metaphase

Summary

Summary: The phase of cell nucleus division following PROMETAPHASE, in which the CHROMOSOMES line up across the equatorial plane of the MITOTIC SPINDLE APPARATUS prior to separation.

Top Publications

  1. pmc Uncoordinated loss of chromatid cohesion is a common outcome of extended metaphase arrest
    Deanna Stevens
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 6:e22969. 2011
  2. ncbi An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast
    R Ciosk
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 93:1067-76. 1998
  3. pmc OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis
    Tian Shao
    State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    Plant Physiol 156:1386-96. 2011
  4. pmc Mechanisms of microtubule-based kinetochore positioning in the yeast metaphase spindle
    Brian L Sprague
    Department of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Biophys J 84:3529-46. 2003
  5. ncbi Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles. Implications for the role of microtubule dynamics in mitosis
    M A Jordan
    Department of Biological Sciences, University of California, Santa Barbara 93106
    J Cell Sci 102:401-16. 1992
  6. ncbi Dual inhibition of sister chromatid separation at metaphase
    O Stemmann
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 107:715-26. 2001
  7. pmc Spindle pole bodies exploit the mitotic exit network in metaphase to drive their age-dependent segregation
    Manuel Hotz
    Institute of Biochemistry, Biology Department, ETH Zurich, 8093 Zurich, Switzerland
    Cell 148:958-72. 2012
  8. pmc Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport
    J Pines
    Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, San Diego, California 92186 5800
    J Cell Biol 115:1-17. 1991
  9. pmc Aurora B regulates formin mDia3 in achieving metaphase chromosome alignment
    Lina Cheng
    Department of Pathology and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Dev Cell 20:342-52. 2011
  10. ncbi Activation of p53 or loss of the Cockayne syndrome group B repair protein causes metaphase fragility of human U1, U2, and 5S genes
    A Yu
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Mol Cell 5:801-10. 2000

Research Grants

  1. Functional Analysis of the Dual Specificity Kinase NEK1 in Mammalian Meiosis
    JOANNA KIM HOLLOWAY; Fiscal Year: 2011
  2. Cell Cycle Regulation and Adult T Cell Leukemia
    Chou Zen Giam; Fiscal Year: 2013
  3. RADIATION-INDUCED THYROID CANCER
    Heinz Ulrich G Weier; Fiscal Year: 2011
  4. Molecular Pathogenesis of MDS and CMML
    Jaroslaw P Maciejewski; Fiscal Year: 2013
  5. Regulation of EBV Latency by EBNA1
    Paul M Lieberman; Fiscal Year: 2013
  6. ATM Controls Genomic Stability in Pluripotent Stem Cells
    JASON MARK BECKTA; Fiscal Year: 2013
  7. Functional Analysis of the Dual Specificity Kinase NEK1 in Mammalian Meiosis
    JOANNA KIM HOLLOWAY; Fiscal Year: 2013
  8. ANALYSIS OF MEIOTIC CHROMOSOME SYNAPSIS IN YEAST
    Nancy M Hollingsworth; Fiscal Year: 2013
  9. The Role of Cks Proteins in Mammalian Meiosis
    Charles H Spruck; Fiscal Year: 2010
  10. HTLV-I Tax activates the anaphase promoting complex
    Chou Zen Giam; Fiscal Year: 2013

Detail Information

Publications307 found, 100 shown here

  1. pmc Uncoordinated loss of chromatid cohesion is a common outcome of extended metaphase arrest
    Deanna Stevens
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 6:e22969. 2011
    ..Chromatid separation at the metaphase-to-anaphase transition is accomplished by cleavage of the cohesin complex that holds chromatids together...
  2. ncbi An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast
    R Ciosk
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 93:1067-76. 1998
    ..Loss of cohesion at the metaphase to anaphase transition is accompanied by Scc1p's dissociation from chromatids, which depends on proteolysis of ..
  3. pmc OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis
    Tian Shao
    State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    Plant Physiol 156:1386-96. 2011
    ..that OsREC8 encodes a protein that localized to meiotic chromosomes from approximately meiotic interphase to metaphase I. Homologous pairing and telomere bouquet formation were abnormal in Osrec8 meiocytes...
  4. pmc Mechanisms of microtubule-based kinetochore positioning in the yeast metaphase spindle
    Brian L Sprague
    Department of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Biophys J 84:3529-46. 2003
    ..To visualize metaphase kMT plus-end dynamics in yeast, a strain containing a green fluorescent protein fusion to the kinetochore ..
  5. ncbi Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles. Implications for the role of microtubule dynamics in mitosis
    M A Jordan
    Department of Biological Sciences, University of California, Santa Barbara 93106
    J Cell Sci 102:401-16. 1992
    ..drugs induced a nearly identical rearrangement of spindle microtubules, an increasingly aberrant organization of metaphase chromosomes, and fragmentation of centrosomes...
  6. ncbi Dual inhibition of sister chromatid separation at metaphase
    O Stemmann
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 107:715-26. 2001
    ..Separase is inhibited by securin, which is degraded at the metaphase-anaphase transition...
  7. pmc Spindle pole bodies exploit the mitotic exit network in metaphase to drive their age-dependent segregation
    Manuel Hotz
    Institute of Biochemistry, Biology Department, ETH Zurich, 8093 Zurich, Switzerland
    Cell 148:958-72. 2012
    ..During metaphase, the spindle positioning protein Kar9 accumulates asymmetrically, localizing specifically to astral microtubules ..
  8. pmc Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport
    J Pines
    Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, San Diego, California 92186 5800
    J Cell Biol 115:1-17. 1991
    ..A may associated with condensing chromosomes in prophase, but is not associated with condensed chromosomes in metaphase. By contrast, cyclin B1 accumulates in the cytoplasm of interphase cells and only enters the nucleus at the ..
  9. pmc Aurora B regulates formin mDia3 in achieving metaphase chromosome alignment
    Lina Cheng
    Department of Pathology and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Dev Cell 20:342-52. 2011
    ..Here we show an essential role of the formin mDia3 in achieving metaphase chromosome alignment...
  10. ncbi Activation of p53 or loss of the Cockayne syndrome group B repair protein causes metaphase fragility of human U1, U2, and 5S genes
    A Yu
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Mol Cell 5:801-10. 2000
    Infection by adenovirus 12, transfection with the Ad12 E1B 55 kDa gene, or activation of p53 cause metaphase fragility of four loci (RNU1, PSU1, RNU2, and RN5S) each containing tandemly repeated genes for an abundant small RNA (U1, U2, ..
  11. pmc The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells
    Ryota Uehara
    Institute for Advanced Research, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    Proc Natl Acad Sci U S A 106:6998-7003. 2009
    ..in HeLa cells triggers the spindle checkpoint, reduces tension on sister kinetochores, and severely impairs metaphase progression...
  12. pmc Molecular control of kinetochore-microtubule dynamics and chromosome oscillations
    Ana C Amaro
    Institute of Biochemistry, Eidgenossische Technische Hochschule ETH Zurich, CH 8093 Zurich, Switzerland
    Nat Cell Biol 12:319-29. 2010
    Chromosome segregation in metazoans requires the alignment of sister kinetochores on the metaphase plate...
  13. ncbi Stable kinetochore-microtubule attachment constrains centromere positioning in metaphase
    Chad G Pearson
    Department of Biology, University of North Carolina at Chapel Hill, Coker Hall Call Box 3280, Chapel Hill, NC 27599, USA
    Curr Biol 14:1962-7. 2004
    ..distinctly separated from their sisters, and positioned equidistant from their respective spindle poles during metaphase. However, individual fluorescent chromosome markers near the centromere transiently reassociate with their ..
