prometaphase

Summary

Summary: The phase of cell nucleus division following PROPHASE, when the breakdown of the NUCLEAR ENVELOPE occurs and the MITOTIC SPINDLE APPARATUS enters the nuclear region and attaches to the KINETOCHORES.

Top Publications

  1. pmc The spatial arrangement of chromosomes during prometaphase facilitates spindle assembly
    Valentin Magidson
    Wadsworth Center, New York State Department of Health, Albany, NY 12201 509, USA
    Cell 146:555-67. 2011
  2. pmc Gamma-tubulin is required for bipolar spindle assembly and for proper kinetochore microtubule attachments during prometaphase I in Drosophila oocytes
    Stacie E Hughes
    Stowers Institute for Medical Research, Kansas City, Missouri, United States of America
    PLoS Genet 7:e1002209. 2011
  3. pmc Heterochromatic threads connect oscillating chromosomes during prometaphase I in Drosophila oocytes
    Stacie E Hughes
    Stowers Institute for Medical Research, Kansas City, Missouri, United States of America
    PLoS Genet 5:e1000348. 2009
  4. pmc Mitotic progression becomes irreversible in prometaphase and collapses when Wee1 and Cdc25 are inhibited
    Tamara A Potapova
    Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Mol Biol Cell 22:1191-206. 2011
  5. pmc The spindle checkpoint protein Mad2 regulates APC/C activity during prometaphase and metaphase of meiosis I in Saccharomyces cerevisiae
    Dai Tsuchiya
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    Mol Biol Cell 22:2848-61. 2011
  6. pmc Prometaphase APCcdh1 activity prevents non-disjunction in mammalian oocytes
    Alexandra Reis
    Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle NE2 4HH, UK
    Nat Cell Biol 9:1192-8. 2007
  7. pmc APC/C(Cdc20) targets E2F1 for degradation in prometaphase
    Melissa J Peart
    Department of Biological Sciences, Columbia University, New York, NY, USA
    Cell Cycle 9:3956-64. 2010
  8. pmc Hec1-dependent cyclin B2 stabilization regulates the G2-M transition and early prometaphase in mouse oocytes
    Liming Gui
    Mammalian Oocyte and Embryo Research Laboratory, Cell and Developmental Biology, UCL, London WC1E 6BT, UK
    Dev Cell 25:43-54. 2013
  9. pmc Increased CDK1 activity determines the timing of kinetochore-microtubule attachments in meiosis I
    Olga Davydenko
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
    J Cell Biol 202:221-9. 2013
  10. pmc The APC/C recruits cyclin B1-Cdk1-Cks in prometaphase before D box recognition to control mitotic exit
    Wouter van Zon
    Division of Molecular Biology and 2 Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, Amsterdam, Netherlands
    J Cell Biol 190:587-602. 2010

Detail Information

Publications209 found, 100 shown here

  1. pmc The spatial arrangement of chromosomes during prometaphase facilitates spindle assembly
    Valentin Magidson
    Wadsworth Center, New York State Department of Health, Albany, NY 12201 509, USA
    Cell 146:555-67. 2011
    ..data demonstrate that unstable lateral interactions between kinetochores and microtubules dominate during early prometaphase. These transient interactions lead to the reproducible arrangement of chromosomes in an equatorial ring on the ..
  2. pmc Gamma-tubulin is required for bipolar spindle assembly and for proper kinetochore microtubule attachments during prometaphase I in Drosophila oocytes
    Stacie E Hughes
    Stowers Institute for Medical Research, Kansas City, Missouri, United States of America
    PLoS Genet 7:e1002209. 2011
    ..b>Prometaphase I spindles in γtub37C mutant oocytes showed wide, non-tapered spindle poles and disrupted positioning...
  3. pmc Heterochromatic threads connect oscillating chromosomes during prometaphase I in Drosophila oocytes
    Stacie E Hughes
    Stowers Institute for Medical Research, Kansas City, Missouri, United States of America
    PLoS Genet 5:e1000348. 2009
    ..We observed that achiasmate homologs display dynamic movements on the meiotic spindle during mid-prometaphase. An analysis of living prometaphase oocytes revealed both the rejoining of achiasmate X chromosomes initially ..
  4. pmc Mitotic progression becomes irreversible in prometaphase and collapses when Wee1 and Cdc25 are inhibited
    Tamara A Potapova
    Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Mol Biol Cell 22:1191-206. 2011
    ..By inhibiting Cdk chemically, we showed that, in prometaphase, when Cdk1 substrates approach the peak of their phosphorylation, cells become capable of proper M-to-G1 ..
  5. pmc The spindle checkpoint protein Mad2 regulates APC/C activity during prometaphase and metaphase of meiosis I in Saccharomyces cerevisiae
    Dai Tsuchiya
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    Mol Biol Cell 22:2848-61. 2011
    ..most cells undergo both meiotic divisions, but securin, a substrate of the APC/C, is degraded prematurely, and prometaphase I/metaphase I is accelerated...
  6. pmc Prometaphase APCcdh1 activity prevents non-disjunction in mammalian oocytes
    Alexandra Reis
    Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle NE2 4HH, UK
    Nat Cell Biol 9:1192-8. 2007
    ..APC(cdh1) was active first, during prometaphase I, and was needed in order to allow homologue congression, as loss of cdh1 speeded up MI, leading to premature ..
  7. pmc APC/C(Cdc20) targets E2F1 for degradation in prometaphase
    Melissa J Peart
    Department of Biological Sciences, Columbia University, New York, NY, USA
    Cell Cycle 9:3956-64. 2010
    ..synchronization experiments showed that siRNA directed against Cdc20 induced an accumulation of E2F1 protein in prometaphase cells...
  8. pmc Hec1-dependent cyclin B2 stabilization regulates the G2-M transition and early prometaphase in mouse oocytes
    Liming Gui
    Mammalian Oocyte and Embryo Research Laboratory, Cell and Developmental Biology, UCL, London WC1E 6BT, UK
    Dev Cell 25:43-54. 2013
    ..These data identify another dimension to Hec1 function centered on M phase entry and early prometaphase progression and challenge the view that cyclin B2 is completely dispensable in mammals.
  9. pmc Increased CDK1 activity determines the timing of kinetochore-microtubule attachments in meiosis I
    Olga Davydenko
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
    J Cell Biol 202:221-9. 2013
    ..we show that during meiosis I, attachments are regulated by CDK1 activity, which gradually increases through prometaphase and metaphase I...
  10. pmc The APC/C recruits cyclin B1-Cdk1-Cks in prometaphase before D box recognition to control mitotic exit
    Wouter van Zon
    Division of Molecular Biology and 2 Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, Amsterdam, Netherlands
    J Cell Biol 190:587-602. 2010
    The ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C) is activated at prometaphase by mitotic phosphorylation and binding of its activator, Cdc20...
