meiosis

Summary

Summary: A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells.

Top Publications

  1. ncbi Meiotic sex chromosome inactivation
    James M A Turner
    Division of Stem Cell Biology and Developmental Genetics, MRC NIMR, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Development 134:1823-31. 2007
  2. ncbi Mechanism of eukaryotic homologous recombination
    Joseph San Filippo
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA
    Annu Rev Biochem 77:229-57. 2008
  3. ncbi Fine-scale recombination rate differences between sexes, populations and individuals
    Augustine Kong
    deCODE Genetics, Sturlugata 8, 101 Reykjavik, Iceland
    Nature 467:1099-103. 2010
  4. ncbi PRDM9 is a major determinant of meiotic recombination hotspots in humans and mice
    F Baudat
    Institut de Genetique Humaine, UPR1142, CNRS, Montpellier, France
    Science 327:836-40. 2010
  5. pmc COSA-1 reveals robust homeostasis and separable licensing and reinforcement steps governing meiotic crossovers
    Rayka Yokoo
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 149:75-87. 2012
  6. pmc Regulatory control of the resolution of DNA recombination intermediates during meiosis and mitosis
    Joao Matos
    London Research Institute, Cancer Research UK, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
    Cell 147:158-72. 2011
  7. ncbi Crossover/noncrossover differentiation, synaptonemal complex formation, and regulatory surveillance at the leptotene/zygotene transition of meiosis
    G Valentin Börner
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Cell 117:29-45. 2004
  8. pmc Drive against hotspot motifs in primates implicates the PRDM9 gene in meiotic recombination
    Simon Myers
    Department of Statistics, Oxford University, 1 South Parks Road, Oxford OX1 3TG, UK
    Science 327:876-9. 2010
  9. pmc Delineation of joint molecule resolution pathways in meiosis identifies a crossover-specific resolvase
    Kseniya Zakharyevich
    Department of Microbiology, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA
    Cell 149:334-47. 2012
  10. pmc Genetic recombination is directed away from functional genomic elements in mice
    Kevin Brick
    National Institute of Diabetes, Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892, USA
    Nature 485:642-5. 2012

Detail Information

Publications341 found, 100 shown here

  1. ncbi Meiotic sex chromosome inactivation
    James M A Turner
    Division of Stem Cell Biology and Developmental Genetics, MRC NIMR, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Development 134:1823-31. 2007
    ..However, another form of X-inactivation takes place in the male, during spermatogenesis, as germ cells enter meiosis. This second form of X-inactivation, called meiotic sex chromosome inactivation (MSCI) has emerged as a novel ..
  2. ncbi Mechanism of eukaryotic homologous recombination
    Joseph San Filippo
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA
    Annu Rev Biochem 77:229-57. 2008
    ..also critical for the preservation of replication forks, for telomere maintenance, and chromosome segregation in meiosis I. As such, HR is indispensable for the maintenance of genome integrity and the avoidance of cancers in humans...
  3. ncbi Fine-scale recombination rate differences between sexes, populations and individuals
    Augustine Kong
    deCODE Genetics, Sturlugata 8, 101 Reykjavik, Iceland
    Nature 467:1099-103. 2010
    ....
  4. ncbi PRDM9 is a major determinant of meiotic recombination hotspots in humans and mice
    F Baudat
    Institut de Genetique Humaine, UPR1142, CNRS, Montpellier, France
    Science 327:836-40. 2010
    ..Our results provide a molecular basis for the distribution of meiotic recombination in mammals, in which the binding of PRDM9 to specific DNA sequences targets the initiation of recombination at specific locations in the genome...
  5. pmc COSA-1 reveals robust homeostasis and separable licensing and reinforcement steps governing meiotic crossovers
    Rayka Yokoo
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 149:75-87. 2012
    Crossovers (COs) between homologous chromosomes ensure their faithful segregation during meiosis. We identify C...
  6. pmc Regulatory control of the resolution of DNA recombination intermediates during meiosis and mitosis
    Joao Matos
    London Research Institute, Cancer Research UK, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
    Cell 147:158-72. 2011
    ..Here, we show that the specialized chromosome segregation patterns of meiosis and mitosis, which require the coordination of recombination with cell-cycle progression, are achieved by ..
  7. ncbi Crossover/noncrossover differentiation, synaptonemal complex formation, and regulatory surveillance at the leptotene/zygotene transition of meiosis
    G Valentin Börner
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Cell 117:29-45. 2004
    ..Thus, during wild-type meiosis, recombinational interactions are differentiated into CR and NCR types very early, prior to onset of stable ..
  8. pmc Drive against hotspot motifs in primates implicates the PRDM9 gene in meiotic recombination
    Simon Myers
    Department of Statistics, Oxford University, 1 South Parks Road, Oxford OX1 3TG, UK
    Science 327:876-9. 2010
    ..The involvement of PRDM9, which causes histone H3 lysine 4 trimethylation, implies that there is a common mechanism for recombination hotspots in eukaryotes but raises questions about what forces have driven such rapid change...
  9. pmc Delineation of joint molecule resolution pathways in meiosis identifies a crossover-specific resolvase
    Kseniya Zakharyevich
    Department of Microbiology, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA
    Cell 149:334-47. 2012
    ..Using physical monitoring of recombination during budding yeast meiosis, we show that all three endonucleases are capable of promoting JM resolution in vivo...
  10. pmc Genetic recombination is directed away from functional genomic elements in mice
    Kevin Brick
    National Institute of Diabetes, Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892, USA
    Nature 485:642-5. 2012
    Genetic recombination occurs during meiosis, the key developmental programme of gametogenesis...
  11. ncbi Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family
    S Keeney
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Cell 88:375-84. 1997
    ..The S. pombe homolog, rec12p, is also known to be required for meiotic recombination. Thus, these findings provide direct evidence that the mechanism of meiotic recombination initiation is evolutionarily conserved...
  12. pmc Prdm9 controls activation of mammalian recombination hotspots
    Emil D Parvanov
    The Jackson Laboratory, Bar Harbor, ME 04609, USA
    Science 327:835. 2010
    ..remaining gene, Prdm9, codes for a zinc finger containing histone H3K4 trimethylase that is expressed in early meiosis and whose deficiency results in sterility in both sexes...
  13. pmc Bidirectional resection of DNA double-strand breaks by Mre11 and Exo1
    Valerie Garcia
    Genome Damage and Stability Centre, The University of Sussex, Brighton, BN1 9RQ, UK
    Nature 479:241-4. 2011
    ..Mre11 exonuclease activity also confers resistance to DNA damage in cycling cells, suggesting that Mre11-catalysed resection may be a general feature of various DNA repair pathways...
  14. ncbi Un ménage à quatre: the molecular biology of chromosome segregation in meiosis
    Mark Petronczki
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, 1030 Vienna, Austria
    Cell 112:423-40. 2003
    ..organisms rely on the precise reduction of chromosome number during a specialized cell division called meiosis. Whereas mitosis produces diploid daughter cells from diploid cells, meiosis generates haploid gametes from ..
  15. pmc Genome-wide analysis reveals novel molecular features of mouse recombination hotspots
    Fatima Smagulova
    Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    Nature 472:375-8. 2011
    ..Our findings uncover several fundamental features of mammalian recombination hotspots and underline the power of the new recombination map for future studies of genetic recombination, genome stability and evolution...
  16. ncbi DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression
    D K Bishop
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138
    Cell 69:439-56. 1992
    DMC1 is a new meiosis-specific yeast gene. Dmc1 protein is structurally similar to bacterial RecA proteins...
