anaphase

Summary

Summary: The phase of cell nucleus division following METAPHASE, in which the CHROMATIDS separate and migrate to opposite poles of the spindle.

Top Publications

  1. ncbi Cohesins: chromosomal proteins that prevent premature separation of sister chromatids
    C Michaelis
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 91:35-45. 1997
  2. ncbi Un ménage à quatre: the molecular biology of chromosome segregation in meiosis
    Mark Petronczki
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, 1030 Vienna, Austria
    Cell 112:423-40. 2003
  3. ncbi Mitotic kinases as regulators of cell division and its checkpoints
    E A Nigg
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Nat Rev Mol Cell Biol 2:21-32. 2001
  4. pmc Aurora A and Aurora B jointly coordinate chromosome segregation and anaphase microtubule dynamics
    Nadia Hegarat
    Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, England, UK
    J Cell Biol 195:1103-13. 2011
  5. pmc BLM is required for faithful chromosome segregation and its localization defines a class of ultrafine anaphase bridges
    Kok Lung Chan
    Cancer Research UK Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK
    EMBO J 26:3397-409. 2007
  6. ncbi Identification of a vertebrate sister-chromatid separation inhibitor involved in transformation and tumorigenesis
    H Zou
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Science 285:418-22. 1999
  7. ncbi TRF2 protects human telomeres from end-to-end fusions
    B van Steensel
    The Rockefeller University, New York, New York 10021, USA
    Cell 92:401-13. 1998
  8. ncbi An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast
    R Ciosk
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 93:1067-76. 1998
  9. ncbi Degradation of cyclin B is required for the onset of anaphase in Mammalian cells
    Donald C Chang
    Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Biol Chem 278:37865-73. 2003
  10. pmc Cell cycle-regulated cortical dynein/dynactin promotes symmetric cell division by differential pole motion in anaphase
    Elizabeth S Collins
    Biology Department, University of Massachusetts, Amherst, MA 01003, USA
    Mol Biol Cell 23:3380-90. 2012

Detail Information

Publications364 found, 100 shown here

  1. ncbi Cohesins: chromosomal proteins that prevent premature separation of sister chromatids
    C Michaelis
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 91:35-45. 1997
    ..microtubules, and loss of this cohesion is responsible for the subsequent separation of sister chromatids during anaphase. We describe three chromosmal proteins that prevent premature separation of sister chromatids in yeast...
  2. ncbi Un ménage à quatre: the molecular biology of chromosome segregation in meiosis
    Mark Petronczki
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, 1030 Vienna, Austria
    Cell 112:423-40. 2003
    ....
  3. ncbi Mitotic kinases as regulators of cell division and its checkpoints
    E A Nigg
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Nat Rev Mol Cell Biol 2:21-32. 2001
    ..Here, I give an overview of the many mitotic kinases that regulate cell division and the fidelity of chromosome transmission...
  4. pmc Aurora A and Aurora B jointly coordinate chromosome segregation and anaphase microtubule dynamics
    Nadia Hegarat
    Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, England, UK
    J Cell Biol 195:1103-13. 2011
    ..Conversely, cells lacking AurA and AurB activity exit mitosis without anaphase, forming polyploid daughter cells with a single nucleus...
  5. pmc BLM is required for faithful chromosome segregation and its localization defines a class of ultrafine anaphase bridges
    Kok Lung Chan
    Cancer Research UK Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK
    EMBO J 26:3397-409. 2007
    ..We show that BLM-defective cells display a higher frequency of anaphase bridges and lagging chromatin than do isogenic corrected derivatives that eptopically express the BLM protein...
  6. ncbi Identification of a vertebrate sister-chromatid separation inhibitor involved in transformation and tumorigenesis
    H Zou
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Science 285:418-22. 1999
    ..bound to a vertebrate homolog of yeast separins Esp1p and Cut1p and was degraded by proteolysis mediated by an anaphase-promoting complex in a manner dependent on a destruction motif...
  7. ncbi TRF2 protects human telomeres from end-to-end fusions
    B van Steensel
    The Rockefeller University, New York, New York 10021, USA
    Cell 92:401-13. 1998
    ..A dominant negative allele of TRF2 induced end-to-end chromosome fusions detectable in metaphase and anaphase cells...
  8. ncbi An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast
    R Ciosk
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 93:1067-76. 1998
    ..Loss of cohesion at the metaphase to anaphase transition is accompanied by Scc1p's dissociation from chromatids, which depends on proteolysis of Pds1p mediated ..
