b lymphocyte gene rearrangement

Summary

Summary: Ordered rearrangement of B-lymphocyte variable gene regions coding for the immunoglobulin chains, thereby contributing to antibody diversity. It occurs during the differentiation of the immature B-lymphocyte.

Top Publications

  1. ncbi B cell development pathways
    R R Hardy
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Ave, Philadelphia, Pennsylvania 19111, USA
    Annu Rev Immunol 19:595-621. 2001
  2. ncbi AID and mismatch repair in antibody diversification
    Alberto Martin
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Chanin 403, Bronx, New York 10461, USA
    Nat Rev Immunol 2:605-14. 2002
  3. ncbi Somatic hyperconversion diversifies the single Vh gene of the chicken with a high incidence in the D region
    C A Reynaud
    Basel Institute for Immunology, Switzerland
    Cell 59:171-83. 1989
  4. pmc IMGT/LIGM-DB, the IMGT comprehensive database of immunoglobulin and T cell receptor nucleotide sequences
    Veronique Giudicelli
    IMGT, the international ImMunoGeneTics information system, Laboratoire d Immunogenetique moleculaire, LIGM, Universite Montpellier II, Institut de Genetique Humaine IGH, UPR CNRS 1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France
    Nucleic Acids Res 34:D781-4. 2006
  5. pmc IMGT/V-QUEST: the highly customized and integrated system for IG and TR standardized V-J and V-D-J sequence analysis
    Xavier Brochet
    IMGT, the international ImMunoGeneTics information system, Laboratoire d ImmunoGenetique Moleculaire LIGM, Universite Montpellier 2, Institut de Genetique Humaine IGH, UPR CNRS 1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France
    Nucleic Acids Res 36:W503-8. 2008
  6. ncbi The role of the non-homologous end-joining pathway in lymphocyte development
    Sean Rooney
    Howard Hughes Medical Institute, The Children s Hospital, The Department of Genetics, Harvard Medical School and The Center for Blood Research, Boston, MA 02115, USA
    Immunol Rev 200:115-31. 2004
  7. ncbi Rapid generation of specific antibodies by enhanced homologous recombination
    Hidetaka Seo
    Genetic Dynamics Research Unit, RIKEN Discovery Research Institute, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Nat Biotechnol 23:731-5. 2005
  8. ncbi Failure of B-cell differentiation in mice lacking the transcription factor EBF
    H Lin
    Howard Hughes Medical Institute, Department of Microbiology and Immunology, University of California, San Francisco 94143, USA
    Nature 376:263-7. 1995
  9. ncbi Functional immunoglobulin transgenes guide ordered B-cell differentiation in Rag-1-deficient mice
    E Spanopoulou
    Rockefeller University, New York, New York
    Genes Dev 8:1030-42. 1994
  10. ncbi Helix-loop-helix proteins and lymphocyte development
    Cornelis Murre
    Division of Biological Sciences, University of California, San Diego, La Jolla, California 92903, USA
    Nat Immunol 6:1079-86. 2005

Research Grants

  1. Assessment of BAFF as a mucosal HIV DNA vaccine adjuvant
    David Nemazee; Fiscal Year: 2005
  2. Immune Tolerance in Non-clonal Immune Systems
    David Nemazee; Fiscal Year: 2003
  3. Manipulating the IgE response with IgE-reactive macroself antigen
    David Nemazee; Fiscal Year: 2007
  4. Effect of BAFF on threshold of B cell tolerance
    David Nemazee; Fiscal Year: 2010
  5. B LYMPHOCYTE TOLERANCE IN HEALTH AND AUTOIMMUNITY
    David Nemazee; Fiscal Year: 2009
  6. RECEPTOR EDITING IN B CELLS
    David Nemazee; Fiscal Year: 2010
  7. IMMUNOGLOBULIN V GENES AND B CELL DEVELOPMENT
    Richard Hardy; Fiscal Year: 2006
  8. BD Biosciences LSR Flow Cytometer
    Richard Hardy; Fiscal Year: 2002
  9. Receptor Editing in Autoimmune and Normal Mice
    ANN FEENEY; Fiscal Year: 2009
  10. THE ROLE OF COMPLEMENT IN AUTOIMMUNITY
    Garnett Kelsoe; Fiscal Year: 2005

Detail Information

Publications176 found, 100 shown here

  1. ncbi B cell development pathways
    R R Hardy
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Ave, Philadelphia, Pennsylvania 19111, USA
    Annu Rev Immunol 19:595-621. 2001
    ....
  2. ncbi AID and mismatch repair in antibody diversification
    Alberto Martin
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Chanin 403, Bronx, New York 10461, USA
    Nat Rev Immunol 2:605-14. 2002
  3. ncbi Somatic hyperconversion diversifies the single Vh gene of the chicken with a high incidence in the D region
    C A Reynaud
    Basel Institute for Immunology, Switzerland
    Cell 59:171-83. 1989
    ..Allelic exclusion appears to be performed by restriction of a complete VDJ rearrangement to a single allele...
  4. pmc IMGT/LIGM-DB, the IMGT comprehensive database of immunoglobulin and T cell receptor nucleotide sequences
    Veronique Giudicelli
    IMGT, the international ImMunoGeneTics information system, Laboratoire d Immunogenetique moleculaire, LIGM, Universite Montpellier II, Institut de Genetique Humaine IGH, UPR CNRS 1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France
    Nucleic Acids Res 34:D781-4. 2006
    ..They represent the main source of IG and TR gene and allele knowledge stored in IMGT/GENE-DB and in the IMGT reference directory. IMGT/LIGM-DB is freely available at http://imgt.cines.fr...
  5. pmc IMGT/V-QUEST: the highly customized and integrated system for IG and TR standardized V-J and V-D-J sequence analysis
    Xavier Brochet
    IMGT, the international ImMunoGeneTics information system, Laboratoire d ImmunoGenetique Moleculaire LIGM, Universite Montpellier 2, Institut de Genetique Humaine IGH, UPR CNRS 1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France
    Nucleic Acids Res 36:W503-8. 2008
    ..The 'Advanced parameters' allow to modify default parameters used by IMGT/V-QUEST and IMGT/JunctionAnalysis according to the users' interest. IMGT/V-QUEST is freely available for academic research at http://imgt.cines.fr...
  6. ncbi The role of the non-homologous end-joining pathway in lymphocyte development
    Sean Rooney
    Howard Hughes Medical Institute, The Children s Hospital, The Department of Genetics, Harvard Medical School and The Center for Blood Research, Boston, MA 02115, USA
    Immunol Rev 200:115-31. 2004
    ..In this review, we discuss the factors that constitute this pathway as well as the evidence of their involvement in two lymphoid-specific DNA recombination events...
  7. ncbi Rapid generation of specific antibodies by enhanced homologous recombination
    Hidetaka Seo
    Genetic Dynamics Research Unit, RIKEN Discovery Research Institute, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Nat Biotechnol 23:731-5. 2005
    ....
