bacterial gene expression regulation

Summary

Summary: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.

Top Publications

  1. pmc Regulatory RNAs in bacteria
    Lauren S Waters
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Cell 136:615-28. 2009
  2. ncbi Pathogenic Escherichia coli
    James B Kaper
    Center for Vaccine Development, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Nat Rev Microbiol 2:123-40. 2004
  3. ncbi A common mechanism of cellular death induced by bactericidal antibiotics
    Michael A Kohanski
    Center for BioDynamics and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA
    Cell 130:797-810. 2007
  4. ncbi The Listeria transcriptional landscape from saprophytism to virulence
    Alejandro Toledo-Arana
    Institut Pasteur, Unité des Interactions Bactéries Cellules, F 75015 Paris, France
    Nature 459:950-6. 2009
  5. ncbi The small RNA chaperone Hfq and multiple small RNAs control quorum sensing in Vibrio harveyi and Vibrio cholerae
    Derrick H Lenz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Cell 118:69-82. 2004
  6. ncbi Stochastic gene expression in a single cell
    Michael B Elowitz
    Laboratory of Cancer Biology, Center for Studies in Physics and Biology, Rockefeller University, New York, NY 10021, USA
    Science 297:1183-6. 2002
  7. ncbi Quorum sensing: cell-to-cell communication in bacteria
    Christopher M Waters
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
    Annu Rev Cell Dev Biol 21:319-46. 2005
  8. ncbi Bacterial iron homeostasis
    Simon C Andrews
    School of Animal and Microbial Sciences, University of Reading, Reading RG6 6AJ, UK
    FEMS Microbiol Rev 27:215-37. 2003
  9. pmc A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, Building 37, Room 5132, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:4620-5. 2002
  10. ncbi Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling
    Joanna C Betts
    Respiratory Pathogens, GlaxoSmithKline, Stevenage, Herts, UK
    Mol Microbiol 43:717-31. 2002

Research Grants

Detail Information

Publications332 found, 100 shown here

  1. pmc Regulatory RNAs in bacteria
    Lauren S Waters
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Cell 136:615-28. 2009
    ..Here, we review the known mechanisms and roles of regulatory RNAs, highlight emerging themes, and discuss remaining questions...
  2. ncbi Pathogenic Escherichia coli
    James B Kaper
    Center for Vaccine Development, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Nat Rev Microbiol 2:123-40. 2004
    ..Several different E. coli strains cause diverse intestinal and extraintestinal diseases by means of virulence factors that affect a wide range of cellular processes...
  3. ncbi A common mechanism of cellular death induced by bactericidal antibiotics
    Michael A Kohanski
    Center for BioDynamics and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA
    Cell 130:797-810. 2007
    ..g., reca...
  4. ncbi The Listeria transcriptional landscape from saprophytism to virulence
    Alejandro Toledo-Arana
    Institut Pasteur, Unité des Interactions Bactéries Cellules, F 75015 Paris, France
    Nature 459:950-6. 2009
    ..Together, our data unravel successive and coordinated global transcriptional changes during infection and point to previously unknown regulatory mechanisms in bacteria...
  5. ncbi The small RNA chaperone Hfq and multiple small RNAs control quorum sensing in Vibrio harveyi and Vibrio cholerae
    Derrick H Lenz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Cell 118:69-82. 2004
    ..We propose that Hfq, together with these sRNAs, creates an ultrasensitive regulatory switch that controls the critical transition into the high cell density, quorum-sensing mode...
  6. ncbi Stochastic gene expression in a single cell
    Michael B Elowitz
    Laboratory of Cancer Biology, Center for Studies in Physics and Biology, Rockefeller University, New York, NY 10021, USA
    Science 297:1183-6. 2002
    ..These results establish a quantitative foundation for modeling noise in genetic networks and reveal how low intracellular copy numbers of molecules can fundamentally limit the precision of gene regulation...
  7. ncbi Quorum sensing: cell-to-cell communication in bacteria
    Christopher M Waters
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
    Annu Rev Cell Dev Biol 21:319-46. 2005
    ....
  8. ncbi Bacterial iron homeostasis
    Simon C Andrews
    School of Animal and Microbial Sciences, University of Reading, Reading RG6 6AJ, UK
    FEMS Microbiol Rev 27:215-37. 2003
    ....
  9. pmc A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, Building 37, Room 5132, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:4620-5. 2002
    ....
  10. ncbi Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling
    Joanna C Betts
    Respiratory Pathogens, GlaxoSmithKline, Stevenage, Herts, UK
    Mol Microbiol 43:717-31. 2002
    ..Thus, we have generated a model with which we can search for agents active against persistent M. tuberculosis and revealed a number of potential targets expressed under these conditions...
  11. pmc Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system
    Stefan Pukatzki
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:1528-33. 2006
    ..Thus, the genes encoding the VAS-related, type VI secretion system likely play an important conserved function in microbial pathogenesis and represent an additional class of targets for vaccine and antimicrobial drug-based therapies...
  12. ncbi Persister cells, dormancy and infectious disease
    Kim Lewis
    Antimicrobial Discovery Center, Department of Biology, Northeastern University, 360 Huntington Avenue, Boston, Massachusetts 02115, USA
    Nat Rev Microbiol 5:48-56. 2007
    ..The molecular mechanisms that underlie the formation of dormant persister cells are now being unravelled and are the focus of this Review...
  13. ncbi Carbon catabolite repression in bacteria: many ways to make the most out of nutrients
    Boris Görke
    Department of General Microbiology, Institute of Microbiology and Genetics, Georg August University Gottingen, Grisebachstr 8, D 37077 Gottingen, Germany
    Nat Rev Microbiol 6:613-24. 2008
    ..In this Review, we discuss the most recent findings on the different mechanisms that have evolved to allow bacteria to use carbon sources in a hierarchical manner...
  14. pmc Identification, timing, and signal specificity of Pseudomonas aeruginosa quorum-controlled genes: a transcriptome analysis
    Martin Schuster
    Department of Microbiology and W M Keck Microbial Communities and Cell Signaling Program, University of Iowa, Iowa City, Iowa 52242, USA
    J Bacteriol 185:2066-79. 2003
    ..Acyl-homoserine lactone quorum sensing appears to be a system that allows ordered expression of hundreds of genes during P. aeruginosa growth in culture...
  15. ncbi Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen
    C K Stover
    PathoGenesis Corporation, Seattle, Washington 98119, USA
    Nature 406:959-64. 2000
    ..We propose that the size and complexity of the P. aeruginosa genome reflect an evolutionary adaptation permitting it to thrive in diverse environments and resist the effects of a variety of antimicrobial substances...
  16. pmc Genome-wide analysis of the general stress response network in Escherichia coli: sigmaS-dependent genes, promoters, and sigma factor selectivity
    Harald Weber
    Institut fur Biologie, Mikrobiologie, Freie Universitat Berlin, Königin Luise Str 12 16a, 14195 Berlin, Germany
    J Bacteriol 187:1591-603. 2005
    ..Thus, not only the expression of genes within a regulatory network but also the architecture of the network itself can be subject to regulation...