  17. ncbi Spo11-accessory proteins link double-strand break sites to the chromosome axis in early meiotic recombination
    Silvia Panizza
    Department of Chromosome Biology, Max F Perutz Laboratories, University of Vienna, Dr Bohr Gasse 1, A 1030 Vienna, Austria
    Cell 146:372-83. 2011
    ..Loss of Rec114, Mer2, and Mei4 binding correlates with loss of DSBs. Our results strongly suggest that hotspot sequences become tethered to axis sites by the DSB machinery prior to DSB formation...
  18. ncbi Meiotic chromosomes: integrating structure and function
    D Zickler
    Institut de Genetique et Microbiologie, Universite Paris Sud, Orsay, France
    Annu Rev Genet 33:603-754. 1999
    ..been studied for many years, in part because of the fundamental life processes they represent, but also because meiosis involves the formation of homolog pairs, a feature which greatly facilitates the study of chromosome behavior...
  19. ncbi To err (meiotically) is human: the genesis of human aneuploidy
    T Hassold
    Department of Genetics, Case Western Reserve University, 10, 900 Euclid Avenue, Cleveland, Ohio 44106, USA
    Nat Rev Genet 2:280-91. 2001
    ..However, recent molecular and cytogenetic approaches are now beginning to shed light on the non-disjunctional processes that lead to aneuploidy...
  20. ncbi Spp1, a member of the Set1 Complex, promotes meiotic DSB formation in promoters by tethering histone H3K4 methylation sites to chromosome axes
    Vérane Sommermeyer
    Institut Curie, Centre de Recherche, 75248 Paris, France
    Mol Cell 49:43-54. 2013
    ..Set1 complex, is required for normal levels of DSB formation and is associated with chromosome axes during meiosis, where it physically interacts with the Mer2 DSB protein...
  21. pmc Rad51 is an accessory factor for Dmc1-mediated joint molecule formation during meiosis
    Veronica Cloud
    Committee on Genetics, University of Chicago, Cummings Life Science Center, 920 East 58th Street, Chicago, IL 60637, USA
    Science 337:1222-5. 2012
    ..We further provide biochemical evidence that Rad51 acts with Mei5-Sae3 as a Dmc1 accessory factor. Thus, Rad51 is a multifunctional protein that catalyzes recombination directly in mitosis and indirectly, via Dmc1, during meiosis.
  22. pmc ATM controls meiotic double-strand-break formation
    Julian Lange
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA
    Nature 479:237-40. 2011
    ..Our findings explain previously puzzling phenotypes of Atm-null mice and provide a molecular basis for the gonadal dysgenesis observed in ataxia telangiectasia, the human syndrome caused by ATM deficiency...
  23. pmc Drosophila ATM and ATR have distinct activities in the regulation of meiotic DNA damage and repair
    Eric F Joyce
    Waksman Institute of Microbiology, Rutgers University, Piscataway, NJ 08854, USA
    J Cell Biol 195:359-67. 2011
    ..During meiosis, however, the functions of these kinases in DSB repair and the deoxyribonucleic acid (DNA) damage checkpoint are ..
  24. pmc Budding yeast ATM/ATR control meiotic double-strand break (DSB) levels by down-regulating Rec114, an essential component of the DSB-machinery
    Jesús A Carballo
    Department of Life Sciences, Genome Damage and Stability Centre, University of Sussex, Falmer, United Kingdom
    PLoS Genet 9:e1003545. 2013
    An essential feature of meiosis is Spo11 catalysis of programmed DNA double strand breaks (DSBs)...
  25. pmc Tet1 controls meiosis by regulating meiotic gene expression
    Shinpei Yamaguchi
    Howard Hughes Medical Institute, Harvard Medical School, WAB 149G, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 492:443-7. 2012
    b>Meiosis is a germ-cell-specific cell division process through which haploid gametes are produced for sexual reproduction...
  26. ncbi Meiotic catastrophe and retrotransposon reactivation in male germ cells lacking Dnmt3L
    Déborah Bourc'his
    Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, 701 West 168th Street, New York, New York 10032, USA
    Nature 431:96-9. 2004
    ..This result indicates that the Dnmt3L protein might have a function in the de novo methylation of dispersed repeated sequences in a premeiotic genome scanning process that occurs in male germ cells at about the time of birth...
  27. pmc Homeostatic control of recombination is implemented progressively in mouse meiosis
    Francesca Cole
    Developmental Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA
    Nat Cell Biol 14:424-30. 2012
    ..Meiotic recombination exemplifies how order can be progressively implemented in a self-organizing system despite natural cell-to-cell disparities in the underlying biochemical processes...
  28. pmc Double Holliday junctions are intermediates of DNA break repair
    Malgorzata Bzymek
    Department of Microbiology, University of California Davis, Davis, California 95616, USA
    Nature 464:937-41. 2010
    ..whose strand composition and size are identical to those of the canonical DHJ structures observed in meiosis. However, in contrast to meiosis, JMs between sister chromatids form in preference to those between homologues...
  29. pmc Cytoskeletal forces span the nuclear envelope to coordinate meiotic chromosome pairing and synapsis
    Aya Sato
    Howard Hughes Medical Institute, Chevy Chase, MD 20815 USA
    Cell 139:907-19. 2009
    During meiosis, each chromosome must pair with its unique homologous partner, a process that usually culminates with the formation of the synaptonemal complex (SC)...
  30. ncbi Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein
    A Shinohara
    Department of Biology, Faculty of Science, Osaka University, Japan
    Cell 69:457-70. 1992
    The RAD51 gene of S. cerevisiae is involved in mitotic recombination and repair of DNA damage and also in meiosis. We show that the rad51 null mutant accumulates meiosis-specific double-strand breaks (DSBs) at a recombination hotspot and ..
  31. ncbi The single-end invasion: an asymmetric intermediate at the double-strand break to double-holliday junction transition of meiotic recombination
    N Hunter
    Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Cell 106:59-70. 2001
    ..This feature can explain crossover suppression between homeologous and structurally heterozygous chromosomes...
  32. ncbi Differential timing and control of noncrossover and crossover recombination during meiosis
    T Allers
    Laboratory of Biochemistry, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Cell 106:47-57. 2001
    ..Contrary to this expectation, we find that during meiosis in Saccharomyces cerevisiae, noncrossover heteroduplex products are formed at the same time as Holliday junction ..
  33. pmc Meiosis: making a break for it
    Judith Yanowitz
    Magee Womens Research Institute, 204 Craft Avenue, Pittsburgh, PA 15213, USA
    Curr Opin Cell Biol 22:744-51. 2010
    ..b>Meiosis is a distinguishing feature of gamete formation as it leads to the twofold reduction in chromosome number thereby ..
  34. pmc PRDM9 variation strongly influences recombination hot-spot activity and meiotic instability in humans
    Ingrid L Berg
    Department of Genetics, University of Leicester, Leicester, UK
    Nat Genet 42:859-63. 2010
    ....
  35. pmc Retinoic acid regulates sex-specific timing of meiotic initiation in mice
    Jana Koubova
    Howard Hughes Medical Institute, and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 103:2474-9. 2006
    In mammals, meiosis is initiated at different time points in males and females, but the mechanism underlying this difference is unknown. Female germ cells begin meiosis during embryogenesis...
  36. ncbi Structural and functional relationships of the XPF/MUS81 family of proteins
    Alberto Ciccia
    London Research Institute, Cancer Research UK, Clare Hall Laboratories, Hertfordshire, United Kingdom
    Annu Rev Biochem 77:259-87. 2008
    ..Determining how these enzymes recognize specific DNA substrates and promote key repair reactions is an important challenge for the future...
