mitosis

Summary

Summary: A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.

Top Publications

  1. ncbi Requirement for p53 and p21 to sustain G2 arrest after DNA damage
    F Bunz
    The Howard Hughes Medical Institute and The Johns Hopkins Oncology Center, 424 North Bond Street, Baltimore, MD 21231, USA
    Science 282:1497-501. 1998
  2. pmc A quantitative atlas of mitotic phosphorylation
    Noah Dephoure
    Department of Cell Biology, Harvard University Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:10762-7. 2008
  3. pmc DNA breaks and chromosome pulverization from errors in mitosis
    Karen Crasta
    Department of Pediatric Oncology, Dana Farber Cancer Institute, 450 Brookline Avenue, Boston, Massachusetts 02115, USA
    Nature 482:53-8. 2012
  4. ncbi Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis
    Jesper V Olsen
    Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried near Munich, Germany
    Sci Signal 3:ra3. 2010
  5. pmc Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
    Beate Neumann
    MitoCheck Project Group, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nature 464:721-7. 2010
  6. pmc The chromosomal passenger complex (CPC): from easy rider to the godfather of mitosis
    Mar Carmena
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, ICB Michael Swann Building, King s Buildings Mayfield Road, Edinburgh EH9 3JR Scotland, UK
    Nat Rev Mol Cell Biol 13:789-803. 2012
  7. pmc Regulatory control of the resolution of DNA recombination intermediates during meiosis and mitosis
    Joao Matos
    London Research Institute, Cancer Research UK, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
    Cell 147:158-72. 2011
  8. ncbi 53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of chromosomes under replication stress
    Claudia Lukas
    Centre for Genotoxic Stress Research, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, DK 2100 Copenhagen, Denmark
    Nat Cell Biol 13:243-53. 2011
  9. pmc Loss of human Greatwall results in G2 arrest and multiple mitotic defects due to deregulation of the cyclin B-Cdc2/PP2A balance
    Andrew Burgess
    Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoleculaire, Centre National de la Recherche Scientifique UMR 5237, 34293 Montpellier Cedex 5, France
    Proc Natl Acad Sci U S A 107:12564-9. 2010
  10. pmc The centrosome cycle: Centriole biogenesis, duplication and inherent asymmetries
    Erich A Nigg
    Biozentrum, University of Basel, Klingelbergstrasse 50 70, CH 4056 Basel, Switzerland
    Nat Cell Biol 13:1154-60. 2011

Detail Information

Publications361 found, 100 shown here

  1. ncbi Requirement for p53 and p21 to sustain G2 arrest after DNA damage
    F Bunz
    The Howard Hughes Medical Institute and The Johns Hopkins Oncology Center, 424 North Bond Street, Baltimore, MD 21231, USA
    Science 282:1497-501. 1998
    ..After disruption of either the p53 or the p21 gene, gamma radiated cells progressed into mitosis and exhibited a G2 DNA content only because of a failure of cytokinesis...
  2. pmc A quantitative atlas of mitotic phosphorylation
    Noah Dephoure
    Department of Cell Biology, Harvard University Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:10762-7. 2008
    ..We observed >1,000 proteins with increased phosphorylation in mitosis including many known cell cycle regulators...
  3. pmc DNA breaks and chromosome pulverization from errors in mitosis
    Karen Crasta
    Department of Pediatric Oncology, Dana Farber Cancer Institute, 450 Brookline Avenue, Boston, Massachusetts 02115, USA
    Nature 482:53-8. 2012
    ..Pulverization of chromosomes in micronuclei may also be one explanation for 'chromothripsis' in cancer and developmental disorders, where isolated chromosomes or chromosome arms undergo massive local DNA breakage and rearrangement...
  4. ncbi Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis
    Jesper V Olsen
    Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried near Munich, Germany
    Sci Signal 3:ra3. 2010
    ..In particular, nuclear proteins and proteins involved in regulating metabolic processes have high phosphorylation site occupancy in mitosis. This suggests that these proteins may be inactivated by phosphorylation in mitotic cells.
  5. pmc Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
    Beate Neumann
    MitoCheck Project Group, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nature 464:721-7. 2010
    ..As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community...
  6. pmc The chromosomal passenger complex (CPC): from easy rider to the godfather of mitosis
    Mar Carmena
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, ICB Michael Swann Building, King s Buildings Mayfield Road, Edinburgh EH9 3JR Scotland, UK
    Nat Rev Mol Cell Biol 13:789-803. 2012
    ..This complex targets to different locations at differing times during mitosis, where it regulates key mitotic events: correction of chromosome-microtubule attachment errors; activation of the ..
  7. pmc Regulatory control of the resolution of DNA recombination intermediates during meiosis and mitosis
    Joao Matos
    London Research Institute, Cancer Research UK, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
    Cell 147:158-72. 2011
    ..Here, we show that the specialized chromosome segregation patterns of meiosis and mitosis, which require the coordination of recombination with cell-cycle progression, are achieved by regulating the ..
  8. ncbi 53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of chromosomes under replication stress
    Claudia Lukas
    Centre for Genotoxic Stress Research, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, DK 2100 Copenhagen, Denmark
    Nat Cell Biol 13:243-53. 2011
    ..Together, these data indicate that restoration of DNA or chromatin integrity at loci prone to replication problems requires mitotic transmission to the next cell generations...
  9. pmc Loss of human Greatwall results in G2 arrest and multiple mitotic defects due to deregulation of the cyclin B-Cdc2/PP2A balance
    Andrew Burgess
    Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoleculaire, Centre National de la Recherche Scientifique UMR 5237, 34293 Montpellier Cedex 5, France
    Proc Natl Acad Sci U S A 107:12564-9. 2010
    ..When the levels of this kinase are only partially depleted, however, cells enter into mitosis with multiple defects and fail to inactivate the spindle assembly checkpoint (SAC)...
  10. pmc The centrosome cycle: Centriole biogenesis, duplication and inherent asymmetries
    Erich A Nigg
    Biozentrum, University of Basel, Klingelbergstrasse 50 70, CH 4056 Basel, Switzerland
    Nat Cell Biol 13:1154-60. 2011
    ..We discuss spatial aspects of the centrosome duplication cycle, the mechanism of centriole assembly and the possible consequences of the inherent asymmetry of centrioles and centrosomes...
  11. ncbi Regulation of the G2/M transition by p53
    W R Taylor
    Department of Molecular Biology, Lerner Research Insititute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
    Oncogene 20:1803-15. 2001
    ..Here we review recent evidence that implicates p53 in controlling entry into mitosis when cells enter G2 with damaged DNA or when they are arrested in S phase due to depletion of the substrates ..
  12. ncbi Microtubule assembly during mitosis - from distinct origins to distinct functions?
    Sylvain Meunier
    Microtubule Function and Cell Division group, Cell and Developmental Biology Program, Centre for Genomic Regulation CRG and UPF, Dr Aiguader 88, 08003 Barcelona, Spain
    J Cell Sci 125:2805-14. 2012
    ..In this Commentary, we review the recent advances in the field and discuss how different MT assembly pathways might cooperate to successfully form the mitotic spindle...
  13. ncbi Mitotic catastrophe: a mechanism for avoiding genomic instability
    Ilio Vitale
    Institut National de la Sante et de la Recherche Medicale INSERM, Villejuif, France
    Nat Rev Mol Cell Biol 12:385-92. 2011
    The improper distribution of chromosomes during mitosis compromises cellular functions and can reduce cellular fitness or contribute to malignant transformation...
  14. pmc The double bromodomain protein Brd4 binds to acetylated chromatin during interphase and mitosis
    Anup Dey
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 100:8758-63. 2003
    ..This colocalization also required both bromodomains. These observations indicate that Brd4 specifically recognizes acetylated histone codes, and this recognition is passed onto the chromatin of newly divided cells...
