translational peptide chain termination

Summary

Summary: A process of GENETIC TRANSLATION whereby the last amino acid is added to a lengthening polypeptide. This termination is signaled from the MESSENGER RNA, by one of three termination codons (CODON, TERMINATOR) that immediately follows the last amino acid-specifying CODON.

Top Publications

  1. ncbi Genetic reprogramming by retroviruses: enhanced suppression of translational termination
    Stephen P Goff
    Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA
    Cell Cycle 3:123-5. 2004
  2. ncbi In vitro reconstitution of eukaryotic translation reveals cooperativity between release factors eRF1 and eRF3
    Elena Z Alkalaeva
    Department of Microbiology and Immunology, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
    Cell 125:1125-36. 2006
  3. pmc Termination of translation in eukaryotes is governed by two interacting polypeptide chain release factors, eRF1 and eRF3
    G Zhouravleva
    Departement de Biologie et Genetique du Developpement, CNRS URA 256, Universite de Rennes, France
    EMBO J 14:4065-72. 1995
  4. ncbi A faux 3'-UTR promotes aberrant termination and triggers nonsense-mediated mRNA decay
    Nadia Amrani
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0122, USA
    Nature 432:112-8. 2004
  5. pmc The products of the SUP45 (eRF1) and SUP35 genes interact to mediate translation termination in Saccharomyces cerevisiae
    I Stansfield
    Research School of Biosciences, University of Kent, Canterbury, UK
    EMBO J 14:4365-73. 1995
  6. pmc Distinct eRF3 requirements suggest alternate eRF1 conformations mediate peptide release during eukaryotic translation termination
    Hua Fan-Minogue
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Mol Cell 30:599-609. 2008
  7. pmc Highly conserved NIKS tetrapeptide is functionally essential in eukaryotic translation termination factor eRF1
    Ludmila Frolova
    Engelhardt Institute of Molecular Biology, Moscow, Russia
    RNA 8:129-36. 2002
  8. pmc Complex signals in the genomic 3' nontranslated region of bovine viral diarrhea virus coordinate translation and replication of the viral RNA
    Olaf Isken
    Institute for Cancer Research, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111, USA
    RNA 10:1637-52. 2004
  9. pmc Mechanism of mRNA deadenylation: evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases
    Yuji Funakoshi
    Department of Biological Chemistry, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467 8603, Japan
    Genes Dev 21:3135-48. 2007
  10. pmc Evidence for in vivo ribosome recycling, the fourth step in protein biosynthesis
    L Janosi
    Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA
    EMBO J 17:1141-51. 1998

Research Grants

  1. BIOPHYSICAL AND SCANNING FORCE MICROSCOPY STUDIES
    DOROTHY ERIE; Fiscal Year: 2001
  2. Mechanistic studies of DNA repair and damage response
    Dorothy A Erie; Fiscal Year: 2010
  3. Structure Function Studies of DNA Mismatch Repair
    Dorothy A Erie; Fiscal Year: 2010
  4. Characterization of the SARSCoV frameshift signal
    Jonathan D Dinman; Fiscal Year: 2010
  5. Cell Type-Specific Viral Translation in the CNS
    Matthias Gromeier; Fiscal Year: 2006
  6. Mechanism of Eukaryotic Translation Termination
    David M Bedwell; Fiscal Year: 2011
  7. Regulation of gene expression by ribosomal frameshifting
    JONATHAN DINMAN; Fiscal Year: 2004
  8. RIBOSOMAL FRAMESHIFTING AS A PROBE OF 5S RRNA FUNCTION
    JONATHAN DINMAN; Fiscal Year: 2004
  9. Genetically stable Picornavirus HIV Immunization vector
    Matthias Gromeier; Fiscal Year: 2004
  10. Kinetic Studies of Transcription Elongation
    DOROTHY ERIE; Fiscal Year: 2005

Scientific Experts

Detail Information

Publications143 found, 100 shown here

  1. ncbi Genetic reprogramming by retroviruses: enhanced suppression of translational termination
    Stephen P Goff
    Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA
    Cell Cycle 3:123-5. 2004
    ..The observations suggest that retroviruses manipulate the translational machinery in sophisticated ways to fine-tune their own gene expression...
  2. ncbi In vitro reconstitution of eukaryotic translation reveals cooperativity between release factors eRF1 and eRF3
    Elena Z Alkalaeva
    Department of Microbiology and Immunology, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
    Cell 125:1125-36. 2006
    ..Cooperativity between eRF1 and eRF3 required the eRF3 binding C-terminal domain of eRF1...
  3. pmc Termination of translation in eukaryotes is governed by two interacting polypeptide chain release factors, eRF1 and eRF3
    G Zhouravleva
    Departement de Biologie et Genetique du Developpement, CNRS URA 256, Universite de Rennes, France
    EMBO J 14:4065-72. 1995
    ..It is proposed that a quaternary complex composed of eRF1, eRF3, GTP and a stop codon of the mRNA is involved in termination of polypeptide synthesis in ribosomes...
  4. ncbi A faux 3'-UTR promotes aberrant termination and triggers nonsense-mediated mRNA decay
    Nadia Amrani
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0122, USA
    Nature 432:112-8. 2004
    ..These findings indicate that efficient termination and mRNA stability are dependent on a properly configured 3'-UTR...
  5. pmc The products of the SUP45 (eRF1) and SUP35 genes interact to mediate translation termination in Saccharomyces cerevisiae
    I Stansfield
    Research School of Biosciences, University of Kent, Canterbury, UK
    EMBO J 14:4365-73. 1995
    ....
