chromatin assembly and disassembly

Summary

Summary: The mechanisms effecting establishment, maintenance, and modification of that specific physical conformation of CHROMATIN determining the transcriptional accessibility or inaccessibility of the DNA.

Top Publications

  1. ncbi Chromatin modifications and their function
    Tony Kouzarides
    The Gurdon Institute and Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB21QN, UK
    Cell 128:693-705. 2007
  2. ncbi The biology of chromatin remodeling complexes
    Cedric R Clapier
    Howard Hughes Medical Institute, Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
    Annu Rev Biochem 78:273-304. 2009
  3. ncbi The role of chromatin during transcription
    Bing Li
    Stowers Medical Research Institute, 1000 East 50 th Street, Kansas City, MO 64110, USA
    Cell 128:707-19. 2007
  4. pmc Determinants of nucleosome organization in primary human cells
    Anton Valouev
    Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305, USA
    Nature 474:516-20. 2011
  5. ncbi Nuclear organization of active and inactive chromatin domains uncovered by chromosome conformation capture-on-chip (4C)
    Marieke Simonis
    Department of Cell Biology and Genetics, Erasmus Medical Centre, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    Nat Genet 38:1348-54. 2006
  6. ncbi SWI/SNF nucleosome remodellers and cancer
    BORIS G WILSON
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Division of Hematology Oncology, Children s Hospital Boston, Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Nat Rev Cancer 11:481-92. 2011
  7. pmc Genome-wide nucleosome specificity and directionality of chromatin remodelers
    Kuangyu Yen
    Department of Biochemistry and Molecular Biology, Center for Eukaryotic Gene Regulation, The Pennsylvania State University, University Park, PA 16802, USA
    Cell 149:1461-73. 2012
  8. pmc Daxx is an H3.3-specific histone chaperone and cooperates with ATRX in replication-independent chromatin assembly at telomeres
    Peter W Lewis
    Laboratory of Chromatin Biology and Epigenetics, Rockefeller University, 1230 York Ave, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:14075-80. 2010
  9. pmc FoxA1 translates epigenetic signatures into enhancer-driven lineage-specific transcription
    Mathieu Lupien
    Division of Molecular and Cellular Oncology, Department of Medical Oncology, Dana Farber Cancer Institute and Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Cell 132:958-70. 2008
  10. pmc Relationship between nucleosome positioning and DNA methylation
    Ramakrishna K Chodavarapu
    Department of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California 90095, USA
    Nature 466:388-92. 2010

Detail Information

Publications275 found, 100 shown here

  1. ncbi Chromatin modifications and their function
    Tony Kouzarides
    The Gurdon Institute and Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB21QN, UK
    Cell 128:693-705. 2007
    ..In this way, histone modifications have the potential to influence many fundamental biological processes, some of which may be epigenetically inherited...
  2. ncbi The biology of chromatin remodeling complexes
    Cedric R Clapier
    Howard Hughes Medical Institute, Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
    Annu Rev Biochem 78:273-304. 2009
    ..We also address roles for remodelers in development, cancer, and human syndromes...
  3. ncbi The role of chromatin during transcription
    Bing Li
    Stowers Medical Research Institute, 1000 East 50 th Street, Kansas City, MO 64110, USA
    Cell 128:707-19. 2007
    ..In this Review, we highlight advances in our understanding of chromatin regulation and discuss how such regulation affects the binding of transcription factors as well as the initiation and elongation steps of transcription...
  4. pmc Determinants of nucleosome organization in primary human cells
    Anton Valouev
    Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305, USA
    Nature 474:516-20. 2011
    ..Our results unveil an interplay of sequence-based nucleosome preferences and non-nucleosomal factors in determining nucleosome organization within mammalian cells...
  5. ncbi Nuclear organization of active and inactive chromatin domains uncovered by chromosome conformation capture-on-chip (4C)
    Marieke Simonis
    Department of Cell Biology and Genetics, Erasmus Medical Centre, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    Nat Genet 38:1348-54. 2006
    ..Our data demonstrate that chromosomes fold into areas of active chromatin and areas of inactive chromatin and establish 4C technology as a powerful tool to study nuclear architecture...
  6. ncbi SWI/SNF nucleosome remodellers and cancer
    BORIS G WILSON
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Division of Hematology Oncology, Children s Hospital Boston, Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Nat Rev Cancer 11:481-92. 2011
    ..In this Review we discuss the contributions of SWI/SNF mutations to cancer formation, examine their normal functions and discuss opportunities for novel therapeutic interventions for SWI/SNF-mutant cancers...
  7. pmc Genome-wide nucleosome specificity and directionality of chromatin remodelers
    Kuangyu Yen
    Department of Biochemistry and Molecular Biology, Center for Eukaryotic Gene Regulation, The Pennsylvania State University, University Park, PA 16802, USA
    Cell 149:1461-73. 2012
    ..Together, these findings reveal a coordinated position-specific approach taken by remodelers to organize genic nucleosomes into arrays...
  8. pmc Daxx is an H3.3-specific histone chaperone and cooperates with ATRX in replication-independent chromatin assembly at telomeres
    Peter W Lewis
    Laboratory of Chromatin Biology and Epigenetics, Rockefeller University, 1230 York Ave, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:14075-80. 2010
    ..These data demonstrate that Daxx functions as an H3.3-specific chaperone and facilitates the deposition of H3.3 at heterochromatin loci in the context of the ATRX-Daxx complex...
  9. pmc FoxA1 translates epigenetic signatures into enhancer-driven lineage-specific transcription
    Mathieu Lupien
    Division of Molecular and Cellular Oncology, Department of Medical Oncology, Dana Farber Cancer Institute and Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Cell 132:958-70. 2008
    ..FoxA1 translates this epigenetic signature into changes in chromatin structure thereby establishing lineage-specific transcriptional enhancers and programs...
  10. pmc Relationship between nucleosome positioning and DNA methylation
    Ramakrishna K Chodavarapu
    Department of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California 90095, USA
    Nature 466:388-92. 2010
    ..DNA methylation is also enriched on exons, consistent with the targeting of DNA methylation to nucleosomes, and suggesting a role for DNA methylation in exon definition...
  11. ncbi Frequent mutations of chromatin remodeling genes in transitional cell carcinoma of the bladder
    Yaoting Gui
    Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU HKUST Medical Center, Shenzhen, China
    Nat Genet 43:875-8. 2011
    ..Our results provide an overview of the genetic basis of TCC and suggest that aberration of chromatin regulation might be a hallmark of bladder cancer...
