viral gene expression regulation

Summary

Summary: Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.

Top Publications

  1. ncbi Papillomaviruses and cancer: from basic studies to clinical application
    Harald zur Hausen
    Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Nat Rev Cancer 2:342-50. 2002
  2. pmc Hepatitis B virus biology
    C Seeger
    Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    Microbiol Mol Biol Rev 64:51-68. 2000
  3. pmc MicroRNAs expressed by herpes simplex virus 1 during latent infection regulate viral mRNAs
    Jennifer Lin Umbach
    Department of Molecular Genetics and Microbiology and Center for Virology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nature 454:780-3. 2008
  4. ncbi Chromatin control of herpes simplex virus lytic and latent infection
    David M Knipe
    Harvard Medical School, Department of Microbiology and Molecular Genetics, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Rev Microbiol 6:211-21. 2008
  5. pmc Kaposi's sarcoma-associated herpesvirus expresses an array of viral microRNAs in latently infected cells
    Xuezhong Cai
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 102:5570-5. 2005
  6. pmc Concurrent expression of latent and a limited number of lytic genes with immune modulation and antiapoptotic function by Kaposi's sarcoma-associated herpesvirus early during infection of primary endothelial and fibroblast cells and subsequent decline of l
    Harinivas H Krishnan
    Department of Microbiology, Molecular Genetics and Immunology, The University of Kansas Medical Center, Kansas City, Kansas, USA
    J Virol 78:3601-20. 2004
  7. ncbi The HIV-1 Rev protein
    V W Pollard
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
    Annu Rev Microbiol 52:491-532. 1998
  8. pmc IL-7 is a potent and proviral strain-specific inducer of latent HIV-1 cellular reservoirs of infected individuals on virally suppressive HAART
    Feng Xiang Wang
    Center for Human Virology and Biodefense, Division of Infectious Diseases and Environmental Medicine, Department of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Clin Invest 115:128-37. 2005
  9. ncbi HIV-1 integration in the human genome favors active genes and local hotspots
    Astrid R W Schroder
    Infectious Disease Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 110:521-9. 2002
  10. pmc Human immunodeficiency virus type 1 can establish latent infection in resting CD4+ T cells in the absence of activating stimuli
    William J Swiggard
    Department of Medicine, University of Pennsylvania, USA
    J Virol 79:14179-88. 2005

Research Grants

  1. Papillomavirus E2 as a cervical/anal cancer drug target
    Janet Brandsma; Fiscal Year: 2005
  2. Interdisciplinary Training in Virology and Gene Therapy
    Ren Sun; Fiscal Year: 2013
  3. Domain structure and interactions of HSV-1 ICP27
    ROZANNE SANDRI GOLDIN; Fiscal Year: 2008
  4. HERPES SIMPLEX VIRUS GENE REGULATION
    ROZANNE SANDRI GOLDIN; Fiscal Year: 2001
  5. MECHANISM OF NON-INTEGRASE-MEDIATED HIV-1 REPLICATION
    Alan Engelman; Fiscal Year: 2001
  6. LEDGF-Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2005
  7. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2009
  8. Helper Phage Mobilization of Staphylococcal Enterotoxin Genes
    GAIL CHRISTIE; Fiscal Year: 2007
  9. MYELOPATHY AND NEUROPATHY DURING CHANGING HIV BURDEN
    Benjamin Gelman; Fiscal Year: 2004
  10. Adult Hodgkin's Disease and Epstein Barr Virus
    Richard Ambinder; Fiscal Year: 2006

Detail Information

Publications286 found, 100 shown here

  1. ncbi Papillomaviruses and cancer: from basic studies to clinical application
    Harald zur Hausen
    Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Nat Rev Cancer 2:342-50. 2002
    ..Epidemiological studies have underlined that HPVs are the main aetiological factor for cervical cancer. But how has this knowledge been translated into the clinic to allow the prevention, screening and treatment of cervical cancer?..
  2. pmc Hepatitis B virus biology
    C Seeger
    Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    Microbiol Mol Biol Rev 64:51-68. 2000
    ..The state of knowledge in this very active field is therefore reviewed with an emphasis on past accomplishments as well as goals for the future...
  3. pmc MicroRNAs expressed by herpes simplex virus 1 during latent infection regulate viral mRNAs
    Jennifer Lin Umbach
    Department of Molecular Genetics and Microbiology and Center for Virology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nature 454:780-3. 2008
    ..Thus, HSV-1 expresses at least two primary miRNA precursors in latently infected neurons that may facilitate the establishment and maintenance of viral latency by post-transcriptionally regulating viral gene expression...
  4. ncbi Chromatin control of herpes simplex virus lytic and latent infection
    David M Knipe
    Harvard Medical School, Department of Microbiology and Molecular Genetics, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Rev Microbiol 6:211-21. 2008
    ....
  5. pmc Kaposi's sarcoma-associated herpesvirus expresses an array of viral microRNAs in latently infected cells
    Xuezhong Cai
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 102:5570-5. 2005
    ..We hypothesize that these viral miRNAs play a critical role in the establishment and/or maintenance of KSHV latent infection in vivo and, hence, in KSHV-induced oncogenesis...
  6. pmc Concurrent expression of latent and a limited number of lytic genes with immune modulation and antiapoptotic function by Kaposi's sarcoma-associated herpesvirus early during infection of primary endothelial and fibroblast cells and subsequent decline of l
    Harinivas H Krishnan
    Department of Microbiology, Molecular Genetics and Immunology, The University of Kansas Medical Center, Kansas City, Kansas, USA
    J Virol 78:3601-20. 2004
    ..These are unique observations among in vitro herpesvirus infections and may have important implications in KSHV biology and pathogenesis...
  7. ncbi The HIV-1 Rev protein
    V W Pollard
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
    Annu Rev Microbiol 52:491-532. 1998
    ..HIV-1 Rev therefore represents an excellent system with which to study aspects of transport across the nuclear envelope...
  8. pmc IL-7 is a potent and proviral strain-specific inducer of latent HIV-1 cellular reservoirs of infected individuals on virally suppressive HAART
    Feng Xiang Wang
    Center for Human Virology and Biodefense, Division of Infectious Diseases and Environmental Medicine, Department of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Clin Invest 115:128-37. 2005
    ....
  9. ncbi HIV-1 integration in the human genome favors active genes and local hotspots
    Astrid R W Schroder
    Infectious Disease Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 110:521-9. 2002
    ..4 kb region containing 1% of sites. These data document unexpectedly strong biases in integration site selection and suggest how selective targeting promotes aggressive HIV replication...
  10. pmc Human immunodeficiency virus type 1 can establish latent infection in resting CD4+ T cells in the absence of activating stimuli
    William J Swiggard
    Department of Medicine, University of Pennsylvania, USA
    J Virol 79:14179-88. 2005
    ..e., are latently infected. Our results show that latent HIV-1 infection occurs in unstimulated resting CD4(+) T cells and suggest a new route for HIV-1 reservoir formation...
  11. ncbi A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA
    P Wei
    Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037 1099, USA
    Cell 92:451-62. 1998
    ..Moreover, overexpression of human cyclin T rescues Tat activity in nonpermissive rodent cells. We propose that Tat directs cyclin T-CDK9 to RNAPII through cooperative binding to TAR RNA...