  8. ncbi Failure of B-cell differentiation in mice lacking the transcription factor EBF
    H Lin
    Howard Hughes Medical Institute, Department of Microbiology and Immunology, University of California, San Francisco 94143, USA
    Nature 376:263-7. 1995
    ....
  9. ncbi Functional immunoglobulin transgenes guide ordered B-cell differentiation in Rag-1-deficient mice
    E Spanopoulou
    Rockefeller University, New York, New York
    Genes Dev 8:1030-42. 1994
    ..These data also support the hypothesis that Rag-1 directly participates in the V(D)J recombination process...
  10. ncbi Helix-loop-helix proteins and lymphocyte development
    Cornelis Murre
    Division of Biological Sciences, University of California, San Diego, La Jolla, California 92903, USA
    Nat Immunol 6:1079-86. 2005
    ..This review discusses HLH proteins in lymphocyte development and homeostasis...
  11. ncbi Computational tools for understanding sequence variability in recombination signals
    Lindsay G Cowell
    Department of Biostatistics and Bioinformatics, Center for Bioinformatics and Computational Biology, Duke University, Durham, NC, USA
    Immunol Rev 200:57-69. 2004
    ..These models successfully predict RS activity and identify functional, cryptic RSs (cRSs). These models permit studies to identify RSs and cRSs for empiric study and constitute a tool useful for understanding RS structure and function...
  12. pmc Human blood IgM "memory" B cells are circulating splenic marginal zone B cells harboring a prediversified immunoglobulin repertoire
    Sandra Weller
    Institut National de Santé et de la Recherche Médicale U373, Paris, France
    Blood 104:3647-54. 2004
    ..It is therefore proposed that these IgM(+)IgD(+)CD27(+) B cells provide the splenic marginal zone with a diversified and protective preimmune repertoire in charge of the responses against encapsulated bacteria...
  13. ncbi V(D)J recombination
    David G Schatz
    Howard Hughes Medical Institute, Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520 8011, USA
    Immunol Rev 200:5-11. 2004
  14. pmc Changes in histone acetylation are associated with differences in accessibility of V(H) gene segments to V-DJ recombination during B-cell ontogeny and development
    Kristen Johnson
    Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Mol Cell Biol 23:2438-50. 2003
    ..Thus, changes in histone acetylation appear to be important for both promotion and inhibition of V-DJ rearrangement during B-cell ontogeny and development...
  15. ncbi Mechanism and control of class-switch recombination
    John P Manis
    Children s Hospital, Center for Blood Research and Dept of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Trends Immunol 23:31-9. 2002
    ..In this review, we discuss the potential role of these factors in CSR, discuss potential relationships between CSR and somatic hypermutation, and speculate how CSR and related mechanisms might contribute to genomic instability...
  16. pmc Lineage specification and plasticity in CD19- early B cell precursors
    Lynn L Rumfelt
    Division of Basic Sciences, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    J Exp Med 203:675-87. 2006
    ..A) stage, and B/T lymphoid plasticity persists until the CD19+ pro-B stage. Thus, MLP, CLP, and Fr. A represent progressively B lineage-specified stages in development, before the CD19+ B lineage-committed pro-B stage...
  17. ncbi Essential roles of the kappa light chain intronic enhancer and 3' enhancer in kappa rearrangement and demethylation
    Matthew Inlay
    Section of Molecular Biology, Division of Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0322, USA
    Nat Immunol 3:463-8. 2002
    ..Our findings also indicate that the two kappa enhancers play overlapping and distinct roles in the demethylation of kappa in B cells...
  18. pmc Oncogenic transformation in the absence of Xrcc4 targets peripheral B cells that have undergone editing and switching
    Jing H Wang
    Howard Hughes Medical Institute, Harvard Medical School, MA 02115, USA
    J Exp Med 205:3079-90. 2008
    ..Thus, Xrcc4/p53-deficient pro-B lymphomas routinely activate c-myc by gene amplification, whereas Xrcc4/p53-deficient peripheral B cell lymphomas routinely ectopically activate a single c-myc copy...
  19. ncbi Frequent aberrant immunoglobulin gene rearrangements in pro-B cells revealed by a bcl-xL transgene
    W Fang
    Department of Medicine, University of Minnesota Medical School, Minneapolis 55455, USA
    Immunity 4:291-9. 1996
    ..This analysis also demonstrated that immunoglobulin gene rearrangement is less precise than previously appreciated...
  20. ncbi Functional transplant of megabase human immunoglobulin loci recapitulates human antibody response in mice
    M J Mendez
    Abgenix, Inc, Fremont, California 94555, USA
    Nat Genet 15:146-56. 1997
    ..Our results underscore the importance of the large Ig fragments with multiple V genes for restoration of a normal humoral immune response. These mice are likely to be a valuable tool for the generation of therapeutic antibodies...
  21. pmc Mechanisms promoting translocations in editing and switching peripheral B cells
    Jing H Wang
    Howard Hughes Medical Institute, USA
    Nature 460:231-6. 2009
    ..Our studies show peripheral B cells that attempt secondary V(D)J recombination, and determine a role for mechanistic factors in promoting recurrent translocations in tumours...
  22. pmc Stepwise activation of the immunoglobulin mu heavy chain gene locus
    D Chowdhury
    Rosenstiel Basic Medical Research Center and Department of Biology, Brandeis University, Waltham, MA 02454, USA
    EMBO J 20:6394-403. 2001
    ..We suggest that stepwise activation may be a general mechanism by which large segments of the genome are prepared for expression...
  23. pmc Evidence of a critical architectural function for the RAG proteins in end processing, protection, and joining in V(D)J recombination
    Chia Lun Tsai
    Department of Molecular Biophysics and Biochemistry, Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Genes Dev 16:1934-49. 2002
    ..Our results suggest that the RAG proteins have an architectural function in facilitating proper and efficient V(D)J joining, and a protective function in preventing degradation of broken ends prior to joining...
  24. pmc Receptor selection in B and T lymphocytes
    D Nemazee
    Department of Immunology, Scripps Research Institute, La Jolla, California 92037, USA
    Annu Rev Immunol 18:19-51. 2000
    ..New information about receptor editing in T cells and B-1 B cells is also discussed...
  25. ncbi Nonequivalent nuclear location of immunoglobulin alleles in B lymphocytes
    J A Skok
    Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 ONN, UK
    Nat Immunol 2:848-54. 2001
    ..However, this differential recruitment and expression of Ig alleles was not typically seen among transformed B cell lines. These data raise the possibility that epigenetic factors help maintain the monoallelic expression of Ig...