  9. ncbi Degradation of cyclin B is required for the onset of anaphase in Mammalian cells
    Donald C Chang
    Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Biol Chem 278:37865-73. 2003
    Recently, it has been shown that cyclin B1 was degraded mainly before the onset of anaphase in mammalian cells. When a nondegradable form of cyclin B1 was introduced into cells, the metaphase-anaphase transition was blocked...
  10. pmc Cell cycle-regulated cortical dynein/dynactin promotes symmetric cell division by differential pole motion in anaphase
    Elizabeth S Collins
    Biology Department, University of Massachusetts, Amherst, MA 01003, USA
    Mol Biol Cell 23:3380-90. 2012
    ..At anaphase onset, we observed a transient increase in cortical dynein, followed by a reduction in telophase...
  11. ncbi New insights into the formation and resolution of ultra-fine anaphase bridges
    Kok Lung Chan
    Department of Biochemistry, University of Oxford, Oxford, UK
    Semin Cell Dev Biol 22:906-12. 2011
    ..hitherto considered to be dedicated to DNA repair to facilitate the faithful disjunction of sister chromatids in anaphase. These include the Bloom's syndrome gene product, BLM and its partners, as well as a number of proteins that are ..
  12. ncbi How proteolysis drives the cell cycle
    R W King
    Department of Cell Biology, Harvard Medical School, 240 Longwood Ave, Boston, MA 02115, USA
    Science 274:1652-9. 1996
    ..CDK activators or inhibitors-and also how proteolysis may directly trigger the transition from metaphase to anaphase. Proteolysis during the cell cycle is mediated by two distinct ubiquitin-conjugation pathways...
  13. ncbi MASTL is the human orthologue of Greatwall kinase that facilitates mitotic entry, anaphase and cytokinesis
    Erik Voets
    Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Cell Cycle 9:3591-601. 2010
    ..RNAi cells that enter and progress through mitosis often fail to completely separate their sister chromatids in anaphase. This causes chromatin to be trapped in the cleavage furrow, which may lead to the formation of 4N G(1) cells by ..
  14. pmc Budding yeast chromosome structure and dynamics during mitosis
    C G Pearson
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Cell Biol 152:1255-66. 2001
    ..images, sister centromeres in budding yeast are found to be separated and oscillate between spindle poles before anaphase B spindle elongation...
  15. pmc Functional analysis of human microtubule-based motor proteins, the kinesins and dyneins, in mitosis/cytokinesis using RNA interference
    Changjun Zhu
    The Burnham Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 16:3187-99. 2005
    ..for chromosome congression and alignment; Kif4A and Kif4B (members of the kinesin-4 family) have roles in anaphase spindle dynamics; and Kif4A, Kif4B, MKLP1, and MKLP2 (members of the kinesin-6 family) are essential for ..
  16. pmc Aurora B couples chromosome alignment with anaphase by targeting BubR1, Mad2, and Cenp-E to kinetochores
    Claire Ditchfield
    School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Rd, Manchester M13 9PT, UK
    J Cell Biol 161:267-80. 2003
    ..Together, these results suggest that by targeting checkpoint proteins to kinetochores, Aurora B couples chromosome alignment with anaphase onset.
  17. pmc Ordered proteolysis in anaphase inactivates Plk1 to contribute to proper mitotic exit in human cells
    Catherine Lindon
    Wellcome Trust Cancer Research UK Gurdon Institute, Tennis Court Rd, Cambridge CB2 1QR, England, UK
    J Cell Biol 164:233-41. 2004
    ..that two of these regulators, polo-like kinase 1 (Plk1) and Aurora A, are degraded at different times after the anaphase-promoting complex/cyclosome (APC/C) switches from binding Cdc20 to Cdh1...
  18. pmc Cdk1-dependent destruction of Eco1 prevents cohesion establishment after S phase
    Nicholas A Lyons
    Department of Physiology, University of California, San Francisco, San Francisco, CA 94158, USA
    Mol Cell 42:378-89. 2011
    ..We also show that deregulation of Eco1 results in chromosome separation defects in anaphase. We conclude that this regulatory mechanism helps optimize the level of sister chromatid cohesion, ensuring a ..
  19. pmc A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans
    Anjon Audhya
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 171:267-79. 2005
    ..This cytokinesis failure likely results from an anaphase spindle defect in which interzonal microtubule bundles that recruit Aurora B kinase and the kinesin, ZEN-4, fail ..