  14. pmc Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin
    S Tada
    CRC Chromosome Replication Research Group, Wellcome Trust Biocentre, Dow Street University of Dundee, Dundee DD1 5EH, UK
    Nat Cell Biol 3:107-13. 2001
    ..Immunodepletion of geminin from metaphase extracts allowed them to assemble licensed replication origins...
  15. ncbi A simple method for simultaneous interphase-metaphase chromosome analysis in biodosimetry
    M Durante
    Space and Particle Radiation Group, National Institute of Radiological Sciences, Chiba, Japan
    Int J Radiat Biol 74:457-62. 1998
    ..To find a simple protocol for measuring chromosome damage both in G1 and in G2/M chromosomes, to overcome problems related to low mitotic index and cell-cycle alterations in biodosimetric tests...
  16. pmc Induction of robust de novo centrosome amplification, high-grade spindle multipolarity and metaphase catastrophe: a novel chemotherapeutic approach
    V Pannu
    Department of Biology, Georgia State University, Atlanta, GA, USA
    Cell Death Dis 3:e346. 2012
    ..aberrant cytokinesis following an 'anaphase catastrophe', most mitotically arrested cells (∼70%) succumbed to 'metaphase catastrophe,' which was caspase-independent...
  17. ncbi Cohesin SMC1 beta is required for meiotic chromosome dynamics, sister chromatid cohesion and DNA recombination
    Ekaterina Revenkova
    Center for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    Nat Cell Biol 6:555-62. 2004
    ..Male meiosis is blocked in pachytene; female meiosis is highly error-prone but continues until metaphase II...
  18. pmc Misorientation and reduced stretching of aligned sister kinetochores promote chromosome missegregation in EB1- or APC-depleted cells
    V M Draviam
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    EMBO J 25:2814-27. 2006
    ..binding partner, EB1, are unusual in affecting the movement and orientation of paired sister chromatids at the metaphase plate without perturbing kinetochore-MT attachment per se...
  19. doi Nucleation and transport organize microtubules in metaphase spindles
    Jan Brugués
    Department of Molecular and Cellular Biology and Center for Systems Biology, Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA
    Cell 149:554-64. 2012
    ..We used this method to study the metaphase spindle in Xenopus laevis egg extracts and found that microtubules are shortest near poles and become ..
  20. pmc The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment
    Jun Dai
    Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 19:472-88. 2005
    ..Haspin RNA interference causes misalignment of metaphase chromosomes, and overexpression delays progression through early mitosis...
  21. ncbi Kinetochore microtubule dynamics and attachment stability are regulated by Hec1
    Jennifer G DeLuca
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Cell 127:969-82. 2006
    ..These findings reveal a key role for the Hec1 N terminus in controlling dynamic behavior of kinetochore microtubules...
  22. pmc Mitotic CDKs control the metaphase-anaphase transition and trigger spindle elongation
    Rami Rahal
    Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 USA
    Genes Dev 22:1534-48. 2008
    ..for the activation of the ubiquitin ligase Anaphase-Promoting Complex/Cyclosome (APC/C)-Cdc20 that triggers the metaphase-anaphase transition...
  23. pmc Pericentric chromatin is an elastic component of the mitotic spindle
    David C Bouck
    Department of Biology, University of North Carolina at Chapel Hill, North Carolina 27599, USA
    Curr Biol 17:741-8. 2007
    Prior to chromosome segregation, the mitotic spindle bi-orients and aligns sister chromatids along the metaphase plate...
  24. pmc Lis1/dynactin regulates metaphase spindle orientation in Drosophila neuroblasts
    Karsten H Siller
    Howard Hughes Medical Institute, Institutes of Molecular Biology and Neuroscience, University of Oregon, Eugene, OR 97403, USA
    Dev Biol 319:1-9. 2008
    ..Loss of either astral microtubules or Lis1/dynactin leads to spindle/cortical polarity alignment defects at metaphase, but these are rescued by telophase...
  25. pmc Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases
    Khuloud Jaqaman
    Marine Biological Laboratory, Woods Hole, MA 02543, USA
    J Cell Biol 188:665-79. 2010
    During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement...
  26. pmc The Schizosaccharomyces pombe spindle checkpoint protein mad2p blocks anaphase and genetically interacts with the anaphase-promoting complex
    X He
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 94:7965-70. 1997
    ..fission yeast, and shown that mad2 overexpression activates the checkpoint and causes a cell cycle arrest at the metaphase-to-anaphase transition...
  27. ncbi Microtubule-induced Pins/Galphai cortical polarity in Drosophila neuroblasts
    Sarah E Siegrist
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon 97403, USA
    Cell 123:1323-35. 2005
    ..neuroblast cortical polarity with CNS tissue polarity, whereas the microtubule/Khc-73/Dlg pathway functions at metaphase to coordinate neuroblast cortical polarity with the mitotic spindle axis...
  28. pmc Force and length in the mitotic spindle
    Sophie Dumont
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    Curr Biol 19:R749-61. 2009
    The mitotic spindle assembles to a steady-state length at metaphase through the integrated action of molecular mechanisms that generate and respond to mechanical forces...
  29. ncbi Inhibition of DNA decatenation, but not DNA damage, arrests cells at metaphase
    Dimitrios A Skoufias
    Institut de Biologie Structurale J P Ebel CEA CNRS, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France
    Mol Cell 15:977-90. 2004
    ..Durable arrest at metaphase occurs, however, with ICRF-193, a topoisomerase II inhibitor that does not damage DNA...
  30. pmc REEP3/4 ensure endoplasmic reticulum clearance from metaphase chromatin and proper nuclear envelope architecture
    Anne Lore Schlaitz
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Dev Cell 26:315-23. 2013
    ..This phenotype originates in mitosis, when ER membranes accumulate on metaphase chromosomes...
  31. pmc Mouse Emi2 as a distinctive regulatory hub in second meiotic metaphase
    Toru Suzuki
    Laboratory of Mammalian Molecular Embryology, Bath Centre for Regenerative Medicine, and Development of Biology and Biochemistry, University of Bath, Bath, UK
    Development 137:3281-91. 2010
    The oocytes of vertebrates are typically arrested at metaphase II (mII) by the cytostatic factor Emi2 until fertilization...
  32. pmc Insights into the micromechanical properties of the metaphase spindle
    Yuta Shimamoto
    Laboratory of Chemistry and Cell Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Cell 145:1062-74. 2011
    The microtubule-based metaphase spindle is subjected to forces that act in diverse orientations and over a wide range of timescales...
  33. doi A model for chromosome condensation based on the interplay between condensin and topoisomerase II
    Jonathan Baxter
    Genome Damage and Stability Centre, Science Park Road, University of Sussex, Falmer, Brighton, East Sussex, BN1 9RQ, UK
    Trends Genet 28:110-7. 2012
    ..We propose a model of how metaphase chromosomes could be shaped based on the enzymatic activities of condensin and topoisomerase II in overwinding ..
  34. doi Constant regulation of both the MPF amplification loop and the Greatwall-PP2A pathway is required for metaphase II arrest and correct entry into the first embryonic cell cycle
    Thierry Lorca
    Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoleculaire, CNRS UMR 5237, IFR 122, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    J Cell Sci 123:2281-91. 2010
    ..Our results show that removal of Cdc25 from metaphase-II-arrested oocytes promotes mitotic exit, whereas depletion of either Myt1 or Wee1 in interphase egg extracts ..
