protein biosynthesis

Summary

Summary: The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.

Top Publications

  1. ncbi Upstream and downstream of mTOR
    Nissim Hay
    Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, 60607, USA
    Genes Dev 18:1926-45. 2004
  2. ncbi Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight?
    Witold Filipowicz
    Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland
    Nat Rev Genet 9:102-14. 2008
  3. pmc Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profiling
    Nicholas T Ingolia
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, and California Institute for Quantitative Biosciences, San Francisco, CA 94158, USA
    Science 324:218-23. 2009
  4. ncbi Regulation of mRNA translation and stability by microRNAs
    Marc Robert Fabian
    Department of Biochemistry and Goodman Cancer Research Center, McGill University, Montreal, Quebec, H3G 1Y6, Canada
    Annu Rev Biochem 79:351-79. 2010
  5. ncbi The mechanism of eukaryotic translation initiation and principles of its regulation
    Richard J Jackson
    Department of Biochemistry, University of Cambridge, Cambridge, CB2 1GA, UK
    Nat Rev Mol Cell Biol 11:113-27. 2010
  6. pmc Ribosome profiling of mouse embryonic stem cells reveals the complexity and dynamics of mammalian proteomes
    Nicholas T Ingolia
    Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA
    Cell 147:789-802. 2011
  7. ncbi Switching from repression to activation: microRNAs can up-regulate translation
    Shobha Vasudevan
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    Science 318:1931-4. 2007
  8. pmc Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells
    Yuichi Taniguchi
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 329:533-8. 2010
  9. ncbi Widespread changes in protein synthesis induced by microRNAs
    Matthias Selbach
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, D 13125 Berlin, Germany
    Nature 455:58-63. 2008
  10. pmc Coding-sequence determinants of gene expression in Escherichia coli
    Grzegorz Kudla
    Department of Biology and Program in Applied Mathematics and Computational Science, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 324:255-8. 2009

Research Grants

Detail Information

Publications295 found, 100 shown here

  1. ncbi Upstream and downstream of mTOR
    Nissim Hay
    Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, 60607, USA
    Genes Dev 18:1926-45. 2004
    ..We also summarize the roles of mTOR in the control of cell growth and proliferation, as well as its relevance to cancer and synaptic plasticity...
  2. ncbi Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight?
    Witold Filipowicz
    Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland
    Nat Rev Genet 9:102-14. 2008
    ..This Review summarizes the current understanding of the mechanistic aspects of microRNA-induced repression of translation and discusses some of the controversies regarding different modes of microRNA function...
  3. pmc Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profiling
    Nicholas T Ingolia
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, and California Institute for Quantitative Biosciences, San Francisco, CA 94158, USA
    Science 324:218-23. 2009
    ..Ribosome profiling is readily adaptable to other organisms, making high-precision investigation of protein translation experimentally accessible...
  4. ncbi Regulation of mRNA translation and stability by microRNAs
    Marc Robert Fabian
    Department of Biochemistry and Goodman Cancer Research Center, McGill University, Montreal, Quebec, H3G 1Y6, Canada
    Annu Rev Biochem 79:351-79. 2010
    ..We also address the issue of cellular localization of miRNA-mediated events and a role for RNA-binding proteins in activation or relief of miRNA repression...
  5. ncbi The mechanism of eukaryotic translation initiation and principles of its regulation
    Richard J Jackson
    Department of Biochemistry, University of Cambridge, Cambridge, CB2 1GA, UK
    Nat Rev Mol Cell Biol 11:113-27. 2010
    ....
  6. pmc Ribosome profiling of mouse embryonic stem cells reveals the complexity and dynamics of mammalian proteomes
    Nicholas T Ingolia
    Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA
    Cell 147:789-802. 2011
    ..We also define a class of short, polycistronic ribosome-associated coding RNAs (sprcRNAs) that encode small proteins. Our studies reveal an unanticipated complexity to mammalian proteomes...
  7. ncbi Switching from repression to activation: microRNAs can up-regulate translation
    Shobha Vasudevan
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    Science 318:1931-4. 2007
    ..Thus, activation is a common function of microRNPs on cell cycle arrest. We propose that translation regulation by microRNPs oscillates between repression and activation during the cell cycle...
  8. pmc Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells
    Yuichi Taniguchi
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 329:533-8. 2010
    ..At high expression levels, the distributions are dominated by extrinsic noise. We found that a single cell's protein and mRNA copy numbers for any given gene are uncorrelated...
  9. ncbi Widespread changes in protein synthesis induced by microRNAs
    Matthias Selbach
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, D 13125 Berlin, Germany
    Nature 455:58-63. 2008
    ..Finally, our data suggest that a miRNA can, by direct or indirect effects, tune protein synthesis from thousands of genes...
  10. pmc Coding-sequence determinants of gene expression in Escherichia coli
    Grzegorz Kudla
    Department of Biology and Program in Applied Mathematics and Computational Science, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 324:255-8. 2009
    ..In our analysis, mRNA folding and associated rates of translation initiation play a predominant role in shaping expression levels of individual genes, whereas codon bias influences global translation efficiency and cellular fitness...
  11. pmc Synonymous but not the same: the causes and consequences of codon bias
    Joshua B Plotkin
    Department of Biology and Program in Applied Mathematics and Computational Science, University of Pennsylvania, 433 South University Avenue, Philadelphia, Pennsylvania 19104, USA
    Nat Rev Genet 12:32-42. 2011
    ..Ongoing work to quantify the dynamics of initiation and elongation is as important for understanding natural synonymous variation as it is for designing transgenes in applied contexts...
  12. pmc Mistranslation-induced protein misfolding as a dominant constraint on coding-sequence evolution
    D Allan Drummond
    FAS Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA
    Cell 134:341-52. 2008
    ....
  13. ncbi A "silent" polymorphism in the MDR1 gene changes substrate specificity
    Chava Kimchi-Sarfaty
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Science 315:525-8. 2007
    ....
  14. ncbi Control of translation and mRNA degradation by miRNAs and siRNAs
    Marco Antonio Valencia-Sanchez
    Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, University of Arizona, Tucson, Arizona 85721, USA
    Genes Dev 20:515-24. 2006
    ..An emerging theme is that miRNAs, and siRNAs to some extent, target mRNAs to the general eukaryotic machinery for mRNA degradation and translation control...
  15. pmc The anti-Shine-Dalgarno sequence drives translational pausing and codon choice in bacteria
    Gene Wei Li
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, California 94158, USA
    Nature 484:538-41. 2012
    ..Our results indicate that internal SD-like sequences are a major determinant of translation rates and a global driving force for the coding of bacterial genomes...