  15. pmc Chk1 is an essential kinase that is regulated by Atr and required for the G(2)/M DNA damage checkpoint
    Q Liu
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Genes Dev 14:1448-59. 2000
    ..Taken together, these data indicate that Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development, and tumor suppression, and that Atr regulates Chk1...
  16. ncbi Un ménage à quatre: the molecular biology of chromosome segregation in meiosis
    Mark Petronczki
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, 1030 Vienna, Austria
    Cell 112:423-40. 2003
    ..Whereas mitosis produces diploid daughter cells from diploid cells, meiosis generates haploid gametes from diploid precursors...
  17. pmc Structured illumination of the interface between centriole and peri-centriolar material
    Jingyan Fu
    Cancer Research UK Cell Cycle Genetics Group, Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK
    Open Biol 2:120104. 2012
    The increase in centrosome size in mitosis was described over a century ago, and yet it is poorly understood how centrioles, which lie at the core of centrosomes, organize the pericentriolar material (PCM) in this process...
  18. ncbi Systematic genetic analysis with ordered arrays of yeast deletion mutants
    A H Tong
    Banting and Best Department of Medical Research, University of Toronto, Toronto ON, Canada M5G 1L6
    Science 294:2364-8. 2001
    ..Systematic application of this approach should produce a global map of gene function...
  19. ncbi Mitotic kinases as regulators of cell division and its checkpoints
    E A Nigg
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Nat Rev Mol Cell Biol 2:21-32. 2001
    b>Mitosis and cytokinesis are undoubtedly the most spectacular parts of the cell cycle. Errors in the choreography of these processes can lead to aneuploidy or genetic instability, fostering cell death or disease...
  20. pmc Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosis
    Olivier Gavet
    The Gurdon Institute and Department of Zoology, Tennis Court Road, CB2 1QN Cambridge, UK
    Dev Cell 18:533-43. 2010
    The CyclinB1-Cdk1 kinase is the catalytic activity at the heart of mitosis-promoting factor (MPF), yet fundamental questions concerning its role in mitosis remained unresolved...
  21. ncbi Cell death by mitotic catastrophe: a molecular definition
    Maria Castedo
    CNRS UMR 8125, Institut Gustave Roussy, Pavillon de Recherche 1, 39 rue Camille Desmoulins, Villejuif F 94805, France
    Oncogene 23:2825-37. 2004
    ..literature is devoid of a clearcut definition of mitotic catastrophe, a type of cell death that occurs during mitosis. Here, we propose that mitotic catastrophe results from a combination of deficient cell-cycle checkpoints (in ..
  22. ncbi Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis
    T Lee
    Department of Biological Sciences, Stanford University, California 94305, USA
    Neuron 22:451-61. 1999
    ..As an example, we show that the short stop gene is required in mushroom body neurons for the extension and guidance of their axons...
  23. pmc Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation
    Tomomi Kiyomitsu
    Whitehead Institute, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Cell Biol 14:311-7. 2012
    ..We propose that these chromosome- and spindle-pole-derived gradients generate an intrinsic code to control spindle position and orientation...
  24. ncbi Control of apoptosis and mitotic spindle checkpoint by survivin
    F Li
    Boyer Center for Molecular Medicine, Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Nature 396:580-4. 1998
    ..At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is ..
  25. ncbi Shared and separate functions of polo-like kinases and aurora kinases in cancer
    Susanne M A Lens
    Department of Medical Oncology and Cancer Genomics Centre, UMC Utrecht, Universiteitsweg 100, Stratenum 2 118, Utrecht 3584 CG, The Netherlands
    Nat Rev Cancer 10:825-41. 2010
    ..Interest in these drugs is fuelled by the idea that these kinases have unique functions in mitosis. Within the polo-like kinase family, the emphasis for targeted therapies has been on polo-like kinase 1 (PLK1), ..
  26. pmc The decision to enter mitosis: feedback and redundancy in the mitotic entry network
    Arne Lindqvist
    Department of Medical Oncology, University Medical Center Utrecht, 3584 CG Utrecht, Netherlands
    J Cell Biol 185:193-202. 2009
    The decision to enter mitosis is mediated by a network of proteins that regulate activation of the cyclin B-Cdk1 complex...
  27. ncbi The double bromodomain-containing chromatin adaptor Brd4 and transcriptional regulation
    Shwu Yuan Wu
    Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA
    J Biol Chem 282:13141-5. 2007
    ..mediated by Brd4 interaction with virus-encoded E2 protein, facilitates viral genome segregation during mitosis. Interestingly, Brd4 is also identified in a transcriptional silencing complex assembled by HPV E2 and turns out ..
  28. pmc Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1
    Lyubomir T Vassilev
    Departments of Discovery Oncology and Discovery Chemistry, Roche Research Center, Hoffmann La Roche, Inc, Nutley, NJ 07110, USA
    Proc Natl Acad Sci U S A 103:10660-5. 2006
    CDK1 is a nonredundant cyclin-dependent kinase (CDK) with an essential role in mitosis, but its multiple functions still are poorly understood at a molecular level...
  29. ncbi Cancer cells display profound intra- and interline variation following prolonged exposure to antimitotic drugs
    Karen E Gascoigne
    Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK
    Cancer Cell 14:111-22. 2008
    ..We present data supporting a model wherein cell fate is dictated by two competing networks, one involving caspase activation, the other protecting cyclin B1 from degradation...
  30. ncbi Mitotic chromosomal instability and cancer: mouse modelling of the human disease
    Juan Manuel Schvartzman
    Program in Cancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Nat Rev Cancer 10:102-15. 2010
    ..instability - the inability to faithfully segregate equal chromosome complements to two daughter cells during mitosis - is a widespread phenomenon in solid tumours that is thought to serve as the fuel for tumorigenic progression...
  31. ncbi Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle
    Henrik Daub
    Cell Signaling Group, Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 31:438-48. 2008
    ..Intriguingly, half of all kinase phosphopeptides were upregulated in mitosis. Our data reveal numerous unknown M phase-induced phosphorylation sites on kinases with established mitotic ..
  32. pmc Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine
    Stefano Santaguida
    Department of Experimental Oncology, European Institute of Oncology, I 20139 Milan, Italy
    J Cell Biol 190:73-87. 2010
    ..They also shed light on the relationship between the error correction pathway and the spindle checkpoint and suggest that these processes are coregulated and are likely to share at least a subset of their catalytic machinery...
  33. pmc The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics
    Shinya Ohta
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Cell 142:810-21. 2010
    ..Our integrated analysis predicts that up to 97 new centromere-associated proteins remain to be discovered in our data set...
  34. ncbi Flies without centrioles
    Renata Basto
    The Gurdon Institute, Tennis Court Road, Cambridge CB2 1QN, UK
    Cell 125:1375-86. 2006
    ..Thus, centrioles are essential for the formation of centrosomes, cilia, and flagella, but, remarkably, they are not essential for most aspects of Drosophila development...
  35. pmc Systematic analysis of human protein complexes identifies chromosome segregation proteins
    James R A Hutchins
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Science 328:593-9. 2010
    ..The approaches we describe here are generally applicable to high-throughput follow-up analyses of phenotypic screens in mammalian cells...
  36. pmc Asymmetric cell divisions promote stratification and differentiation of mammalian skin
    Terry Lechler
    Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    Nature 437:275-80. 2005
    ..We further demonstrate that integrins and cadherins are essential for the apical localization of atypical protein kinase C, the Par3-LGN-Inscuteable complex and NuMA-dynactin to align the spindle...
  37. ncbi Greatwall phosphorylates an inhibitor of protein phosphatase 2A that is essential for mitosis
    Satoru Mochida
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
    Science 330:1670-3. 2010
    Entry into mitosis in eukaryotes requires the activity of cyclin-dependent kinase 1 (Cdk1)...