  12. ncbi Nucleosome positioning and gene regulation: advances through genomics
    Cizhong Jiang
    Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Nat Rev Genet 10:161-72. 2009
    ..A detailed picture is starting to emerge of how diverse factors, including underlying DNA sequences and chromatin remodelling complexes, influence nucleosome positioning...
  13. ncbi A packing mechanism for nucleosome organization reconstituted across a eukaryotic genome
    Zhenhai Zhang
    Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    Science 332:977-80. 2011
    ..Thus, an active, nonstatistical nucleosome packing mechanism creates chromatin organizing centers at the 5' ends of genes where important regulatory elements reside...
  14. pmc Chromatin remodelling during development
    Lena Ho
    Stanford University Medical School, Room B211, Beckman Center, 279 Campus Drive, Stanford, California 94305, USA
    Nature 463:474-84. 2010
    ..Particularly intriguing are the findings that specialized assemblies of ATP-dependent remodellers are essential for establishing and maintaining pluripotent and multipotent states in cells...
  15. pmc Chd1 chromatin remodelers maintain nucleosome organization and repress cryptic transcription
    Bianca P Hennig
    Biochemistry Center, BZH, Heidelberg University, Im Neuenheimer Feld 328, Heidelberg 69120, Germany
    EMBO Rep 13:997-1003. 2012
    ..These data indicate several mechanisms in the repression of cryptic promoter activity in eukaryotic cells...
  16. pmc Hyperdynamic plasticity of chromatin proteins in pluripotent embryonic stem cells
    Eran Meshorer
    National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Dev Cell 10:105-16. 2006
    ....
  17. ncbi The SWI/SNF complex and cancer
    D Reisman
    Department of Internal Medicine, University of Michigan College of Medicine, Ann Arbor, MI 48109 0686, USA
    Oncogene 28:1653-68. 2009
    ....
  18. pmc ATP-dependent chromatin remodeling: genetics, genomics and mechanisms
    Diana C Hargreaves
    Howard Hughes Medical Institute, Beckman Center B211, 279 Campus Drive, Mailcode 5323, Stanford University School of Medicine, Stanford, CA 94305 5323, USA
    Cell Res 21:396-420. 2011
    ..The mechanistic bases underlying the genetic requirements for BAF and other chromatin remodelers in development and cancer are relatively unexplored and will be a focus of this review...
  19. pmc A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization
    Triantaffyllos Gkikopoulos
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK
    Science 333:1758-60. 2011
    ..These observations indicate that adenosine triphosphate-dependent remodeling enzymes are responsible for directing the positioning of the majority of nucleosomes within the Saccharomyces cerevisiae genome...
  20. pmc An embryonic stem cell chromatin remodeling complex, esBAF, is essential for embryonic stem cell self-renewal and pluripotency
    Lena Ho
    Program in Immunology, Stanford University, Stanford, CA, USA
    Proc Natl Acad Sci U S A 106:5181-6. 2009
    ....
  21. pmc Chromatin dynamics during epigenetic reprogramming in the mouse germ line
    Petra Hajkova
    Wellcome Trust Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK
    Nature 452:877-81. 2008
    ....
  22. pmc Poly(ADP-ribose)-dependent regulation of DNA repair by the chromatin remodeling enzyme ALC1
    Dragana Ahel
    DNA Damage Response Laboratory, Clare Hall, London Research Institute, South Mimms EN6 3LD, UK
    Science 325:1240-3. 2009
    ..Furthermore, we show that depletion or overexpression of ALC1 results in sensitivity to DNA-damaging agents. Collectively, these results provide new insights into the mechanisms by which poly(ADP-ribose) regulates DNA repair...
  23. pmc In vivo effects of histone H3 depletion on nucleosome occupancy and position in Saccharomyces cerevisiae
    Andrea J Gossett
    Department of Biology, Carolina Center for Genome Sciences, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS Genet 8:e1002771. 2012
    ....
  24. pmc An essential switch in subunit composition of a chromatin remodeling complex during neural development
    Julie Lessard
    Howard Hughes Medical Institute and Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA
    Neuron 55:201-15. 2007
    ..More broadly, these studies suggest that SWI/SNF-like complexes in vertebrates achieve biological specificity by combinatorial assembly of their subunits...
  25. pmc ATRX interacts with H3.3 in maintaining telomere structural integrity in pluripotent embryonic stem cells
    Lee H Wong
    Chromosome and Chromatin Research, Murdoch Childrens Research Institute, Department of Paediatrics, University of Melbourne, Royal Children s Hospital, Parkville, Victoria 3052, Australia
    Genome Res 20:351-60. 2010
    ..These findings suggest a novel function of ATRX, working in conjunction with H3.3 and CBX5, as a key regulator of ES-cell telomere chromatin...
  26. pmc What controls nucleosome positions?
    Eran Segal
    Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot, 76100, Israel
    Trends Genet 25:335-43. 2009
    ....
  27. ncbi The logic of chromatin architecture and remodelling at promoters
    Bradley R Cairns
    Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    Nature 461:193-8. 2009
    ..Recent genome-wide studies in yeast suggest that constitutive and regulated genes have architectures that differ in terms of nucleosome position, turnover, remodelling requirements and transcriptional noise...
  28. pmc Remodelers organize cellular chromatin by counteracting intrinsic histone-DNA sequence preferences in a class-specific manner
    Yuri M Moshkin
    Department of Biochemistry and Center for Biomedical Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands
    Mol Cell Biol 32:675-88. 2012
    ..We conclude that the cellular nucleosome landscape is the result of the balance between DNA sequence-driven nucleosome placement and active nucleosome repositioning by remodelers and the transcription machinery...
  29. ncbi Gene silencing in X-chromosome inactivation: advances in understanding facultative heterochromatin formation
    Anton Wutz
    Wellcome Trust Centre for Stem Cell Research, Cambridge, UK
    Nat Rev Genet 12:542-53. 2011
    ..The emerging picture is complex and suggests that chromosome-wide silencing can be partitioned into several steps, the molecular components of which are starting to be defined...
  30. pmc Ring1B compacts chromatin structure and represses gene expression independent of histone ubiquitination
    Ragnhild Eskeland
    MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Crewe Road, Edinburgh EH4 2XU, UK
    Mol Cell 38:452-64. 2010
    ..We suggest that Ring1B-mediated chromatin compaction acts to directly limit transcription in vivo...
  31. pmc Understanding the words of chromatin regulation
    Jiang I Wu
    Howard Hughes Medical Institute, Departments of Pathology and Developmental Biology, Stanford University, Stanford, CA 94062, USA
    Cell 136:200-6. 2009
    ..Combinatorial assembly of chromatin regulatory complexes may be critical for maximizing the information content provided by arrays of histone modifications...