  20. pmc Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2
    Silke Hauf
    Research Institute of Molecular Pathology, Vienna, Austria
    PLoS Biol 3:e69. 2005
    ..separase releases the complex from chromosomes and thereby enables the separation of sister chromatids in anaphase. In vertebrate cells, the bulk of cohesin dissociates from chromosome arms already during prophase and ..
  21. ncbi Hos1 deacetylates Smc3 to close the cohesin acetylation cycle
    Vanessa Borges
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, London WC2A 3PX, UK
    Mol Cell 39:677-88. 2010
    ..At the time of anaphase, Smc3 loses its acetylation again, but the Smc3 deacetylase and the possible importance of Smc3 deacetylation are ..
  22. pmc Sli15(INCENP) dephosphorylation prevents mitotic checkpoint reengagement due to loss of tension at anaphase onset
    Lesia Mirchenko
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London, UK
    Curr Biol 20:1396-401. 2010
    The mitotic checkpoint, also known as the spindle assembly checkpoint, delays anaphase onset until all chromosomes have reached bipolar tension on the mitotic spindle [1-3]...
  23. ncbi The centromeric protein Sgo1 is required to sense lack of tension on mitotic chromosomes
    Vahan B Indjeian
    Department of Molecular and Cellular Biology, Biological Laboratories, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Science 307:130-3. 2005
    ..thus required for sensing tension between sister chromatids during mitosis, and its degradation when they separate may prevent cell cycle arrest and chromosome loss in anaphase, a time when sister chromatids are no longer under tension.
  24. pmc Sds22 and Repo-Man stabilize chromosome segregation by counteracting Aurora B on anaphase kinetochores
    Claudia Wurzenberger
    Institute of Biochemistry, Swiss Federal Institute of Technology Zurich, CH 8093 Zurich, Switzerland
    J Cell Biol 198:173-83. 2012
    ..During anaphase, however, kinetochore-microtubule attachments must be maintained despite a drop of tension after removal of ..
  25. ncbi Dual inhibition of sister chromatid separation at metaphase
    O Stemmann
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 107:715-26. 2001
    Separation of sister chromatids in anaphase is mediated by separase, an endopeptidase that cleaves the chromosomal cohesin SCC1. Separase is inhibited by securin, which is degraded at the metaphase-anaphase transition...
  26. ncbi Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor
    Aaron F Straight
    Department of Cell Biology and Institute of Chemistry and Cell Biology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA
    Science 299:1743-7. 2003
    ..Continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components...
  27. pmc Mps1 directs the assembly of Cdc20 inhibitory complexes during interphase and mitosis to control M phase timing and spindle checkpoint signaling
    John Maciejowski
    Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    J Cell Biol 190:89-100. 2010
    ..The spindle assembly checkpoint (SAC) in mammals uses cytosolic and kinetochore-based signaling pathways to inhibit anaphase. In this study, we use chemical genetics to show that the protein kinase Mps1 regulates both aspects of the SAC...
  28. ncbi Securin is required for chromosomal stability in human cells
    P V Jallepalli
    The Johns Hopkins Oncology Center, 1650 Orleans Street, Baltimore, MD 21231, USA
    Cell 105:445-57. 2001
    ..These results illuminate the function of mammalian securin and show that it is essential for the maintenance of euploidy...
  29. ncbi A minimal midzone protein module controls formation and length of antiparallel microtubule overlaps
    Peter Bieling
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Cell 142:420-32. 2010
    ..In anaphase, this antiparallel microtubule array is tightly bundled forming the midzone, which serves as a hub for the ..
  30. pmc The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore-microtubule attachment and in maintaining the spindle assembly checkpoint
    Silke Hauf
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    J Cell Biol 161:281-94. 2003
    ..Mammalian cells treated with Hesperadin enter anaphase in the presence of numerous monooriented chromosomes, many of which may have both sister kinetochores attached to ..
  31. pmc Error-prone mammalian female meiosis from silencing the spindle assembly checkpoint without normal interkinetochore tension
    Agnieszka Kolano
    Centre Interdisciplinaire de Recherche en Biologie, Unité Mixte de Recherche Centre National de la Recherche Scientifique 7241 Institut National de la Santé et de la Recherche Médicale U1050, College de France, 75005 Paris, France
    Proc Natl Acad Sci U S A 109:E1858-67. 2012
    ..Despite this, the spindle assembly checkpoint is normally silenced and the advance to anaphase I and first polar body extrusion takes place without delay...