  38. pmc Gene bookmarking accelerates the kinetics of post-mitotic transcriptional re-activation
    Rui Zhao
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Cell Biol 13:1295-304. 2011
    ..Using a real-time quantitative approach, we identified differences in the kinetics of transcriptional activation between interphase and post-mitotic cells that are mediated by a chromatin-based epigenetic mechanism...
  39. ncbi The substrate of Greatwall kinase, Arpp19, controls mitosis by inhibiting protein phosphatase 2A
    Aicha Gharbi-Ayachi
    Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoleculaire, CNRS UMR 5237, IFR 122, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    Science 330:1673-7. 2010
    Initiation and maintenance of mitosis require the activation of protein kinase cyclin B-Cdc2 and the inhibition of protein phosphatase 2A (PP2A), which, respectively, phosphorylate and dephosphorylate mitotic substrates...
  40. ncbi Death through a tragedy: mitotic catastrophe
    H Vakifahmetoglu
    Division of Toxicology, Institute of Environmental Medicine, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Cell Death Differ 15:1153-62. 2008
    ..has long been considered as a mode of cell death that results from premature or inappropriate entry of cells into mitosis and can be caused by chemical or physical stresses...
  41. pmc Forkhead box M1 regulates the transcriptional network of genes essential for mitotic progression and genes encoding the SCF (Skp2-Cks1) ubiquitin ligase
    I Ching Wang
    Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, College of Medicine, 60607 7170, USA
    Mol Cell Biol 25:10875-94. 2005
    ..Taken together, these results demonstrate that FoxM1 regulates transcription of cell cycle genes critical for progression into S-phase and mitosis.
  42. pmc Induced ectopic kinetochore assembly bypasses the requirement for CENP-A nucleosomes
    Karen E Gascoigne
    Whitehead Institute for Biomedical Research, Department of Biology, Nine Cambridge Center, MA 02142, USA
    Cell 145:410-22. 2011
    ..We additionally find that CENP-T phosphorylation regulates the mitotic assembly of both endogenous and ectopic kinetochores. Thus, CENP-C and CENP-T form a critical regulated platform for vertebrate kinetochore assembly...
  43. ncbi Survivin: key regulator of mitosis and apoptosis and novel target for cancer therapeutics
    Alain C Mita
    Institute for Drug Development, Cancer Therapy and Research Center at the University of Texas Health Science Center, 7979 Wurzbach Road, San Antonio, TX 78229, USA
    Clin Cancer Res 14:5000-5. 2008
    Survivin, a member of the family of inhibitor of apoptosis proteins, functions as a key regulator of mitosis and programmed cell death. Initially, survivin was described as an inhibitor of caspase-9...
  44. pmc Mus81 and Yen1 promote reciprocal exchange during mitotic recombination to maintain genome integrity in budding yeast
    Chu Kwen Ho
    Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY 10032, USA
    Mol Cell 40:988-1000. 2010
    ....
  45. pmc Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis
    J Mendez
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Mol Cell Biol 20:8602-12. 2000
    ..throughout the cell cycle, hCdc6p levels vary, being very low in early G(1) and accumulating until cells enter mitosis. hCdc6p can be polyubiquitinated in vivo, and it is stabilized by proteasome inhibitors...
  46. pmc Friedreich's ataxia (GAA)n•(TTC)n repeats strongly stimulate mitotic crossovers in Saccharomyces cerevisae
    Wei Tang
    Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, United States of America
    PLoS Genet 7:e1001270. 2011
    ..These findings indicate that (GAA)(n)•(TTC)(n) tracts can be a potent source of loss of heterozygosity in yeast...
  47. ncbi Inhibitors targeting mitosis: tales of how great drugs against a promising target were brought down by a flawed rationale
    Edina Komlodi-Pasztor
    Medical Oncology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 1906, USA
    Clin Cancer Res 18:51-63. 2012
    Although they have been advocated with an understandable enthusiasm, mitosis-specific agents such as inhibitors of mitotic kinases and kinesin spindle protein have not been successful clinically...
  48. ncbi Studies of nuclear and cytoplasmic behaviour during the five mitotic cycles that precede gastrulation in Drosophila embryogenesis
    V E Foe
    J Cell Sci 61:31-70. 1983
    ..10 through 13, it is commonly observed in living embryos that the syncytial blastoderm nuclei enter (and leave) mitosis in one of two waves that originate nearly simultaneously from the opposite anterior and posterior poles of the ..
  49. ncbi A conserved checkpoint pathway mediates DNA damage--induced apoptosis and cell cycle arrest in C. elegans
    A Gartner
    Cold Spring Harbor Laboratory, New York 11724, USA
    Mol Cell 5:435-43. 2000
    ..elegans homolog of the S. pombe rad1 checkpoint gene, rad-5, and him-7-block both DNA damage-induced apoptosis and cell proliferation arrest. Our results implicate rad1 homologs in DNA damage-induced apoptosis in animals...
  50. pmc Identification of cis-acting sites for condensin loading onto budding yeast chromosomes
    Claudio D'Ambrosio
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
    Genes Dev 22:2215-27. 2008
    Eukaryotic chromosomes reach their stable rod-shaped appearance in mitosis in a reaction dependent on the evolutionarily conserved condensin complex. Little is known about how and where condensin associates with chromosomes...
  51. ncbi Coordination of kinase and phosphatase activities by Lem4 enables nuclear envelope reassembly during mitosis
    Claudio Asencio
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Cell 150:122-35. 2012
    b>Mitosis in metazoa requires nuclear envelope (NE) disassembly and reassembly. NE disassembly is driven by multiple phosphorylation events...
  52. ncbi Polo on the Rise-from Mitotic Entry to Cytokinesis with Plk1
    Mark Petronczki
    Cell Division and Aneuploidy Laboratory, Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire, EN6 3LD, United Kingdom
    Dev Cell 14:646-59. 2008
    ..In particular, we highlight new work on the role of Plk1 in cytokinesis in human cells. Finally, we describe how better understanding of Plk1 functions allows critical evaluation of Plk1 as a potential drug target for cancer therapy...
  53. ncbi Aneuploidy and cancer
    Harith Rajagopalan
    The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, 1650 Orleans Street, Baltimore, Maryland 21231, USA
    Nature 432:338-41. 2004
    ..A better understanding of the molecular mechanisms leading to aneuploidy holds promise for the development of cancer drugs that target this process...
  54. pmc Histone H3 Thr-3 phosphorylation by Haspin positions Aurora B at centromeres in mitosis
    Fangwei Wang
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Harvard Medical School, Smith Building, 1 Jimmy Fund Way, Boston, MA 02115, USA
    Science 330:231-5. 2010
    ..Therefore, H3T3ph generated by Haspin positions the CPC at centromeres to regulate selected targets of Aurora B during mitosis.
  55. ncbi Two histone marks establish the inner centromere and chromosome bi-orientation
    Yuya Yamagishi
    Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113 0032, Japan
    Science 330:239-43. 2010
    For proper partitioning of chromosomes in mitosis, the chromosomal passenger complex (CPC) including Aurora B and survivin must be localized at the center of paired kinetochores, at the site called the inner centromere...
  56. pmc Survivin reads phosphorylated histone H3 threonine 3 to activate the mitotic kinase Aurora B
    Alexander E Kelly
    Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065, USA
    Science 330:235-9. 2010
    A hallmark of mitosis is the appearance of high levels of histone phosphorylation, yet the roles of these modifications remain largely unknown...
  57. pmc Megabase chromatin domains involved in DNA double-strand breaks in vivo
    E P Rogakou
    Laboratory of Molecular Pharmacology, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Biol 146:905-16. 1999
    ..The results further suggest the possible existence of units of higher order chromatin structure involved in monitoring DNA integrity...
  58. pmc Multipolar spindle pole coalescence is a major source of kinetochore mis-attachment and chromosome mis-segregation in cancer cells
    William T Silkworth
    Virginia Tech, Department of Biological Sciences, Blacksburg, VA, USA
    PLoS ONE 4:e6564. 2009
    ..We found that CIN cells frequently assembled multipolar spindles in early mitosis. However, multipolar anaphase cells were very rare, and live-cell experiments showed that almost all CIN cells ..