  35. pmc The spindle checkpoint protein Mad2 regulates APC/C activity during prometaphase and metaphase of meiosis I in Saccharomyces cerevisiae
    Dai Tsuchiya
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    Mol Biol Cell 22:2848-61. 2011
    ..both meiotic divisions, but securin, a substrate of the APC/C, is degraded prematurely, and prometaphase I/metaphase I is accelerated...
  36. ncbi Mitochondrial aggregation patterns and activity in human oocytes and preimplantation embryos
    M Wilding
    Centre for Reproductive Biology, Clinica Villa del Sole, and Dipartimento Clinica di Emergenza Ginecologica e Ostetrica e Medicina della Riproduzione, Azienda Universitaria Policlinico, , Naples, Italy
    Hum Reprod 16:909-17. 2001
    ..The activity of mitochondria in fresh metaphase II oocytes was negatively correlated with maternal age...
  37. doi A deadenylation negative feedback mechanism governs meiotic metaphase arrest
    Eulàlia Belloc
    Centre for Genomic Regulation CRG, Pompeu Fabra University UPF, C Dr Aiguader 88, 08003, Barcelona, Spain
    Nature 452:1017-21. 2008
    ..This negative feedback loop is required to exit from metaphase into interkinesis and for meiotic progression.
  38. pmc Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis
    Fabian Oceguera-Yanez
    Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606 8501, Japan
    J Cell Biol 168:221-32. 2005
    ..We recently suggested that Cdc42 works in metaphase by regulating bi-orient attachment of spindle microtubules to kinetochores...
  39. pmc Human chromokinesin KIF4A functions in chromosome condensation and segregation
    Manjari Mazumdar
    National Cancer Institute, National Institutes of Health, Bldg 41, Rm B 507, 41 Library Dr, Bethesda, MD 20892, USA
    J Cell Biol 166:613-20. 2004
    Accurate chromosome alignment at metaphase and subsequent segregation of condensed chromosomes is a complex process involving elaborate and only partially characterized molecular machinery...
  40. doi Ultrastructural evaluation of human metaphase II oocytes after vitrification: closed versus open devices
    Antonella Bonetti
    Department of Medical Morphological Research, Medical School, University of Udine, Udine, Italy
    Fertil Steril 95:928-35. 2011
    ..To compare the ultrastructural appearance of oocytes after vitrification and warming with two different devices...
  41. doi The small GTPase Cdc42 promotes membrane protrusion during polar body emission via ARP2-nucleated actin polymerization
    J Leblanc
    Ottawa Hospital Research Institute, Ottawa Hospital Civic Campus, Canada
    Mol Hum Reprod 17:305-16. 2011
    ..We report here that treating oocytes with low concentrations of nocodazole diminished the size of metaphase I spindles and prevented polar body emission, and yet an active Cdc42 cap of correspondingly diminished size ..
  42. pmc Aurora-A phosphorylates Augmin complex component Hice1 protein at an N-terminal serine/threonine cluster to modulate its microtubule binding activity during spindle assembly
    Connie Y Tsai
    Department of Biological Chemistry, University of California, Irvine, California 92697 4037, USA
    J Biol Chem 286:30097-106. 2011
    ..antibody revealed that phosphorylated Hice1 primarily localizes to spindle poles during prophase to metaphase but gradually diminishes after anaphase...
  43. pmc Identification of novel microRNAs in Xenopus laevis metaphase II arrested eggs
    Sakthikumar Ambady
    Department of Biomedical Engineering, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA
    Genesis 50:286-99. 2012
    ..and bioinformatics approach, we for the first time identify miRNAs and their relative abundance in mature, metaphase II arrested eggs in Xenopus laevis...
  44. pmc The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells
    Xiaohu Wan
    Biology Department, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Biol Cell 23:1035-46. 2012
    ..the positions of fluorescent kinetochores and their poles for oscillating chromosomes within spindles of metaphase PtK1 cells. We found that the kinetics of P and AP movement are nonlinear and different...
  45. doi Slow freezing and vitrification differentially modify the gene expression profile of human metaphase II oocytes
    C Monzo
    CHU Montpellier, Institut de Recherche en Biothérapie, Hopital Saint Eloi, Montpellier F 34295, France
    Hum Reprod 27:2160-8. 2012
    ..The aim of this study was to examine the effect of the two cryopreservation procedures, slow freezing and vitrification, on the gene expression profile of human metaphase II (MII) oocytes.
  46. ncbi Nuclear dispositions of subtelomeric and pericentromeric chromosomal domains during meiosis in asynaptic mutants of rye (Secale cereale L.)
    E I Mikhailova
    Dept of Genetics, Saint-Petersburg State University, Russia
    J Cell Sci 114:1875-82. 2001
    ..Consequently, recombination is severely impaired, and high univalency is observed at metaphase I...
  47. pmc Role for G-quadruplex RNA binding by Epstein-Barr virus nuclear antigen 1 in DNA replication and metaphase chromosome attachment
    Julie Norseen
    The Wistar Institute, Philadelphia, PA 19104, USA
    J Virol 83:10336-46. 2009
    ..BRACO-19 treatment also disrupted the ability of EBNA1 to tether to metaphase chromosomes, suggesting that maintenance function is also mediated through G-quadruplex recognition...
  48. pmc Functional interaction between p90Rsk2 and Emi1 contributes to the metaphase arrest of mouse oocytes
    Maria Paola Paronetto
    Department of Public Health and Cell Biology, Section of Anatomy, University of Rome Tor Vergata, Rome, Italy
    EMBO J 23:4649-59. 2004
    Vertebrate eggs arrest at metaphase of the second meiotic division before fertilization under the effect of a cytostatic factor (CSF)...
  49. pmc The chromokinesin, KLP3A, dives mitotic spindle pole separation during prometaphase and anaphase and facilitates chromatid motility
    Mijung Kwon
    University of California Davis, Davis, California 95616, USA
    Mol Biol Cell 15:219-33. 2004
    ..Kinetic analysis suggests that KLP3A contributes to spindle pole separation during the prometaphase-to-metaphase transition (when it antagonizes Ncd) and anaphase B, to normal rates of chromatid motility during anaphase A, ..
  50. doi High-resolution mapping of interstitial telomeric repeats in Syrian hamster metaphase chromosomes
    S Demin
    Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia
    Cytogenet Genome Res 132:151-5. 2011
    Karyotype analysis of the Syrian hamster (Mesocricetus auratus) was performed after DAPI-banding of metaphase chromosomes obtained from cultivated skin fibroblasts of a newborn animal...
  51. doi Atomic force microscopy for imaging human metaphase chromosomes
    Tatsuo Ushiki
    Division of Microscopic Anatomy and Bio imaging, Niigata University Graduate School of Medical and Dental Sciences, 1 757 Asahimachi dori, Chuo Ku, Niigata, 951 8510, Japan
    Chromosome Res 16:383-96. 2008
    The present study introduces the principle of atomic force microscopy (AFM) and reviews our results of human metaphase chromosomes obtained by AFM...
  52. pmc Tension-dependent regulation of microtubule dynamics at kinetochores can explain metaphase congression in yeast
    Melissa K Gardner
    Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Biol Cell 16:3764-75. 2005
    During metaphase in budding yeast mitosis, sister kinetochores are tethered to opposite poles and separated, stretching their intervening chromatin, by singly attached kinetochore microtubules (kMTs)...
  53. pmc Lack of response to unaligned chromosomes in mammalian female gametes
    Jaroslava Sebestova
    Institute of Animal Physiology and Genetics, Libechov, Czech Republic
    Cell Cycle 11:3011-8. 2012
    ..crosses, demonstrate that multiple unaligned kinetochores and severe congression defects are tolerated at the metaphase to anaphase transition, although such cells retain sensitivity to nocodazole...
