 gene may have tumour suppressor function...
  63. ncbi Cloning and characterisation of the serC and aroA genes of Yersinia enterocolitica, and construction of an aroA mutant
    P O'Gaora
    Department of Molecular Biology, Wellcome Biotech, Beckenham, Kent, U K
    Gene 84:23-30. 1989
    ..The cloned Y. enterocolitica aroA gene was inactivated in vitro and reintroduced into the parental Y. enterocolitica 8081 strain using the suicide vector pJM703.1. Stable aroA insertion mutants of Y. enterocolitica were isolated...
  64. ncbi The coding sequence of the hemolytically inactive C4A6 allotype of human complement component C4 reveals that a single arginine to tryptophan substitution at beta-chain residue 458 is the likely cause of the defect
    M J Anderson
    Medical Research Council Immunochemistry Unit, Department of Biochemistry, Oxford, U K
    J Immunol 148:2795-802. 1992
    ..We propose that this Arg to Trp substitution at beta-chain residue 458 is responsible for the inability of C4A6 to bind C5 in the C5 convertase...
  65. ncbi Community genomics among stratified microbial assemblages in the ocean's interior
    Edward F DeLong
    Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 311:496-503. 2006
    ..Comparative genomic analyses of stratified microbial communities have the potential to provide significant insight into higher-order community organization and dynamics...
  66. ncbi Molecular cloning and characterization of a novel human gene homologous to the murine ecotropic retroviral receptor
    T Yoshimoto
    Department of Pathology, New York University Medical Center, New York 10016
    Virology 185:10-7. 1991
    ..The genomic gene was also isolated from a cosmid library derived from human lymphocytes, and its organization was elucidated. The gene mapped to human chromosome 13...
  67. ncbi Structure and sequence of the human homeobox gene HOX7
    J E Hewitt
    Department of Biochemistry and Molecular Genetics, St Mary s Hospital Medical School, Imperial College of Science, Technology, and Medicine, London, United Kingdom
    Genomics 11:670-8. 1991
    ..The region encompassing the promoter region, exon 1, and the 5' region of exon 2 have a higher than expected frequency of CpG dinucleotides; numerous sites for rare-cutter restriction enzymes are present, a characteristic of HTF islands...
  68. pmc Environmental DNA fragment conferring early and increased sporulation and antibiotic production in Streptomyces species
    Asuncion Martinez
    Sanofi Aventis Cambridge Genomics Center, Cambridge, Massachusetts, USA
    Appl Environ Microbiol 71:1638-41. 2005
    ..We show that Streptomyces lividans strains bearing rep are particularly useful as expression hosts for heterologous antibiotic production...
  69. pmc Conserved gene arrangement in the origin region of the Streptomyces coelicolor chromosome
    M J Calcutt
    Department of Biochemistry, University of Missouri Columbia 65212
    J Bacteriol 174:3220-6. 1992
    ..In related work, J. Zakrzewska-Czerwinska and H. Schrempf (J. Bacteriol. 174:2688-2693, 1992) have identified the homologous sequence from the closely-related Streptomyces lividans as capable of self-replication...
  70. ncbi The lipopeptide antibiotic A54145 biosynthetic gene cluster from Streptomyces fradiae
    Vivian Miao
    Cubist Pharmaceuticals Inc, 65 Hayden Av, Lexington, MA, 02421, USA
    J Ind Microbiol Biotechnol 33:129-40. 2006
    ..This information may contribute to the design of experiments to address both fundamental and applied questions in lipopeptide biosynthesis, engineering and drug development...
  71. ncbi Molecular isolation and characterization of an expressed gene from the human Y chromosome
    J S Zhang
    Division of Cell and Developmental Genetics, VA Medical Center, University of California, San Francisco 94121
    Hum Mol Genet 1:717-26. 1992
    ..The Y-231 (TSPY) gene is conserved in the male genome and expressed in the testis of the chimpanzee, suggesting that it may play an important role in the physiology of this organ in man and the great ape...