  11. ncbi Arecoline arrests cells at prometaphase by deregulating mitotic spindle assembly and spindle assembly checkpoint: implication for carcinogenesis
    Yu Chu Wang
    Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    Oral Oncol 46:255-62. 2010
    ..Here we reported that arecoline, a major alkaloid of areca nut, could arrest cells at prometaphase with large amounts of misaligned chromosomes...
  12. pmc APC/C(Cdc20) controls the ubiquitin-mediated degradation of p21 in prometaphase
    Virginia Amador
    Department of Pathology, NYU Cancer Institute, New York University School of Medicine, 550 First Avenue MSB 599, New York, NY 10016, USA
    Mol Cell 27:462-73. 2007
    ..G1/S transition, p21 proteolysis is mediated by Skp2; however, p21 reaccumulates in G2 and is degraded again in prometaphase. How p21 degradation is controlled in mitosis remains unexplored...
  13. pmc PICH and cotargeted Plk1 coordinately maintain prometaphase chromosome arm architecture
    Yasuhiro Kurasawa
    Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Biol Cell 21:1188-99. 2010
    ..we show that the Plk1-binding protein PICH and Plk1 kinase coordinately maintain chromosome architecture during prometaphase. PICH knockdown results in a loss of Plk1 from the chromosome arm and an increase in highly disorganized "wavy" ..
  14. ncbi Chk1 is required for spindle checkpoint function
    George Zachos
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Glasgow G61 1BD, United Kingdom
    Dev Cell 12:247-60. 2007
    ..In addition, Chk1-deficient cells exhibit increased resistance to taxol. These results suggest a mechanism through which Chk1 could protect against tumorigenesis through its role in spindle checkpoint signaling...
  15. ncbi c-MYC delays prometaphase by direct transactivation of MAD2 and BubR1: identification of mechanisms underlying c-MYC-induced DNA damage and chromosomal instability
    Antje Menssen
    Molecular Oncology, Max Planck Institute of Biochemistry, Martinsried, Germany
    Cell Cycle 6:339-52. 2007
    ....
  16. pmc The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly
    Arturo V Orjalo
    Sections of Cell and Developmental Biology, Division of Biological Sciences, University of California San Diego Medical School, La Jolla, CA 92093 0347, USA
    Mol Biol Cell 17:3806-18. 2006
    ..Each antibody stained not only kinetochores but also prometaphase spindle poles and proximal spindle fibers, mirroring the dual prometaphase localization of the spindle ..
  17. pmc Hypoxia transiently sequesters mps1 and polo to collagenase-sensitive filaments in Drosophila prometaphase oocytes
    William D Gilliland
    Stowers Institute for Medical Research, Kansas City, Missouri, United States of America
    PLoS ONE 4:e7544. 2009
    ..for proper cell cycle regulation in meiosis and mitosis, localize to numerous ooplasmic filaments during prometaphase in Drosophila oocytes...
  18. pmc Role of cyclin B1/Cdc2 up-regulation in the development of mitotic prometaphase arrest in human breast cancer cells treated with nocodazole
    Hye Joung Choi
    Department of Pharmacology, Toxicology and Therapeutics, School of Medicine, University of Kansas Medical Center, Kansas City, Kansas, United States of America
    PLoS ONE 6:e24312. 2011
    ..cells with nocodazole, a prototypic microtubule inhibitor, results in strong up-regulation of cyclin B1 and Cdc2 levels, and their increases are required for the development of mitotic prometaphase arrest and characteristic phenotypes.
  19. pmc Critical role of cyclin B1/Cdc2 up-regulation in the induction of mitotic prometaphase arrest in human breast cancer cells treated with 2-methoxyestradiol
    Hye Joung Choi
    Department of Pharmacology, University of Kansas Medical Center, Kansas City, KS, USA
    Biochim Biophys Acta 1823:1306-15. 2012
    ..We found that 2ME(2) can selectively induce mitotic prometaphase arrest, but not G(2) phase arrest, in cultured MDA-MB-435s and MCF-7 human breast cancer cells...
  20. pmc The Caenorhabditis elegans kinetochore reorganizes at prometaphase and in response to checkpoint stimuli
    Jeffrey H Stear
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Mol Biol Cell 15:5187-96. 2004
    ..Here, we show that the Caenorhabditis elegans kinetochore displays analogous rearrangements at prometaphase, when microtubule/chromosome interactions are being established, and after exposure to checkpoint stimuli such ..
  21. pmc Chromosome congression in the absence of kinetochore fibres
    Shang Cai
    Indiana University, Bloomington, 47405, USA
    Nat Cell Biol 11:832-8. 2009
    ..These bi-oriented connections are also used to maintain stable chromosome alignment at the spindle equator...
  22. pmc A spindle assembly checkpoint protein functions in prophase I arrest and prometaphase progression
    Hayden Homer
    Oocyte and Embryo Research Laboratory, Department of Cell and Developmental Biology, Division of Biosciences and Institute for Women s Health, University College London, London, UK
    Science 326:991-4. 2009
    ..Both meiotic defects in BubR1-depleted oocytes are due to reduced activity of the master regulator known as the anaphase-promoting complex (APC), brought about through diminished levels of the APC coactivator Cdh1...
  23. pmc The mechanism of secondary nondisjunction in Drosophila melanogaster females
    Youbin Xiang
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Genetics 174:67-78. 2006
    ....
  24. ncbi Aurora A regulates prometaphase progression by inhibiting the ability of RASSF1A to suppress APC-Cdc20 activity
    Su Jung Song
    National Research Laboratory for Genomic Stability, Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Korea
    Cancer Res 69:2314-23. 2009
    ..RNA interference and expression of a nonphosphorylatable RASSF1A (S203A) mutant gene led to a marked delay in prometaphase progression...
  25. pmc Different phosphorylation states of the anaphase promoting complex in response to antimitotic drugs: a quantitative proteomic analysis
    Judith A J Steen
    Departments of Neurobiology and Pathology, Harvard Medical School and Children s Hospital Boston, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:6069-74. 2008
    ..We examined the phosphorylation patterns of the APC in HeLa S3 cells after they were arrested in prometaphase with taxol, nocodazole, vincristine, or monastrol...
  26. ncbi Efficient chromosome capture requires a bias in the 'search-and-capture' process during mitotic-spindle assembly
    R Wollman
    Laboratory of Cell and Computational Biology, Center for Genetics and Development, University of California, Davis, Davis, California 95616, USA
    Curr Biol 15:828-32. 2005
    The mitotic spindle assembles into a bipolar, microtubule-based protein machine during prometaphase. One proposed mechanism for this process is "search-and-capture," in which dynamically unstable microtubules (MTs) search space ..
  27. pmc An integrated mechanobiochemical feedback mechanism describes chromosome motility from prometaphase to anaphase in mitosis
    Jian Liu
    Center for Theoretical Biological Physics, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 105:13752-7. 2008
    ..This model can recapitulate all of the essential and distinct features of chromosome motilities from prometaphase to anaphase in a coherent manner...