  16. pmc CHOP induces death by promoting protein synthesis and oxidation in the stressed endoplasmic reticulum
    Stefan J Marciniak
    Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, New York 10016, USA
    Genes Dev 18:3066-77. 2004
    ..We find that CHOP directly activates GADD34, which promotes ER client protein biosynthesis by dephosphorylating phospho-Ser 51 of the alpha-subunit of translation initiation factor 2 (eIF2alpha) in ..
  17. ncbi Protein translation and folding are coupled by an endoplasmic-reticulum-resident kinase
    H P Harding
    Skirball Institute of Biomolecular Medicine, Department of Medicine, NYU School of Medicine, New York 10016, USA
    Nature 397:271-4. 1999
    ..These properties implicate PERK in a signalling pathway that attenuates protein translation in response to ER stress...
  18. ncbi An evolutionarily conserved mechanism for controlling the efficiency of protein translation
    Tamir Tuller
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Cell 141:344-54. 2010
    ..We suggest that the slow "ramp" at the beginning of mRNAs serves as a late stage of translation initiation, forming an optimal and robust means to reduce ribosomal traffic jams, thus minimizing the cost of protein expression...
  19. pmc Ribosome profiling shows that miR-430 reduces translation before causing mRNA decay in zebrafish
    Ariel A Bazzini
    Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA
    Science 336:233-7. 2012
    ..These results show that miR-430 regulates translation initiation before inducing mRNA decay during zebrafish development...
  20. ncbi MicroRNA-10a binds the 5'UTR of ribosomal protein mRNAs and enhances their translation
    Ulf Andersson Ørom
    Biotech Research and Innovation Centre, University of Copenhagen, Ole Maaløes Vej 5, DK 2200, Copenhagen, Denmark
    Mol Cell 30:460-71. 2008
    ..The results show that miR-10a may positively control global protein synthesis via the stimulation of ribosomal protein mRNA translation and ribosome biogenesis and hereby affect the ability of cells to undergo transformation...
  21. pmc A unifying model for mTORC1-mediated regulation of mRNA translation
    Carson C Thoreen
    Department of Cancer Biology, Dana Farber Cancer Institute, 250 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 485:109-13. 2012
    ..Our results clarify the translational program controlled by mTORC1 and identify 4E-BPs and eIF4G1 as its master effectors...
  22. pmc Human MicroRNA targets
    Bino John
    Computational Biology Center, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    PLoS Biol 2:e363. 2004
    ..microrna.org. Our analysis suggests that miRNA genes, which are about 1% of all human genes, regulate protein production for 10% or more of all human genes...
  23. pmc The impact of microRNAs on protein output
    Daehyun Baek
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 455:64-71. 2008
    ..The impact of microRNAs on the proteome indicated that for most interactions microRNAs act as rheostats to make fine-scale adjustments to protein output...
  24. ncbi Zebrafish MiR-430 promotes deadenylation and clearance of maternal mRNAs
    Antonio J Giraldez
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
    Science 312:75-9. 2006
    ..We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis...
  25. pmc FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism
    Jennifer C Darnell
    Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, NY 10065, USA
    Cell 146:247-61. 2011
    ..In addition, they provide insight into the molecular basis of the cognitive and allied defects in FXS and ASD and suggest multiple targets for clinical intervention...
  26. ncbi Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10021, USA
    Mol Cell 15:185-97. 2004
    ..The specific role of Ago2 in guiding target RNA cleavage was confirmed independently by siRNA-based depletion of individual Ago members in combination with a sensitive positive-readout reporter assay...
  27. ncbi Translational regulation of gene expression during conditions of cell stress
    Keith A Spriggs
    Centre for Biomolecular Sciences, School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, UK
    Mol Cell 40:228-37. 2010
    ..Translational reprogramming can result in increased life span, and conversely, deregulation of these translation pathways is associated with disease including cancer and diabetes...
  28. pmc Stress granules and processing bodies are dynamically linked sites of mRNP remodeling
    Nancy Kedersha
    Division of Rheumatology and Immunology, Harvard Medical School, Brigham and Women s Hospital, Boston, MA 02115, USA
    J Cell Biol 169:871-84. 2005
    ..We propose that mRNA released from disassembled polysomes is sorted and remodeled at SGs, from which selected transcripts are delivered to PBs for degradation...
  29. pmc Eukaryotic stress granules: the ins and outs of translation
    J Ross Buchan
    Howard Hughes Medical Institute, University of Arizona, Tucson, 85721, USA
    Mol Cell 36:932-41. 2009
    ..Together, stress granules and P-bodies reveal a dynamic cycle of distinct biochemical and compartmentalized mRNPs in the cytosol, with implications for the control of mRNA function...
  30. pmc Automated design of synthetic ribosome binding sites to control protein expression
    Howard M Salis
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, USA
    Nat Biotechnol 27:946-50. 2009
    ..The proposed forward engineering approach should accelerate the construction and systematic optimization of large genetic systems...
  31. pmc Target mRNAs are repressed as efficiently by microRNA-binding sites in the 5' UTR as in the 3' UTR
    J Robin Lytle
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06536, USA
    Proc Natl Acad Sci U S A 104:9667-72. 2007
    ..Our results suggest that association with any position on a target mRNA is mechanistically sufficient for a microribonucleoprotein to exert repression of translation at some step downstream of initiation...
  32. ncbi Correlation of mRNA and protein in complex biological samples
    Tobias Maier
    Center for Genomic Regulation, Barcelona, Spain
    FEBS Lett 583:3966-73. 2009
    ..We review available literature on the correlation of mRNA and protein abundances and discuss biological and technical parameters influencing the correlation of these central biological molecules...
  33. ncbi Translational regulation of GCN4 and the general amino acid control of yeast
    Alan G Hinnebusch
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Annu Rev Microbiol 59:407-50. 2005
    ..Remarkably, mammalian cells use the same strategy to downregulate protein synthesis while inducing transcriptional activators of stress-response genes under various stressful conditions, including amino acid starvation...
  34. ncbi Translational control in cancer
    Deborah Silvera
    Department of Microbiology, New York, New York 10016, USA
    Nat Rev Cancer 10:254-66. 2010
    ..Several clinical efforts are underway to target specific components of the translation apparatus or unique mRNA translation elements for cancer therapeutics...