  17. pmc Large-scale mapping and validation of Escherichia coli transcriptional regulation from a compendium of expression profiles
    Jeremiah J Faith
    Bioinformatics Program, Boston University, Boston, Massachusetts, United States of America
    PLoS Biol 5:e8. 2007
    ..The compendium of expression data compiled in this study, coupled with RegulonDB, provides a valuable model system for further improvement of network inference algorithms using experimental data...
  18. pmc DBTBS: a database of transcriptional regulation in Bacillus subtilis containing upstream intergenic conservation information
    Nicolas Sierro
    Human Genome Center, The Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo, Japan
    Nucleic Acids Res 36:D93-6. 2008
    ..DBTBS can be accessed at: http://dbtbs.hgc.jp...
  19. ncbi P. aeruginosa quorum-sensing systems and virulence
    Roger S Smith
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Curr Opin Microbiol 6:56-60. 2003
    ..Therefore, via the regulation of multiple factors and the production of 3O-C(12)-HSL, quorum-sensing systems have a significant effect on the virulence of the bacteria and also on how the host responds to P. aeruginosa infections...
  20. pmc Structure and function of the feed-forward loop network motif
    S Mangan
    Departments of Molecular Cell Biology and Physics of Complex Systems, Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 100:11980-5. 2003
    ..Additional features, such as pulse generation and cooperativity, are discussed. This study defines the function of one of the most significant recurring circuit elements in transcription networks...
  21. pmc Quorum-sensing regulators control virulence gene expression in Vibrio cholerae
    Jun Zhu
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 99:3129-34. 2002
    ..Together these data suggest a role for quorum sensing in modulating expression of blocks of virulence genes in a reciprocal fashion in vivo...
  22. pmc How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria
    Josef Deutscher
    Microbiologie et Genetique Moleculaire, INRA CNRS INA PG UMR 2585, Thiverval Grignon, France
    Microbiol Mol Biol Rev 70:939-1031. 2006
    ..As we shall see, the PTS regulation network not only controls carbohydrate uptake and metabolism but also interferes with the utilization of nitrogen and phosphorus and the virulence of certain pathogens...
  23. ncbi Bistability in bacteria
    David Dubnau
    Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
    Mol Microbiol 61:564-72. 2006
    ..A review describing additional examples of bistability in bacteria has been published recently...
  24. pmc sigmaB modulates virulence determinant expression and stress resistance: characterization of a functional rsbU strain derived from Staphylococcus aureus 8325-4
    Malcolm J Horsburgh
    Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield, England S10 2TN
    J Bacteriol 184:5457-67. 2002
    ..We suggest that SarA is not likely to be the effector in the overall sigmaB-mediated effect on agr expression...
  25. pmc Phase and antigenic variation in bacteria
    Marjan W van der Woude
    Department of Microbiology, University of Pennsylvania, 202A Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104 6076, USA
    Clin Microbiol Rev 17:581-611, table of contents. 2004
    ..Finally, examples are presented to illustrate that phase-variable gene expression should be taken into account in the development of diagnostic assays and in the interpretation of experimental results and epidemiological studies...
  26. ncbi A genomic view of the human-Bacteroides thetaiotaomicron symbiosis
    Jian Xu
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Science 299:2074-6. 2003
    ..These and other expanded paralogous groups shed light on the molecular mechanisms underlying symbiotic host-bacterial relationships in our intestine...
  27. pmc Analysis of Pseudomonas aeruginosa 4-hydroxy-2-alkylquinolines (HAQs) reveals a role for 4-hydroxy-2-heptylquinoline in cell-to-cell communication
    Eric Déziel
    Department of Surgery, Harvard Medical School and Shriners Burns Institute, Massachusetts General Hospital, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 101:1339-44. 2004
    ..HAQ signaling represents a potential target for the pharmacological intervention of P. aeruginosa-mediated infections...
  28. pmc Quantitative characteristics of gene regulation by small RNA
    Erel Levine
    Center for Theoretical Biological Physics, University of California San Diego, La Jolla, California, United States of America
    PLoS Biol 5:e229. 2007
    ..Our results may also help guide the design of synthetic genetic circuits that have properties difficult to attain with protein regulators alone...
  29. ncbi Bacterial interference caused by autoinducing peptide variants
    G Ji
    Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, NY 10016, USA
    Science 276:2027-30. 1997
    ..Cross-inhibition of gene expression represents a type of bacterial interference that could be correlated with the ability of one strain to exclude others from infection or colonization sites, or both...
  30. pmc Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen
    Dragana Ajdic
    Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Proc Natl Acad Sci U S A 99:14434-9. 2002
    ....
  31. pmc Microarray analysis of Pseudomonas aeruginosa quorum-sensing regulons: effects of growth phase and environment
    Victoria E Wagner
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, New York 14642, USA
    J Bacteriol 185:2080-95. 2003
    ..Importantly, 222 genes were identified as being QS repressed. Environmental factors, such as medium composition and oxygen availability, eliminated detection of transcripts of many genes that were identified as being QS regulated...
  32. pmc Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria
    P W Postma
    E C Slater Institute, University of Amsterdam, The Netherlands
    Microbiol Rev 57:543-94. 1993
    ..In gram-positive bacteria, it has been demonstrated that HPr can be phosphorylated by ATP on a serine residue and this modification may perform a regulatory function...
  33. pmc RegulonDB (version 6.0): gene regulation model of Escherichia coli K-12 beyond transcription, active (experimental) annotated promoters and Textpresso navigation
    Socorro Gama-Castro
    Program of Computational Genomics, Centro de Ciencias Genomicas, Universidad Nacional Autonoma de Mexico, A P 565 A, Cuernavaca, Morelos 62100, Mexico
    Nucleic Acids Res 36:D120-4. 2008
    ..The data can be accessed through overviews of correlations about gene regulation. RegulonDB associated original literature, together with more than 4000 curation notes, can now be searched with the Textpresso text mining engine...
  34. ncbi Positive regulation of motility and flhDC expression by the RNA-binding protein CsrA of Escherichia coli
    B L Wei
    Department of Molecular Biology and Immunology, University of North Texas Health Science Center at Fort Worth, 3500 Camp Bowie Blvd, Fort Worth, TX 76107 2699, USA
    Mol Microbiol 40:245-56. 2001
    ..Thus, CsrA stimulates flhDC gene expression by a post-transcriptional mechanism reminiscent of its function in the repression of glycogen biosynthesis...
  35. pmc Translational control and target recognition by Escherichia coli small RNAs in vivo
    Johannes H Urban
    Max Planck Institute for Infection Biology, RNA Biology Group, Chariteplatz 1, 10117 Berlin, Germany
    Nucleic Acids Res 35:1018-37. 2007
    ..We expect our GFP fusion approach to be applicable to sRNA targets of other bacteria, and also demonstrate that Vibrio RyhB sRNA represses a Vibrio sodB fusion when co-expressed in E.coli...
  36. ncbi Regulation of gene expression by cell-to-cell communication: acyl-homoserine lactone quorum sensing
    C Fuqua
    Department of Biology, Indiana University, Bloomington, Indiana 47405, USA
    Annu Rev Genet 35:439-68. 2001
    ..Recent studies have begun to integrate acyl-HSL quorum sensing into global regulatory networks and establish its role in developing and maintaining the structure of bacterial communities...