  72. pmc Cloning and sequencing of a gene encoding a 21-kilodalton outer membrane protein from Bordetella avium and expression of the gene in Salmonella typhimurium
    C R Gentry-Weeks
    Laboratory of Microbial Ecology, National Institute of Dental Research, Bethesda, Maryland 20892
    J Bacteriol 174:7729-42. 1992
    ..avium 197 resulted in colonization of the trachea and thymus with B. avium 197...
  73. pmc Novel swine virulence determinant in the left variable region of the African swine fever virus genome
    J G Neilan
    Plum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, New York 11944 0848, USA
    J Virol 76:3095-104. 2002
    ..Together, these results indicate that a region within the left variable region of the ASFV genome containing the MGF 360 and 530 genes represents a previously unrecognized virulence determinant for domestic swine...
  74. ncbi Structure of the murine fifth complement component (C5) gene. A large, highly interrupted gene with a variant donor splice site and organizational homology with the third and fourth complement component genes
    D L Haviland
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110
    J Biol Chem 266:11818-25. 1991
    ..The 5'-flanking region also contained sequences identical with several cis-acting motifs known to bind the liver-specific nuclear protein LF-A1 and the nuclear protein NF-kappa B.(ABSTRACT TRUNCATED AT 250 WORDS)..
  75. pmc Expression cloning and characterization of the C28 acyltransferase of lipid A biosynthesis in Rhizobium leguminosarum
    Shib Sankar Basu
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:28959-71. 2002
    ..We now report the identification of five hybrid cosmids that direct the overexpression of this activity by screening approximately 4000 lysates of individual colonies of ..
  76. pmc Cloning and nucleotide sequence of the pvdA gene encoding the pyoverdin biosynthetic enzyme L-ornithine N5-oxygenase in Pseudomonas aeruginosa
    P Visca
    Istituto di Microbiologia, Universita di Roma La Sapienza, Italy
    J Bacteriol 176:1128-40. 1994
    ..Our results suggest that omega-amino acid oxygenases have been conserved over a wide evolutionary range and probably evolved from a common ancestor...
  77. pmc Purification of recombinant Helicobacter pylori urease apoenzyme encoded by ureA and ureB
    L T Hu
    Department of Medicine, University of Maryland School of Medicine, Baltimore 21201
    Infect Immun 60:2657-66. 1992
    ..coli and that these genes alone are sufficient for the synthesis and assembly of the native size enzyme. Genes downstream of ureB, however, are necessary for production of a catalytically active urease...
  78. pmc The alkene monooxygenase from Xanthobacter strain Py2 is closely related to aromatic monooxygenases and catalyzes aromatic monohydroxylation of benzene, toluene, and phenol
    N Y Zhou
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ, United Kingdom
    Appl Environ Microbiol 65:1589-95. 1999
    ..This suggests that the inducer specificity of the alkene monooxygenase may have evolved to benefit from the naturally broad substrate specificity of this class of monooxygenase and the ability of the host strain to grow on catechol...
  79. ncbi Structural organization of the gene for the alpha 1 chain of human type IV collagen
    R Soininen
    Department of Biochemistry, University of Oulu, Finland
    J Biol Chem 264:13565-71. 1989
    ..The results demonstrate that the human alpha 1(IV) collagen gene has a structure distinctly different from the genes for fibrillar collagens and also that it is considerably larger than any collagen gene characterized to date...
  80. ncbi Thiopurine methyltransferase pharmacogenetics: human gene cloning and characterization of a common polymorphism
    C Szumlanski
    Department of Pharmacology, Mayo Medical School, Rochester, MN 55905, USA
    DNA Cell Biol 15:17-30. 1996
    ....
  81. ncbi Two copies of the human apolipoprotein C-I gene are linked closely to the apolipoprotein E gene
    S J Lauer
    Gladstone Foundation Laboratories for Cardiovascular Disease, University of California, San Francisco 94140 0608
    J Biol Chem 263:7277-86. 1988
    ..Furthermore, they may be considered to be constituents of a family of seven apolipoprotein genes (apoE, -C-I, -C-II, -C-III, -A-I, -A-II, and -A-IV) that have a common evolutionary origin...