  35. ncbi Selective translation of leaderless mRNAs by specialized ribosomes generated by MazF in Escherichia coli
    Oliver Vesper
    Max F Perutz Laboratories, Center for Molecular Biology, Department of Microbiology, Immunobiology and Genetics, University of Vienna, Dr Bohrgasse 9 4, 1030 Vienna, Austria
    Cell 147:147-57. 2011
    ..Thus, we have discovered a modified translation machinery that is generated in response to MazF induction and that probably serves for stress adaptation in Escherichia coli...
  36. ncbi Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans
    B Wightman
    Department of Molecular Biology, Massachusetts General Hospital, Boston 02114
    Cell 75:855-62. 1993
    ..These data suggest a molecular mechanism for Lin-14p temporal gradient formation: the lin-4 RNAs base pair to sites in the lin-14 3'UTR to form multiple RNA duplexes that down-regulate lin-14 translation...
  37. pmc Balanced codon usage optimizes eukaryotic translational efficiency
    Wenfeng Qian
    Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan, United States of America
    PLoS Genet 8:e1002603. 2012
    ....
  38. ncbi Crystal structure of the eukaryotic 40S ribosomal subunit in complex with initiation factor 1
    Julius Rabl
    Institute of Molecular Biology and Biophysics, ETH Zurich, Schafmattstrasse 20, 8093 Zurich, Switzerland
    Science 331:730-6. 2011
    ..It provides insights into the eukaryotic-specific aspects of protein synthesis, including the function of eIF1 as well as signaling and regulation mediated by the ribosomal proteins RACK1 and rpS6e...
  39. ncbi The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans
    B J Reinhart
    Department of Molecular Biology, Massachusetts General Hospital, and Harvard Medical School, Boston 02114, USA
    Nature 403:901-6. 2000
    ..We propose that the sequential stage-specific expression of the lin-4 and let-7 regulatory RNAs triggers transitions in the complement of heterochronic regulatory proteins to coordinate developmental timing...
  40. ncbi Noise in protein expression scales with natural protein abundance
    Arren Bar-Even
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Nat Genet 38:636-43. 2006
    ....
  41. ncbi Stress signaling from the lumen of the endoplasmic reticulum: coordination of gene transcriptional and translational controls
    R J Kaufman
    Department of Biological Chemistry and Howard Hughes Medical Institute, The University of Michigan Medical Center, Ann Arbor, Michigan 48109 0650 USA
    Genes Dev 13:1211-33. 1999
  42. pmc Feedback inhibition of the unfolded protein response by GADD34-mediated dephosphorylation of eIF2alpha
    I Novoa
    Skirball Institute of Biomolecular Medicine, Department of Medicine, New York University School of Medicine, New York, NY 10016, USA
    J Cell Biol 153:1011-22. 2001
    ....
  43. pmc Solving the riddle of codon usage preferences: a test for translational selection
    Mario dos Reis
    School of Crystallography, Birkbeck College, University of London, Malet Street, London WC1E 7HX, UK
    Nucleic Acids Res 32:5036-44. 2004
    ..Helicobacter pylori, Saccharomyces cerevisiae and Homo sapiens are codon usage paradigms that can be better understood under the proposed model...
  44. pmc High-resolution view of the yeast meiotic program revealed by ribosome profiling
    Gloria A Brar
    Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA
    Science 335:552-7. 2012
    ..This work reveals pervasive translational control in meiosis and helps to illuminate the molecular basis of the broad restructuring of meiotic cells...
  45. pmc Global signatures of protein and mRNA expression levels
    Raquel de Sousa Abreu
    Children s Cancer Research Institute, University of Texas Health Science Center at San Antonio, TX, USA
    Mol Biosyst 5:1512-26. 2009
    ..We summarize the information that can be derived from comparison of protein and mRNA expression levels and discuss how corresponding sequence characteristics suggest modes of regulation...
  46. ncbi Lifespan extension by conditions that inhibit translation in Caenorhabditis elegans
    Malene Hansen
    Department of Biochemistry and Biophysics, University of California, 600 16th Street, San Francisco, CA 94158, USA
    Aging Cell 6:95-110. 2007
    ..Our findings link TOR, but not sir-2.1, to the longevity response induced by dietary restriction (DR) in C. elegans, and they suggest that neither TOR inhibition nor DR extends lifespan simply by reducing protein synthesis...
  47. ncbi A mechanistic overview of translation initiation in eukaryotes
    Colin Echeverría Aitken
    Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Nat Struct Mol Biol 19:568-76. 2012
    ..Here, we outline our current understanding of eukaryotic translation initiation and discuss important outstanding challenges...
  48. ncbi Synonymous codon usage in Escherichia coli: selection for translational accuracy
    Nina Stoletzki
    Ludwig Maximilan Universität, Biocenter, Planegg Martinsried, Germany
    Mol Biol Evol 24:374-81. 2007
    ..Considering each amino acid by itself confirms our results. We conclude that selection on synonymous codon use in E. coli is largely due to selection for translational accuracy, to reduce the costs of both missense and nonsense errors...
  49. ncbi A microRNA in a multiple-turnover RNAi enzyme complex
    Gyorgy Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
    Science 297:2056-60. 2002
    ..Each let-7-containing complex directs multiple rounds of RNA cleavage, which explains the remarkable efficiency of the RNAi pathway in human cells...
  50. ncbi Perk is essential for translational regulation and cell survival during the unfolded protein response
    H P Harding
    Department of Medicine, The Kaplan Cancer Center, New York University School of Medicine, New York 10016, USA
    Mol Cell 5:897-904. 2000
    ..PERK thus plays a major role in the ability of cells to adapt to ER stress...
  51. ncbi Translational control is required for the unfolded protein response and in vivo glucose homeostasis
    D Scheuner
    Howard Hughes Medical Institute, University of Michigan Medical Center, 48109, Ann Arbor, MI, USA
    Mol Cell 7:1165-76. 2001
    ..The results demonstrate that regulation of translation through eIF2alpha phosphorylation is essential for the ER stress response and in vivo glucose homeostasis...
  52. pmc Determinants of translation efficiency and accuracy
    Hila Gingold
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    Mol Syst Biol 7:481. 2011
    ..A practical demonstration of a better understanding of the process would be a more accurate prediction of the proteome, given the transcriptome at a diversity of biological conditions...
  53. pmc Kinetic analysis reveals successive steps leading to miRNA-mediated silencing in mammalian cells
    Julien Béthune
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland
    EMBO Rep 13:716-23. 2012
    ..This step is followed by mRNA deadenylation and decay, which is the dominant effect at steady state. Our findings have important implications for understanding the mechanism of silencing and reconcile seemingly contradictory data...
  54. pmc AU-rich elements and associated factors: are there unifying principles?