  28. pmc Securin and separase phosphorylation act redundantly to maintain sister chromatid cohesion in mammalian cells
    Xingxu Huang
    Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Biol Cell 16:4725-32. 2005
    ..Securin(-/-)separase(+/S1121A) cells are viable but fail to maintain sister chromatid cohesion in response to the disruption of spindle microtubules, show enhanced sensitivity to nocodazole, and cannot recover from prometaphase arrest.
  29. ncbi Chromosome damage in mitosis induces BubR1 activation and prometaphase arrest
    Eunhee Choi
    Department of Biological Sciences and Research Center for Functional Cellulomics, Seoul National University, San 56 1, Shinlim Dong, Gwanak ku, Seoul 151 742, Republic of Korea
    FEBS Lett 582:1700-6. 2008
    ..and Western blot analysis in synchronized cells, we provide evidence that DSBs activate BubR1, leading to prometaphase arrest...
  30. pmc Three-dimensional maps of all chromosomes in human male fibroblast nuclei and prometaphase rosettes
    Andreas Bolzer
    Department of Biology II, Anthropology and Human Genetics, Ludwig Maximilians University, Munich, Germany
    PLoS Biol 3:e157. 2005
    ..We present unequivocal evidence for a probabilistic 3D order of prometaphase chromosomes, as well as of chromosome territories (CTs) in nuclei of quiescent (G0) and cycling (early S-phase) ..
  31. pmc Prolonged prometaphase blocks daughter cell proliferation despite normal completion of mitosis
    Yumi Uetake
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, 01605, USA
    Curr Biol 20:1666-71. 2010
    ..that once the checkpoint is satisfied and cells divide, the daughter cells would proliferate regardless of prometaphase duration...
  32. pmc Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope
    Gul Civelekoglu-Scholey
    Department of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA
    J Cell Biol 188:49-68. 2010
    We tested the classical hypothesis that astral, prometaphase bipolar mitotic spindles are maintained by balanced outward and inward forces exerted on spindle poles by kinesin-5 and -14 using modeling of in vitro and in vivo data from ..
  33. ncbi Securin associates with APCCdh1 in prometaphase but its destruction is delayed by Rae1 and Nup98 until the metaphase/anaphase transition
    Karthik B Jeganathan
    Department of Pediatric and Adolescent Medicine, Mayo College of Medicine, Rochester, Minnesota 55905, USA
    Cell Cycle 5:366-70. 2006
    ..We found that Rae1 and Nup98 assemble into a complex with APC(Cdh1) in prometaphase and act to delay APC(Cdh1)-mediated ubiquitination of securin until the metaphase/anaphase transition...
  34. ncbi [Dynamics of microtubular cytoskeleton in higher plant meiosis. V. Late prometaphase. The general scheme of anastral spindle formation]
    N V Shamina
    Tsitologiia 47:889-97. 2005
    ..of cereal wide hybrids, haploids and meiotic mutants, the processes involved in cytoskeleton cycle at late prometaphase (a sub-stage of transition from chaotic figure to bipolar spindle) were studied...
  35. ncbi BubR1 and CENP-E have antagonistic effects upon the stability of microtubule-kinetochore attachments in Drosophila S2 cell mitosis
    André F Maia
    IBMC, Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal
    Cell Cycle 6:1367-78. 2007
    ..cells exit mitosis prematurely due to loss of SAC activity, CENP-meta-depleted cells accumulate in prometaphase and do not exit mitosis after spindle damage...
  36. ncbi [Dynamics of microtubular cytoskeleton in higher plant meiosis. IV. Middle prometaphase]
    N V Shamina
    Tsitologiia 47:760-5. 2005
    ..abnormalities in pollen mother cells of cereal distant hybrids, processes of cytoskeleton cycle at the middle prometaphase (chaotic stage) were studied...
  37. ncbi Human Scc4 is required for cohesin binding to chromatin, sister-chromatid cohesion, and mitotic progression
    Erwan Watrin
    Research Institute of Molecular Pathology I M P, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Curr Biol 16:863-74. 2006
    ..Mutation of Scc2 orthologs causes defects in cohesion, transcription, and development, resulting in Cornelia de Lange syndrome in humans...
  38. ncbi Kinetochore-microtubule interactions "in check" by Bub1, Bub3 and BubR1: The dual task of attaching and signalling
    Elsa Logarinho
    Life and Health Sciences Research Institute ICVS, School of Health Sciences, University of Minho, Braga, Portugal
    Cell Cycle 7:1763-8. 2008
    ....
  39. pmc The V260I mutation in fission yeast alpha-tubulin Atb2 affects microtubule dynamics and EB1-Mal3 localization and activates the Bub1 branch of the spindle checkpoint
    Kazuhide Asakawa
    Laboratory of Cell Regulation, Cancer Research UK, London Research Institute, Lincoln s Inn Fields Laboratories, London WC2A 3PX, UK
    Mol Biol Cell 17:1421-35. 2006
    ..Furthermore in atb2-983 back-and-forth centromere oscillation during prometaphase is abolished...
  40. ncbi Doublecortin-like kinase controls neurogenesis by regulating mitotic spindles and M phase progression
    Tianzhi Shu
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Neuron 49:25-39. 2006
    ..mechanism, DCLK regulates the formation of bipolar mitotic spindles and the proper transition from prometaphase to metaphase during mitosis...
  41. ncbi Atomic force microscope tracking observation of Chinese hamster ovary cell mitosis
    Yangzhe Wu
    Chemistry Department, Jinan University, Guangzhou 510632, China
    Micron 37:139-45. 2006
    ..of Chinese hamster ovary (CHO) cell chromosomes during various mitotic phases, including typical prophase, prometaphase, metaphase, anaphase and telophase...
  42. ncbi Caspase-independent apoptosis is activated by diazepam-induced mitotic failure in HeLa cells, but not in human primary fibroblasts
    I Vitale
    Department of Biology, University Roma Tre, V le Marconi 446, Rome, 00146, Italy
    Apoptosis 10:909-20. 2005
    ..In fact, differently from fibroblasts, which undergo a transient block in prophase-to-prometaphase transition, a high proportion of tumor cells attempt to escape from the DZ-imposed mitotic block, fail to ..
  43. ncbi Chromosome ideograms of the laboratory rat (Rattus norvegicus) based on high-resolution banding, and anchoring of the cytogenetic map to the DNA sequence by FISH in sample chromosomes
    A Hamta
    CMB Genetics, Goteborg University, Goteborg, Sweden
    Cytogenet Genome Res 115:158-68. 2006
    A detailed banded ideogram representation of the rat chromosomes was constructed based on actual G-banded prometaphase chromosomes...
  44. pmc CENP-A is required for accurate chromosome segregation and sustained kinetochore association of BubR1
    Vinciane Regnier
    Department of Biochemistry, Oxford University, South Parks Road, OX1 3QU Oxford, United Kingdom
    Mol Cell Biol 25:3967-81. 2005
    ..chromosomes are deficient in proper congression on the mitotic spindle and there is a transient delay in prometaphase. CENP-A-depleted cells further proceed through anaphase and cytokinesis with unequal chromosome segregation, ..