  59. pmc Dividing cellular asymmetry: asymmetric cell division and its implications for stem cells and cancer
    Ralph A Neumüller
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, 1030 Vienna, Austria
    Genes Dev 23:2675-99. 2009
    ..We summarize the relevance of asymmetric cell divisions in various stem cell systems and discuss why defects in asymmetric cell division can lead to the formation of tumors...
  60. pmc Bicaudal D2, dynein, and kinesin-1 associate with nuclear pore complexes and regulate centrosome and nuclear positioning during mitotic entry
    Daniël Splinter
    Department of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands
    PLoS Biol 8:e1000350. 2010
    ....
  61. ncbi Mitotic phosphorylation of dynamin-related GTPase Drp1 participates in mitochondrial fission
    Naoko Taguchi
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 282:11521-9. 2007
    Organelles are inherited to daughter cells beyond dynamic changes of the membrane structure during mitosis. Mitochondria are dynamic entities, frequently dividing and fusing with each other, during which dynamin-related GTPase Drp1 is ..
  62. ncbi Cubism and the cell cycle: the many faces of the APC/C
    Jonathon Pines
    The Gurdon Institute, Tennis Court Road, Cambridge CB2 1QN, UK
    Nat Rev Mol Cell Biol 12:427-38. 2011
    ..cells receive an equal and identical set of chromosomes from their mother and that DNA replication always follows mitosis. Remarkably, one protein complex is responsible for this surgical precision, the APC/C (anaphase-promoting ..
  63. ncbi Hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding
    Martin P Stewart
    ETH Zurich, Department of Biosystems Science and Engineering, CH 4058 Basel, Switzerland
    Nature 469:226-30. 2011
    During mitosis, adherent animal cells undergo a drastic shape change, from essentially flat to round...
  64. ncbi Microtubule-organizing centres: a re-evaluation
    Jens Lüders
    Department of Biological Sciences, Stanford University, CA 94305, USA
    Nat Rev Mol Cell Biol 8:161-7. 2007
    ..These findings suggest that the spatio-temporal control of microtubule nucleation is more complex than previously thought, leading us to a re-evaluation of the concept of the microtubule-organizing center...
  65. pmc p53-deficient cells rely on ATM- and ATR-mediated checkpoint signaling through the p38MAPK/MK2 pathway for survival after DNA damage
    H Christian Reinhardt
    Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02139, USA
    Cancer Cell 11:175-89. 2007
    ..We show that the Chk1 inhibitor UCN-01 also potently inhibits MK2, suggesting that its clinical efficacy results from the simultaneous disruption of two critical checkpoint pathways in p53-defective cells...
  66. pmc PKM2 regulates chromosome segregation and mitosis progression of tumor cells
    Yuhui Jiang
    Brain Tumor Center and Department of Neuro Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Mol Cell 53:75-87. 2014
    ..We show here that PKM2, but not PKM1, binds to the spindle checkpoint protein Bub3 during mitosis and phosphorylates Bub3 at Y207...
  67. ncbi Orchestrating nuclear envelope disassembly and reassembly during mitosis
    Stephan Güttinger
    Institute of Biochemistry, Eidgenossische Technische Hochschule Zurich, HPM F11 1, Schafmattstrasse 18, 8093 Zurich, Switzerland
    Nat Rev Mol Cell Biol 10:178-91. 2009
    ..Higher eukaryotic cells have evolved 'open' mitosis, the most extreme mechanism to solve the problem of nuclear division, in which the NE is initially completely ..
  68. pmc Contrasting roles of condensin I and condensin II in mitotic chromosome formation
    Lydia C Green
    Murdoch Childrens Research Institute, Royal Children s Hospital, Melbourne, Victoria 3052, Australia
    J Cell Sci 125:1591-604. 2012
    ..To dissect accurately the role of each complex in mitosis, we have made and studied the first vertebrate conditional knockouts of the genes encoding condensin I subunit ..
  69. pmc Mechanisms of chromosomal instability
    Sarah L Thompson
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Curr Biol 20:R285-95. 2010
    ..Understanding these mechanisms provides insight into the cellular consequences of CIN and reveals the possibility of exploiting CIN in cancer therapy...
  70. ncbi Connecting up and clearing out: how kinetochore attachment silences the spindle assembly checkpoint
    Geert J P L Kops
    Department of Medical Oncology, University Medical Center Utrecht, Utrecht, The Netherlands
    Chromosoma 121:509-25. 2012
    ..but productive attachment of all kinetochores is required to satisfy the SAC, initiate anaphase, and exit from mitosis. Although mechanisms of kinetochore signaling and SAC inhibitor assembly and function have received the bulk of ..
  71. pmc The kinetochore-bound Ska1 complex tracks depolymerizing microtubules and binds to curved protofilaments
    Jens C Schmidt
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Dev Cell 23:968-80. 2012
    To ensure equal chromosome segregation during mitosis, the macromolecular kinetochore must remain attached to depolymerizing microtubules, which drive chromosome movements...
  72. ncbi Geminin, an inhibitor of DNA replication, is degraded during mitosis
    T J McGarry
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 93:1043-53. 1998
    ..We propose that geminin inhibits DNA replication during S, G2, and M phases and that geminin destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle...
  73. ncbi Aurora-A - a guardian of poles
    Tomotoshi Marumoto
    Department of Tumor Genetics and Biology, Graduate School of Medical Sciences, Kumamoto University, 1 1 1 Honjo, Kumamoto 860 8556, Japan
    Nat Rev Cancer 5:42-50. 2005
    ..Aurora-A is mainly localized at spindle poles and the mitotic spindle during mitosis, where it regulates the functions of centrosomes, spindles and kinetochores required for proper mitotic ..
  74. pmc High-resolution genome-wide analysis of irradiated (UV and γ-rays) diploid yeast cells reveals a high frequency of genomic loss of heterozygosity (LOH) events
    Jordan St Charles
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Genetics 190:1267-84. 2012
    ..To our knowledge, our study represents the first high-resolution genome-wide analysis of DNA damage-induced LOH events performed in any eukaryote...
  75. ncbi External forces control mitotic spindle positioning
    Jenny Fink
    Institut Curie, CNRS UMR 144, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Nat Cell Biol 13:771-8. 2011
    ..We found that forces applied on the cell body direct spindle orientation during mitosis. We further show that external constraints induce a polarization of dynamic subcortical actin structures that ..
  76. ncbi SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box
    C Bai
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Cell 86:263-74. 1996
    ..Different skp1 mutants arrest cells in either G1 or G2, suggesting a connection between regulation of proteolysis in different stages of the cycle...
  77. pmc Live-cell imaging RNAi screen identifies PP2A-B55alpha and importin-beta1 as key mitotic exit regulators in human cells
    Michael H A Schmitz
    Institute of Biochemistry, Swiss Federal Institute of Technology Zurich ETHZ, Schafmattstrasse 18, CH 8093 Zurich, Switzerland
    Nat Cell Biol 12:886-93. 2010
    When vertebrate cells exit mitosis various cellular structures are re-organized to build functional interphase cells. This depends on Cdk1 (cyclin dependent kinase 1) inactivation and subsequent dephosphorylation of its substrates...
  78. ncbi A spatial gradient coordinates cell size and mitotic entry in fission yeast
    James B Moseley
    The Rockefeller University, New York, New York 10065, USA
    Nature 459:857-60. 2009
    ..In the fission yeast Schizosaccharomyces pombe, cells enter mitosis and divide at a defined and reproducible size owing to the regulated activity of Cdk1 (refs 2, 3)...
  79. pmc Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesis
    Wulf Haubensak
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 101:3196-201. 2004
    ..Our observations reconcile previous data on the origin and lineage of CNS neurons and show that basal, rather than apical, progenitors are the major source of the neurons of the mammalian neocortex...
