post transcriptional rna processing

Summary

Summary: Post-transcriptional biological modification of messenger, transfer, or ribosomal RNAs or their precursors. It includes cleavage, methylation, thiolation, isopentenylation, pseudouridine formation, conformational changes, and association with ribosomal protein.

Top Publications

  1. ncbi The Microprocessor complex mediates the genesis of microRNAs
    Richard I Gregory
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 432:235-40. 2004
  2. pmc The Drosha-DGCR8 complex in primary microRNA processing
    Jinju Han
    School of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151 742, Korea
    Genes Dev 18:3016-27. 2004
  3. ncbi A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA
    G Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Science 293:834-8. 2001
  4. ncbi A feedback loop comprising lin-28 and let-7 controls pre-let-7 maturation during neural stem-cell commitment
    Agnieszka Rybak
    Institute of Cell and Neurobiology, Center for Anatomy, Charite Universitatsmedizin Berlin, Berlin, Germany
    Nat Cell Biol 10:987-93. 2008
  5. ncbi The nuclear RNase III Drosha initiates microRNA processing
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    Nature 425:415-9. 2003
  6. pmc Selective blockade of microRNA processing by Lin28
    Srinivas R Viswanathan
    Stem Cell Program, Children s Hospital Boston, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA 02115, USA
    Science 320:97-100. 2008
  7. ncbi MicroRNAs: small RNAs with a big role in gene regulation
    Lin He
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Rev Genet 5:522-31. 2004
  8. ncbi RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals
    P D Zamore
    Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01655, USA
    Cell 101:25-33. 2000
  9. ncbi Processing of primary microRNAs by the Microprocessor complex
    Ahmet M Denli
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 432:231-5. 2004
  10. pmc Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs
    Xuezhong Cai
    Box 3025, Duke University Medical Center, Durham, NC 27710, USA
    RNA 10:1957-66. 2004

Research Grants

  1. Structural Studies of RNA Maturation and Processing
    Christopher D Lima; Fiscal Year: 2013
  2. Genomic analysis of RNA binding protein target specificity
    JEREMY ROBERT SANFORD; Fiscal Year: 2010
  3. RNA SPLICING IN ARCHAEA
    Ramesh Gupta; Fiscal Year: 2012
  4. RNA caps and meiotic pre-mRNA splicing
    Beate Schwer; Fiscal Year: 2013
  5. NUCLEIC ACIDS AND NEUCLEOPROTEINS
    ERNEST GEIDUSCHEK; Fiscal Year: 2007
  6. BRANCHED SUGAR NUCLEOSIDES AS ANTICANCER AGENTS
    Andre Rosowsky; Fiscal Year: 1980
  7. Proteins that Bind RNA
    ROBERT KRUG; Fiscal Year: 2001
  8. POSTTRANSCRIPTIONAL GENE REGULATION IN T CELL ACTIVATION
    JACK KEENE; Fiscal Year: 2004
  9. Regulation of Lung Epithelial Fibrinolysis by Urokinase
    Sreerama Shetty; Fiscal Year: 2005
  10. CONTROL OF FIBRINOLYTIC PATHWAYS IN LUNG DISEASE
    Sreerama Shetty; Fiscal Year: 2004

Detail Information

Publications295 found, 100 shown here

  1. ncbi The Microprocessor complex mediates the genesis of microRNAs
    Richard I Gregory
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 432:235-40. 2004
    ..In vivo knock-down and in vitro reconstitution studies revealed that both components of this smaller complex, termed Microprocessor, are necessary and sufficient in mediating the genesis of miRNAs from the primary miRNA transcript...
  2. pmc The Drosha-DGCR8 complex in primary microRNA processing
    Jinju Han
    School of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151 742, Korea
    Genes Dev 18:3016-27. 2004
    ..Based on these results, we propose a model for the action mechanism of class II RNase III proteins...
  3. ncbi A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA
    G Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Science 293:834-8. 2001
    ..Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression...
  4. ncbi A feedback loop comprising lin-28 and let-7 controls pre-let-7 maturation during neural stem-cell commitment
    Agnieszka Rybak
    Institute of Cell and Neurobiology, Center for Anatomy, Charite Universitatsmedizin Berlin, Berlin, Germany
    Nat Cell Biol 10:987-93. 2008
    ....
  5. ncbi The nuclear RNase III Drosha initiates microRNA processing
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    Nature 425:415-9. 2003
    ..Thus, the two RNase III proteins, Drosha and Dicer, may collaborate in the stepwise processing of miRNAs, and have key roles in miRNA-mediated gene regulation in processes such as development and differentiation...
  6. pmc Selective blockade of microRNA processing by Lin28
    Srinivas R Viswanathan
    Stem Cell Program, Children s Hospital Boston, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA 02115, USA
    Science 320:97-100. 2008
    ..Our results identify Lin28 as a negative regulator of miRNA biogenesis and suggest that Lin28 may play a central role in blocking miRNA-mediated differentiation in stem cells and in certain cancers...
  7. ncbi MicroRNAs: small RNAs with a big role in gene regulation
    Lin He
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Rev Genet 5:522-31. 2004
  8. ncbi RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals
    P D Zamore
    Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01655, USA
    Cell 101:25-33. 2000
    ..Cleavage occurs at sites 21-23 nucleotides apart, the same interval observed for the dsRNA itself, suggesting that the 21-23 nucleotide fragments from the dsRNA are guiding mRNA cleavage...
  9. ncbi Processing of primary microRNAs by the Microprocessor complex
    Ahmet M Denli
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 432:231-5. 2004
    ..Considered together, these results indicate a role for Pasha in miRNA maturation and miRNA-mediated gene regulation...
  10. pmc Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs
    Xuezhong Cai
    Box 3025, Duke University Medical Center, Durham, NC 27710, USA
    RNA 10:1957-66. 2004
    ..Together, these data show that human pri-miRNAs are not only structurally similar to mRNAs but can, in fact, function both as pri-miRNAs and mRNAs...
  11. ncbi A PUF family portrait: 3'UTR regulation as a way of life
    Marvin Wickens
    Dept of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Trends Genet 18:150-7. 2002
    ..Here, we discuss the evolution, biological function and mechanisms of action of the PUF protein family, and suggest that a primordial function of PUF proteins is to sustain mitotic proliferation of stem cells...
  12. pmc MicroRNA maturation: stepwise processing and subcellular localization
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Science, Seoul National University, Seoul 151 742, Korea
    EMBO J 21:4663-70. 2002
    ..Our study suggests that the regulation of miRNA expression may occur at multiple levels, including the two processing steps and the nuclear export step. These data will provide a framework for further studies on miRNA biogenesis...
  13. pmc SMAD proteins control DROSHA-mediated microRNA maturation
    Brandi N Davis
    Department of Biochemistry, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Nature 454:56-61. 2008
    ....
  14. ncbi Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA
    Inha Heo
    National Creative Research Center and School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    Mol Cell 32:276-84. 2008
    ..The Lin28-mediated downregulation of let-7 may play a key role in development, stem cell programming, and tumorigenesis...