    Carine Barreau
    UMR 6061 CNRS Génétique et développement, IFR 140 Génétique Fonctionnelle Agronomie et Santé, Universite de Rennes 1, Faculte de Medecine, CS 34317, 35043 Rennes Cedex, France
    Nucleic Acids Res 33:7138-50. 2005
    ..The potential relationship between mRNA stability and translation is also discussed. Finally, we present several hypotheses that could unify the published data and suggest avenues for future research...
  55. ncbi What recent ribosome structures have revealed about the mechanism of translation
    T Martin Schmeing
    MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    Nature 461:1234-42. 2009
    ..In this review, we discuss how the interaction between structural and functional studies over the last decade has led to a deeper understanding of the complex mechanisms underlying translation...
  56. pmc The evolutionary consequences of erroneous protein synthesis
    D Allan Drummond
    FAS Center for Systems Biology, Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA
    Nat Rev Genet 10:715-24. 2009
    ..and theoretical results on this topic have accumulated rapidly in disparate fields, such as neurobiology, protein biosynthesis and degradation and molecular evolution, but with limited communication among disciplines...
  57. ncbi Crystal structure of the eukaryotic ribosome
    Adam Ben-Shem
    IGBMC Institut de Génétique et de Biologie Moléculaire et Cellulaire, 1 rue Laurent Fries, BP10142, Illkirch F 67400, France
    Science 330:1203-9. 2010
    ..We describe the conformational changes in both ribosomal subunits that are involved in ratcheting and their implications in coordination between the two associated subunits and in mRNA and tRNA translocation...
  58. pmc Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans
    Sarah E Calvo
    Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 106:7507-12. 2009
    ..Together, our results suggest that uORFs influence the protein expression of thousands of mammalian genes and that variation in these elements can influence human phenotype and disease...
  59. ncbi Synonymous mutations in the human dopamine receptor D2 (DRD2) affect mRNA stability and synthesis of the receptor
    Jubao Duan
    Schizophrenia Genetics Research Program, Department of Psychiatry, The University of Chicago, Jules F Knapp Medical Research Center, Chicago, IL 60637, USA
    Hum Mol Genet 12:205-16. 2003
    ....
  60. ncbi Translational control in stress and apoptosis
    Martin Holcik
    Apoptosis Research Center, Room R3116, Children s Hospital of Eastern Ontario, 401 Smyth Road, Ottawa, ON, K1H 8L1, Canada
    Nat Rev Mol Cell Biol 6:318-27. 2005
    ....
  61. pmc Principles of microRNA-target recognition
    Julius Brennecke
    European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS Biol 3:e85. 2005
    ....
  62. pmc Molecular mechanism of scanning and start codon selection in eukaryotes
    Alan G Hinnebusch
    Laboratory of Gene Regulation and Development, Eunice K Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Microbiol Mol Biol Rev 75:434-67, first page of table of contents. 2011
    ....
  63. ncbi A role for codon order in translation dynamics
    Gina Cannarozzi
    Institute of Computational Science, ETH Zurich, 8092 Zurich, Switzerland
    Cell 141:355-67. 2010
    ..They also establish that the dynamics of translation leave a significant signature at the level of the genome...
  64. ncbi Analysis of the aphthovirus 2A/2B polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal 'skip'
    M L Donnelly
    Centre for Biomolecular Sciences, School of Biology, Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK
    J Gen Virol 82:1013-25. 2001
    ..This process produces a ribosomal 'skip' from one codon to the next without the formation of a peptide bond...
  65. pmc Autism-related deficits via dysregulated eIF4E-dependent translational control
    Christos G Gkogkas
    Department of Biochemistry and Goodman Cancer Research Centre, McGill University, Montreal, Quebec H3A 1A3, Canada
    Nature 493:371-7. 2013
    ..Thus, translational control by eIF4E regulates the synthesis of neuroligins, maintaining the excitation-to-inhibition balance, and its dysregulation engenders ASD-like phenotypes...
  66. pmc Reinitiation involving upstream ORFs regulates ATF4 mRNA translation in mammalian cells
    Krishna M Vattem
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Proc Natl Acad Sci U S A 101:11269-74. 2004
    ..These results suggest that the mechanism of translation reinitiation involving uORFs is conserved from yeast to mammals...
  67. ncbi Coping with stress: eIF2 kinases and translational control
    R C Wek
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Biochem Soc Trans 34:7-11. 2006
    ....
  68. pmc Concordant regulation of translation and mRNA abundance for hundreds of targets of a human microRNA
    David G Hendrickson
    Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, California, USA
    PLoS Biol 7:e1000238. 2009
    ....
  69. ncbi Discordant protein and mRNA expression in lung adenocarcinomas
    Guoan Chen
    Department of Surgery, University of Michigan, Ann Arbor, Michigan 48109, USA
    Mol Cell Proteomics 1:304-13. 2002
    ..Using a quantitative analysis of mRNA and protein expression within the same lung adenocarcinomas, we showed that only a subset of the proteins exhibited a significant correlation with mRNA abundance...
  70. pmc Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution
    Jean Paul Armache
    Gene Center, Department of Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Proc Natl Acad Sci U S A 107:19748-53. 2010
    b>Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes...
  71. ncbi The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
    R C Lee
    Harvard University, Department of Cellular and Developmental Biology, Cambridge, Massachusetts 02138
    Cell 75:843-54. 1993
    ..elegans and found to contain sequences complementary to a repeated sequence element in the 3' untranslated region (UTR) of lin-14 mRNA, suggesting that lin-4 regulates lin-14 translation via an antisense RNA-RNA interaction...
  72. pmc Decapping and decay of messenger RNA occur in cytoplasmic processing bodies
    Ujwal Sheth
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    Science 300:805-8. 2003
    ..These results define the flux of mRNAs between polysomes and P bodies as a critical aspect of cytoplasmic mRNA metabolism and a possible site for regulation of mRNA degradation...
  73. ncbi P bodies: at the crossroads of post-transcriptional pathways
    Ana Eulalio
    Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    Nat Rev Mol Cell Biol 8:9-22. 2007
    ....
  74. ncbi Post-transcriptional regulation of gene expression in trypanosomes and leishmanias
    Christine Clayton
    Zentrum für Molekualre Biologie der Universität Heidelberg, Im Neuenheimer Feld 282, D69120 Heidelberg, Germany
    Mol Biochem Parasitol 156:93-101. 2007
    ..Thus the regulation of gene expression is post-transcriptional. We here discuss recent results concerning the enzymes required for mRNA degradation, and components of the translation initiation machinery, and how both are regulated...