  32. pmc Cortical dynein and asymmetric membrane elongation coordinately position the spindle in anaphase
    Tomomi Kiyomitsu
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
    Cell 154:391-402. 2013
    ..Here, we demonstrate that two mechanisms act coordinately to center the spindle during anaphase in symmetrically dividing human cells...
  33. ncbi Deletion of Mia1/Alp7 activates Mad2-dependent spindle assembly checkpoint in fission yeast
    Masamitsu Sato
    Nat Cell Biol 5:764-6; author reply 766. 2003
  34. ncbi Non-mitotic functions of the Anaphase-Promoting Complex
    Manuel Eguren
    Cell Division and Cancer Group, Spanish National Cancer Research Center CNIO, Madrid, Spain
    Semin Cell Dev Biol 22:572-8. 2011
    The Anaphase-Promoting Complex or Cyclosome (APC/C) is an E3 ubiquitin ligase whose activation requires the binding of a cofactor, either Cdc20 or Cdh1...
  35. pmc Functional central spindle assembly requires de novo microtubule generation in the interchromosomal region during anaphase
    Ryota Uehara
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
    J Cell Biol 191:259-67. 2010
    The central spindle forms between segregating chromosomes during anaphase and is required for cytokinesis...
  36. ncbi A midzone-based ruler adjusts chromosome compaction to anaphase spindle length
    Gabriel Neurohr
    Centre for Genomic Regulation CRG, Barcelona, Spain
    Science 332:465-8. 2011
    ..The spindle midzone, aurora/Ipl1 activity, and Ser10 of histone H3 mediated this response. Thus, the anaphase spindle may function as a ruler to adapt the condensation of chromatids, promoting their segregation regardless ..
  37. pmc The Lrs4-Csm1 monopolin complex associates with kinetochores during anaphase and is required for accurate chromosome segregation
    Ilana L Brito
    David H Koch Institute for Integrative Cancer Research and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA, USA
    Cell Cycle 9:3611-8. 2010
    ..This kinetochore localization is observed during anaphase and depends on the on the Mitotic Exit Network, a signaling cascade essential for the completion of mitosis...
  38. pmc A mechanism linking extra centrosomes to chromosomal instability
    Neil J Ganem
    Howard Hughes Medical Institute, Department of Pediatric Oncology, Dana Farber Cancer Institute, Children s Hospital, Boston, Massachusetts 02115, USA
    Nature 460:278-82. 2009
    ..It has been proposed that extra centrosomes generate CIN by promoting multipolar anaphase, a highly abnormal division that produces three or more aneuploid daughter cells...
  39. pmc Welcome to a new kind of tension: translating kinetochore mechanics into a wait-anaphase signal
    Thomas J Maresca
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Sci 123:825-35. 2010
    ..the interaction between chromosomes and the plus-ends of dynamic spindle microtubules and control the timing of anaphase onset by regulating the spindle assembly checkpoint (SAC)...
  40. pmc KNL1/Spc105 recruits PP1 to silence the spindle assembly checkpoint
    Jessica S Rosenberg
    Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065, USA
    Curr Biol 21:942-7. 2011
    The spindle assembly checkpoint (SAC) delays anaphase onset until kinetochores accomplish bioriented microtubule attachments [1]...
  41. pmc Sequential assembly of myosin II, an IQGAP-like protein, and filamentous actin to a ring structure involved in budding yeast cytokinesis
    J Lippincott
    Department of Cell Biology, Harvard Medical School, Boston, Massachussetts 02115, USA
    J Cell Biol 140:355-66. 1998
    ..most of the cell cycle but localizes to a ring structure at the mother-bud junction after the initiation of anaphase. This ring contains filamentous actin and Myo1p, a myosin II homologue...
  42. ncbi Relocation of the chromosomal passenger complex prevents mitotic checkpoint engagement at anaphase
    María Dolores Vázquez-Novelle
    Cell Division and Aneuploidy Laboratory, Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire, UK
    Curr Biol 20:1402-7. 2010
    The mitotic checkpoint monitors the attachment of kinetochores to microtubules and delays anaphase onset until all sister kinetochores have become attached to opposite poles [1, 2]...
