fungal gene expression regulation

Summary

Summary: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.

Top Publications

  1. pmc Genomic expression programs in the response of yeast cells to environmental changes
    A P Gasch
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5428, USA
    Mol Biol Cell 11:4241-57. 2000
  2. pmc Bidirectional promoters generate pervasive transcription in yeast
    Zhenyu Xu
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nature 457:1033-7. 2009
  3. pmc High-resolution DNA-binding specificity analysis of yeast transcription factors
    Cong Zhu
    Division of Genetics, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Genome Res 19:556-66. 2009
  4. ncbi The economics of ribosome biosynthesis in yeast
    J R Warner
    Dept of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, New York, Bronx, NY 10461, USA
    Trends Biochem Sci 24:437-40. 1999
  5. ncbi Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus
    William C Nierman
    The Institute for Genomic Research, Rockville, Maryland 20850, USA
    Nature 438:1151-6. 2005
  6. pmc A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors
    Hildur V Colot
    Department of Genetics, Dartmouth Medical School, HB7400, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 103:10352-7. 2006
  7. pmc Extension of chronological life span in yeast by decreased TOR pathway signaling
    R Wilson Powers
    Department of Genome Sciences and Medicine, The Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195, USA
    Genes Dev 20:174-84. 2006
  8. pmc Global transcriptional responses of fission yeast to environmental stress
    Dongrong Chen
    The Wellcome Trust Sanger Institute, Cambridge CB10 1SA, United Kingdom
    Mol Biol Cell 14:214-29. 2003
  9. ncbi Systematic identification of pathways that couple cell growth and division in yeast
    Paul Jorgensen
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8
    Science 297:395-400. 2002
  10. pmc Strains and strategies for large-scale gene deletion studies of the diploid human fungal pathogen Candida albicans
    Suzanne M Noble
    Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94143 2200, USA
    Eukaryot Cell 4:298-309. 2005

Detail Information

Publications310 found, 100 shown here

  1. pmc Genomic expression programs in the response of yeast cells to environmental changes
    A P Gasch
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5428, USA
    Mol Biol Cell 11:4241-57. 2000
    ..Physiological themes in the genomic responses to specific environmental stresses provided insights into the effects of those stresses on the cell...
  2. pmc Bidirectional promoters generate pervasive transcription in yeast
    Zhenyu Xu
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nature 457:1033-7. 2009
    ....
  3. pmc High-resolution DNA-binding specificity analysis of yeast transcription factors
    Cong Zhu
    Division of Genetics, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Genome Res 19:556-66. 2009
    ..These approaches could be adapted to identify TFs and cis regulatory elements in higher eukaryotes...
  4. ncbi The economics of ribosome biosynthesis in yeast
    J R Warner
    Dept of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, New York, Bronx, NY 10461, USA
    Trends Biochem Sci 24:437-40. 1999
    ..This is entrusted to a number of signal transduction pathways that can abruptly induce or silence the ribosomal genes, leading to major implications for the expression of other genes as well...
  5. ncbi Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus
    William C Nierman
    The Institute for Genomic Research, Rockville, Maryland 20850, USA
    Nature 438:1151-6. 2005
    ..The Af293 genome sequence provides an unparalleled resource for the future understanding of this remarkable fungus...
  6. pmc A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors
    Hildur V Colot
    Department of Genetics, Dartmouth Medical School, HB7400, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 103:10352-7. 2006
    ..Overall, the methodology, which achieves high-throughput gene disruption at an efficiency >90% in this filamentous fungus, promises to be applicable to other eukaryotic organisms that have a low frequency of homologous recombination...
  7. pmc Extension of chronological life span in yeast by decreased TOR pathway signaling
    R Wilson Powers
    Department of Genome Sciences and Medicine, The Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195, USA
    Genes Dev 20:174-84. 2006
    ..We propose that up-regulation of a highly conserved response to starvation-induced stress is important for life span extension by decreased TOR signaling in yeast and higher eukaryotes...
  8. pmc Global transcriptional responses of fission yeast to environmental stress
    Dongrong Chen
    The Wellcome Trust Sanger Institute, Cambridge CB10 1SA, United Kingdom
    Mol Biol Cell 14:214-29. 2003
    ..Promoter motifs associated with some of the groups of coregulated genes were identified. We compare and contrast global regulation of stress genes in fission and budding yeasts and discuss evolutionary implications...
  9. ncbi Systematic identification of pathways that couple cell growth and division in yeast
    Paul Jorgensen
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8
    Science 297:395-400. 2002
    ..Cell growth and division appear to be coupled by multiple conserved mechanisms...
  10. pmc Strains and strategies for large-scale gene deletion studies of the diploid human fungal pathogen Candida albicans
    Suzanne M Noble
    Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94143 2200, USA
    Eukaryot Cell 4:298-309. 2005
    ..albicans strains. We demonstrate these procedures for two well-studied genes, TUP1 and EFG1, as well as a novel gene, RBD1. These tools should permit large-scale genetic analysis of this important human pathogen...
  11. pmc Structure and function of the feed-forward loop network motif
    S Mangan
    Departments of Molecular Cell Biology and Physics of Complex Systems, Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 100:11980-5. 2003
    ..Additional features, such as pulse generation and cooperativity, are discussed. This study defines the function of one of the most significant recurring circuit elements in transcription networks...
  12. pmc Environmental sensing and signal transduction pathways regulating morphopathogenic determinants of Candida albicans
    Subhrajit Biswas
    National Centre for Plant Genome Research, New Delhi 110 067, India
    Microbiol Mol Biol Rev 71:348-76. 2007
    ..The downstream components of these pathways, some of which may be interesting antifungal targets, are also discussed...
  13. pmc Biofilm matrix regulation by Candida albicans Zap1
    Clarissa J Nobile
    Department of Microbiology, Columbia University, New York, New York, United States of America
    PLoS Biol 7:e1000133. 2009
    ..Our findings define a novel regulatory circuit and its mechanism of control of a process central to infection...
  14. pmc LaeA, a regulator of secondary metabolism in Aspergillus spp
    Jin Woo Bok
    Department of Plant Pathology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Eukaryot Cell 3:527-35. 2004
    ....
  15. ncbi Logic of the yeast metabolic cycle: temporal compartmentalization of cellular processes
    Benjamin P Tu
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, L3 124, Dallas, TX 75390, USA
    Science 310:1152-8. 2005
    ..Essential cellular and metabolic events occur in synchrony with the metabolic cycle, demonstrating that key processes in a simple eukaryotic cell are compartmentalized in time...