  26. pmc IMGT/V-QUEST, an integrated software program for immunoglobulin and T cell receptor V-J and V-D-J rearrangement analysis
    Veronique Giudicelli
    IMGT, the international ImMunoGeneTics information system, Laboratoire d Immunogenetique moleculaire, LIGM, Institut de Genetique Humaine IGH, UPR CNRS 1142, 141 rue de la Cardonille, F 34396 Montpellier Cedex 5, France
    Nucleic Acids Res 32:W435-40. 2004
    ..IMGT/V-QUEST is currently available for human and mouse, and partly for non-human primates, sheep, chondrichthyes and teleostei. IMGT/V-QUEST is freely available at http://imgt.cines.fr...
  27. ncbi Continued RAG expression in late stages of B cell development and no apparent re-induction after immunization
    W Yu
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10021, USA
    Nature 400:682-7. 1999
    ..Our findings may help to reconcile a series of apparently contradictory observations, and suggest a new model for the mechanisms that regulate allelic exclusion, receptor editing and tolerance...
  28. ncbi Receptor editing in lymphocyte development and central tolerance
    David Nemazee
    Department of Immunology, The Scripps Research Institute, Mail Drop IMM 29, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Rev Immunol 6:728-40. 2006
    ....
  29. pmc RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination
    Adam G W Matthews
    Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nature 450:1106-10. 2007
    ..Furthermore, our results provide the first evidence indicating that disrupting the read-out of histone modifications can cause an inherited human disease...
  30. ncbi Formation and resolution of double-strand break intermediates in V(D)J rearrangement
    D A Ramsden
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0540, USA
    Genes Dev 9:2409-20. 1995
    ..Efficient formation of signal junctions may require cell cycle progression, or down-regulation of the recombination machinery...
  31. pmc E47 is required for V(D)J recombinase activity in common lymphoid progenitors
    Lisa Borghesi
    Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    J Exp Med 202:1669-77. 2005
    ..Taken together, this work defines a role for E47 in regulating lineage progression at the CLP stage in vivo and describes the first transcription factor required for lineage-specific recombinase activity...
  32. ncbi RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination
    M A Oettinger
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142
    Science 248:1517-23. 1990
    ..RAG-1 and RAG-2 might activate the expression of the V(D)J recombinase but, more likely, they directly participate in the recombination reaction...
  33. pmc Hypermutation of immunoglobulin genes in memory B cells of DNA repair-deficient mice
    H Jacobs
    Basel Institute for Immunology, CH 4005 Basel, Switzerland
    J Exp Med 187:1735-43. 1998
    ..This appears also to be true for mismatch repair, RAD54-dependent double-strand-break repair, and AAG-mediated base excision repair...
  34. ncbi Aberrant B cell development and immune response in mice with a compromised BCR complex
    R M Torres
    Institute for Genetics, University of Cologne, Germany
    Science 272:1804-8. 1996
    ..B cells that do develop demonstrate a differential dependence on Ig-alpha signaling in antibody responses such that a signaling-competent Ig-alpha appears to be critical for the response to T-independent, but not T-dependent, antigens...
  35. ncbi Increased accumulation of hybrid V(D)J joins in cells expressing truncated versus full-length RAGs
    J A Sekiguchi
    Howard Hughes Medical Institute, Harvard University Medical School, Boston, MA 02115, USA
    Mol Cell 8:1383-90. 2001
    ..These results suggest a potential role for the non-core regions in repressing potentially harmful transposition events...
  36. ncbi Adoptive transfer of a superantigen-induced hole in the repertoire of natural IgM-secreting cells
    G J Silverman
    Department of Medicine, University of California San Diego, La Jolla, California 92093 0663, USA
    Cell Immunol 209:76-80. 2001
    ..These studies clarify the cellular mechanisms responsible for B-cell superantigen-induced modification of the repertoires of in vivo polyclonal B-cell populations...
  37. pmc Increased hypermutation at G and C nucleotides in immunoglobulin variable genes from mice deficient in the MSH2 mismatch repair protein
    Q H Phung
    Laboratory of Molecular Genetics, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Exp Med 187:1745-51. 1998
    ..The results suggest that the hypermutation pathway frequently mutates G.C pairs, and a MSH2-dependent pathway preferentially corrects mismatches at G and C...
  38. ncbi Antibody repertoires generated by VH replacement and direct VH to JH joining
    Sergei B Koralov
    The CBR Institute for Biomedical Research, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Immunity 25:43-53. 2006
    ..Thus, V(H) replacement is an efficient mechanism of antibody diversification, and its impact on the overall antibody repertoire could be greater than anticipated because it frequently leaves no molecular footprint...
  39. ncbi Localization of recombination activating gene 1/green fluorescent protein (RAG1/GFP) expression in secondary lymphoid organs after immunization with T-dependent antigens in rag1/gfp knockin mice
    H Igarashi
    Departments of Immunology, Pediatrics, and Cell Differentiation Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Japan
    Blood 97:2680-7. 2001
    ....
  40. ncbi Immunoglobulin heavy chain gene replacement: a mechanism of receptor editing
    C Chen
    Department of Molecular Biology, Princeton University, New Jersey 08544, USA
    Immunity 3:747-55. 1995
    ..The sd-tg model thus provides direct evidence for secondary rearrangement at VH-D-JH. This process may play a role in tolerance by editing autoreactive receptors and may also serve to diversify the VH repertoire...
  41. ncbi Long-term cultured E2A-deficient hematopoietic progenitor cells are pluripotent
    Tomokatsu Ikawa
    Division of Biological Sciences, 0377 University of California, San Diego, La Jolla, CA 92093, USA
    Immunity 20:349-60. 2004
    ..These observations indicate that E2A-deficient hematopoietic progenitor cells remain pluripotent after long-term culture in vitro and that E2A proteins play a critical role in B cell commitment...
  42. ncbi Both E12 and E47 allow commitment to the B cell lineage
    G Bain
    Department of Biology, University of California, San Diego, La Jolla 92093, USA
    Immunity 6:145-54. 1997
    ..Taken together, the data indicate that both E12 and E47 allow commitment to the B cell lineage and act synergistically to promote B lymphocyte maturation...
  43. pmc Inducible DNA breaks in Ig S regions are dependent on AID and UNG
    Carol E Schrader
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Exp Med 202:561-8. 2005
    ..Our results indicate that AID attacks cytosines on both DNA strands, and staggered breaks are processed to blunt DSBs at the initiating ss break sites. We propose a model to explain the types of end-processing events observed...
  44. pmc Identification and utilization of arbitrary correlations in models of recombination signal sequences
    Lindsay G Cowell
    Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA
    Genome Biol 3:RESEARCH0072. 2002
    ..The method places no restrictions on the number of correlated positions or on their spatial relationship within the site. No prior empirical evidence for the correlation structure is necessary...
  45. ncbi EBV-infected B cells in infectious mononucleosis: viral strategies for spreading in the B cell compartment and establishing latency
    J Kurth
    Institute for Genetics and Department of Internal Medicine I, University of Cologne, Germany
    Immunity 13:485-95. 2000
    ....