  45. ncbi Caffeine yields aneuploidy through asymmetrical cell division caused by misalignment of chromosomes
    Yoko Katsuki
    Department of Pediatrics and Developmental Biology, Graduate Medical School, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    Cancer Sci 99:1539-45. 2008
    ..caffeine-treated mitotic cells showed misalignment of chromosomes at the metaphase plates, and were arrested at prometaphase. Mitoticarrest deficient 2 (MAD2) depletion rescued the caffeine-induced delay of mitotic exit, indicating that ..
  46. pmc Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis
    Fabian Oceguera-Yanez
    Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606 8501, Japan
    J Cell Biol 168:221-32. 2005
    ..Depletion of Ect2 also impairs microtubule attachment to kinetochores and causes prometaphase delay and abnormal chromosomal segregation, as does depletion of Cdc42 or expression of the Ect2 and MgcRacGAP ..
  47. ncbi Distinct functions of condensin I and II in mitotic chromosome assembly
    Toru Hirota
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    J Cell Sci 117:6435-45. 2004
    ..of cohesin from chromosome arms, for chromosome shortening and for normal timing of progression through prometaphase and metaphase, whereas normal condensin II levels are dispensable for these processes...
  48. pmc Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase
    Rita Gandhi
    Cold Spring Harbor Laboratory, One Bungtown Road, P O Box 100, Cold Spring Harbor, New York 11724, USA
    Curr Biol 16:2406-17. 2006
    ..This process is known as sister-chromatid resolution. Although two mitotic kinases have been implicated in this process, it remains unknown exactly how the cohesin-mediated linkage is destabilized at a mechanistic level...
  49. ncbi Activation of a novel ataxia-telangiectasia mutated and Rad3 related/checkpoint kinase 1-dependent prometaphase checkpoint in cancer cells by diallyl trisulfide, a promising cancer chemopreventive constituent of processed garlic
    Anna Herman-Antosiewicz
    Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Mol Cancer Ther 6:1249-61. 2007
    ..and Rad3 related (ATR)/checkpoint kinase 1 (Chk1)-dependent checkpoint partially responsible for DATS-mediated prometaphase arrest in cancer cells, which is different from the recently described gamma irradiation-induced mitotic exit ..
  50. ncbi Pharmacologic inhibition of CDC25 phosphatases impairs interphase microtubule dynamics and mitotic spindle assembly
    Martine Cazales
    LBCMCP CNRS UMR5088 IFR109, Universite Paul Sabatier, 118 route de Narbonne, 31062 Toulouse, France
    Mol Cancer Ther 6:318-25. 2007
    ..Our results show a role for CDC25 phosphatases in regulating microtubule dynamics throughout the cell cycle and suggest that combinations of CDC25 inhibitors with microtubule-targeting agents may be of therapeutic value...
  51. ncbi Matrimony ties Polo down: can this kinase get free?
    S Kendall Smith
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Cell Cycle 7:698-701. 2008
    ..The termination of G(2) arrest (as defined by NEB) and the subsequent entry of the oocyte into prometaphase is regulated by an intracellular signaling cascade whose ultimate feature is the activation of the cyclin B-..
  52. ncbi Farnesyl transferase inhibitors impair chromosomal maintenance in cell lines and human tumors by compromising CENP-E and CENP-F function
    Katherine Schafer-Hales
    Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA
    Mol Cancer Ther 6:1317-28. 2007
    ..The data show that lonafarnib in proliferating cancer cells depletes CENP-E and CENP-F from metaphase but not prometaphase kinetochores...
  53. pmc Moesin and its activating kinase Slik are required for cortical stability and microtubule organization in mitotic cells
    Sebastien Carreno
    Centre de Biologie du Developpement, Université Toulouse III Centre National de la Recherche Scientifique, Unite Mixte de Recherche 5547, 31062 Toulouse Cedex 09, France
    J Cell Biol 180:739-46. 2008
    ..Activated moesin homogenously localizes at the cortex in prometaphase and is progressively restricted at the equator in later stages...
  54. ncbi Okadaic acid: chromosomal non-disjunction analysis in human lymphocytes and study of aneugenic pathway in CHO-K1 cells
    Ludovic Le Hegarat
    AFSSA, Laboratoire d Etudes et de Recherches sur les Médicaments Vétérinaires et les Désinfectants, Unité de Toxicologie Génétique des Contaminants Alimentaires, B P 90203, 35302 Fougeres, France
    Mutat Res 578:53-63. 2005
    ..The results showed that OA induced a progressive accumulation of mitotic CHO-K1 cells in prometaphase, an induction of multipolar mitotic spindle with centrosome amplification and the formation of multinucleated ..
  55. pmc The human Nup107-160 nuclear pore subcomplex contributes to proper kinetochore functions
    Michela Zuccolo
    Centre de Recherche, UMR 144 CNRS, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    EMBO J 26:1853-64. 2007
    ..Together, our data thus provide the first molecular clues underlying the function of the human Nup107-160 complex at kinetochores...
  56. pmc Prophase microtubule arrays undergo flux-like behavior in mammalian cells
    Nick P Ferenz
    Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01003, USA
    Mol Biol Cell 18:3993-4002. 2007
    ..We propose that the marked reduction in rate and directionality of microtubule motion from prophase to metaphase results from changes in microtubule organization during spindle formation...
  57. ncbi Self-regulated Plk1 recruitment to kinetochores by the Plk1-PBIP1 interaction is critical for proper chromosome segregation
    Young H Kang
    Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    Mol Cell 24:409-22. 2006
    ..Thus, Plk1 self-regulates the Plk1-PBIP1 interaction to timely localize to the kinetochores and promote proper chromosome segregation...
  58. pmc Two components of the Myb complex, DMyb and Mip130, are specifically associated with euchromatin and degraded during prometaphase throughout development
    George S Scaria
    Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, 900 South Ashland Avenue, 2370 MBRB, Chicago IL 60607 7170, USA
    Mech Dev 125:646-61. 2008
    ..Furthermore, DMyb and Mip130 are unstable proteins that are degraded during prometaphase of mitosis...
  59. ncbi DNA changes involved in the formation of metaphase chromosomes, as observed in mouse duodenal crypt cells stained by osmium-ammine. I. New structures arise during the S phase and condense at prophase into "chromomeres," which fuse at prometaphase into mit
    M El-Alfy
    Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada
    Anat Rec 242:433-48. 1995
    ....
  60. ncbi When rDNA transcription is arrested during mitosis, UBF is still associated with non-condensed rDNA
    J Gebrane-Younes
    Institut Jacques Monod, Paris, France
    J Cell Sci 110:2429-40. 1997
    ..In PtK1 cells, rDNA transcription was still active in prophase, stopped in prometaphase until early anaphase, and activated in late anaphase...