  6. pmc Distinct eRF3 requirements suggest alternate eRF1 conformations mediate peptide release during eukaryotic translation termination
    Hua Fan-Minogue
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Mol Cell 30:599-609. 2008
    ..These results suggest that the TASNIKS motif and eRF3 function together to trigger eRF1 conformational changes that couple stop codon recognition and peptide release during eukaryotic translation termination...
  7. pmc Highly conserved NIKS tetrapeptide is functionally essential in eukaryotic translation termination factor eRF1
    Ludmila Frolova
    Engelhardt Institute of Molecular Biology, Moscow, Russia
    RNA 8:129-36. 2002
    ..Reduction in ribosome binding revealed for Ile62, Ser64, Arg65, and Arg68 mutants argues in favor of the essential role played by the right part of the NIKS loop in interaction with the ribosome, most probably with ribosomal RNA...
  8. pmc Complex signals in the genomic 3' nontranslated region of bovine viral diarrhea virus coordinate translation and replication of the viral RNA
    Olaf Isken
    Institute for Cancer Research, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111, USA
    RNA 10:1637-52. 2004
    ..Our data suggest the accuracy of translation termination as a sophisticated device determining viral adaptation to the host...
  9. pmc Mechanism of mRNA deadenylation: evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases
    Yuji Funakoshi
    Department of Biological Chemistry, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467 8603, Japan
    Genes Dev 21:3135-48. 2007
    ..Consequently, PABPC1 binding leads to the activation of Pan2-Pan3 and Caf1-Ccr4. From these results, we suggest a mechanism of mRNA deadenylation by Pan2-Pan3 and Caf1-Ccr4 in cooperation with eRF3 and PABPC1...
  10. pmc Evidence for in vivo ribosome recycling, the fourth step in protein biosynthesis
    L Janosi
    Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA
    EMBO J 17:1141-51. 1998
    ..RRF inactivation was bacteriostatic in the growing phase and bactericidal during the transition between the stationary and growing phase, confirming the essential nature of the fourth step of protein synthesis in vivo...
  11. ncbi The essential role of the invariant GGQ motif in the function and stability in vivo of bacterial release factors RF1 and RF2
    Liliana Mora
    UPR9073 du CNRS, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, Paris 75005, France
    Mol Microbiol 47:267-75. 2003
    ....
  12. pmc The stretch of C-terminal acidic amino acids of translational release factor eRF1 is a primary binding site for eRF3 of fission yeast
    K Ito
    Department of Tumor Biology, The Institute of Medical Science, The University of Tokyo, Japan
    RNA 4:958-72. 1998
    ..These results cannot be accounted for by the simple "eRF3-EF-Tu mimicry" model, but may provide new insight into the eRF3 function for translation termination in eukaryotes...
  13. ncbi The crystal structure of human eukaryotic release factor eRF1--mechanism of stop codon recognition and peptidyl-tRNA hydrolysis
    H Song
    Section of Structural Biology, Institute of Cancer Research, London, United Kingdom
    Cell 100:311-21. 2000
    ..A conserved groove on domain 1, 80 A from the GGQ motif, is proposed to form the codon recognition site...
  14. ncbi Structural basis for translation termination on the 70S ribosome
    Martin Laurberg
    Department of Molecular, Cell and Developmental Biology and Center for Molecular Biology of RNA, University of California at Santa Cruz, Santa Cruz, California 95064, USA
    Nature 454:852-7. 2008
    ..Unexpectedly, the main-chain amide group of Gln 230 in the universally conserved GGQ motif of the factor is positioned to contribute directly to peptidyl-tRNA hydrolysis...
  15. ncbi Release factor RF3 abolishes competition between release factor RF1 and ribosome recycling factor (RRF) for a ribosome binding site
    M Y Pavlov
    Department of Molecular Biology, BMC, Uppsala, S 75124, Sweden
    J Mol Biol 273:389-401. 1997
    ..The overproduction of RF1 in cells deficient in RRF or lacking RF3 has effects on growth rate predicted by the in vitro experiments...
  16. ncbi Comparative study of translation termination sites and release factors (RF1 and RF2) in procaryotes
    Y Ozawa
    Institute for Advanced Bioscience, Keio University, 5322 Endo, Fujisawa, Kanagawa 252 8520, Japan
    J Mol Evol 56:665-72. 2003
    ..In addition, some of the residues were located very close to the SPF motif, which deciphers stop codons. These results suggest that these conserved amino acids enable the release factors to recognize the bases around the stop codons...
  17. ncbi Dual functions of ribosome recycling factor in protein biosynthesis: disassembling the termination complex and preventing translational errors
    L Janosi
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    Biochimie 78:959-69. 1996
    ..Finally, we review the known RRF sequences from various organisms including eukaryotes and discuss the possible mechanism for disassembly of the eukaryotic termination complex...
  18. ncbi Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor
    Y Zhang
    Department of Chemistry, Campus Box 3290, University of North Carolina, Chapel Hill, NC 27599 3290, USA
    Biochim Biophys Acta 1443:245-50. 1998
    ..The complete cDNA for the human mitochondrial ribosome recycling factor has been assembled using EST clones. This factor has been expressed in Escherichia coli and purified as a His-tagged protein...
  19. pmc Molecular dissection of translation termination mechanism identifies two new critical regions in eRF1
    Isabelle Hatin
    Université Paris Sud and IGM, CNRS, UMR 8621, Orsay, F 91405, France
    Nucleic Acids Res 37:1789-98. 2009
    ..Our findings are consistent with the model of a closed-active conformation of eRF1 and shed light on two new functional regions of the protein...