  32. pmc Cancer biology and NuRD: a multifaceted chromatin remodelling complex
    Anne Y Lai
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina NC 27709, USA
    Nat Rev Cancer 11:588-96. 2011
    ....
  33. ncbi Histone H4-K16 acetylation controls chromatin structure and protein interactions
    Michael Shogren-Knaak
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 311:844-7. 2006
    ....
  34. pmc Distinct roles for SWR1 and INO80 chromatin remodeling complexes at chromosomal double-strand breaks
    Haico van Attikum
    Division of Epigenetics, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
    EMBO J 26:4113-25. 2007
    ..Thus, these two related chromatin remodelers have distinct roles in DSB repair and checkpoint activation...
  35. pmc esBAF facilitates pluripotency by conditioning the genome for LIF/STAT3 signalling and by regulating polycomb function
    Lena Ho
    Program in Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
    Nat Cell Biol 13:903-13. 2011
    ..Therefore, esBAF does not simply antagonize PcG. Rather, the two chromatin regulators act both antagonistically and synergistically with the common goal of supporting pluripotency...
  36. pmc Poly(ADP-ribosyl)ation directs recruitment and activation of an ATP-dependent chromatin remodeler
    Aaron J Gottschalk
    The Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Proc Natl Acad Sci U S A 106:13770-4. 2009
    ..We propose that poly(ADP-ribosyl)ation of chromatin-associated Parp1 serves as a mechanism for targeting a SNF2 family remodeler to chromatin...
  37. pmc Nucleosome positioning: how is it established, and why does it matter?
    Marta Radman-Livaja
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Dev Biol 339:258-66. 2010
    ....
  38. ncbi The mammalian epigenome
    Bradley E Bernstein
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Cell 128:669-81. 2007
    ..Here we review current research efforts, with an emphasis on large-scale studies, emerging technologies, and challenges ahead...
  39. pmc ES cell pluripotency and germ-layer formation require the SWI/SNF chromatin remodeling component BAF250a
    Xiaolin Gao
    Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Richard Simches Research Center, 185 Cambridge Street, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 105:6656-61. 2008
    ..Our results suggest that BAF250a is a key component of the gene regulatory machinery in ES cells controlling self-renewal, differentiation, and cell lineage decisions...
  40. pmc Chaperoning histones during DNA replication and repair
    Monica Ransom
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045, USA
    Cell 140:183-95. 2010
    ..Conversely, the DNA has to be repackaged into chromatin afterward. This review focuses on the roles of the histone chaperones in assembling and disassembling chromatin during the processes of DNA replication and repair...
  41. ncbi Baf60c is essential for function of BAF chromatin remodelling complexes in heart development
    Heiko Lickert
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Nature 432:107-12. 2004
    ....
  42. pmc Histone H3 tail acetylation modulates ATP-dependent remodeling through multiple mechanisms
    Nilanjana Chatterjee
    Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL 62901 4413, USA
    Nucleic Acids Res 39:8378-91. 2011
    ..These data illustrate how histone acetylation modulates RSC and SWI/SNF function, and provide a mechanistic insight into their collaborative efforts to remodel chromatin...
  43. pmc Snf2-family proteins: chromatin remodellers for any occasion
    Daniel P Ryan
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee, DD1 5EH, UK
    Curr Opin Chem Biol 15:649-56. 2011
    ..Recent developments include new insights into the mechanism for nucleosome spacing and histone dimer exchange; together with growing evidence for the involvement of Snf2 proteins in DNA repair...
  44. pmc Global regulation of H2A.Z localization by the INO80 chromatin-remodeling enzyme is essential for genome integrity
    Manolis Papamichos-Chronakis
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Cell 144:200-13. 2011
    ..Z/H2B with free H2A/H2B dimers. Genetic interactions between ino80 and htz1 support a model in which INO80 catalyzes the removal of unacetylated H2A.Z from chromatin as a mechanism to promote genome stability...
  45. pmc Diversity of operation in ATP-dependent chromatin remodelers
    Swetansu K Hota
    Department of Biochemistry and Biology, Southern Illinois University School of Medicine, Carbondale, IL 62901 4413, USA
    Biochim Biophys Acta 1809:476-87. 2011
    ..This article is part of a Special Issue entitled: Snf2/Swi2 ATPase structure and function...
  46. pmc The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4
    Aleksandra A Watson
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK
    J Mol Biol 422:3-17. 2012
    ....
  47. ncbi Chromatin remodelling in mammalian cells by ISWI-type complexes--where, when and why?
    Fabian Erdel
    Research Group Genome Organization and Function, Deutsches Krebsforschungszentrum DKFZ and BioQuant, Heidelberg, Germany
    FEBS J 278:3608-18. 2011
    ..Here, we review the cellular activities of the mammalian imitation switch proteins and discuss mechanisms by which they are targeted to sites where their activity is needed...
  48. pmc Selective removal of promoter nucleosomes by the RSC chromatin-remodeling complex
    Yahli Lorch
    Department of Structural Biology, Stanford University School of Medicine, Stanford, California, USA
    Nat Struct Mol Biol 18:881-5. 2011
    ..These findings point to a principle of promoter chromatin remodeling for transcription, namely that promoter specificity resides primarily in the nucleosomes rather than in the remodeling complex that acts upon them...
  49. pmc The Saccharomyces cerevisiae chromatin remodeler Fun30 regulates DNA end resection and checkpoint deactivation
    Vinay V Eapen
    Department of Biology, Rosenstiel Basic Medical Sciences Center, Brandeis University, Waltham, Massachusetts, USA
    Mol Cell Biol 32:4727-40. 2012
    ..Fun30 is not required for later steps in homologous recombination. Like its homolog Rdh54/Tid1, Fun30 is required to allow the adaptation of DNA damage checkpoint-arrested cells with an unrepaired DSB to resume cell cycle progression...
  50. pmc Mechanism of chromatin remodeling and recovery during passage of RNA polymerase II
    Olga I Kulaeva
    Department of Pharmacology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey, USA
    Nat Struct Mol Biol 16:1272-8. 2009
    ..This coupling is a distinct feature of the Pol II-type mechanism that allows further transcription through the nucleosome, prevents nucleosome translocation and minimizes displacement of H3 and H4 histones from DNA during enzyme passage...
  51. ncbi Continuous histone H2B and transcription-dependent histone H3 exchange in yeast cells outside of replication
    Adil Jamai
    Department of Microbiology and Molecular Medicine, University Medical Centre, Rue Michel Servet 1, 1211 Geneva 4, Switzerland
    Mol Cell 25:345-55. 2007
    ....