  61. ncbi The aurora B kinase AIR-2 regulates kinetochores during mitosis and is required for separation of homologous Chromosomes during meiosis
    Susanne Kaitna
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030, Vienna, Austria
    Curr Biol 12:798-812. 2002
    ..The mechanisms responsible for orienting kinetochores in mitosis and for causing asynchronous loss of cohesion during meiosis are not well understood...
  62. ncbi [Dynamic changes of gamma-tubulin in preimplantation development of parthenogenetic mouse embryos.]
    Qing Hua Zhang
    Department of Histology and Embryology, Harbin Medical University, Harbin, China
    Sheng Li Xue Bao 60:777-82. 2008
    ..At 24 h after culture, i.e. at prometaphase, gamma-tubulin migrated along the spindle microtubule to the two poles...
  63. ncbi The first mitosis of the mouse embryo is prolonged by transitional metaphase arrest
    Marta Sikora-Polaczek
    Department of Embryology, Institute of Zoology, Warsaw University, 02 096 Warsaw, Poland
    Biol Reprod 74:734-43. 2006
    ..The mechanism of the prolongation of the first mitosis remains unknown, and it is not clear whether prometaphase or metaphase or both are prolonged...
  64. pmc A perikinetochoric ring defined by MCAK and Aurora-B as a novel centromere domain
    María Teresa Parra
    Departamento de Biologia, Universidad Autonoma de Madrid, Madrid, Spain
    PLoS Genet 2:e84. 2006
    ..also colocalise at the inner centromere domain as a band that joins sister kinetochores, but only during prometaphase II in unattached chromosomes...
  65. pmc Overexpression of the dynamitin (p50) subunit of the dynactin complex disrupts dynein-dependent maintenance of membrane organelle distribution
    J K Burkhardt
    The University of Chicago, Department of Pathology, Chicago, Illinois 60637, USA
    J Cell Biol 139:469-84. 1997
    ..of one dynactin subunit, dynamitin, disrupts the complex, resulting in dissociation of cytoplasmic dynein from prometaphase kinetochores, with consequent perturbation of mitosis (Echeverri, C.J., B.M. Paschal, K.T. Vaughan, and R.B...
  66. pmc Bipolar spindle attachments affect redistributions of ZW10, a Drosophila centromere/kinetochore component required for accurate chromosome segregation
    B C Williams
    Section of Genetics and Development, Cornell University, Ithaca, New York 14853 2703, USA
    J Cell Biol 134:1127-40. 1996
    ..ZW10 is first visible during prometaphase, where it localizes to the kinetochores of the bivalent chromosomes (during meiosis I) or to the sister ..
  67. ncbi Microtubule disassembly delays the G2-M transition in vertebrates
    C L Rieder
    Laboratory of Cell Regulation, Division of Molecular Medicine, The Wadsworth Center, New York State Department of Health, Albany, 12201 0509, USA
    Curr Biol 10:1067-70. 2000
    ..from entering prophase and, as the MI does not increase during this time, existing prophase cells do not enter prometaphase. When mid-prophase cells were treated with nocodazole, the majority (70%) decondensed their chromosomes and ..
  68. ncbi PBC68: a nuclear pore complex protein that associates reversibly with the mitotic spindle
    P A Theodoropoulos
    Department of Basic Sciences and Department of Internal Medicine, The University of Crete, School of Medicine, Crete, Greece
    J Cell Sci 112:3049-59. 1999
    ..The autoantigen is modified at early prophase and disassembles at prometaphase concurrently with the breakdown of the nuclear envelope...
  69. ncbi Mitotic phosphoepitopes are expressed in Kc cells, neuroblasts and isolated chromosomes of Drosophila melanogaster
    H Bousbaa
    Centro de Citologia Experimental da Universidade do Porto, Portugal
    J Cell Sci 110:1979-88. 1997
    ..The 3F3/2 staining is retained in Kc cells and third instar larval neuroblasts arrested at the prometaphase state with microtubule inhibitors...
  70. ncbi The role of pre- and post-anaphase microtubules in the cytokinesis phase of the cell cycle
    J C Canman
    Department of Biology, University of North Carolina Chapel Hill, North Carolina 27599, USA
    Curr Biol 10:611-4. 2000
    ..that microinjection of antibodies to the spindle checkpoint protein Mad2 (mitotic arrest deficient) in prometaphase abrogates the spindle checkpoint, producing premature chromosome separation, segregation, and normal ..
  71. ncbi Cytochalasin D and latrunculin affect chromosome behaviour during meiosis in crane-fly spermatocytes
    A Forer
    Biology Department, York University, Downsview, Ontario, Canada
    Chromosome Res 6:533-49. 1998
    ..When applied in mid-prometaphase to metaphase, both drugs altered subsequent anaphase poleward movements: half-bivalents either moved more ..
  72. ncbi Fertilization differently affects the levels of cyclin B1 and M-phase promoting factor activity in maturing and metaphase II mouse oocytes
    Anna Ajduk
    Department of Embryology, Institute of Zoology, University of Warsaw, Warsaw, Poland
    Reproduction 136:741-52. 2008
    ..In this paper, we show that neither mono- nor polyspermic fertilization of prometaphase I and metaphase I oocytes triggered degradation of cyclin B1...
  73. ncbi F-actin distribution and function during sexual differentiation in Schizosaccharomyces pombe
    J Petersen
    Department of Genetics, Institute of Molecular Biology, University of Copenhagen, Copenhagen, Denmark
    J Cell Sci 111:867-76. 1998
    ..dots were randomly scattered during the horsetail movement that precedes meiosis I and remained scattered until prometaphase or metaphase of meiosis II, when they concentrated around the nucleus...
  74. ncbi Degradation of securin in mouse and pig oocytes is dependent on ubiquitin-proteasome pathway and is required for proteolysis of the cohesion subunit, Rec8, at the metaphase-to-anaphase transition
    Li Jun Huo
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Front Biosci 11:2193-202. 2006
    ..after germinal vesicle breakdown, immunoreactive securin accumulated around the condensed chromosomes at prometaphase I...
  75. ncbi Promoter methylation of CHFR gene in gastric carcinoma tissues detected using two methods
    Zhao Dong Cheng
    Department of Gerontology, Province Hospital of Anhui Medical University, Hefei, Anhui 230001, PR China
    Chin J Cancer 29:163-6. 2010
    ..Checkpoint with fork head-associated and ring finger (CHFR) governs the transition from prophase to prometaphase in response to mitotic stress...
  76. ncbi Localization of mitotic arrest deficient 1 (MAD1) in mouse oocytes during the first meiosis and its functions as a spindle checkpoint protein
    Dong Zhang
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Biol Reprod 72:58-68. 2005
    ..It was found that MAD1 was present in the oocytes from the GV to prometaphase I stages around the nuclei...