  54. ncbi Inner mitochondrial membrane potential (DeltaPsim), cytoplasmic ATP content and free Ca2+ levels in metaphase II mouse oocytes
    Jonathan Van Blerkom
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Hum Reprod 18:2429-40. 2003
    ..This study examined the response of cultured somatic cells and mouse oocytes to inhibitors and conditions that affect DeltaPsim or metabolism, or both, and measured treatment-specific changes in ATP and cytoplasmic free Ca(2+)...
  55. pmc Gamma-tubulin is required for bipolar spindle assembly and for proper kinetochore microtubule attachments during prometaphase I in Drosophila oocytes
    Stacie E Hughes
    Stowers Institute for Medical Research, Kansas City, Missouri, United States of America
    PLoS Genet 7:e1002209. 2011
    ..Although spindle bipolarity was sometimes achieved by metaphase I in both γtub37C mutants, the resulting chromosome masses displayed highly disrupted chromosome alignment...
  56. ncbi Depletion of topoisomerase IIalpha leads to shortening of the metaphase interkinetochore distance and abnormal persistence of PICH-coated anaphase threads
    Jennifer M Spence
    Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
    J Cell Sci 120:3952-64. 2007
    ..gene encoding DNA topoisomerase IIalpha, we find that depletion of topo IIalpha, while leading to a disorganised metaphase plate, does not have any overt effect on general assembly of kinetochores...
  57. ncbi CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment
    K W Wood
    Laboratory of Cell Biology, Ludwig Institute for Cancer Research, University of California at San Diego, La Jolla 92093 0660, USA
    Cell 91:357-66. 1997
    ..exerted at kinetochores, in combination with polar ejection forces, drive congression of chromosomes to the metaphase plate...
  58. ncbi Unified mode of centromeric protection by shugoshin in mammalian oocytes and somatic cells
    Jibak Lee
    Laboratory of Reproductive Biology and Biotechnology, Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
    Nat Cell Biol 10:42-52. 2008
    ..in prophase or prometaphase, but their localizations become separate when centromeres are pulled oppositely at metaphase. Remarkably, if tension is artificially removed from the centromeres at the metaphase-anaphase transition, ..
  59. ncbi The mammalian passenger protein TD-60 is an RCC1 family member with an essential role in prometaphase to metaphase progression
    Cristiana Mollinari
    Institut de Biologie Structurale J P Ebel, CEA CNRS, 41 rue Jules Horowitz, 38027 Cedex 1, Grenoble, France
    Dev Cell 5:295-307. 2003
    ..The latter is consistent with a TD-60 requirement for recruitment of the passenger proteins survivin and Aurora B, and suggests that like other passenger proteins, TD-60 is involved in regulation of cell cleavage...
  60. ncbi Chfr defines a mitotic stress checkpoint that delays entry into metaphase
    D M Scolnick
    The Wistar Institute, Philadelphia, Pennsylvania 19104 4268, USA
    Nature 406:430-5. 2000
    ..processes include separation of the centrosomes in prophase, alignment of the chromosomes on the spindle in metaphase and sister-chromatid separation in anaphase. Many human cancers are sensitive to mitotic stress...
  61. pmc The polarity and dynamics of microtubule assembly in the budding yeast Saccharomyces cerevisiae
    P S Maddox
    Department of Biology, CB3280, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Nat Cell Biol 2:36-41. 2000
    ..After laser-photobleaching, metaphase spindles recover about 63% of the bleached fluorescence, with a half-life of about 1 minute...
  62. pmc Inhibition of centromere dynamics by eribulin (E7389) during mitotic metaphase
    Tatiana Okouneva
    Department of Molecular, Cellular, and Developmental Biology and Neuroscience Research Institute, University of California Santa Barbara, Santa Barbara, CA 93106, USA
    Mol Cancer Ther 7:2003-11. 2008
    ..The strong correlation between suppression of kinetochore-microtubule dynamics and mitotic arrest indicates that the primary mechanism by which eribulin blocks mitosis is suppression of spindle microtubule dynamics...
  63. ncbi Maximal chromosome compaction occurs by axial shortening in anaphase and depends on Aurora kinase
    Felipe Mora-Bermúdez
    Gene Expression and Cell Biology Unit, European Molecular Biology Laboratory, Meyerhofstr 1, 69117 Heidelberg, Germany
    Nat Cell Biol 9:822-31. 2007
    ..Surprisingly, maximal compaction was not reached in metaphase, but in late anaphase, after sister chromatid segregation...
  64. ncbi A conditional lethal mutant in the fission yeast 26 S protease subunit mts3+ is defective in metaphase to anaphase transition
    C Gordon
    Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh, Scotland, United Kingdom
    J Biol Chem 271:5704-11. 1996
    ..permissive temperature is resistant to the mitotic poison MBC and at the restrictive temperature is defective in metaphase to anaphase transition...
  65. pmc Embryo development of fresh 'versus' vitrified metaphase II oocytes after ICSI: a prospective randomized sibling-oocyte study
    Laura Rienzi
    G E N E R A Centre for Reproductive Medicine, Clinica Valle Giulia, Via De Notaris 2b, Rome, Italy
    Hum Reprod 25:66-73. 2010
    ..Vitrification has been recently proposed as an effective procedure for this purpose...
  66. ncbi Polo-like kinases: a team that plays throughout mitosis
    D M Glover
    Department of Anatomy and Physiology, Medical Sciences Institute, University of Dundee, UK
    Genes Dev 12:3777-87. 1998
  67. doi Gene expression profiles of single human mature oocytes in relation to age
    M L Grøndahl
    University Hospital Copenhagen, Rigshospitalet, Fertility Clinic, Copenhagen, Denmark
    Hum Reprod 25:957-68. 2010
    ..The development competence of human oocytes declines with increasing age. The objective of this study was to investigate the effect of age on gene expression profile in mature human oocytes...
  68. pmc CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase
    T J Yen
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, MD 21205
    EMBO J 10:1245-54. 1991
    ..This association with chromosomes remains throughout metaphase but is redistributed to the midplate at or just after the onset of anaphase...
  69. ncbi PtdIns(3,4,5)P3 regulates spindle orientation in adherent cells
    Fumiko Toyoshima
    Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Dev Cell 13:796-811. 2007
    ..In metaphase, PtdIns(3,4,5)P3 is accumulated in the midcortex in an integrin-dependent manner...
  70. pmc Spindle checkpoint protein Xmad1 recruits Xmad2 to unattached kinetochores
    R H Chen
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    J Cell Biol 143:283-95. 1998
    The spindle checkpoint prevents the metaphase to anaphase transition in cells containing defects in the mitotic spindle or in chromosome attachment to the spindle...
  71. ncbi BubR1 is a spindle assembly checkpoint protein regulating meiotic cell cycle progression of mouse oocyte
    Liang Wei
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Science, Beijing, China
    Cell Cycle 9:1112-21. 2010
    ..The expression level of BubR1 increased progressively from germinal vesicle to metaphase II stages...
  72. pmc Mouse Emi2 is required to enter meiosis II by reestablishing cyclin B1 during interkinesis
    Suzanne Madgwick
    Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle, Newcastle NE2 4HH, England, UK
    J Cell Biol 174:791-801. 2006
    During interkinesis, a metaphase II (MetII) spindle is built immediately after the completion of meiosis I. Oocytes then remain MetII arrested until fertilization...
  73. doi Microsatellite accumulation on the Y chromosome in Silene latifolia
    Zdenek Kubat
    Laboratory of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    Genome 51:350-6. 2008
    ....