  37. ncbi Engineering stability in gene networks by autoregulation
    A Becskei
    EMBL, Structures and Biocomputing, Heidelberg, Germany
    Nature 405:590-3. 2000
    ..Here we have designed and constructed simple gene circuits consisting of a regulator and transcriptional repressor modules in Escherichia coli and we show the gain of stability produced by negative feedback...
  38. ncbi Biofilms: the matrix revisited
    Steven S Branda
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Trends Microbiol 13:20-6. 2005
    ..This review discusses recent advances in our understanding of the extracellular matrix and its role in biofilm biology...
  39. pmc At the crossroads of bacterial metabolism and virulence factor synthesis in Staphylococci
    Greg A Somerville
    Department of Veterinary and Biomedical Sciences, University of Nebraska Lincoln, Lincoln, Nebraska 68583 0905, USA
    Microbiol Mol Biol Rev 73:233-48. 2009
    ....
  40. pmc Coupling of flagellar gene expression to flagellar assembly in Salmonella enterica serovar typhimurium and Escherichia coli
    G S Chilcott
    Department of Microbiology, University of Washington, Seattle, Washington 98195, USA
    Microbiol Mol Biol Rev 64:694-708. 2000
    ..Cell cycle control and flagellar development are codependent. We discuss how all these levels of regulation ensure efficient assembly of the flagellum in response to environmental stimuli...
  41. ncbi SOS response promotes horizontal dissemination of antibiotic resistance genes
    John W Beaber
    Department of Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, 136 Harrison Avenue, Boston, Massachusetts 02111, USA
    Nature 427:72-4. 2004
    ..Thus, we present a mechanism by which therapeutic agents can promote the spread of antibiotic resistance genes...
  42. pmc Transcriptional Adaptation of Mycobacterium tuberculosis within Macrophages: Insights into the Phagosomal Environment
    Dirk Schnappinger
    Department of Microbiology and Immunology, Cornell University, 1300 York Avenue, New York, NY 10021, USA
    J Exp Med 198:693-704. 2003
    ..The microbial transcriptome thus served as a bioprobe of the MTB phagosomal environment, showing it to be nitrosative, oxidative, functionally hypoxic, carbohydrate poor, and capable of perturbing the pathogen's cell envelope...
  43. pmc Functions required for extracellular quinolone signaling by Pseudomonas aeruginosa
    Larry A Gallagher
    Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA
    J Bacteriol 184:6472-80. 2002
    ..Others of the pleiotropic phenazine-minus mutations appear to inactivate novel components of the quorum-sensing regulatory network, including one regulator (np20) previously shown to be required for virulence in neutropenic mice...
  44. pmc Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like protein
    Maria A Schumacher
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR 97201 3098, USA
    EMBO J 21:3546-56. 2002
    ..Such binding suggests a mechanism for Hfq function...
  45. pmc Global gene expression in Staphylococcus aureus biofilms
    Karen E Beenken
    Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, 4301 W Markham, Little Rock, AR 72205, USA
    J Bacteriol 186:4665-84. 2004
    ..A primary theme that emerged from the analysis of these genes is that persistence within a biofilm requires an adaptive response that limits the deleterious effects of the reduced pH associated with anaerobic growth conditions...
  46. ncbi Optimality and evolutionary tuning of the expression level of a protein
    Erez Dekel
    Department of Molecular Cell Biology and Department of Physics of Complex Systems, The Weizmann Institute of Science, Rehovot 76100, Israel
    Nature 436:588-92. 2005
    ..Thus, protein expression from the lac operon seems to be a solution of a cost-benefit optimization problem, and can be rapidly tuned by evolution to function optimally in new environments...
  47. pmc Mistranslation of membrane proteins and two-component system activation trigger antibiotic-mediated cell death
    Michael A Kohanski
    Department of Biomedical Engineering, Center for BioDynamics, and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA
    Cell 135:679-90. 2008
    ....
  48. pmc Transcription profiling of the stringent response in Escherichia coli
    Tim Durfee
    Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Bacteriol 190:1084-96. 2008
    ..However, one-half of the up-regulated genes have unknown functions. How these results are correlated with the various effects of (p)ppGpp (in particular, RNA polymerase redistribution) is discussed...
  49. ncbi The transcriptional responses of Mycobacterium tuberculosis to inhibitors of metabolism: novel insights into drug mechanisms of action
    Helena I M Boshoff
    Tuberculosis Research Section, NIAID, National Institutes of Health, Rockville, Maryland 20852, USA
    J Biol Chem 279:40174-84. 2004
    ....
  50. ncbi Multistability in the lactose utilization network of Escherichia coli
    Ertugrul M Ozbudak
    Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 427:737-40. 2004
    ..The phase diagram thus serves as a sensitive probe of molecular interactions and as a powerful tool for rational network design...
  51. pmc Global analysis of the Mycobacterium tuberculosis Zur (FurB) regulon
    Anna Maciag
    Department of Genetics and Microbiology, University of Pavia, Pavia, Italy
    J Bacteriol 189:730-40. 2007
    ..tuberculosis infection...
  52. ncbi Optknock: a bilevel programming framework for identifying gene knockout strategies for microbial strain optimization
    Anthony P Burgard
    Department of Chemical Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biotechnol Bioeng 84:647-57. 2003
    ..Finally, the OptKnock procedure, by coupling biomass formation with chemical production, hints at a growth selection/adaptation system for indirectly evolving overproducing mutants...
  53. pmc Yersinia pestis--etiologic agent of plague
    R D Perry
    Department of Microbiology and Immunology, University of Kentucky, Lexington 40536, USA
    Clin Microbiol Rev 10:35-66. 1997
    ..These areas of research have provide new insights into the host-parasite relationship. This review will update our present understanding of the history, etiology, epidemiology, clinical aspects, and public health issues of plague...
  54. ncbi ppGpp: a global regulator in Escherichia coli
    Lisa U Magnusson
    Department of Cell and Molecular Biology Microbiology, Goteborg University, Box 462, 405 30 Goteborg, Sweden
    Trends Microbiol 13:236-42. 2005
    ..In addition, new insights into the mechanism by which ppGpp affects gene expression have been achieved owing to in vitro studies of ppGpp function, complemented by structural studies of the ppGpp-RNA polymerase complex...
  55. pmc Effect of RyhB small RNA on global iron use in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    J Bacteriol 187:6962-71. 2005
    ..Our results demonstrate the broad effects of a single noncoding RNA on iron homeostasis...
  56. pmc The Bacillus subtilis iron-sparing response is mediated by a Fur-regulated small RNA and three small, basic proteins
    Ahmed Gaballa
    Department of Microbiology, Cornell University, Ithaca, NY 14853 8101, USA
    Proc Natl Acad Sci U S A 105:11927-32. 2008
    ..Thus, iron deprivation in B. subtilis activates pathways to remodel the proteome to preserve iron for the most critical cellular functions...