  46. ncbi The NF-kappaB canonical pathway is involved in the control of the exonucleolytic processing of coding ends during V(D)J recombination
    M Margarida Souto-Carneiro
    Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892 1560, USA
    J Immunol 180:1040-9. 2008
    ..However, selection of the primary B cell repertoire appeared to be intact and was partially able to correct the defects generated by abnormal V(D)J recombination...
  47. ncbi Role of receptor editing and revision in shaping the B and T lymphocyte repertoire
    V Kouskoff
    Mount Sinai Hospital, Institute of Gene Therapy, New York, New York 10029, USA
    Life Sci 69:1105-13. 2001
    ..Recent studies have likewise suggested a role for receptor editing and revision in shaping the T cell repertoire during development and tolerance...
  48. ncbi Allelic exclusion of immunoglobulin gene rearrangement and expression: why and how?
    Mark Schlissel
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3200, USA
    Semin Immunol 14:207-212; discussion 225-6. 2002
    ..This review will suggest an hypothesis to answer the 'why bother' aspect of allelic exclusion and then go on to propose a mechanism, distinct from feedback regulation, which may contribute to the allelic exclusion of Ig gene expression...
  49. pmc The role of components of recombination signal sequences in immunoglobulin gene segment usage: a V81x model
    M Larijani
    Department of Immunology and Ontario Cancer Institute, University of Toronto, Room 8 111, 610 University Avenue, Toronto, Ontario M5G 2M9, Canada
    Nucleic Acids Res 27:2304-9. 1999
    ..Furthermore, the contribution of the heptamer and nonamer to differential VHusage in our assay is correlated inversely with their conservation throughout the VHlocus...
  50. ncbi Rearrangement-enhancing element upstream of the mouse immunoglobulin kappa chain J cluster
    L Ferradini
    INSERM U373, Institut Necker, Faculte de Medecine, Universite Paris V, France
    Science 271:1416-20. 1996
    ..This cis-acting recombination-enhancing element affects the rearrangement process without being involved in regulating transcription...
  51. ncbi B-cell repertoire formation: role of the recombination signal sequence in non-random V segment utilization
    A J Feeney
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    Immunol Rev 175:59-69. 2000
    ..We also describe experiments showing that the sequence of the spacer of the RSS can play an important role in relative recombination frequencies...
  52. ncbi Use of immunoglobulin variable-region genes by normal subjects and patients with systemic lupus erythematosus
    A Hansen
    Outpatients Clinic, Berlin, Germany
    Int Arch Allergy Immunol 123:36-45. 2000
    ....
  53. ncbi Improved methods for detecting selection by mutation analysis of Ig V region sequences
    Uri Hershberg
    Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06520, USA
    Int Immunol 20:683-94. 2008
    ..These results show that selection can be detected in vivo with high specificity using the new method proposed here, allowing greater insight into the existence and direction of antigen-driven selection...
  54. ncbi Targeted disruption of the Artemis murine counterpart results in SCID and defective V(D)J recombination that is partially corrected with bone marrow transplantation
    Lanying Li
    Department of Pediatrics, University of California, San Francisco, CA 94143, USA
    J Immunol 174:2420-8. 2005
    ....
  55. ncbi Anti-peripherin B lymphocytes are positively selected during diabetogenesis
    Jorge Carrillo
    Laboratory of Immunobiology for Research and Application to Diagnosis and Center for Transfusion and Tissue Bank BST, Institut d Investigacio Germans Trias i Pujol, Badalona, Barcelona, Spain
    Mol Immunol 45:3152-62. 2008
    ....
  56. pmc Mice reconstituted with DNA polymerase beta-deficient fetal liver cells are able to mount a T cell-dependent immune response and mutate their Ig genes normally
    G Esposito
    Institute for Genetics, University of Cologne, D 50931 Cologne, Germany
    Proc Natl Acad Sci U S A 97:1166-71. 2000
    ....
  57. ncbi Lack of N regions in antigen receptor variable region genes of TdT-deficient lymphocytes
    T Komori
    Howard Hughes Medical Institute, Children s Hospital, Boston, MA 02115
    Science 261:1171-5. 1993
    ....
  58. pmc Antigen specificity of clonally expanded and receptor edited cerebrospinal fluid B cells from patients with relapsing remitting MS
    Doris Lambracht-Washington
    Department of Neurology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Neuroimmunol 186:164-76. 2007
    ..Targeting the mechanisms that allow these self-reactive B cells to reside in the CSF of MS patients may prove to be a potent immunotherapeutic strategy...
  59. ncbi Utilization of polymerase chain reaction on archival cytologic material: a comparison with fresh material with special emphasis on cerebrospinal fluids
    Rubina Mattu
    Laboratory of Pathology, National Cancer Institute, Bethesda, MD 20892, USA
    Mod Pathol 17:1295-301. 2004
    ..SSL of archival cytologic material can be used with excellent results for molecular diagnostic tests when fresh material is not available or when the cytologic diagnosis needs further clarification...
  60. ncbi The chicken B-cell receptor complex and its role in avian B-cell development
    C E Sayegh
    Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada
    Immunol Rev 175:187-200. 2000
    ....
  61. ncbi Rectification of age-related impairment in Ig gene hypermutation during a memory response
    Shuhua Han
    Department of Immunology, Baylor College of Medicine, Houston, TX 77030, USA
    Int Immunol 16:525-32. 2004
    ..These findings may have important implications in designing vaccines and immunization protocols for the elderly population and patients with certain immune deficiencies such as AIDS...
  62. ncbi RAGs and regulation of autoantibodies
    Mila Jankovic
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10021, USA
    Annu Rev Immunol 22:485-501. 2004
    ..Here we review the prevalence of autoantibodies in the initial antibody repertoire, their regulation by receptor editing, and the role of the recombinase proteins (RAG1 and RAG2) in this process...
  63. ncbi V(D)J recombination frequencies can be profoundly affected by changes in the spacer sequence
    Alina Montalbano
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Immunol 171:5296-304. 2003
    ..These data demonstrate that the spacer sequence should be considered to play an important role in the recombination efficacy of an RSS, and that the effect of the spacer occurs primarily subsequent to RAG binding...
  64. ncbi Nucleic acid structures and enzymes in the immunoglobulin class switch recombination mechanism
    Kefei Yu
    Department of Pathology, Norris Comprehensive Cancer Ctr, Rm 5428, 1441 Eastlake Ave, MC9176, Los Angeles, CA 90033, USA
    DNA Repair (Amst) 2:1163-74. 2003
    ..Progress has been made on the enzymes involved in leading to the DNA cleavage events and on identifying the unusual DNA structures that those enzymes recognize...
  65. ncbi Cell biology, molecular embryology, Lamarckian and Darwinian selection as evolvability
    H Hoenigsberg
    Instituto de Genetica Evolutiva y Biologia Molecular, Universidad Manuela Beltran, Bogota, DC, Colombia
    Genet Mol Res 2:7-28. 2003
    ..It seems that molecular Lamarckism can survive (Lewin, 1993)...