  20. pmc Ribosome stalling and peptidyl-tRNA drop-off during translational delay at AGA codons
    Luis Rogelio Cruz-Vera
    Departamento de Genetica y Biologia Molecular, Centro de Investigacion y de Estudios Avanzados del IPN, Apartado Postal 14 740, 07000 Mexico D F
    Nucleic Acids Res 32:4462-8. 2004
    ..These and previous results support the hypothesis that the primary mechanism of inhibition of protein synthesis by AGA triplets in pth+ cells involves sequestration of tRNAs as peptidyl-tRNA on the stalled ribosome...
  21. pmc Ribosome release factor RF4 and termination factor RF3 are involved in dissociation of peptidyl-tRNA from the ribosome
    V Heurgué-Hamard
    UPR9073 du CNRS, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, F 75005 Paris, France
    EMBO J 17:808-16. 1998
    ..It is suggested that RF4 may displace peptidyl-tRNA from the ribosome in a manner related to its proposed function in removing deacylated tRNA during ribosome recycling...
  22. pmc The importance of inter- and intramolecular base pairing for translation reinitiation on a eukaryotic bicistronic mRNA
    Christine Luttermann
    Institut fur Immunologie, Friedrich Loeffler Institut, Tubingen, Germany
    Genes Dev 23:331-44. 2009
    ..Analysis of the essential elements of the TURBS led to a better understanding of the requirements for translation termination/reinitiation in eukaryotes...
  23. pmc Leaky termination at premature stop codons antagonizes nonsense-mediated mRNA decay in S. cerevisiae
    Kim M Keeling
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, USA
    RNA 10:691-703. 2004
    ..5%. This low threshold for the onset of the major component of NMD indicates that mRNA surveillance is an ongoing process that occurs throughout the lifetime of an mRNA...
  24. pmc GTP hydrolysis by eRF3 facilitates stop codon decoding during eukaryotic translation termination
    Joe Salas-Marco
    Department of Microbiology, BBRB 432 Box 8, 1530 Third Ave South, University of Alabama at Birmingham, Birmingham, AL 35294 2170, USA
    Mol Cell Biol 24:7769-78. 2004
    ..Together, these results suggest that the GTPase activity of eRF3 is required to couple the recognition of translation termination signals by eRF1 to efficient polypeptide chain release...
  25. ncbi The eukaryotic polypeptide chain releasing factor (eRF3/GSPT) carrying the translation termination signal to the 3'-Poly(A) tail of mRNA. Direct association of erf3/GSPT with polyadenylate-binding protein
    S Hoshino
    Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113 0033, Japan
    J Biol Chem 274:16677-80. 1999
    ..Thus, the present study is the first report showing that GSPT/eRF3 carries the translation termination signal to 3'-poly(A) tail ubiquitously present in eukaryotic mRNAs...
  26. ncbi Translational termination comes of age
    L L Kisselev
    Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilova, 117984, Moscow, Russia
    Trends Biochem Sci 25:561-6. 2000
    ..Post-termination events in bacteria have been clarified, linking termination, ribosomal recycling and translation initiation...
  27. ncbi Polypeptide chain termination and stop codon readthrough on eukaryotic ribosomes
    S Rospert
    Universitat Freiburg, Institut fur Biochemie und Molekularbiologie, Hermann Herder Strasse 7, 79104 Freiburg, Germany
    Rev Physiol Biochem Pharmacol 155:1-30. 2005
    ..This review explores our current understanding of eukaryotic termination by highlighting the roles of the different ribosomal components as well as termination factors and ribosome-associated proteins, such as chaperones...
  28. ncbi Visualization of release factor 3 on the ribosome during termination of protein synthesis
    Bruno P Klaholz
    Department of Biological Sciences, Imperial College London, London SW7 2AY, UK
    Nature 427:862-5. 2004
    ..Because state 1 seems able to accommodate simultaneously both RF3 and RF2, whose position is known from previous studies, we can infer the release mechanism of class I RFs...
  29. ncbi Translation termination factor aRF1 from the archaeon Methanococcus jannaschii is active with eukaryotic ribosomes
    M Dontsova
    Institute of Protein Research, Russian Academy of Sciences, 142292, Pushchino, Russia
    FEBS Lett 472:213-6. 2000
    ..This observation confirms the earlier prediction that eRF1 and aRF1 form a common structural-functional eRF1/aRF1 protein family, originating from a common ancient ancestor...
  30. pmc Characterization of the termination-reinitiation strategy employed in the expression of influenza B virus BM2 protein
    Michael L Powell
    Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom
    RNA 14:2394-406. 2008
    ..This suggests that the full complement of initiation factors is not required for the reinitiation process...
  31. pmc N-terminal region of Saccharomyces cerevisiae eRF3 is essential for the functioning of the eRF1/eRF3 complex beyond translation termination
    Valery N Urakov
    Institute of Experimental Cardiology, Cardiology Research Center, Moscow, 121552, Russia
    BMC Mol Biol 7:34. 2006
    ..In the yeast Saccharomyces cerevisiae the N-terminal region of eRF3 is responsible for conversion of this protein into the aggregated and functionally inactive prion form...
  32. ncbi Mechanism for the disassembly of the posttermination complex inferred from cryo-EM studies
    Ning Gao
    Wadsworth Center, State University of New York at Albany, Empire State Plaza, Albany, New York 12201, USA
    Mol Cell 18:663-74. 2005
    ..These observations provide the structural basis for the mechanism by which the posttermination complex is split into subunits by the joint action of RRF and EF-G...