  57. pmc Small RNAs encoded within genetic islands of Salmonella typhimurium show host-induced expression and role in virulence
    Gilly Padalon-Brauch
    Department of Molecular Genetics and Biotechnology, The Hebrew University Hadassah Medical School, 91120, Jerusalem, Israel
    Nucleic Acids Res 36:1913-27. 2008
    ..Our study suggests that the island encoded sRNA genes play an important role within the network that regulates bacterial adaptation to environmental changes and stress conditions and thus controls virulence...
  58. ncbi Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis
    John F Heidelberg
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Nat Biotechnol 20:1118-23. 2002
    ....
  59. pmc DsrA RNA regulates translation of RpoS message by an anti-antisense mechanism, independent of its action as an antisilencer of transcription
    N Majdalani
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 95:12462-7. 1998
    ..We propose that DsrA pairing stimulates RpoS translation by acting as an anti-antisense RNA, freeing the translation initiation region from the cis-acting antisense RNA and allowing increased translation...
  60. pmc Persisters: a distinct physiological state of E. coli
    Devang Shah
    Department of Biology, Northeastern University, 134 Mugar Hall, 360 Huntington Ave, Boston, MA 02115, USA
    BMC Microbiol 6:53. 2006
    ..Recent progress in understanding persisters is encouraging, but the main obstacle in understanding their nature was our inability to isolate these elusive cells from a wild-type population since their discovery in 1944...
  61. ncbi A network of networks: quorum-sensing gene regulation in Pseudomonas aeruginosa
    Martin Schuster
    Department of Microbiology, University of Washington, 1959 NE Pacific Street, HSB I 420, Seattle, WA 98195, USA
    Int J Med Microbiol 296:73-81. 2006
    ..Such epigenetic complexity may constitute the basis for the exceptional adaptability of P. aeruginosa to diverse environments...
  62. pmc RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Genes Dev 19:2176-86. 2005
    ..The formation of ribonucleoprotein complexes containing RNases could be a general way by which small RNAs destabilize target mRNAs in both prokaryotes and eukaryotes...
  63. ncbi Transcriptional regulation in constraints-based metabolic models of Escherichia coli
    Markus W Covert
    Department of Bioengineering, University of California, San Diego, La Jolla, California 92093 0412, USA
    J Biol Chem 277:28058-64. 2002
    ..This combined metabolic/regulatory model is thus an important step toward the goal of synthesizing genome-scale models that accurately represent E. coli behavior...
  64. ncbi Envelope stress responses and Gram-negative bacterial pathogenesis
    Tracy L Raivio
    Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada
    Mol Microbiol 56:1119-28. 2005
    ..This review highlights recent studies that indicate roles for the sigma(E), Cpx and Bae envelope stress responses in Gram-negative bacterial pathogenesis...
  65. pmc A chemosensory system that regulates biofilm formation through modulation of cyclic diguanylate levels
    Jason W Hickman
    Department of Microbiology, Box 357242, University of Washington, 1959 Northeast Pacific Street, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 102:14422-7. 2005
    ..Together, the data suggest that the wsp signal transduction pathway regulates biofilm formation through modulation of cyclic diguanylate levels...
  66. ncbi Coding sequence targeting by MicC RNA reveals bacterial mRNA silencing downstream of translational initiation
    Verena Pfeiffer
    Max Planck Institute for Infection Biology, RNA Biology Group, Berlin, Germany
    Nat Struct Mol Biol 16:840-6. 2009
    ..The discovery of CDS targeting markedly expands the sequence space for sRNA target predictions in bacteria...
  67. pmc Regulon and promoter analysis of the E. coli heat-shock factor, sigma32, reveals a multifaceted cellular response to heat stress
    Gen Nonaka
    Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143, USA
    Genes Dev 20:1776-89. 2006
    ..The intimate connection of the response to the membrane rationalizes why a major regulator of the response resides in that cellular compartment...
  68. ncbi Iron and metal regulation in bacteria
    K Hantke
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, D 72076, Tubingen, Germany
    Curr Opin Microbiol 4:172-7. 2001
    ..In organisms with Fur-dependent iron regulation, members of the DtxR family regulate manganese transport...
  69. ncbi The mechanisms of carbon catabolite repression in bacteria
    Josef Deutscher
    Laboratorie de Microbiologie et Génétique Moléculaire, INRA AgroParisTech CNRS, F 78850 Thiverval Grignon, France
    Curr Opin Microbiol 11:87-93. 2008
    ..PTS-independent CCR mechanisms are operative in several other bacteria...
  70. pmc A small RNA regulates multiple ABC transporter mRNAs by targeting C/A-rich elements inside and upstream of ribosome-binding sites
    Cynthia M Sharma
    RNA Biology Group, Max Planck Institute for Infection Biology, 10117 Berlin, Germany
    Genes Dev 21:2804-17. 2007
    ..Taken together, our study suggests highly conserved regions in sRNAs and mRNA regions distant from Shine-Dalgarno sequences as important elements for the identification of sRNA targets...
  71. pmc The use of gene clusters to infer functional coupling
    R Overbeek
    Mathematics and Computer Science Division, Argonne National Laboratory, 9700 S Cass Avenue, Argonne, IL 60439 4844, USA
    Proc Natl Acad Sci U S A 96:2896-901. 1999
    ....
  72. pmc Two GacA-dependent small RNAs modulate the quorum-sensing response in Pseudomonas aeruginosa
    Elisabeth Kay
    Département de Microbiologie Fondamentale, Universite de Lausanne, Building Biophore, CH 1015 Lausanne, Switzerland
    J Bacteriol 188:6026-33. 2006
    ..Both mutants showed increased swarming ability, azurin release, and early biofilm development...
  73. pmc Positive control of swarming, rhamnolipid synthesis, and lipase production by the posttranscriptional RsmA/RsmZ system in Pseudomonas aeruginosa PAO1
    Karin Heurlier
    Institut de Microbiologie Fondamentale, Universite de Lausanne, CH 1015 Lausanne, Switzerland
    J Bacteriol 186:2936-45. 2004
    ..Overexpression of rsmZ and a null mutation in rsmA resulted in quantitatively similar, negative or positive effects on target genes, in agreement with a model that postulates titration of RsmA protein by RsmZ RNA...
  74. ncbi Riboswitches in eubacteria sense the second messenger cyclic di-GMP
    N Sudarsan
    Howard Hughes Medical Institute, Yale University, New Haven, CT 06520, USA
    Science 321:411-3. 2008
    ..In addition, sequences matching the consensus for cyclic di-GMP riboswitches are present in the genome of a bacteriophage...
  75. pmc Agr-mediated dispersal of Staphylococcus aureus biofilms
    Blaise R Boles
    Department of Microbiology, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America
    PLoS Pathog 4:e1000052. 2008
    ..These findings indicate that induction of the agr system in established S. aureus biofilms detaches cells and demonstrate that the dispersal mechanism requires extracellular protease activity...
  76. pmc The C-terminal domain of Escherichia coli Hfq is required for regulation
    Branislav Vecerek
    Max F Perutz Laboratories, University of Vienna, Dr Bohrgasse 9, 1030 Vienna, Austria
    Nucleic Acids Res 36:133-43. 2008
    ..These studies indicate that the C-terminal extension of E. coli Hfq constitutes a hitherto unrecognized RNA interaction surface with specificity for mRNAs...