  82. ncbi Association of polymorphisms in the HLA-B region with extended haplotypes
    G E Egea
    American Red Cross Blood Services Northeast Region, Dedham, MA 02026
    Immunogenetics 33:4-11. 1991
    ..The two DNA probes, M20A and R5A, were derived from previously cloned cosmids and are located 38 and 110 kilobases (kb) centromeric to HLA-B, respectively...
  83. pmc Cloning and characterization of bacteriophage-like DNA from Haemophilus somnus homologous to phages P2 and HP1
    R A Pontarollo
    Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada
    J Bacteriol 179:1872-9. 1997
    ..Elucidation of a potential role for this cryptic prophage in the H. somnus life cycle requires more study...
  84. pmc Molecular evidence that the H-2D and H-2L genes arose by duplication. Differences between the evolution of the class I genes in mice and humans
    R J Rubocki
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110
    J Exp Med 171:2043-61. 1990
    ..These and other recent molecular studies provide multiple examples of expansion and contraction of the class I genes in the D region.(ABSTRACT TRUNCATED AT 400 WORDS)..
  85. ncbi Cloning and characterization of the chlorophyll biosynthesis gene chlM from Synechocystis PCC 6803 by complementation of a bacteriochlorophyll biosynthesis mutant of Rhodobacter capsulatus
    C A Smith
    Department of Biology, Indiana University, Bloomington 47405, USA
    Plant Mol Biol 30:1307-14. 1996
    ..The complementing Synechocystis cosmid was also shown to code for a gene product that is a member of a highly conserved family of RNA binding proteins, the function of which in cyanobacteria remains undetermined...
  86. ncbi Physical mapping of the 5S ribosomal RNA genes in Arabidopsis thaliana by multi-color fluorescence in situ hybridization with cosmid clones
    M Murata
    Research Institute for Bioresources, Okayama University, Kurashiki, Japan
    Plant J 12:31-7. 1997
    ..8S-25S and 5S rDNAs on its short (L) arm; and/chromosome 5 is metacentric carrying a large cluster of 5S rDNA on its L arm...
  87. ncbi The human alpha-amylase multigene family consists of haplotypes with variable numbers of genes
    P C Groot
    Institute of Human Genetics, Medical Faculty, Vrije Universiteit, Amsterdam, The Netherlands
    Genomics 5:29-42. 1989
    ..From the restriction maps of the overlapping cosmids and a comparison of these maps with the restriction enzyme patterns of DNA from the donor and family members, we ..
  88. pmc Origin of the 2-amino-2-deoxy-gluconate unit in Rhizobium leguminosarum lipid A. Expression cloning of the outer membrane oxidase LpxQ
    Nanette L S Que-Gewirth
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 278:12120-9. 2003
    ..leguminosarum 3841 genomic DNA library in the host strain R. etli CE3. Two cosmids (p1S11D and p1U12G) were identified in this manner and transferred into S...
  89. ncbi Novel detection of restriction fragment length polymorphisms in the human major histocompatibility complex
    S J Cross
    Department of Biochemistry, University of Oxford, UK
    Immunogenetics 34:376-84. 1991
    ..The RFLPs detected by the cosmid clones thus provide new tools which will be useful in the further genetic analysis of the MHC class III region...
  90. pmc Cloning and expression of the Vibrio cholerae neuraminidase gene nanH in Escherichia coli
    E R Vimr
    Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana 61801
    J Bacteriol 170:1495-504. 1988
    ..The nanH gene was subcloned from one of the cosmids and was located within a 4.8-kilobase-pair BglII restriction endonuclease fragment...
  91. pmc Cloning and characterization of the ferulic acid catabolic genes of Sphingomonas paucimobilis SYK-6
    Eiji Masai
    Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata 940 2188, Japan
    Appl Environ Microbiol 68:4416-24. 2002
    ..Insertional inactivation of each fer gene in S. paucimobilis SYK-6 suggested that the ferA gene is essential and that ferB and ferB2 genes are involved in ferulic acid degradation...