  66. ncbi Antibody diversification by somatic mutation: from Burnet onwards
    Michael S Neuberger
    1Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
    Immunol Cell Biol 86:124-32. 2008
    ....
  67. ncbi Nonoverlapping functions of DNA polymerases mu, lambda, and terminal deoxynucleotidyltransferase during immunoglobulin V(D)J recombination in vivo
    Barbara Bertocci
    INSERM U783, Faculté de Médecine René Descartes, Site Necker Enfants Malades, 156 rue de Vaugirard, 75730 Paris Cedex 15, France
    Immunity 25:31-41. 2006
    ....
  68. ncbi Mice lacking TdT: mature animals with an immature lymphocyte repertoire
    S Gilfillan
    Laboratoire de Genetique Moleculaire des Eucaryotes du CNRS, Institut de Chimie Biologique, Strasbourg, France
    Science 261:1175-8. 1993
    ..Thus, switch-on of the TdT gene during the first week after birth provokes an even greater expansion of lymphocyte receptor diversity than had previously been thought...
  69. ncbi Homology-directed recombination in IgH variable region genes from human neonates, infants and adults: implications for junctional diversity
    Karl Bauer
    Department of Pediatrics, JW Goethe University, Frankfurt, Germany
    Mol Immunol 44:2969-77. 2007
    ..Therefore, unlike in mice, homology-directed recombination does not cause junctional uniformity in human neonates...
  70. ncbi Allelic 'choice' governs somatic hypermutation in vivo at the immunoglobulin kappa-chain locus
    Shira Fraenkel
    The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Nat Immunol 8:715-22. 2007
    ..Thus, it seems that the epigenetic mechanisms that initially bring about monoallelic variable-(diversity)-joining rearrangement continue to be involved in the control of antibody diversity at later stages of B cell development...
  71. ncbi Primary cutaneous lymphoproliferative disorders with dual lineage rearrangement
    Dmitry V Kazakov
    Sikl s Department of Pathology, Charles University, Medical Faculty Hospital, Pilsen, Czech Republic
    Am J Dermatopathol 28:399-409. 2006
    ..Further reports are needed to identify these lesions to clarify their nature and biologic potential...
  72. ncbi Accessibility control of V(D)J recombination
    Robin Milley Cobb
    Department of Microbiology and Immunology, Vanderbilt University, Nashville, Tennessee, USA
    Adv Immunol 91:45-109. 2006
    ..The discussion will focus on cis-acting regulation by ACEs, the nuclear factors that control ACE function, and the epigenetic modifications that establish recombinase accessibility...
  73. ncbi Normal D-JH rearranged products of the IgH gene in SCID mouse bone marrow
    R Araki
    Cell Biology Laboratory, Department of Radiobiology, Radiation Effects Research Foundation, 8 6 Nakagawa 1 chome, Nagasaki 850, Japan
    Int Immunol 8:1045-53. 1996
    ..Furthermore, the features of rearranged products isolated from SCID bone marrow cells were remarkably similar to those from leaky SCID mice...
  74. ncbi V(D)J recombination activates a p53-dependent DNA damage checkpoint in scid lymphocyte precursors
    C J Guidos
    Division of Immunology and Cancer, Hospital for Sick Children Research Institute, Toronto, Ontario, Canada
    Genes Dev 10:2038-54. 1996
    ..These results demonstrate that a p53-mediated DNA damage checkpoint contributes to the immune deficiency characteristic of the scid mutation and limits the oncogenic potential of DSBs generated during V(D)J recombination...
  75. ncbi Frequent occurrence of B-cell lymphomas in angioimmunoblastic T-cell lymphoma and proliferation of Epstein-Barr virus-infected cells in early cases
    Klaus Willenbrock
    Senckenbergisches Institut für Pathologie, Klinikum der Johann Wolfgang Goethe Universität Frankfurt am Main, Frankfurt am Main, Germany
    Br J Haematol 138:733-9. 2007
    ..This might be relevant in the development of secondary lymphomas...
  76. ncbi Two consecutive immunophenotypic switches in a child with MLL-rearranged acute lymphoblastic leukemia
    Giuseppe Germano
    Laboratory of Pediatric Onco Hematology, Department of Pediatrics, University Hospital of Padua, Padua, Italy
    Haematologica 91:ECR09. 2006
    ..The high expression of HOXA9 and FLT3 genes remaining genotypically stable in a leukemia throughout phenotypic switches, suggests that this leukemia may have originated as a common B/myeloid progenitors...
  77. pmc Acetylation increases access of remodelling complexes to their nucleosome targets to enhance initiation of V(D)J recombination
    Karl P Nightingale
    Institute of Biomedical Research, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Nucleic Acids Res 35:6311-21. 2007
    ....
  78. pmc The analysis and quantification of a clonal B cell response in a hyperimmunized anti-D donor
    S E Dohmen
    Sanquin Research, Amsterdam and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Plesmanlaan 125, 10066 CX Amsterdam, The Netherlands
    Clin Exp Immunol 144:223-32. 2006
    ..Furthermore, we found a high frequency of clonal B cells. These results contribute to the understanding of the immune response against RhD in hyperimmunized anti-D donors...
  79. ncbi Immunoglobulin light chain gene rearrangements in precursor-B-acute lymphoblastic leukemia: characteristics and applicability for the detection of minimal residual disease
    Vincent H J van der Velden
    Department of Immunology, Erasmus MC, Rotterdam, The Netherlands
    Haematologica 91:679-82. 2006
    ..Our data show that Vk-Jk and Vlambda-Jlambda rearrangements can be successfully applied for MRD detection in a subset of patients with precursor-B-ALL...
  80. ncbi Regulation of IgH gene assembly: role of the intronic enhancer and 5'DQ52 region in targeting DHJH recombination
    Roshi Afshar
    Department of Microbiology Immunology, Vanderbilt University Medical School, Nashville, TN 37232, USA
    J Immunol 176:2439-47. 2006
    ....
  81. ncbi Rearrangement/hypermutation/gene conversion: when, where and why?
    J C Weill
    INSERM U373, Faculte de Medecine Necker, Enfants Malades, Universite Paris, France
    Immunol Today 17:92-7. 1996
    ..They propose that somatic mutation and gene conversion represent analogous molecular strategies occurring in specific chromatin accessibility contexts...
  82. pmc Human B cells accumulate immunoglobulin V gene somatic mutations in a cell contact-dependent manner in cultures supported by activated T cells but not in cultures supported by CD40 ligand
    S C Huang
    Virginia Mason Research Center Department of Immunology, University of Washington, Seattle, WA, USA
    Clin Exp Immunol 116:441-8. 1999
    ..Cell-cell contact was required for mutation because mutations were not detected when B cells were separated from activated T cells by a membrane...