  37. ncbi The choice in meiosis - defining the factors that influence crossover or non-crossover formation
    Jillian L Youds
    DNA Damage Response Laboratory, Cancer Research UK, London Research Institute, Clare Hall, Blanche Lane, South Mimms EN6 3LD, UK
    J Cell Sci 124:501-13. 2011
    ..During meiosis, excess meiotic double-strand breaks (DSBs) are generated; a subset of these breaks are repaired to form ..
  38. pmc Sister cohesion and structural axis components mediate homolog bias of meiotic recombination
    Keun P Kim
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Cell 143:924-37. 2010
    ..analyses, we show that sister cohesion, normally mediated by meiotic cohesin Rec8, promotes "sister bias"; that meiosis-specific axis components Red1/Mek1kinase counteract this effect, thereby satisfying an essential precondition for ..
  39. pmc Meiotic DNA double-strand breaks and chromosome asynapsis in mice are monitored by distinct HORMAD2-independent and -dependent mechanisms
    Lukasz Wojtasz
    Institute of Physiological Chemistry, Technische Universitat Dresden, Dresden 01307, Germany
    Genes Dev 26:958-73. 2012
    ..Enduring key questions in meiosis have been how meiotic progression and crossover formation are coordinated, whether inappropriate asynapsis is ..
  40. ncbi Generating crossovers by resolution of nicked Holliday junctions: a role for Mus81-Eme1 in meiosis
    Fekret Osman
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    Mol Cell 12:761-74. 2003
    ..These data demonstrate how crossovers could arise without forming or resolving dHJs using an enzyme that is widely conserved amongst eukaryotes...
  41. pmc Meiotic double-strand breaks occur once per pair of (sister) chromatids and, via Mec1/ATR and Tel1/ATM, once per quartet of chromatids
    Liangran Zhang
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 108:20036-41. 2011
    ..The two types of constraints on DSB formation provide two different safeguards against recombination failure during meiosis.
  42. ncbi Retinoid signaling determines germ cell fate in mice
    Josephine Bowles
    Division of Genetics and Developmental Biology, Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    Science 312:596-600. 2006
    ..We found that retinoic acid, produced by mesonephroi of both sexes, causes germ cells in the ovary to enter meiosis and initiate oogenesis...
  43. ncbi Synaptonemal complex assembly in C. elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination
    MONICA P COLAIACOVO
    Department of Developmental Biology, Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Dev Cell 5:463-74. 2003
    ..complex (SC) and progression of recombination between homologous chromosomes during Caenorhabditis elegans meiosis. We identify SYP-2 as a structural component of the SC central region and show that central region assembly ..
  44. pmc A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis
    Akihiro Morimoto
    Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113 0032, Japan
    J Cell Biol 198:165-72. 2012
    ..Thus, KASH5 is a mammalian meiosis-specific KASH domain protein...
  45. pmc Genome-wide analysis of heteroduplex DNA in mismatch repair-deficient yeast cells reveals novel properties of meiotic recombination pathways
    Emmanuelle Martini
    CEA DSV iRCM, Unité Mixte de Recherche 217 Radiobiologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, Commissariat à l Énergie Atomique et aux Énergies Alternatives, Fontenay aux Roses, France
    PLoS Genet 7:e1002305. 2011
    ..Finally, the good correlation between the strand transfer properties observed in the absence of and in the presence of Msh2 suggests that the intermediates detected in the absence of Msh2 reflect normal intermediates...
  46. ncbi The mouse Spo11 gene is required for meiotic chromosome synapsis
    P J Romanienko
    Genetics and Biochemistry Branch National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health 20892, Bethesda, MD, USA
    Mol Cell 6:975-87. 2000
    ..We speculate that there is an additional role for Spo11, after it generates DSBs, in synapsis...
  47. pmc Numerical constraints and feedback control of double-strand breaks in mouse meiosis
    Liisa Kauppi
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Genes Dev 27:873-86. 2013
    ..We conclude that homolog pairing requirements dictate DSB set points during meiosis. Importantly, our results reveal that karyotype is a key factor: Smaller autosomes and heteromorphic sex ..
  48. ncbi Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I
    Kirsten P Rabitsch
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Dev Cell 4:535-48. 2003
    Halving of the chromosome number during meiosis I depends on the segregation of maternal and paternal centromeres. This process relies on the attachment of sister centromeres to microtubules emanating from the same spindle pole...
  49. pmc Polo-like kinase Cdc5 drives exit from pachytene during budding yeast meiosis
    Anuradha Sourirajan
    Laboratory of Biochemistry and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    Genes Dev 22:2627-32. 2008
    In budding yeast, exit from the pachytene stage of meiosis requires the mid-meiosis transcription factor Ndt80, which promotes expression of approximately 200 genes...
  50. pmc Condensins regulate meiotic DNA break distribution, thus crossover frequency, by controlling chromosome structure
    David G Mets
    Howard Hughes Medical Institute, University of California Berkeley, Berkeley, CA 94720 3204, USA
    Cell 139:73-86. 2009
    ....
  51. pmc Variants of the protein PRDM9 differentially regulate a set of human meiotic recombination hotspots highly active in African populations
    Ingrid L Berg
    Department of Genetics, University of Leicester, Leicester LE1 7RH, UK
    Proc Natl Acad Sci U S A 108:12378-83. 2011
    ..This differential activation points to complex interactions between the zinc-finger array and hotspots and identifies features of the array that might be important in controlling hotspot activity...
  52. pmc Robust crossover assurance and regulated interhomolog access maintain meiotic crossover number
    Simona Rosu
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Science 334:1286-9. 2011
    ..By monitoring repair events at a defined double-strand break (DSB) site during Caenorhabditis elegans meiosis, we reveal mechanisms that ensure formation of the obligate CO while limiting CO number...
  53. ncbi Pathways to meiotic recombination in Arabidopsis thaliana
    Kim Osman
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK
    New Phytol 190:523-44. 2011
    b>Meiosis is a central feature of sexual reproduction. Studies in plants have made and continue to make an important contribution to fundamental research aimed at the understanding of this complex process...
  54. pmc Stage-specific expression profiling of Drosophila spermatogenesis suggests that meiotic sex chromosome inactivation drives genomic relocation of testis-expressed genes
    Maria D Vibranovski
    Department of Ecology, The University of Chicago, Chicago, Illinois, United States of America
    PLoS Genet 5:e1000731. 2009
    ..The inactivation of X-linked genes during male meiosis (i.e...
  55. ncbi Mechanism and control of meiotic recombination initiation
    S Keeney
    Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, and Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Curr Top Dev Biol 52:1-53. 2001
    Homologous recombination is essential during meiosis in most sexually reproducing organisms...
  56. ncbi The SUN rises on meiotic chromosome dynamics
    Yasushi Hiraoka
    Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamadaoka, Suita 565 0871, Japan
    Dev Cell 17:598-605. 2009
    ..During meiotic prophase, the apparent role of these SUN-domain proteins, together with their partner KASH-domain proteins, is to connect chromosomes through the intact nuclear envelope to force-generating mechanisms in the cytoplasm...
  57. ncbi Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11
    F Baudat
    Cell Biology Program Memorial Sloan Kettering Cancer Center New York, NY 10021, USA
    Mol Cell 6:989-98. 2000
    ..We now report that disruption of mouse Spo11 leads to severe gonadal abnormalities from defective meiosis. Spermatocytes suffer apoptotic death during early prophase; oocytes reach the diplotene/dictyate stage in nearly ..