  80. ncbi The Aurora kinase family in cell division and cancer
    Gerben Vader
    Laboratory of Experimental Oncology, Department of Medical Oncology, University Medical Center Utrecht, Stratenum 2 125, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
    Biochim Biophys Acta 1786:60-72. 2008
    ....
  81. pmc The Ndc80 kinetochore complex forms oligomeric arrays along microtubules
    Gregory M Alushin
    Biophysics Graduate Group, University of California, Berkeley, California 94720, USA
    Nature 467:805-10. 2010
    ..The complex's mode of interaction with the microtubule and its oligomerization suggest a mechanism by which Aurora B could regulate the stability of load-bearing kinetochore-microtubule attachments...
  82. ncbi Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells
    Takaki Miyata
    Laboratory for Cell Culture Development, Brain Science Institute, RIKEN, Saitama 351 0198, Japan
    Development 131:3133-45. 2004
    ..The asymmetric P/P division may therefore contribute to efficient neuron/progenitor segregation required for CP initiation through cell cycle-dependent and lineage-restricted expression of Ngn2...
  83. pmc A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers
    J R Bischoff
    SUGEN, Inc, Redwood City, California 94063, USA
    EMBO J 17:3052-65. 1998
    ..These observations implicate aurora2 as a potential oncogene in many colon, breast and other solid tumors, and identify centrosome-associated proteins as novel targets for cancer therapy...
  84. pmc A super-resolution map of the vertebrate kinetochore
    Susana Abreu Ribeiro
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Proc Natl Acad Sci U S A 107:10484-9. 2010
    ..In mitosis, a CENP-C-dependent mechanism crosslinks CENP-A blocks of different layers together, conferring extra stability ..
  85. ncbi Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery
    Libor Macurek
    Department of Medical Oncology, University Medical Center Utrecht, Utrecht 3584CG, The Netherlands
    Nature 455:119-23. 2008
    ..Here we show that in human cells PLK1 activation occurs several hours before entry into mitosis, and requires aurora A (AURKA, also known as STK6)-dependent phosphorylation of Thr 210...
  86. pmc The cortical protein Lte1 promotes mitotic exit by inhibiting the spindle position checkpoint kinase Kin4
    Daniela Trinca Bertazzi
    DKFZ ZMBH Alliance, German Cancer Research Center, 69120 Heidelberg, Germany
    J Cell Biol 193:1033-48. 2011
    ..This Lte1-mediated exclusion of Kin4 from the dSPB is essential for proper mitotic exit of cells with a correctly aligned spindle. Therefore, Lte1 promotes mitotic exit by inhibiting Kin4 activity at the dSPB...
  87. pmc Regulation of APC/C activators in mitosis and meiosis
    Jillian A Pesin
    Whitehead Institute and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Annu Rev Cell Dev Biol 24:475-99. 2008
    ..APC/C) is a multisubunit E3 ubiquitin ligase that triggers the degradation of multiple substrates during mitosis. Cdc20/Fizzy and Cdh1/Fizzy-related activate the APC/C and confer substrate specificity through complex ..
  88. pmc Loss of pRB causes centromere dysfunction and chromosomal instability
    Amity L Manning
    Massachusetts General Hospital Cancer Center and Harvard Medical School, Charlestown, Massachusetts 02129, USA
    Genes Dev 24:1364-76. 2010
    ..consequences, these more moderate defects allow pRB-deficient cells to proliferate but undermine the fidelity of mitosis, leading to whole-chromosome gains and losses...
  89. ncbi CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging
    Michael Held
    Institute of Biochemistry, Swiss Federal Institute of Technology Zurich, Zurich, Switzerland
    Nat Methods 7:747-54. 2010
    ..CellCognition is published as open source software, enabling live-cell imaging-based screening with assays that directly score cellular dynamics...
  90. ncbi Polar gradients of the DYRK-family kinase Pom1 couple cell length with the cell cycle
    Sophie G Martin
    Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Genopode Building, 1015 Lausanne, Switzerland
    Nature 459:852-6. 2009
    Cells normally grow to a certain size before they enter mitosis and divide. Entry into mitosis depends on the activity of Cdk1, which is inhibited by the Wee1 kinase and activated by the Cdc25 phosphatase...
  91. pmc Making the Auroras glow: regulation of Aurora A and B kinase function by interacting proteins
    Mar Carmena
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Michael Swann Building, King s Buildings, Edinburgh, Scotland, UK
    Curr Opin Cell Biol 21:796-805. 2009
    The conserved Aurora family of protein kinases have emerged as crucial regulators of mitosis and cytokinesis...
  92. pmc Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2
    Silke Hauf
    Research Institute of Molecular Pathology, Vienna, Austria
    PLoS Biol 3:e69. 2005
    ..We have identified numerous mitosis-specific sites on Scc1 and SA2, mutated them, and expressed nonphosphorylatable forms of both proteins stably at ..
  93. ncbi Replication stress induces sister-chromatid bridging at fragile site loci in mitosis
    Kok Lung Chan
    Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DS, UK
    Nat Cell Biol 11:753-60. 2009
    ..these loci are frequently interlinked through BLM-associated ultra-fine DNA bridges (UFBs) even as cells traverse mitosis. Similarly to fragile site expression, fragile site bridging is induced after partial inhibition of DNA ..
  94. pmc Plk1-dependent phosphorylation of FoxM1 regulates a transcriptional programme required for mitotic progression
    Zheng Fu
    Department of Urology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    Nat Cell Biol 10:1076-82. 2008
    Proper control of entry into and progression through mitosis is essential for normal cell proliferation and the maintenance of genome stability...
  95. pmc Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage
    Xing Zeng
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 18:382-95. 2010
    ..We propose that mutual antagonism between the APC and the SAC yields a positive feedback loop that amplifies the ability of TAME to induce mitotic arrest...
  96. pmc SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation
    Vincenzo D'Angiolella
    Department of Pathology, NYU Cancer Institute, New York University School of Medicine, 522 First Avenue, SRB 1107, New York, New York 10016, USA
    Nature 466:138-42. 2010
    ..We propose that SCF(Cyclin F)-mediated degradation of CP110 is required for the fidelity of mitosis and genome integrity.
  97. pmc RhoA GTPase is dispensable for actomyosin regulation but is essential for mitosis in primary mouse embryonic fibroblasts
    Jaime Melendez
    Division of Experimental Hematology and Cancer Biology, Children s Hospital Research Foundation, University of Cincinnati, Cincinnati, Ohio 45229, USA
    J Biol Chem 286:15132-7. 2011
    ..Proliferation of RhoA(-/-) cells was impaired due to a complete cell cycle block during mitosis, an effect that is associated with defective cytokinesis and chromosome segregation and can be readily rescued by ..
  98. pmc A positive feedback loop involving Haspin and Aurora B promotes CPC accumulation at centromeres in mitosis
    Fangwei Wang
    Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Curr Biol 21:1061-9. 2011
    Haspin phosphorylates histone H3 at Thr3 (H3T3ph) during mitosis [1, 2], providing a chromatin binding site for the chromosomal passenger complex (CPC) at centromeres to regulate chromosome segregation [3-5]...
  99. ncbi Phosphatases: providing safe passage through mitotic exit
    Claudia Wurzenberger
    Institute of Biochemistry, Department of Biology, Swiss Federal Institute of Technology Zurich ETHZ, HPM D11 3, Schafmattstrasse 18, 8093 Zurich, Switzerland
    Nat Rev Mol Cell Biol 12:469-82. 2011
    The mitosis-to-interphase transition involves dramatic cellular reorganization from a state that supports chromosome segregation to a state that complies with all functions of an interphase cell...