  12. pmc Herpesviral latency-associated transcript gene promotes assembly of heterochromatin on viral lytic-gene promoters in latent infection
    Qing Yin Wang
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:16055-9. 2005
    ..Thus, HSV, and in particular the HSV LAT gene, may manipulate the cellular histone modification machinery to repress its lytic-gene expression and contribute to the persistence of its genome in a quiescent form in sensory neurons...
  13. pmc Transcription of the herpes simplex virus latency-associated transcript promotes the formation of facultative heterochromatin on lytic promoters
    Anna R Cliffe
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    J Virol 83:8182-90. 2009
    ..Cellular long, noncoding RNAs induce facultative heterochromatin, and this study shows that transcription of a viral noncoding RNA can also induce facultative heterochromatin to promote lytic gene silencing during latency...
  14. pmc Orientation-dependent regulation of integrated HIV-1 expression by host gene transcriptional readthrough
    Yefei Han
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Host Microbe 4:134-46. 2008
    ..Due to the nature of HIV-1 integration sites in vivo, this orientation-dependent regulation can influence the vast majority of infected cells and adds complexity to the maintenance of latency...
  15. ncbi Prostratin antagonizes HIV latency by activating NF-kappaB
    Samuel A Williams
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, San Francisco, California 94141, USA
    J Biol Chem 279:42008-17. 2004
    ..These findings provide new insights into the molecular pathway through which prostratin antagonizes HIV latency highlighting a central role for the action of NF-kappaB...
  16. pmc The polycomb group protein Bmi1 binds to the herpes simplex virus 1 latent genome and maintains repressive histone marks during latency
    Dacia L Kwiatkowski
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, 32610 0266, USA
    J Virol 83:8173-81. 2009
    ..To our knowledge, these are the first data demonstrating that a virus can repress its gene transcription to enter latency by exploiting the mechanism of Polycomb-mediated repression...
  17. pmc Sustained induction of NF-kappa B is required for efficient expression of latent human immunodeficiency virus type 1
    Samuel A Williams
    Gladstone Institute of Virology and Immunology, University of California San Francisco, 1650 Owens Street, San Francisco, CA 94158, USA
    J Virol 81:6043-56. 2007
    ..Conversely, the action of this phosphatase is overcome in the presence of Tat, promoting very efficient RNA Pol II elongation...
  18. ncbi SV40-encoded microRNAs regulate viral gene expression and reduce susceptibility to cytotoxic T cells
    Christopher S Sullivan
    Howard Hughes Medical Institute and Department of Microbiology, G W Hooper Foundation, University of California, San Francisco, California 94143 0552, USA
    Nature 435:682-6. 2005
    ..Thus, viral evolution has taken advantage of the miRNA pathway to generate effectors that enhance the probability of successful infection...
  19. pmc Host and viral proteins in the virion of Kaposi's sarcoma-associated herpesvirus
    Jill T Bechtel
    Department of Microbiology and G W Hooper Foundation, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143 0552, USA
    J Virol 79:4952-64. 2005
    ..With this approach we were able to identify 18 major virion proteins, including structural, regulatory, and signaling proteins of both viral and cellular origin...
  20. ncbi Photosynthesis genes in marine viruses yield proteins during host infection
    Debbie Lindell
    Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 438:86-9. 2005
    ..We thus propose that the phage genes are functional in photosynthesis and that they may be increasing phage fitness by supplementing the host production of these proteins...
  21. pmc Synergistic activation of human immunodeficiency virus type 1 promoter activity by NF-kappaB and inhibitors of deacetylases: potential perspectives for the development of therapeutic strategies
    Vincent Quivy
    Laboratoire de Virologie Moleculaire, Service de Chimie Biologique, Institut de Biologie et de Médecine Moléculaires, Universite Libre de Bruxelles, 6041 Gosselies, Belgium
    J Virol 76:11091-103. 2002
    ..Therefore, our results open new therapeutic strategies aimed at decreasing or eliminating the pool of latently HIV-infected reservoirs by forcing viral expression...
  22. pmc Analysis of human immunodeficiency virus type 1 transcriptional elongation in resting CD4+ T cells in vivo
    Kara G Lassen
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Virol 78:9105-14. 2004
    ....
  23. pmc Viral and cellular microRNAs as determinants of viral pathogenesis and immunity
    Eva Gottwein
    Department of Molecular Genetics and Microbiology and Center for Virology, Duke University Medical Center, Durham, NC 27710, USA
    Cell Host Microbe 3:375-87. 2008
    ..Here, we discuss what is known about how viral and cellular miRNAs influence viral replication and pathogenic potential through their regulation of viral mRNAs or by reshaping cellular gene expression...
  24. ncbi The 7SK small nuclear RNA inhibits the CDK9/cyclin T1 kinase to control transcription
    Z Yang
    Department of Molecular and Cell Biology, University of California at Berkeley, USA
    Nature 414:317-22. 2001
    ..Our studies demonstrate the involvement of an snRNA in controlling the activity of a Cdk-cyclin kinase...
  25. pmc The lytic switch protein of KSHV activates gene expression via functional interaction with RBP-Jkappa (CSL), the target of the Notch signaling pathway
    Yuying Liang
    Howard Hughes Medical Institute and Departments of Microbiology and Medicine, University of California, San Francisco, California 94143 0414, USA
    Genes Dev 16:1977-89. 2002
    ..These results indicate that KSHV has evolved a ligand-independent mechanism for constitutive activation of the Notch pathway as a part of its strategy for reactivation from latency...
  26. ncbi Virus infection induces the assembly of coordinately activated transcription factors on the IFN-beta enhancer in vivo
    M G Wathelet
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Mol Cell 1:507-18. 1998
    ..These observations provide a unique example of the coordinate activation of multiple transcriptional activator proteins and their highly cooperative assembly into a transcriptional enhancer complex in vivo...
  27. pmc Specific histone tail modification and not DNA methylation is a determinant of herpes simplex virus type 1 latent gene expression
    Nicole J Kubat
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida 32610 0266, USA
    J Virol 78:1139-49. 2004
    ..These data suggest that histone composition may be a major regulatory determinant of HSV latency...
  28. pmc Chromatin remodeling of the Kaposi's sarcoma-associated herpesvirus ORF50 promoter correlates with reactivation from latency
    Fang Lu
    The Wistar Institute Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Virol 77:11425-35. 2003
    ..Together, these results suggest that remodeling of a stably positioned nucleosome at the transcriptional initiation site of ORF50 is a regulatory step in the transition from latent to lytic infection...
  29. pmc The vaccinia virus-stimulated mitogen-activated protein kinase (MAPK) pathway is required for virus multiplication
    Anderson A Andrade
    Grupo de Transdução de Sinal, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Av Antonio Carlos, 6627, Campus Pampulha, 31270 901, Belo Horizonte, Minas Gerais, Brazil
    Biochem J 381:437-46. 2004
    ..e. post-penetration, but is also crucial to define the fate of virus progeny...
  30. pmc TRBP, a regulator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing
    Astrid D Haase
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4508 Basel, Switzerland
    EMBO Rep 6:961-7. 2005
    ..The TRBP-Dicer interaction shown raises interesting questions about the potential interplay between RNAi and interferon-PKR pathways...
  31. ncbi Reactivation of Epstein-Barr virus: regulation and function of the BZLF1 gene
    S H Speck
    Dept of Pathology, Washington University School of Medicine, St Louis, MO 63110, USA
    Trends Microbiol 5:399-405. 1997
    ..Understanding this switch may facilitate development of strategies to interfere with EBV infection...