  58. pmc Temporally and biochemically distinct activities of Exo1 during meiosis: double-strand break resection and resolution of double Holliday junctions
    Kseniya Zakharyevich
    Howard Hughes Medical Institute, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA
    Mol Cell 40:1001-15. 2010
    The Rad2/XPG family nuclease, Exo1, functions in a variety of DNA repair pathways. During meiosis, Exo1 promotes crossover recombination and thereby facilitates chromosome segregation at the first division...
  59. ncbi The centromere paradox: stable inheritance with rapidly evolving DNA
    S Henikoff
    Howard Hughes Medical Institute Research Laboratories, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA
    Science 293:1098-102. 2001
    Every eukaryotic chromosome has a centromere, the locus responsible for poleward movement at mitosis and meiosis. Although conventional loci are specified by their DNA sequences, current evidence favors a chromatin-based inheritance ..
  60. ncbi A mouse speciation gene encodes a meiotic histone H3 methyltransferase
    Ondrej Mihola
    Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague, Czech Republic
    Science 323:373-5. 2009
    ..These defects, seen also in Prdm9-null mutants, are rescued by the Prdm9 transgene. Identification of a vertebrate hybrid sterility gene reveals a role for epigenetics in speciation and opens a window to a hybrid sterility gene network...
  61. pmc Stra8 and its inducer, retinoic acid, regulate meiotic initiation in both spermatogenesis and oogenesis in mice
    Ericka L Anderson
    Howard Hughes Medical Institute, Whitehead Institute, and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 105:14976-80. 2008
    In eukaryotes, diploid cells give rise to haploid cells via meiosis, a program of two cell divisions preceded by one round of DNA replication...
  62. pmc Frequent and efficient use of the sister chromatid for DNA double-strand break repair during budding yeast meiosis
    Tamara Goldfarb
    Laboratory of Biochemistry and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America
    PLoS Biol 8:e1000520. 2010
    ..homologous chromosomes of different parental origin (homologs) is necessary for their accurate segregation during meiosis. It has been suggested that meiotic inter-homolog recombination is promoted by a barrier to inter-sister-..
  63. pmc Caenorhabditis elegans HIM-18/SLX-4 interacts with SLX-1 and XPF-1 and maintains genomic integrity in the germline by processing recombination intermediates
    Takamune T Saito
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Genet 5:e1000735. 2009
    ..A reduction in crossover recombination frequencies-accompanied by an increase in HR intermediates during meiosis, germ cell apoptosis, unstable bivalent attachments, and subsequent chromosome nondisjunction-support a role for ..
  64. ncbi Derivation of oocytes from mouse embryonic stem cells
    Karin Hübner
    Germline Development Group, Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, 382 West Street Road, Kennett Square, PA 19348, USA
    Science 300:1251-6. 2003
    ..Here we show that mouse embryonic stem cells in culture can develop into oogonia that enter meiosis, recruit adjacent cells to form follicle-like structures, and later develop into blastocysts...
  65. pmc MutS homolog 4 localization to meiotic chromosomes is required for chromosome pairing during meiosis in male and female mice
    B Kneitz
    Department of Cell Biology, Albert Einstein College of Medicine, The Bronx, New York 10461 USA
    Genes Dev 14:1085-97. 2000
    ..In this report we show that MSH4 has an essential role in the control of male and female meiosis. We demonstrate that MSH4 is present in the nuclei of spermatocytes early in prophase I and that it forms ..
  66. pmc Meiotic chromosome pairing is promoted by telomere-led chromosome movements independent of bouquet formation
    Chih Ying Lee
    Program in Cell Cycle and Cancer Biology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, United States of America
    PLoS Genet 8:e1002730. 2012
    ....
  67. ncbi The COMPASS subunit Spp1 links histone methylation to initiation of meiotic recombination
    Laurent Acquaviva
    Marseille Cancer Research Center, U1068 Inserm, UMR7258 CNRS, Aix Marseille University, Institut Paoli Calmettes, Marseille 13009, France
    Science 339:215-8. 2013
    During meiosis, combinatorial associations of genetic traits arise from homologous recombination between parental chromosomes...
  68. ncbi Evidence that meiotic sex chromosome inactivation is essential for male fertility
    Helene Royo
    Department of Stem Cell Research and Developmental Genetics, MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
    Curr Biol 20:2117-23. 2010
    The mammalian X and Y chromosomes share little homology and are largely unsynapsed during normal meiosis. This asynapsis triggers inactivation of X- and Y-linked genes, or meiotic sex chromosome inactivation (MSCI)...
  69. ncbi Homologous pairing and the role of pairing centers in meiosis
    Jui He Tsai
    Department of Biochemistry, Cellular, and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA
    J Cell Sci 124:1955-63. 2011
    Homologous pairing establishes the foundation for accurate reductional segregation during meiosis I in sexual organisms...
  70. ncbi An evolutionary view of human recombination
    Graham Coop
    Department of Human Genetics, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA
    Nat Rev Genet 8:23-34. 2007
    Recombination has essential functions in mammalian meiosis, which impose several constraints on the recombination process...
  71. ncbi Shugoshin collaborates with protein phosphatase 2A to protect cohesin
    Tomoya S Kitajima
    Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    Nature 441:46-52. 2006
    ..complex called cohesin, is crucial--particularly at centromeres--for proper chromosome segregation in mitosis and meiosis. In animal mitotic cells, phosphorylation of cohesin promotes its dissociation from chromosomes, but centromeric ..
  72. ncbi Silencing of unsynapsed meiotic chromosomes in the mouse
    James M A Turner
    Division of Stem Cell Research and Developmental Genetics, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Nat Genet 37:41-7. 2005
    In Neurospora, DNA unpaired in meiosis both is silenced and induces silencing of all DNA homologous to it...
  73. pmc Histone H3 lysine 4 trimethylation marks meiotic recombination initiation sites
    Valerie Borde
    Institut Curie Centre de Recherche, UMR7147 CNRS, Universite Pierre et Marie Curie, Paris, France
    EMBO J 28:99-111. 2009
    ..cerevisiae, we examined the genome-wide localization of histone H3 lysine 4 trimethylation (H3K4me3) along meiosis and its relationship to gene expression and position of the programmed double-strand breaks (DSBs) that initiate ..
  74. ncbi Mili, a mammalian member of piwi family gene, is essential for spermatogenesis
    Satomi Kuramochi-Miyagawa
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita Shi, Osaka 565 0871, Japan
    Development 131:839-49. 2004
    ..Spermatogenesis in the MILI-null mice was blocked completely at the early prophase of the first meiosis, from the zygotene to early pachytene, and the mice were sterile...
  75. pmc Chromosomal gene movements reflect the recent origin and biology of therian sex chromosomes
    Lukasz Potrzebowski
    Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland, 2 INSERM U625, IFR 140, Universite Rennes I, Campus de Beaulieu, Rennes, France
    PLoS Biol 6:e80. 2008
    ....
  76. ncbi A histone H3 methyltransferase controls epigenetic events required for meiotic prophase
    Katsuhiko Hayashi
    Department of Molecular Embryology, Research Institute, Osaka Medical Center for Maternal and Child Health, Murodo cho 840, Izumi, Osaka 594 1101, Japan
    Nature 438:374-8. 2005
    Epigenetic modifications of histones regulate gene expression and chromatin structure. Here we show that Meisetz (meiosis-induced factor containing a PR/SET domain and zinc-finger motif) is a histone methyltransferase that is important ..