  92. ncbi Characterization of type IV pilus genes in Pseudomonas syringae pv. tomato DC3000
    E Roine
    Department of Biosciences, University of Helsinki, Finland
    Mol Plant Microbe Interact 11:1048-56. 1998
    ..In addition, the expression of the DC3000 pilA gene appears to contribute to the UV tolerance of P. syringae and may play a role in survival on the plant leaf surface...
  93. ncbi Genome encyclopedias and their use for comparative analysis of Rhodobacter capsulatus strains
    M Fonstein
    Department of Molecular Genetics and Cell Biology, The University of Chicago, IL 60637, USA
    Electrophoresis 19:469-77. 1998
    ..capsulatus SB 1003 genome project started with the construction of its cosmid encyclopedia, which comprises 192 cosmids covering the chromosome and the 134 kbp plasmid in strain SB 1003, with the exact map coordinates of each cosmid...
  94. ncbi MlrA, a novel regulator of curli (AgF) and extracellular matrix synthesis by Escherichia coli and Salmonella enterica serovar Typhimurium
    P K Brown
    Department of Biology, Washington University, Saint Louis, MO 63130 2525, USA
    Mol Microbiol 41:349-63. 2001
    ..Two cosmids from a genomic library of avian pathogenic E...
  95. ncbi Cloning of the human phenol sulfotransferase gene family: three genes implicated in the metabolism of catecholamines, thyroid hormones and drugs
    T P Dooley
    Southern Research Institute, Birmingham, AL 35205, USA
    Chem Biol Interact 109:29-41. 1998
    ..Recent advances in the molecular biology of the human PST gene family are summarized...
  96. ncbi Paramyosin gene (unc-15) of Caenorhabditis elegans. Molecular cloning, nucleotide sequence and models for thick filament structure
    H Kagawa
    Department of Biology, Faculty of Science, Okayama University, Japan
    J Mol Biol 207:311-33. 1989
    ..The implications of these results for the structure of the bare zone and the assembly of nematode thick filaments are discussed...
  97. ncbi Molecular cloning of a gene for inversion of embryo turning (inv) with cystic kidney
    Toshio Mochizuki
    Department of Medicine II, Hokkaido University School of Medicine, Sapporo, Japan
    Nephrol Dial Transplant 17:68-70. 2002
    ..The gene product involves ankyrin motif repeats, indicating that a cytoskeleton disruption induces the situs inversus and the cystic kidney...
  98. ncbi Sequence and expression of human hair keratin genes
    P E Bowden
    Department of Dermatology, University of Wales College of Medicine, Heath Park, Cardiff, UK
    J Dermatol Sci 7:S152-63. 1994
    ..The coding sequences were homologous to mouse cDNA (77-88%). These human hair-specific keratin clones are useful molecular tools for studies of hair differentiation...
  99. pmc Pathogenicity island sequences of pyelonephritogenic Escherichia coli CFT073 are associated with virulent uropathogenic strains
    J S Kao
    Division of Infectious Diseases, University of Maryland School of Medicine, Baltimore 21201, USA
    Infect Immun 65:2812-20. 1997
    ..When used as probes for DNA hybridization, these sequences were found significantly more often in strains associated with the clinical syndromes of cystitis (82%) and acute pyelonephritis (79%) than in fecal strains (19%; P < 0.001)...
  100. pmc Sequence analysis of insecticidal genes from Xenorhabdus nematophilus PMFI296
    J A Morgan
    Department of Plant Pathology and Microbiology, Horticulture Research International, Wellesbourne, Warwick CV35 9EF, United Kingdom
    Appl Environ Microbiol 67:2062-9. 2001
    ..nematophilus PMFI296...
  101. pmc Identification and sequence analysis of the Rhizobium meliloti dctA gene encoding the C4-dicarboxylate carrier
    T Engelke
    Lehrstuhl fur Genetik, Fakultat fur Biologie, Universitat Bielefeld, Federal Republic of Germany
    J Bacteriol 171:5551-60. 1989
    ..6 kilodaltons, respectively. The hydrophobicity plot suggests that DctA is a membrane protein with several membrane passages. The amino acid sequences of the R. meliloti and the R. leguminosarum DctA proteins were highly conserved (82%)...