  16. pmc Genetic interactions between polymorphisms that affect gene expression in yeast
    Rachel B Brem
    Program in Computational Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, M2 B876, Seattle, Washington 98109, USA
    Nature 436:701-3. 2005
    ..Our results indicate that genetic interactions are widespread in the genetics of transcript levels, and that many QTLs will be missed by single-locus tests but can be detected by two-stage tests that allow for interactions...
  17. ncbi Transcriptional induction of genes encoding endoplasmic reticulum resident proteins requires a transmembrane protein kinase
    J S Cox
    Department of Biochemistry and Biophysics, University of California Medical School, San Francisco 94143 0448
    Cell 73:1197-206. 1993
    ..IRE1 is also required for inositol prototrophy, suggesting that the induction of ER resident proteins is coupled to the biogenesis of new ER membrane...
  18. ncbi A suppressor of two essential checkpoint genes identifies a novel protein that negatively affects dNTP pools
    X Zhao
    Department of Genetics and Development, Columbia University, College of Physicians and Surgeons, New York, New York 10032 2704, USA
    Mol Cell 2:329-40. 1998
    ..Finally, both in vivo and in vitro experiments show that Sml1 binds to Rnr1. We propose that Sml1 inhibits dNTP synthesis posttranslationally by binding directly to Rnr1 and that Mec1 and Rad53 are required to relieve this inhibition...
  19. pmc Coordination of growth rate, cell cycle, stress response, and metabolic activity in yeast
    Matthew J Brauer
    Lewis Sigler Institute for Integrative Genomics and Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Mol Biol Cell 19:352-67. 2008
    ..This concept is useful in interpreting the system-level connections among growth rate, metabolism, stress, and the cell cycle...
  20. pmc Glucose signaling in Saccharomyces cerevisiae
    George M Santangelo
    Department of Biological Sciences, University of Southern Mississippi, Hattiesburg, MS 39406 5018, USA
    Microbiol Mol Biol Rev 70:253-82. 2006
    ..Completion of this task should result in a much improved understanding of eukaryotic growth, differentiation, and carcinogenesis...
  21. ncbi The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II
    Minkyu Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 432:517-22. 2004
    ..These findings support a model in which poly(A) site cleavage and subsequent degradation of the 3'-downstream RNA by Rat1 trigger transcription termination...
  22. ncbi Genetic dissection of transcriptional regulation in budding yeast
    Rachel B Brem
    Fred Hutchinson Cancer Research Center FHCRC, 1100 Fairview Avenue North, D4 100, Seattle, WA 98109, USA and Howard Hughes Medical Institute
    Science 296:752-5. 2002
    ..We found eight such trans-acting loci, each affecting the expression of a group of 7 to 94 genes of related function...
  23. ncbi Genome-wide localization of the nuclear transport machinery couples transcriptional status and nuclear organization
    Jason M Casolari
    Department of Systems Biology, Harvard Medical School and Department of Cancer Biology, The Dana Farber Cancer Institute, Boston, MA 02115, USA
    Cell 117:427-39. 2004
    ..Taken together, these results indicate that the organization of the genome is coupled via transcriptional state to the nuclear transport machinery...
  24. ncbi Dynamic repertoire of a eukaryotic transcriptome surveyed at single-nucleotide resolution
    Brian T Wilhelm
    Cancer Research UK Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK
    Nature 453:1239-43. 2008
    ..Splicing efficiency was largely coordinated with transcript levels, and increased transcription led to increased splicing in test genes. Hundreds of introns showed such regulated splicing during cellular proliferation or differentiation...
  25. ncbi Global control of dimorphism and virulence in fungi
    Julie C Nemecek
    Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, University of Wisconsin Hospital and Clinics, Madison, WI 53792, USA
    Science 312:583-8. 2006
    ..This global regulator shapes how dimorphic fungal pathogens adapt to the mammalian host, which has broad implications for treating and preventing systemic fungal disease...
  26. pmc High-resolution mapping of meiotic crossovers and non-crossovers in yeast
    Eugenio Mancera
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nature 454:479-85. 2008
    ..In addition, because non-crossover hotspots create holes of reduced linkage within haplotype blocks, our results stress the need to incorporate non-crossovers into genetic linkage analysis...
  27. pmc A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae
    V Guacci
    Howard Hughes Medical Institute, Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
    Cell 91:47-57. 1997
    ..These data implicate Mcd1p as a nexus between cohesion and condensation. We present a model for mitotic chromosome structure that incorporates this previously unsuspected link...
  28. ncbi ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe
    Akihisa Matsuyama
    Chemical Genetics Laboratory, RIKEN, Wako, Saitama 351 0198, Japan
    Nat Biotechnol 24:841-7. 2006
    ..Furthermore, using leptomycin B, an inhibitor of the nuclear export protein Crm1, we identified 285 proteins whose localization is regulated by Crm1...
  29. ncbi Gene duplication and the adaptive evolution of a classic genetic switch
    Chris Todd Hittinger
    Howard Hughes Medical Institute, Laboratory of Genetics, University of Wisconsin Madison, 1525 Linden Drive, Madison, Wisconsin 53706, USA
    Nature 449:677-81. 2007
    ....
  30. pmc LaeA, a regulator of morphogenetic fungal virulence factors
    Jin Woo Bok
    University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Eukaryot Cell 4:1574-82. 2005
    ..LaeA, a conserved protein in filamentous fungi, is a developmental regulator of virulence genes and, possibly, the first antimicrobial target specific to filamentous fungi that are pathogenic to plants and animals...
  31. pmc Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea
    N J Talbot
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 1392
    Plant Cell 5:1575-90. 1993
    ..Our results suggest that hydrophobins may have a role in the elaboration of infective structures by fungi and may fulfill other functions in fungal phytopathogenesis...
  32. ncbi The transcriptional program of meiosis and sporulation in fission yeast
    Juan Mata
    The Wellcome Trust Sanger Institute, Cambridge CB10 1SA, UK
    Nat Genet 32:143-7. 2002
    ....
  33. pmc Stress-activated genomic expression changes serve a preparative role for impending stress in yeast
    David B Berry
    Laboratory of Genetics and Genome Center of Wisconsin, University of Wisconsin, Madison, WI 53706, USA
    Mol Biol Cell 19:4580-7. 2008
    ..This work highlights the importance of condition-specific responses in acquired stress resistance and provides new insights into the role of the ESR...
  34. ncbi The transcriptional response of Saccharomyces cerevisiae to osmotic shock. Hot1p and Msn2p/Msn4p are required for the induction of subsets of high osmolarity glycerol pathway-dependent genes
    M Rep
    Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Kardinaal Mercierlaan 92, B 3001 Leuven Heverlee, Flanders, Belgium
    J Biol Chem 275:8290-300. 2000
    ..Inappropriate activation of the mating response may contribute to the growth defect of a hog1 mutant in high osmolarity medium...