  83. ncbi Molecular immunoglobulin/T- cell receptor clonality analysis in cutaneous lymphoproliferations. Experience with the BIOMED-2 standardized polymerase chain reaction protocol
    Yorick Sandberg
    Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
    Haematologica 88:659-70. 2003
    ..The obtained PCR products were subjected to heteroduplex (HD) and GeneScan (GS) analysis. We compared the data of 154 samples with the histopathologic diagnosis, based on the EORTC classification of skin lymphomas...
  84. ncbi Comparative analyses of immunoglobulin genes: surprises and portents
    Martin F Flajnik
    Department of Microbiology and Immunology, University of Maryland at Baltimore, Baltimore, Maryland 21201, USA
    Nat Rev Immunol 2:688-98. 2002
    ....
  85. ncbi Expression of ZAP-70 is associated with increased B-cell receptor signaling in chronic lymphocytic leukemia
    Liguang Chen
    Division of Hematology Oncology, Department of Medicine, University of California, San Diego for the CLL Research Consortium, San Diego, CA, USA
    Blood 100:4609-14. 2002
    ....
  86. ncbi V(D)J recombinatorial repertoire diversification during intraclonal pro-B to B-cell differentiation
    Yui Hsi Wang
    Division of Developmental and Clinical Immunology, and Department of Medicine, University of Alabama at Birmingham and the Howard Hughes Medical Institute, Birmingham, AL 35294, USA
    Blood 101:1030-7. 2003
    ..These findings validate the principle of intraclonal V(D)J diversification during B-cell generation and define a manipulable model of human B-cell differentiation...
  87. ncbi B cell development and proliferation of mature B cells in human fetal intestine
    Sarah Golby
    Department of Histopathology, GKT Medical School, St Thomas Campus, London, United Kingdom
    J Leukoc Biol 72:279-84. 2002
    ..Distinguishing characteristics of Ig lambda genes in postnatal gut were also studied in the fetus. According to these parameters, fetal and postnatal B cells are unrelated...
  88. ncbi Development of B cells in scid mice with immunoglobulin transgenes: implications for the control of V(D)J recombination
    Y Chang
    Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, Pennsylvania 19111, USA
    Immunity 2:607-16. 1995
    ..This rescue of scid B cell differentiation is associated with a dramatic reduction in expression of the recombination activation genes, RAG1 and RAG2, and with reduced transcription of the kappa locus...
  89. ncbi Features of the overexpressed V1-69 genes in the unmutated subset of chronic lymphocytic leukemia are distinct from those in the healthy elderly repertoire
    Kathleen N Potter
    Molecular Immunology Group, Tenovus Laboratory, Southampton University Hospitals Trust, Southampton, United Kingdom
    Blood 101:3082-4. 2003
    ..These findings support the concept that CLL arises from B cells driven by antigen/superantigen and is not a stochastic event in the elderly B-cell population...
  90. ncbi Role of receptor revision in forming a B cell repertoire
    Jane Seagal
    Department of Immunology, Bruce Rappaport Faculty of Medicine and Rappaport Family Institute for Research in the Medical Sciences, Technion Israel Institute of Technology, Haifa 31096, Israel
    Clin Immunol 105:1-8. 2002
  91. pmc Clues to the etiology of autoimmune diseases through analysis of immunoglobulin genes
    Jason M Link
    Department of Microbiology, Division of Developmental and Clinical Immunology, Wallace Tumor Institute 378, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham, AL 35294, USA
    Arthritis Res 4:80-3. 2002
    ....
  92. ncbi Salivary gland B cell lymphoproliferative disorders in Sjögren's syndrome present a restricted use of antigen receptor gene segments similar to those used by hepatitis C virus-associated non-Hodgkins's lymphomas
    Valli De Re
    Division of Experimental Oncology 1, Centro di Riferimento Oncologico, IRCS, Aviano, Italy
    Eur J Immunol 32:903-10. 2002
    ....
  93. ncbi Somatic immunoglobulin hypermutation
    Marilyn Diaz
    Department of Immunology, Imm16, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Curr Opin Immunol 14:235-40. 2002
    ..The nature of the targeting mechanism and the trans-factors effecting such breaks and their repair remain to be determined...
  94. ncbi Antibody repertoire development in fetal and neonatal piglets. VI. B cell lymphogenesis occurs at multiple sites with differences in the frequency of in-frame rearrangements
    Marek Sinkora
    Department of Immunology and Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Doly 183, 549 22 Novy Hradek, Czech Republic
    J Immunol 170:1781-8. 2003
    ..We propose the latter to result from differential selection or a developmentally dependent change in the intrinsic mechanism of VDJ rearrangement...
  95. ncbi Ligand-dependent and -independent processes in B-cell-receptor-mediated signaling
    E M Fuentes-Pananá
    University of Pennsylvania School of Medicine, Room 311 BRBII III, 421 Curie Blvd, Philadelphia, PA 19104, USA
    Springer Semin Immunopathol 23:333-50. 2001
    ..Here we discuss the evidence for ligand-independent functions for the BCR and postulate how it may be regulated and linked to biological processes associated with B cell development and survival...
  96. pmc Modifications of histone cores and tails in V(D)J recombination
    Kathrin Muegge
    Laboratory of Molecular Immunoregulation, SAIC Basic Science Program, National Cancer Institute, Frederick, MD 21701, USA
    Genome Biol 4:211. 2003
    ..A recent study shows that methylated lysine 79 in the core region of histone H3 also plays a role by providing a euchromatic 'mark' that may regulate access of the V(D)J recombinase...
  97. ncbi B cells in epithelial and perivascular compartments of human adult thymus
    K G Flores
    Department of Pathology and the Cell and Molecular Biology Program, Duke University Medical Center, Durham, NC 27710, USA
    Hum Pathol 32:926-34. 2001
    ....
  98. ncbi Epstein-Barr virus (EBV)-positive lymphoproliferations in post-transplant patients show immunoglobulin V gene mutation patterns suggesting interference of EBV with normal B cell differentiation processes
    Andreas Bräuninger
    Department of Pathology, University of Frankfurt, Germany
    Eur J Immunol 33:1593-602. 2003
    ....
  99. pmc The VH gene repertoire of splenic B cells and somatic hypermutation in systemic lupus erythematosus
    Nicola L W Fraser
    Division of Immunology, Infection and Inflammation, University of Glasgow, Western Infirmary, Glasgow, Scotland, UK
    Arthritis Res Ther 5:R114-21. 2003
    ..This is the first study of the splenic GC response in human SLE...
  100. ncbi [Primers for detecting gene rearrangement in different regions of immunoglobulin heavy chain genes and their application in diagnosis of paraffin-embedded lymphoma tissues]
    Zong Li Qi
    Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
    Nan Fang Yi Ke Da Xue Xue Bao 28:1964-7. 2008
    ....
  101. ncbi A six-year-old boy with a suspicious thyroid nodule: intrathyroidal thymic tissue
    Carlos Hernandez-Cassis
    Department of Medicine, Division of Endocrinology, Diabetes and Metabolism University of Miami Miller School of Medicine, Miami, Florida, USA
    Thyroid 18:377-80. 2008
    ..Ectopic intrathyroidal thymus tissue that forms mass lesions as a consequence of aberrant thymic migration during embryogenesis is rarely reported in the literature...