  77. ncbi Chitin induces natural competence in Vibrio cholerae
    Karin L Meibom
    Division of Infectious Diseases and Geographic Medicine, Department of Microbiology and Immunology, and Stanford Institute for the Environment, Stanford University, Stanford, CA 94305, USA
    Science 310:1824-7. 2005
    ....
  78. pmc A novel Staphylococcus aureus biofilm phenotype mediated by the fibronectin-binding proteins, FnBPA and FnBPB
    Eoghan O'Neill
    School of Biomolecular and Biomedical Science, University College Dublin, Ardmore House, Belfield, Dublin 4, Ireland
    J Bacteriol 190:3835-50. 2008
    ..These data identify a novel S. aureus biofilm phenotype promoted by FnBPA and FnBPB which is apparently independent of the known ligand-binding activities of these multifunctional surface proteins...
  79. ncbi Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA
    Anja Brencic
    Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 72:612-32. 2009
    ..Our work supports a model in which RsmA acts as a negative translational regulator, and where its positive effects are achieved indirectly by RsmA-mediated interference with translation of specific regulatory factors...
  80. pmc Targeting QseC signaling and virulence for antibiotic development
    David A Rasko
    Department of Microbiology, University of Texas UT Southwestern Medical Center, Dallas, TX 75390, USA
    Science 321:1078-80. 2008
    ..Inhibition of signaling offers a strategy for the development of broad-spectrum antimicrobial drugs...
  81. pmc Progression of primary pneumonic plague: a mouse model of infection, pathology, and bacterial transcriptional activity
    Wyndham W Lathem
    Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 102:17786-91. 2005
    ..pestis...
  82. ncbi A four-tiered transcriptional regulatory circuit controls flagellar biogenesis in Pseudomonas aeruginosa
    Nandini Dasgupta
    Department of Medicine Infectious Diseases, University of Florida, Gainesville, FL 32610, USA
    Mol Microbiol 50:809-24. 2003
    ..Two previously uncharacterized genes, which are coordinately regulated with known flagellar genes have been identified by genome-wide analysis and their role in flagellar biogenesis was analysed...
  83. ncbi The phosphate regulon and bacterial virulence: a regulatory network connecting phosphate homeostasis and pathogenesis
    Martin G Lamarche
    Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculte de Medecine Veterinaire, Universite de Montreal, Saint Hyacinthe, Quebec, Canada
    FEMS Microbiol Rev 32:461-73. 2008
    ..The Pho regulon is clearly not a simple regulatory circuit for controlling phosphate homeostasis; it is part of a complex network important for both bacterial virulence and stress response...
  84. ncbi The complete genome sequence of the gastric pathogen Helicobacter pylori
    J F Tomb
    Institute for Genomic Research, Rockville, Maryland 20850, USA
    Nature 388:539-47. 1997
    ..pylori has a few regulatory networks, and a limited metabolic repertoire and biosynthetic capacity. Its survival in acid conditions depends, in part, on its ability to establish a positive inside-membrane potential in low pH...
  85. ncbi Autoinduction and signal transduction in the regulation of staphylococcal virulence
    Richard P Novick
    Program in Molecular Pathogenesis, Skirball Institute, Department of Microbiology, New York University School of Medicine, 10016, USA
    Mol Microbiol 48:1429-49. 2003
    ..This review is an attempt to integrate a large body of data into the beginnings of a model that will hopefully help to guide research towards a full-scale test...
  86. ncbi H-NS: a modulator of environmentally regulated gene expression
    T Atlung
    Department of Chemistry and Life Sciences, Roskilde University, Denmark
    Mol Microbiol 24:7-17. 1997
    ..Finally, we summarize the evolutionary and functional relationship between H-NS and the homologous StpA...
  87. ncbi Iron uptake regulation in Pseudomonas aeruginosa
    Pierre Cornelis
    Department of Molecular and Cellular Interactions, Laboratory of Microbial Interactions, Flanders Interuniversity Institute for Biotechnology, Vrije Universiteit Brussel, Brussels, Belgium
    Biometals 22:15-22. 2009
    ..aeruginosa will be presented. An interesting feature revealed by this analysis of Fur-regulated genes is the overlap between the iron and the sulfur regulons as well with the quorum sensing system...
  88. pmc Microarray-based analysis of the Staphylococcus aureus sigmaB regulon
    Markus Bischoff
    Department of Medical Microbiology, University of Zurich, CH 8028 Zurich, Switzerland
    J Bacteriol 186:4085-99. 2004
    ..We propose that this alternative transcription factor may be of importance for the invading pathogen to fine-tune its virulence factor production in response to changing host environments...
  89. ncbi Biological control of soil-borne pathogens by fluorescent pseudomonads
    Dieter Haas
    Department of Fundamental Microbiology, University of Lausanne, CH 1015 Lausanne, Switzerland
    Nat Rev Microbiol 3:307-19. 2005
    ..Before engaging in these activities, biocontrol bacteria go through several regulatory processes at the transcriptional and post-transcriptional levels...
  90. pmc Multiple small RNAs act additively to integrate sensory information and control quorum sensing in Vibrio harveyi
    Kimberly C Tu
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Genes Dev 21:221-33. 2007
    ..Other regulators appear to be involved in control of V. harveyi qrr expression, allowing the integration of additional sensory information into the regulation of quorum-sensing gene expression...
  91. ncbi Nitrogen assimilation and global regulation in Escherichia coli
    Larry Reitzer
    Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, Texas 75080 0688, USA
    Annu Rev Microbiol 57:155-76. 2003
    ..Guanosine tetraphosphate appears to control Lrp synthesis. In summary, a network of interacting global regulators that senses different aspects of metabolism integrates nitrogen assimilation with other metabolic processes...
  92. ncbi Regulation of RpoS by a novel small RNA: the characterization of RprA
    N Majdalani
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bldg 37 Room 2E 18, Bethesda, MD 20892 4255, USA
    Mol Microbiol 39:1382-94. 2001
    ..The existence of two very different small RNA regulators of RpoS translation suggests that such additional regulatory RNAs are likely to exist, both for regulation of RpoS and for regulation of other important cellular components...
  93. ncbi Structure and function of the LysR-type transcriptional regulator (LTTR) family proteins
    Sarah E Maddocks
    Department of Oral and Dental Science, University of Bristol, Lower Maudlin Street, Bristol BS1 2LY, UK
    Microbiology 154:3609-23. 2008
    ....
  94. ncbi Sigma factors in Pseudomonas aeruginosa
    Eric Potvin
    Centre de Recherche sur la Fonction, Structure et Ingénierie des Protéines, Faculte de Medecine, Pavillon Charles Eugène Marchand, Universite Laval, Sainte Foy, Quebec, Canada
    FEMS Microbiol Rev 32:38-55. 2008
    ..aeruginosasigma and ECF are expected to provide new means to prevent infection, new targets for antimicrobial therapy, as well as new insights into the infection process...