  35. pmc Critical role of Bcr1-dependent adhesins in C. albicans biofilm formation in vitro and in vivo
    Clarissa J Nobile
    Department of Microbiology, Columbia University, New York, New York, USA
    PLoS Pathog 2:e63. 2006
    ..Known adhesins Als1 and Hwp1 also contribute to biofilm formation, as does the novel protein Ece1...
  36. ncbi Histone H3 methylation by Set2 directs deacetylation of coding regions by Rpd3S to suppress spurious intragenic transcription
    Michael J Carrozza
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Cell 123:581-92. 2005
    ..This erases transcription elongation-associated acetylation to suppress intragenic transcription initiation...
  37. ncbi Cln3 activates G1-specific transcription via phosphorylation of the SBF bound repressor Whi5
    Robertus A M de Bruin
    Department of Molecular Biology, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Cell 117:887-98. 2004
    ..Like mammalian Rb, Whi5 is a G1-specific transcriptional repressor antagonized by CDK...
  38. ncbi Transcription elongation factors repress transcription initiation from cryptic sites
    Craig D Kaplan
    Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Science 301:1096-9. 2003
    ..Other elongation and chromatin factors, including Spt16 and histone H3, appear to contribute to this control...
  39. ncbi Transcriptional regulation of biomass-degrading enzymes in the filamentous fungus Trichoderma reesei
    Pamela K Foreman
    Genencor International, Inc, Palo Alto, California 94304, USA
    J Biol Chem 278:31988-97. 2003
    ..However, genes whose products are involved in protein processing and secretion were not highly regulated during cellulase induction...
  40. ncbi PKA and Sch9 control a molecular switch important for the proper adaptation to nutrient availability
    Johnny Roosen
    Functional Biology, Katholieke Universiteit Leuven, Kasteelpark Arenberg, B 3001 Leuven Heverlee, Belgium
    Mol Microbiol 55:862-80. 2005
    ..This mechanism allows fine-tuning various nutritional responses in yeast cells, allowing them to adapt metabolism and growth appropriately...
  41. pmc Widespread selection for local RNA secondary structure in coding regions of bacterial genes
    Luba Katz
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genome Res 13:2042-51. 2003
    ..These results are interpreted in terms of possible roles of RNA structures in RNA processing, regulation of mRNA stability, and translational control...
  42. ncbi nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine
    K Maundrell
    Sclavo Research Centre, Siena, Italy
    J Biol Chem 265:10857-64. 1990
    ..The nmt1 promotor has been subcloned, and thiamine-mediated transcriptional control has been transferred to the bacterial reporter gene chloramphenicol acetyltransferase...
  43. pmc The landscape of genetic complexity across 5,700 gene expression traits in yeast
    Rachel B Brem
    Division of Human Biology and Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Proc Natl Acad Sci U S A 102:1572-7. 2005
    ..Sixteen percent of highly heritable transcripts exhibit evidence of interacting loci. Our results will aid design of future QTL mapping studies and may shed light on the evolution of quantitative traits...
  44. pmc Two strategies for gene regulation by promoter nucleosomes
    Itay Tirosh
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Genome Res 18:1084-91. 2008
    ..Analysis of nucleosome positioning in human promoters reproduces the main observations. Our results suggest two distinct strategies for gene regulation by chromatin, which are selectively employed by different genes...
  45. pmc Transcriptional regulation of chemical diversity in Aspergillus fumigatus by LaeA
    Robyn M Perrin
    Department of Plant Pathology, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS Pathog 3:e50. 2007
    ..Our findings suggest that LaeA is a novel target for comprehensive modification of chemical diversity and pathogenicity...
  46. ncbi Regulation of secondary metabolism in filamentous fungi
    Jae Hyuk Yu
    Department of Food Microbiology and Toxicology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Annu Rev Phytopathol 43:437-58. 2005
    ..Particularly, the roles of G protein signaling components and developmental regulators in the mycotoxin sterigmatocystin biosynthesis in the model fungus Aspergillus nidulans are discussed in depth...
  47. ncbi Nitrogen regulation in Saccharomyces cerevisiae
    Boris Magasanik
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Gene 290:1-18. 2002
    ..A second focus of the review is the nitrogen regulation of the general amino acid permease, Gap1p, and the proline permease, Put4p, by ubiquitin mediated intracellular protein sorting in the secretory and endosomal pathways...
  48. ncbi White-opaque switching in Candida albicans is controlled by mating-type locus homeodomain proteins and allows efficient mating
    Mathew G Miller
    Department of Microbiology and Immunology, University of California, San Francisco 94143, USA
    Cell 110:293-302. 2002
    ..As white cells are generally more robust in a mammalian host than are opaque cells, this strategy allows the organism to survive the rigors of life within a mammalian host, yet generate mating-competent cells...
  49. ncbi Large-scale essential gene identification in Candida albicans and applications to antifungal drug discovery
    Terry Roemer
    Elitra Canada, Montreal, Quebec, Canada, H2X 3Y8
    Mol Microbiol 50:167-81. 2003
    ..albicans essential gene set, and their respective conditional mutant strains may be directly used as sensitive whole-cell assays for drug screening...
  50. pmc Epigenetic properties of white-opaque switching in Candida albicans are based on a self-sustaining transcriptional feedback loop
    Rebecca E Zordan
    Department of Biochemistry and Biophysics, 600 16th Street, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 103:12807-12. 2006
    ..Thus, this simple model can account for both the heritability of the white and opaque states and the stochastic nature of the switching between them...
  51. ncbi Regulation of cell-surface genes and biofilm formation by the C. albicans transcription factor Bcr1p
    Clarissa J Nobile
    Department of Microbiology, Columbia University, New York, NY 10032, USA
    Curr Biol 15:1150-5. 2005
    ..Our results indicate that hyphal cells are specialized to present adherence molecules that support biofilm integrity...
  52. ncbi Fungal morphogenesis and host invasion
    Neil A R Gow
    Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, AB25 2ZD, Aberdeen, UK
    Curr Opin Microbiol 5:366-71. 2002
    ..This review considers the role of morphogenesis in fungal infection and argues that no simple, universal relationship can be drawn between morphology and the invasive potential of a fungus...