  33. ncbi C-terminal domains of human translation termination factors eRF1 and eRF3 mediate their in vivo interaction
    T I Merkulova
    INSERM U248, Institut Curie, Paris, France
    FEBS Lett 443:41-7. 1999
    ..The human eRF1 lacking 22 C-terminal amino acids remains active as a release factor and promotes an eRF3 GTPase activity whereas C-terminally truncated eRF3 is inactive as a GTPase...
  34. ncbi Methylation of bacterial release factors RF1 and RF2 is required for normal translation termination in vivo
    Liliana Mora
    CNRS, UPR 9073, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, Paris 75005, France
    J Biol Chem 282:35638-45. 2007
    ..This suggests that the expression of some genes needed for optimal growth under such conditions can become growth limiting as a result of inefficient translation termination...
  35. ncbi Eukaryotic class 1 translation termination factor eRF1--the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis
    Elena V Ivanova
    Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia
    FEBS J 274:4223-37. 2007
    ....
  36. ncbi Emerging understanding of translation termination
    Y Nakamura
    Department of Tumor Biology, The Institute of Medical Science, The University of Tokyo, Minato ku, Japan
    Cell 87:147-50. 1996
  37. ncbi Ribosomal association of the yeast SAL4 (SUP45) gene product: implications for its role in translation fidelity and termination
    I Stansfield
    Biological Laboratory, University of Kent, Canterbury, UK
    Mol Microbiol 6:3469-78. 1992
    ..In view of these data, and given the stoichiometry of Sal4p to individual ribosomal proteins (estimated at less than 1:20), we suggest that Sal4p plays an ancillary role in translation termination...
  38. ncbi Translational termination-re-initiation in viral systems
    Michael L Powell
    Division of Virology, Department of Pathology, University of Cambridge, Cambridge, UK
    Biochem Soc Trans 36:717-22. 2008
    ....
  39. ncbi Translation termination factors function outside of translation: yeast eRF1 interacts with myosin light chain, Mlc1p, to effect cytokinesis
    I A Valouev
    Institute of Experimental Cardiology, Cardiology Research Center, 3rd Cherepkovskaya Street 15A, 121552 Moscow, Russia
    Mol Microbiol 53:687-96. 2004
    ..The data obtained demonstrate that yeast eRF1 has an important non-translational function effecting cytokinesis via interaction with Mlc1p...
  40. ncbi Class-1 release factor eRF1 promotes GTP binding by class-2 release factor eRF3
    Vasili Hauryliuk
    Department of Cell and Molecular Biology, Molecular Biology Program, BMC, Box 596, Uppsala University, 75124, Sweden
    Biochimie 88:747-57. 2006
    ....
  41. pmc Tpa1p is part of an mRNP complex that influences translation termination, mRNA deadenylation, and mRNA turnover in Saccharomyces cerevisiae
    Kim M Keeling
    Department of Microbiology, BBRB 432 Box 8, 1530 3rd Avenue South, University of Alabama at Birmingham, Birmingham, AL 35294 2170, USA
    Mol Cell Biol 26:5237-48. 2006
    ..These results suggest that Tpa1p is a component of a messenger ribonucleoprotein complex bound to the 3' untranslated region of mRNAs that affects translation termination, deadenylation, and mRNA decay...
  42. pmc A post-translational modification in the GGQ motif of RF2 from Escherichia coli stimulates termination of translation
    V Dincbas-Renqvist
    Department of Cell and Molecular Biology and Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, S 751 24, Uppsala, Sweden
    EMBO J 19:6900-7. 2000
    ..We propose that the previously observed lethal effect of overproducing E.coli K12 RF2 arises through accumulating the defects due to lack of Gln252 methylation and Thr246 in place of alanine...
  43. ncbi The DEAD-box RNA helicase Dbp5 functions in translation termination
    Thomas Gross
    Institut für Molekularbiologie und Tumorforschung der Philipps Universität Marburg, Emil Mannkopff Strasse 2, 35037 Marburg, Germany
    Science 315:646-9. 2007
    ..Therefore, Dbp5 controls the eRF3-eRF1 interaction and thus eRF3-mediated downstream events...
  44. pmc The accuracy of codon recognition by polypeptide release factors
    D V Freistroffer
    Department of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Box 596, S 75124 Uppsala, Sweden
    Proc Natl Acad Sci U S A 97:2046-51. 2000
    ..Two codons, UAU and UGG, stand out as hotspots for RF-dependent premature termination...
  45. ncbi A model for how ribosomal release factors induce peptidyl-tRNA cleavage in termination of protein synthesis
    Stefan Trobro
    Department of Cell and Molecular Biology, Uppsala Biomedical Center, Uppsala University, Box 596, SE 751 24 Uppsala, Sweden
    Mol Cell 27:758-66. 2007
    ..This model explains key mutation experiments and shows that the ribosomal peptidyl transfer center catalyzes its two chemical reactions by a common mechanism...
  46. ncbi The critical role of the universally conserved A2602 of 23S ribosomal RNA in the release of the nascent peptide during translation termination
    Norbert Polacek
    Center for Pharmaceutical Biotechnology m c 870, University of Illinois, 900 S Ashland Avenue, Chicago, IL 60607, USA
    Mol Cell 11:103-12. 2003
    ..This indicates that the mechanism of peptide release is distinct from that of peptide bond formation, with A2602 playing a critical role in peptide release during translation termination...