  77. ncbi Kinesin-like molecules involved in spindle formation
    V I Rodionov
    A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Russia
    J Cell Sci 106:1179-88. 1993
    ..head domain of Drosophila kinesin heavy chain (HD antibody) was microinjected into PtK1 cells at the prophase-prometaphase transition. Progress of the cell through mitosis was recorded for subsequent detailed analysis...
  78. pmc Kinetochore fiber maturation in PtK1 cells and its implications for the mechanisms of chromosome congression and anaphase onset
    B F McEwen
    Wadsworth Center, Division of Molecular Medicine, New York State Department of Health, Albany, New York 12201 0509, USA
    J Cell Biol 137:1567-80. 1997
    ..At late prometaphase, the average number of Mts on fully congressed kinetochores is 19.7 +/- 6.7 (n = 94), at late metaphase 24...
  79. ncbi Centrosome and microtubule dynamics during early stages of meiosis in mouse oocytes
    A Can
    Laboratory for Reproductive Cell Science, Department of Histology Embryology, Ankara University School of Medicine, Ankara, Sihhiye, 06339, Turkey
    Mol Hum Reprod 9:749-56. 2003
    ..concordance with MT polymerization in earlier stages of oocyte maturation from germinal vesicle stage (GV) to prometaphase I still remains unclear...
  80. ncbi Cyclin A and Nek2A: APC/C-Cdc20 substrates invisible to the mitotic spindle checkpoint
    Wouter van Zon
    Division of Molecular Carcinogenesis B7, The Netherlands Cancer Institute NKI AvL, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Biochem Soc Trans 38:72-7. 2010
    ..of a functional spindle checkpoint degrade cyclin B1 and securin right after nuclear-envelope breakdown, in prometaphase. Interestingly, two APC/C substrates, cyclin A and Nek2A, are normally degraded at nuclear-envelope breakdown, ..
  81. ncbi Regulation of intracellular MEK1/2 translocation in mouse oocytes: cytoplasmic dynein/dynactin-mediated poleward transport and cyclin B degradation-dependent release from spindle poles
    Bo Xiong
    State Key Laboratory of Reproductive Biology, Institute of Zoology and Graduate School, Chinese Academy of Sciences, Beijing, PR China
    Cell Cycle 6:1521-7. 2007
    ..In the present study, we investigated the mechanisms of poleward MEK1/2 transport during the prometaphase I/metaphase I transition and MEK1/2 release from the spindle poles during the metaphase I/anaphase I transition ..
  82. ncbi The cytoskeleton-dependent localization of cdc2/cyclin B in blastomere cortex during Xenopus embryonic cell cycle
    Norihiko Nakamura
    Department of Biological Science, Faculty of Science, Yamaguchi University, Yamaguchi, Japan
    Mol Reprod Dev 72:336-45. 2005
    ..in the cortical cytoplasm throughout the cell cycle, in the centrosomes and the nucleus at interphase and prometaphase, and in the spindles at metaphase and anaphase...
  83. pmc Kinetic suppression of microtubule dynamic instability by griseofulvin: implications for its possible use in the treatment of cancer
    Dulal Panda
    School of Biosciences and Bioengineering, Indian Institute of Technology, Bombay 400076, India
    Proc Natl Acad Sci U S A 102:9878-83. 2005
    ..Griseofulvin inhibited cell-cycle progression at prometaphase/anaphase of mitosis in parallel with its ability to inhibit cell proliferation...
  84. ncbi Distribution of 45S and 5S rDNA sites in 23 species of Eleocharis (Cyperaceae)
    Carlos Roberto Maximiano da Silva
    Pós graduação em Genética, Instituto de Biociencias, Letras e Ciencias Exatas, UNESP, Sao Jose do Rio Preto, SP 15054 000, Brazil
    Genetica 138:951-7. 2010
    ..occurrence of multiple 45S rDNA sites at terminal positions, and in the decondensed state of these regions in prometaphase/metaphase...
  85. ncbi Importin beta is transported to spindle poles during mitosis and regulates Ran-dependent spindle assembly factors in mammalian cells
    Marilena Ciciarello
    Institute of Molecular Biology and Pathology, Section of Genetics, CNR Consiglio Nazionale delle Ricerche, Via degli Apuli 4, Rome 00185, Italy
    J Cell Sci 117:6511-22. 2004
    ..This localization is temporally regulated from prometaphase until anaphase, when importin beta dissociates from poles and is recruited back around reforming nuclei...
  86. ncbi Distribution of eu- and heterochromatin in Plantago ovata
    M K Dhar
    School of Biotechnology, University of Jammu, Jammu, India
    Cytogenet Genome Res 125:235-40. 2009
    ..7%. Applying Giemsa C-banding and fluorescence in situ hybridization (FISH) using the Cot-1 fraction of DNA on prometaphase and metaphase chromosomes we investigated the chromosomal distribution of eu- and heterochromatin...
  87. ncbi Mitotic microtubule development and histone H3 phosphorylation in the holocentric chromosomes of Rhynchospora tenuis (Cyperaceae)
    M Guerra
    Departamento de Botanica, Centro de Ciencias Biologicas, Cidade Universitaria, 50 670 420, Recife, PE, Brazil
    Genetica 126:33-41. 2006
    ..tenuis coincide with those of other species namely: the absence of primary constriction in prometaphase and metaphase, and the parallel separation of sister chromatids at anaphase...
  88. ncbi Clathrin in mitotic spindles
    C T Okamoto
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles 90089 9121, USA
    Am J Physiol Cell Physiol 279:C369-74. 2000
    ..Staining of the mitotic spindle was evident in mitotic cells from prometaphase to telophase and in spindles in mitotic cells released from a thymidine-nocodazole block...
  89. pmc Active MAP kinase in mitosis: localization at kinetochores and association with the motor protein CENP-E
    M Zecevic
    Department of Microbiology and Cancer Center, University of Virginia, Health Sciences Center, Charlottesville, Virginia 22908, USA
    J Cell Biol 142:1547-58. 1998
    ..was constant from the time of nuclear envelope breakdown, the kinetochore labeling first appeared at early prometaphase, started to fade during chromosome congression, and then disappeared at midanaphase...
  90. pmc Functional dynamics of Polo-like kinase 1 at the centrosome
    Kazuhiro Kishi
    David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Cell Biol 29:3134-50. 2009
    ..This resulted in a G(2) delay, followed by a prominent prometaphase arrest, as a consequence of defective spindle formation and activation of the spindle checkpoint...
  91. pmc CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis
    H Liao
    Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, Pennsylvania 19111, USA
    J Cell Biol 130:507-18. 1995
    ..During mitosis, CENP-F is associated with kinetochores from prometaphase until early anaphase and is then detected at the spindle midzone throughout the remainder of anaphase...