Research Grants57

  1. Assessment of BAFF as a mucosal HIV DNA vaccine adjuvant
    David Nemazee; Fiscal Year: 2005
    ....
  2. Immune Tolerance in Non-clonal Immune Systems
    David Nemazee; Fiscal Year: 2003
    ..These studies should have broad applications to questions of lymphocyte activation, differentiation, and survival. ..
  3. Manipulating the IgE response with IgE-reactive macroself antigen
    David Nemazee; Fiscal Year: 2007
    ..Furthermore, IgE macroself Ag-mediated suppression by gene transfer will be assessed both during and after antigenic sensitization. [unreadable] [unreadable]..
  4. Effect of BAFF on threshold of B cell tolerance
    David Nemazee; Fiscal Year: 2010
    ..The long-term goal of these studies is to understand how BAFF promotes Sjogren's Syndrome and to determine the basic parameters regulating immune tolerance. ..
  5. B LYMPHOCYTE TOLERANCE IN HEALTH AND AUTOIMMUNITY
    David Nemazee; Fiscal Year: 2009
    ....
  6. RECEPTOR EDITING IN B CELLS
    David Nemazee; Fiscal Year: 2010
    ..ARRA Response:2 R01 A1033608-16A1 ARRA Response: 2 ROl A1033608-16A1 F-- protein GSK3$3 is poorly activated by BCR signaling in immature B cells. L-' ..
  7. IMMUNOGLOBULIN V GENES AND B CELL DEVELOPMENT
    Richard Hardy; Fiscal Year: 2006
    ..The results of these studies will help to establish the types of selection that shape the expressed B cell repertoire, both fetally and in the adult, with implications for modulating normal and autoimmune responses. ..
  8. BD Biosciences LSR Flow Cytometer
    Richard Hardy; Fiscal Year: 2002
    ..abstract_text> ..
  9. Receptor Editing in Autoimmune and Normal Mice
    ANN FEENEY; Fiscal Year: 2009
    ..The information gained in these aims should give insights into the response of the various Ig loci to receptor editing signals, and how these processes may potentially be misregulated in B cells of autoimmune mice. ..
  10. THE ROLE OF COMPLEMENT IN AUTOIMMUNITY
    Garnett Kelsoe; Fiscal Year: 2005
    ..Thus, we propose that the innate immune system controls both the frequency of peripheral, self-reactive B cells and their exposure to antigen. ..
  11. RHEUMATIC DISEASES CORE CENTER
    Gregg Silverman; Fiscal Year: 2006
    ..An Administrative Core (PI-G.J. Silverman, Center Director) will provide overall direction and supervision to all Center programs through an Executive Committee, which will be assisted by an Outside Advisory Committee. ..
  12. B CELL REPERTOIRE FORMATION
    ANN FEENEY; Fiscal Year: 2009
    ..Together, these studies should give insight into the epigenetic mechanisms and cis-elements which control VH gene rearrangement in fetal and adult pro-B cells. ..
  13. B CELL SUPERANTIGENS AND IMMUNE REGULATION
    Gregg Silverman; Fiscal Year: 2008
    ..In addition, these studies will lead to a better understanding of the immunobiologic activities of a virulence factor from one of the most important causes of life-threatening infection in the US. ..
  14. Arthritis Research Conference 2005
    Gregg Silverman; Fiscal Year: 2005
    ..abstract_text> ..
  15. Arthritis Research Conference 2007
    Gregg Silverman; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  16. MOLECULAR ANALYSIS OF THE ANTIH INFLUENZAE RESPONSE
    ANN FEENEY; Fiscal Year: 2001
    ..We will also further analyze the diversity in the ani-hib response through the use of combinatorial libraries, correlating affinity with the Vh, Vk and CDR3's which are used. ..
  17. Cell Death and Antibody-Mediated Protection from Autoimmunity
    GREGG JOSHUA SILVERMAN; Fiscal Year: 2010
    ....
  18. ANALYSIS OF THE MURINE ANTIBODY REPERTOIRE
    GARNETT H KELSOE; Fiscal Year: 2010
    ..Qom cap mop axiom 0-c 0opccc woo'- t00 0co -td ..
  19. Mechanisms to Induce Protective Natural Autoimmunity
    Gregg Silverman; Fiscal Year: 2004
    ..abstract_text> ..
  20. alpha4beta1 Intergrin Signaling In Immunity And Arthritis
    GREGG JOSHUA SILVERMAN; Fiscal Year: 2010
    ..Collectively, this work will provide insight into the role of the ct4-paxillin interaction in inflammation and immunity, and whether this interaction is a potential bona fide therapeutic target for arthritis. ..
  21. Control of Accessibility for V(D) J Recombination
    ANN FEENEY; Fiscal Year: 2006
    ..We will determine if other transcription factors such as Pax5 or Btk will induce rearrangement of other Ig loci, such as Vh or lambda. ..
  22. Characterization of V(D)J cleavage and repair complexes
    PATRICK SWANSON; Fiscal Year: 2007
    ..abstract_text> ..
  23. The role of PKK in NFkappa B activation
    Shiv Pillai; Fiscal Year: 2007
    ..PKK represents a novel enzyme that may be critical in pathways that are important in cancer, inflammation, cell survival, and differentiation. ..
  24. Molecular basis of the requirements for IRF4 and IRF8 in pre-B cell development
    Runqing Lu; Fiscal Year: 2010
    ..In the absence of IRF4,8, pro-B cell development appears to be normal but pre-B cell development is blocked. We hypothesize that expression of IRF4,8 is induced at the pre-B stage by pre-BCR though Blnk/Btk-mediated signaling. ..
  25. Gene Repression in Fragile X Syndrome
    Howard Cedar; Fiscal Year: 2006
    ..abstract_text> ..
  26. The role of Immunoglobulin CDRH3 in Autoimmune Disease
    HARRY WILLIAM SCHROEDER; Fiscal Year: 2010
    ....
  27. Differential GVHD induction by naive and memory T cells
    Mark Shlomchik; Fiscal Year: 2009
    ..These studies can clearly help set the stage for applying this approach in rationally designed clinical pilot studies. ..
  28. DNA Breaks in Class Switch Recombination
    CAROL SCHRADER; Fiscal Year: 2009
    ..Enzymes from the BER pathway will be used to treat genomic DNA from B cells induced to switch in order to detect intermediates in the repair pathway that are predicted by this model. ..
  29. DNA REPLICATION IN ANIMAL CELLS
    Howard Cedar; Fiscal Year: 2009
    ....
  30. Molecular identification of a sialyl 9-O-acetyl transferase
    Shiv Pillai; Fiscal Year: 2008
    ..The gene that codes for an enzyme that controls the strength of the immune response will be studied. This gene may be an important target for the treatment of lupus and other related diseases. [unreadable] [unreadable] [unreadable]..