  52. ncbi Polycomb group proteins Ezh2 and Rnf2 direct genomic contraction and imprinted repression in early mouse embryos
    Rémi Terranova
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH 4058 Basel, Switzerland
    Dev Cell 15:668-79. 2008
    ..We propose that the formation of a parental-specific higher-order chromatin organization renders imprint clusters competent for monoallelic silencing and assign a central role to PcG proteins in this process...
  53. pmc The histone shuffle: histone chaperones in an energetic dance
    Chandrima Das
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045, USA
    Trends Biochem Sci 35:476-89. 2010
    ....
  54. pmc UBF levels determine the number of active ribosomal RNA genes in mammals
    Elaine Sanij
    Research Division, Peter MacCallum Cancer Centre, East Melbourne, Victoria 3002, Australia
    J Cell Biol 183:1259-74. 2008
    ..We also show that the active rRNA gene pool is not static but decreases during differentiation, correlating with diminished UBF expression. Thus, UBF1 levels regulate active rRNA gene chromatin during growth and differentiation...
  55. pmc Histone chaperone FACT coordinates nucleosome interaction through multiple synergistic binding events
    Duane D Winkler
    Howard Hughes Medical Institute and the Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523 1870, USA
    J Biol Chem 286:41883-92. 2011
    ..Together, the data reveal that specific FACT subunits synchronize interactions with various target sites on individual nucleosomes to generate a high affinity binding event and promote reorganization...
  56. pmc ARID1A, a factor that promotes formation of SWI/SNF-mediated chromatin remodeling, is a tumor suppressor in gynecologic cancers
    Bin Guan
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Cancer Res 71:6718-27. 2011
    ..Our results provide functional evidence in support of the hypothesis that ARID1A is a bona fide tumor suppressor that collaborates with p53 to regulate CDKN1A and SMAD3 transcription and tumor growth in gynecologic cancers...
  57. ncbi Epigenetics, DNA methylation, and chromatin modifying drugs
    Moshe Szyf
    Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec H3G 1Y6, Canada
    Annu Rev Pharmacol Toxicol 49:243-63. 2009
    ..Understanding the epigenetic machinery and the differential roles of its components in specific disease states is essential for developing targeted epigenetic therapy...
  58. pmc The Chd family of chromatin remodelers
    Concetta G A Marfella
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Mutat Res 618:30-40. 2007
    ..Genetic, biochemical, and structural studies demonstrate that Chd proteins are important regulators of transcription and play critical roles during developmental processes. Numerous Chd proteins are also implicated in human disease...
  59. ncbi The histone methyltransferase MLL1 permits the oscillation of circadian gene expression
    Sayako Katada
    Unite 904 INSERM Epigenetic Control and Neuronal Plasticity, Department of Pharmacology, School of Medicine, University of California, Irvine, Irvine, California, USA
    Nat Struct Mol Biol 17:1414-21. 2010
    ..Our results favor a scenario in which H3K4 trimethylation by MLL1 is required to establish a permissive chromatin state for circadian transcription...
  60. ncbi Nuclear re-organisation of the Hoxb complex during mouse embryonic development
    Séverine Chambeyron
    MRC Human Genetics Unit, Crewe Road, Edinburgh EH4 2XU, UK
    Development 132:2215-23. 2005
    ..In early development nuclear re-organisation is coupled to Hoxb expression, but does not anticipate it...
  61. pmc WSTF regulates the H2A.X DNA damage response via a novel tyrosine kinase activity
    Andrew Xiao
    Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10065, USA
    Nature 457:57-62. 2009
    ..Our work demonstrates a new mechanism that regulates the DNA damage response and expands our knowledge of domains that contain intrinsic tyrosine kinase activity...
  62. pmc Interaction of transcriptional regulators with specific nucleosomes across the Saccharomyces genome
    R Thomas Koerber
    Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    Mol Cell 35:889-902. 2009
    ..These findings suggest that nucleosomes around promoter regions have position-specific functions and that some gene regulators have position-specific nucleosomal interactions...
  63. pmc Isolation of an activator-dependent, promoter-specific chromatin remodeling factor
    Andreas H Ehrensberger
    Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 108:10115-20. 2011
    ..Deletion of the CHD1 gene in an isw1Δ pho80Δ strain abolished PHO5 gene expression, demonstrating the relevance of the remodeling reaction in vitro to the process in vivo...
  64. ncbi Heterochromatin revisited
    Shiv I S Grewal
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Rev Genet 8:35-46. 2007
    ....
  65. ncbi Stepwise histone replacement by SWR1 requires dual activation with histone H2A.Z and canonical nucleosome
    Ed Luk
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 143:725-36. 2010
    ..Z-H2B. These results suggest that the combination of H2A-containing nucleosome and free H2A.Z-H2B dimer acting as both effector and substrate for SWR1 governs the specificity and outcome of the replacement reaction...
  66. pmc Concerted action of the PHD, chromo and motor domains regulates the human chromatin remodelling ATPase CHD4
    Rosa Morra
    Division of Structural Biology, Wellcome Trust Centre for Human Genetics, Oxford University, Roosevelt Drive, Oxford OX3 7BN, United Kingdom
    FEBS Lett 586:2513-21. 2012
    ..Our results demonstrate functional interdependency between domains within a chromatin remodeller...
  67. pmc TRIM24 links a non-canonical histone signature to breast cancer
    Wen Wei Tsai
    Department of Biochemistry and Molecular Biology, Program in Genes and Development, Graduate School of Biomedical Sciences, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Nature 468:927-32. 2010
    ..The PHD-Bromo of TRIM24 provides a structural rationale for chromatin activation through a non-canonical histone signature, establishing a new route by which chromatin readers may influence cancer pathogenesis...
  68. pmc Extranucleosomal DNA binding directs nucleosome sliding by Chd1
    Jeffrey N McKnight
    TC Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218 2685, USA
    Mol Cell Biol 31:4746-59. 2011
    ..These results suggest that the DNA-binding domain's affinity for extranucleosomal DNA is the key determinant for the direction that Chd1 shifts the nucleosome...
  69. pmc Maternal BRG1 regulates zygotic genome activation in the mouse
    Scott J Bultman
    Department of Genetics and the Carolina Center for Genome Sciences, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Genes Dev 20:1744-54. 2006
    ..Depletion of maternal BRG1 did not affect global levels of histone acetylation, whereas dimethyl-H3K4 levels were reduced. These data provide a framework for understanding the mechanism of ZGA...