  15. ncbi Pre-18S ribosomal RNA is structurally compacted into the SSU processome prior to being cleaved from nascent transcripts in Saccharomyces cerevisiae
    Yvonne N Osheim
    Department of Microbiology, University of Virginia Health System, Charlottesville, VA 22908, USA
    Mol Cell 16:943-54. 2004
    ..The results show that the approximately 40 components of the SSU processome/90S preribosome can complete their tasks within approximately 85 s in optimal conditions...
  16. ncbi From birth to death: the complex lives of eukaryotic mRNAs
    Melissa J Moore
    Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, 415 South Street, Waltham, MA 02454
    Science 309:1514-8. 2005
    ..Thus, a comprehensive understanding of eukaryotic gene expression requires an appreciation for how the lives of mRNAs are influenced by a wide array of diverse regulatory mechanisms...
  17. ncbi Human 5' --> 3' exonuclease Xrn2 promotes transcription termination at co-transcriptional cleavage sites
    Steven West
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Nature 432:522-5. 2004
    ..Degradation of the downstream cleavage product by Xrn2 results in transcriptional termination, as envisaged in the torpedo model...
  18. ncbi The C-terminal domain of RNA polymerase II couples mRNA processing to transcription
    S McCracken
    Amgen Institute, Toronto, Ontario, Canada
    Nature 385:357-61. 1997
    ....
  19. pmc Processing of intronic microRNAs
    Young Kook Kim
    Department of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Korea
    EMBO J 26:775-83. 2007
    ..Our study provides a novel example of eukaryotic gene organization and RNA-processing control...
  20. ncbi Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions
    Yoshiho Ikeuchi
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Mol Cell 21:97-108. 2006
    ....
  21. pmc Modulation of microRNA processing and expression through RNA editing by ADAR deaminases
    Weidong Yang
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nat Struct Mol Biol 13:13-21. 2006
    ..Consequently, mature miRNA-142 expression levels increased substantially in ADAR1 null or ADAR2 null mice. Our results demonstrate a new function of RNA editing in the control of miRNA biogenesis...
  22. ncbi CARPEL FACTORY, a Dicer homolog, and HEN1, a novel protein, act in microRNA metabolism in Arabidopsis thaliana
    Wonkeun Park
    Waksman Institute, Rutgers University, Piscataway, NJ 08854, USA
    Curr Biol 12:1484-95. 2002
    ....
  23. ncbi Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex
    Jinju Han
    School of Biological Sciences and Research Center for Functional Cellulomics, Seoul National University, Seoul
    Cell 125:887-901. 2006
    ..Our current study thus facilitates the prediction of novel microRNAs and will assist in the rational design of small hairpin RNAs for RNA interference...
  24. pmc The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb
    Brian D Harfe
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USA
    Proc Natl Acad Sci U S A 102:10898-903. 2005
    ..Thus, Dicer is required for the formation of normal mouse limbs. Strikingly, however, we did not detect defects in basic patterning or in tissue-specific differentiation of Dicer-deficient limb buds...
  25. ncbi RNA editing of AMPA receptor subunit GluR-B: a base-paired intron-exon structure determines position and efficiency
    M Higuchi
    Center for Molecular Biology, University of Heidelberg, Federal Republic of Germany
    Cell 75:1361-70. 1993
    ..Base conversion in the channel-coding region of GluR-B directed by base paired sequences may be executed by a ubiquitous nuclear adenosine deaminase specific for double-stranded RNA...
  26. ncbi Mechanisms of gene silencing by double-stranded RNA
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
    Nature 431:343-9. 2004
    ..These short dsRNAs guide RNA silencing by specific and distinct mechanisms. Many components of the RNA silencing machinery still need to be identified and characterized, but a more complete understanding of the process is imminent...
  27. ncbi Single processing center models for human Dicer and bacterial RNase III
    Haidi Zhang
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, 4002 Basel, Switzerland
    Cell 118:57-68. 2004
    ..Based on these and other data, we propose that Dicer functions through intramolecular dimerization of its two RNase III domains, assisted by the flanking RNA binding domains, PAZ and dsRBD...
  28. pmc Both RNase E and RNase III control the stability of sodB mRNA upon translational inhibition by the small regulatory RNA RyhB
    Taras Afonyushkin
    Max F Perutz Laboratories, Department of Microbiology and Immunobiology, University Departments at the Vienna Biocenter Dr Bohrgasse 9 4, A 1030 Vienna, Austria
    Nucleic Acids Res 33:1678-89. 2005
    ..These data are discussed in terms of a model, which accounts for the observed roles of RNase E and RNase III in sodB mRNA turnover...
  29. pmc Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast
    Andre P Gerber
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA
    PLoS Biol 2:E79. 2004
    ....
  30. pmc RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components
    Jennifer E G Gallagher
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520 8024, USA
    Genes Dev 18:2506-17. 2004
    ..This association may thus represent an early step in the formation of the SSU processome. Our results indicate that rRNA transcription and pre-rRNA processing are coordinated via specific components of the SSU processome...
  31. ncbi MicroRNAS and their regulatory roles in plants
    Matthew W Jones-Rhoades
    Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, and Howard Hughes Medical Institute, Cambridge, Massachusetts 02142, USA
    Annu Rev Plant Biol 57:19-53. 2006
    ....
  32. ncbi An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA
    Akiko Soma
    Department of Life Science, College of Science, Rikkyo St Paul s University, 3 34 1 Nishi Ikebukuro, Toshima ku, Tokyo 171 8501, Japan
    Mol Cell 12:689-98. 2003
    ..Partial inactivation of tilS in vivo resulted in an AUA codon-dependent translational defect, which supports the notion that TilS is an RNA-modifying enzyme that plays a critical role in the accurate decoding of genetic information...
  33. pmc Precision and functional specificity in mRNA decay
    Yulei Wang
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Proc Natl Acad Sci U S A 99:5860-5. 2002
    ..The results provide strong evidence that precise control of the decay of each mRNA is a fundamental feature of the gene expression program in yeast...
  34. ncbi RNA regulons: coordination of post-transcriptional events
    Jack D Keene
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Box 3020, Durham, North Carolina 27710, USA
    Nat Rev Genet 8:533-43. 2007
    ..I close by considering the evolutionary wiring and rewiring of these combinatorial post-transcriptional gene-expression networks...
  35. pmc RNase G (CafA protein) and RNase E are both required for the 5' maturation of 16S ribosomal RNA
    Z Li
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, FL 33101, USA
    EMBO J 18:2878-85. 1999
    ..These data indicate that both RNase E and CafA protein are required for a two step, sequential maturation of the 5' end of 16S rRNA, and that CafA protein is a new ribonuclease. We propose that it be renamed RNase G...
  36. ncbi Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance
    P Mourrain
    Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Versailles, France
    Cell 101:533-42. 2000
    ..Both sgs2 and sgs3 mutants show enhanced susceptibility to virus, definitively proving that PTGS is an antiviral defense mechanism that can also target transgene RNA for degradation...