  74. pmc Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage
    Xing Zeng
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 18:382-95. 2010
    ..A prodrug of TAME arrests cells in metaphase without perturbing the spindle, but nonetheless the arrest is dependent on the SAC...
  75. ncbi Mph1, a member of the Mps1-like family of dual specificity protein kinases, is required for the spindle checkpoint in S. pombe
    X He
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    J Cell Sci 111:1635-47. 1998
    ..checkpoint pathway is not essential for normal mitosis but ensures accurate nuclear division by blocking the metaphase to anaphase transition in response to a defective spindle...
  76. pmc Mutations in the chromosomal passenger complex and the condensin complex differentially affect synaptonemal complex disassembly and metaphase I configuration in Drosophila female meiosis
    Tamar D Resnick
    Whitehead Institute for Biomedical Research, and the Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Genetics 181:875-87. 2009
    ..a role for dcap-g in disassembly of the synaptonemal complex and for proper retention of the chromosomes in a metaphase I-arrested state...
  77. pmc The Y-encoded gene zfy2 acts to remove cells with unpaired chromosomes at the first meiotic metaphase in male mice
    Nadège Vernet
    Division of Stem Cell Biology and Developmental Genetics, Medical Research Council National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
    Curr Biol 21:787-93. 2011
    ..there is efficient apoptotic elimination of cells with unpaired (univalent) chromosomes at the first meiotic metaphase (MI) [1]...
  78. pmc The Mos/mitogen-activated protein kinase (MAPK) pathway regulates the size and degradation of the first polar body in maturing mouse oocytes
    T Choi
    ABL Basic Research Program, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 93:7032-5. 1996
    Mos is an upstream activator of mitogen-activated protein kinase (MAPK) and, in mouse oocytes, is responsible for metaphase II arrest. This activity has been likened to its function in Xenopus oocytes as a component of cytostatic factor...
  79. pmc Metaphase arrest with centromere separation in polo mutants of Drosophila
    M M Donaldson
    Cancer Research Campaign Cell Cycle Genetics Research Group, Department of Anatomy and Physiology, University of Dundee, Dundee DD1 4HN, Scotland
    J Cell Biol 153:663-76. 2001
    ..Here we report two strongly hypomorphic mutations of polo that arrest cells of the larval brain at a point in metaphase when the majority of sister kinetochores have separated by between 20-50% of the total spindle length in intact ..
  80. ncbi S. cerevisiae 26S protease mutants arrest cell division in G2/metaphase
    M Ghislain
    Departement de Biologie Cellulaire et Moleculaire, Centre d Etudes de Saclay, Gif sur Yvette, France
    Nature 366:358-62. 1993
    ..isolated two mutants from the yeast Saccharomyces cerevisiae, cim3-1 and cim5-1, that arrest cell division in G2/metaphase at 37 degrees C. CIM3 (identical to SUG1; ref...
  81. ncbi Ca(2+) oscillations promote APC/C-dependent cyclin B1 degradation during metaphase arrest and completion of meiosis in fertilizing mouse eggs
    Victoria L Nixon
    Department of Physiological Sciences, The Medical School, Framlington Place, University of Newcastle, Newcastle NE2 4HH, United Kingdom
    Curr Biol 12:746-50. 2002
    Cyclin B1, the regulatory component of M phase-promoting factor (MPF), is degraded during the metaphase-anaphase transition in an anaphase-promoting complex/cyclosome (APC/C)-dependent process...
  82. pmc Essential role of protein phosphatase 2A in metaphase II arrest and activation of mouse eggs shown by okadaic acid, dominant negative protein phosphatase 2A, and FTY720
    Heng Yu Chang
    School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, New South Wales, 2308, Australia
    J Biol Chem 286:14705-12. 2011
    Vertebrate eggs arrest at second meiotic metaphase. The fertilizing sperm causes meiotic exit through Ca(2+)-mediated activation of the anaphase-promoting complex/cyclosome (APC/C)...
  83. pmc Mammalian Emi2 mediates cytostatic arrest and transduces the signal for meiotic exit via Cdc20
    Shisako Shoji
    Laboratory of Mammalian Molecular Embryology, RIKEN Center for Developmental Biology, Chuo Ku, Kobe, Japan
    EMBO J 25:834-45. 2006
    Fertilizable mammalian oocytes are arrested at the second meiotic metaphase (mII) by the cyclinB-Cdc2 heterodimer, maturation promoting factor (MPF)...
  84. pmc Prophase I arrest and progression to metaphase I in mouse oocytes: comparison of resumption of meiosis and recovery from G2-arrest in somatic cells
    Petr Solc
    Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Rumburska 89, Libechov CZ 27721, Czech Republic
    Mol Hum Reprod 16:654-64. 2010
    ..These common features include CDC14B-dependent activation of APC-CDH1 in prophase I arrested oocytes or G2-arrested somatic cells, and CDC25B-dependent cell cycle resumption in both oocytes and somatic cells...
  85. pmc Dynamics of human DNA topoisomerases IIalpha and IIbeta in living cells
    Morten O Christensen
    Department of Clinical Chemistry, Medizinische Poliklinik, University of Wurzburg, D 97070 Wurzburg, Germany
    J Cell Biol 157:31-44. 2002
    ..These observations suggest that topo II is not an immobile, structural component of the chromosomal scaffold or the interphase karyoskeleton, but rather a dynamic interaction partner of such structures...
  86. ncbi INCENP binds directly to tubulin and requires dynamic microtubules to target to the cleavage furrow
    S P Wheatley
    Wellcome Institute for Cell Biology, Institute of Cell and Molecular Biology, Swann Building, King s Buildings, Mayfield Road, Edinburgh, EH9 3JR, Scotland
    Exp Cell Res 262:122-7. 2001
    Inner centromere protein (INCENP) is a chromosomal passenger protein with an essential role in mitosis. At the metaphase/anaphase transition, some INCENP transfers from the centromeres to the central spindle; the remainder then transfers ..
  87. pmc RAD51C deficiency in mice results in early prophase I arrest in males and sister chromatid separation at metaphase II in females
    Sergey Kuznetsov
    Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA
    J Cell Biol 176:581-92. 2007
    ..In contrast, oocytes can progress normally to metaphase I after superovulation but display precocious separation of sister chromatids, aneuploidy, and broken ..
  88. pmc Automated classification of metaphase chromosomes: optimization of an adaptive computerized scheme
    Xingwei Wang
    Center for Bioengineering and School of Electrical and Computer Engineering, University of Oklahoma, 202 West Boyd Street, Room 219, Norman, OK 73019, USA
    J Biomed Inform 42:22-31. 2009
    ..The maximum difference of classification accuracy between the testing and validation datasets is <1.7%. The study demonstrates that this new scheme achieves higher and robust performance in classifying chromosomes...
  89. pmc Zwint-1 is a novel Aurora B substrate required for the assembly of a dynein-binding platform on kinetochores
    James M Kasuboski
    Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
    Mol Biol Cell 22:3318-30. 2011
    ..However, cells expressing the triple-Glu mutant failed to satisfy the spindle assembly checkpoint (SAC) at metaphase because poleward streaming of dynein/dynactin/RZZ was inhibited...
  90. doi Enrichment of cell populations in metaphase, anaphase, and telophase by synchronization using nocodazole and blebbistatin: a novel method suitable for examining dynamic changes in proteins during mitotic progression
    Yuki Matsui
    Department of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260 8675, Japan
    Eur J Cell Biol 91:413-9. 2012
    Mitosis is a continuous process to separate replicated chromosomes into two daughter cells through prophase, metaphase, anaphase, and telophase...