  100. pmc Switches and latches: a biochemical tug-of-war between the kinases and phosphatases that control mitosis
    Maria Rosa Domingo-Sananes
    Department of Biochemistry, Oxford Centre for Integrative Systems Biology, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Philos Trans R Soc Lond B Biol Sci 366:3584-94. 2011
    Activation of the cyclin-dependent kinase (Cdk1) cyclin B (CycB) complex (Cdk1:CycB) in mitosis brings about a remarkable extent of protein phosphorylation...
  101. pmc Active transcription and essential role of RNA polymerase II at the centromere during mitosis
    F Lyn Chan
    Chromosome and Chromatin Research, Department of Paediatrics, Murdoch Childrens Research Institute, Royal Children s Hospital, University of Melbourne, Parkville, Victoria 3052, Australia
    Proc Natl Acad Sci U S A 109:1979-84. 2012
    ..we investigate whether transcription also occurs and plays a functional role at the mammalian centromere during mitosis. We show the presence of actively transcribing RNA polymerase II (RNAPII) and its associated transcription ..

Research Grants76

  1. Xenoestrogen regulation of Leydig cells
    BENSON TOKUNBO AKINGBEMI; Fiscal Year: 2010
    ..Consistent with this hypothesis are recent observations indicating that genistein regulates Leydig cell mitosis. Also, soy isoflavones are known to regulate development of somatic cells in the testis, e.g. Sertoli cells...
  2. Integrating stress MAP kinase signaling with DNA replication origin licensing
    Jeanette Gowen Cook; Fiscal Year: 2013
    ..that stress MAP kinases also function in unperturbed cell cycles to govern cellular quiescence, G2 phase, and mitosis - normal circumstances in which origin licensing must be inhibited...
  3. PKC delta and cell renewal in irradiated taste epithelium
    MARY ELAINE REYLAND; Fiscal Year: 2012
    ..test the hypothesis that loss of PKC4 protects taste progenitor cells from death, and/or promotes their continued mitosis following irradiation injury...
  4. Functional study of C53 protein as a novel regulator of checkpoint kinases
    Honglin Li; Fiscal Year: 2012
    ..process that includes accurate replication of DNA during the S phase, correct segregation of chromosomes during mitosis (M phase) and final separation of daughter cells in cytokinesis...
  5. ATM Controls Genomic Stability in Pluripotent Stem Cells
    JASON MARK BECKTA; Fiscal Year: 2013
    ..of ATM using the highly-specific kinase inhibitor KU-60019 abrogates this arrest and allows PSCs to enter mitosis, resulting in bizarre chromosome morphology...
  6. Roles of the mammalian CST complex in DNA replication and chromosome cohesion
    JASON AARON STEWART; Fiscal Year: 2013
    ..member and laboratory head at the Memorial Sloan-Kettering Cancer Center and an expert in chromosome cohesion and mitosis. The third aim will use a multi-pronged approach to determine CST interacting partners...
  7. Role of Polo-like kinase (Plk-1) in Hepatitis B Virus-mediated Hepatocellular Car
    Ourania M Andrisani; Fiscal Year: 2010
    ..However, despite DNA damage, pX-expressing hepatocytes do not die and instead proceed to mitosis, where DNA damage is propagated to daughter cells, giving rise to polyploidy...
  8. SIGNALING THROUGH RHO GTP/GDP EXCHANGE FACTORS
    Philip B Wedegaertner; Fiscal Year: 2013
    ..cells, LARG is localized at specific mitotic structures, including centrosomes and mitotic spindles in early mitosis and the cytokinesis cleavage furrow and midbody in late mitosis/cytokinesis...
  9. Systems Analysis of Cardiac Chromatin Structure
    Thomas M Vondriska; Fiscal Year: 2013
    ..expression);yet how the genome is organized in three dimensions, apart from the well-described machinations of mitosis, is only beginning to be understood...
  10. Regulation of TGF-beta Signaling and Embryonic Development by GTPases
    Gerald H Thomsen; Fiscal Year: 2010
    ..provided by applicant): GTPases govern critical cellular processes such as signal transduction, transcription, mitosis, cell shape and cell movement by acting as molecular switches that regulate activity of protein partners...
  11. Role of dyskerin in oral epithelial homeostasis
    Faizan Alawi; Fiscal Year: 2013
    ..However, while both of these cellular processes are repressed during mitosis, we have shown that dyskerin expression peaks during mitosis, the protein localizes to distinct sub-cellular ..
  12. The role of the nucleoporin Nup98 in gene regulation
    Martin W Hetzer; Fiscal Year: 2013
    ..during cell differentiation and development relies on faithful reproduction of a specific chromatin state through mitosis. Failure to maintain these epigenetic programs through multiple cell divisions commonly leads to pathological ..
  13. CELL CYCLE CONTROL IN EARLY DROSOPHILA DEVELOPMENT
    Terry L Orr-Weaver; Fiscal Year: 2013
    ..the modified cell cycle used to produce haploid eggs and sperm, and the activation of DNA replication and mitosis in the zygote that results from fusion of sperm and egg...
  14. Molecular genetics of egg activation and pronuclear function in Drosophila
    MARIANA FEDERICA WOLFNER; Fiscal Year: 2010
    ..For example, activation is needed to block polyspermy, resume meiosis and start embryo mitosis. Although activation is critical for fertility, little is known about its control apart from a key role for ..
  15. Histone Deacetylase Inhibition: A Novel Approach to Cardioprotection
    Ting C Zhao; Fiscal Year: 2012
    ..We also demonstrated that HDAC inhibition resulted in an increase in c-kit stem cells, cell proliferation and mitosis in chronic infarcted hearts...
  16. Centrosomin and Centrosomes in Cell Division
    Timothy L Megraw; Fiscal Year: 2012
    ..in animal cells and are required for efficient assembly of microtubules into the bipolar spindle apparatus at mitosis. The molecular functions of centrosome and centriole proteins are largely uncharacterized;indeed, the centrosome "..
  17. Molecular and cellular mechanism of Microcephaly
    Arnold Kriegstein; Fiscal Year: 2013
    ..Centrosome defects in Drosophila do not dramatically perturb mitosis in most somatic cells, but the asymmetric division of larval neuroblasts is noticeably disrupted, underscoring ..
  18. Novel Role of a Nucleoporin Gene in Atrial Fibrillation, the Most Common Cardiac
    Qing Kenneth Wang; Fiscal Year: 2013
    ..is required for the formation of the nuclear pore complex (NPC) and the assembly of the nuclear envelope during mitosis. The NPC is a large macromolecular complex of about 30 nucleoporins, and plays a key role in bi-directional ..
  19. Establishment of sister chromatid cohesion
    Douglas E Koshland; Fiscal Year: 2013
    ..chromatids (the newly replicated chromosomes) from the time of their synthesis in S phase through metaphase of mitosis. This sister chromatid cohesion is critical to ensure that daughter cells inherit proper ploidy during cell ..
  20. Effect of Major Depression and antidepressants on human neurogenesis
    Maura Boldrini; Fiscal Year: 2013
    ..Immunocytochemistry for Ki67, a cell cycle protein expressed during mitosis, will be used to identify dividing cells...
  21. Plk1-Numb Cooperation in Melanomagenesis
    Nihal Ahmad; Fiscal Year: 2013
    ..study and our preliminary data, we propose to test the hypothesis that Plk1 and Numb work in close concert during mitosis, expression of both are required for proper melanocytic cell division and integrity, and loss of Numb and Plk1 ..
  22. Mechanisms by which VZV IE proteins interact with cell antiviral proteins to prev
    MARIA ACENA NAGEL; Fiscal Year: 2013
    ..Everett and Chelbi-Alix, 2007);in addition, the structure of PML-NBs is dramatically altered in cells undergoing mitosis compared to PML-NB stability in post-mitotic cells such as neurons (Dellaire et al., 2006a)...
  23. Design of a high-throughput screen for chemicals that cause meiotic aneuploidy
    Patrick Allard; Fiscal Year: 2011
    ..from errors in the segregation of chromosomes during the two highly regulated programs of cell division, mitosis and meiosis...