  95. ncbi The cellular concentration of the sigma S subunit of RNA polymerase in Escherichia coli is controlled at the levels of transcription, translation, and protein stability
    R Lange
    Department of Biology, University of Konstanz, Germany
    Genes Dev 8:1600-12. 1994
    ....
  96. ncbi Functional specialization within the Fur family of metalloregulators
    Jin Won Lee
    Department of Microbiology, Cornell University, Wing Hall, Ithaca, NY 14853 8101, USA
    Biometals 20:485-99. 2007
    ..Despite numerous studies, the mechanism of metal ion sensing by Fur family proteins is still controversial. Other family members use metal catalyzed oxidation reactions to sense peroxide-stress (PerR) or the availability of heme (Irr)...
  97. ncbi A small, stable RNA induced by oxidative stress: role as a pleiotropic regulator and antimutator
    S Altuvia
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell 90:43-53. 1997
    ..Our results suggest that the oxyS RNA acts as a regulator that integrates adaptation to hydrogen peroxide with other cellular stress responses and helps to protect cells against oxidative damage...
  98. ncbi DnaE2 polymerase contributes to in vivo survival and the emergence of drug resistance in Mycobacterium tuberculosis
    Helena I M Boshoff
    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Twinbrook II, 12441 Parklawn Drive, Rockville, MD 20852, USA
    Cell 113:183-93. 2003
    ..These results may indicate a potential new target for therapeutic intervention...
  99. ncbi A novel two-component system controls the expression of Pseudomonas aeruginosa fimbrial cup genes
    Hemantha D Kulasekara
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115, USA
    Mol Microbiol 55:368-80. 2005
    ....
  100. ncbi A rough guide to the non-coding RNA world of Salmonella
    Jörg Vogel
    RNA Biology Group, Max Planck Institute for Infection Biology, Chariteplatz 1, D 10117 Berlin, Germany
    Mol Microbiol 71:1-11. 2009
    ....
  101. ncbi Escherichia coli acid resistance: tales of an amateur acidophile
    John W Foster
    Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile, Alabama 36695, USA
    Nat Rev Microbiol 2:898-907. 2004
    ..Surprisingly, certain microorganisms that grow at neutral pH have elegantly regulated systems that enable survival during excursions into acidic environments. The best-characterized acid-resistance system is found in E. coli...

Research Grants76

  1. REGULATION OF PYRIMIDINE GENE EXPRESSION IN BACTERIA
    CHARLES TURNBOUGH; Fiscal Year: 2001
    ..Although these studies are done with E. coli, the work is likely to be applicable to the study of gene expression and regulation in all bacteria and probably in eukaryotes, as well. ..
  2. GENETIC CONTROL OF NITRATE RESPIRATION IN E. COLI
    Valley J Stewart; Fiscal Year: 2010
    ..Thus, results from the model species E. coli enhance understanding for a broad range of pathogens that significantly impact public health. ..
  3. CHARACTERIZATION OF B ANTHRACIS EXOSPORIUM PROTEINS
    CHARLES TURNBOUGH; Fiscal Year: 2004
    ..anthracis spore surface (i.e., exosporium) to create affinity-matured single chain antibodies that neutralize exosporium protein activity, then test the effects of these reagents on spore properties. ..
  4. EFFLUX-MEDIATED RESISTANCE TO TETRACYCLINES
    Stuart Levy; Fiscal Year: 2006
    ..Such understanding will also enhance[unreadable] efforts to reverse efflux of other antibiotics and drugs used to treat infectious diseases, including[unreadable] bacteria, fungi and parasites, and cancer. ..
  5. In Vivo Study of Quorum Sensing in Vibrio cholerae
    Jun Zhu; Fiscal Year: 2006
    ..cholerae isolated from patients will be examined. The basic underlying goal of this proposal is apply the study of V. cholerae quorum sensing in vivo to the discovery of potential novel treatments for cholera disease. ..
  6. Search for Vibrio cholerae Quorum Sensing Genes
    Jun Zhu; Fiscal Year: 2004
    ..cholerae biotypes to determine if there is any correlation between the prevalence of quorum sensing systems and the emergence of epidemic strains. ..
  7. EFFLUX-MEDIATED RESISTANCE TO TETRACYCLINES
    Stuart Levy; Fiscal Year: 2004
    ....
  8. MULTIPLE ANTIBIOTIC RESISTANCE (MAR) REGULON
    Stuart Levy; Fiscal Year: 2001
    ....
  9. Bacterial Response to Singlet Oxygen
    Timothy Donohue; Fiscal Year: 2009
    ..The use of [1]O2 by eukaryotic cells to defend against pathogenic microbes and in photodynamic therapy to kill cancer cells predicts that our findings will have large antimicrobial and therapeutic potential. ..
  10. Role of Shigella two component regulation systems in intracellular adaptation
    Laura Runyen Janecky; Fiscal Year: 2007
    ..The research described in this proposal will be useful for designing more effective therapies and will yield information applicable to other similar pathogens. [unreadable] [unreadable] [unreadable] [unreadable]..
  11. Elucidating the Rickettsia prowazekii EnvZ/OmpR Regulon
    JONATHON AUDIA; Fiscal Year: 2007
    ..prowazekii as the model system. Understanding the role of gene regulation in pathogenesis may be critical to developing countermeasures and novel therapies against obligate intracellular Select Agents. [unreadable] [unreadable]..
  12. Measuring Metabolites using Riboswitch reports
    Ronald Breaker; Fiscal Year: 2006
    ..Moreover, bioinformatics searches can be expanded to uncover examples of known or new riboswitches in humans and other eukaryotes. ..
  13. Type III Secretion Inhibitors for Anti-Infective Therapy
    Donald Moir; Fiscal Year: 2007
    ..aeruginosa TTSS; (3) Screen a diverse compound library to identify and validate TTSS inhibitors; and (4) Prioritize validated screening hits for in vitro potency, mechanism, spectrum, and selectivity. [unreadable] [unreadable]..
  14. Environmental Regulation of Staphylococcus epidermidis PIA Synthesis
    GREG SOMERVILLE; Fiscal Year: 2007
    ..The work contained in this proposal is a first step toward inhibiting the formation of this complex sugar, preventing the formation of a bacterial biofilm, and providing physicians a way to treat patients. [unreadable] [unreadable]..
  15. DNA DAMAGE AND REPAIR IN SALMONELLA PATHOGENESIS
    Ferric Fang; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  16. MOLECULAR GENETIC ANALYSIS OF SALMONELLA PATHOGENICITY
    Roy Curtiss; Fiscal Year: 2006
    ..All experiments will be conducted under conditions that preclude infections of workers and inadvertent release of infectious microorganisms. ..
  17. Immunomodulation in infectious diarrhea
    Jan Michael Klapproth; Fiscal Year: 2007
    ....
  18. Oral Epithelial Cells: Innate Immune "Gatekeeper" of HIV
    MARK HERZBERG; Fiscal Year: 2006
    ..Innate immune factor-related genes may prove to be novel targets to prevent mucosal HIV. ..
  19. Metabolomics of the Virus-host Cell Interaction
    Joshua D Rabinowitz; Fiscal Year: 2010
    ..Such pathways, once identified, will be attractive new targets for antiviral therapy. ..