Research Grants62

  1. Essential Cell Cycle Mechanisms in Toxoplasma
    Michael W White; Fiscal Year: 2013
    ..Therefore, through this investigation of the molecular basis of the parasite cell cycle, new potential drug targets will be identified upon which novel therapies may be developed. ..
  2. Mycobacterial Genes Responsible for Regulating Autophagy
    Sunhee Lee; Fiscal Year: 2012
    ..The results of the study will be useful for improving the vaccine efficacy of BCG and developing noble TB vaccine candidates. ..
  3. Mapping the first half of the REFERENCE human binary protein interactome
    Marc Vidal; Fiscal Year: 2013
    ..data exploded for the Human Genome Sequencing project in the 1990s when four crucial elements were assembled: i) cosmids, BAC, and YAC clone resources covering most of the genome;ii) automated laser-fluorescence sequencing, iii) the ..
  4. DOSAGE COMPENSATION IN DROSOPHILA
    Bruce Baker; Fiscal Year: 1999
    ....
  5. Forward genetic screens for identifying antimalarial resistance genes
    Manoj T Duraisingh; Fiscal Year: 2010
    ..This will provide us with tools to monitor drug- resistance in the public, as well as identify targets that will aid in the development of new drugs. ..
  6. Mosquitocidal Avtive Clostridium Toxins
    Sarjeet Gill; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  7. STRUCTURAL AND MUTATIONAL ANALYSIS OF THE LAMA3 GENE
    Angela Christiano; Fiscal Year: 1999
    ....
  8. MOLECULAR GENETIC ANALYSIS OF XQ28
    Stephen Warren; Fiscal Year: 1992
    ..hybrid cells will be prepared and clones containing human inserts identified and isolated to provide a panel of cosmids of sufficient number to saturate Xq28...
  9. DNA SEQUENCE OF STAPHYLOCOCCUS AUREUS 8325 GENOME
    JOHN LANDOLO; Fiscal Year: 1999
    ..Therefore, as a single specific aim, we will sequence and annotate the 2.8 Mb genome of this important pathogen. ..
  10. GENETIC STUDIES OF HUMAN CELLS
    BARBARA MIGEON; Fiscal Year: 2001
    ..Identification of relevant sequences is facilitated by human YAC contigs and a library which includes cosmids from the region...
  11. M TUBERCULOSIS SURVIVAL IN MACROPHAGES
    Richard Friedman; Fiscal Year: 1999
    ..smegmatis clones that survive within the U937 macrophage monolayers for further study. Plasmids or cosmids from these clones will be recovered and the M...
  12. FUNCTIONAL ANALYSIS OF THE MYXOCOCCUS XANTHUS GENOME
    Philip Youderian; Fiscal Year: 2004
    ..Our efforts will show that the sequences of the genomes of most pathogenic and other interesting microbes can be completed at a much more reasonable cost than current efforts entail. ..
  13. MOLECULAR GENETIC ANALYSIS OF ANKYLOSING SPONDYLITIS
    John Reveille; Fiscal Year: 1992
    ..The identification and characterization of this "second AS gene" would not only allow subsequent analyses evaluating the product of this gene, but also give important clues as to the very genetic predisposition to AS...
  14. CHEMILUMINESCENT MULTIPLEX GENOME MAPPING
    John Fox; Fiscal Year: 1992
    ..This data will be useful for positioning cloned DNA segments relative to each other. This system will greatly simplify the current radioactive methods for multiplex genome mapping...
  15. MOLECULAR AND GENETIC ANALYSIS OF THE EEC SYNDROME
    Bernard Weissman; Fiscal Year: 2000
    ..Finally, the determination of the relationship among the various birth defects associated with EEC will allow accurate genetic counseling to individuals with this disorder. ..
  16. PHYSICAL MAP & ORDERED COSMID SET: HUMAN CHROMOSOME 11
    GLEN EVANS; Fiscal Year: 1990
    ..a region including about 1/3 of chromosome 11, and in developing new methods for determining overlaps between the cosmids. The work proposed in this application will apply several different strategies and technologies to the analysis ..