  70. pmc Overlapping regulation of CenH3 localization and histone H3 turnover by CAF-1 and HIR proteins in Saccharomyces cerevisiae
    Jessica Lopes da Rosa
    Department of Molecular and Cell Biology, University of California, Berkeley, California 947202, USA
    Genetics 187:9-19. 2011
    ..We discuss ways in which altered histone exchange kinetics may affect eviction of Cse4 from noncentromeric loci...
  71. pmc Drosophila ISWI regulates the association of histone H1 with interphase chromosomes in vivo
    Giorgia Siriaco
    Adolf Butenandt Institute, Molecular Biology, Munich Center of Integrated Protein Science, Ludwig Maximilians University, 80336 Munich, Germany
    Genetics 182:661-9. 2009
    ..Our findings suggest that ISWI regulates higher-order chromatin structure by modulating the interaction of H1 with interphase chromosomes...
  72. pmc Distinct modes of regulation by chromatin encoded through nucleosome positioning signals
    Yair Field
    Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot, Israel
    PLoS Comput Biol 4:e1000216. 2008
    ....
  73. ncbi Chromatin replication and epigenome maintenance
    Constance Alabert
    Biotech Research and Innovation Centre, University of Copenhagen, Ole Maaløes Vej 5, DK2200 Copenhagen, Denmark
    Nat Rev Mol Cell Biol 13:153-67. 2012
    ..If DNA synthesis is perturbed, cells can suffer loss of both genome and epigenome integrity with severe consequences for the organism...
  74. pmc The DNA-binding domain of the Chd1 chromatin-remodelling enzyme contains SANT and SLIDE domains
    Daniel P Ryan
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, UK
    EMBO J 30:2596-609. 2011
    ..Through inclusion of Chd1 sequences in homology searches SLIDE domains were identified in CHD6-9 proteins. Point mutations to conserved amino acids within the human CHD7 SLIDE domain have been identified in patients with CHARGE syndrome...
  75. ncbi Multivalent engagement of chromatin modifications by linked binding modules
    Alexander J Ruthenburg
    Laboratory of Chromatin Biology, The Rockefeller University, New York, NY 10065, USA
    Nat Rev Mol Cell Biol 8:983-94. 2007
    ..We propose that multivalent interactions on a single histone tail and beyond may have a significant, if not dominant, role in chromatin transactions...
  76. ncbi The epigenetics of rRNA genes: from molecular to chromosome biology
    Brian McStay
    Biomedical Research Center, Ninewells Hospital, University of Dundee, Dundee DD1 9SY, United Kingdom
    Annu Rev Cell Dev Biol 24:131-57. 2008
    ..We also review the critical role of the RNA polymerase I transcription factor UBF in the formation of active nucleolar organizer regions (NORs) and maintenance of the euchromatic state of rRNA genes...
  77. pmc Remosomes: RSC generated non-mobilized particles with approximately 180 bp DNA loosely associated with the histone octamer
    Manu Shubhdarshan Shukla
    Universite Joseph Fourier Grenoble 1, Institut National de la Sante et de la Recherche Medicale, Institut Albert Bonniot, U823, Site Santé BP 170, 38042 Grenoble Cedex 9, France
    Proc Natl Acad Sci U S A 107:1936-41. 2010
    ..In agreement with this model, we show experimentally that the remosomes are intermediate products generated during the first step of the remodeling reaction that are further efficiently mobilized by RSC...
  78. ncbi Structure and mechanism of the chromatin remodelling factor ISW1a
    Kazuhiro Yamada
    ETH Zurich, Institute of Molecular Biology and Biophysics, Schafmattstr 20, CH 8093 Zurich, Switzerland
    Nature 472:448-53. 2011
    ..Results from a remodelling assay corroborate the dinucleosome model. We show how a chromatin remodelling factor could set the spacing between two adjacent nucleosomes acting as a 'protein ruler'...
  79. ncbi The Williams syndrome transcription factor interacts with PCNA to target chromatin remodelling by ISWI to replication foci
    Raymond A Poot
    Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, UK
    Nat Cell Biol 6:1236-44. 2004
    ..We propose that the WSTF-ISWI complex has a role in the maintenance of chromatin structures during DNA replication...
  80. pmc SHH1, a homeodomain protein required for DNA methylation, as well as RDR2, RDM4, and chromatin remodeling factors, associate with RNA polymerase IV
    Julie A Law
    Department of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS Genet 7:e1002195. 2011
    ....
  81. pmc Drosophila Kismet regulates histone H3 lysine 27 methylation and early elongation by RNA polymerase II
    Shrividhya Srinivasan
    Department of Molecular, Cell, and Developmental Biology, University of California Santa Cruz, Santa Cruz, CA, USA
    PLoS Genet 4:e1000217. 2008
    ..Our findings suggest that KIS-L promotes early elongation and counteracts Polycomb group repression by recruiting the ASH1 and TRX histone methyltransferases to chromatin...
  82. ncbi Pbx marks genes for activation by MyoD indicating a role for a homeodomain protein in establishing myogenic potential
    Charlotte A Berkes
    Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Mol Cell 14:465-77. 2004
    ..This demonstrates a specific mechanism of targeting MyoD to loci in inactive chromatin and reveals a critical role of homeodomain proteins in marking specific genes for activation in the muscle lineage...
  83. ncbi Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis in Drosophila
    Christina Rathke
    Philipps Universitat Marburg, Fachbereich Biologie, Entwicklungsbiologie, 35043 Marburg, Germany
    J Cell Sci 120:1689-700. 2007
    ..Finally, we show that histone modifications and removal are independent of protamine synthesis...
  84. pmc The histone chaperone FACT: structural insights and mechanisms for nucleosome reorganization
    Duane D Winkler
    Department of Biochemistry and Molecular Biology and the Howard Hughes Medical Institute, Colorado State University, Fort Collins, Colorado 80523 1870, USA
    J Biol Chem 286:18369-74. 2011
    ..Here, we review the structural and biophysical basis for FACT-mediated nucleosome reorganization and discuss up-to-date models for FACT function...
  85. pmc Developmental regulation and individual differences of neuronal H3K4me3 epigenomes in the prefrontal cortex
    Iris Cheung
    Department of Psychiatry, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Medical School, Worcester, MA 01604, USA
    Proc Natl Acad Sci U S A 107:8824-9. 2010
    ....
  86. ncbi Histone chaperone spt16 promotes redeposition of the original h3-h4 histones evicted by elongating RNA polymerase
    Adil Jamai
    Department of Microbiology and Molecular Medicine, University Medical Centre, Geneva, Switzerland
    Mol Cell 35:377-83. 2009
    ....