  75. pmc A parsimonious model for gene regulation by miRNAs
    Sergej Djuranovic
    Howard Hughes Medical Institute HHMI, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Science 331:550-3. 2011
    ..More systematic analyses of the molecular specificities of the core components coupled with analysis of the relative timing of the different events will ultimately shed light on the mechanism of miRNA-mediated repression...
  76. pmc Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation
    Marc R Fabian
    Department of Biochemistry, McGill University, Montreal, QC H3G 1Y6, Canada
    Mol Cell 35:868-80. 2009
    ..Importantly, we present evidence that GW182, a core component of the miRISC, directly interacts with PABP via its C-terminal region and that this interaction is required for miRNA-mediated deadenylation...
  77. pmc Diverse molecular functions of Hu proteins
    M N Hinman
    Department of Genetics, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, Ohio 44106, USA
    Cell Mol Life Sci 65:3168-81. 2008
    ..In addition, an intriguing theme of antagonism between Hu proteins and other AU-rich sequence binding proteins will be discussed...
  78. pmc microRNA-122 stimulates translation of hepatitis C virus RNA
    Jura Inga Henke
    Institute of Biochemistry, Faculty of Medicine, Justus Liebig University, Giessen, Germany
    EMBO J 27:3300-10. 2008
    ..miR-122 stimulates HCV translation by enhancing the association of ribosomes with the viral RNA at an early initiation stage. In conclusion, the liver-specific miR-122 may contribute to HCV liver tropism at the level of translation...
  79. pmc Cellular IRES-mediated translation: the war of ITAFs in pathophysiological states
    Anton A Komar
    Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences, Cleveland State University, Cleveland, OH, USA
    Cell Cycle 10:229-40. 2011
    ....
  80. ncbi Dendritic protein synthesis, synaptic plasticity, and memory
    Michael A Sutton
    Division of Biology 114 96, California Institute of Technology, Howard Hughes Medical Institute, Pasadena, CA 91125, USA
    Cell 127:49-58. 2006
    ..Here, we review the experimental support for this idea and highlight some of the key questions outstanding in this area...
  81. pmc Polysomes, P bodies and stress granules: states and fates of eukaryotic mRNAs
    Vidya Balagopal
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0206, USA
    Curr Opin Cell Biol 21:403-8. 2009
    ..Here we discuss how these different granules suggest a dynamic model for the metabolism of cytoplasmic mRNAs wherein they cycle between different mRNP states with different functional properties and subcellular locations...
  82. ncbi Translational control of long-lasting synaptic plasticity and memory
    Mauro Costa-Mattioli
    Department of Biochemistry and McGill Cancer Center, McGill University, Montreal QCH3G1Y6, Canada
    Neuron 61:10-26. 2009
    ..In this review, we summarize and discuss recent progress in the field and highlight the prospects for better understanding of long-lasting changes in synaptic strength, learning, and memory and implications for neurological diseases...
  83. pmc Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like protein
    Maria A Schumacher
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR 97201 3098, USA
    EMBO J 21:3546-56. 2002
    ..Such binding suggests a mechanism for Hfq function...
  84. ncbi Synaptic tagging and long-term potentiation
    U Frey
    Federal Institute for Neurobiology, Gene Regulation and Plasticity, Magdeburg, Germany
    Nature 385:533-6. 1997
    ..The synaptic tag decays in less than three hours. These findings indicate that the persistence of LTP depends not only on local events during its induction, but also on the prior activity of the neuron...
  85. pmc The ribosome uses two active mechanisms to unwind messenger RNA during translation
    Xiaohui Qu
    Jason L Choy Laboratory of Single Molecule Biophysics and QB3 Institute, University of California, Berkeley, California 94720, USA
    Nature 475:118-21. 2011
    ..Our results establish a quantitative mechanical basis for understanding the mechanism of regulation of the elongation rate of translation by structured mRNAs...
  86. pmc The DEAD-box protein Ded1 modulates translation by the formation and resolution of an eIF4F-mRNA complex
    Angela Hilliker
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721 0206, USA
    Mol Cell 43:962-72. 2011
    ..These results identify Ded1 as a translation initiation factor that assembles and remodels an intermediate complex in translation initiation...
  87. ncbi Spatial regulation of beta-actin translation by Src-dependent phosphorylation of ZBP1
    Stefan Huttelmaier
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Nature 438:512-5. 2005
    ..These sequential events provide both temporal and spatial control over beta-actin mRNA translation, which is important for cell migration and neurite outgrowth...
  88. pmc BLAT--the BLAST-like alignment tool
    W James Kent
    Department of Biology and Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, CA 95064, USA
    Genome Res 12:656-64. 2002
    ..BLAT is compared with other alignment programs on various test sets and then used in several genome-wide applications. http://genome.ucsc.edu hosts a web-based BLAT server for the human genome...
  89. pmc Regulation of flowering time and floral organ identity by a MicroRNA and its APETALA2-like target genes
    Milo J Aukerman
    E I duPont de Nemours and Company, Agriculture and Nutrition, Newark, Delaware 19711, USA
    Plant Cell 15:2730-41. 2003
    ..Gain-of-function and loss-of-function analyses indicate that two of the AP2-like target genes normally act as floral repressors, supporting the notion that miR172 regulates flowering time by downregulating AP2-like target genes...
  90. pmc 4E-BP extends lifespan upon dietary restriction by enhancing mitochondrial activity in Drosophila
    Brian M Zid
    California Institute of Technology, Pasadena, CA 91125, USA
    Cell 139:149-60. 2009
    ..For a video summary of this article, see the PaperFlick file with the Supplemental Data available online...
  91. pmc S6K1(-/-)/S6K2(-/-) mice exhibit perinatal lethality and rapamycin-sensitive 5'-terminal oligopyrimidine mRNA translation and reveal a mitogen-activated protein kinase-dependent S6 kinase pathway
    Mario Pende
    Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland
    Mol Cell Biol 24:3112-24. 2004
    ..These data reveal a redundancy between the S6K and the MAPK pathways in mediating early S6 phosphorylation in response to mitogens...
  92. pmc The structure of the ribosome with elongation factor G trapped in the posttranslocational state
    Yong Gui Gao
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK
    Science 326:694-9. 2009
    ..The stabilization of the mobile stalks of the ribosome also results in a more complete description of its structure...
  93. pmc Nrf2 is a direct PERK substrate and effector of PERK-dependent cell survival
    Sara B Cullinan
    The Leonard and Madlyn Abramson Family Cancer Research Institute and Cancer Center, Department of Cancer Biology, University of Pennsylvania Cancer Center, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Biol 23:7198-209. 2003
    ..Our data demonstrate that Nrf2 is a critical effector of PERK-mediated cell survival...