  17. CHEMISTRY OF INHIBIN AND RELATED HORMONES
    DARRELL WARD; Fiscal Year: 1990
    ..The latter type of assay has been very useful in our functional group substitution experiments to learn something of the mechanism of action of thee glycoprotein hormones...
  18. BIOCHEMISTRY AND MOLECULAR GENETICS OF MITOMYCIN BIOSYNT
    David Sherman; Fiscal Year: 2003
    ..Overall, this work will provide an important theoretical and experimental base for future combinatorial biology-based production of novel AHBA-derived natural products using molecular genetic technology. ..
  19. GENE INVOLVED IN SEX REVERSAL MAPS TO CHROMOSOME 9P
    WENDY FLEIJTER; Fiscal Year: 1999
    ..Microsatellite markers will be generated from the new cDNAs isolated to carry out the LOH study. Sequence analysis or SSCP assays on specific genes will be done to look for mutations in the cDNAs isolated in aim 2. ..
  20. CLONING OF PNEUMOCYSTIS CARINII CHROMOSOME ENDS
    Melanie Cushion; Fiscal Year: 2002
    ....
  21. TERATOCARCINOMA AND EMBRYONAL TUMORS--SURFACE ANTIGENS
    Karen Artzt; Fiscal Year: 1992
    ..2) 185 kb of the H-2K region of -haplotypes has been cloned in cosmids and restriction mapped...
  22. ISOLATING THE GENE FOR CHOROIDEREMIA
    Robert Nussbaum; Fiscal Year: 1993
    ..The ultimate goal is to understand what the gene product is and how defects in the gene produce disease...
  23. GENOMIC MAPPING
    KENNETH TARTOF; Fiscal Year: 1991
    ..This process will be repeated with the new set of contiguous clones. Methods for walking with YACs are also proposed and these vectors may be integrated into this scheme...
  24. CLONING HOMOLOGOUS SEGMENTS OF HUMAN XP AND YQ
    PAULINE YEN; Fiscal Year: 1999
    ..In addition, characterization of genes isolated from this region will potentially add to the understanding of the process of spermatogenesis, and improve the management of the clinical problem of male infertility. ..
  25. PATHOGENESIS OF LUNG CANCER AND CHROMOSOME 11
    Gerold Bepler; Fiscal Year: 1999
    ..Genes identified ("candidate" genes) will then be screened for evolutionary conservation, expression in normal lung, and mutations. ..
  26. WILMS TUMOR-1 GENE REGULATION IN KIDNEY DEVELOPMENT
    Vikas Sukhatme; Fiscal Year: 2001
    ..Methods to be used include gel mobility shift assays, DNA footprinting, expression cloning, protein purification, and in situ hybridization. ..
  27. LEISHMANIA GENOME
    Kenneth Stuart; Fiscal Year: 1999
    ....
  28. GIARDIA--A MODEL FOR ANCIENT EUKARYOTIC GENOME ANALYSIS
    Mitchell Sogin; Fiscal Year: 1999
    ..The investigators estimate the total direct cost of the sequencing and mapping proposal (including informatics and phylogenetics) translates into less than $0.20/base. ..
  29. POSITIONAL CLONING OF PYCNODYSOSTOSIS
    Bruce Gelb; Fiscal Year: 2000
    ..by genotyping these families with additional simple tandem repeats (STRs), by developing novel STRs from YACs cosmids, and by finding and analyzing new Pycno families; 2) find genes which localize to the Pycno critical region by ..
  30. STRUCTURAL BASIS OF C3 FUNCTIONS
    BRIAN TACK; Fiscal Year: 1993
    ..The C3 mRNA in deficient and normal family members will be examined by Northern analysis and the defect at the protein level assessed by sequences analysis of potential transcripts of the defective gene...
  31. REPLICATION ASYNCHRONY AND IMPRINTING
    R Hansen; Fiscal Year: 1999
    ..timing and test for gene expression; positive inter-Alu clones will be used for the isolation of cosmid clones; cosmids will be examined for chromosomal location and replication asynchrony by in situ hybridization, for methylation ..