  77. pmc The fission yeast RNA binding protein Mmi1 regulates meiotic genes by controlling intron specific splicing and polyadenylation coupled RNA turnover
    Huei Mei Chen
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, United States of America
    PLoS ONE 6:e26804. 2011
    ..In addition, binding of Mmi1 can affect the efficiency of 3' cleavage. Inactivation of Mmi1 in meiosis allows meiotic expression, through splicing and RNA stabilization, of at least 29 target genes, which are ..
  78. pmc A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing
    Kei Ichiro Ishiguro
    Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Tokyo 113 0032, Japan
    EMBO Rep 12:267-75. 2011
    ..Further, we show that the removal of cohesins and synaptonemal complex during late meiotic prophase is promoted by Polo-like kinase 1, which is similar to the mitotic prophase pathway...
  79. pmc Temporal characterization of homology-independent centromere coupling in meiotic prophase
    David Obeso
    Cell Cycle and Cancer Biology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, United States of America
    PLoS ONE 5:e10336. 2010
    ..that centromeres would pair with non-homologous partners during a process intended to align homologous chromosomes, yet the conservation of this phenomenon across a wide range of species suggests it may play an important role in meiosis.
  80. pmc Regulation of APC/C activators in mitosis and meiosis
    Jillian A Pesin
    Whitehead Institute and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Annu Rev Cell Dev Biol 24:475-99. 2008
    ..During the specialized divisions of meiosis, the activity of the APC/C must be modified to achieve proper chromosome segregation...
  81. ncbi Mammalian recombination hot spots: properties, control and evolution
    Kenneth Paigen
    The Jackson Laboratory, Bar Harbor, Maine 04609, USA
    Nat Rev Genet 11:221-33. 2010
    ..phenomenon in humans and mice: from describing the properties of recombination hot spots in male and female meiosis to the recombination landscape along chromosomes...
  82. ncbi RTEL-1 enforces meiotic crossover interference and homeostasis
    Jillian L Youds
    DNA Damage Response Laboratory, London Research Institute, Cancer Research UK, Clare Hall, South Mimms, EN6 3LD, UK
    Science 327:1254-8. 2010
    ..Two distinct classes of meiotic COs are increased in rtel-1 mutants, and COI and homeostasis are compromised. We propose that RTEL-1 implements the second level of CO control by promoting noncrossovers...
  83. pmc Distinct histone modifications define initiation and repair of meiotic recombination in the mouse
    Jerome Buard
    Institut de Genetique Humaine, CNRS UPR 1142, Montpellier, France
    EMBO J 28:2616-24. 2009
    ..Altogether, the chromatin signatures of the Psmb9 and Hlx1 hotspots provide a basis for understanding the distribution of meiotic recombination...
  84. pmc Distribution of meiotic recombination events: talking to your neighbors
    Enrique Martinez-Perez
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK
    Curr Opin Genet Dev 19:105-12. 2009
    Accurate chromosome segregation during meiosis is essential for a species' survival...
  85. ncbi Chromosome sites play dual roles to establish homologous synapsis during meiosis in C. elegans
    Amy J MacQueen
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 123:1037-50. 2005
    ..of pairing centers (PCs), cis-acting sites required for accurate segregation of homologous chromosomes during meiosis in C. elegans. We find that these sites play two distinct roles that contribute to proper segregation...
  86. pmc High-resolution mapping of meiotic crossovers and non-crossovers in yeast
    Eugenio Mancera
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nature 454:479-85. 2008
    ..Up to 1% of the genome of each meiotic product is subject to gene conversion in a single meiosis, with detectable bias towards GC nucleotides...
  87. ncbi DNA double strand break repair, chromosome synapsis and transcriptional silencing in meiosis
    Akiko Inagaki
    Department of Reproduction and Development, Erasmus MC University Medical Center, Rotterdam, The Netherlands
    Epigenetics 5:255-66. 2010
    ..Herein, we review meiosis-specific aspects of DSB repair in relation to homology recognition and meiotic silencing of heterologous regions...
  88. pmc Genome-wide crossover distribution in Arabidopsis thaliana meiosis reveals sex-specific patterns along chromosomes
    Laurène Giraut
    Institut Jean Pierre Bourgin, UMR1318 INRA AgroParisTech, INRA Centre de Versailles Grignon, Versailles, France
    PLoS Genet 7:e1002354. 2011
    ..genome-wide characterization of the rate and localization of COs in Arabidopsis thaliana, in male and female meiosis. We observed dramatic differences between male and female meiosis which included: (i) genetic map length; 575 cM ..
  89. pmc Dynein-dependent processive chromosome motions promote homologous pairing in C. elegans meiosis
    David J Wynne
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 196:47-64. 2012
    ..We find that chromosome movement is constrained before meiosis. Upon prophase onset, constraints are relaxed, and PCs initiate saltatory, processive, dynein-dependent motions ..
  90. pmc Dissociation of the Nuf2-Ndc80 complex releases centromeres from the spindle-pole body during meiotic prophase in fission yeast
    Haruhiko Asakawa
    Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
    Mol Biol Cell 16:2325-38. 2005
    ..The centromere protein Mis12 also dissociates during meiotic prophase; however, Mis6 remains throughout meiosis. When cells are induced to meiosis by inactivation of Pat1 kinase (a key negative regulator of meiosis), ..
  91. pmc Mouse HORMAD1 and HORMAD2, two conserved meiotic chromosomal proteins, are depleted from synapsed chromosome axes with the help of TRIP13 AAA-ATPase
    Lukasz Wojtasz
    Institute of Physiological Chemistry, Technische Universitat Dresden, Dresden, Germany
    PLoS Genet 5:e1000702. 2009
    ..We examined the behavior of two previously uncharacterized meiosis-specific mouse HORMA-domain proteins--HORMAD1 and HORMAD2--in wild-type mice and in mutants defective in DSB ..
  92. ncbi Chromosomal passengers: conducting cell division
    Sandrine Ruchaud
    Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, Swann Building, King s Buildings, Mayfield Road, Edinburgh, EH9 3JR, UK
    Nat Rev Mol Cell Biol 8:798-812. 2007
    Mitosis and meiosis are remarkable processes during which cells undergo profound changes in their structure and physiology...
  93. pmc The impressionistic landscape of meiotic recombination
    Michael Lichten
    Laboratory of Biochemistry and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Cell 147:267-70. 2011
    ..These studies offer new insights into the relationships between recombination, chromosome structure, and genome evolution...
  94. pmc MDC1 directs chromosome-wide silencing of the sex chromosomes in male germ cells
    Yosuke Ichijima
    Division of Reproductive Sciences, Perinatal Institute, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    Genes Dev 25:959-71. 2011
    ..These results establish that the DDR pathway centered on MDC1 triggers epigenetic silencing of sex chromosomes in germ cells...
  95. pmc Mouse PRDM9 DNA-binding specificity determines sites of histone H3 lysine 4 trimethylation for initiation of meiotic recombination
    Corinne Grey
    Institut de Genetique Humaine, CNRS UPR, Montpellier, France
    PLoS Biol 9:e1001176. 2011
    ..homologous chromosomes (also called crossovers, COs) that are essential for proper chromosome segregation during meiosis and are a major source of genome diversity by generating new allele combinations...
  96. pmc A single gene causes both male sterility and segregation distortion in Drosophila hybrids
    Nitin Phadnis
    Department of Biology, University of Rochester, Rochester, NY 14627 0211, USA
    Science 323:376-9. 2009
    ..This segregation distorter gene is essential for hybrid sterility, a strong reproductive barrier between these young taxa. Our results suggest that genetic conflict may be an important evolutionary force in speciation...
  97. pmc Protection of repetitive DNA borders from self-induced meiotic instability
    Gerben Vader
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 477:115-9. 2011
    ..Repetitive sequences are especially at risk during meiosis, when numerous programmed DSBs are introduced into the genome to initiate meiotic recombination...