  32. ncbi Development of a Sindbis virus expression system that efficiently expresses green fluorescent protein in midguts of Aedes aegypti following per os infection
    K E Olson
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Colorado State University, Ft Collins, CO 80523, USA
    Insect Mol Biol 9:57-65. 2000
    ..The MRE3'2 J dsSIN expression system should be an important tool for efficient gene expression in Ae. aegypti midguts...
  33. pmc Methylation of Tat by PRMT6 regulates human immunodeficiency virus type 1 gene expression
    Marie Chloe Boulanger
    Terry Fox Molecular Oncology Group, Lady Davis Institute, Sir Mortimer B Davis Jewish General Hospital, Montreal, Quebec, Canada
    J Virol 79:124-31. 2005
    ..Our study demonstrates that arginine methylation of Tat negatively regulates its transactivation activity and that PRMT6 acts as a restriction factor for HIV replication...
  34. ncbi HIV-1 Tat reprograms immature dendritic cells to express chemoattractants for activated T cells and macrophages
    Elena Izmailova
    Department of Medicine, Children s Hospital, and Harvard Medical School, Boston, Massachusetts, USA
    Nat Med 9:191-7. 2003
    ..These results show that HIV-1 Tat reprograms host dendritic cell gene expression to facilitate expansion of HIV-1 infection...
  35. ncbi Tat stimulates cotranscriptional capping of HIV mRNA
    Ya Lin Chiu
    Chemical Biology Program, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Cell 10:585-97. 2002
    ..Our findings implicate capping in an elongation checkpoint critical to HIV gene expression...
  36. pmc Coronaviruses as vectors: position dependence of foreign gene expression
    Cornelis A M de Haan
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine and Institute of Biomembranes, Utrecht University, 3584 CL Utrecht, The Netherlands
    J Virol 77:11312-23. 2003
    ..Altogether, our observations clearly demonstrate the potential of coronaviruses as (multivalent) expression vectors...
  37. pmc Viral regulation of mRNA export
    Rozanne M Sandri-Goldin
    Department of Microbiology and Molecular Genetics, University of California, Irvine, California 92697 4025, USA
    J Virol 78:4389-96. 2004
  38. ncbi Hexamethylbisacetamide and disruption of human immunodeficiency virus type 1 latency in CD4(+) T cells
    Shailesh K Choudhary
    Departments of Medicine, University of North Carolina at Chapel Hill 27599 7435, USA
    J Infect Dis 197:1162-70. 2008
    ....
  39. pmc Trimethylation of histone H3 lysine 4 by Set1 in the lytic infection of human herpes simplex virus 1
    Jing Huang
    The Wistar Institute, Philadelphia, PA 19104, USA
    J Virol 80:5740-6. 2006
    ....
  40. pmc Laser-capture microdissection: refining estimates of the quantity and distribution of latent herpes simplex virus 1 and varicella-zoster virus DNA in human trigeminal Ganglia at the single-cell level
    Kening Wang
    Medical Virology Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA
    J Virol 79:14079-87. 2005
    ..These data indicate that both HSV-1 and VZV DNAs persist in human TG primarily, if not exclusively, in a moderate percentage of neuronal cells...
  41. ncbi The KSHV immediate-early transcription factor RTA encodes ubiquitin E3 ligase activity that targets IRF7 for proteosome-mediated degradation
    Yanxing Yu
    Molecular Virology Laboratories, Viral Oncology Program, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, 1650 Orleans Street, Baltimore, MD 2123, USA
    Immunity 22:59-70. 2005
    ..Therefore, manipulation of the stability and function of IRF7 by the KSHV RTA transcription factor provides an unexpected regulatory strategy for circumventing the innate immune defence system...
  42. pmc Translational control of viral gene expression in eukaryotes
    M Gale
    University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Microbiol Mol Biol Rev 64:239-80. 2000
    ..We discuss how these translational strategies have been incorporated into the virus life cycle and examine how such programming contributes to the pathogenesis of the host cell...
  43. ncbi Virus-induced heterodimer formation between IRF-5 and IRF-7 modulates assembly of the IFNA enhanceosome in vivo and transcriptional activity of IFNA genes
    Betsy J Barnes
    Sidney Kimmel Comprehensive Cancer Center, Baltimore, Maryland 21231, USA
    J Biol Chem 278:16630-41. 2003
    ....
  44. ncbi The regulation of HIV-1 gene expression: the emerging role of chromatin
    Guocheng He
    University of Texas Southwestern Medical Center at Dallas, Department of Medicine, Division of Infectious Diseases, Dallas, Texas 75390 9113, USA
    DNA Cell Biol 21:697-705. 2002
    ..A better understanding of the role of chromatin in the regulation of HIV expression could lead to much-needed therapies against proviral genomes that are being actively transcribed, and those that are quiescent and persistent...
  45. pmc NF-kappaB inhibits gammaherpesvirus lytic replication
    Helen J Brown
    Department of Molecular and Medical Pharmacology, University of California at Los Angeles, Los Angeles, California 90095, USA
    J Virol 77:8532-40. 2003
    ..They also suggest that NF-kappaB may be a novel target for the disruption of virus latency and therefore the treatment of gammaherpesvirus-related malignancies...
  46. pmc Long-distance RNA-RNA interactions in plant virus gene expression and replication
    W Allen Miller
    Plant Pathology Department, Iowa State University, Ames, Iowa 50011, USA
    Annu Rev Phytopathol 44:447-67. 2006
    ..We emphasize viruses in the Tombusviridae and Luteoviridae families in which long-distance interactions are best characterized, but similar phenomena in other viruses are also discussed. Many more examples likely remain undiscovered...
  47. pmc Control of stochastic gene expression by host factors at the HIV promoter
    John C Burnett
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, California, United States of America
    PLoS Pathog 5:e1000260. 2009
    ..These results may have biomedical implications for the treatment of HIV latency...
  48. pmc Human cellular microRNA hsa-miR-29a interferes with viral nef protein expression and HIV-1 replication
    Jasmine K Ahluwalia
    Institute of Genomics and Integrative Biology IGIB CSIR, Delhi, India
    Retrovirology 5:117. 2008
    ..Computational predictions revealed earlier that the HIV-1 genome includes regions that may be potentially targeted by human miRNAs. Here we report the functionality of predicted miR-29a target site in the HIV-1 nef gene...
  49. ncbi Methylation of SPT5 regulates its interaction with RNA polymerase II and transcriptional elongation properties
    Youn Tae Kwak
    Division of Hematology Oncology, Department of Medicine, University of Texas Southwestern Medical Center, Dallas 75390, USA
    Mol Cell 11:1055-66. 2003
    ..These results suggest that methylation of SPT5 is an important posttranslational modification that is involved in regulating its transcriptional elongation properties in response to viral and cellular factors...
  50. ncbi The latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus modulates cellular gene expression and protects lymphoid cells from p16 INK4A-induced cell cycle arrest
    Feng Qi An
    Division of Hematology Oncology and the Department of Molecular Biology and Microbiology, Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 280:3862-74. 2005
    ..Under these conditions, LANA expression protects lymphoid cells from p16 INK4a-induced cell cycle arrest and induces S-phase entry...
  51. ncbi HIV Tat, its TARgets and the control of viral gene expression
    Claudio Brigati
    Molecular Oncology Laboratory, National Cancer Research Institute, c o Centro di Biotecnologie Avanzante, Largo Rosanna Benzi 10, 16132 Genova, Italy
    FEMS Microbiol Lett 220:57-65. 2003
    ..Finally, extracellular Tat can be internalized by cells through an active endocytosis process. Here we discuss some of the molecular mechanisms involved in intracellular and extracellular Tat function...