  20. Salmonella anti-influenza DNA & antigen delivery vaccine
    Roy Curtiss; Fiscal Year: 2008
    ..abstract_text> ..
  21. IN VIVO GENE EXPRESSION IN STAPHYLOCCUS AUREUS
    Ambrose Cheung; Fiscal Year: 2009
    ..Upon the completion of these studies, we hope to understand how sae is activated in vivo and what target genes sae impacts during infection. ..
  22. Structure/function analysis of the E. coli NIR/NRII signal transduction system
    Alexander Ninfa; Fiscal Year: 2009
    ..Our studies with a model system should allow rapid progress. ..
  23. Chemosensory Regulation of Bacterial Gene Expression
    John Kirby; Fiscal Year: 2009
    ..abstract_text> ..
  24. Analyses of sodium bioenergetics in Vibrio cholerae
    CLAUDIA C HASE; Fiscal Year: 2010
    ..cholerae ecology in the environment and in the lumen of hosts that will be applicable to a variety of bacterial species. ..
  25. Host-pathogen Genetics Using Listeria and Drosophila
    Nancy Freitag; Fiscal Year: 2008
    ..abstract_text> ..
  26. Identification of Metabolic Virulence Factors in the Epidemic Typhus Rickettsia
    JONATHON AUDIA; Fiscal Year: 2008
    ..prowazekii and are, thus, potential targets for the development of novel antimicrobials to treat this obligate intracytoplasmic pathogen. [unreadable] [unreadable] [unreadable] [unreadable]..
  27. Molecular pathogenesis of Francisella tularnesis
    YOUSEF A ABU KWAIK; Fiscal Year: 2010
    ..The bacterial effectors involved in exploiting the macrophage are potential targets for treatment as well as potential vaccine candidates. ..
  28. BRUCELLA STATIONARY PHASE GENE EXPRESSION AND VIRULENCE
    ROY M ROOP; Fiscal Year: 2010
    ..abortus 2308. ..
  29. Characterization of sar-agr Interactions in S. aureus
    AMBROSE LIN YAU CHEUNG; Fiscal Year: 2010
    ..Our goal is to understand how SarA controls target gene and how other intracellular regulators affect SarA expression in S. aureus. ..
  30. Targets for short-course TB therapy
    Christopher Sassetti; Fiscal Year: 2008
    ..Short-course therapy could impact the worldwide tuberculosis epidemic both by making treatment more widely available and by reducing the rate at which drug-resistant strains emerge. [unreadable] [unreadable] [unreadable]..
  31. Development of an additive model to study the significance of heat-labile and hea
    Weiping Zhang; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  32. INTERACTIONS OF THE SIGNAL TRANSDUCTION PROTEIN GLNK
    Alexander Ninfa; Fiscal Year: 2002
    ..These experiments should elucidate the design of the signal transduction system controlling nitrogen assimilation, and the mechanisms of interaction of PII and GlnK with their receptors and small molecule effectors. ..
  33. The Pore-forming Toxin of Legionella Pneumophila
    Yousef Abu Kwaik; Fiscal Year: 2003
    ..Our studies on L. pneumophila may provide a paradigm for other vacuolar intracellular pathogens such as Mycobacterium, Salmonella, and Chlamydia. ..
  34. Gene expression of Borrelia during Babesia coinfection
    James Coleman; Fiscal Year: 2004
    ..burgdorferi and B. microti alone and concurrently. ..
  35. Prevotella intermedia: Iron and Virulence
    JANINA LEWIS; Fiscal Year: 2004
    ..intermedia. Since P. intermedia is resistant to many antibiotics alternative methods of preventive measures are needed for this bacterium. ..
  36. Chlamydia pneumoniae vaccine candidates
    Bernhard Kaltenboeck; Fiscal Year: 2004
    ..pneumoniae vaccine candidate proteins in respiratory disease models using several inbred mouse strains. ..
  37. ENGINEERING ALLOSTERIC RIBOZYMES AND DEOXYRIBOZYMES
    Ronald Breaker; Fiscal Year: 2002
    ..In addition, we will test the function of these 'molecular switches' in vivo, to establish a new method of controlled gene expression. ..
  38. Characterization of Hemolysin A from Proteus mirabilis
    Todd Weaver; Fiscal Year: 2004
    ..The results may provide information regarding the hemolysin virulence factor within Proteus mirabilis and allow new drug design strategies for the treatment of Proteus-based urinary tract infections. ..
  39. Phenotype MicroArrays for Drug Toxicity Screening
    Barry Bochner; Fiscal Year: 2003
    ..In future studies (Phase II SBIR) we will expand the technology and scope of reference drugs tested to build a strong predictive database including cell lines representing more tissues and organs. ..
  40. Mid-Atlantic Microbial Pathogenesis Meeting(Conference)
    ROY ROOP; Fiscal Year: 2003
    ..A dedicated website (www.ecu.edu/mampm) has also been established to publicize the meeting and provide the necessary information and forms required for registration. ..
  41. MOLECULAR/GENETIC ANALYSIS OF BIOLOGICAL CLOCKS
    Carl Johnson; Fiscal Year: 2003
    ..The long-term research goal is to understand the mechanism of this fascinating clockwork. ..
  42. Virulence Factor of Porphyromonas gingivalis
    JANINA LEWIS; Fiscal Year: 2005
    ..Lewis' faculty development provides a highly supportive environment for a successful transition to the faculty phase of this award, and bodes well for her rapid development as an independent investigator. ..
  43. MOLECULAR/GENETIC ANALYSIS OF BIOLOGICAL CLOCKS IN CELLS
    Carl Johnson; Fiscal Year: 2005
    ..Downstream events in the transduction of entraining signals will be studied in mammalian cells (CREB/CBP, cADPR) and model systems (cADPR, ZGT gene) to characterize the phase-resetting pathway. ..
  44. Detection of Bioterrorism Agents with PM Technology
    Barry Bochner; Fiscal Year: 2006
    ..abstract_text> ..
  45. Antibiotic Susceptibility of Bacteria in Biofilms
    Philip Stewart; Fiscal Year: 2005
    ..This project will afford a rich interdisciplinary training experience for the three participating graduate students. ..
  46. Binding and regulation mechanism for S aureus
    Ambrose Cheung; Fiscal Year: 2006
    ..An understanding of these mechanisms will facilitate the development of novel agents that interfere with the function of the SarA family and the ensuing synthesis of cell wall adhesins and toxins. ..
  47. Protein Aspartate Phosphatases in Development
    Marta Perego; Fiscal Year: 2006
    ..The role of the Rap phosphatases not involved in sporulation will be investigated by microbiological and genetic approaches ..
  48. Plant Biofilm Inhibitors to Discover Biofilm Genes
    Thomas Wood; Fiscal Year: 2008
    ..deduce a model for bacterial biofilm formation based on the genetic information (which genes induced/repressed) and protein information discerned (via NMR) ..
  49. Iron Homeostasis in Salmonella-Host Interactions
    Ferric C Fang; Fiscal Year: 2010
    ..This work will have fundamental implications for understanding the strategies by which pathogenic microbes evade innate immunity by minimizing iron- dependent cell damage and maintaining iron homeostasis. ..