  47. pmc Fine-tuning of translation termination efficiency in Saccharomyces cerevisiae involves two factors in close proximity to the exit tunnel of the ribosome
    Isabelle Hatin
    IGM, Universite Paris Sud, UMR 8621, F91405 Orsay, France
    Genetics 177:1527-37. 2007
    ..Among them, SSB1 and snR18, two factors close to the exit tunnel of the ribosome, directed the strongest antisuppression effects when overexpressed, showing that they may be involved in fine-tuning of the translation termination level...
  48. pmc Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs
    H Beier
    Institut fur Biochemie, Bayerische Julius Maximilians Universität, Biozentrum, Am Hubland, D 97074 Wurzburg, Germany
    Nucleic Acids Res 29:4767-82. 2001
    ..It is discussed that not only RNA viruses, but also the eukaryotic host organism might gain some profit from cellular suppressor tRNAs...
  49. ncbi Discrimination between defects in elongation fidelity and termination efficiency provides mechanistic insights into translational readthrough
    Joe Salas-Marco
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294 2170, USA
    J Mol Biol 348:801-15. 2005
    ..We used this misincorporation reporter in conjunction with a readthrough reporter system to show that alterations at different regions of the ribosome influence elongation fidelity and termination efficiency to different extents...
  50. ncbi A newly discovered function for RNase L in regulating translation termination
    Florence Le Roy
    Department of Molecular Genetics, Microbiology and Immunology, UMDNJ Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Nat Struct Mol Biol 12:505-12. 2005
    ..On the basis of our results, we present a model describing how RNase L is involved in regulating gene expression by modulating the translation termination process...
  51. ncbi The molecular mechanics of eukaryotic translation
    Lee D Kapp
    Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205 2185, USA
    Annu Rev Biochem 73:657-704. 2004
    ..We discuss the mechanisms conserved across the three kingdoms of life as well as the important divergences that have taken place in the pathway...
  52. ncbi A conserved base-pair between tRNA and 23 S rRNA in the peptidyl transferase center is important for peptide release
    Jason S Feinberg
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093 0314, USA
    J Mol Biol 364:1010-20. 2006
    ....
  53. ncbi Shutdown in protein synthesis due to the expression of mini-genes in bacteria
    V Dincbas
    Department of Cell and Molecular Biology, BMC, Uppsala, S 75124, Sweden
    J Mol Biol 291:745-59. 1999
    ..It is proposed that the retranslation by ribosomes of mini-gene transcripts with efficient ribosome binding (Shine/Dalgarno) sequences strongly contributes to the inhibitory effects of mini-gene expression on protein synthesis...
  54. pmc Termination of translation in eukaryotes is mediated by the quaternary eRF1*eRF3*GTP*Mg2+ complex. The biological roles of eRF3 and prokaryotic RF3 are profoundly distinct
    Vladimir A Mitkevich
    Engelhardt Institute of Molecular Biology, The Russian Academy of Sciences, Moscow 119991, Russia
    Nucleic Acids Res 34:3947-54. 2006
    ..The guanine nucleotide binding properties of eRF3 and of the eRF3*eRF1 complex profoundly differ from those of prokaryotic RF3...
  55. pmc Common and specific amino acid residues in the prokaryotic polypeptide release factors RF1 and RF2: possible functional implications
    Nina J Oparina
    Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street 32, Moscow 119991, Russia
    Nucleic Acids Res 33:5226-34. 2005
    ..Presumably, they also take part in stop codon binding and discrimination. Elucidation of potential functional role(s) of the newly identified SDP/IR zones requires further experiments...
  56. ncbi Polypeptide chain release factors
    R H Buckingham
    CNRS UPR9073, Institut de Biologie Physico Chimique, Paris, France
    Mol Microbiol 24:449-56. 1997
    ..e. RF3 and eRF3...
  57. ncbi Translation termination and yeast prions
    L L Kisselev
    Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 117984, Russia
    Biochemistry (Mosc) 64:1337-41. 1999
    ..This issue of Biochemistry (Moscow) highlights from various points of view the problem of translation termination, excluding protein folding. Yeast termination factors with prion-like properties are also considered...
  58. ncbi Functional interaction between release factor one and P-site peptidyl-tRNA on the ribosome
    S Zhang
    Department of Microbiology Stockholm University, Sweden
    J Mol Biol 261:98-107. 1996
    ..The data suggest that RF1-mediated termination at UAG is sensitive to the nature of the codon-anticodon interaction of the wobble base, the last amino acid residue of the nascent peptide chain, and the tRNA at the ribosomal P-site...
  59. pmc Effects of release factor context at UAA codons in Escherichia coli
    R Martin
    Department of Genetics, University of Washington, Seattle 98195
    J Bacteriol 170:4714-7. 1988
    ..We show here that the activity of RF1 and RF2 varies according to messenger context. RF1 is favored at UAA codons which are efficiently suppressed. RF2 is preferred at poorly suppressed sites...
  60. ncbi The use of fungal in vitro systems for studying translational regulation
    Cheng Wu
    Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, Oregon, USA
    Methods Enzymol 429:203-25. 2007
    ....
  61. pmc Recycling of eukaryotic posttermination ribosomal complexes
    Andrey V Pisarev
    Department of Microbiology and Immunology, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
    Cell 131:286-99. 2007
    ..Its activity is enhanced by eIFs 3j, 1, and 1A. eIF1 also mediates release of P site tRNA, whereas eIF3j ensures subsequent dissociation of mRNA...