  31. IMMUNOREGULATION OF MURINE CD8+ T CELLS
    Pamela Fink; Fiscal Year: 2009
    ..abstract_text> ..
  32. ONTOGENY AND PHYLOGENY OF THE MHC
    Martin Flajnik; Fiscal Year: 2009
    ..tropicalis), we will begin to analyze the modes of silencing in the tetraploids and eventually the higher order octo- and dodecaploid individuals. ..
  33. MOUSE MODELS TO STUDY THE ROLE OF TRAF1 AND TRAF2 IN THE ETIOLOGY OF LEUKEMIA
    Juan Zapata; Fiscal Year: 2007
    ..Characterization of the CLL-like lymphoproliferative disorder caused by overexpression of TRAF2DN and Bcl-2 in B cells. [unreadable] [unreadable] [unreadable] [unreadable]..
  34. B LYMPHOCYTE INDUCED MATURATION PROTEIN
    Kathryn Calame; Fiscal Year: 2007
    ..We will purify Blimp-1-containing repression complexes and identify the components of the complexes. ..
  35. Pathogenesis of Notch Leukemia
    Jon Aster; Fiscal Year: 2007
    ..1. To identify domains of MAML1 that are essential for function in normal and transformed lymphoid cells 3.2. To identify and characterize small molecule inhibitors of CSL/ICN/MAML1 function ..
  36. Testing in Vivo Roles of TLRs in Systemic Autoimmunity
    Mark Shlomchik; Fiscal Year: 2004
    ..Because TLR9 is already strongly implicated in the activation of RF B cells in vitro and also by emerging data in vivo, we will focus on the role of this receptor on B cells. ..
  37. Highly Stable, Anthrax-specific Shark Antibody Fragment
    Martin Flajnik; Fiscal Year: 2004
    ....
  38. Marginal zone B lymphocytes and blood borne pathogens
    Shiv Pillai; Fiscal Year: 2004
    ..Finally we will attempt to determine whether antigen-specific MZ B cells, as opposed to follicuar B cells, can readily capture and present blood borne antigens, and thus activate na'fve T cells. ..
  39. TCRb CDR3 and Susceptibility to Autoimmune Disease
    Harry Schroeder; Fiscal Year: 2003
    ..These experiments will also shed light on the role of potentially abnormal TCR sequence in promoting lupus and other autoimmune diseases. ..
  40. Anti-Sm B-1 Cell Differentiation and Function
    Stephen Clarke; Fiscal Year: 2003
    ..In the final aim, the fate of B cells that normally differentiate to B-l will be determined in autoimmune MRL/lpr mice using a Tg model system of B-l differentiation. ..
  41. TRANSCRIPTION OF IMMUNOGLOBULIN HEAVY CHAIN GENES
    Kathryn Calame; Fiscal Year: 2001
    ....
  42. MoFlo High Speed Fluorescence Activated Cell Sorter
    Mark Shlomchik; Fiscal Year: 2003
    ..Twenty-four NIH-funded researchers would benefit from this instrument at YSM. Several young investigators not yet NIH-funded would also benefit. ..
  43. Pre-BCR expression level regulates cellular functions
    Stephen Clarke; Fiscal Year: 2002
    ..In the final aim whether the basis for the inability of 8/GO Tg mice to generate immature B cells is due to a pre-BCR or BCR deficiency will be determined. ..
  44. Activation and Regulation of Rheumatoid Factor B cells
    Mark Shlomchik; Fiscal Year: 2002
    ..abstract_text> ..
  45. DEVELOPMENT OF THE ANTIBODY REPERTOIRE
    Harry Schroeder; Fiscal Year: 2005
    ..Finally, the effect that alteration of HCDR3 has on the development of the natural autoantibody repertoire and on the progression of autoimmune disease in the MRL-lpr/lpr mouse will be examined. ..
  46. Studies on human peripheral B lymphocytes
    Shiv Pillai; Fiscal Year: 2005
    ....
  47. Analyzing VDJ recombination at the single molecule level
    David Roth; Fiscal Year: 2005
    ....
  48. LSRII Flow Cytometric Analyzer
    Mark Shlomchik; Fiscal Year: 2007
    ..of 27 NIH-funded PIs who require sophisticated FACS analysis and specific features of the LSRII, which otherwise may not be available due to limited capacity or capability of our existing machine [unreadable] [unreadable] [unreadable]..
  49. Anti-Sm B Cell Regulation
    Stephen Clarke; Fiscal Year: 2007
    ..In addition, we will test the hypothesis that the unusual pattern of CD 19 expression affects tolerance and activation. ..
  50. Targeting of somatic hypermutation and gene conversion
    David Schatz; Fiscal Year: 2006
    ..The results of these experiments should set the stage for significant progress toward an understanding of the mechanism by which protein factors and DNA sequences collaborate to target SM and GC to Ig genes. ..
  51. IMMUNOGLOBULIN AND T CELL RECEPTOR GENE ASSEMBLY
    David Schatz; Fiscal Year: 2006
    ..Overall, our experiments will address both the targeting and rejoining functions of the RAG proteins, with the long term goal of linking these fundamental activities to chromosomal translocations found in human malignancies. ..
  52. Role and Regulation of BLIMP-1 in Myeloid Cells
    Kathryn Calame; Fiscal Year: 2006
    ..Finally, the signals and mechanisms responsible for regulation of Blimp-1 expression in myeloid cells will be studied. ..
  53. TRANSCRIPTION AND MODIFICATION OF CHROMATIN DNA
    Howard Cedar; Fiscal Year: 2005
    ..abstract_text> ..
  54. MOUSE MODELS TO STUDY MULIBREY NANISM
    Juan Zapata; Fiscal Year: 2005
    ..study the developmental and phenotypic alterations of trim37 knock out and FINmajor-mutated trim37 mice. These mice will be the foundation for further studies on the biology and etiology of Mulibrey Nanism. ..
  55. PHENOTYPIC ANALYSES OF RECENT THYMIC EMIRGRANTS IN MICE
    Pamela Fink; Fiscal Year: 2005
    ..The overall goal of this work is to understand how thymic output and post thymic maturation vary with age, with an eye toward modulating immune senescence and recovery from lymphoablative therapies and diseases. ..
  56. Patterns of gene expression in Mulibrey Nanism
    Juan Zapata; Fiscal Year: 2005
    ..Specific Aim 2, is there any alteration in the patterns of gene expression induced by cytokines in normal and Mulibrey Nanism fibroblasts? Specific Aim 3, what is the role of TRIM37 in regulating gene expression? ..
  57. MEMBRANE IMMUNOGLOBULIN ASSEMBLY AND FUNCTION
    Shiv Pillai; Fiscal Year: 2002
    ..Since the majority of human lymphomas develop form activated B-cells, these signaling mechanisms are likely to be of direct relevance for a significant proportion of human cancers. ..