  87. pmc Distinct roles for the RSC and Swi/Snf ATP-dependent chromatin remodelers in DNA double-strand break repair
    Bob Chai
    Department of Microbiology and Immunology and Morse Institute for Molecular Genetics, State University of New York Downstate Medical Center, Brooklyn, New York 11203, USA
    Genes Dev 19:1656-61. 2005
    ..We show that Swi/Snf is required earlier, at or preceding the strand invasion step of HR, while RSC is required following synapsis for completion of the recombinational repair event...
  88. pmc Chd1 regulates open chromatin and pluripotency of embryonic stem cells
    Alexandre Gaspar-Maia
    Department of Ob Gyn and Pathology, Center for Reproductive Sciences and Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, California 94143 0525, USA
    Nature 460:863-8. 2009
    ..Our results indicate that Chd1 is essential for open chromatin and pluripotency of embryonic stem cells, and for somatic cell reprogramming to the pluripotent state...
  89. pmc Widespread remodeling of mid-coding sequence nucleosomes by Isw1
    Itay Tirosh
    Department of Molecular Genetics, Weizmann Institute of Science, Herzl Street, Rehovot 76100, Israel
    Genome Biol 11:R49. 2010
    ..While the genome-wide effect of most remodelers has not been described, recent studies in Saccharomyces cerevisiae have shown that Isw2 prevents ectopic expression of anti-sense and suppressed transcripts at gene ends...
  90. pmc A cooperative activation loop among SWI/SNF, gamma-H2AX and H3 acetylation for DNA double-strand break repair
    Han Sae Lee
    Division of Life and Pharmaceutical Sciences, Department of Life Science, Ewha Womans University, Seoul, Republic of Korea
    EMBO J 29:1434-45. 2010
    ..The H3 acetylation on gamma-H2AX nucleosomes is induced by DNA damage. These results collectively suggest that SWI/SNF, gamma-H2AX and H3 acetylation cooperatively act in a feedback activation loop to facilitate DSB repair...
  91. pmc Functional coupling between HIV-1 integrase and the SWI/SNF chromatin remodeling complex for efficient in vitro integration into stable nucleosomes
    Paul Lesbats
    Laboratoire MCMP, UMR 5234 CNRS Université Victor Segalen Bordeaux 2, Bordeaux, France
    PLoS Pathog 7:e1001280. 2011
    ..Our data indicate that some chromatin structures can be refractory for integration and that coupling between nucleosome remodeling and HIV-1 integration is required to overcome this natural barrier...
  92. ncbi Heterozygous missense mutations in SMARCA2 cause Nicolaides-Baraitser syndrome
    Jeroen K J Van Houdt
    Center for Human Genetics, Catholic University Leuven, University Hospital Gasthuisberg, Leuven, Belgium
    Nat Genet 44:445-9, S1. 2012
    ..These alterations likely do not impair SWI/SNF complex assembly but may be associated with disrupted ATPase activity. The identification of SMARCA2 mutations in humans provides insight into the function of the Snf2 helicase family...
  93. ncbi Loss of BAF250a (ARID1A) is frequent in high-grade endometrial carcinomas
    Kimberly C Wiegand
    The Center for Translational and Applied Genomics CTAG at the British Columbia BC Cancer Agency, Vancouver, BC, Canada
    J Pathol 224:328-33. 2011
    ....
  94. pmc A barcode screen for epigenetic regulators reveals a role for the NuB4/HAT-B histone acetyltransferase complex in histone turnover
    Kitty F Verzijlbergen
    Department of Gene Regulation, Netherlands Cancer Institute, Amsterdam, The Netherlands
    PLoS Genet 7:e1002284. 2011
    ..Thus, systematic and direct interrogation of chromatin structure on DNA barcodes can lead to the discovery of genes and pathways involved in chromatin modification and dynamics...
  95. ncbi Cocaine self-administration alters the expression of chromatin-remodelling proteins; modulation by histone deacetylase inhibition
    Lionel Host
    INSERM, U575, Centre de Neurochimie, Universite de Strasbourg, Strasbourg, France
    J Psychopharmacol 25:222-9. 2011
    ..The two factors known to regulate gene transcription are likely to play a role in the neurobiological mechanism underlying a decrease in the reinforcing properties of cocaine...
  96. ncbi Chromatin remodelling in mammalian differentiation: lessons from ATP-dependent remodellers
    Ivana L de la Serna
    Department of Biochemistry and Cancer Biology, Medical University of Ohio, 3035 Arlington Avenue, Toledo, Ohio 43606, USA
    Nat Rev Genet 7:461-73. 2006
    ....
  97. ncbi Strategies for the reconstitution of chromatin
    Alexandra Lusser
    Section of Molecular Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0347, USA
    Nat Methods 1:19-26. 2004
    ..Fortunately, there are a variety of methods by which a nonspecialist can prepare chromatin of high quality. Here, we describe strategies and techniques for the reconstitution of chromatin in vitro...
  98. pmc CenH3/CID incorporation is not dependent on the chromatin assembly factor CHD1 in Drosophila
    Valerie Podhraski
    Division of Molecular Biology, Biocenter, Innsbruck Medical University, Innsbruck, Austria
    PLoS ONE 5:e10120. 2010
    ..Therefore, centromeric chromatin assembly may involve different mechanisms in different organisms...
  99. pmc The role of epigenetics in the pathology of diabetic complications
    Louisa M Villeneuve
    Department of Diabetes, Beckman Research Institute of City of Hope, Duarte, California 91010, USA
    Am J Physiol Renal Physiol 299:F14-25. 2010
    ....
  100. pmc How eukaryotic genes are transcribed
    Bryan J Venters
    Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    Crit Rev Biochem Mol Biol 44:117-41. 2009
    ..The wealth of new insights arising from the tools of biochemistry, genomics, cell biology, and genetics is providing a remarkable view into the mechanics of gene regulation...
  101. pmc MacroH2A allows ATP-dependent chromatin remodeling by SWI/SNF and ACF complexes but specifically reduces recruitment of SWI/SNF
    Evelyn Y Chang
    Department of Biochemistry and Biophysics, University of California, San Francisco, California 94158, USA
    Biochemistry 47:13726-32. 2008
    ..Thus, macroH2A may help regulate the balance between activating and repressive remodeling complexes...

Research Grants62

  1. Macromolecular Crystallography Data Collection Instrumen
    Hwai Chen Guo; Fiscal Year: 2002
    ..abstract_text> ..
  2. Evolutionary Analysis of the Histone Deacetylase Family: Biomedical Research
    JOSEPH LEWIS GRAVES; Fiscal Year: 2011
    ..This in turn has the potential to inform drug development efforts impacting a breadth of specific diseases, as well as general aging itself. ..