  92. ncbi Proteolytic activity of the 26S proteasome is required for the meiotic resumption, germinal vesicle breakdown, and cumulus expansion of porcine cumulus-oocyte complexes matured in vitro
    Young Joo Yi
    Division of Animal Sciences, University of Missouri Columbia, Columbia, MO 65211, USA
    Biol Reprod 78:115-26. 2008
    ..Treatment with 10 microM MG132 arrested 28.4% of oocytes in GV stage (vs. 1.3% in control), 43.1% in prometaphase I, and 16.2% in metaphase I, whereas 83...
  93. ncbi Precocious (pre-anaphase) cleavage furrows in Mesostoma spermatocytes
    Arthur Forer
    Biology Department, York University, 4700 Keele Street, North York, Ontario, Canada M3J 1P3
    Eur J Cell Biol 89:607-18. 2010
    ..Cleavage furrows are initiated during prometaphase in spermatocytes of the flatworm Mesostoma, becoming detectable soon after the spindles achieve bipolarity...
  94. ncbi The mechanism of kinetochore-spindle attachment and polewards movement analyzed in PtK2 cells at the prophase-prometaphase transition
    A Merdes
    European Molecular Biology Laboratory, Heidelberg Federal Republic of Germany
    Eur J Cell Biol 53:313-25. 1990
    PtK2 cells at the prophase-prometaphase transition were analyzed to study the origin of kinetochore microtubules, the mode of kinetochore fiber construction and the mechanism of polewards movement...
  95. ncbi Ubiquitination and proteasomal degradation of the BRCA1 tumor suppressor is regulated during cell cycle progression
    Atish D Choudhury
    Institute for Cancer Genetics, Department of Pathology, Columbia University, New York, New York 10032, USA
    J Biol Chem 279:33909-18. 2004
    ..Formation of these conjugates occurs throughout G1 and S, but not in cells arrested in prometaphase by nocodazole...
  96. ncbi Genotype-phenotype correlations and clinical diagnostic criteria in Wolf-Hirschhorn syndrome
    M Zollino
    Istituto di Genetica Medica, Facolta di Medicina A Gemelli, UCSC, Rome, Italy
    Am J Med Genet 94:254-61. 2000
    ..Hemizygosity of 4p16.3 was detected by conventional prometaphase chromosome analysis (11 patients) or by molecular probes on apparently normal chromosomes (4 patients)...
  97. ncbi [Physical mapping of the 45S rDNA and 5S rDNA to rice prometaphase chromosome]
    Zhi yun Gong
    Agriculture College, Yangzhou University, Yangzhou 225009, China
    Yi Chuan Xue Bao 29:241-4. 2002
    ..the chromosomes related to 45S rDNA and 5S rDNA, the digoxigenin-dUTP labeled probe DNA was probed to the prometaphase chromosomes which were prepared from the root tips harvested from an indica rice variety, Zhongxian 3037...
  98. ncbi Localization of CENP-E in the fibrous corona and outer plate of mammalian kinetochores from prometaphase through anaphase
    C A Cooke
    Institute of Cell and Molecular Biology, University of Edinburgh, Michael Swann Building, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
    Chromosoma 106:446-55. 1997
    ..8 nm colloidal gold particles, CENP-E was localized primarily to the fibrous corona of the kinetochore in prometaphase and metaphase cells. Some labeling of the kinetochore outer plate was also observed...
  99. pmc Degradation of cyclin A is regulated by acetylation
    F Mateo
    Faculty of Medicine, Department of Cell Biology and Pathology, University of Barcelona, Barcelona, Spain
    Oncogene 28:2654-66. 2009
    Cyclin A accumulates at the onset of S phase, remains high during G(2) and early mitosis and is degraded at prometaphase. Here, we report that the acetyltransferase P/CAF directly interacts with cyclin A that as a consequence becomes ..
  100. pmc Sister-chromatid misbehavior in Drosophila ord mutants
    W Y Miyazaki
    Department of Biology, Massachusetts Institute of Technology, Cambridge
    Genetics 132:1047-61. 1992
    ..Cytological analysis in males confirmed that precocious disjunction of the sister chromatids occurs in prometaphase I...
  101. pmc Low molecular weight cyclin E overexpression shortens mitosis, leading to chromosome missegregation and centrosome amplification
    Rozita Bagheri-Yarmand
    Department of Experimental Radiation Oncology, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Cancer Res 70:5074-84. 2010
    ..First, the most significant difference is that EL overexpression prolongs cell cycle arrest in prometaphase, whereas LMW-E overexpression reduces the length of mitosis and accelerates mitotic exit...

Research Grants39

  1. Function of the chromosomal kinase haspin in mitosis
    JONATHAN M HIGGINS; Fiscal Year: 2010
    ..is first detected on chromosome arms in late G2/early prophase, becomes focused at centromeres by prometaphase, and declines during anaphase...
  2. CHROMOSOME MOVEMENT IN PROMETAPHASE
    Gary J Gorbsky; Fiscal Year: 2012
    ..The results from this study will also enlighten the mechanisms that contribute to chromosome imbalances that are the cause of many birth defects. ..
  3. Control of centrosome duplication and G1 arrest after prolonged prometaphase
    Greenfield Sluder; Fiscal Year: 2013
    ..provided by applicant): We will investigate the controls for centriole duplication and how prolonged prometaphase blocks daughter cell proliferation...
  4. ORGANIZATION OF THE MAMMALIAN MITOTIC SPINDLE
    Duane A Compton; Fiscal Year: 2013
    ..To use live cell imaging to determine the biochemical changes underlying the transition from prometaphase to metaphase;2...
  5. ILLEGITIMATE RECOMBINATION BY DRUG RESISTANCE ELEMENTS
    NANCY E KLECKNER; Fiscal Year: 2013
    ..coli versus the prophase-to-prometaphase transition of eukaryotic chromosomes...
  6. FORMATION,INTERACTION & FUNCTION OF SPINDLE COMPONENTS
    Conly L Rieder; Fiscal Year: 2010
    ..amp;length of astral microtubules, formed by centrosomes not associated with chromosomes, changes during prometaphase;b) that separating asters interact during prometaphase in the absence of associated chromosomes;c) that the ..
  7. Physical mapping of Aedes aegypti genome
    Maria Sharakhova; Fiscal Year: 2011
    ..We have recently discovered that prometaphase chromosomes from imaginal discs of 4th instar larvae can be an excellent source for the physical mapping of the ..
  8. Determining the spindle dynamics regulatory network with an integrated approach
    Ao Ma; Fiscal Year: 2013
    ..only a handful of MT regulatory proteins give rise to the broad range of dynamics at spindle MT plus-ends from prometaphase through anaphase...
  9. Control of Genomic Stability by Emi1 and Securin
    NORMAN LEHMAN; Fiscal Year: 2007
    ..Over-expression of Emi1 causes an abnormal prometaphase block and abnormal mitotic spindle formation...
  10. CHROMATIN MAPPING BY FLUORESCENCE IN SITU HYBRIDIZATION
    Barbara Trask; Fiscal Year: 1993
    ..3) To describe the relationship between known linear distance and measured interphase and prometaphase distances for sequences separated by 50 kbp to 3 Mbp...