  52. pmc Functional roles for the TATA promoter and enhancers in basal and Tat-induced expression of the human immunodeficiency virus type 1 long terminal repeat
    B Berkhout
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892
    J Virol 66:139-49. 1992
    ..Our results do not allow conclusions about whether Tat acts at the level of initiation or at the level of elongation to be drawn...
  53. pmc HIV reproducibly establishes a latent infection after acute infection of T cells in vitro
    Albert Jordan
    Department of Medicine, University of California, San Francisco, CA 94141, USA
    EMBO J 22:1868-77. 2003
    ..These observations demonstrate that HIV can reproducibly establish a latent infection as a consequence of integration in or near heterochromatin...
  54. ncbi Role of viral splicing elements and cellular RNA binding proteins in regulation of HIV-1 alternative RNA splicing
    C Martin Stoltzfus
    Department of Microbiology, University of Iowa, Iowa City, 52242, USA
    Curr HIV Res 4:43-55. 2006
    ..Finally we will review evidence suggesting the possibility that there may be changes in regulation of HIV-1 alternative splicing in infected human T cells, human macrophages and rodent cells...
  55. pmc The cellular RNA export receptor TAP/NXF1 is required for ICP27-mediated export of herpes simplex virus 1 RNA, but the TREX complex adaptor protein Aly/REF appears to be dispensable
    Lisa A Johnson
    Department of Microbiology and Molecular Genetics, School of Medicine, Medical Sciences I, Rm B240, University of California at Irvine, Irvine, CA 92697 4025, USA
    J Virol 83:6335-46. 2009
    ..In contrast, a decrease in TAP/NXF1 levels severely impaired export of ICP27 and poly(A)(+) RNA. We conclude that TAP/NXF1 is essential for ICP27-mediated export of RNA during HSV-1 infection, whereas Aly/REF may be dispensable...
  56. pmc Human herpesvirus 8 LANA interacts with proteins of the mSin3 corepressor complex and negatively regulates Epstein-Barr virus gene expression in dually infected PEL cells
    A Krithivas
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland 21231, USA
    J Virol 74:9637-45. 2000
    ....
  57. pmc The p23 protein of citrus tristeza virus controls asymmetrical RNA accumulation
    Tatineni Satyanarayana
    Citrus Research and Education Center, University of Florida, Lake Alfred, Florida 33850, USA
    J Virol 76:473-83. 2002
    ..Thus, the p23 protein controls asymmetric accumulation of CTV RNAs by downregulating negative-stranded RNA accumulation and indirectly increases expression of 3' genes...
  58. pmc Control of cytomegalovirus lytic gene expression by histone acetylation
    Jane C Murphy
    Department of Medicine, University of Cambridge, Cambridge CB2 2QQ, UK
    EMBO J 21:1112-20. 2002
    ....
  59. pmc Open reading frame 50 protein of Kaposi's sarcoma-associated herpesvirus directly activates the viral PAN and K12 genes by binding to related response elements
    Pey Jium Chang
    Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut, USA
    J Virol 76:3168-78. 2002
    ..Two KSHV lytic-cycle promoters are activated by a similar mechanism that involves direct recognition of a homologous response element by the DNA binding domain of ORF 50 protein in the context of related cellular proteins...
  60. pmc Transcriptional competence of the integrated HIV-1 provirus at the nuclear periphery
    Mariacarolina Dieudonne
    Laboratory of Molecular Virology, International Centre for Genetic Engineering and Biotechnology ICGEB, Trieste, Italy
    EMBO J 28:2231-43. 2009
    ....
  61. pmc Transcriptional regulation of the interleukin-6 gene of human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus)
    Hongyu Deng
    Department of Molecular and Medical Pharmacology, UCLA AIDS Institute, Jonsson Comprehensive Cancer Center, University of California at Los Angeles, 90095, USA
    J Virol 76:8252-64. 2002
    ..We propose that the existence of two vIL-6 promoters offers opportunities for differential regulation of vIL-6 gene expression in different tissue types and may account for the variable vIL-6 levels observed in KS, PEL, and MCD...
  62. pmc Transcription activation of polyadenylated nuclear rna by rta in human herpesvirus 8/Kaposi's sarcoma-associated herpesvirus
    M J Song
    Department of Molecular and Medical Pharmacology, UCLA AIDS Institute, Jonnson Comprehensive Cancer Center, and Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095, USA
    J Virol 75:3129-40. 2001
    ..This study has highlighted an important mechanism controlling PAN RNA expression and also provides a model system for investigating how Rta transactivates gene expression during lytic replication...
  63. ncbi Molecular biology and pathogenesis of Kaposi sarcoma-associated herpesvirus
    Subhash C Verma
    Department of Microbiology and Tumor Virology Program of the Abramson Comprehensive Cancer Center, University of Pennsylvania School of Medicine, 201E Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104, USA
    FEMS Microbiol Lett 222:155-63. 2003
    ..LANA has now been shown to be a multifunctional protein involved in numerous cellular functions including binding to the retinoblastoma protein and p53, regulating cell proliferation and apoptosis...
  64. pmc Ikappabeta-related vankyrin genes in the Campoletis sonorensis ichnovirus: temporal and tissue-specific patterns of expression in parasitized Heliothis virescens lepidopteran hosts
    Jeremy A Kroemer
    University of Kentucky, Department of Entomology, S 225 Agricultural Sciences Center North, Lexington, KY 40546, USA
    J Virol 79:7617-28. 2005
    ..We hypothesize that PDV vankyrin genes may suppress NF-kappabeta activity during immune responses and developmental cascades in parasitized lepidopteran hosts of C. sonorensis...
  65. pmc Potential role for luman, the cellular homologue of herpes simplex virus VP16 (alpha gene trans-inducing factor), in herpesvirus latency
    R Lu
    Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4, Canada
    J Virol 74:934-43. 2000
    ..Interestingly, Luman could activate the promoters of IE110 and LAT, two genes that are critical for reactivation of HSV-1 from latency. This suggests a role for Luman in the reactivation process as well...
  66. pmc Acetylation of the latency-associated nuclear antigen regulates repression of Kaposi's sarcoma-associated herpesvirus lytic transcription
    Fang Lu
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    J Virol 80:5273-82. 2006
    ..These findings support the role of LANA as a transcriptional repressor of lytic reactivation and provide evidence that lysine acetylation regulates LANA interactions with chromatin, Sp1, and ORF50 promoter DNA...
  67. pmc EC5S ubiquitin complex is recruited by KSHV latent antigen LANA for degradation of the VHL and p53 tumor suppressors
    Qi Liang Cai
    Department of Microbiology and the Tumor Virology Program, Abramson Comprehensive Cancer Center, University of Pennsylvania Medical School, Philadelphia, Pennsylvania, USA
    PLoS Pathog 2:e116. 2006
    ..Thus, manipulation of tumor suppressors by LANA potentially provides a favorable environment for progression of KSHV-infected tumor cells...
  68. pmc PRMT6 diminishes HIV-1 Rev binding to and export of viral RNA
    Cédric F Invernizzi
    McGill University AIDS Centre, Lady Davis Institute for Medical Research, Sir Mortimer B, Davis Jewish General Hospital, Montreal, Quebec H3T 1E2, Canada
    Retrovirology 3:93. 2006
    ..Since Tat is known to be asymmetrically arginine dimethylated by protein arginine methyltransferase 6 (PRMT6) in its arginine rich motif, we investigated whether the Rev protein could act as a substrate for this enzyme...