  24. Regulation of chromosome segregation by SMC complexes and Top2 in S. pombe
    MATTHEW J O'CONNELL; Fiscal Year: 2013
    ..that all three SMC complexes and Top2 functionally interact to guide chromosomes through DNA replication and mitosis. While most studies on Smc5/6 have been in the context of DNA repair, this proposal focuses specifically on the ..
  25. Receptor/Ligand Signals for Ovarian Follicular Angiogenesis
    Leon J Spicer; Fiscal Year: 2010
    ..ANG mRNA is increased by insulin-like growth factor-I (IGF1) in granulosa cells;and 3) FGF9 stimulates theca cell mitosis while inhibiting theca cell differentiation...
  26. Novel small-molecule inhibitors of Wee1 kinase for medulloblastoma treatment
    Philip Reigan; Fiscal Year: 2013
    ..The Wee1 kinase participates in the G2-M checkpoint to prevent mitosis in the presence of DNA damage and therefore may play a role in drug resistance to DNA alkylating agents...
  27. Internalization of gap junctions as a regulatory mechanism of direct GJIC
    Matthias M Falk; Fiscal Year: 2013
    ..or need to uncouple for other reasons (mitosis, apoptosis, etc.)...
  28. Replication licensing and cell cycle checkpoints
    Jeanette Gowen Cook; Fiscal Year: 2013
    ..cyclin dependent protein kinases (Cdks) that are activated at the G1/S phase transition and remain active until mitosis. To prevent any sequence from being replicated more than once, origin licensing is inhibited once replication ..
  29. MICROFILAMENTS IN THE YEAST SACCHAROMYCES CEREVISIAE
    Anthony P Bretscher; Fiscal Year: 2013
    ..and the vacuole for segregation during the cell cycle, and microtubule ends for nuclear orientation prior to mitosis. These transport events are all mediated by organelle-specific receptors, many of which are known...
  30. Developmental Regulation of the Cell Cycle in Drosophila
    LAURA ANNE LEE; Fiscal Year: 2013
    ..Because the vertebrate homolog of ASUN also controls dynein localization in cultured human cells during mitosis, elucidation of the mechanism of action of ASUN may have important implications for human diseases such as cancer. ..
  31. Identification of DNA Elements Governing Chromatin Function in C elegans
    Jason D Lieb; Fiscal Year: 2011
    ..activity and other essential processes, including recombination and the faithful segregation of the genome during mitosis and meiosis...
  32. Kinetochore Function and Cell Cycle Progression Revision
    Katsumi Kitagawa; Fiscal Year: 2012
    ..consists of centromere DNA and associated proteins, is crucial for maintaining and segregating chromosomes during mitosis and meiosis...
  33. The Role of HEF1 in Ovarian Cancer Development, Progression, and Metastasis
    Denise C Connolly; Fiscal Year: 2013
    ..protein that mediates cellular signaling from focal adhesions during interphase and at centrosomes during mitosis. Over the past two years, alterations in expression of HEF1 have been shown to play a significant role in cancer ..
  34. Mechanisms of Nuclear and Cell Fusion in Yeast
    MARK DAVID ROSE; Fiscal Year: 2013
    ..Proliferation and differentiation are mutually exclusive;during development cells shut down mitosis as they turn on specialized cell functions...
  35. Fluorescent Copper Probes for Intracellular Imaging
    Christoph J Fahrni; Fiscal Year: 2013
    ..will be applied to elucidate the role of the Golgi apparatus in copper redistribution and inheritance during mitosis and cell proliferation...
  36. Homolog orientation and segregation in acentrosomal meiosis
    Kim S McKim; Fiscal Year: 2013
    ..the microtubule connections formed between the poles and the kinetochores facilitates bi-orientation of sisters (mitosis) or homologous chromosomes (meiosis I)...
  37. Regulation of variable lifespan in castes of ants
    Hua Yan; Fiscal Year: 2013
    ..This effect will be tested immunohistochemically, using the cell mitosis marker phosphor-histone H3 (H3S10P)...
  38. Arsenic Induced Miotic Arrest Associated Apoptosis
    J Christopher States; Fiscal Year: 2009
    ..Arsenite, the carcinogenic form of arsenic found in drinking water, disrupts mitosis causing an anaphase delay and induces aneuploidy in normal diploid human fibroblasts and peripheral blood ..
  39. Role of T-cells in the induction of lupus
    Bruce C Richardson; Fiscal Year: 2013
    ..ERK pathway signaling, resulting in failure to upregulate the maintenance DNA methyltransferase Dnmt1 during mitosis. This results in failure to methylate newly synthesized DNA, altering chromatin structure and causing ..
  40. Characterization of JNK in Cell Cycle Control
    ZE apos EV A RONAI; Fiscal Year: 2012
    ..APC/CCdh1-mediated JNK degradation primarily occurs during the exit from mitosis, and G1 phases of the cell cycle...
  41. Study of Anti-Survival signals in NF1
    CHANGYAN CHEN; Fiscal Year: 2013
    ..If insults target late stages of mitosis, mitotic exit checkpoint would be activated and subsequently induce mitotic catastrophe to eliminate unwanted ..
  42. Activation of Cyclin-Dependent Kinases by Fructose-2,6-Bisphosphate
    JASON A CHESNEY; Fiscal Year: 2013
    ..to regulatory cyclins and phosphorylate hundreds of substrates that control DNA replication, transcription and mitosis. We found that over-expression of PFKFB3 stimulated CDK1 activity in HeLa cells and that purified F2,6BP ..
  43. Condensin II/RBF
    Nicholas J Dyson; Fiscal Year: 2013
    ..Condensin complexes are conserved from yeast to humans and are required for normal chromosome structure during mitosis. Experiments using the Drosophila model system show that dCAP-D3 is extremely important for RBF1 function...
  44. STUDIES ON OVARIAN RING CANALS IN DROSOPHILA
    Lynn Cooley; Fiscal Year: 2009
    ..Thus, ring canal development begins as a modified mitosis. They are best characterized so far in Drosophila females where ring canals allow the flow of cytoplasm from ..
  45. Cellular Biomechanics of Heat-Shock Induced Defects in Drosophila Embryogenesis
    M Shane Hutson; Fiscal Year: 2013
    ..The four specific aims of this project are: 1. To evaluate the correlation between an extra 14th mitosis in the amnioserosa - a tissue which plays a critical mechanical role in embryogenesis - and subsequent defects in ..
  46. Regulation of Borealin Function by Mitotic Phosphorylation
    William Taylor; Fiscal Year: 2009
    Eukaryotic cells divide by a complicated process involving multiple, highly coordinated events. Mitosis, during which duplicated chromosomes are segregated to opposite poles of the cell must occur accurately to ensure that daughter ..
  47. Taste and Cyclophosphamide in Mice
    Eugene R Delay; Fiscal Year: 2013
    ..However, no one has asked how these drugs affect taste epithilium. CYP damages DNA during mitosis and thus targets cells engaged in high rates of mitosis such as cancer or adult progenitor cells of tissues with ..
  48. Impact of hepatocyte lineage life history dynamics on liver homeostasis in the ag
    Edward E Schmidt; Fiscal Year: 2013
    ..Hepatocytes are also one of few cell types that undergo endoreplication and acytokinetic mitosis, resulting in polyploid nuclei and bi-nucleate cells, respectively...
  49. The Role of Cytoplasmic Dynein in Axonal Transport
    KEVIN K PFISTER; Fiscal Year: 2011
    ..In the third aim we will investigate the functional significance of DYNLT3 and this binding to mitosis, and identify the distinct domains of DYNLT3 that are responsible for the specific binding...
  50. REGULATION OF THE DEVELOPMENT OF TRYPANOSOMA BRUCEI
    CHING CHUNG WANG; Fiscal Year: 2012
    ..brucei. We also observed that a block of mitosis achieves genuine mitotic arrest in the procylic form but not in the bloodstream form, which continues with re-..