  91. ncbi Spindle assembly in Drosophila neuroblasts and ganglion mother cells
    S Bonaccorsi
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, P le Aldo Moro 5, 00185 Rome, Italy
    Nat Cell Biol 2:54-6. 2000
  92. ncbi A new chromosome model
    G Wanner
    Botanical Institute of LMU Munich, Menzinger Str 67, D 80638 Munich, Germany
    J Struct Biol 132:147-61. 2000
    ..With DNA and protein staining it could be shown by high-resolution scanning electron microscopy that metaphase chromosomes are mainly composed of DNA packed in "chromomeres" (coiled solenoides) and a dynamic matrix formed ..
  93. pmc PI 3-kinase-dependent phosphorylation of Plk1-Ser99 promotes association with 14-3-3γ and is required for metaphase-anaphase transition
    Kousuke Kasahara
    Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi 464 8681, Japan
    Nat Commun 4:1882. 2013
    ..of 14-3-3γ or replacement of wild-type (WT) Plk1 by a Ser99-phospho-blocking mutant leads to a prometaphase/metaphase-like arrest due to the activation of the spindle assembly checkpoint...
  94. ncbi Sequential dephosphorylation of p34(cdc2) on Thr-14 and Tyr-15 at the prophase/metaphase transition
    A Borgne
    Centre National de la Recherche Scientifique, Station Biologique, BP 74, 29682 Roscoff Cedex, France
    J Biol Chem 271:27847-54. 1996
    The G2-M transition of the cell cycle is triggered by the p34(cdc2)/cyclin B kinase. During the prophase/metaphase transition, the inactive, Thr-14/Tyr-15 phosphorylated form of p34(cdc2) (TP-YP) is modified to an active, Thr-14/Tyr-15 ..
  95. pmc Nondisjunctional segregations in Drosophila female meiosis I are preceded by homolog malorientation at metaphase arrest
    Shane C Gillies
    Department of Biological Sciences, DePaul University, Chicago IL 60614, USA
    Genetics 193:443-51. 2013
    ..model of Drosophila female meiosis I was recently revised by the discovery that chromosome congression precedes metaphase I arrest...
  96. doi Chronic exposure to a low concentration of bisphenol A during follicle culture affects the epigenetic status of germinal vesicles and metaphase II oocytes
    Tom Trapphoff
    Institute of Gene Technology Microbiology, University of Bielefeld, Bielefeld, Germany
    Fertil Steril 100:1758-67.e1. 2013
    ....
  97. ncbi Degradation of securin in mouse and pig oocytes is dependent on ubiquitin-proteasome pathway and is required for proteolysis of the cohesion subunit, Rec8, at the metaphase-to-anaphase transition
    Li Jun Huo
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Front Biosci 11:2193-202. 2006
    Although securin/separase/cohesion pathway was reported to regulate chromosome segregation during meiotic metaphase-to-anaphase transition, little biochemical evidence was provided...
  98. pmc Clinical benefit of metaphase I oocytes
    Leen Vanhoutte
    Infertility Centre, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium
    Reprod Biol Endocrinol 3:71. 2005
    We studied the benefit of using in vitro matured metaphase I (MI) oocytes for ICSI in patients with a maximum of 6 mature metaphase II (MII) oocytes at retrieval.
  99. pmc Developmental potential and ultrastructural injuries of metaphase II (MII) mouse oocytes after slow freezing or vitrification
    Mojtaba Rezazadeh Valojerdi
    Department of Anatomy, School of Medical Sciences, Tarbiat Modarres University, P O BOX 14115 111, Tehran, Iran
    J Assist Reprod Genet 22:119-27. 2005
    ..The purpose of this study was to determine the developmental ability and ultrastructure of MII mouse oocytes after cryopreservation by slow freezing or vitrification...
  100. ncbi The outcome of ICSI of immature MI oocytes and rescued in vitro matured MII oocytes
    D Strassburger
    Assaf Harofeh Medical Center, Tel Aviv University, Zerifin, 70300, Israel
    Hum Reprod 19:1587-90. 2004
    ..This study compares the outcome of injection of sperm into metaphase I oocytes immediately after their denudation (MI) performed 2 h after their retrieval, with the outcome of ..
  101. pmc Prophase I arrest and progression to metaphase I in mouse oocytes are controlled by Emi1-dependent regulation of APC(Cdh1)
    Petros Marangos
    Department of Physiology, University College London, London WC1E 6BT, England, UK
    J Cell Biol 176:65-75. 2007
    ..Finally, we show that Emi1-dependent effects on meiosis I require the presence of Cdh1. These observations reveal a novel mechanism for the control of entry into the first meiotic division: an Emi1-dependent inhibition of APC(Cdh1)...

Research Grants64

  1. Functional Analysis of the Dual Specificity Kinase NEK1 in Mammalian Meiosis
    JOANNA KIM HOLLOWAY; Fiscal Year: 2011
    ..The central hypothesis of this proposal is that NEK1 is required for prophase I to metaphase progression, as it links key SC events with those involving sister chromatid cohesion...
  2. Cell Cycle Regulation and Adult T Cell Leukemia
    Chou Zen Giam; Fiscal Year: 2013
    ..that Tax can activate the anaphase promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase that controls metaphase to anaphase transition and mitotic exit...
  3. RADIATION-INDUCED THYROID CANCER
    Heinz Ulrich G Weier; Fiscal Year: 2011
    ..the presence or absence of these translocations in 20 additional cases of chPTC for which we already archived metaphase spreads...
  4. Molecular Pathogenesis of MDS and CMML
    Jaroslaw P Maciejewski; Fiscal Year: 2013
    ..However, using traditional metaphase cytogenetics (MC), chromosomal lesions can be found in only about 50% of patients;higher resolution methods may ..
  5. Regulation of EBV Latency by EBNA1
    Paul M Lieberman; Fiscal Year: 2013
    ..sequence-specific DNA-binding protein that regulates viral DNA replication, episome maintenance, metaphase chromosome tethering, and transcription. EBNA1 is also required for host-cell survival...
  6. ATM Controls Genomic Stability in Pluripotent Stem Cells
    JASON MARK BECKTA; Fiscal Year: 2013
    ..cell imaging studies (accomplished preliminarily in non-transformed normal fibroblasts) revealed a prolonged metaphase-to-anaphase transition time after ATM inhibition...
  7. Functional Analysis of the Dual Specificity Kinase NEK1 in Mammalian Meiosis
    JOANNA KIM HOLLOWAY; Fiscal Year: 2013
    ..The central hypothesis of this proposal is that NEK1 is required for prophase I to metaphase progression, as it links key SC events with those involving sister chromatid cohesion...
  8. ANALYSIS OF MEIOTIC CHROMOSOME SYNAPSIS IN YEAST
    Nancy M Hollingsworth; Fiscal Year: 2013
    ..To promote proper orientation at Metaphase I of meiosis, homologous chromosomes are physically connected by a combination of sister chromatid cohesion and ..
  9. The Role of Cks Proteins in Mammalian Meiosis
    Charles H Spruck; Fiscal Year: 2010
    ..CKS2-/- male and female mice are sterile due to an arrest of spermatocytes and oocytes in metaphase of meiosis I (Ml)...
  10. HTLV-I Tax activates the anaphase promoting complex
    Chou Zen Giam; Fiscal Year: 2013
    ..that the Tax can activate the anaphase promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase that controls metaphase to anaphase transition and mitotic exit...