  53. pmc Arginine methylation at histone H3R2 controls deposition of H3K4 trimethylation
    Antonis Kirmizis
    Gurdon Institute and Department of Pathology, Tennis Court Road, Cambridge CB2 1QN, UK
    Nature 449:928-32. 2007
    ..Thus, the inability of Spp1 to recognize H3 methylated at R2 prevents Set1 from trimethylating H3K4. These results provide the first mechanistic insight into the function of arginine methylation on chromatin...
  54. ncbi Genetic and epigenetic regulation of the FLO gene family generates cell-surface variation in yeast
    Adrian Halme
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Cell 116:405-15. 2004
    ..These sources of epigenetic and genetic variation explain the observed heterogeneity of cell-surface protein expression within a population of cells derived from a single clone...
  55. pmc A conserved stress-activated protein kinase regulates a core stress response in the human pathogen Candida albicans
    Deborah A Smith
    School of Cell and Molecular Biosciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom
    Mol Biol Cell 15:4179-90. 2004
    ..This niche specificity is reflected by the specific environmental conditions that drive the Hog1-regulated core stress response in C. albicans and by differences in the molecular circuitry that control this response...
  56. ncbi Translational regulation of GCN4 and the general amino acid control of yeast
    Alan G Hinnebusch
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Annu Rev Microbiol 59:407-50. 2005
    ..Remarkably, mammalian cells use the same strategy to downregulate protein synthesis while inducing transcriptional activators of stress-response genes under various stressful conditions, including amino acid starvation...
  57. pmc Tetracycline-inducible gene expression and gene deletion in Candida albicans
    Yang Nim Park
    Institut für Molekulare Infektionsbiologie, Universitat Wurzburg, Rontgenring 11, D 97070 Wurzburg, Germany
    Eukaryot Cell 4:1328-42. 2005
    ..albicans and provides a convenient, versatile new tool to study gene function and manipulate cellular behavior in this model pathogenic fungus...
  58. pmc Bistable expression of WOR1, a master regulator of white-opaque switching in Candida albicans
    Guanghua Huang
    State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
    Proc Natl Acad Sci U S A 103:12813-8. 2006
    ..We present genetic evidence for feedback regulation of WOR1 transcription. The feedback regulation explains the bistable and stochastic nature of white-opaque switching...
  59. pmc Metabolism of sulfur amino acids in Saccharomyces cerevisiae
    D Thomas
    Centre de Genetique Moleculaire, CNRS, Gif sur Yvette, France
    Microbiol Mol Biol Rev 61:503-32. 1997
    ..The AdoMet-mediated control of the sulfur amino acid pathway illustrates the molecular strategies used by eucaryotic cells to couple gene expression to metabolic changes...
  60. pmc A fungal family of transcriptional regulators: the zinc cluster proteins
    Sarah MacPherson
    Department of Microbiology and Immunology, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada H3A 1A
    Microbiol Mol Biol Rev 70:583-604. 2006
    ..With the number of characterized zinc cluster proteins growing rapidly, it is becoming more and more apparent that they are important regulators of fungal physiology...
  61. pmc NRG1 represses yeast-hypha morphogenesis and hypha-specific gene expression in Candida albicans
    A M Murad
    Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, UK
    EMBO J 20:4742-52. 2001
    ..Hence, CaNrg1 is a transcriptional repressor that appears to target CaTup1 to a distinct set of virulence-related functions, including yeast-hypha morphogenesis...
  62. ncbi The veA gene activates sexual development in Aspergillus nidulans
    Hee Kim
    Basic Science Research Institute, Division of Biological Sciences, Chonbuk National University, Chonju, Chonbuk 561 756, Republic of Korea
    Fungal Genet Biol 37:72-80. 2002
    ..These results indicated that the veA gene is a positive regulator in sexual development and simultaneously a negative one in asexual development...
  63. ncbi Cohesins bind to preferential sites along yeast chromosome III, with differential regulation along arms versus the centric region
    Y Blat
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Cell 98:249-59. 1999
    ..The methodology described can be used to analyze the distribution of any DNA-binding protein and, via microchips, along entire genomes...
  64. ncbi CDK activity antagonizes Whi5, an inhibitor of G1/S transcription in yeast
    Michael Costanzo
    Department of Medical Genetics and Microbiology, University of Toronto, 1 King s College Circle, Toronto, M5S 1A8, Canada
    Cell 117:899-913. 2004
    ..Elimination of CDK activity at the end of mitosis allows Whi5 to reenter the nucleus to again repress G1/S transcription. These findings harmonize G1/S control in eukaryotes...
  65. pmc veA is required for toxin and sclerotial production in Aspergillus parasiticus
    Ana M Calvo
    Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA
    Appl Environ Microbiol 70:4733-9. 2004
    ....
  66. ncbi In vivo transcript profiling of Candida albicans identifies a gene essential for interepithelial dissemination
    Katherina Zakikhany
    Division Mycology, Robert Koch Institute, Berlin, Germany
    Cell Microbiol 9:2938-54. 2007
    ..Our results suggest that the adaptive response and morphology of C. albicans play specific roles for host-fungal interactions during mucosal infections...
  67. ncbi The glyoxylate cycle is required for fungal virulence
    M C Lorenz
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Massachusetts 02142, USA
    Nature 412:83-6. 2001
    ....
  68. ncbi Specificity and stability in topology of protein networks
    Sergei Maslov
    Department of Physics, Brookhaven National Laboratory, Upton, NY 11973, USA
    Science 296:910-3. 2002
    ..This effect decreases the likelihood of cross talk between different functional modules of the cell and increases the overall robustness of a network by localizing effects of deleterious perturbations...
  69. ncbi Flocculation, adhesion and biofilm formation in yeasts
    Kevin J Verstrepen
    Harvard University Bauer Center for Genomics Research, 7 Divinity Avenue, Room 104, Cambridge, MA 02138, USA
    Mol Microbiol 60:5-15. 2006
    ..These aspects of fungal adhesion exemplify the impressive phenotypic plasticity of yeasts, allowing them to adapt quickly to stressful environments and exploit new opportunities...
  70. pmc Genome-wide analysis of light-inducible responses reveals hierarchical light signalling in Neurospora
    Chen Hui Chen
    Department of Genetics, Dartmouth Medical School, Hanover, NH 03755 3844, USA
    EMBO J 28:1029-42. 2009
    ..The data provide a global picture of transcriptional response to light, as well as illuminating the cis- and trans-acting elements comprising the regulatory signalling cascade that governs the photobiological response...
  71. ncbi Exploring the metabolic and genetic control of gene expression on a genomic scale
    J L DeRisi
    Department of Biochemistry, Stanford University School of Medicine, Howard Hughes Medical Institute, Stanford, CA 94305 5428, USA
    Science 278:680-6. 1997
    ..These results demonstrate the feasibility and utility of this approach to genomewide exploration of gene expression patterns...