  37. pmc RNA quality control: degradation of defective transfer RNA
    Zhongwei Li
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, FL 33101 6129, USA
    EMBO J 21:1132-8. 2002
    ..These data indicate that quality control of stable RNA metabolism in many ways resembles normal turnover of unstable RNA...
  38. pmc Widespread A-to-I RNA editing of Alu-containing mRNAs in the human transcriptome
    Alekos Athanasiadis
    Department of Biological Sciences, Lehigh University Bethlehem, Pennsylvania, USA
    PLoS Biol 2:e391. 2004
    ..The analysis suggests that modification of repetitive elements is a predominant activity for RNA editing with significant implications for cellular gene expression...
  39. ncbi Increased efficiency of mRNA 3' end formation: a new genetic mechanism contributing to hereditary thrombophilia
    N H Gehring
    Department of Pediatrics, , Humboldt University, Berlin, Germany
    Nat Genet 28:389-92. 2001
    ..This work also illustrates the pathophysiologic importance of quantitatively minor aberrations of RNA metabolism...
  40. pmc Functions of the exosome in rRNA, snoRNA and snRNA synthesis
    C Allmang
    Institute of Cell and Molecular Biology, University of Edinburgh, Swann Building, King s Buildings, Edinburgh EH9 3JR, UK
    EMBO J 18:5399-410. 1999
    ..We conclude that the exosome is involved in the processing of many RNA substrates and that different components can have distinct functions...
  41. ncbi The 7472insC mitochondrial DNA mutation impairs the synthesis and extent of aminoacylation of tRNASer(UCN) but not its structure or rate of turnover
    Marina Toompuu
    Institute of Medical Technology and Tampere University Hospital, FIN 33014 University of Tampere, Finland
    J Biol Chem 277:22240-50. 2002
    ..These findings indicate a common molecular process underlying sensorineural deafness caused by mitochondrial tRNA(Ser(UCN)) mutations...
  42. pmc Global analysis of Pub1p targets reveals a coordinate control of gene expression through modulation of binding and stability
    Radharani Duttagupta
    Department of Molecular Genetics, Microbiology, and Immunology, UMDNJ Robert Wood Johnson Medical School, 675 Hoes Ln, Piscataway, New Jersey 08854 5627, USA
    Mol Cell Biol 25:5499-513. 2005
    ..These results suggest that Pub1p binds to discrete subsets of cellular transcripts and posttranscriptionally regulates their expression at multiple levels...
  43. ncbi Human mitochondrial mRNAs are stabilized with polyadenylation regulated by mitochondria-specific poly(A) polymerase and polynucleotide phosphorylase
    Takashi Nagaike
    Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba Prefecture, Japan
    J Biol Chem 280:19721-7. 2005
    ..These results demonstrate that the poly(A) length of human mt mRNAs is controlled by polyadenylation by hmtPAP and deadenylation by hPNPase, and polyadenylation is required for the stability of mt mRNAs...
  44. ncbi Yeast Nfs1p is involved in thio-modification of both mitochondrial and cytoplasmic tRNAs
    Yumi Nakai
    Department of Biochemistry, Osaka Medical College, 2 7 Daigaku machi, Takatsuki, Osaka 569 8686, Japan
    J Biol Chem 279:12363-8. 2004
    ..These results suggest that Nfs1p is involved in the 2-thio-modification of both 5-carboxymethylaminomethyl-2-thiouridine in mt-tRNAs and mcm(5)s(2)U in cy-tRNAs...
  45. ncbi The human histone gene expression regulator HBP/SLBP is required for histone and DNA synthesis, cell cycle progression and cell proliferation in mitotic cells
    Xiujie Zhao
    School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK
    J Cell Sci 117:6043-51. 2004
    ..These findings indicate that human HBP/SLBP is essential for the coordinate synthesis of DNA and histone proteins and is required for progression through the cell division cycle...
  46. pmc Oncogenic All1 fusion proteins target Drosha-mediated microRNA processing
    Tatsuya Nakamura
    Department of Molecular Virology, Immunology, and Medical Genetics and Comprehensive Cancer Center, Ohio State University, Columbus, OH 43210
    Proc Natl Acad Sci U S A 104:10980-5. 2007
    ..Finally, we present evidence from ChIP experiments for All1 fusion protein-mediated recruitment of Drosha to target genes encoding miRNAs. Such recruitment may underlie the enhanced expression of the relevant miRNAs...
  47. ncbi A protein complex mediating mRNA degradation in Escherichia coli
    B Py
    Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, Oxford, UK
    Mol Microbiol 14:717-29. 1994
    ..The identification of a complex which includes several activities associated with mRNA degradation has implications for the mechanisms and co-ordinated control of mRNA degradation...
  48. pmc Ultraconserved elements are associated with homeostatic control of splicing regulators by alternative splicing and nonsense-mediated decay
    Julie Z Ni
    Center for Molecular Biology of RNA and Department of Molecular, Cell, and Developmental Biology, University of California at Santa Cruz, Santa Cruz, California 95064, USA
    Genes Dev 21:708-18. 2007
    ....
  49. ncbi Mechanisms of microRNA-mediated gene regulation in animal cells
    Timothy W Nilsen
    Center for RNA Molecular Biology, Case Western Reserve University, School of Medicine, W127, 10900 Euclid Avenue Cleveland, OH 44106 4973, USA
    Trends Genet 23:243-9. 2007
    ..In this review, I describe the evidence that there are multiple mechanisms of microRNA-mediated repression and discuss the possible connections between these mechanisms...
  50. pmc Heterogeneity in polyadenylation cleavage sites in mammalian mRNA sequences: implications for SAGE analysis
    E Pauws
    Laboratory of Pediatric Endocrinology and Bioinformatics Laboratory, Academic Medical Center, University of Amsterdam, PO Box 22700, 1100 DE Amsterdam, The Netherlands
    Nucleic Acids Res 29:1690-4. 2001
    ..Both experimental and in silico data show that the selection of the specific cleavage site for poly(A) addition using a given polyadenylation signal is more variable than was previously thought...
  51. pmc The nuclear exosome contributes to autogenous control of NAB2 mRNA levels
    Kelly M Roth
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Box 672, 601 Elmwood Ave, Rochester, NY 14642, USA
    Mol Cell Biol 25:1577-85. 2005
    ..These findings suggest a novel regulatory circuit in which the nuclear exosome controls the level of NAB2 mRNA in response to changes in the activity of Nab2 protein...
  52. pmc Extensive post-transcriptional regulation of microRNAs and its implications for cancer
    J Michael Thomson
    Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Genes Dev 20:2202-7. 2006
    ..These data uncover a novel regulatory step in miRNA function and provide a mechanism for miRNA down-regulation in cancer...
  53. pmc Bop1 is a mouse WD40 repeat nucleolar protein involved in 28S and 5. 8S RRNA processing and 60S ribosome biogenesis
    Z Strezoska
    Department of Molecular Genetics, University of Illinois at Chicago College of Medicine, Chicago, Illinois 60607 7170, USA
    Mol Cell Biol 20:5516-28. 2000
    ..These studies thus identify Bop1 as a novel, nonribosomal mammalian protein that plays a key role in the formation of the mature 28S and 5.8S rRNAs and in the biogenesis of the 60S ribosomal subunit...