  62. pmc A novel mutant of the Sup35 protein of Saccharomyces cerevisiae defective in translation termination and in GTPase activity still supports cell viability
    Celine Fabret
    IGM, Univ Paris Sud, UMR 8621, Orsay, F 91405, France
    BMC Mol Biol 9:22. 2008
    ..The goal of this study was to determine whether this residue is actually phosphorylated in yeast and if it is involved in the termination activity of the protein...
  63. pmc The effect of eukaryotic release factor depletion on translation termination in human cell lines
    Deanna M Janzen
    Division of Human Biology, Fred Hutchinson Cancer Research Center, PO Box 19024, 1100 Fairview Avenue North C2 023, Seattle, WA 98109 1024, USA
    Nucleic Acids Res 32:4491-502. 2004
    ....
  64. pmc Peptide release on the ribosome depends critically on the 2' OH of the peptidyl-tRNA substrate
    Julie L Brunelle
    Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    RNA 14:1526-31. 2008
    ..These observations parallel earlier studies of the mechanism of the peptidyl transfer reaction and argue that related mechanisms are at the heart of catalysis for these reactions...
  65. pmc Transfer-messenger RNA unfolds as it transits the ribosome
    Iwona K Wower
    Department of Animal Sciences, Auburn University, 210 Upchurch Hall, Auburn, AL 36849 5415, USA
    RNA 11:668-73. 2005
    ..Many of these conformational changes are likely to be required during trans-translation to maintain the ribosomal subunits in close proximity to the tmRNA for monitoring its transit...
  66. ncbi Codon-specific and general inhibition of protein synthesis by the tRNA-sequestering minigenes
    Luis Delgado-Olivares
    Departamento de Genetica y Biologia Molecular, Centro de Investigacion y de Estudios Avanzados del IPN, Apartado Postal 14 740, Mexico DF 07000, Mexico
    Biochimie 88:793-800. 2006
    ....
  67. pmc Dissecting eukaryotic translation and its control by ribosome density mapping
    Yoav Arava
    Howard Hughes Medical Institute, Stanford, CA 94305 5428, USA
    Nucleic Acids Res 33:2421-32. 2005
    ..These results provide new insights into eukaryotic translation in vivo...
  68. ncbi Short RNAs repress translation after initiation in mammalian cells
    Christian P Petersen
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Mol Cell 21:533-42. 2006
    ..These results suggest that repression by short RNAs, and thus probably miRNAs, is primarily due to ribosome drop off during elongation of translation...
  69. pmc Comparison of characteristics and function of translation termination signals between and within prokaryotic and eukaryotic organisms
    Andrew G Cridge
    Department of Biochemistry, University of Otago, PO Box 56, Dunedin, New Zealand
    Nucleic Acids Res 34:1959-73. 2006
    ..Termination signals that were highly inefficient could be made more efficient by increasing the concentration of the cognate decoding release factor...
  70. ncbi C-terminal modifications of a protein by UAG-encoded incorporation of puromycin during in vitro protein synthesis in the absence of release factor 1
    Dmitry E Agafonov
    Fellow of the Alexander von Humboldt Foundation on leave from the Institute of Protein Research, Russian Academy of Sciences, Pushchino, Russia
    Chembiochem 7:330-6. 2006
    ..However, the conjugation yield decreased upon addition of more than six nucleotides...
  71. ncbi RNA-protein interactions at the initial and terminal stages of protein biosynthesis as investigated by Lev Kisselev (on the occasion of his 70th anniversary)
    A A Bogdanov
    Lomonosov Moscow State University, Moscow, 119992, Russia
    Biochemistry (Mosc) 71:915-24. 2006
    ....
  72. ncbi Termination-free prokaryotic protein translation by using anticodon-adjusted E. coli tRNASer as unified suppressors of the UAA/UGA/UAG stop codons. Read-through ribosome display of full-length DHFR with translated UTR as a buried spacer arm
    Atsushi Ogawa
    Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo ku, Kyoto 615 8510, Japan
    Chembiochem 7:249-52. 2006
  73. ncbi [Non-canonical decoding events at stop codons in eukaryotes]
    Victoria A Doronina
    Mol Biol (Mosk) 40:731-41. 2006
    ....
  74. pmc Involvement of human release factors eRF3a and eRF3b in translation termination and regulation of the termination complex formation
    Celine Chauvin
    Unite de Biochimie Cellulaire, UMR 7098 CNRS Université Paris 6, 9 quai Saint Bernard, 75252 Paris Cedex 05, France
    Mol Cell Biol 25:5801-11. 2005
    ..Finally, our data indicate that the expression level of eRF3a controls the formation of the termination complex by modulating eRF1 protein stability...
  75. pmc mtRF1a is a human mitochondrial translation release factor decoding the major termination codons UAA and UAG
    Hamid Reza Soleimanpour-Lichaei
    Mitochondrial Research Group, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
    Mol Cell 27:745-57. 2007
    ..Further, mtRF1a depletion in HeLa cells led to compromised growth in galactose and increased production of reactive oxygen species...
  76. pmc Competition between trans-translation and termination or elongation of translation
    Krisana Asano
    Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 036 8561, Japan
    Nucleic Acids Res 33:5544-52. 2005
    ....
  77. ncbi [Characterization of missense mutations in the SUP45 gene of Saccharomyces cerevisiae encoding translation termination factor eRF1]
    S E Moskalenko
    Genetika 40:599-606. 2004
    ..Most of mutations studied do not abolish eRF1-eRF3 interaction. The role of the N-terminal part of eRF1 in stop codon recognition is discussed...