  72. ncbi Genome-wide analysis of mRNAs regulated by the nonsense-mediated and 5' to 3' mRNA decay pathways in yeast
    Feng He
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Mol Cell 12:1439-52. 2003
    ....
  73. pmc Hsp104, Hsp70 and Hsp40 interplay regulates formation, growth and elimination of Sup35 prions
    James Shorter
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Stellar Chance Laboratories, Philadelphia, PA 19104, USA
    EMBO J 27:2712-24. 2008
    ..This activity is reduced when Ssa1, or enhanced when Ssb1, is incorporated into nascent prions. These findings illuminate several facets of the chaperone interplay that underpins [PSI(+)] inheritance...
  74. ncbi TOR and PKA signaling pathways converge on the protein kinase Rim15 to control entry into G0
    Ivo Pedruzzi
    Department of Medical Biochemistry, CMU, University of Geneva, 1211 Geneva 4, Switzerland
    Mol Cell 12:1607-13. 2003
    ..Thus, Rim15 integrates signals from at least three nutrient-sensory kinases (TOR, PKA, and Sch9) to properly control entry into G(0), a key developmental process in eukaryotic cells...
  75. pmc Quantification of protein half-lives in the budding yeast proteome
    Archana Belle
    Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, 94143 2240, USA
    Proc Natl Acad Sci U S A 103:13004-9. 2006
    ..Our experimental data and theoretical analysis underscore the importance of an integrative approach to the complex interplay between protein degradation, transcriptional regulation, and other determinants of protein metabolism...
  76. ncbi Molecular mechanisms of iron uptake in fungi
    Daniel J Kosman
    Department of Biochemistry, 140 Farber Hall, The University at Buffalo, School of Medicine and Biomedical Sciences, Buffalo, NY 14214, USA
    Mol Microbiol 47:1185-97. 2003
    ..The integration of these multiple uptake mechanisms and their regulation into over-all iron homeostasis in yeast concludes this brief review...
  77. pmc Transcription profiling of Candida albicans cells undergoing the yeast-to-hyphal transition
    Andre Nantel
    Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada H4P 2R2
    Mol Biol Cell 13:3452-65. 2002
    ..Thus Efg1p and Cph1p are needed for the activation of the transcriptional program that is induced by the presence of serum...
  78. pmc Development and characterization of an in vivo central venous catheter Candida albicans biofilm model
    D Andes
    Department of Medicine, University of Wisconsin, Madison, Wisconsin 53792, USA
    Infect Immun 72:6023-31. 2004
    ..albicans biofilm biology and drug resistance. In addition, the model provides a means to study novel drug therapies and device technologies targeted to the control of biofilm-associated infections...
  79. ncbi Mapping pathways and phenotypes by systematic gene overexpression
    Richelle Sopko
    Department of Medical Genetics and Microbiology, University of Toronto, 1 King s College Circle, Toronto, Ontario M5S 1A8, Canada
    Mol Cell 21:319-30. 2006
    ..Large-scale application of this approach should provide a strategy for identifying target molecules regulated by specific signaling pathways...
  80. pmc Role of the Hog1 stress-activated protein kinase in the global transcriptional response to stress in the fungal pathogen Candida albicans
    Brice Enjalbert
    Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom
    Mol Biol Cell 17:1018-32. 2006
    ..albicans has diverged from corresponding networks in model yeasts and that the C. albicans SAPK pathway functions in parallel with other pathways to regulate the core transcriptional response to stress...
  81. ncbi Termination of cryptic unstable transcripts is directed by yeast RNA-binding proteins Nrd1 and Nab3
    John T Arigo
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
    Mol Cell 23:841-51. 2006
    ..These results suggest that transcription termination of CUTs directed by Nrd1 and Nab3 is a prerequisite for rapid degradation by the nuclear exosome...
  82. pmc Calcineurin is essential for survival during membrane stress in Candida albicans
    M Cristina Cruz
    Department of Genetics, Duke University Medical Center, Durham, NC 27710, USA
    EMBO J 21:546-59. 2002
    ..albicans mutants, against other Candida species, or when combined with different azoles. We propose that calcineurin is part of a membrane stress survival pathway that could be targeted for therapy...
  83. pmc Genetic regulation of nitrogen metabolism in the fungi
    G A Marzluf
    Department of Biochemistry, Ohio State University, Columbus 43210, USA
    Microbiol Mol Biol Rev 61:17-32. 1997
    ..Nitrogen metabolic regulation appears to play a significant role in the pathogenicity of certain animal and plant fungal pathogens...
  84. ncbi Dissecting the regulatory circuitry of a eukaryotic genome
    F C Holstege
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Cell 95:717-28. 1998
    ....
  85. ncbi Genome-wide patterns of histone modifications in yeast
    Catherine B Millar
    Department of Biological Chemistry, Geffen School of Medicine and the Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
    Nat Rev Mol Cell Biol 7:657-66. 2006
    ..Such patterns might be important for the regulation of heterochromatin-mediated silencing, chromosome segregation, DNA replication and gene expression...
  86. pmc Transient transcriptional responses to stress are generated by opposing effects of mRNA production and degradation
    Ophir Shalem
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    Mol Syst Biol 4:223. 2008
    ..Our results show genome-wide interplay between mRNA production and degradation, and that alternative modes of such interplay determine the kinetics of the transcriptome in response to stress...
  87. pmc Parallel phenotypic analysis of sporulation and postgermination growth in Saccharomyces cerevisiae
    Adam M Deutschbauer
    Departments of Genetics and Biochemistry, Stanford University School of Medicine, CA 94305, USA
    Proc Natl Acad Sci U S A 99:15530-5. 2002
    ....
  88. pmc The fission yeast pmk1+ gene encodes a novel mitogen-activated protein kinase homolog which regulates cell integrity and functions coordinately with the protein kinase C pathway
    T Toda
    Cell Regulation Laboratory, Imperial Cancer Research Fund, London, United Kingdom
    Mol Cell Biol 16:6752-64. 1996
    ..pombe, Pmk1 may not act in a linear manner with respect to fission yeast protein kinase C homologs. Interestingly, however, these two pathways are not independent; instead, they regulate cell integrity in a coordinate manner...
  89. ncbi Regulation of multidrug resistance in pathogenic fungi
    Joachim Morschhäuser
    Institut für Molekulare Infektionsbiologie, Universitat Wurzburg, Rontgenring 11, D 97070 Wurzburg, Germany
    Fungal Genet Biol 47:94-106. 2010
    ..This review summarizes our current knowledge about efflux pump-mediated drug resistance and its regulation in human-pathogenic fungi...