  54. pmc LIN-28 and the poly(U) polymerase PUP-2 regulate let-7 microRNA processing in Caenorhabditis elegans
    Nicolas J Lehrbach
    Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, The Henry Wellcome Building of Cancer and Developmental Biology, Cambridge, UK
    Nat Struct Mol Biol 16:1016-20. 2009
    ..Uridylation by a PUP-2 ortholog might regulate let-7 and additional miRNAs in other species. Given the roles of Lin28 and let-7 in stem cell and cancer biology, we propose that such poly(U) polymerases are potential therapeutic targets...
  55. pmc Identification of the gene encoding the 5S ribosomal RNA maturase in Bacillus subtilis: mature 5S rRNA is dispensable for ribosome function
    C Condon
    Unité Propre de Recherche 9073, Institut de Biologie Physico Chimique, Paris, France
    RNA 7:242-53. 2001
    ..A potential correlation between the presence of RNase E or RNase M5 and the single- or double-stranded nature of the predicted cleavage sites is explored...
  56. ncbi Single nucleotide polymorphism associated with mature miR-125a alters the processing of pri-miRNA
    Ranhui Duan
    Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA
    Hum Mol Genet 16:1124-31. 2007
    ..Thus, our study reveals an additional structural requirement for pri-miRNA processing and emphasizes the importance of identifying new miRNA SNPs and their contributions to miRNA biogenesis and human genetic disease...
  57. pmc The Saccharomyces cerevisiae RNase mitochondrial RNA processing is critical for cell cycle progression at the end of mitosis
    Ti Cai
    Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, 50 E Adams Street, Syracuse, NY 13210, USA
    Genetics 161:1029-42. 2002
    ..These results indicate a clear role of RNase MRP in cell cycle progression at the end of mitosis. Conservation of this function in humans may explain many of the pleiotropic phenotypes of cartilage hair hypoplasia...
  58. pmc Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATP
    Haidi Zhang
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, CH 4002 Basel, Switzerland
    EMBO J 21:5875-85. 2002
    ..Additional experiments suggest that if ATP participates in the Dicer reaction in mammalian cells, it might be involved in product release needed for the multiple turnover of the enzyme...
  59. ncbi Pseudouridine synthases
    Tomoko Hamma
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, Washington 98109, USA
    Chem Biol 13:1125-35. 2006
    ....
  60. pmc A tRNA-like structure is present in 10Sa RNA, a small stable RNA from Escherichia coli
    Y Komine
    Department of Biophysics, Faculty of Science, Kyoto University, Japan
    Proc Natl Acad Sci U S A 91:9223-7. 1994
    ..A pair could be overcome by the introduction of a gene encoding alanyl-tRNA synthetase (alaS) on a multicopy plasmid. The evidence suggests that the proposed structural features of 10Sa RNA are indeed manifested in vivo...
  61. pmc Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation
    Johannes H Urban
    Max Planck Institute for Infection Biology, RNA Biology Group, Berlin, Germany
    PLoS Biol 6:e64. 2008
    ..Our study reveals hierarchical action of two well-conserved sRNAs in a complex regulatory cascade that controls the glmS mRNA. Similar cascades of noncoding RNA regulators may operate in other organisms...
  62. ncbi tRNA transfers to the limelight
    Anita K Hopper
    Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Genes Dev 17:162-80. 2003
  63. pmc The N-terminal double-stranded RNA binding domains of Arabidopsis HYPONASTIC LEAVES1 are sufficient for pre-microRNA processing
    Feijie Wu
    National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
    Plant Cell 19:914-25. 2007
    ..We suggest that the N-terminal dsRBDs fulfill the function of the whole HYL1 and thus play an essential role in miRNA processing and miRNA-directed silencing of targeted genes...
  64. pmc Processing of the precursors to small nucleolar RNAs and rRNAs requires common components
    E Petfalski
    Institute of Cell and Molecular Biology, University of Edinburgh, United Kingdom
    Mol Cell Biol 18:1181-9. 1998
    ..8S rRNA. Processing of the pre-rRNA and both intronic and polycistronic snoRNAs therefore involves common components...
  65. ncbi A conserved small RNA promotes discoordinate expression of the glmUS operon mRNA to activate GlmS synthesis
    Johannes H Urban
    Max Planck Institute for Infection Biology, RNA Biology, Chariteplatz 1, 10117 Berlin, Germany
    J Mol Biol 373:521-8. 2007
    ..Our data suggest that Gram-negative bacteria evolved a mechanism of glmS riboregulation that is distinct from the glmS riboswitch mechanism of Gram-positive bacteria...
  66. pmc Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes
    Tiffany D Miles
    Department of Biological Sciences, Carnegie Mellon University, 616 Mellon Institute, 4400 Fifth Ave, Pittsburgh, PA 15213, USA
    Mol Cell Biol 25:10419-32. 2005
    ..Mutations in the WD40 motifs of Ytm1 disrupt binding to Erb1, destabilize the heterotrimer, and delay pre-rRNA processing and nuclear export of preribosomes. Nevertheless, 66S preribosomes lacking Ytm1 remain otherwise intact...
  67. ncbi Cold shock induction of RNase R and its role in the maturation of the quality control mediator SsrA/tmRNA
    Fatima Cairrao
    Instituto Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apt 127, 2781 901 Oeiras, Portugal
    Mol Microbiol 50:1349-60. 2003
    ..The wide biological significance of RNase R regarding adaptation to cold shock and its involvement in RNA surveillance, protein quality control and pathogenesis is discussed...
  68. ncbi Ribonuclease E provides substrates for ribonuclease P-dependent processing of a polycistronic mRNA
    P Alifano
    Dipartimento di Biologia e Patologia Cellulare e Molecolare L Califano, Universita Degli Studi Di Napoli, Italy
    Genes Dev 8:3021-31. 1994
    ..This is the first evidence that ribonuclease P, an essential ribozyme required for the biosynthesis of tRNA, may also be involved in the segmental stabilization of a mRNA...
  69. ncbi Dissecting Arabidopsis thaliana DICER function in small RNA processing, gene silencing and DNA methylation patterning
    Ian R Henderson
    Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, California 90095, USA
    Nat Genet 38:721-5. 2006
    ..Our studies also suggest that asymmetric DNA methylation can be maintained by pathways that do not require siRNAs...
  70. pmc Sequence requirements for micro RNA processing and function in human cells
    Yan Zeng
    Howard Hughes Medical Institute, Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 9:112-23. 2003
    ..These results suggest that miRNAs, and the closely similar small interfering RNAs, cannot totally discriminate between RNA targets differing by a single nucleotide...
  71. ncbi Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs
    Jürgen Soutschek
    Alnylam Europe AG, Fritz Hornschuch Str 9, 95326 Kulmbach, Germany
    Nature 432:173-8. 2004
    ..These findings demonstrate the therapeutic potential of siRNAs for the treatment of disease...