  69. pmc Different members of the Sp1 multigene family exert opposite transcriptional regulation of the long terminal repeat of HIV-1
    B Majello
    Dipartimento di Genetica, Biologia Generale e Molecolare, Universita Federico II, Naples, Italy
    Nucleic Acids Res 22:4914-21. 1994
    ..We found that the cooperative interaction between NF-kappa B and Sp1 is highly specific, since neither Sp3 nor Sp4 is capable of cooperating with NF-kappa B...
  70. ncbi Unravelling hepatitis C virus replication from genome to function
    Brett D Lindenbach
    Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Nature 436:933-8. 2005
    ..Nevertheless hard-fought progress has been made and the first wave of antiviral drugs is entering clinical trials...
  71. pmc HIV-1 Vpr-mediated G2 arrest involves the DDB1-CUL4AVPRBP E3 ubiquitin ligase
    Jean Philippe Belzile
    Laboratory of Human Retrovirology, Institut de Recherches Cliniques de Montreal, Montreal, Quebec, Canada
    PLoS Pathog 3:e85. 2007
    ....
  72. ncbi Rev-independent expression of synthetic gag-pol genes of human immunodeficiency virus type 1 and simian immunodeficiency virus: implications for the safety of lentiviral vectors
    R Wagner
    Institut für medizinische Mikrobiologie and Hygiene, Universitat Regensburg, D 93053 Regensburg, Germany
    Hum Gene Ther 11:2403-13. 2000
    ..By eliminating regions of homology and sequences involved in packaging, synthetic gag-pol genes should improve the safety profile of lentiviral vectors...
  73. ncbi Regulatory elements of hepatitis B virus transcription
    Naazneen Moolla
    Hepatitis B Virus Research Programme, Department of Molecular Medicine and Haematology, University of the Witwatersrand Medical School, 7 York Road, Parktown 2193, South Africa
    J Viral Hepat 9:323-31. 2002
    ....
  74. pmc Human cytidine deaminase APOBEC3H restricts HIV-1 replication
    Ying Dang
    Department of Microbiology and Molecular Genetics, College of Osteopathic Medicine, Michigan State University, East Lansing, Michigan 48824 4320, USA
    J Biol Chem 283:11606-14. 2008
    ..Thus, A3H inhibits HIV-1 replication potently by a cytidine deamination-independent mechanism, and optimizing A3H expression in vivo should represent a novel therapeutic strategy for HIV-1 treatment...
  75. pmc The complementary strand of the human T-cell leukemia virus type 1 RNA genome encodes a bZIP transcription factor that down-regulates viral transcription
    Gilles Gaudray
    Laboratoire Infections Retrovirales et Signalisation Cellulaire, CNRS UM I UMR 5121, Institut de Biologie, 4 Boulevard Henri IV, 34060 Montpellier, France
    J Virol 76:12813-22. 2002
    ..Moreover, the characterization of a minus-strand gene protein encoded by HTLV-1 has never been reported until now...
  76. pmc Nuclear retention of multiply spliced HIV-1 RNA in resting CD4+ T cells
    Kara G Lassen
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    PLoS Pathog 2:e68. 2006
    ..Whether through effects on RNA export or another mechanism, the ability of PTB to reverse latency without inducing cellular activation is a result with therapeutic implications...
  77. ncbi Regulation of human endogenous retrovirus W protein expression by herpes simplex virus type 1: implications for multiple sclerosis
    Klemens Ruprecht
    Department of Neurology, Clinical Research Unit for Multiple Sclerosis and Neuroimmunology, Wurzburg, Germany
    J Neurovirol 12:65-71. 2006
    ....
  78. pmc Lytic but not latent infection by Kaposi's sarcoma-associated herpesvirus requires host CSL protein, the mediator of Notch signaling
    Yuying Liang
    Howard Hughes Medical Institute and Department of Microbiology, University of California Medical Center, San Francisco, CA 94143 0414, USA
    Proc Natl Acad Sci U S A 100:8490-5. 2003
    ..RBP-J kappa-mediated repression is therefore not essential for establishment of latent infection, but the RTA-mediated redirection of RBP-J kappa activity from repression to activation is critical for lytic viral replication...
  79. pmc A bidirectional SF2/ASF- and SRp40-dependent splicing enhancer regulates human immunodeficiency virus type 1 rev, env, vpu, and nef gene expression
    Massimo Caputi
    Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA
    J Virol 78:6517-26. 2004
    ..4, even when the 5' splice site and the U1 snRNA have been mutated to obtain a perfect complementary match. The ESE characterized here is highly conserved in most viral subtypes...
  80. pmc Detection, characterization and regulation of antisense transcripts in HIV-1
    Sebastien Landry
    Universite du Quebec a Montreal, Departement des Sciences Biologiques, Montreal Quebec, H2X 3X8, Canada
    Retrovirology 4:71. 2007
    ..The objective of the present study was to demonstrate the existence of antisense transcription in HIV-1 and to provide a better characterization of the transcript and its regulation...
  81. ncbi Transient-mediated fate determination in a transcriptional circuit of HIV
    Leor S Weinberger
    Department of Chemistry and Biochemistry, University of California San Diego, 9500 Gilman Drive 0314, La Jolla, California 92093, USA
    Nat Genet 40:466-70. 2008
    ..Thus, transcriptional positive feedback can modulate transient expression lifetime to a greater extent than protein half-life modulation, and it has a critical role in the cell-fate decision in HIV...
  82. pmc Translation of the first upstream ORF in the hepatitis B virus pregenomic RNA modulates translation at the core and polymerase initiation codons
    Augustine Chen
    Biochemistry Department, University of Otago Dunedin, New Zealand
    Nucleic Acids Res 33:1169-81. 2005
    ..Our results indicate that the C0 ORF is a determinant in balancing the synthesis of the core and polymerase proteins...
  83. ncbi Immune control of herpes simplex virus during latency
    Kamal M Khanna
    Graduate Program in Immunology, Department of Ophthalmology, University of Pittsburgh, 203 Lothrop Street, Pittsburgh, PA 15213 2588, USA
    Curr Opin Immunol 16:463-9. 2004
    ....
  84. ncbi HIV-1 Vpu sequesters beta-transducin repeat-containing protein (betaTrCP) in the cytoplasm and provokes the accumulation of beta-catenin and other SCFbetaTrCP substrates
    Corinne Besnard-Guerin
    Institut Cochin, Department of Infectious Diseases, INSERM U567, CNRS UMR 8104, Université R Descartes Paris V, 27 rue du Faubourg St Jacques, 75014 Paris, France
    J Biol Chem 279:788-95. 2004
    ..These data suggest that Vpu is a strong competitive inhibitor of betaTrCP that impairs the degradation of SCFbetaTrCP substrates as long as Vpu has an intact phosphorylation motif and can bind to betaTrCP...
  85. pmc Wide-scale use of Notch signaling factor CSL/RBP-Jkappa in RTA-mediated activation of Kaposi's sarcoma-associated herpesvirus lytic genes
    Linda M Persson
    Department of Microbiology, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    J Virol 84:1334-47. 2010
    ..These findings broaden the role for CSL in coordinating the KSHV lytic gene expression program and help to define a signature motif for functional CSL sites within the viral genome...