  78. ncbi Ribosome run through of the termination codon in the absence of the ribosome releasing factor
    K Ogawa
    Biochim Biophys Acta 402:288-96. 1975
    ..No such differential effect of suppressor tRNA was observed in the absence of RR factor. This suggests that the ribosome has to be released from mRNA by RR factor in order for the amber mutation to be effective...
  79. ncbi A novel mutation in ribosomal protein S4 that affects the function of a mutated RF1
    A Dahlgren
    Department of Microbiology, Stockholm University, 106 91, Stockholm, Sweden
    Biochimie 82:683-91. 2000
    ....
  80. pmc Release factor RF-3 GTPase activity acts in disassembly of the ribosome termination complex
    G Grentzmann
    Howard Hughes Medical Institute, University of Utah, Salt Lake City 84112, USA
    RNA 4:973-83. 1998
    ..Based on our results, we propose a model of how RF3 might function in translational termination and ribosome recycling...
  81. ncbi Evolution of the eukaryotic translation termination system: origins of release factors
    Y Inagaki
    Program in Evolutionary Biology, Canadian Institute for Advanced Research, Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada
    Mol Biol Evol 17:882-9. 2000
    ..lamblia, and (3) G. lamblia might be the first eukaryotic branch among the organisms considered...
  82. ncbi Instruction of translating ribosome by nascent peptide
    Feng Gong
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Science 297:1864-7. 2002
    ..The location of tryptophan-12 of nascent TnaC in the peptide exit tunnel was crucial for induction. These results show that a nascent peptide sequence can influence translation continuation and termination within a translating ribosome...
  83. ncbi Reinitiation and recycling are distinct processes occurring downstream of translation termination in yeast
    Lukas Rajkowitsch
    Posttranscriptional Control Group, Department of Biomolecular Sciences, UMIST, P O Box 88, M60 1QD, Manchester, UK
    J Mol Biol 335:71-85. 2004
    ....
  84. pmc Divergent tRNA-like element supports initiation, elongation, and termination of protein biosynthesis
    Eric Jan
    Department of Microbiology and Immunology, Stanford University School of Medicine, 299 Campus Drive, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 100:15410-5. 2003
    ..Thus, this P-site-occupying IRES directs the assembly of 80S ribosomes, sets the translational reading frame, and mimics the functions of both Met-tRNAi and peptidyl tRNA to support elongation and termination...
  85. ncbi Specific inhibition of the termination process of protein synthesis by negamycin
    Y Uehara
    Biochim Biophys Acta 442:251-62. 1976
    ..coli cells was sensitive to negamycin. These results indicate that the inhbitory effect of negamycin on the termination reaction is specific to negamycin and distinct from that of tetracycline and streptomycin...
  86. pmc Antizyme frameshifting as a functional probe of eukaryotic translational termination
    Zemfira N Karamysheva
    Department of Biochemistry II, The Jikei University, School of Medicine, 3 25 8 Nishi shinbashi, Minato ku, Tokyo 105 8461, Japan
    Nucleic Acids Res 31:5949-56. 2003
    ..The system affords a convenient assay for release factor activity and has provided some novel views of the mechanism of antizyme frameshifting...
  87. pmc Primary structure of the ovine hypothalamic luteinizing hormone-releasing factor (LRF) (LH-hypothalamus-LRF-gas chromatography-mass spectrometry-decapeptide-Edman degradation)
    R Burgus
    Proc Natl Acad Sci U S A 69:278-82. 1972
    ..The synthetic decapeptide possesses the same biological activities as the native ovine LRF. The amino-acid sequence of ovine LRF is identical to that already published for porcine LRF...
  88. pmc The shuttling protein Npl3 promotes translation termination accuracy in Saccharomyces cerevisiae
    Luis A Estrella
    Department of Biology, University of Puerto Rico, San Juan, Puerto Rico
    J Mol Biol 394:410-22. 2009
    ..Together, these results lead us to propose a model in which Npl3p engenders translational fidelity by promoting the remodeling of mRNPs during translation termination...
  89. ncbi [Viable nonsense mutants for the SUP45 gene in the yeast Saccharomyces cerevisiae are lethal at increased temperature]
    G A Zhuravleva
    Genetika 43:1363-71. 2007
    ..The results of this study suggest that the N-terminal eRF1 fragment is indispensable for cell viability of nonsense mutants due to the involvement in termination of translation...
  90. pmc Mouse haemoglobin beta chains. Comparative sequence data on adult major and minor beta chains from two species, Mus musculus and Mus cervicolor
    J G Gilman
    Biochem J 159:43-53. 1976
    ..K., from whom copies can be obtained on the terms indicated in Biochem. J. (1976) 153, 5...
  91. pmc Ribosomal release without peptidyl tRNA hydrolysis at translation termination in a eukaryotic system
    J Cao
    Division of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    RNA 4:181-8. 1998
    ..These results suggest that a eukaryotic cellular mechanism exists for removing stalled ribosomes from mRNAs in the absence of peptidyl tRNA hydrolysis...
  92. pmc Three distinct peptides from the N domain of translation termination factor eRF1 surround stop codon in the ribosome
    Konstantin N Bulygin
    Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia
    RNA 16:1902-14. 2010
    ..Thus, in the A-site-bound state, the eRF1 conformation significantly differs from those in crystals and solution. The model suggested for eRF1 conformation in the ribosomal A site and cross-linking data are compatible...
  93. ncbi Evolution of translational machinery: could translation termination come into being before elongation?