  72. ncbi Identification of modified residues in RNAs by reverse transcription-based methods
    Yuri Motorin
    Laboratoire de Maturation des ARN et Enzymologie Moleculaire, Faculte des Sciences et Techniques, Nancy Universite, Vandouevre les Nancy, France
    Methods Enzymol 425:21-53. 2007
    ..Advice for an optimized use of these methods and for correct interpretation of the data is also given. We also provide some helpful information on the ability of other naturally occurring modified nucleotides to generate RT pauses...
  73. pmc The putative NTPase Fap7 mediates cytoplasmic 20S pre-rRNA processing through a direct interaction with Rps14
    Sander Granneman
    Yale University School of Medicine, Department of Molecular Biophysics and Biochemistry, 333 Cedar Street, SHM C 114, New Haven, CT 06520 8024, USA
    Mol Cell Biol 25:10352-64. 2005
    ..We propose that Fap7 mediates cleavage of the 20S pre-rRNA at site D by directly interacting with Rps14 and speculate that it is an enzyme that functions as an NTP-dependent molecular switch in 18S rRNA maturation...
  74. pmc RNase MRP cleaves the CLB2 mRNA to promote cell cycle progression: novel method of mRNA degradation
    Tina Gill
    Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
    Mol Cell Biol 24:945-53. 2004
    ..In addition, these results denote a new mechanism of mRNA degradation not seen before in the yeast Saccharomyces cerevisiae...
  75. pmc Stress granules and processing bodies are dynamically linked sites of mRNP remodeling
    Nancy Kedersha
    Division of Rheumatology and Immunology, Harvard Medical School, Brigham and Women s Hospital, Boston, MA 02115, USA
    J Cell Biol 169:871-84. 2005
    ..We propose that mRNA released from disassembled polysomes is sorted and remodeled at SGs, from which selected transcripts are delivered to PBs for degradation...
  76. pmc RNA helicase Prp43 and its co-factor Pfa1 promote 20 to 18 S rRNA processing catalyzed by the endonuclease Nob1
    Brigitte Pertschy
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    J Biol Chem 284:35079-91. 2009
    ..In ltv1/pfa1 mutants the 20 S pre-rRNA was susceptible to 3' to 5' degradation by the cytoplasmic exosome. This degraded into the 3' region of the 18 S rRNA, strongly indicating that the preribosomes are structurally defective...
  77. pmc Nip7p interacts with Nop8p, an essential nucleolar protein required for 60S ribosome biogenesis, and the exosome subunit Rrp43p
    N I Zanchin
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    Mol Cell Biol 19:1518-25. 1999
    ..Distinct pools of Rrp43p may interact both with the exosome and with Nip7p, possibly both in the nucleus and in the cytoplasm, to catalyze analogous reactions in the multistep process of 60S ribosome biogenesis and mRNA turnover...
  78. pmc A plant snoRNP complex containing snoRNAs, fibrillarin, and nucleolin-like proteins is competent for both rRNA gene binding and pre-rRNA processing in vitro
    Julio Saez-Vasquez
    Laboratoire Génome et Développement des Plantes, UMR CNRS IRD 5096, Universite de Perpignan, France
    Mol Cell Biol 24:7284-97. 2004
    ..Based on this it is proposed that NF D is a processing complex that assembles on the rDNA prior to its interaction with the nascent pre-rRNA...
  79. pmc Rrp17p is a eukaryotic exonuclease required for 5' end processing of Pre-60S ribosomal RNA
    Marlene Oeffinger
    Rockefeller University, New York, NY 10065, USA
    Mol Cell 36:768-81. 2009
    ..We show that Rrp17p binds late pre-60S ribosomes, accompanying them from the nucleolus to the nuclear periphery, and provide evidence for physical and functional links between late 60S subunit processing and export...
  80. ncbi High-definition macromolecular composition of yeast RNA-processing complexes
    Nevan J Krogan
    Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario M5G 1L6, Canada
    Mol Cell 13:225-39. 2004
    ..This study provides a high-resolution overview of the modular topology of noncoding RNA-processing machinery...
  81. ncbi A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis
    François Dragon
    Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520 8040, USA
    Nature 417:967-70. 2002
    ..We have termed this large RNP the small subunit (SSU) processome...
  82. ncbi Homologs of small nucleolar RNAs in Archaea
    A D Omer
    Department of Biochemistry and Molecular Biology, University of British Columbia, 2146 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada
    Science 288:517-22. 2000
    ..snoRNA-based rRNA processing was therefore probably present in the last common ancestor of Archaea and Eukarya, predating the evolution of a morphologically distinct nucleolus...
  83. ncbi Cold-temperature induction of Escherichia coli polynucleotide phosphorylase occurs by reversal of its autoregulation
    R K Beran
    1602 Molecular Science, Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA
    Mol Microbiol 39:112-25. 2001
    ..Together with other evidence, these results suggest that mRNA is generally stabilized upon a shift to cold temperatures, but that a CSR mRNA-specific decay process is initiated during adaptation...
  84. ncbi The stability of tristetraprolin mRNA is regulated by mitogen-activated protein kinase p38 and by tristetraprolin itself
    Carmen R Tchen
    Kennedy Institute of Rheumatology Division, Imperial College London, 1 Aspenlea Road, Hammersmith, London W6 8LH, United Kingdom
    J Biol Chem 279:32393-400. 2004
    ..We also demonstrate that TTP is able to bind to its own 3'-untranslated region and negatively regulate its own expression, forming a feedback loop to limit expression levels...
  85. ncbi P bodies: at the crossroads of post-transcriptional pathways
    Ana Eulalio
    Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    Nat Rev Mol Cell Biol 8:9-22. 2007
    ....
  86. ncbi Arabidopsis HEN1: a genetic link between endogenous miRNA controlling development and siRNA controlling transgene silencing and virus resistance
    Stéphanie Boutet
    Laboratoire de Biologie Cellulaire, INRA, 78026 Versailles Cedex, France
    Curr Biol 13:843-8. 2003
    ....
  87. pmc Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1
    Irina Chernyakov
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642, USA
    Genes Dev 22:1369-80. 2008
    ..The RTD pathway is the first to be implicated in the turnover of mature RNA species from the class of stable RNAs. These results and the results of others demonstrate that tRNA, like mRNA, is subject to multiple quality control steps...
  88. ncbi RNA surveillance. Unforeseen consequences for gene expression, inherited genetic disorders and cancer
    M R Culbertson
    Laboratory of Genetics, R M Bock Laboratories, University of Wisconsin, Madison 53706, USA
    Trends Genet 15:74-80. 1999
    ..The NMD pathway has a direct impact on hundreds of genetic disorders in the human population, where about a quarter of all known mutations are predicted to trigger NMD...
  89. pmc Degradation of RNA in bacteria: comparison of mRNA and stable RNA
    Murray P Deutscher
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, PO Box 016129, Miami, FL 33101 6129, USA
    Nucleic Acids Res 34:659-66. 2006
    ..Rather, only the specificity of the RNase and the accessibility of the substrate determine whether or not a particular RNA will be acted upon...