  86. pmc Herpes simplex virus type 1 promoter activity during latency establishment, maintenance, and reactivation in primary dorsal root neurons in vitro
    J L Arthur
    Division of Virology, Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    J Virol 75:3885-95. 2001
    ....
  87. ncbi A sequence required for -1 ribosomal frameshifting located four kilobases downstream of the frameshift site
    C P Paul
    Plant Pathology Department, Iowa State University, Ames 50011, USA
    J Mol Biol 310:987-99. 2001
    ..Most importantly, the remarkably long-distance interaction reported here suggests the presence of a novel structure that can facilitate ribosomal frameshifting...
  88. pmc Murine gammaherpesvirus 68 open reading frame 24 is required for late gene expression after DNA replication
    Elaine Wong
    Department of Molecular and Medical Pharmacology, Dental Research Institute, David Geffen School of Medicine, University of California, Los Angeles, 23 120 Center for Health Sciences, Los Angeles, CA 90095 1735, USA
    J Virol 81:6761-4. 2007
    ..Thus, we have identified an MHV-68 protein, ORF24, that is essential for the expression of viral late proteins yet dispensable for viral DNA replication...
  89. pmc A splicing enhancer in the E4 coding region of human papillomavirus type 16 is required for early mRNA splicing and polyadenylation as well as inhibition of premature late gene expression
    Margaret Rush
    Department of Medical Biochemistry and Microbiology, Uppsala University, Biomedical Centre, Husargatan 3, Box 582, 75123 Uppsala, Sweden
    J Virol 79:12002-15. 2005
    ....
  90. pmc Epstein-Barr virus-encoded microRNA miR-BART2 down-regulates the viral DNA polymerase BALF5
    Stephanie Barth
    Institute of Virology, University of Saarland Medical School, 66424 Homburg, Germany
    Nucleic Acids Res 36:666-75. 2008
    ..Our results are compatible with the notion that EBV-miR-BART2 inhibits transition from latent to lytic viral replication...
  91. ncbi Intratumor injection of small interfering RNA-targeting human papillomavirus 18 E6 and E7 successfully inhibits the growth of cervical cancer
    Takuma Fujii
    Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo 160 8582, Japan
    Int J Oncol 29:541-8. 2006
    ..These results indicate that atelocollagen-mediated delivery of siRNA HPV18 E6 and E7 can be used as a novel therapeutic approach for cervical cancer...
  92. ncbi Synergistic stimulation of HIV-1 rev-dependent export of unspliced mRNA to the cytoplasm by hnRNP A1
    I Najera
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    J Mol Biol 285:1951-64. 1999
    ..We conclude that hnRNP A1 is able to direct unspliced globin pre-mRNA into a nuclear compartment where it is recognised by Rev and then transported to the cytoplasm...
  93. pmc Virus maturation by budding
    H Garoff
    Department of Biosciences at Novum, S 141 57 Huddinge, Sweden
    Microbiol Mol Biol Rev 62:1171-90. 1998
    ..This new concept also provides answers to the question of how viral and cellular membrane proteins are sorted during budding. In addition, it has implications for the mechanism by which the virion is uncoated during virus entry...
  94. pmc Autorepression of the human cytomegalovirus major immediate-early promoter/enhancer at late times of infection is mediated by the recruitment of chromatin remodeling enzymes by IE86
    Matthew Reeves
    Department of Medicine, Box 157, Level 5, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2QQ, United Kingdom
    J Virol 80:9998-10009. 2006
    ....
  95. pmc The human immunodeficiency virus type 1 promoter contains a CATA box instead of a TATA box for optimal transcription and replication
    Tim van Opijnen
    Department of Human Retrovirology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
    J Virol 78:6883-90. 2004
    ..However, this replication rate may be optimal in the in vivo context of an infected individual...
  96. pmc Comparative study of regulation of RTA-responsive genes in Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8
    Moon Jung Song
    Department of Molecular and Medical Pharmacology, UCLA AIDS Institute, Jonsson Comprehensive Cancer Center, and Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095, USA
    J Virol 77:9451-62. 2003
    ..Based on these results, we conclude that direct binding of RTA to these target sequences contributes to their gene expression to various extents during the lytic life cycle of KSHV...
  97. pmc Suppression of immediate-early viral gene expression by herpesvirus-coded microRNAs: implications for latency
    Eain Murphy
    Simons Center for Systems Biology, Institute for Advanced Study, Princeton, NJ 08540, USA
    Proc Natl Acad Sci U S A 105:5453-8. 2008
    ..We propose that herpesviruses use microRNA-mediated suppression of immediate-early genes as part of their strategy to enter and maintain latency...
  98. pmc A SAP30 complex inhibits IFN-beta expression in Rift Valley fever virus infected cells
    Nicolas Le May
    Unite de Genetique Moleculaire des Bunyavirus, Institut Pasteur, Paris, France
    PLoS Pathog 4:e13. 2008
    ..We discuss here the strategy developed by the highly pathogenic RVFV to evade the host antiviral response, affecting nuclear organization and IFN-beta promoter chromatin structure...
  99. pmc The transcriptional cycle of HIV-1 in real-time and live cells
    Stephanie Boireau
    Institute of Molecular Genetics of Montpellier, Unite Mixte de Recherche 5535, Centre National de la Recherche Scientifique, 34293 Montpellier, France
    J Cell Biol 179:291-304. 2007
    ..The strengths and limitations of this kinetic approach to analyze mRNA biogenesis in living cells are discussed...
  100. pmc Activation of Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) lytic replication by human cytomegalovirus
    J Vieira
    Department of Laboratory Medicine, University of Washington, and Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    J Virol 75:1378-86. 2001
    ..These data demonstrate that HCMV can activate KSHV lytic replication and suggest that HCMV could influence KSHV pathogenesis...
  101. pmc Cyclin/CDK regulates the nucleocytoplasmic localization of the human papillomavirus E1 DNA helicase
    Wentao Deng
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, 510 McCallum Basic Health Sciences Building, 1918 University Blvd, Birmingham, AL 35294 0005, USA
    J Virol 78:13954-65. 2004
    ..Collectively, our data demonstrate that CDK phosphorylation controls E1 nuclear localization to support viral DNA amplification. Thus, HPV adopts and adapts the cellular regulatory mechanism to complete its reproductive program...

Research Grants71

  1. Papillomavirus E2 as a cervical/anal cancer drug target
    Janet Brandsma; Fiscal Year: 2005
    ..Vectors containing a conditional wild-type CRPV E2 transgene will be used to determine the effects of E2 overexpression. ..
  2. Interdisciplinary Training in Virology and Gene Therapy
    Ren Sun; Fiscal Year: 2013
    ..The research interests of our faculty encompass viral entry, viral gene expression regulation, replication of viral genome, cell biology during viral infection, viral particle assembly, viral ..
  3. Domain structure and interactions of HSV-1 ICP27
    ROZANNE SANDRI GOLDIN; Fiscal Year: 2008
    ..abstract_text> ..
  4. HERPES SIMPLEX VIRUS GENE REGULATION
    ROZANNE SANDRI GOLDIN; Fiscal Year: 2001
    ....
  5. MECHANISM OF NON-INTEGRASE-MEDIATED HIV-1 REPLICATION
    Alan Engelman; Fiscal Year: 2001
    ..Further aims are to develop gene therapy vectors based on non-integrating retroviruses. ..
  6. LEDGF-Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2005
    ..abstract_text> ..
  7. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2009
    ..abstract_text> ..