  51. The RAN GTPase
    Ian G Macara; Fiscal Year: 2013
    ..of its catalytic action, and that phosphorylation of the N-terminal tail stabilizes chromatin association during mitosis. We will rigorously test this model, by replacing endogenous RCC1 with tethered fusion proteins and ..
  52. PTEN Deficiency and Tumor Development
    Yuxin Yin; Fiscal Year: 2012
    ..To do so, we will identify potential kinetochore or spindle factors physically associated with PTEN during mitosis. We will also determine whether wild-type PTEN can correct mitotic errors and restore the mitotic checkpoint...
  53. Molecular Mechanisms of Cytoskeletal Regulators
    Kevin C Slep; Fiscal Year: 2013
    ..a dynamic, polarized cellular scaffold that facilitates intracellular transport, ciliogenesis, cell migration and mitosis;all processes that rely on the regulated dynamic instability of the microtubule polymer...
  54. The Nuclear Pore Complex: Interphase and Mitotic Function
    Katharine S Ullman; Fiscal Year: 2012
    ..The long-term goal of this project is to elucidate how events of mitosis are orchestrated...
  55. ROCK II-mediated regulation of centrosome duplication / centrosome amplification
    Kenji Fukasawa; Fiscal Year: 2013
    ..The detrimental consequence of centrosome amplification is prominently featured during mitosis: the presence of multiple centrosomes (spindle poles) results in mitotic defects, leading to an increased ..
  56. Identification and characterization of novel epigenetic marks of non-histone prot
    Xiaodong Cheng; Fiscal Year: 2010
    ..residues in particular has been shown to function in a number of cellular processes, including transcription, mitosis, apoptosis, and sporulation...
  57. Studying Mitosis using Xenopus Egg Extracts
    Rebecca W Heald; Fiscal Year: 2013
    ..The general goal of the project is to elucidate the principles that underlie the dynamic events of mitosis, as well as to identify and study the roles of individual proteins...
  58. Vitamin A dependent events in urinary tract formation.
    Cathy Mendelsohn; Fiscal Year: 2009
    ..and a novel domain of Raldh2 expression at the cortico-medullary junction where local proliferation and oriented mitosis occur during papilla formation, suggesting that RA may be important for these processes...
  59. The Role of DRP-1 and Mitochondrial Fission in Pulmonary Arterial Hypertension
    Stephen L Archer; Fiscal Year: 2013
    ..of DRP-1 at Serine 616 by the key mitotic regulatory kinase, Cyclin B1- CDK1, thus linking fission and mitosis. Elevated cytosolic calcium in PAH also promotes DRP-1-mediated fission by: a) activation of calmodulin kinase ..
  60. Molecular mechanisms underlying chromosomal instability and aneuploidy
    Wei Dai; Fiscal Year: 2012
    ..Aneuploidy, a common form of genomic instability, is a direct result of chromosomal missegregation during mitosis. Over the course of evolution, eukaryotic cells have developed sophisticated molecular mechanisms to maintain the ..
  61. Regulation of the cell cycle by SCF and APC/C ubiquitin ligases
    Michele Pagano; Fiscal Year: 2013
    ..We also demonstrated that [unreadable]TrCP, another F-box protein, allows precise regulation of the critical mitosis regulator Cdk1 by targeting Cdc25A, Claspin, Rest, and Emi1 for degradation...
  62. Stochastic Models of Cell Cycle Regulation in Eukaryotes
    John J Tyson; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The cycle of cell growth, DNA synthesis, mitosis and cell division is the fundamental process by which cells (and all living organisms) grow, develop and reproduce...
  63. PAIRING OF HOMOLOGOUS SEQUENCES IN DROSOPHILA
    KENT GOLIC; Fiscal Year: 2002
    ..lab has recently obtained a body of evidence supporting the idea that homologous chromosome pairing during mitosis serves as a close model for meiotic chromosome pairing. Most convincingly, he has in collaboration with Dr...
  64. FORMATION,INTERACTION & FUNCTION OF SPINDLE COMPONENTS
    Conly L Rieder; Fiscal Year: 2010
    The broad objectives of this proposal are to determine how entry and exit from mitosis is controlled, how centrosomes and kinetochores function and interact to form the spindle, and how the forces for chromosome motion &positioning ..
  65. MOLECULAR ANALYSIS OF CHROMOSOME SEGREGATION IN YEAST
    Clarence Chan; Fiscal Year: 1999
    DESCRIPTION: This proposal is directed at understanding the requirements for proper chromosome segregation during mitosis. Using a clever screen to detect mutations which lead to increases in ploidy, the PI previously isolated a ..
  66. Function of the chromosomal kinase haspin in mitosis
    JONATHAN M HIGGINS; Fiscal Year: 2010
    ..associates with condensed chromosomes, particularly at centromeres, and is also found at the centrosomes during mitosis. Importantly, haspin RNAi causes misalignment of metaphase chromosomes and spindle defects, and overexpression ..
  67. THE ROLE OF PIN1 IN MITOSIS WITH DESIGNED INHIBITORS
    FELICIA ETZKORN; Fiscal Year: 2002
    DESCRIPTION: Pin 1, a newly discovered regulator of mitosis signal transduction, is an enzyme that acts on phosphoserine-proline amides...
  68. S POMBE PROTEIN/CENTROMERIC DNA INTERACTION
    Jerard Hurwitz; Fiscal Year: 2001
    ..The area that has only recently begun to be attacked at the biochemical level concerns mitosis, the important biological process by which chromosomes are sorted, paired and accurately distributed to each ..
  69. Using Chemical Biology to Study the Small GTPase Ra(RMI)
    Karsten Weis; Fiscal Year: 2005
    ..transport during interphase and recently it has been demonstrated that Ran also regulates key event in mitosis such as spindle assembly, nuclear envelope assembly and nuclear pore complex assembly...
  70. REGULATION OF THE DEVELOPMENT OF TRYPANOSOMA BRUCEI
    Ching Wang; Fiscal Year: 2009
    ..brucci. We also observed that a block of mitosis achieves genuine mitotic T. brucei...
  71. REGULATION OF MITOSIS IN NORMAL AND TRANSFORMED CELLS
    Robert Schlegel; Fiscal Year: 1993
    ..An important contributor to such cytogenetic damage is thought to be the loss of normal regulation of mitosis. The aim of this research project is to gain an understanding of the molecular events that control mitotic onset, ..
  72. BIMA AND MITOSIS
    PETER MIRABITO; Fiscal Year: 1999
    The metaphase to anaphase transition is a complex and critical step in mitosis. Multiple regulatory signals impinge on the decision to initiate sister chromatid separation, including those involved in nonessential checkpoint regulation ..
  73. G2 CHECKPOINT FUNCTION IN HUMAN FIBROBLASTS
    William Kaufmann; Fiscal Year: 2007
    ..During mitosis, the replicated chromatin fibers are condensed and compacted into sister chromatids and then the sister ..
  74. WHY IS HISTONE ACETYLATION REDUCED AT MITOSIS?
    James Paulson; Fiscal Year: 1992
    ..reversible acetylation at certain lysine residues, but this acetylation is drastically reduced during normal mitosis and in metaphase-arrested cells...
  75. A NOVEL F BOX PROTEIN REGULATING MITOSIS
    Peter Jackson; Fiscal Year: 2004
    ..from the applicant's abstract): The accumulation of cyclin B and activation of the cyclin B/Cdc2 kinase drives mitosis. The ubiquitination of cyclin B by a mitotically activated ubiquitin ligase, the Anaphase Promoting Complex (APC),..
  76. PPK: Radiation Response and Cell Proliferation
    Peter Stambrook; Fiscal Year: 2006
    ..They participate in the transit of cells from late G2 into mitosis, through mitosis and exit from mitosis...