  90. ncbi Kinetics of the processing of the precursor to 4.5 S RNA, a naturally occurring substrate for RNase P from Escherichia coli
    K A Peck-Miller
    Department of Biology, Yale University, New Haven, CT 06520
    J Mol Biol 221:1-5. 1991
    ..5S, is cleaved with greater efficiency than the parent molecule. The 3'-terminal CCC sequence of p4.5 S may be as important for cleavage of this substrate as the 3'-terminal CCA sequence is for cleavage of tRNA precursors...
  91. ncbi SnoRNP biogenesis meets Pre-mRNA splicing
    Tamas Kiss
    Laboratoire de Biologie Moleculaire Eucaryote du CNRS, 118 route de Narbonne, 31062 Toulouse, France
    Mol Cell 23:775-6. 2006
    ..In the September 1 issue of Molecular Cell, Hirose et al. (2006) demonstrate that a spliceosomal intron binding protein, IBP160, couples box C/D snoRNA processing with pre-mRNA splicing in the C1 splicing complex...
  92. pmc A human, ATP-independent, RISC assembly machine fueled by pre-miRNA
    Elisavet Maniataki
    Department of Pathology, Division of Neuropathology, University of Pennsylvania School of Medicine, Philadelphia, 19104, USA
    Genes Dev 19:2979-90. 2005
    ..We suggest that the miRLC couples the processing of the pre-miRNA substrate to the unwinding of the product and that after loading of the mature miRNA to Ago2, the miRLC disassembles and the miRNP is released...
  93. pmc Maturation of 23S ribosomal RNA requires the exoribonuclease RNase T
    Z Li
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101, USA
    RNA 5:139-46. 1999
    ..coli, is able to efficiently remove nucleotides close to the double-stranded stem generated by the pairing of the 5' and 3' termini of most stable RNAs...
  94. pmc A general two-metal-ion mechanism for catalytic RNA
    T A Steitz
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511
    Proc Natl Acad Sci U S A 90:6498-502. 1993
    ..The role of the RNA is to position the two catalytic metal ions and properly orient the substrates via three specific binding sites...
  95. pmc Defining the enzyme binding domain of a ribonuclease III processing signal. Ethylation interference and hydroxyl radical footprinting using catalytically inactive RNase III mutants
    H Li
    Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA
    EMBO J 15:1421-33. 1996
    ..1[WC-L] RNA, and dsRNA in general, while catalyzing only single cleavage of R1.1 RNA and related substrates in which the scissle bond is within an asymmetric internal loop...
  96. pmc Role and dynamics of the ribosomal protein P0 and its related trans-acting factor Mrt4 during ribosome assembly in Saccharomyces cerevisiae
    María Rodríguez-Mateos
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas and Universidad Autonoma de Madrid, Cantoblanco E 28049 Madrid, Spain
    Nucleic Acids Res 37:7519-32. 2009
    ..We conclude that Mrt4 controls the position and timing of P0 assembly. In turn, P0 is required for the release of Mrt4 and exchanges with this factor at the cytoplasm. Our results also suggest other P0 assembly alternatives...
  97. ncbi RNase PH is essential for tRNA processing and viability in RNase-deficient Escherichia coli cells
    K O Kelly
    Department of Biochemistry, University of Connecticut Health Center, Farmington 06030 3305
    J Biol Chem 267:16015-8. 1992
    ..These data indicate that RNase PH can be an essential enzyme for the processing of tRNA precursors...
  98. pmc Poly(A) tail length control in Saccharomyces cerevisiae occurs by message-specific deadenylation
    C E Brown
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
    Mol Cell Biol 18:6548-59. 1998
    ..The ability to control the length of the poly(A) tail for newly expressed mRNAs has the potential to be an important posttranscriptional regulatory step in gene expression...
  99. ncbi Principles and effects of microRNA-mediated post-transcriptional gene regulation
    B M Engels
    Division of Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, UK
    Oncogene 25:6163-9. 2006
    ....
  100. ncbi The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases
    P Mitchell
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 91:457-66. 1997
    ..8S rRNA. Human Rrp4p is found in a comparably sized complex, and expression of the hRRP4 gene in yeast complements the rrp4-1 mutation. We conclude that the exosome constitutes a highly conserved eukaryotic RNA processing complex...
  101. ncbi A stepwise model for double-stranded RNA processing by ribonuclease III
    Jianhua Gan
    Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA
    Mol Microbiol 67:143-54. 2008
    ..Together, the crystal structures and the models suggest a stepwise mechanism for RNase III to execute the phosphoryl transfer reaction...

Research Grants70

  1. Structural Studies of RNA Maturation and Processing
    Christopher D Lima; Fiscal Year: 2013
    ..Pathways and enzymes that regulate the abundance, lifetime, and processing of RNA have been associated with human diseases that include neurodegenerative disorders, cancer, and inflammation. ..
  2. Genomic analysis of RNA binding protein target specificity
    JEREMY ROBERT SANFORD; Fiscal Year: 2010
    ..2) Determine the genomic landscape of cis-acting RNA elements recognized by SR proteins. (3) Functionally validate SR protein cis-acting RNA elements. ..
  3. RNA SPLICING IN ARCHAEA
    Ramesh Gupta; Fiscal Year: 2012
    ..Thus, the overall understanding of these processes in archaeal systems will help us understand similar processes in human systems under normal conditions, and the changes that may occur under diseased conditions. ..
  4. RNA caps and meiotic pre-mRNA splicing
    Beate Schwer; Fiscal Year: 2013
    ..We expect to gain new and general insights to the question of how splice site choice can be modulated by components of the basal splicing machinery in response to distinct features in the pre-mRNA. ! ..
  5. NUCLEIC ACIDS AND NEUCLEOPROTEINS
    ERNEST GEIDUSCHEK; Fiscal Year: 2007
    ....
  6. BRANCHED SUGAR NUCLEOSIDES AS ANTICANCER AGENTS
    Andre Rosowsky; Fiscal Year: 1980
    ....
  7. Proteins that Bind RNA
    ROBERT KRUG; Fiscal Year: 2001
    ....
  8. POSTTRANSCRIPTIONAL GENE REGULATION IN T CELL ACTIVATION
    JACK KEENE; Fiscal Year: 2004
    ..In summary, HuR will be examined for RNA recognition specificity and interactions, which govern its effects on the stability or translation of cytokine and ERG mRNAs during T cell activation. ..
  9. Regulation of Lung Epithelial Fibrinolysis by Urokinase
    Sreerama Shetty; Fiscal Year: 2005
    ..This information could hasten the development of new, mechanism-based interventions for lung disorders, including acute lung injury or lung neoplasia. ..
  10. CONTROL OF FIBRINOLYTIC PATHWAYS IN LUNG DISEASE
    Sreerama Shetty; Fiscal Year: 2004
    ..abstract_text> ..