  8. Helper Phage Mobilization of Staphylococcal Enterotoxin Genes
    GAIL CHRISTIE; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  9. MYELOPATHY AND NEUROPATHY DURING CHANGING HIV BURDEN
    Benjamin Gelman; Fiscal Year: 2004
    ..Together, the studies we have proposed will provide insights concerning prevalent AIDS morbidities in the HAART era. ..
  10. Adult Hodgkin's Disease and Epstein Barr Virus
    Richard Ambinder; Fiscal Year: 2006
    ..This work should lay the groundwork for future viral antigen targeted therapies. ..
  11. Molecular Mechanisms in Reovirus mRNA Synthesis
    MAX NIBERT; Fiscal Year: 2006
    ..The results of these studies will enhance our understanding of the reovirus core as an elegantly designed molecular machine for mRNA synthesis. ..
  12. Nicotine in HIV-1 and Mycobacterium tuberculosis Neuropathogenesis
    ROBERT ROCK; Fiscal Year: 2008
    ..abstract_text> ..
  13. EUKARYOTIC GENE REGULATION AS STUDIED IN YEAST
    Mark Ptashne; Fiscal Year: 2009
    ..In particular we are testing the idea that this protein, a component of the large complex called the Mediator, is rapidly degraded in cells, and that this instability facilitates activation of transcription ..
  14. REGULATION OF HIV-1 RNA SPLICING
    CONRAD STOLTZFUS; Fiscal Year: 2010
    ..Group O viruses appear to use different cis elements than Group M HIV-1 strains to regulate splicing. Identification of these elements may give us new insights about the evolution of HIV-1. ..
  15. Molecular basis of neuronal dysfunction in AIDS
    Francesca Peruzzi; Fiscal Year: 2008
    ..The outcome of these studies will provide a new information on the pathways which are affected by HIV-1 Tat, and will assist to improve our effort for developing strategies against HIV-1 induced neuronal cell injury. ..
  16. VIRUS SPECIFIC FACTORS OF HSV TO CORNEAL DISEASE
    CURTIS BRANDT; Fiscal Year: 2008
    ..g. isoform A is involved with function B). These studies will add to our knowledge of the role of the US1 protein in ocular HSV infections and may lead to improved therapies. ..
  17. Functions of EBNA1 in replication & partitioning of EBV
    Ashok Aiyar; Fiscal Year: 2009
    ..2. To utilize a novel replicon and fusion protein to understand the function of EBNA1 in replication. 3. To use viral genetics to determine the functions of EBNA1 in the immortalization of naive B-cells by EBV ..
  18. EBV GENOME EXPRESSION:LOCALIZATION OF SPECIFIC FUNCTIONS
    S Hayward; Fiscal Year: 2009
    ..Aim 3. To develop a mouse model for in vivo evaluation of EBV promoter expression using hematopoietic reconstitution with lentiviral transduced stem cells. ..
  19. Maturation functions of the HSV-1 tegument
    Prashant Desai; Fiscal Year: 2010
    ..translocation, Golgi stabilization, Golgi trafficking, virion incorporation and protein-protein interactions This analysis will identify domains of the proteins that have the potential to be used as targets for antiviral intervention ..
  20. Immunobiology of Lung Fibrosis
    Ana Mora; Fiscal Year: 2007
    ..4. Using IL-4 receptor deficient mice with and without adoptive transfer of normal Th2 cells, to determine whether IL-4 signaling in cells other than T lymphocytes is important to the lung fibrogenic response to MHV68 or bleomycin. ..
  21. Promoters for Glaucoma Gene Therapy
    CURTIS BRANDT; Fiscal Year: 2007
    ..abstract_text> ..
  22. Cis-acting RNA elements in hepatitis C virus replication
    Guangxiang Luo; Fiscal Year: 2007
    ..abstract_text> ..
  23. Neurokinin-1R (SP Receptor) Antagonists for HIV Therapy
    Steven Douglas; Fiscal Year: 2008
    ....
  24. Gene Expression Control by the 1918 Flu in Macaques
    CAROLE BASKIN; Fiscal Year: 2008
    ..Such knowledge may lead to new therapeutic or vaccine strategies, which may serve as vital countermeasures against future influenza outbreaks. [unreadable] [unreadable] [unreadable]..
  25. Small Molecule Inhibitors of K13-Induced NF-kB Activation
    PREET CHAUDHARY; Fiscal Year: 2008
    ..Such inhibitors will lead to a better understanding of the K13 protein in the pathogenesis of KSHV-associated cancers and to the development of more effective drugs for their treatment. [unreadable] [unreadable] [unreadable]..
  26. Role and Cell Cycle Mechanisms of pp71 in HCMV Infection
    ROBERT KALEJTA; Fiscal Year: 2007
    ..This information should allow us to design antiviral therapies targeted to the functions of pp71 required for viral replication to treat both proliferative and non-proliferative diseases associated with HCMV infection. ..
  27. Entry of HHV-8 Into the Target Cells
    Bala Chandran; Fiscal Year: 2009
    ..Further understanding of HHV-8-entry, integrin-dependent cell signaling pathways, and their role in infection would eventually lead to the development of new anti-HHV-8 agents. ..
  28. Repression of HIV Transcription: from mechanism to anti-latency therapies
    David Margolis; Fiscal Year: 2007
    ..abstract_text> ..
  29. The Role of CXCL9 in Genital HSV-2 Infection
    Daniel Carr; Fiscal Year: 2009
    ..g., vaccine or gene transfer) to reduce the incidence of infection or reduce the capacity of latent virus to reactivate. ..
  30. Control of cap-independent translation by a viral 3' UTR
    WYATT MILLER; Fiscal Year: 2007
    ..g. dengue, West Nile) that regulate gene expression and replication by long-distance RNA base pairing. Finally, the research will provide fundamental insight on eukaryotic translation initiation mechanisms. ..
  31. Chemopreventive treatment of familial melanoma
    ISABELLE LE POOLE; Fiscal Year: 2008
    ..3] To quantify melanoma tumor growth and measure immunologic parameters (cytokine secretion by ELISA and ELISPOT) in mice treated with bleaching agents and IL-2. [unreadable] [unreadable] [unreadable]..
  32. Role of vFLIP K13 in Bone Marrow Failure Syndrome Associated with Infection by Hu
    PREET CHAUDHARY; Fiscal Year: 2008
    ..This study will lead to a better understanding of the mechanism(s) by which infection with viruses leads to bone marrow failure in transplant patients and provide new treatment strategies. [unreadable] [unreadable] [unreadable]..
  33. AAV and the DNA Damage Machinery
    Matthew D Weitzman; Fiscal Year: 2010
    ..Therefore, in addition to providing insights into the fundamental process of recognition of damaged DNA, these studies will also have implications for genetic approaches to combat disease. ..
  34. HIV/AIDS Intravirion Reverse Transcription and Virucides
    Hui Zhang; Fiscal Year: 2007
    ..These studies are also designed to move analysis of intravirion reverse transcription from the bench towards clinical utility in altering sexual transmission of human lentiviruses. ..
  35. Myosin VIIa Gene Therapy
    Xian Jie Yang; Fiscal Year: 2005
    ..abstract_text> ..
  36. DNA Replication and Genome Integrity
    Matthew Weitzman; Fiscal Year: 2004
    ..abstract_text> ..
  37. Replication and secretion of hepatitis B virus variants
    Shuping Tong; Fiscal Year: 2004
    ..Increase in replication and decrease in secretion may help induce severe liver diseases including fulminant hepatitis. ..