  32. EARLY S PHASE REPLICATION SITES AS ENVIRONMENTAL TARGETS
    David Kaufman; Fiscal Year: 2002
    ....
  33. CLONING OF A LEIOMYOMA ASSOCIATED GENE ON CHROMOSOME 14
    Bradley Quade; Fiscal Year: 1999
    ..has constructed a cosmid library from a leiomyoma cell line containing the t(12;14) translocation and isolated cosmids containing this rearranged region. Under the sponsorship of Dr...
  34. FUNCTION OF CPG-RICH ISLANDS IN HUMAN DNA
    ORLANDO MILLER; Fiscal Year: 1990
    ..The distribution of HpaII and HhaI sites within these cosmids will be determined to see if the rare restriction site clusters fall within HTF islands or are distinct from ..
  35. MOLECULAR STUDIES OF CYSTIC FIBROSIS
    Arthur Beaudet; Fiscal Year: 1990
    ..Overlapping cosmids will be organized into contiguous DNA segments...
  36. CONTROL OF THE GAMMA TO BETA GLOBIN SWITCH
    Deborah Gumucio; Fiscal Year: 2003
    ..This comparative functional analysis of primate globin genes in transgenic mice, combined with studies in extant primates will provide new insights into mechanisms of hemoglobin switching. ..
  37. OSEEN FORCE CYTOMETRY/SORTING
    PAUL STASKUS; Fiscal Year: 1993
    ..Chromosome libraries as larger segments in cosmids (3) and yeast artificial chromosomes (4,5) will require 10 or 100 times as much specific DNA...
  38. PHYSICAL MAP OF HUMAN CHROMOSOME 18
    Uta Francke; Fiscal Year: 1993
    ..to generate a complete physical map of human chromosome 18 and ultimately, a bank of overlapping precisely mapped cosmids and ordered evenly spaced linking clones covering the chromosome from one end to the other...
  39. CHROMOSOME BREAKPOINTS, RENAL & SMALL CELL LUNG CANCER
    David Smith; Fiscal Year: 2000
    ..In the last 8 years of funding on this project we have generated thousands of chromosome 3-specific cosmids, used these cosmids to assist in the cloning of several chromosome 3p translocation breakpoints, generated a ..
  40. ISOLATION OF THE GENE ENCODING THE NA-K-C1 COTRANSPORTER
    J Gargus; Fiscal Year: 1992
    ..This project is a necessary step in the isolation and characterization of the protein(s) responsible for the cotransport mechanism, an important transport mechanism which has thus far eluded structural definition...
  41. REGULATION OF 06-METHYLGUANINE DNA METHYLTRANSFERASE
    Edward Bresnick; Fiscal Year: 1991
    ..The specific aims of this proposal are to: a) isolate the human OMMT gene from 2 cosmids (102 and A-36) that upon transfection are able to confer resistance to chloroethylnitrosourea (CNU) in CHO-D422 (..
  42. CHEMICAL GENETIC & CELLULAR ASPECTS OF IMMUNOGENICITY
    Thomas Gill; Fiscal Year: 1992
    ..A genomic library will be constructed from the R21 strain, and the class I loci will be mapped using overlapping cosmids. The RT1.G locus will be studied by first defining the parental strains and the recombinants by RFLP mapping...
  43. AFFINITY PURIFICATION OF LARGE FRAGMENTS OF HUMAN DNA
    David Ward; Fiscal Year: 1992
    ....
  44. FISSION YEAST GENOME MAPPING
    David Beach; Fiscal Year: 1992
    ..5. Annealing of contigs by hybridization walking. Regions that appear to be unclonable in cosmids will be spanned with yeast YAC vector cloning. 6...
  45. JUVENILE RETINOSCHISIS
    Paul Sieving; Fiscal Year: 2000
    ..2 Mb YAC clone (939h7). A cosmid sublibrary has been made from this clone, and the cosmids are being assembled into a contig...
  46. MOLECULAR DISSECTION OF THE WILLIAMS SYNDROME DELETION
    Uta Francke; Fiscal Year: 1999
    ....