  90. pmc Multiple roles of a heterotrimeric G-protein gamma-subunit in governing growth and development of Aspergillus nidulans
    Jeong Ah Seo
    Department of Food Microbiology and Toxicology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Genetics 171:81-9. 2005
    ..We propose that GpgA is the only A. nidulans G gamma-subunit and is required for normal vegetative growth as well as proper asexual and sexual developmental progression...
  91. pmc Distinct modes of regulation by chromatin encoded through nucleosome positioning signals
    Yair Field
    Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot, Israel
    PLoS Comput Biol 4:e1000216. 2008
    ....
  92. ncbi Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex
    Michael Christopher Keogh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 123:593-605. 2005
    ..This pathway apparently acts to negatively regulate transcription because deleting the genes for Set2 or Rpd3C(S) bypasses the requirement for the positive elongation factor Bur1/Bur2...
  93. ncbi Transcriptional response of Candida albicans to hypoxia: linkage of oxygen sensing and Efg1p-regulatory networks
    Eleonora R Setiadi
    Institut fur Mikrobiologie, Heinrich Heine Universitat, 40225 Dusseldorf, Germany
    J Mol Biol 361:399-411. 2006
    ..We discuss that C. albicans responds to hypoxia largely by different mechanisms compared to budding yeast and that hypoxic adaptation requiring Efg1p is crucial for successful infection of human cells and tissues...
  94. pmc Quorum sensing in the dimorphic fungus Candida albicans is mediated by farnesol
    J M Hornby
    School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588-0666, USA
    Appl Environ Microbiol 67:2982-92. 2001
    ..albicans supernatants, i.e., 50% GTF at ca. 30 to 35 microM. Nerolidol was ca. two times less active than farnesol. Neither geraniol (C(10)), geranylgeraniol (C(20)), nor farnesyl pyrophosphate had any QSM activity...
  95. pmc Evolutionary tinkering with conserved components of a transcriptional regulatory network
    Hugo Lavoie
    Biotechnology Research Institute, National Research Council, Montreal, Quebec, Canada
    PLoS Biol 8:e1000329. 2010
    ....
  96. ncbi DNA array studies demonstrate convergent regulation of virulence factors by Cph1, Cph2, and Efg1 in Candida albicans
    S Lane
    Department of Biological Chemistry, University of California, Irvine, California 92697-1700, USA
    J Biol Chem 276:48988-96. 2001
    ..Perhaps, C. albicans utilizes converging pathways to regulate its vital virulence factors to ensure its survival and pathogenicity in various host environments...
  97. pmc Transcriptional regulatory network for sexual differentiation in fission yeast
    Juan Mata
    Cancer Research UK Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge CB10 1HH, UK
    Genome Biol 8:R217. 2007
    ..The expression of hundreds of genes is modulated in successive waves during meiosis and sporulation in S. pombe, and several known transcription factors are critical for these processes...
  98. ncbi SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast
    S Strahl-Bolsinger
    Department of Biological Chemistry, University of California, Los Angeles 90095, USA
    Genes Dev 11:83-93. 1997
    ..RAP1 binding at the core region is unaffected by SIR3 overproduction and RAP1 shows no evidence of spreading. Thus, we propose that the structure of core telomeric heterochromatin differs from that extended by SIR3...
  99. ncbi Transcriptional repression by UME6 involves deacetylation of lysine 5 of histone H4 by RPD3
    S E Rundlett
    Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, California 90095, USA
    Nature 392:831-5. 1998
    ..As increased acetylation of this residue is not merely a consequence of gene transcription, acetylation of this site may be essential for regulating gene activity...
  100. pmc Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance
    J Chandra
    Center for Medical Mycology, University Hospitals of Cleveland, and Department of Dermatology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Bacteriol 183:5385-94. 2001
    ..The studies described here form the basis for investigations into the molecular mechanisms of Candida biofilm biology and antifungal resistance and provide the means to design novel therapies for biofilm-based infections...
  101. pmc Ammonia pulses and metabolic oscillations guide yeast colony development
    Zdena Palkova
    Department of Genetics and Microbiology, Charles University, Vinicna 5, 12844 Prague 2, Czech Republic
    Mol Biol Cell 13:3901-14. 2002
    ..These metabolic features characterize a novel survival strategy used by yeast under starvation conditions prevalent in nature...

Research Grants67

  1. Regulation of Drug Resistance Genes in C. Albicans
    Carol Kumamoto; Fiscal Year: 2007
    ..The long-term goal of these studies is to contribute to a more informed use of fluconazole with respect to prophylaxis, drug dosage regimens and the development of fluconazole resistant strains. [unreadable] [unreadable] [unreadable]..
  2. Regulation of Fungal Invasive Growth
    Carol Kumamoto; Fiscal Year: 2006
    ..cerevisiae. Experiments are proposed to identify interactors that bind to the gene products of interest and to elucidate the pathways in which these gene products participate. ..
  3. Intestinal colonization by C.albicans and enteric bacteria
    Carol Kumamoto; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  4. CRYPTOCOCCUS NEOFORMANS--A MODEL FOR PATHOGENIC FUNGI
    John Perfect; Fiscal Year: 2003
    ..This program emphasizes a focus on a single pathogen with a basic but broad-spectrum and highly interactive genomic approach to scientific investigations which can be extrapolated to other fungi. ..
  5. GENETIC & MOLECULAR BIOLOGY OF VIRULENCE IN C NEOFORMANS
    John Perfect; Fiscal Year: 2004
    ..It is our hypothesis that beginning to understand the genetic regulation of C. neoformans during infection through this proposal will be a powerful tool to find targets to interrupt pathogenesis. ..
  6. Transcriptional Integration of Metabolism
    ELTON YOUNG; Fiscal Year: 2007
    ..abstract_text> ..
  7. Role of the OPI1 gene in controlling viability of Candida glabrata
    TODD REYNOLDS; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  8. Conference on the Biology of Aging
    S Jazwinski; Fiscal Year: 2004
    ..abstract_text> ..
  9. GENETIC AND MOLECULAR DISSECTION OF THE NEUROSPORA CLOCK
    Jay Dunlap; Fiscal Year: 2007
    ..the organization of the cells as a function of time.[unreadable]..
  10. Analysis of Somatic Mutations in Cancer of the Kidney
    WAYNE VEDECKIS; Fiscal Year: 2005
    ..B) Is there a correlation between other suspected risk factors, hypertension and obesity, and p53 gene mutation prevalence and spectrum? (C) Is there a correlation between survival and p53 gene mutation prevalence and spectrum? ..