  3. Dynamics of Transcriptional Regulation
    Elena Zelin; Fiscal Year: 2011
    ..Hence, investigation of molecular mechanisms of transcriptional control will potentially facilitate design of drug-and gene-based therapeutic strategies to treat these abnormalities. ..
  4. Regulation of repetitive chromatin structures during the human cell cycle
    Paul D Kaufman; Fiscal Year: 2013
    ..Together, these studies will provide candidate direct targets of p150 regulation, and lead us to test dependency relationships for these observations. ..
  5. Structural and Mechanistic Characterization of the Histone Chaperone FACT
    Duane David Winkler; Fiscal Year: 2012
    ..Insights from these proposed aims could aid in the development of specific FACT inhibitors that have the potential to increase chemotherapy effectiveness while decreasing aberrant cancer-related processes. ..
  6. Var gene regulation and antigenic variation in malaria
    Kirk W Deitsch; Fiscal Year: 2013
    ..The long-term goals are to develop methods to disrupt the process of antigenic variation and thereby shorten the length of an infection and reduce its severity. ..
  7. Histone chaperones and epigenetic silencing
    Zhiguo Zhang; Fiscal Year: 2013
    ....
  8. Biochemistry and genetics of chromatin assembly factors in Drosophila
    Dmitry V Fyodorov; Fiscal Year: 2013
    ..The goal of this project is to understand the function of factors that mediate the assembly of chromatin. Our work will help to design methods of diagnosis and treatment of human diseases that involve defects in nuclear reactions. ..
  9. Suppression of translocations by RecQ-like DNA helicases
    KRISTINA HILDEGARD SCHMIDT; Fiscal Year: 2012
    ..abstract_text> ..
  10. Chromatin Assembly Structure and Function
    Jessica K Tyler; Fiscal Year: 2010
    ..b>Chromatin assembly and disassembly are essential for duplication of the genome, yet are poorly understood...
  11. Mechanisms of the 3'-->5'deoxyribonucleases
    Fred W Perrino; Fiscal Year: 2012
    ..The outcome of the proposed research will have a significant impact on the medical treatment of complex autoimmune diseases such as systemic lupus erythematosus. ..
  12. Centromere identity and function
    Ben E Black; Fiscal Year: 2013
    ....
  13. Molecular Mechanism of Histone H3/H4 Chaperone Function
    MAIR E CHURCHILL; Fiscal Year: 2010
    ..Furthermore, chromatin assembly and disassembly processes are important in human disease, as seen in the multiple genetic malformations, as well as ..
  14. Type B Histone Acetyltransferases and the Assembly of Chromatin Structure
    Mark R Parthun; Fiscal Year: 2013
    ..This proposal seeks to understand the mechanisms by which chromatin is assembled and regulated which will aid in our understanding and treatment of these diseases. ..
  15. HISTONE NUCLEAR IMPORT AND CHROMATIN ASSEMBLY
    Lucy F Pemberton; Fiscal Year: 2010
    ..This highlights the importance of dissecting the function of histone chaperones in yeast as a model organism, and will give us useful information on the role of histone chaperones in human physiology and human disease. ..
  16. Modeling Chromatin Organization and Function
    Tamar Schlick; Fiscal Year: 2012
    ..Ultimately, this work's results have practical applications by impacting design of agents that control nucleosome composition and chromatin folds. ..
  17. Okazaki Fragment Synthesis and Chromatin Assembly
    Iestyn Whitehouse; Fiscal Year: 2013
    ..These studies will be directly relevant to may research programs and will translate into a better understanding of the biological processes fundamental to maintaining genomic integrity and chromatin states. ..
  18. Peptide Arrays for Understanding Histone Biochemistry
    Milan Mrksich; Fiscal Year: 2011
    ....
  19. ROLE OF CELLULAR PROTEINS IN HPV TRANSCRIPTION
    Cheng Ming Chiang; Fiscal Year: 2004
    ..These studies will uncover the role of cellular proteins in mediating E2 functions and further shed light on the molecular mechanism of HPV transcription. ..
  20. Anatomy of replication control elements in trypanosomes
    PRADEEP PATNAIK; Fiscal Year: 2002
    ..abstract_text> ..
  21. Factor X: A Cardiac-Specific Transcriptional Regulator
    Charles Ordahl; Fiscal Year: 2006
    ..abstract_text> ..
  22. Methyltransferase Drug Discovery
    Haiching Ma; Fiscal Year: 2009
    ..Based on these skills, RBC seeks to develop a new assay platform using gold standard radioisotope assays for the large family of histone lysine methyltransferases. ..
  23. REGULATION OF IMMUNOGLOBULIN GENE EXPRESSION IN B CELLS
    Ruibao Ren; Fiscal Year: 2003
    ..The rules of combinatorial transcription activation will also allow the design of novel transcription regulatory sequences to direct therapeutic expression of exogenous genes in selected cells. ..
  24. STRUCTURE OF INTERPHASE AND METAPHASE CHROMOSOMES
    MARTIN GOROVSKY; Fiscal Year: 1990
    ..These studies should illuminate the mechanisms which govern gene expression in eucaryotes...
  25. AN IVEM FOR HIGH RESOLUTION ELECTRONS CRYO-MICROSCOPY
    CHRISTOPHER AKEY; Fiscal Year: 2000
    ..abstract_text> ..
  26. Function of Genes in Williams Syndrome Deletion Region
    Uta Francke; Fiscal Year: 2005
    ....
  27. Assembly and Function of Human Centromere Chromatin
    Kevin Sullivan; Fiscal Year: 2004
    ....
  28. CHROMATIN STRUCTURE AND GENE ACTIVITY
    JAMES KADONAGA; Fiscal Year: 2004
    ..These studies should contribute to our fundamental understanding of gene expression and should be applicable to the analysis of human diseases that might be treated by the specific control of transcription factors or chromatin. ..
  29. Regulation of the Multiple Functions of Msi1p/Cac3p
    Stephen Johnston; Fiscal Year: 2003
    ..abstract_text> ..
  30. Function of the Origin Recognition Complex
    JOHN DANZER; Fiscal Year: 2006
    ....
  31. MOLECULAR BASIS OF CELL GROWTH AND TRANSFORMATION
    Robert Roeder; Fiscal Year: 1999
    ....
  32. X chromosome regulation in the C.elegans germline
    Christopher Bean; Fiscal Year: 2004
    ..abstract_text> ..