  98. pmc Meiotic DNA double-strand break repair requires two nucleases, MRN and Ctp1, to produce a single size class of Rec12 (Spo11)-oligonucleotide complexes
    Neta Milman
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, A1 162, Seattle, WA 98109, USA
    Mol Cell Biol 29:5998-6005. 2009
    Programmed DNA double-strand breaks (DSBs) in meiosis are formed by Spo11 (Rec12 in fission yeast), a topoisomerase II-like protein, which becomes covalently attached to DNA 5' ends...
  99. pmc Critical roles for Dicer in the female germline
    Elizabeth P Murchison
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, Cold Spring Harbor, New York 11724, USA
    Genes Dev 21:682-93. 2007
    ..Probing the consequences of tissue-restricted Dicer loss in mice indicates a critical role for Dicer during meiosis in the female germline...
  100. ncbi Phosphorylation of histone H3: a balancing act between chromosome condensation and transcriptional activation
    Scott J Nowak
    Department of Biology, The Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA
    Trends Genet 20:214-20. 2004
    ..tail of histone H3 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, this modification is important during interphase because it enables the transcription of an ..
  101. pmc Altered heterochromatin binding by a hybrid sterility protein in Drosophila sibling species
    Joshua J Bayes
    Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98185, USA
    Science 326:1538-41. 2009
    ..These results reveal that rapid heterochromatin evolution affects the onset of hybrid sterility...

Research Grants73

  1. Genome-wide recombination as a risk factor for nondisjunction
    NATASHA D HOLLIS; Fiscal Year: 2010
    ..risk factors are identified, targeted treatment can be considered to assure proper chromosome segregation during meiosis. To accomplish the specific aims of the proposal, we will perform genome-wide SNP genotyping on probands and ..
  2. Mechanisms of Sex Determination in Zebrafish
    JOHN HARVEY POSTLETHWAIT; Fiscal Year: 2013
    ..test additional components of the hypothesis by learning whether blocking the retinoic acid signal for entry into meiosis can cause sex reversal (Aim 3a) and whether primordial germ cell number controls sex development in zebrafish and ..
  3. Functional Analysis of the Dual Specificity Kinase NEK1 in Mammalian Meiosis
    JOANNA KIM HOLLOWAY; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): During meiosis, homologous chromosomes seek each other out and are then tethered together by the synaptonemal complex (SC) and sister chromatid cohesion, without which homologous recombination and ..
  4. Analysis of the role of the VRK1 protein kinase in mammalian fertility
    Paula Traktman; Fiscal Year: 2011
    ..We therefore hypothesize that, within oocytes, VRK1 may be most critical for the resumption and completion of meiosis. Our preliminary data provides the first evidence that VRK1 plays a key role in mammalian gametogenesis...
  5. Chromosomal Control of Segregation of C. Elegans
    Abby F Dernburg; Fiscal Year: 2013
    ..doubling their chromosome number with each successive generation, all sexually reproducing organisms must undergo meiosis. This special cell division process gives rise to haploid gametes such as sperm, eggs, and pollen, which contain ..
  6. Functional Analysis of the Dual Specificity Kinase NEK1 in Mammalian Meiosis
    JOANNA KIM HOLLOWAY; Fiscal Year: 2013
    Project Summary During meiosis, homologous chromosomes seek each other out and are then tethered together by the synaptonemal complex (SC) and sister chromatid cohesion, without which homologous recombination and meiotic division cannot ..
  7. Model of the Human Testis for Reproductive Toxicology
    PAUL JACOB TUREK; Fiscal Year: 2011
    ..we have been able to modulate human germ-cell formation and developmental progression, to promote later stages of meiosis and development of haploid gametes...
  8. The role of satellite rasiRNA in the Segregation Distorter system of Drosophila
    AMANDA MARIE LARRACUENTE; Fiscal Year: 2013
    ..Preliminary results described in this proposal show Rsp rasiRNA expression in the testis after meiosis. This proposal will test the hypothesis that SD interferes with Rsp rasiRNA localization or expression by looking ..
  9. AAA ATPases linking mitochondria with microtubule processing in flies and yeast
    Karen G Hales; Fiscal Year: 2012
    ..After meiosis, all mitochondria in a Drosophila spermatid normally fuse into two giant mitochondrial derivatives that interwrap ..
  10. Centromere Structure and Function in Candida albicans
    Judith G Berman; Fiscal Year: 2012
    ..C. albicans does not undergo meiosis;genetic diversity arises through somatic mitotic events such as chromosome non-disjunction...
  11. Assembly and Maintenance of Centromeres in Filamentous Fungi
    Michael Freitag; Fiscal Year: 2013
    ..nucleosomes with heterochromatic histone modifications, and that incorporation of CenH3 during meiosis is controlled by a novel mechanism mediated via CenH3 mRNA...
  12. ANALYSIS OF MEIOTIC CHROMOSOME SYNAPSIS IN YEAST
    Nancy M Hollingsworth; Fiscal Year: 2013
    ..reduction in chromosome number is carried out by a specialized, evolutionarily conserved, cell division called meiosis. In humans, failures in meiosis result in infertility and birth defects such as Trisomy 21 or Down Syndrome...
  13. The Role of Cks Proteins in Mammalian Meiosis
    Charles H Spruck; Fiscal Year: 2010
    ..We functionally inactivated the ortholog Cks2 in mice and found that it plays an essential role in mammalian meiosis. CKS2-/- male and female mice are sterile due to an arrest of spermatocytes and oocytes in metaphase of meiosis I ..
  14. PTIP as an epigenetic regulator in male meiosis
    Guo Zhang Zhu; Fiscal Year: 2013
    ..that PTIP (PAX transcription activation domain interacting protein) is an essential epigenetic regulator in male meiosis. PTIP has been lately identified as part of a histone H3 lysine 4 (H3K4) methyltransferase complex and is ..
  15. Checkpoints and double strand breaks in S. pombe meiosis
    Susan L Forsburg; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Genome instability resulting in chromosome mis-segregation during meiosis accounts for as many as 50% of human miscarriages and contributes to birth defects such as Down Syndrome...
  16. Laser Spinning Disc Confocal System for live-cell and live-organism microscopy
    Martha C Soto; Fiscal Year: 2012
    ..elegans as a model system to study human ciliopathies, or diseases of cilia. Drosophila studies of meiosis in the McKim lab have identified molecules required for proper fertility through the development of germ cells, ..
  17. EGF RECEPTOR MEDIATED SIGNALING IN DROSOPHILA
    GERTRUD M SCHUPBACH; Fiscal Year: 2013
    ..Problems in DNA repair during meiosis as well as other nuclear defects caused by retrotransposons, activate a meiotic checkpoint that controls ..
  18. CELL CYCLE CONTROL IN EARLY DROSOPHILA DEVELOPMENT
    Terry L Orr-Weaver; Fiscal Year: 2013
    ..The prerequisites for this are the completion of meiosis, the modified cell cycle used to produce haploid eggs and sperm, and the activation of DNA replication and ..
  19. Meiosis, SUMOylation and the ZIP3 Protein: Parallel Studies in Mouse and Yeast.
    Neil Hunter; Fiscal Year: 2012
    Crossing-over is essential for accurate chromosome segregation during meiosis. In Saccharomyces cerevisiae, crossing-over is specifically promoted by ScZip3, a putative E3-ligase for SUMO...