  11. Azoles and Candida in AIDS-Transcriptional Regulation
    THEODORE WHITE; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  12. Targeted 2nd generation sequencing in phenotyped Framingham & PGP populations.
    George M Church; Fiscal Year: 2010
    ..It will eventually also enable health providers to learn the genetic variations of their patients very inexpensively and thus help refine and improve their medical care. ..
  13. Detection and Understanding of Expression Differentiatio
    Lei Li; Fiscal Year: 2009
    ..To serve the scientific mission, we develop and integrate statistical and computational methodologies. Our research will benefit the society by new understanding of aging. ..
  14. PHOSPHOLIPID METABOLISM AND MEMBRANE FUNCTION
    GEORGE CARMAN; Fiscal Year: 2009
    ..Zinc transport function will be addressed with mutants defective in phospholipid synthesis, and with Zrtlp reconstituted into unilamellar vesicles with varying phospholipid composition. ..
  15. AIDS Related Candidiasis: Dynamic Expression of Drug Resistant Genes
    Theodore C White; Fiscal Year: 2010
    ..This has clinical significance, as fungi in a patient are never simply exposed to one defined set of drug/fungus conditions. ..
  16. In Vivo Expression of Candida Drug Resistance Genes
    THEODORE WHITE; Fiscal Year: 2004
    ..abstract_text> ..
  17. IDENTIFICATION AND ANALYSIS OF CLOCK CONTROLLED GENES
    Jennifer Loros; Fiscal Year: 2007
    ..These approaches will be illuminated by the insights afforded through our work with both a simple eukaryotic model system and a mammalian cell culture model of the clock. ..
  18. The role of the CCAAT-binding factor in Candida albicans
    DAVID MCNABB; Fiscal Year: 2007
    ..albicans virulence; and 3) to dissect the regulatory function of the CCAAT-binding factor. ..
  19. Azoles and Candida in AIDS - A Whole Cell Response
    THEODORE WHITE; Fiscal Year: 2004
    ....
  20. Characterization of a large protein interaction network
    Andreas Wagner; Fiscal Year: 2005
    ..The proposed work will put this speculation to an end for a key genetic network, some of whose conserved components are also involved in the etiology of human disease. ..
  21. AIDS Related Oral Candidiasis: Drugs, Sterols, and Fungal Cells
    Theodore C White; Fiscal Year: 2010
    ..Understanding the interactions between azoles and fungal cells is a clinically significant issue, with the potential for improving diagnosis, treatment and prevention of fungal infections and resistance. ..
  22. Protein-Facilitates RNA Catalysts
    KEVIN WEEKS; Fiscal Year: 2007
    ..5) To begin to explore the molecular mechanism by which Mrs1 facilitates catalysis of the bl3 group I intron. (6) To develop a high resolution model for the maturase-bl3 RNA complex. ..
  23. Mapping the Human Binary Interactome Network
    MARC contact VIDAL; Fiscal Year: 2010
    ..Our modified specific aims are to: i) Provide an expanded high-confidence/high-coverage version of the human binary interactome map ii) Validate human binary interaction data iii) Analyze the expanded human interactome model ..
  24. Connecting the Genome to the Cytoplasm
    Pamela A Silver; Fiscal Year: 2010
    ..The experiments proposed in this grant will not only lead to new basic insights but also to new ways to analyze on a genome-wide level the situation in genetically defective cells and certain cancers. ..
  25. An Oxygen-Sensing Network Involving Heme and Chaperones
    Li Zhang; Fiscal Year: 2009
    ..The gained knowledge should facilitate the understanding of the molecular mechanisms of oxygen sensing, heme signaling and chaperone action in higher eukaryotes. ..
  26. Genome-Wide Analysis of Adaptive Evolution in Humans
    JOSHUA MICHAEL AKEY; Fiscal Year: 2010
    ..Research that increases our understanding of the evolutionary forces that shape patterns of human genetic variation will facilitate disease gene mapping studies. ..
  27. Human RNAPII Structure/Function in Pausing & Elongation
    Robert Landick; Fiscal Year: 2009
    ..Our research is aimed at understanding these fundamental mechanisms of transcription elongation. The knowledge gained will increase understanding of the basic molecular biology of human diseases. ..
  28. Roles of MIF and iNOS in SLE-associate inflammation
    Michael Hickey; Fiscal Year: 2007
    ..Using rMIF, anti-MIF mAb, and anti-MIF small molecules, and by generating MRL/Ipr/MIF -/- mice, we will examine the role of MIF in the modulation of leukocyte-endothelial interactions in murine SLE. [unreadable] [unreadable]..
  29. Calcineurin and Aspergillus fumigatus Pathogenesis
    WILLIAM STEINBACH; Fiscal Year: 2008
    ..I will also test the non-immunosuppressive calcineurin inhibitor analogs to decrease the total level of immunosuppression and therefore increase the overall antifungal efficacy of exploiting the calcineurin pathway. ..
  30. Regulation and Gene Expression of Yeast Cytochrome c
    Fred Sherman; Fiscal Year: 2010
    ..Human NAT subunits are essential for development and are over-expressed in thyroid and hepatocellular carcinomas. ..
  31. Studies on start codon selection by eukaryotic ribosomes
    Katsura Asano; Fiscal Year: 2010
    ..gt;30 Ts- mutations mapping in rRNA obtained under an aim in the previous proposal will be used together with new mutations to be introduced at the predicted 40S subunit interface to mRNA. ..
  32. Regulation of Chromosome Segregation in Human Cells
    Prasad Jallepalli; Fiscal Year: 2009
    ..Such information is essential to developing more effective cancer therapies that attack the CIN phenotype found in most cancer cells. ..
  33. Echinocandin Resistance in Aspergillus
    Gregory S May; Fiscal Year: 2010
    ..Proof that these same mechanisms operate in A. fumigatus will then be developed through molecular genetic manipulation of genes in this fungus. ..
  34. DNA SEQUENCING OF UV INDUCED CHROMOSOMAL MUTATIONS
    Christopher Lawrence; Fiscal Year: 2003
    ..abstract_text> ..
  35. Regulation of gene expression by ribosomal frameshifting
    JONATHAN DINMAN; Fiscal Year: 2004
    ....
  36. Computation tools for analyzing gene expression data
    Naama Barkai; Fiscal Year: 2004
    ..Kinetic microarray experiments that highlight specific features of the transcriptional network will be designed, performed and analyzed. Methods for extracting regulatory relationships from these experiments will be developed. ..