  11. Aminoacyl-tRNA synthetases in Archaea
    Ya Ming Hou; Fiscal Year: 2006
    ..jannaschii to gain a broader insight into its aminoacyl-tRNA synthetases as a basis for understanding the origin and evolution of contemporary mRNA-encoded protein synthesis in extant organisms. [unreadable] [unreadable]..
  12. Post-transcriptional regulation of TNF-alpha production
    Paul Anderson; Fiscal Year: 2005
    ..These studies might also identify molecular targets for the development of orally available TNFa blockers that can be used to treat patients with rheumatoid arthritis. ..
  13. RNA Transport Within the Nucleus
    Thoru Pederson; Fiscal Year: 2005
    ..Another aspect of the project will address the intranuclear traffic of these RNAs in relation to proteins known to be involved in nuclear import or RNA export, i.e. the Ran-GTPase and TAP. ..
  14. Biogenesis of Eukaryotic Ribosomes
    SUSAN GERBI; Fiscal Year: 2004
    ..The proposed studies should elucidate the importance of dynamic RNA-RNA interactions during ribosome biogenesis, a fundamental process essential for cell growth and viability. ..
  15. Molecular Mechanism of HIV-1 Vif Function
    BRYAN CULLEN; Fiscal Year: 2007
    ..Together, this wide-ranging series of experiments should lead to considerable insight into how HIV-1 Vif functions and potentially suggest approaches to inhibit Vif function. ..
  16. Maintenance of the tRNA 3' end
    Ya Ming Hou; Fiscal Year: 2008
    ..Because of the importance of CCA in maintenance of cellular life, the proposed studies will have a strong impact on human health and diseases. ..
  17. Regulation of human microRNA biosynthesis and activity
    BRYAN CULLEN; Fiscal Year: 2007
    ..Finally, in specific aim 5, we seek to extend our exciting initial data demonstrating inhibition of miRNA function by the adenovirus VA1 non-coding RNA by trying to define the underlying mechanism. ..
  18. Dynamic Regulation of y-Globin mRNA Stability
    J Russell; Fiscal Year: 2004
    ....
  19. Effect of APOBEC3G on retroviruses and retrotransposons
    BRYAN CULLEN; Fiscal Year: 2009
    ..abstract_text> ..
  20. RNA PROCESSING AND RIBONUCLEOPROTEIN
    Thoru Pederson; Fiscal Year: 2001
    ..The same studies should provide information on sequences required for nuclear export. These two inter-related projects test the hypothesis that the nucleolus is a more pluri-functional organelle than previously thought. ..
  21. Gene Amplification-Sciarid DNA Puffs
    SUSAN GERBI; Fiscal Year: 2006
    ..Information gained from the unique Sciara system should behelpful in understanding the mechanisms used by metazoa to specify an origin and to regulate its activation. ..
  22. HIV-1 TROPISM AND CHEMOKINE RECEPTORS
    BRYAN CULLEN; Fiscal Year: 2002
    ..The final specific aim focusses on the promoter regions of CCR5, the identification of transcription factors that may interact with the promoter, and the use of antisense strategies to block CCR5 expression. ..
  23. Characterization of mammalian stress granules
    Paul J Anderson; Fiscal Year: 2010
    ..These studies have implications for our understanding of stress-regulated pathological conditions, including Alzheimer's disease, diabetes and cancer. ..
  24. REGULATION OF GENE EXPRESSION BY NO/CGMP
    RENATE PILZ; Fiscal Year: 2002
    ....
  25. ROLE OF PSGL 1 IN NATURAL KILLER CELL FUNCTION
    Paul Anderson; Fiscal Year: 2003
    ..Consequently, the overall goal of the proposed experiments is to determine how PEN5:L-selectin interactions influence NK cell function. ..
  26. The role of FAST in immune-mediated inflammatory disease
    Paul Anderson; Fiscal Year: 2008
    ..Finally, we will generate transgenic mice that overexpress FAST and mutant mice that lack FAST to determine its importance in the initiation and/or propagation of immune-mediated inflammatory disease. ..
  27. FUNCTIONAL ANALYSIS OF AN RNA STRUCTURAL MOTIF
    Ya Ming Hou; Fiscal Year: 2005
    ..coli CysRS for its ability to distinguish cysteine from serine. These studies shall shed new light on the molecular interactions responsible for the accurate translation of the genetic code. ..
  28. NUTRITIONAL COPPER STATUS AND THE NERVOUS SYSTEM
    JOSEPH ROBERT PROHASKA; Fiscal Year: 2010
    ..AIM 4: We will test the hypothesis that limitation in Cu-dependent ferroxidases lead to lower brain iron and are responsible by comparing rats reared on an iron-fortified diet to restore brain iron. ..
  29. Role of PKR in Growth Regulation
    Glen Barber; Fiscal Year: 2008
    ..We propose that these aims will lend insight into mechanisms of cell signaling, tumorigenesis and interferon-mediated actions in the cell. ..
  30. Targeted pre-mRNA modification and gene silencing
    Yi Tao Yu; Fiscal Year: 2007
    ..We believe that our novel method will prove effective and useful in mammalian systems as well as in yeast. [unreadable] [unreadable] [unreadable]..
  31. A Study of Site-Specifically Modified RNAs
    Christine Chow; Fiscal Year: 2006
    ..abstract_text> ..
  32. Analyzing respiration-dependent control of cell fate in S. cerevisiae
    Ashwini Jambhekar; Fiscal Year: 2007
    ..This work will define how cells choose between growth and gametogenesis. [unreadable] [unreadable] [unreadable]..
  33. The Functional Analysis of the Coactivator CARM1
    Mark Bedford; Fiscal Year: 2007
    ..abstract_text> ..
  34. Complement mediated lysis resistance genes of Leishmania
    JEFFREY BEETHAM; Fiscal Year: 2008
    ..abstract_text> ..
  35. A Nucleosomal Biosensor for Identification and Isolation of Nuclear Hormone Recep
    Richard Steinman; Fiscal Year: 2008
    ..This biosensor should be more rapid and versatile than cell-based reporter gene assays and more informative than assays that measure only ligand/receptor binding. [unreadable] [unreadable] [unreadable]..
  36. GenMAPP-CS, a dynamic resource pathway analysis
    Bruce R Conklin; Fiscal Year: 2010
    ..The challenge of analyzing these massive and complex datasets is a major force driving the development of GenMAPP-CS. ..
  37. NICOTINE EFFECTS ON THE ADRENAL MEDULLA AND BRAIN
    ARNOLD TANK; Fiscal Year: 2009
    ..3) To test whether PCBP and the polyC-rich sequence to which it binds in the 3'UTR of TH mRNA participate in the stabilization of TH mRNA in response to the activation of signaling pathways that mediate the response to nicotine. ..
  38. Understanding the Mechanism of RNA Interference (RNAi)
    Phillip Zamore; Fiscal Year: 2009
    ....
  39. microRNA Uncoupling of Protein and Transcript Expression in Liver Regeneration
    CLIFFORD JOHN STEER; Fiscal Year: 2010
    ..Ultimately, the results may provide the basis for novel therapies designed to promote liver regeneration and accelerate recovery from disease states. ..