  33. FUNCTIONAL ANALYSIS OF CENTROMERIC CHROMATIN IN YEAST
    Molly Fitzgerald Hayes; Fiscal Year: 2003
    ..Scm3 is structurally related to Nap1, a chromatin assembly factor that associates with histones and facilitates formation of the nucleosome. A model is proposed for targeting of Cse4 by Scm3 that will be tested in aim 3. ..
  34. ESTROGEN RECEPTOR REGULATED TRANSCRIPTION
    David Shapiro; Fiscal Year: 2005
    ....
  35. Characterizing Sperm Chromatic Assembly in C. elegans
    DIANA CHU; Fiscal Year: 2005
    ..These factors may be conserved and play vital roles in fertility and reproduction in all organisms. ..
  36. TELOMERE ORGANIZATION AND FUNCTION IN S CERVISIAE
    Judith Berman; Fiscal Year: 2002
    ....
  37. Identification and analysis of substrate activators of the Aurora B kinase
    JILL SCHUMACHER; Fiscal Year: 2009
    ..Since Aurora substrate activators have the potential to impart spatial, temporal, and substrate specificity to the Aurora kinases, they are sure to be valuable targets for cancer therapy. ..
  38. Mechanism of Histone Recognition and Nucleosome Assembly
    Lynn Glowczewski; Fiscal Year: 2003
    ..These studies will improve understanding of the fundamental cellular mechanisms of chromatin assembly and silent chromatin formation. ..
  39. Signals Encoded in DNA that Affect Chromatin Structure
    Arnold Stein; Fiscal Year: 2003
    ..Information obtained here might ultimately enable one to predict functionally important aspects of chromatin structure computationally from DNA sequence data. ..
  40. Functional Analysis of Histone Deacetylases
    Edward Seto; Fiscal Year: 2006
    ..These studies will ultimately provide important insights critical for a thorough understanding of the intricate mechanisms operating to orchestrate gene expression in mammalian cells. ..
  41. X-ray area detector system for structural biology
    Mair Churchill; Fiscal Year: 2003
    ..M. Holers) Structural basis of ethanol's actions (D. Jones) Mechanism of DNA polymerase III replication (C. McHenry) Mechanisms of replication and cell-cycle control (R. Sclafani) Chromatin assembly and DNA repair (J. Tyler) ..
  42. Control of centromeric chromatin structure in Drosophila
    Takehito Furuyama; Fiscal Year: 2006
    ..Characterization of both protein factors and DNA elements that act in concert at the centromeres will lead to better understanding of centromere formation. [unreadable] [unreadable]..
  43. RSF: properties and dependence on histone modifications
    DAWN SCHMIDT; Fiscal Year: 2006
    ..Rsf-1 and its PHD domain will be probed for their ability to bind phosphoinositols, and if so, for their influence on RSF function. [unreadable] [unreadable]..
  44. Analysis of a Novel Cancer Pathway Containing PML & HIRA
    Peter Adams; Fiscal Year: 2005
    ..abstract_text> ..
  45. ALIGNMENT BASED METHODS FOR MOLECULAR SEQUENCE ANALYSIS
    Steven Henikoff; Fiscal Year: 2001
    ..This effort could yield new eukaryotic family members of great importance for understanding epigenetic phenomena, some of which, such as parental imprinting, are relevant to human disease. ..
  46. Epigenetic Control of Heterochromatin Formation
    Rabindranath De La Fuente; Fiscal Year: 2010
    ..abstract_text> ..
  47. Maintenance of blocks-based tools for functional genomic
    Steven Henikoff; Fiscal Year: 2006
    ..These various components will be packaged to facilitate updates and local implementations. [unreadable] [unreadable]..
  48. A genome-wide screen for regulators of senescence
    Peter Adams; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  49. Request for SCANCO micro-CT 40 Multi-User Bone Studies
    Jane Lian; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  50. FASEB Summer Conference on Transcriptional Regulation During Cell Growth
    Jessica Tyler; Fiscal Year: 2008
    ..As transcription is the ultimate endpoint for many regulatory processes an understanding of its mechanism will have profound implications for human health. [unreadable] [unreadable] [unreadable]..
  51. SYNTHESIS OF OSTEOCALCIN IN BONE
    Jane Lian; Fiscal Year: 2006
    ..These studies will establish the importance of bone selective transcription factor complexes for formation, maintenance and remodeling of bone tissue. ..
  52. Genetic Recombination/Genomic Rearrangements Conference
    LORRAINE SYMINGTON; Fiscal Year: 2005
    ..abstract_text> ..
  53. Genetic Recombination and Chromosome Rearrangements
    LORRAINE SYMINGTON; Fiscal Year: 2007
    ..Much of the conference will be devoted to topics of concern both to the National Cancer Institute and to the Institute of Environmental and Health Sciences. [unreadable] [unreadable] [unreadable] [unreadable]..
  54. MODULATION OF COLON CARCINOGENESIS BY CHLOROPHYLLIN
    Roderick Dashwood; Fiscal Year: 2008
    ..3C. Determine CHL effects on beta-catenin nuclear export and Adherins junction remodeling. 3D. Assess direct effects of CHL on a downstream target of beta-catenin, c-Jun. ..
  55. GENETICS OF HEART AND KIDNEY DAMAGE IN HYPERTENSION
    Christian Deschepper; Fiscal Year: 2004
    ..abstract_text> ..
  56. TARGETS OF METASTATIC PANCREATIC CANCER
    Christine Iacobuzio Donahue; Fiscal Year: 2008
    ..abstract_text> ..
  57. 2008 FASEB Summer Research Conference on Biological Methylation: from DNA to Hist
    Xiaodong Cheng; Fiscal Year: 2008
    ..In addition, three afternoon mini-symposia are devoted to young investigators. [unreadable] [unreadable] [unreadable] [unreadable]..
  58. The molecular role of lkaros in leukemogenesis
    Susan Winandy; Fiscal Year: 2009
    ..This system, in conjunction with the Ikaros null mice, makes us uniquely qualified to define the mechanistic role of this novel tumor suppressor gene. ..
  59. TRANSCRIPTIONAL CONTROL OF TH2 CELL DIFFERENTIATION
    Wei Ping Zheng; Fiscal Year: 2004
    ..It is proposed that knowledge gained from these studies will advance understanding and improve treatment of immunological diseases such as allergy and infection where Th2 responses are critical. ..
  60. Nanomedicine Center for Protein Deposition Diseases(RMI)
    Yuri Lyubchenko; Fiscal Year: 2004
    ..We assembled a multidisciplinary team of 12 investigators with broad expertise in nanobiology, imaging, spectroscopy, single molecule biophysics, proteomics, drug delivery and medicine. ..