    Vasili Hauryliuk
    J Theor Biol 248:574-8. 2007
  94. ncbi The urea amidolyase (DUR1,2) gene of Saccharomyces cerevisiae
    F S Genbauffe
    Department of Microbiology and Immunology, University of Tennessee, Memphis 38163
    DNA Seq 2:19-32. 1991
    ..The termini of minor protection fragments also occur at eleven other positions as well...
  95. ncbi Structural transitions induced by the interaction between tRNA(Gly) and the Bacillus subtilis glyQS T box leader RNA
    Mary R Yousef
    Department of Microbiology, The Ohio State University, Columbus, OH 43210, USA
    J Mol Biol 349:273-87. 2005
    ..This study provides biochemical evidence for direct interaction of tRNA(Gly) with full-length in vitro transcribed glyQS leader RNA, and an initial view of structural modulations of both RNA partners within the complex...
  96. ncbi A cytoplasmic protein-protein interaction detection method based on reporter translation
    Laurence Renaut
    MilleGen, Immeuble BIOSTEP, 31681 Labege Cedex, France
    Anal Biochem 384:362-4. 2009
    ..This novel cytoplasmic protein-protein interaction (CPPI) detection system should prove to be useful in the characterization of proteins as well as in partner identification, interaction mapping, and drug discovery applications...
  97. ncbi Structural studies of elongation and release factors
    C G Noble
    Laboratory of Macromolecular Structure, Institute of Molecular and Cell Biology, A STAR Agency for Science, Technology and Research, 61 Biopolis Drive, Proteos, Singapore, Singapore
    Cell Mol Life Sci 65:1335-46. 2008
    ....
  98. ncbi Ribosome structure and dynamics during translocation and termination
    Jack A Dunkle
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    Annu Rev Biophys 39:227-44. 2010
    ..Finally, we review recent advances in understanding how bacteria handle errors in both translocation and termination...
  99. pmc Structure of the 70S ribosome bound to release factor 2 and a substrate analog provides insights into catalysis of peptide release
    Hong Jin
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    Proc Natl Acad Sci U S A 107:8593-8. 2010
    ....
  100. ncbi Role of proteasomes in transcription and their regulation by covalent modifications
    Alexey G Mittenberg
    Institute of Cytology, Russian Academy of Sciences, 4, Tikhoretsky Ave, Saint Petersburg, 194064, Russia
    Front Biosci 13:7184-92. 2008
    ..Here, we review the data on the role of proteasomes in gene regulation and their post-translational modifications as well as discuss potential mechanisms by which proteasomal activity may be regulated by genotoxic stress...
  101. ncbi How lac repressor binds to DNA
    K Adler
    Nature 237:322-7. 1972

Research Grants14

  1. BIOPHYSICAL AND SCANNING FORCE MICROSCOPY STUDIES
    DOROTHY ERIE; Fiscal Year: 2001
    ..3) How do polymorphisms affect the above properties? ..
  2. Mechanistic studies of DNA repair and damage response
    Dorothy A Erie; Fiscal Year: 2010
    ..abstract_text> ..
  3. Structure Function Studies of DNA Mismatch Repair
    Dorothy A Erie; Fiscal Year: 2010
    ....
  4. Characterization of the SARSCoV frameshift signal
    Jonathan D Dinman; Fiscal Year: 2010
    ....
  5. Cell Type-Specific Viral Translation in the CNS
    Matthias Gromeier; Fiscal Year: 2006
    ..This principle has shown great promise in pre-clinical studies in experimental animals and in primary explant cultures of human CNS tumors. ..
  6. Mechanism of Eukaryotic Translation Termination
    David M Bedwell; Fiscal Year: 2011
    ..The third aim of this proposal will test various aspects of this model so we can better understand the important interplay between translation termination and mRNA stability. ..
  7. Regulation of gene expression by ribosomal frameshifting
    JONATHAN DINMAN; Fiscal Year: 2004
    ....
  8. RIBOSOMAL FRAMESHIFTING AS A PROBE OF 5S RRNA FUNCTION
    JONATHAN DINMAN; Fiscal Year: 2004
    ..The proposed research will serve to further our understanding of this ubiquitous yet little understood rRNA and will place us in the unique position to link functional aspects of 5S rRNA to its structure within the ribosome. ..
  9. Genetically stable Picornavirus HIV Immunization vector
    Matthias Gromeier; Fiscal Year: 2004
    ..We will pursue to identify favorable structural characteristics leading to insert retention and efficient expression in our vectors to prepare them for HIV immunization studies in non-human primates. ..
  10. Kinetic Studies of Transcription Elongation
    DOROTHY ERIE; Fiscal Year: 2005
    ..Accordingly, we will use this information to begin to understand, at the amino acid level, the role of NTP binding and conformational transitions in the regulation of elongation. ..
  11. 2002 Gordon Research Conference on Biopolymers
    DOROTHY ERIE; Fiscal Year: 2002
    ..The program encompasses some of the most exciting research in the field of nucleic acid and protein biophysics and promises to generate lively discussions both in and outside of the sessions. ..
  12. Translational control of LTP
    EMMANUEL LANDAU; Fiscal Year: 2007
    ..The results of this proposal will shed light on the mechanisms that participate in drug addiction, as well as learning and memory, by providing new information on the role of translation control in neuronal plasticity. ..
  13. CONSTRUCTION AND ANALYSIS OF RETROVIRUS MUTANTS
    Stephen Goff; Fiscal Year: 2003
    ..In each case, these tests will be applied to existing panels of mutants to help localize the regions needed for these activities. ..