  94. pmc Translation reinitiation at alternative open reading frames regulates gene expression in an integrated stress response
    Phoebe D Lu
    Department of Cell Biology, School of Medicine, New York University, New York, NY 10016, USA
    J Cell Biol 167:27-33. 2004
    ..These features are common to regulated translation of GCN4 in yeast. The metazoan ISR thus resembles the yeast general control response both in its target genes and its mechanistic details...
  95. pmc Movement of eukaryotic mRNAs between polysomes and cytoplasmic processing bodies
    Muriel Brengues
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0106, USA
    Science 310:486-9. 2005
    ..Moreover, the presence of related proteins in P-bodies and maternal mRNA storage granules suggests this mechanism is widely adapted for mRNA storage...
  96. pmc Explaining complex codon usage patterns with selection for translational efficiency, mutation bias, and genetic drift
    Premal Shah
    Department of Ecology and Evolutionary Biology, University of Tennessee, Knoxville, TN 37996, USA
    Proc Natl Acad Sci U S A 108:10231-6. 2011
    ....
  97. pmc ERAL1 is associated with mitochondrial ribosome and elimination of ERAL1 leads to mitochondrial dysfunction and growth retardation
    Takeshi Uchiumi
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, 3 1 1, Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Nucleic Acids Res 38:5554-68. 2010
    ..These results suggest that ERAL1 is localized in a small subunit of the mitochondrial ribosome, plays an important role in the small ribosomal constitution, and is also involved in cell viability...
  98. pmc Upstream open reading frames: molecular switches in (patho)physiology
    Klaus Wethmar
    Max Delbrueck Center for Molecular Medicine, Berlin, Germany
    Bioessays 32:885-93. 2010
    ....
  99. ncbi Real-time tRNA transit on single translating ribosomes at codon resolution
    Sotaro Uemura
    Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305 5126, USA
    Nature 464:1012-7. 2010
    ..The methods outlined here have broad application to the study of mRNA sequences, and the mechanism and regulation of translation...
  100. pmc A translational profiling approach for the molecular characterization of CNS cell types
    Myriam Heiman
    Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, NY 10065, USA
    Cell 135:738-48. 2008
    ....
  101. pmc MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms
    Yan Zeng
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 100:9779-84. 2003
    ..These data suggest that miRNAs and siRNAs can use similar mechanisms to repress mRNA expression and that the choice of mechanism may be largely or entirely determined by the degree of complementary of the RNA target...

Research Grants70

  1. Genetic variation and regulatory networks: Mechanisms and complexity
    DANA PE ER; Fiscal Year: 2009
    ..These tools will be made publicly available, including a friendly graphical user interface and visualization. ..
  2. Personalized Clinical Diagnostics and Beyond: Integrated Ring Resonator Arrays
    Ryan Bailey; Fiscal Year: 2009
    ..This technology promises to broadly impact the landscape of the biomedical sciences, both meeting the clinical diagnostic challenges of today and pioneering the paradigm-shifting discoveries of tomorrow. ..
  3. Regulation of ER stress-induced cell death
    Randal J Kaufman; Fiscal Year: 2013
    ..The proposed studies on protein synthesis will provide vital information that should encourage the development of novel therapeutics to treat diabetes. ..
  4. Translational Machinery in Stress Signaling and Tumor Suppression
    THOMAS J contact BEGLEY; Fiscal Year: 2010
    ..The long term potential of the proposed research is significant, as understanding the mechanisms by which TRM9 inhibits tumor growth may provide novel therapeutic opportunities to restore growth-restricting programs in cancer cells. ..
  5. New insights in mechanisms of renal injury
    Balakuntalam S Kasinath; Fiscal Year: 2013
    ..Upstream regulators and downstream effectors of GSK 3beta in transcription and translation pathways involved in matrix protein synthesis will be explored in renal tissues as stated in Aim 1. ..
  6. NITROGEN HETEROCYCLES: METABOLIC EFFECTS AND TOXICITY
    Raymond F Novak; Fiscal Year: 2010
    ....
  7. Biological Functions of Iron Responsive Elements
    Richard S Eisenstein; Fiscal Year: 2012
    ....
  8. Regulation of the Endothelial Citrulline-Nitric Oxide Cycle
    Duane C Eichler; Fiscal Year: 2010
    ..enzyme that is required to maintain vascular wall function is essential to this understanding, and will potentially distinguish new therapeutic strategies for the treatment of diabetes, hypertension and related cardiovascular disease ..
  9. Enterovirus RNA Translation and Replication
    David J Barton; Fiscal Year: 2013
    ..The success of the poliovirus eradication campaign may depend upon improved poliovirus vaccines and new antiviral drugs. ..
  10. PHOSPHOPEPTIDE METABOLISM IN ADIPOCYTES
    Joseph Avruch; Fiscal Year: 2013
    ..We will also determine how TOR complex 1 controls the translation of RNAs that bind to the protein IMP2, variants of whose gene appear to confer susceptibility to type 2 diabetes. ..
  11. Investigation of underlying mechanisms of sex differences in cerebral ischemia
    Chad S Siegel; Fiscal Year: 2010
    ..The overall goal of this proposal is to investigate mechanisms underlying sex differences in ischemic cell death. ..
  12. Biological Functions of Iron Responsive Elements
    Richard S Eisenstein; Fiscal Year: 2013
    ..abstract_text> ..
  13. The survival of motor neuron protein in axonal mRNA localization
    PAUL GREGORY DONLIN-ASP; Fiscal Year: 2013
    ..Through identification of novel mislocalized mRNA transcripts and a role for SMN in mRNA localization, this research will identify novel targets for the development of SMA therapies. ..
  14. SECOND MESSENGERS IN THE RETINA
    RAJU VS RAJALA; Fiscal Year: 2012
    ..Our long-term goal is to understand these mechanisms as a foundation on which novel drug therapies can be designed to protect retinal function in patients who suffer from retinal degenerations. ..
  15. Functional Genomic Dissection of Refractory Anemia
    BENJAMIN LEVINE EBERT; Fiscal Year: 2013
    ..In aggregate, these experiments will provide critical insight into the molecular basis of myelodysplastic syndrome. ..
  16. Coordination of metabolism and virulence during infection
    Tony Romeo; Fiscal Year: 2013
    ..Thus, an understanding of the conditions and stimuli affecting SirA and CsrA activities and the mechanisms by which these proteins coordinate virulence and metabolic gene expression may be applicable to a broad range of pathogens. ..