  11. MOLECULAR ANALYSIS OF MICROTUBULE ORGANIZATION
    Harish Joshi; Fiscal Year: 2003
    ..pombe y-tubulin. Normally, abnormalities in spindle assembly arrest cells in prometaphase, and entry into anaphase and subsequent cytokinesis is inhibited...
  12. NUCLEAR ARCHITECTURAL CHANGES DURING FERTILIZATION
    Gerald Schatten; Fiscal Year: 1993
    ..kinetochore antibodies will prevent prometaphase congression and anaphase chromosome separation if introduced prior to metaphase; 3...
  13. Hsp90s as targets in the development of anticancer drugs
    Neal Rosen; Fiscal Year: 2004
    ..Cells with defective RB function arrest in prometaphase and undergo apoptosis. One ansamycin, 17 -allylaminogeeldanamycin (17- AAG) is currently in clinical trial...
  14. IMPROVED CHROMOSOME ANALYSIS VIA NEW SYNCHRONIZING AGENT
    FLOYD TAUB; Fiscal Year: 1993
    ..In addition, prometaphase spreads providing detailed banding may be more readily obtained...
  15. Role of Nuf2 in Kinetochore Function
    Jennifer DeLuca; Fiscal Year: 2004
    ..abstract_text> ..
  16. Molecular Structure and Function of the Human Kinetochore Outer Plate
    Bruce F McEwen; Fiscal Year: 2010
    ....
  17. ALL Fusion Proteins: Associated with Multi-protein Complexes and Role in Transcri
    Carlo M Croce; Fiscal Year: 2010
    ..abstract_text> ..
  18. Mechanisms of Microtubule Dynamic Turnover
    Patricia Wadsworth; Fiscal Year: 2009
    ..Learning how normal cells construct a mitotic spindle is an important first step towards understanding defective cell division in cancer. ..
  19. FHIT Gene Therapy in Cancer Prevention and Treatment
    Carlo M Croce; Fiscal Year: 2011
    ..abstract_text> ..
  20. 8P22 ALTERATIONS, IN PROSTATE, BREAST AND ESOPHAGEAL CA
    Carlo Croce; Fiscal Year: 2004
    ..We will also determine whether Fez1 loss of function makes cancer cells more resistant to chemotherapeutic drugs. Finally, we will determine whether FHIT and Fez1 are collaborating tumor suppressor genes ..
  21. MECHANISMS OF MITOTIC SPINDLE ASSEMBLY AND FUNCTION
    EDWARD SALMON; Fiscal Year: 2003
    ..Finally, we will analyze how MT dynamics may be involved in the regulation of cytokinesis. ..
  22. ARP--GENE TARGET OF THE ALL1 GENE IN ACUTE LEUKEMIAS
    Carlo Croce; Fiscal Year: 2002
    ..Acute lymphoblastic and acute myelogenous leukemias will be investigated for the expression, rearrangements and mutations of ALL-1 target genes. ..
  23. Purchase of Leica TCS SP2 Confocal Microscope
    Patricia Wadsworth; Fiscal Year: 2002
    ....
  24. ASSEMBLY AND TARGETING OF EPITHELIAL P-TYPE ATPASES
    Curtis Okamoto; Fiscal Year: 2001
    ....
  25. DELTAVISION RESTORATION MICROSCOPY SYSTEM MODEL 483
    Conly Rieder; Fiscal Year: 2001
    ..This Core will maintain the instrument and provide technical support to those Principal Investigators/Program Directors who are in need of low-light high- resolution 3-D fluorescent imaging. ..
  26. Mechanisms of Genetic Instability and Tumor Suppression
    GEOFFREY MYLES WAHL; Fiscal Year: 2010
    ..Only then can we develop therapies to target the genetic defects that disable P53 in a particular tumor. This proposal is designed to provide such information. ..
  27. Translational Control of Oxidative Stress in Myocardial Infarction
    Qin M Chen; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE: This grant proposes to study the mechanism of Nrf2 protein translation in cardiomyocytes and in the myocardium following oxidative stress. ..
  28. MOLECULAR GENETIC ANALYSIS OF MAMMALIAN DNA REPLICATION
    Geoffrey Wahl; Fiscal Year: 2004
    ....
  29. MOLECULAR MECHANISMS OF OXIDANT TOXICITY
    Qin Chen; Fiscal Year: 2004
    ..We have a unique finding of premature senescence with oxidative stress and will combine in vitro and in vivo approaches to uncover the trigger of unwanted effects of aging. ..
  30. Mitochondria distribution in cloned pig embryos
    Heide Schatten; Fiscal Year: 2005
    ..Pilot data from this RO3 small grants program research will be used to apply for funding through the RO1 mechanism. ..
  31. Cytoskeletal organization in apicomplexan parasites
    Heide Schatten; Fiscal Year: 2005
    ..Pilot data from this RO3 research will be used to apply for funding through the RO1 mechanism. ..
  32. Specification of Embryonic Stem Cells in Leech
    DANIEL SHAIN; Fiscal Year: 2005
    ..It is anticipated that LES cell-associated genes characterized in this study will have mammalian counterparts, based upon the conservation of developmentally important genes across metazoan phyla. ..
  33. MECHANISMS OF MICROTUBULE AND MITOTIC SPINDLE ASSEMBLY
    Lynne Cassimeris; Fiscal Year: 2006
    ..To address these issues, we will apply a number of fluorescence-based imaging methods combined with siRNA or injection of function-blocking proteins or antibodies. ..
  34. FLUORESCENT SPECKLE MICROSCOPY
    EDWARD SALMON; Fiscal Year: 2007
    ..Experience gained in the course of these studies will be used to direct and refine hardware and software development. [unreadable] [unreadable]..
  35. Steroid As Cytoprotectants against Oxidative Toxicity
    Qin Chen; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  36. Misregulation of apoptosis in cloned pig embryos
    Heide Schatten; Fiscal Year: 2007
    ..Pilot data from this R03 small grants program research will be used to apply for funding through the R01 mechanism. [unreadable] [unreadable] [unreadable]..
  37. The Role of Separase During Cytokinesis in C elegans
    Joshua Bembenek; Fiscal Year: 2008
    ..elegans. Through this analysis, I hope to extend the understanding of the molecular mechanisms utilized for the temporal coordination of chromosome segregation with the onset of anaphase and cytokinesis. ..
  38. Microfilament organization in mitosis and transformation
    Fumio Matsumura; Fiscal Year: 2009
    ..MYPT depletion delays the prometaphase/metaphase transition and causes cytokinesis failure...
  39. Mouse Models to Elucidate p53 Regulatory Mechanisms
    Geoffrey Wahl; Fiscal Year: 2007
    ..The data obtained and systems developed have relevance for p53-targeted therapies, and they provide a paradigm for how transcription factors convert complex inputs into distinct regulatory decisions. [unreadable] [unreadable]..