  47. CLONING A GENE AT 12Q22 DELETION IN GERM CELL TUMORS
    Murty Vundavalli; Fiscal Year: 2001
    ..at 12q22 to less than 1 Mb: A small insert genomic contig in the deleted region will be generated by isolating cosmids/PACs that will provide reagents to generate new polymorphic markers...
  48. GENETIC MARKERS, LEUKEMOGENESIS AND THYMIC FUNCTION
    Paul Gottlieb; Fiscal Year: 1992
    ....
  49. DELETION ANALYSIS OF THE SHORT ARM OF CHROMOSOME 5
    JOAN OVERHAUSER; Fiscal Year: 1993
    ..Over 15 breakpoints have been localized to 5pl5.1 alone...
  50. TECHNOLOGIES AIMED AT A PHYSICAL MAP AND SEQUENCE
    JOSEPH SORGE; Fiscal Year: 1990
    ..Let it be clearly stated that Stratagene is undertaking this program as a basic research project and would make all resulting materials widely available for non-commercial research purposes...
  51. ISOLATION & CHARACTERIZATION OF A MAMMALIAN K+ CHANNEL
    J Gargus; Fiscal Year: 1993
    ....
  52. MICROCLONING OF THE DROSOPHILA GENOME
    Vincenzo Pirrotta; Fiscal Year: 1991
    ..The overlaps between cosmids from each microdissected region will be established by restriction mapping...
  53. FIRST INTERNATIONAL WORKSHOP ON CHROMOSOME 18
    MICHELLE LE BEAU; Fiscal Year: 1993
    ..5. To interface with the chromosome 18 committee of the Human Gene Mapping Workshops, and with the CEPH and Eurogem representatives for chromosome 18...
  54. HIGH RESOLUTION GENE MAPPING BY IN SITU HYBRIDIZATION
    David Ward; Fiscal Year: 1999
    ..In order to increase the utility of our map until the complete sequence is obtained, we will use PCR screening and multiplex hybridization to arrayed ESTs to incorporate at least 1000 new markers into the map over the next three years. ..
  55. TUMOR-SPECIFIC ANTIGEN(S) IN HUMAN OVARIAN CARCINOMA
    Chris Platsoucas; Fiscal Year: 1993
    ....
  56. PHYSICAL MAP OF THE C. ELEGANS GENOME
    Robert Waterston; Fiscal Year: 1992
    ..At present the map consists of 700 "contigs" formed by overlapping cosmids, with an average size of 100 kb. Only one hundred of these are associated with genetically positioned sequences...
  57. MOLECULAR BIOLOGY OF TRANSLOCATION ASSOCIATED LEUKEMIAS
    Roberta Adams; Fiscal Year: 1993
    ..The approaches described in this proposal can be applied to the isolation and characterization of any non- random chromosome translocation present in human cancer...
  58. CONSORTIUM TO CONSTRUCT CHROMOSOME 3 FRAMEWORK MAP
    Susan Naylor; Fiscal Year: 1993
    ..Four of the laboratories will isolate highly polymorphic markers from cosmids, YACs, and flow sorted libraries as well as minilibraries for specific regions of the chromosome...
  59. Animal models to design & evaluate improved VZV vaccines
    WAYNE GRAY; Fiscal Year: 2007
    ..The study may also provide support for use of the VZV vaccine as a recombinant vector for immunization against other infectious agents, particularly human immunodeficiency virus (HIV). ..
  60. Postsynthetic Modifications of Bacterial Membrane Lipids
    John Cronan; Fiscal Year: 2006
    ..These processes will be studied by a combination of biochemical, genetic, and molecular biological approaches including tests of pathogenesis in a mouse model system ..
  61. Discovering New Anti-Tuberculosis Drugs
    MICHAEL GEORGE THOMAS; Fiscal Year: 2010
    ..This will be accomplished by combining genetic and molecular engineering of the tuberactinomycin biosynthetic pathways, along with semi-synthetic chemistry. ..
  62. Mechanism of Gamma-Globin Gene Activation in the Adult
    Qiliang Li; Fiscal Year: 2004
    ..Such a development will have important consequences for the treatment of patients with sickle cell disease or beta thalassemia syndromes. ..