  20. Mechanics and regulation of chromosome dynamics in meiotic prophase
    MICHAEL EDWARD DRESSER; Fiscal Year: 2012
    Human genetic disease frequently arises from the failure of meiosis to faithfully haploidize the genome, instead giving rise to aneuploidy...
  21. Environmental Endocrine Disruptors and Human Sperm Chromosomal Abnormalities
    Melissa Perry; Fiscal Year: 2012
    ..About 40 percent of cases of human infertility are due to male factors. Errors in chromosome segregation during meiosis result in structural aberrations and imbalances in chromosome number known as aneuploidy...
  22. Molecular genetics of egg activation and pronuclear function in Drosophila
    MARIANA FEDERICA WOLFNER; Fiscal Year: 2010
    ..For example, activation is needed to block polyspermy, resume meiosis and start embryo mitosis...
  23. Leukemia Inhibitory Factor As a Mediator of Primate Ovulation &Oocyte Maturation
    Jon D Hennebold; Fiscal Year: 2013
    ..for ovulation and formation of the corpus luteum (assessed in Aim 1);as well as for C-OE, reinitiation of oocyte meiosis, fertilization, and early embryonic development (assessed in Aim 2)...
  24. CONTROL OF MEIOSIS AND GERMLINE PROLIFERATION
    DAVID IRWIN GREENSTEIN; Fiscal Year: 2012
    ..In many species, intercellular signaling plays a pivotal role in coordinating meiosis and fertilization: developing oocytes arrest at diakinesis for prolonged periods and resume meiosis (meiotic ..
  25. Role of Fetal Steroids in Formation and Activation of Bovine Ovarian Follicles
    Joanne E Fortune; Fiscal Year: 2010
    ..formed primordial follicles and that they do this by inhibiting or regulating the progression of prophase I of meiosis (bovine primordial follicles form before the oocyte has reached meiotic arrest in the diplotene stage of prophase ..
  26. Design of a high-throughput screen for chemicals that cause meiotic aneuploidy
    Patrick Allard; Fiscal Year: 2011
    ..errors in the segregation of chromosomes during the two highly regulated programs of cell division, mitosis and meiosis. The consequences of aneuploidies of meiotic origins are devastating as they contribute to 35% of clinically ..
  27. The Synapsis Checkpoint in C. elegans Meiosis
    Needhi Bhalla; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Meiosis generates haploid gametes, such as sperm and eggs, from a diploid cell such that a diploid genome is restored upon fertilization...
  28. Genetics of Meiosis and Recombination in Mice
    John C Schimenti; Fiscal Year: 2013
    ..Errors in meiosis can lad to aneuploidy, chromosome aberrations, or gene mutations...
  29. 2011 Cold Spring Harbor Conference on Telomeres and Telomerase
    David J Stewart; Fiscal Year: 2010
    ..dynamic research area, representing a wide variety of research interests (cancer, aging, stem cells, cell cycle, meiosis, recombination, replication, etc...
  30. Epigenetic control of sex chromosome behavior in meiosis.
    JoAnne Engebrecht; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Meiosis is a special type of cell division that produces haploid gametes for sexual reproduction...
  31. Kinetics of Chromosome Synapsis During Meiosis
    Jennifer C Fung; Fiscal Year: 2013
    ..this research is to determine how chromosome synapsis functions to promote proper chromosome segregation during meiosis. Chromosome missegregation during meiosis is directly tied to human infertility and is also the leading known ..
  32. Developmental Regulation of the Cell Cycle in Drosophila
    LAURA ANNE LEE; Fiscal Year: 2013
    ..asun spermatocytes arrest during prophase of meiosis I. Strikingly, arrested spermatocytes contain free centrosomes that fail to stably associate with the nucleus...
  33. MOLECULAR BASIS OF MAMMALIAN SPERM MOTILITY
    Jerome F Strauss; Fiscal Year: 2010
    ..with a testis-specific serine threonine kinase, TSSK-2, and a chromosome-binding protein in the nucleus, meiosis induced gene 1 (MEIG1)...
  34. Role of Cyclin A's in mammalian gametogenesis
    Debra J Wolgemuth; Fiscal Year: 2013
    ..Our findings will enhance our understanding of the control of meiosis as it relates to male infertility, possibly suggest new targets for contraception, and provide critical insight ..
  35. Regulation of Spermatocyte Transcription by Testis TAFS
    Margaret T Fuller; Fiscal Year: 2013
    ..understanding the mode of action of the meiotic arrest genes of Drosophila may illuminate mechanisms underlying meiosis I maturation arrest infertility in man...
  36. Control of Oocyte Maturation in C. elegans
    DAVID IRWIN GREENSTEIN; Fiscal Year: 2013
    ..provided by applicant): Our objective is to define the mechanisms by which intercellular signaling coordinates meiosis and fertilization in the nematode C. elegans...
  37. Regulation of RNA Surveillance
    MILES FROME WILKINSON; Fiscal Year: 2012
    ..its paralog, UPF3B, is present on the X chromosome, which is known to be transcriptionally inactivated during meiosis in male germ cells. In this proposal, the function of UPF3A in male germ cells and other cell types is addressed...
  38. Identification of DNA Elements Governing Chromatin Function in C elegans
    Jason D Lieb; Fiscal Year: 2011
    ..other essential processes, including recombination and the faithful segregation of the genome during mitosis and meiosis. The goal of this proposal is to identify discrete elements that regulate chromatin structure and function in the ..
  39. Germline Silencing of Unpaired Chromatin
    Eleanor M Maine; Fiscal Year: 2013
    ..or chromosomal regions that arise due to mutation or chromosomal rearrangement during either female or male meiosis. Meiotic silencing is hypothesized to function in several ways to promote fertility and gamete quality...
  40. Kinetochore Function and Cell Cycle Progression Revision
    Katsumi Kitagawa; Fiscal Year: 2012
    ..centromere DNA and associated proteins, is crucial for maintaining and segregating chromosomes during mitosis and meiosis. For these studies we will use the budding yeast Saccharomyces cerevisiae, as its process of mitotic division is ..
  41. Homolog orientation and segregation in acentrosomal meiosis
    Kim S McKim; Fiscal Year: 2013
    ..between the poles and the kinetochores facilitates bi-orientation of sisters (mitosis) or homologous chromosomes (meiosis I). In acentrosomal cells, novel mechanisms may be employed to bi-orient the homologs...
  42. Mechanisms of Nuclear and Cell Fusion in Yeast
    MARK DAVID ROSE; Fiscal Year: 2013
    ..a transcriptional activator of mating, Kar4p, is able to support a different regulatory program during entry into meiosis. Although numerous cell fusion events occur during development and disease, the mechanism of cell fusion is ..
  43. Ovarian-specific transcription networks regulated by the TFIID subunit TAF4b
    RICHARD NEIL FREIMAN; Fiscal Year: 2013
    ..primordial follicle attrition;2) elucidate the TAF4b regulation of chromosome synapsis, segregation and meiosis;and 3) determine the mechanistic basis of TAF4b-dependent regulation of oocyte quality...
  44. Biochemistry of lysine crotonylation pathway
    Yingming Zhao; Fiscal Year: 2013
    ..In male germinal cells immediately following meiosis, histone Kcr, but not histone Kac, is enriched on sex chromosomes and specifically marks testis-specific genes...
  45. Synaptonemal complex assembly and function in meiosis
    MONICA P COLAIACOVO; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Meiosis is a specialized cell division process that results in the formation of haploid gametes (i.e...
  46. Regulation of meiotic recombination and chromosome segregation in mammals
    KAREN MICHELE BERKOWITZ; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): DNA double strand break repair and crossover formation during meiosis are essential to ensure proper chromosome segregation...