  50. Discovery of B. pseudomallei Therapeutics for Biodefense
    Donald Moir; Fiscal Year: 2008
    ..pseudomallei targets. In Phase III, a potent, safe, orally active B. pseudomallei inhibitor will be advanced into IND enabling toxicology and safety pharmacology studies and file an IND. [unreadable] [unreadable] [unreadable]..
  51. Ler control of EPEC virulence
    JAY MELLIES; Fiscal Year: 2008
    ..Further elucidation of the molecular mechanisms of Ler action should lead to the development of chemotherapeutic agents directed against EPEC and related E. coli O157 bacteria. [unreadable] [unreadable] [unreadable] [unreadable]..
  52. Cell-permeant Clock Proteins
    Carl Johnson; Fiscal Year: 2003
    ..abstract_text> ..
  53. Genetic Systems Bioengineering for Escherichia coli
    Alexander Ninfa; Fiscal Year: 2009
    ..In addition we propose designs for novel genetic clocks that test the role of various system design principles in the production of biological oscillations. ..
  54. Anaerobic Regulatory Pathways in a Periodontopathogen
    David Kolodrubetz; Fiscal Year: 2006
    ..Characterizing undefined, potentially new transcriptional regulatory pathways is important because the proteins involved may serve as targets for future drug development. ..
  55. Outer surface protein gene expression in B. burgdorferi
    D Samuels; Fiscal Year: 2003
    ..burgdorferi, which will contribute to the understanding of the basic biology of this pathogen and can lead to improved diagnostic, prevention and treatment strategies. ..
  56. Pathogenesis of EAST1 toxin in ETEC associated diarrhea disease
    Weiping Zhang; Fiscal Year: 2008
    ..The determination of virulence significance of EAST1 toxin in diarrheal disease will provide essential information to prevent or control this disease. [unreadable] [unreadable] [unreadable] [unreadable]..
  57. Airway Inflammation: Bacterial Epithelial Interactions
    BRYAN HURLEY; Fiscal Year: 2004
    ..Results obtained from the following proposal may shed light on diseases involving bacterial mediated lung inflammation such as cystic fibrosis and should also provide new strategies directed towards treating lung disease. ..
  58. Receptor-Mediated Endocytosis of Legionella Pneumophila
    Yousef Abu Kwaik; Fiscal Year: 2004
    ..Our studies may uncover potential pathogenic evolution of Lpn to invade the more evolved mammalian cells, and may contribute to the understanding of invasion of protozoa by Mycobacterium and Chlamydia. ..
  59. Genomic analysis of pediatric SIRS and septic shock
    Hector Wong; Fiscal Year: 2009
    ..Child health will be positively impacted by developing the capability of conducting zinc supplementation trials in a safe and rigorous manner. ..
  60. ANTIGEN DELIVERY SYSTEM DESIGN FOR HOST DEFENSE ANALYSIS
    Roy Curtiss; Fiscal Year: 2004
    ..As improved antigen delivery systems are discovered, it is anticipated that they will construct S. typhi or S. paratyphi A derivatives thereof for eventual clinical studies in humans. ..
  61. Rat, a regulator of autolysis in S. aureus
    Ambrose Cheung; Fiscal Year: 2008
    ..Our long-term goal is to promote autolysis with an anti-Rat or pro-SarV strategy without developing an untoward impact on virulence. ..
  62. SECRETION MECHANISM OF A NOVEL C. RECTUS S-LAYER PROTEIN
    David Kolodrubetz; Fiscal Year: 2002
    ..Finally, the transport mutants will be examined for the possible mis-localization of non-S-layer proteins since there are two reports of type I transport pathways transporting more than one virulence protein. ..
  63. Migraine Headache Treatment by Drug Aerosol Inhalation
    Joshua Rabinowitz; Fiscal Year: 2004
    ..Accomplishment of the goals of Phase II will lead directly to human clinical testing of a commercially viable device that could improve the lives of millions of people every year. ..
  64. S. typhimiurium Vaccine Against Bacterial Enteropathogens
    Roy Curtiss; Fiscal Year: 2007
    ..abstract_text> ..
  65. The Physiology of Oxidative Stress in Escherichia coli
    James Imlay; Fiscal Year: 2006
    ..coli will provide a blueprint for efforts to solve key issues in oxidative stress: obligate anaerobiosis, the killing mechanism of) hagocytes, and endogenous oxidative stress in higher organisms. ..
  66. Natural mRNA Genetic Switches that Bind Metabolites
    Ronald Breaker; Fiscal Year: 2006
    ..These findings will aid in the discovery of new riboswitches and will provide a solid foundation for the generation of designer genetic control elements based on RNA. ..
  67. Biofilm Formation by Listeria monocytogenes
    KATHERINE LEMON; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  68. REGULATION OF MICROBIAL NITROGEN METABOLISM
    ROBERT BENDER; Fiscal Year: 2006
    ..In particular, a lysine-sensitive positive effector of gdhA expression will be characterized and used as a model for the characterization of other positive effectors of gdhA. ..
  69. Stationary Phase Behavior in Oral Streptococci
    PATRICK PIGGOT; Fiscal Year: 2006
    ..These studies should lead to a better understanding of how S. mutans in mature Dental plaque biofilm persists and responds to fluctuations in its environment. ..
  70. Molecular mechanisms of X. nematophila-nematode interaction
    Heidi Goodrich Blair; Fiscal Year: 2007
    ..nematophila during colonization, further define a colonization-specific regulon, and expand our knowledge of the role of a ubiquitous transcription factor in the natural ecology of a bacterium. [unreadable] [unreadable]..
  71. Regulation of Streptococcus mutans Virulence by covR/S
    Grace Spatafora; Fiscal Year: 2003
    ..Taken collectively, these studies will elucidate S. mutans mechanism(s) of virulence gene control, and so enable prevention and/or intervention in the pathogenic process that leads to the development of Dental caries. ..
  72. H. Pylory factors associated with peptic ulcer disease
    TIMOTHY COVER; Fiscal Year: 2006
    ..pylori factors that help to determine clinical outcome, and ultimately, may lead to advances in the treatment or prevention of H. pylori-associated human diseases. [unreadable] [unreadable]..
  73. Development of a P.gingivalis vaccine
    Eric Reynolds; Fiscal Year: 2006
    ..The recombinant proteins, protein fragments and synthetic peptide multivalent constructs will be tested as defined vaccines against P. gingivalis in murine models of disease. ..
  74. MECAHNISMS OF ACTION OF C PERFRINGENS EXTEROTOXIN
    BRUCE MC CLANE; Fiscal Year: 2004
    ..abstract_text> ..
  75. SPORE PEPTIDOGLYCAN DEGRADATION IN BACILLUS ANTHRACIS
    DAVID POPHAM; Fiscal Year: 2005
    ....
  76. New approaches to studying host-chlamydia interactions
    Joanne Engel; Fiscal Year: 2006
    ..trachomatis infection. Together, these studies will significantly advance our knowledge of infectious disease pathogenesis and pave the way for new therapies and vaccines. ..