  37. Identify compounds that target post-Golgi secretion(RMI)
    Edina Harsay; Fiscal Year: 2004
    ..We therefore propose to use a high-throughput, cell-based screen of a chemical library to identify compounds (and subsequently, their targets) that interfere specifically with one branch of the exocytic pathway. ..
  38. MOLECULAR PATHOGENESIS OF BLASTOMYCOSIS
    Bruce Klein; Fiscal Year: 2003
    ..Results from this work will yield important new information about a putative virulence factor, how it works, and how it's expression is regulated by the fungus and modulated by the host during infection. ..
  39. Natural Genetic Variation and Epistasis in Aging
    Daniel Promislow; Fiscal Year: 2002
    ..Finally, results from these pilot experiments will serve as the basis for a longer- term research proposal on the role that epistasis plays in the genetics of senescence. ..
  40. Role of Oxidative Mechanisms in the Toxicity of Metals
    SIMON AVERY; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  41. Methods for characterizing noncoding RNAs in bacteria
    Brian Tjaden; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  42. Microarray Expression Analysis of Burkholderia Mallei
    William Nierman; Fiscal Year: 2003
    ..Function and virulence validation of the identified virulence genes will be explored by construction and phenotype analysis of targeted knockout strains. ..
  43. CONTROLLING SIGNAL TRANSDUCTION WITH SYNTHETIC LIGANDS
    Stuart Schreiber; Fiscal Year: 2002
    ....
  44. The Transcriptome of Pathogenic Aspergillus Fumigatus
    William Nierman; Fiscal Year: 2003
    ..abstract_text> ..
  45. Mitochondrial Function and Aging in S. cerevisiae
    PAUL KIRCHMAN; Fiscal Year: 2003
    ..Results from these experiments will serve to clarify the role of variations in mitochondrial function in determining longevity. ..
  46. Zinc Transporters and Intracellular Zinc Trafficking
    David Eide; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  47. XENOBIOTIC-OXIDIZING P450 SYSTEM IN A WHITE ROT FUNGUS
    Jagjit Yadav; Fiscal Year: 2003
    ..chrysosporium constructed for this purpose, and/or in vitro assays using expressed enzymes. Optimized P450 conditions and strains will be tested in bench top models of bioremediation. ..
  48. Spatially regulated functions of G1 cyclins in yeast
    Mary Miller; Fiscal Year: 2003
    ..Elucidation of the mechanisms governing cell cycle progression by Gl-cyclins in budding yeast may significantly impact our understanding of cell cycle progression in higher eukaryotic systems. ..
  49. Characterization of the SARSCoV frameshift signal
    Jonathan D Dinman; Fiscal Year: 2010
    ....
  50. MECHANISM OF TRANSLESION REPLICATION IN EUKARYOTES
    Christopher Lawrence; Fiscal Year: 2003
    ..These plasmids have proved useful in detecting different kinds of recombinant events, a likely mechanism for the pathway. ..
  51. Molecular Targets of Novel Antifungal Compounds
    Ameeta Agarwal; Fiscal Year: 2008
    ..This work will provide a starting point in the development of new antifungal therapies. [unreadable] [unreadable] [unreadable]..
  52. The Role of Novel Peroxins in Peroxisomal Biogenesis
    John Aitchison; Fiscal Year: 2007
    ..Preliminary results applying these techniques demonstrate that the first these proteins is a previously unknown peroxin required for peroxisome size regulation and segregation during cell division. ..
  53. Identifying targets for diagnosing oral candidiasis
    David Schofield; Fiscal Year: 2005
    ..Since Candida species are the most important fungal pathogen of the oral cavity, the rapid and accurate diagnosis of oral candidiasis using novel disease biomarkers will significantly improve oral health. ..
  54. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    Odeniel Sertil; Fiscal Year: 2005
    ..This work will further our knowledge on what might be considered a type of metabolically induced differentiation and could open up a fairly extensive area of inquiry, possibly in antifungal drug development. ..
  55. Heme Action in Neuronogenesis and Oxygen Sensing
    Li Zhang; Fiscal Year: 2005
    ..These studies will define the role of heme in neuronogenesis and in oxygen sensing and should provide insights into the molecular mechanisms underlying the pathogenesis of heme deficiency-related diseases. ..
  56. Regulation of Burkholderia mallei virulence genes
    William Nierman; Fiscal Year: 2005
    ..It will provide critical information on virulence control and virulence mechanisms of B. mallei and will be crucial to the development of effective therapeutic interventions and vaccine strategies. ..
  57. Analysis of the Yeast Cell Cycle Transcriptional Program
    ALLEN FUTCHER; Fiscal Year: 2008
    ..In Aim 3, we will test the hypothesis that an oscillation in the state of histone acetylation is a major mechanism of cell cycle transcriptional control. [unreadable] [unreadable]..
  58. Genetic Determinants of Host Susceptibility to Pulmonary Anthrax
    Jagjit Yadav; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  59. The Causes and Consequences of Complementation and Selfishness in Viruses
    Sarah Joseph; Fiscal Year: 2008
    ..As a result, understanding these interactions in bacteriophage may provide insights into the evolution and pathogenesis of viral pathogens of humans. [unreadable] [unreadable] [unreadable]..
  60. GENETIC VARIABILITY OF THE YEAST CANDIDA ALBICANS
    Fred Sherman; Fiscal Year: 2004
    ..This work establishes for the first time a negative regulatory network for a secondary carbon source in an important pathogen. ..
  61. Analysis of SWI1, SWI2, amd SWI3 proteins
    Craig Peterson; Fiscal Year: 2005
    ..This aim also describes a conditional degron strategy to define the role(s) of individual SWI/SNF subunits for both in vivo and in vitro functions ..
  62. Light-regulated genes affecting the pathogen potential of Cryptococcus neoformans
    Alexander Idnurm; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  63. A DECONVOLUTION MICROSCOPE FOR CELL BIOLOGICAL RESEARCH
    Mark Rose; Fiscal Year: 2001
    ..By these means, the digital deconvolution microscope will provide outstanding research and educational opportunities on one of the most powerful optical microscopes available for use in the life sciences. ..
  64. GENETICS OF CENTRIN AND THE SPINDLE POLE BODY IN YEAST
    Mark Rose; Fiscal Year: 2003
    ..The MTOC proteins identified here may be important targets for anti-mitotic drugs for the treatment of cancer and fungal infections. ..
  65. Zeiss LSM 510 Meta Confocal Microsope
    Mark Rose; Fiscal Year: 2006
    ..Study of each of these biological systems will provide valuable information pertaining to significant human health problems such as birth defects, viral infection and cancer. [unreadable] [unreadable] [unreadable]..