  38. Viral DNA Replication and Late Transcription
    Linda Guarino; Fiscal Year: 2004
    ..Knowledge gained from these experiments should be relevant to other viral systems and will provide a better understanding of the complex interactions between transcription and replication machinery in eukaryotic cells. ..
  39. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    Bruno Sainz; Fiscal Year: 2004
    ..The goal of the studies proposed herein is to elucidate the mechanism(s) by which IFN-beta and IFN-gamma inhibit HSV-1 replication and to identify the cellular factors that are involved in this process. ..
  40. Inhibiting poxvirus phosphatases: therapy and biodefense
    Paula Traktman; Fiscal Year: 2004
    ..Compounds that significantly affect viral infectivity in cellular assays will have high potential for study in animal models and for further development to human therapeutics. ..
  41. REVERSIBLE PROTEIN PHOSPHORYLATION IN APICOMPLEXANS
    Sailen Barik; Fiscal Year: 2004
    ..These results should form the cornerstone in the detailed elucidation of the regulatory pathways and possible drug-target interactions in Plasmodium in particular, and Apicomplexans in general. ..
  42. Genetically stable Picornavirus HIV Immunization vector
    Matthias Gromeier; Fiscal Year: 2004
    ..We will pursue to identify favorable structural characteristics leading to insert retention and efficient expression in our vectors to prepare them for HIV immunization studies in non-human primates. ..
  43. Toll-Like Receptors in Bacterial Antigen Induced HIV-LTR
    Ozlem Equils; Fiscal Year: 2004
    ..abstract_text> ..
  44. EBV AND HHV-8 INTERACTIONS IN PRIMARY EFFUSION LYMPHOMAS
    S Hayward; Fiscal Year: 2004
    ..Aim 3. The HHV-8 latency protein LANA will be characterized to determine its contribution to HHV-8 latency DNA replication and its potential contribution to tumorigenesis through modulation of cellular gene expression. ..
  45. TRANSLATION-INHIBITION OF HEPATITIS C AND POLIO VIRUSES
    Asim Dasgupta; Fiscal Year: 2003
    ..abstract_text> ..
  46. VACCINIA ENCODED KINASES AND PHOSPHATASES
    Paula Traktman; Fiscal Year: 2003
    ....
  47. MODULATION OF HOST CELL GENES BY HIV1 INFECTION
    Jacques Corbeil; Fiscal Year: 2002
    ..In addition, we will determine the modulation of cellular gene expression during the infection of CD4+ T lymphocytes in the context of a X4 or R5 tropism. ..
  48. Chemical Genetics:Cellular Regulators of HCV (RMI)
    Raymond Chung; Fiscal Year: 2004
    ..Second, discovery of hit compounds that positively or negatively regulate HCV replication will ultimately help lead to identification of novel cellular targets that can be exploited to further interrupt the viral lifecycle. ..
  49. Targeting Brain Macrophages by a novel lentiviral vector
    Yuntao Wu; Fiscal Year: 2006
    ..abstract_text> ..
  50. Novel Functions for Telomerase Enzyme Complex in Vivo
    Steven Artandi; Fiscal Year: 2005
    ..Through analysis of a conditional knockout mouse, we will determine the role of endogenous telomerase in cells in culture and in stern/progenitor compartments in vivo. ..
  51. Genes in X-linked Ectodermal Dysplasia Receptor
    PREET CHAUDHARY; Fiscal Year: 2006
    ..In the long-term these studies may lead to better diagnosis and treatment of ectodermal dysplasias and craniofacial abnormalities. ..
  52. Interactions of AAV with Cellular Factors
    Matthew Weitzman; Fiscal Year: 2006
    ..Understanding the role of cellular components in regulating AAV replication and integration will enhance our ability both to produce and utilize this promising vector system. ..
  53. REGULATION OF ALVEOLAR MACROPHAGE FUNCTION
    DAWN FLAHERTY; Fiscal Year: 2005
    ..The studies are important because the shift in phenotype of macrophages in the lung may be critical to the pathogenesis of a number of chronic lung disorders. ..
  54. Stress proteins in autoimmune depigmentation
    ISABELLE LE POOLE; Fiscal Year: 2005
    ..The same model will be of use to test the efficacy of therapeutic modalities to be developed for vitiligo in the future. ..
  55. Intradermal vaccinia:T-cell specificity and skin homing
    David Koelle; Fiscal Year: 2005
    ..2) Measure E-selectin binding by vaccinia-specific CD8 cells, and 2.3) Compare CLA and E-selectin binding by vaccinia-specific T-cells in recent and distant vaccines. ..
  56. ENVELOPE GLYCOPROTEINS OF HHV8
    Bala Chandran; Fiscal Year: 2002
    ..1(A and B) and gB will lead into an important in sight into the development of future control measures. ..
  57. REGULATION OF HEPATIC HEME METABOLISM
    HERBERT BONKOVSKY; Fiscal Year: 2009
    ..the molecular mechanisms that mediate heme regulation of ALAS-1 and HO-1 are similar in avian, human, and murine hepatocytes in culture or in intact organisms. ..
  58. TEXAS REPOSITORY FOR AIDS NEUROPATHOGENESIS RESEARCH
    Benjamin Gelman; Fiscal Year: 2007
    ..abstract_text> ..
  59. Animal models to design & evaluate improved VZV vaccines
    WAYNE GRAY; Fiscal Year: 2007
    ..The study may also provide support for use of the VZV vaccine as a recombinant vector for immunization against other infectious agents, particularly human immunodeficiency virus (HIV). ..
  60. READTHROUGH ACTIVATION OF GENE TRANSCRIPTION
    David Spector; Fiscal Year: 2001
    ..Understanding new molecular mechanisms for controlling gene expression has applications in the development of viral vectors and cellular expression systems for gene therapy. ..
  61. Development of Cellular Assays of Dengue Virus Infection
    Priscilla Yang; Fiscal Year: 2008
    ..These inhibitors can be used to study how dengue virus interacts with its host, and they may also be useful in developing therapies for the treatment of dengue virus infections. [unreadable] [unreadable] [unreadable]..
  62. Development of an VEE Replicon Vaccine Against Smallpox
    Kurt Kamrud; Fiscal Year: 2005
    ....
  63. Tat Interaction with LRP and Its Role in AIDS Dementia
    Johnny He; Fiscal Year: 2006
    ..In addition, these studies will also yield new clues for developing anti-HIV therapeutic strategies for treating HIV-associated neurological disorders occurring in the majority of AIDS patients. ..
  64. MOLECULAR AND GENETIC ANALYSIS OF MVM GENE EXPRESSION
    David Pintel; Fiscal Year: 2008
    ..abstract_text> ..
  65. VSV-based Therapeutic Papilloma Vaccine
    Janet Brandsma; Fiscal Year: 2007
    ..The data provided by this investigation are likely to aid in the development a highly effective HPV vaccine to protect women against cervical cancer. ..
  66. Use a novel Tat transgenic model to develop neuroAIDS therapeutics
    Johnny He; Fiscal Year: 2007
    ..The proposed study is to explore the possibility of using a mouse model to develop, evaluate, and validate therapeutic strategies specifically targeted at these diseases. [unreadable] [unreadable] [unreadable]..
  67. EPSTEIN BARR VIRUS INDUCED GENOMIC INSTABILITY
    John Sixbey; Fiscal Year: 2005
    ....