  11. Training/Research Center-Bone Marrow Failure Syndromes
    Jaroslaw P Maciejewski; Fiscal Year: 2013
    ..We propose that single nucleotide polymorphisms arrays (SNP-A) can be applied, complementary to metaphase cytogenetics for the identification of chromosomal abnormalities, including a newly recognized class of lesion, ..
  12. CONTROL OF MEIOSIS AND GERMLINE PROLIFERATION
    DAVID IRWIN GREENSTEIN; Fiscal Year: 2012
    ..Meiotic maturation is defined by the transition between diakinesis and metaphase of meiosis I and is accompanied by nuclear envelope breakdown, cortical cytoskeletal rearrangement, and meiotic ..
  13. Establishment of sister chromatid cohesion
    Douglas E Koshland; Fiscal Year: 2013
    ..the two sister chromatids (the newly replicated chromosomes) from the time of their synthesis in S phase through metaphase of mitosis...
  14. Mitochondrial Gene Therapy
    Shoukhrat M Mitalipov; Fiscal Year: 2013
    ..e., spindle transfer (ST) in mature metaphase II (MII) oocytes without interfering with subsequent nucleo-mtDNA compatibility and developmental competence...
  15. Meiotic spindle formation in Drosophila females
    Kim S McKim; Fiscal Year: 2010
    ..Subito is required for the development of the central spindle at meiotic metaphase and this structure may be critical for formation of a bipolar spindle in the absence of centrosomes...
  16. Role of Cyclin A's in mammalian gametogenesis
    Debra J Wolgemuth; Fiscal Year: 2013
    ..and by targeted mutagenesis, that it is essential for spermatogenesis--spermatocytes arrest at the diplotene to metaphase 1 transition...
  17. Kinetochore Function and Cell Cycle Progression Revision
    Katsumi Kitagawa; Fiscal Year: 2012
    ..These results imply that the accumulation of phosphorylated Bub1 during metaphase is the signal that initiates the silencing of spindle checkpoint activity after a prolonged mitotic arrest...
  18. Accelerating Cancer Research with Single Cell Arrays
    Heinz Ulrich Weier; Fiscal Year: 2009
    ..Current techniques for full karyotype analysis rely on metaphase spreads, and cells in interphase cannot be analyzed...
  19. Regulation of meiotic recombination and chromosome segregation in mammals
    KAREN MICHELE BERKOWITZ; Fiscal Year: 2013
    ..subfertile, homologues separately prematurely during meiosis I, and progression of Chtf18-null oocytes through metaphase II is impaired. Thus, our data demonstrate essential roles for Chtf18 in mammalian gametogenesis and meiosis...
  20. Molecular Mechanisms of Meiotic Maturation in Drosophila
    Daniela Drummond-Barbosa; Fiscal Year: 2013
    ..After meiotic maturation, oocytes arrest again in metaphase I until ovulation...
  21. Mechanism controlling centrosome duplication
    Barbara E Tanos; Fiscal Year: 2013
    ..In Aim 1, I will employ a two-step disengagement assay using Xenopus egg extracts to examine whether Plk1 metaphase activity is required for disengagement...
  22. Regulation of Mammalian Oocyte Maturation
    Stephen M Downs; Fiscal Year: 2010
    ..In Specific Aim 1, we will determine the influence of AMPK from prophase I to Metaphase II and the association of the kinase with chromosomes and microtubules...
  23. Protection of Centromeric Cohesion by Bub1 and Sgo1
    Hongtao Yu; Fiscal Year: 2010
    ..At metaphase, the residual centromeric pool of cohesin is cleaved by separase to allow sister chromatid separation...
  24. A novel mouse colon cancer model and chemoprevention
    Wei Dai; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): The spindle checkpoint delays the progression from metaphase to anaphase until all condensed chromosomes are properly attached to mitotic spindles...
  25. Tubulin-Binding Upconversion Nanoparticles for Breast-Cancer Imaging and Therapy
    Chuanbin Mao; Fiscal Year: 2013
    ..agents, the mitotic spindle functions will be perturbed, resulting in the inhibition of cell division at the metaphase/anaphase transition of mitosis...
  26. FEEDBACK CONTROL OF THE CELL CYCLE
    Andrew W Murray; Fiscal Year: 2013
    ..kinetochores will reveal what determines the number of microtubules in the spindle, the length of the spindle in metaphase, the rate at which kinetochores attach to and detach from spindle microtubules, and the activity of the spindle ..
  27. Structure and Function of a Eukaryotic Centromere
    Kerry S Bloom; Fiscal Year: 2013
    ..barrel structure composed of cohesin, condensin and pericentric DNA that encompasses the spindle microtubules in metaphase in the model organism, S. cerevisiae [1]...
  28. Mechanisms of the Unusual Cytokinesis in Trypanosomes
    Ziyin Li; Fiscal Year: 2013
    ..a dynamic localization during mitosis and cytokinesis by migrating from chromatins to the central spindle during metaphase-anaphase transition and from the central spindle to the anterior tip of the new FAZ during mitosis-cytokinesis ..
  29. Regulation of Chromosome Segregation
    DAVID OWEN MORGAN; Fiscal Year: 2013
    ..During mitosis, the sister-chromatid pairs are oriented on the bipolar mitotic spindle. At the metaphase-anaphase transition, the cohesin linkage between sister's chromatids is abruptly dissolved by a protease ..
  30. Motor Protein Dynamics and Mitotic Mechanisms
    Tarun M Kapoor; Fiscal Year: 2013
    ..We will use a dual force-calibrated microneedle-based system to examine the viscoelastic properties of the metaphase spindle...
  31. Gene mutation and rescue in human diaphragmatic hernia
    Patricia K Donahoe; Fiscal Year: 2010
    ..genes, arrayed based Comparative Genomic Hybridization (aCGH), loss of heterozygosity studies (LOH), and metaphase preparations for subtelomeric FISH...
  32. Mechanisms of Spindle Assembly
    Claire E Walczak; Fiscal Year: 2012
    ..One of the most complex aspects of mitosis is the congression of chromosomes to the metaphase plate, which involves a multitude of forces acting on the kinetochore, on the chromosome arms, and on the ..
  33. Formin mDia Functions in Mitosis
    Yinghui Mao; Fiscal Year: 2013
    ..We have shown that knockdown of mDia3 in mammalian cultured cells using siRNA results in defects in metaphase chromosome alignment and stable kinetochore microtubule attachment...
  34. BIOCHEMICAL AND GENETIC ANALYSIS OF YEAST KINETOCHORES
    PETER KARL SORGER; Fiscal Year: 2010
    ..move chromosomes back and forth along spindle MTs by coupling plus-end dynamics to chromosome movement during metaphase and anaphase (iii) they generate the spindle checkpoint signal that links anaphase onset to the prior completion ..
  35. alpha-endosulfine in mammalian oocyte meiotic maturation
    Janice P Evans; Fiscal Year: 2012
    ..with endos-deficient oocytes having defects in progression from prophase I arrest and in organization of the metaphase I spindle (Development 135:3697)...
  36. Structural Annotation of the Human Genome
    Job Dekker; Fiscal Year: 2013
    ..to long-range gene regulation, chromosome segregation, the epigenetic transmission of transcription profiles to daughter cells, and the still largely mysterious process of formation of compact metaphase chromosomes.
  37. KINETOCHORE STRUCTURE AND FUNCTION
    TIMOTHY YEN; Fiscal Year: 2010
    ..cause cells to accumulate aberrant kinetochore attachments that fail to generate tension and thus delay at metaphase. Failure to correct these defective attachments by the time cells overcome the mitotic delay results in lagging ..