  47. Characterization of the Synapis Checkpoint in C. elegans Meiosis
    Needhi Bhalla; Fiscal Year: 2010
    b>Meiosis generates haploid gametes from a diploid cell such that a diploid genome is restored upon fertilization...
  48. Genomic Stability Functions of SMC Proteins in Germ Cells
    Raymond C Chan; Fiscal Year: 2013
    ..repair process is the lack of experimental systems to specifically interrogate sister-chromatid recombination in meiosis, because there is frequent recombination occurring between homologous chromosomes...
  49. Age and molecular mechanisms contributing to aneuploidy in oocytes
    Richard M Schultz; Fiscal Year: 2013
    ..associated with increased maternal age are due to non-disjunction and meiotic errors that occur during meiosis. Remarkably, the underlying molecular mechanisms that lead to the age-associated increase in aneuploidy are ..
  50. Signaling pathways that mediate mammalian oocyte cortical mechanics
    Douglas N Robinson; Fiscal Year: 2013
    ..Defects in these processes during meiosis I or II can compromise egg quality and competence to form a healthy embryo, thus negatively impacting female ..
  51. Role of CDC14B in mouse oocyte maturation
    KAREN A SCHINDLER; Fiscal Year: 2010
    ..Haploid gametes, that are essential for sexual reproduction, are generated from diploid precursor cells via meiosis. In females, oocytes undergo 2 major meiosis-specific, cell-cycle transitions whose regulation is poorly ..
  52. Understanding the roles of histone demethylation in germline maintenance
    SARA ELIZABETH BEESE-SIMS; Fiscal Year: 2013
    ..where defects can result in cancers, and also plays a particularly prominent role in crossover formation during meiosis, where defects can result in a variety of birth abnormalities, infertility and miscarriages...
  53. The Control of Centriole Duplication and Degeneration
    Meng Fu Bryan Tsou; Fiscal Year: 2013
    ..During meiosis, the centrosome copy number is reduced (centriole degeneration) so that upon fertilization, the proper number can ..
  54. Regulation of cell division by mitotic kinases
    MICHAEL ALTERMAN LAMPSON; Fiscal Year: 2012
    ..is strongly associated with human cancer in mitotic divisions and pregnancy loss and developmental defects in meiosis. Much of the complex dynamics of cell division is controlled by a few key regulatory kinases...
  55. Evolutionary and functional diversification of chromatin proteins
    MIA TAUNA LEVINE; Fiscal Year: 2013
    ..evolution reflects recurrent innovation to suppress the fitness costs of selfih telomeres that "cheat" female meiosis via non-Mendelian segregation. This hypothesis is tested by transgenically introducing into D...
  56. A Molecular View of Chromosome Recombination &Segregation in Eukaryotic Meiosis
    KEVIN DANIEL CORBETT; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): PROJECT SUMMARY Meiosis is a specialized cell division program that gives rise to gametes in sexually reproducing organisms...
  57. Developing Male Contraceptives by Targeting ANT4
    Naohiro Terada; Fiscal Year: 2013
    ..Ant4 expression is particularly high during male meiosis and is essential for progression of male meiosis...
  58. Molecular Mechanisms of Meiotic Maturation in Drosophila
    Daniela Drummond-Barbosa; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Meiosis is a fundamental process that is coupled to gamete development. Oocytes arrest in prophase of the first meiotic division to allow for growth...
  59. The Novel Role of Branch Migration Protein Rad54 in Maintaining Genome Stability
    MATTHEW JOHN ROSSI; Fiscal Year: 2011
    ..double-strand DNA breaks and exchanging genetic material between homologous chromosomes in cells undergoing meiosis. Our goal is to understand the mechanisms of homologous recombination in humans...
  60. Regulation of Sister-Chromatid Cohesion in Drosophila Meiosis
    SHARON ELIZABETH BICKEL; Fiscal Year: 2013
    ..In humans, female meiosis is especially error-prone and the incidence of chromosome segregation errors increases dramatically as women age...
  61. Nikon A1RSI Confocal Microscope
    Sailen Barik; Fiscal Year: 2012
    ..of Trypanosome telomere complex;Endothelial cell drug delivery;Chromosome structure and recombination during meiosis;Circadian clock location and function;Toxoplasma-host mitochondria interaction;Regulation of innate immunity by ..
  62. 2012 Meiosis Gordon Research Conference and Gordon Research Seminar
    Nancy M Hollingsworth; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Partial support is requested for an international meeting on Meiosis as part of the Gordon Research Conferences (GRC) to be held at Colby-Sawyer College in New London, NH on June 3-8, 2012...
  63. MEIOTIC CELL CYCLE REGULATION IN REPRODUCTION
    Stuart Ravnik; Fiscal Year: 2002
    ..Germ cell development and meiosis require a highly orchestrated series of unique cell cycle events...
  64. Novel Informatics for Highly Reliable Multi-Locus Allele Calling for Embryo Scree
    Matthew Rabinowitz; Fiscal Year: 2009
    ..technique makes use of genetic data of the mother and the father, together with the knowledge of the mechanism of meiosis and noisy measurements of the embryonic DNA, in order to determine which segments of parent chromosomes ..
  65. MEIOTIC CHROMOSOME SEGREGATION IN YEAST
    Dean Dawson; Fiscal Year: 1992
    In eucaryotic organisms, haploid gametes are generated from diploid cells during meiosis. In the first meiotic division, homologous chromosomes migrate to opposite poles of the cell (disjoin)...
  66. CHROMOSOME PAIRING AND NONDISJUNCTION IN HUMAN MEIOSIS
    Edith Cheng; Fiscal Year: 2000
    ..Multicolor FISH analysis will permit the detection of heterologous associations in early meiosis which could be indicative of the hypothesized "searching" or "trial and error" process preceding pairing...
  67. SAGE Analysis of Murine Oocyte Maturation
    CHARLOTTE FARIN; Fiscal Year: 2004
    ..Identification of mechanisms for the resumption of meiosis in oocytes will facilitate the development of strategies to improve the effectiveness of assisted reproduction ..
  68. PROTEIN PHOSPHATASES AND OOCYTE MEIOSIS
    Gary Smith; Fiscal Year: 2002
    ..intracellular mechanisms governing development of germinal vesicle breakdown (GVB) competence and resumption of meiosis are incomplete...
  69. 2004 & 2006 Meiosis Gordon Conference
    DOUGLAS BISHOP; Fiscal Year: 2005
    The Meiosis Gordon Conference is held bi-annually at Colby-Sawyer College in New London, New Hampshire. The 2004 meeting is scheduled for June 13-18. The 2006 meeting has yet to be scheduled but is likely to be held in early June...
  70. HU-IME1 CHARACTERIZATION AND MALE INFERTILITY
    Wael Salameh; Fiscal Year: 1999
    ..b>Meiosis occurs only in the gonads...
  71. Comparative and functional proteomics of germline chromatin in C. elegans
    KENNETH HILLERS; Fiscal Year: 2009
    ..in diploid eukaryotes involves formation of haploid gametes through a specialized cell division known as meiosis. Fusion of two haploid gametes at fertilization recreates diploidy in the next generation...
  72. MEIOSIS GORDON CONFERENCE
    Anne Villeneuve; Fiscal Year: 2002
    The Meiosis Gordon Conference will be held from June 16-21, 2002 at Colby-Sawyer College in New London, New Hampshire...
  73. Segregation of error-prone chromosomes in meiosis
    Dean S Dawson; Fiscal Year: 2010
    ..chromosomes greatly increases the probability that they will segregate properly (away from each other) at meiosis I...