  40. Regulation of translation initiation in eukaryotes
    Vincent P Mauro; Fiscal Year: 2010
    ..g. for the production of therapeutic proteins, and new avenues for analyzing the potential contributions of mis-regulation to disease. ..
  41. Comparative Evolutionary Genomics and Infectious Disease
    Jane Carlton; Fiscal Year: 2010
    ....
  42. Molecular Mechanisms of Portal Hypertension
    Vijay Shah; Fiscal Year: 2007
    ..abstract_text> ..
  43. In Vitro Reconstitution of Alpha Fetroprotein Regulation
    Michelle Barton; Fiscal Year: 2007
    ..To test this hypothesis, we will assemble AFP gene templates into developmentally repressed, solid-phase nuclei and analyze chromatin structure and transcription function before and after DNA replication. ..
  44. Genetic Analysis of Heavy Metals Neurotoxicity
    Fen Biao Gao; Fiscal Year: 2005
    ....
  45. Regulation of Expression of Secreted Flt-1
    WILLIAM HUCKLE; Fiscal Year: 2005
    ..abstract_text> ..
  46. Gordon Research Conference on Myogenesis
    STEPHEN TAPSCOTT; Fiscal Year: 2004
    ..abstract_text> ..
  47. The Role of mRNA Editing in B Cell Development
    Harold Smith; Fiscal Year: 2004
    ..abstract_text> ..
  48. RECONSTITUTION OF GENE REGULATION IN LIVER REGENERATION
    Michelle Barton; Fiscal Year: 2003
    ..The long-term goal of these studies is to learn how regenerative organs, such as the liver are capable of redirecting their genetic programs in response to traumatic injury. ..
  49. IRON TRANSPORT IN MAMMALIAN CELLS
    Philip Aisen; Fiscal Year: 2003
    ..Project 5: Structure, function and dynamics in transferrin investigates the molecular mechanisms through which the uptake and release of iron and other metals by transferrin are modulated by physiological factors. ..
  50. REG OF NITRIC OXIDE PRODUCT IN THE HEPATIC CIRCULATION
    Vijay Shah; Fiscal Year: 2002
    ..Defining the mechanisms that regulate nitric oxide production in SEC may lead to therapeutic options whereby NO activity may be pharmacologically or genetically modulated and benefit patients with cirrhosis and portal hypertension. ..
  51. NEUROD AND NEUROGENESIS
    STEPHEN TAPSCOTT; Fiscal Year: 2001
    ..Th health relatedness of this work is that the ability to generate neurons and neuroendocrine cells will be critical for the treatment of numerous human diseases. ..
  52. Attenuation of mucous cell metaplasia by Rad
    Mary Chang; Fiscal Year: 2006
    ..In summary, we have proposed a comprehensive approach utilizing combined in vitro, cell, and in vivo methods to test our hypothesis. ..
  53. The Role of DGAT1 In Muscle Lipotoxicity
    Yi Hao Yu; Fiscal Year: 2006
    ..As a result, we may be able to identify new molecular target(s) for future drug therapy aimed at reducing lipotoxicity ..
  54. MAP kinase signal specificity in the C. elegans germline
    Valerie Reinke; Fiscal Year: 2007
    ..Together these experiments should build toward a comprehensive understanding of the mechanisms used to produce a specific cell fate in response to a generally used signaling pathway. ..
  55. EXPRESSION AND STRUCTURE OF THE CALCIUM SENSING RECEPTOR
    Rajiv Kumar; Fiscal Year: 2007
    ..Furthermore, this information will serve as the basis for more extensive experiments involving the structure of the full-length receptor. [unreadable] [unreadable]..
  56. Control of Spemann's organizer
    Michael Sheets; Fiscal Year: 2007
    ..We will address our view by examining the role(s) of FGF signaling pathway(s) within the organizer and the transcription of chordin as they relate to the ultimate inducing functions of Spemann's organizer. [unreadable] [unreadable]..
  57. Small Molecule Regulators of Arginine Methyltransferases
    Mark Bedford; Fiscal Year: 2007
    ..Most importantly, the PRMTs are a newly identified family of enzymes that have not yet been tapped as "drugable" targets. ..
  58. Isotopically Labeled Nucleotides for Biomolecular NMR
    James Williamson; Fiscal Year: 2007
    ..In order to make these reagents widely available to the structural biology community, we propose to optimize and scale up production of these reagents for commercial distribution. ..
  59. IRON BINDING PROTEINS AND CONTROL OF IRON METABOLISM
    Philip Aisen; Fiscal Year: 2001
    ..Understanding the fundamental chemistry of iron transport, storage and utilization will provide the rational basis for managing the multitude of diseases where aberrations of iron metabolism contribute to cellular injury and death. ..
  60. Genetic and Biochemical Analysis of SID-1 and SID-2
    Craig Hunter; Fiscal Year: 2007
    ..Although RNAi has been shown to be effective, a major obstacle remains delivery of dsRNA into human cells both in culture and in vivo. ..
  61. Assemply of the 30S Ribosomal Subunit
    James Williamson; Fiscal Year: 2007
    ..This will allow us to monitor the 30S assembly from a global perspective. [unreadable] [unreadable]..
  62. MOLECULAR MECHANISMS OF PML MEDIATED GROWTH CONTROL
    KATHERINE BORDEN; Fiscal Year: 2005
    ....
  63. Molecul Biology of Intestinal Lipid Transport/Metabolism
    NICHOLAS DAVIDSON; Fiscal Year: 2003
    ..abstract_text> ..
  64. Nucleic Acid Gordon Research Conference
    ANNA PYLE; Fiscal Year: 2004
    ..Poster sessions and optional recreational activities will stimulate informal interactions among participants. ..
  65. Immunogenicity of Hepatitis C Virus (HCV) like particles
    Glen Barber; Fiscal Year: 2007
    ..abstract_text> ..
  66. REGULATIOIN OF RENAL PHOSPHATE EXCRETION AND VITAMIN D METABOLISM BY FGF 7
    Rajiv Kumar; Fiscal Year: 2010
    ..The interaction between the FGF 7 and other PTNs will be clarified. Such information will significantly enhance our knowledge of mineralization processes and Pi homeostasis. ..
  67. Innate Intracellular Mechanisms
    Glen N Barber; Fiscal Year: 2010
    ..The importance of STING in innate signaling will be determined. Understanding how these pathways function has significant impact on understanding pathogenesis and for developing novel therapeutics and vaccines to combat disease. ..
  68. TH GENE REGULATION IN HEALTHY AND LESIONED MIDBRAIN
    ARNOLD TANK; Fiscal Year: 2003
    ..These studies will shed light on the molecular mechanisms regulating the TH gene in the midbrain and may lead to new therapeutic strategies for the treatment of Parkinson's disease. ..
  69. Receptor-Signaling Protein Interactions in Real Time
    Sidney Pestka; Fiscal Year: 2007
    ..Further application of this technology should lead to new rapid methods for high throughput screening and for identifying protein-protein interactions in live cells in real-time. ..