  17. Suppression of the Interferon Response by the Hepatitis C Virus
    FRANCIS VINCENT CHISARI; Fiscal Year: 2010
    ..abstract_text> ..
  18. miRNAs and epidermal langerhans cell development and function
    Qing Sheng Mi; Fiscal Year: 2011
    ....
  19. REGULATION OF OVARIAN FUNCTION BY GONADOTROPIN
    JAIRAM K M MENON; Fiscal Year: 2013
    ..The studies proposed here address novel questions that are central to reproductive endocrinology and the results might provide new insights into novel approaches for the treatment of infertility. ..
  20. Elucidating the role of the eIF2alpha kinase PERK in synaptic plasticity and memo
    MY AI TRINH; Fiscal Year: 2010
    ....
  21. Role and Function of RNA-Binding Protein La in Oral Tumor Progression
    Gunhild Sommer; Fiscal Year: 2012
    ....
  22. Degradation of Endoplasmic Reticulum Chaperones by Chaperone-mediated Autophagy
    Roberta Kiffin; Fiscal Year: 2010
    ..Among these organelles, the endoplasmic reticulum (ER) plays a central role in protein biosynthesis and undergoes dramatic changes to accommodate the cellular demands of particular proteins...
  23. Single Molecule Dynamics of mRNA Translation
    Yale E Goldman; Fiscal Year: 2013
    ..Overall, our program of studying several pause ele- ments at the full protein expression level and within individual elongation cycles will define the magnitudes and detailed mechanisms of translation regulation. ..
  24. Regulated biosynthesis and function of ABC-transport systems
    Patrick H Thibodeau; Fiscal Year: 2013
    ..More than 1400 mutations have been identified in the CFTR, resulting in alterations to protein biosynthesis, trafficking, function, regulation and recycling...
  25. MicroRNA-Mediated Translation Initiation Arrest In Ischemic Brain
    Julie Anne Saugstad; Fiscal Year: 2013
    ..Our findings could also lead to development of novel stroke therapies based on miRNAs and their potential for regulating translation initiation in ischemic brain. ..
  26. Muscle protein synthesis and gene expression in myostatin-deficient mice
    Stephen L Welle; Fiscal Year: 2010
    ..Knowledge of the physiological and molecular effects of reducing myostatin activity in mature muscle will reveal potential benefits and limitations of anti-myostatin therapy. ..
  27. Analysis of Coronavirus-Host Cell Interactions
    Shinji Makino; Fiscal Year: 2013
    ....
  28. Hyperglycemia-induced translational control of gene expression in the retina
    Michael D Dennis; Fiscal Year: 2013
    ..abstract_text> ..
  29. MOLECULAR STUDIES OF RETINAL DEGENERATION IN DROSOPHILA
    Nansi J Colley; Fiscal Year: 2013
    ..Our research is focused on those events during protein biosynthesis in the secretory pathway that ensure correct protein translocation, glycosylation, folding, oligomeric ..
  30. Translational Regulation of Patterning in Drosophila
    Elizabeth R Gavis; Fiscal Year: 2013
    ..The proposed work will provide fundamental insight into how these factors control development, growth and differentiation, and how the disruption of translational control can result in disease. ..
  31. Nutritional Regulation of Leptin Production
    Susan K Fried; Fiscal Year: 2010
    ....
  32. TRANSMEMBRANE SIGNAL MODULATION BY HYPERTHERMIA
    STUART KEITH CALDERWOOD; Fiscal Year: 2013
    ....
  33. Regulation of maternal mRNA translation during early development
    EMRE UTKU SELI; Fiscal Year: 2013
    ..As a result of this enhanced understanding, development of targeted therapeutic strategies can ensue. ..
  34. Cap binding and gene expression in trypanosomes
    David A Campbell; Fiscal Year: 2013
    ..The UCLA group has an NIH award (AI056034;7/01/09-6/30/14) to study the maturation of the kinetoplastid spliced leader RNA. The goals of this FIRCA application are not related directly to the parent grant. ..
  35. New Models to Assay Gene Silencing Therapies
    Ronald J Mandel; Fiscal Year: 2012
    ....
  36. CARDIOVIRAL PROTEASES AND COMPARATIVE GENOME STRUCTURE
    Ann C Palmenberg; Fiscal Year: 2013
    ..These objectives build directly upon experimental foundations developed during the preceding 27 years of the program. ..
  37. Activity-dependent microRNA expression and function in the mature nervous system
    Karl H Obrietan; Fiscal Year: 2013
    ..These data should provide a framework to begin to develop therapeutic approaches designed to regulate microRNA expression. ..
  38. Vertebrate Non-Coding RNPs: Integrating function, biogenesis and survival
    Joan A Steitz; Fiscal Year: 2013
    ..Together, this understanding will contribute to the development of therapeutic approaches to combat multiple genetic diseases that derive from faulty RNA processing and surveillance, as well as cancer. ..
  39. Location: Its role in protein-RNA contact during trypanosome gene regulation
    Vivian Bellofatto; Fiscal Year: 2013
    ..brucei. This exploratory, two-year project is grounded in preliminary data, in a recent publication from our laboratory, preliminary data and complementary in vivo molecular genetic and in vitro biochemical approaches. ..
  40. Suppression of MMP-13 Expression in Arthritis by Pomegranate
    Tariq M Haqqi; Fiscal Year: 2013
    ..By studying the regulation of miRNA expression by bioavailable dietary constituents, our findings are likely to open the door for the development of novel therapeutic strategies for the treatment of OA and other diseases. ..
  41. The translational program in oocyte and early embryo development
    Marco Conti; Fiscal Year: 2013
    ....
  42. mRNA synthesis and capping in nonsegmented negative strand RNA viruses
    Tomoaki Ogino; Fiscal Year: 2013
    ..Furthermore, information derived from our research has the potential to guide future development of new anti-NNS RNA viral agents that specifically inhibit cap-forming activities of these L proteins. ..
  43. Molecular Analysis and Role of RGS6 as a Novel Growth Suppressor
    Rory A Fisher; Fiscal Year: 2010
    ..This work will determine how RGS6 works and will determine the basis for loss of RGS6 expression in human cancers. These studies could contribute to novel approaches in cancer therapy. ..
  44. Translational control during fetal male germ cell development
    CHRIS BENNETT GEYER; Fiscal Year: 2012
    ....