  38. The Micromechanics of Central Spindle Organization
    SCOTT THOMAS FORTH; Fiscal Year: 2013
    ..of cell division is the self-organized assemblage of microtubules which adopts a bipolar configuration in metaphase and a central spindle upon entry into anaphase...
  39. ORGANIZATION OF THE MAMMALIAN MITOTIC SPINDLE
    Duane A Compton; Fiscal Year: 2013
    ..To use live cell imaging to determine the biochemical changes underlying the transition from prometaphase to metaphase;2...
  40. Regeneration of meiotic spindle during oocyte vitrification
    Yusuke Marikawa; Fiscal Year: 2013
    ..In Specific Aim 3, the stability of metaphase chromosomes in the absence of spindle will be investigated...
  41. Regulation of the Anaphase-Promoting Complex by the Spindle Checkpoint
    Hongtao Yu; Fiscal Year: 2010
    ..APC/C) is a large multi-subunit ubiquitin ligase that controls several cell cycle transitions, including the metaphase-anaphase transition...
  42. Colf NRSA Roles of ESCRT-III/VPS4 Proteins in Mitosis
    Leremy A Colf; Fiscal Year: 2011
    ..early in mitosis, and that their depletion gives rise to aberrant centrosome numbers, spindle assembly, and metaphase chromosome alignment. Thus, the ESCRT pathway functions at multiple stages of cell division...
  43. MECHANISMS OF MICTOTIC SPINDLE ASSEMBLY AND FUNCTION
    Edward D Salmon; Fiscal Year: 2013
    ..to delay anaphase until sister kinetochores are properly attached by MTs to opposite poles and aligned on the metaphase plate;2) they provide stable, but dynamic, attachment to MT plus ends to turn off spindle checkpoint activity ..
  44. The SMC5/6 Complex - DNA Damage Response Regulation Ensures Meiotic Fidelity
    Philip W Jordan; Fiscal Year: 2013
    ..This function ensures fidelity at the prophase to metaphase I transition of meiosis.
  45. The SMC5/6 Complex - DNA Damage Response Regulation Ensures Meiotic Fidelity
    Philip W Jordan; Fiscal Year: 2012
    ..This function ensures fidelity at the prophase to metaphase I transition of meiosis.
  46. A computer aided chromosome imaging technique for cancer diagnosis
    Hong Liu; Fiscal Year: 2010
    ..it takes tremendous effort and time for a cytogenetic clinician to obtain a sufficient number of analyzable metaphase cells under microscope before he/she can make an accurate clinical diagnosis...
  47. A Systems Biology Analysis of Spindle Checkpoint Signaling
    Xuedong Liu; Fiscal Year: 2010
    ..attached kinetochore is sufficient to block cell cycle progression and generate sustainable mitotic arrest at metaphase. Once proper kinetochore attachment is achieved, checkpoint signaling is extinguished allowing the cells exit ..
  48. Determining the spindle dynamics regulatory network with an integrated approach
    Ao Ma; Fiscal Year: 2013
    ..proteins (KLP59C, KLP67A, Mast, EB1 and Msps) that governs kinetochore MT (kMT) plus-end dynamics during metaphase. This network utilizes a complex balance between MT polymerases and depolymerases (instead of polymerases alone) ..
  49. Role of Mitotic Checkpoint Defects in Mouse Tumor Models
    ROBERT I BENEZRA; Fiscal Year: 2010
    ..Mad2 is a component of the machinery which arrests a cell prior to the metaphase to anaphase transition if all the chromosomes are not attached to the mitotic spindle apparatus...
  50. FLUORESCENT GENE MAPPING AT INTERPHASE & METAPHASE
    Jeanne Lawrence; Fiscal Year: 1993
    ..This method provides significant advantages in resolution, speed and convenience for metaphase chromosome mapping as well, which will be demonstrated by precise metaphase localization of specific cellular ..
  51. S POMBE PROTEIN/CENTROMERIC DNA INTERACTION
    Jerard Hurwitz; Fiscal Year: 2001
    ..forms and it is the last site by which sister chromatids remain associated prior to their separation at the metaphase-anaphase transition...
  52. Function of the chromosomal kinase haspin in mitosis
    JONATHAN M HIGGINS; Fiscal Year: 2010
    ..Importantly, haspin RNAi causes misalignment of metaphase chromosomes and spindle defects, and overexpression delays progression through early mitosis...
  53. ZYGOTIC DNA REPAIR OF ENVIRONMENT INDUCED SPERM LESIONS
    ANDREW WYROBEK; Fiscal Year: 2000
    ..of male mice to mutagens before mating can induce high frequencies of chromosomal defects detected at zygotic metaphase and that the incidence depends on the genotype of the egg...
  54. BIMA AND MITOSIS
    PETER MIRABITO; Fiscal Year: 1999
    The metaphase to anaphase transition is a complex and critical step in mitosis...
  55. SYNTHESIS OF TAXININE AND TAXOL
    CHARLES SWINDELL; Fiscal Year: 1992
    ..Through this affect, taxol treated cells are blocked in metaphase and cellular replication is inhibited...
  56. WHY IS HISTONE ACETYLATION REDUCED AT MITOSIS?
    James Paulson; Fiscal Year: 1992
    ..acetylation at certain lysine residues, but this acetylation is drastically reduced during normal mitosis and in metaphase-arrested cells...
  57. G2 CHECKPOINT FUNCTION IN HUMAN FIBROBLASTS
    William Kaufmann; Fiscal Year: 2007
    ..Override of the ICRF-193-induced G2 delay caused the appearance of constrictions between chromatid arms in metaphase spreads, suggesting the presence of linkages or catenations due to inhibition of topoll...
  58. MOLECULAR CYTOGENETICS IN PRE AND NEONATAL DIAGNOSIS
    Daniel Pinkel; Fiscal Year: 1999
    ..In this approach, CGH with differentially labeled test and normal reference DNA to normal metaphase spreads (termed CGHx) will be used to identify aneusomies...
  59. Control of Genomic Stability by Emi1 and Securin
    NORMAN LEHMAN; Fiscal Year: 2007
    ..chromosomes to daughter cells by inhibiting anaphase progression until all chromosomes are properly aligned at metaphase. When cells are manipulated to over-or under-express securin they develop chromosomal abnormalities and ..
  60. FUNCTION DOMAINS IN THE NUCLEAR LAMIN PROTEINS
    Frank McKeon; Fiscal Year: 1992
    ..proteins that function in nuclear transport, assembly into the nuclear envelope, processing, and disassembly in metaphase. In addition, experiments will be performed to uncover the role of the nuclear lamins in nuclear pore ..
  61. The Anaphase Promoting Complex and Cell Cycle Exit
    Nagi G Ayad; Fiscal Year: 2013
    ..The APC controls both the metaphase to anaphase transition and mitotic exit...
  62. Analysis of Loss of Function of MAD2 in Mammals
    Loren Michel; Fiscal Year: 2006
    ..The mitotic checkpoint delays mitosis at metaphase until the attachment of microtubules to the kinetochores is complete, allowing for equal segregation of ..
  63. METAPHASE TO ANAPHASE TRANSITION IN MITOSIS AND MEIOSIS
    Diane Shakes; Fiscal Year: 2003
    ..chromosomes are replicated during S-phase, compacted and restructured during prophase, and aligned during metaphase. As cells subsequently transition from metaphase-to-anaphase, the replicated chromosomes are separated and ..
  64. Control of Meiotic Homolog Segregation in Arabidopsis
    Hong Ma; Fiscal Year: 2004
    ..meiotic recombination and sister chromatid cohesion are required for homolog attachment at late prophase I and metaphase I...