  45. BIOSYNTHESIS OF MEMBRANE PROTEIN GLYCOLIPID ANCHORS
    Anant K Menon; Fiscal Year: 2010
    ..We are interested in two critically important steps of GPI-protein biosynthesis: the attachment of a GPI anchor to protein and the enigmatic flip-flop of GPI lipids across the endoplasmic ..
  46. Role of microRNAs and RISC in activity regulated translation in neurons
    Gary J Bassell; Fiscal Year: 2013
    ..These studies are envisioned to have important implications for future therapeutic strategies to manipulate activity-regulated protein synthesis in the treatment of neurological disorders and drug addiction. ..
  47. The Gastric Biology of Helicobacter Pylori
    George Sachs; Fiscal Year: 2012
    ..5 in the niche of the colonizing organisms. Cell division, wall and protein biosynthesis genes were also increased...
  48. Virus and cell regulation of reovirus escape from translation inhibition
    Cathy L Miller; Fiscal Year: 2010
    ....
  49. Translational control of regulatory T cell induction
    Alexander Khoruts; Fiscal Year: 2010
    ..We are starting a novel mechanistic investigation of how regulatory T cells get induced by looking specifically at the role of protein translation control in the cells. ..
  50. Structural Aspects of Protein Synthesis in the Mammalian Mitochondria
    Rajendra K Agrawal; Fiscal Year: 2013
    ..which interact with a number of protein factors and RNA molecules to carry out the essential process of protein biosynthesis, or translation, in all cells, and also within organelles, such as mitochondria, in eukaryotic cells...
  51. Regulation of Neonatal Muscle Protein Synthesis
    Teresa A Davis; Fiscal Year: 2012
    ....
  52. Discovering small molecules activators of stress responsive signaling
    Jeffery W Kelly; Fiscal Year: 2013
    ..SUMMARY (See inslrucUons): The maintenance of protein homeostasis, or proteostasis, involves balancing protein biosynthesis, folding, vesicular trafficking, degradation, etc., which we hypothesize is critical for healthy aging...
  53. The effects of the unfolded protein response on medulloblastoma
    Wensheng Lin; Fiscal Year: 2013
    ..Activation of the pancreatic endoplasmic reticulum kinase (PERK) branch of the UPR inhibits global protein biosynthesis, but stimulates the expression of certain stress-induced genes, such as vascular endothelial growth factor ..
  54. Enterovirus RNA Translation and Replication
    David Barton; Fiscal Year: 2009
    ..These insights will be important for designing safe and effective vaccines for PV and other group C enteroviruses as well as antiviral drug therapies for many positive-strand RNA viruses. ..
  55. Role of mRNA translation in the effects of dietary restriction on lifespan
    Pankaj Kapahi; Fiscal Year: 2013
    ..Together this makes D. melanogaster an ideal system to examine the functional significance of tissue-specific changes on healthspan and aging. ..
  56. RiboTag: A novel technique to profile cell type specific gene expression and inv
    GEORGE STANLEY MCKNIGHT; Fiscal Year: 2013
    ..abstract_text> ..
  57. Translational Regulation of Mosquito mRNAs during Plasmodium Infection
    Jinsong Zhu; Fiscal Year: 2010
    ..The new insights in turn may lead to new strategies for malaria control. ..
  58. mTOR in Growth and Protection of Hypertrophying Myocardium
    Dhandapani Kuppuswamy; Fiscal Year: 2012
    ..These studies are envisioned to open new avenues of research into how the mTOR complexes influence hypertrophic growth and identify novel cardio-therapeutic strategies for rapamycin. ..
  59. Sensing Protein Folding Capacity in the Cell During Aging
    ROCKLAND L WISEMAN; Fiscal Year: 2010
    ..Proteostasis balances protein biosynthesis, folding, translocation, assembly/disassembly and clearance with the challenges imposed by environmental or ..
  60. Incorporating microRNA data and analyses into the leading cancer genomics portal
    Daniel R Rhodes; Fiscal Year: 2012
    ..If successful, this effort will improve public health by providing researchers with additional data and tools to understand and treat this biologically complex disease. ..
  61. Synaptic regulation of mTOR-dependent protein synthesis at synapses
    PATRICIA SALGADO; Fiscal Year: 2013
    ..These studies may also serve to inform subsequent analyses where physiological stimulation may be used to activate mTOR-dependent protein synthesis in cortical motor neurons to enable regeneration of axons after spinal cord injury. ..
  62. Alcohol Consumption and Risk of NHL: Role of MTOR Dysfunction
    Ronald B Gartenhaus; Fiscal Year: 2013
    ..abstract_text> ..
  63. CONTROL OF CELL FUNCTION VIA SELECTIVE MRNA TRANSLATION
    Lee Gehrke; Fiscal Year: 2002
    ..RNA-protein complexes have essential enzymatic and structural roles in many biological processes, including protein biosynthesis, virus replication, and virus assembly...
  64. NUCLEIC ACID PROBES OF RIBOSOMAL STRUCTURE AND FUNCTION
    BARRY COOPERMAN; Fiscal Year: 2003
    The ribosome is the unique site of protein biosynthesis in all cells, and as such a detailed understanding of its structure and function is of fundamental importance to the more general understanding of cellular function at the molecular ..
  65. Amino Acids and Muscle Protein Synthesis
    Robert Wolfe; Fiscal Year: 2006
    ..abstract_text> ..
  66. From Unfolded Proteins in the ER to Disease
    Linda Hendershot; Fiscal Year: 2009
    ..This conference will focus on recent advances in our understanding of the complexities of protein biosynthesis, folding, degradation, and cellular responses to the accumulation of misfolded proteins in the early ..
  67. Mechanisms of Response to GnRH Receptor Signaling
    MARK LAWSON; Fiscal Year: 2005
    ..Isolation of actively translated and total mRNA from treated and control cells will allow identification and quantification of global changes in gene expression. ..
  68. FACTORS WHICH RECOGNIZE MAMMALIAN POLYADENYLATION SIGNAL
    Claire Moore; Fiscal Year: 1993
    ..This will be studied in vivo using transient expression assays, and if possible, with an in vitro system capable of transcription initiation and elongation, polyadenylation, and termination...
  69. SHIGA TOXIN EFFECTS ON GENE REGULATION
    CHELESTE THORPE; Fiscal Year: 2003
    ..Through coursework, seminars, and supervised research, Dr. Thorpe will gain knowledge and expertise in techniques pertinent to the proposed research. ..
  70. CONTRACTILE PROTEIN ACCUMULATION IN AIRWAY SMOOTH MUSCLE
    Julian Solway; Fiscal Year: 2002
    ..abstract_text> ..