  43. pmc Condensin II resolves chromosomal associations to enable anaphase I segregation in Drosophila male meiosis
    Tom A Hartl
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona, United States of America
    PLoS Genet 4:e1000228. 2008
    ..In mutants of either subunit, territory formation fails and chromatin is dispersed throughout the nucleus. Anaphase I is also abnormal in Cap-H2 mutants as chromatin bridges are found between segregating heterologous and ..
  44. pmc The budding yeast Ipl1/Aurora protein kinase regulates mitotic spindle disassembly
    Stéphanie Buvelot
    Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Cell Biol 160:329-39. 2003
    ..from G1 to metaphase, transfers to the spindle after metaphase, and accumulates at the spindle midzone late in anaphase. Ipl1p kinase activity increases at anaphase, and ipl1 mutants can stabilize fragile spindles...
  45. pmc Mitotic CDKs control the metaphase-anaphase transition and trigger spindle elongation
    Rami Rahal
    Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 USA
    Genes Dev 22:1534-48. 2008
    ..We show that Clb-CDKs are important for the activation of the ubiquitin ligase Anaphase-Promoting Complex/Cyclosome (APC/C)-Cdc20 that triggers the metaphase-anaphase transition...
  46. pmc Dynein at the kinetochore: Timing, Interactions and Functions
    Jason R Bader
    Department of Biological Sciences, University of Notre Dame, Galvin Life Sciences Center, Notre Dame, IN 46556, United States
    Semin Cell Dev Biol 21:269-75. 2010
    ..chromosome alignment, including initial microtubule (MT) capture, monitoring MT attachments, prometaphase and anaphase chromosome movement and tension generation at metaphase...
  47. ncbi Cohesin cleavage by separase required for anaphase and cytokinesis in human cells
    S Hauf
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Science 293:1320-3. 2001
    Cell division depends on the separation of sister chromatids in anaphase. In yeast, sister separation is initiated by cleavage of cohesin by the protease separase...
  48. pmc Mad2 prevents aneuploidy and premature proteolysis of cyclin B and securin during meiosis I in mouse oocytes
    Hayden A Homer
    Newcastle Fertility Centre at Life, Centre for Life, Newcastle upon Tyne NE1 4EP, United Kingdom
    Genes Dev 19:202-7. 2005
    In mitosis, the spindle checkpoint protein Mad2 averts aneuploidy by delaying anaphase onset until chromosomes align. Here we show that depletion of Mad2 in meiosis I mouse oocytes induced an increased incidence of aneuploidy...
  49. ncbi Cdc14 inhibits transcription by RNA polymerase I during anaphase
    Andres Clemente-Blanco
    Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College, Du Cane Road, London W12 0NN, UK
    Nature 458:219-22. 2009
    ..for nucleolar segregation and mitotic exit, inhibits transcription of yeast ribosomal genes (rDNA) during anaphase. The phosphatase activity of Cdc14 is required for RNA polymerase I (Pol I) inhibition in vitro and in vivo...
  50. ncbi Cleavage of cohesin by the CD clan protease separin triggers anaphase in yeast
    F Uhlmann
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 103:375-86. 2000
    ..replicated DNA strands remain physically connected until their segregation to opposite poles of the cell during anaphase. This "sister chromatid cohesion" is essential for the alignment of chromosomes on the mitotic spindle during ..
  51. pmc The degradation of two mitotic cyclins contributes to the timing of cytokinesis
    Arnaud Echard
    Department of Biochemistry and Biophysics, University of California, San Francisco, Genentech Hall, 600 16th Street, San Francisco, CA 94143, USA
    Curr Biol 13:373-83. 2003
    ..We examined the interface between mitotic cyclin destruction and the timing of cytokinesis...
  52. ncbi Resolution of anaphase bridges in cancer cells
    Diane R Hoffelder
    Department of Biological Sciences, University of Pittsburgh, 258 Crawford Hall, 15260, Pittsburgh, PA 15260, USA
    Chromosoma 112:389-97. 2004
    ..An indicator of unstable chromosomes is the presence of chromatin bridges during anaphase. We examined in detail the fate of anaphase bridges in cultured oral squamous cell carcinoma cells in real-time...
  53. pmc DNA damage checkpoints inhibit mitotic exit by two different mechanisms
    Fengshan Liang
    Department of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Call Street, Tallahassee, FL 32306, USA
    Mol Cell Biol 27:5067-78. 2007
    ..In the budding yeast Saccharomyces cerevisiae, mitotic exit is controlled by the FEAR (for "Cdc-fourteen early anaphase release") and mitotic exit network (MEN) pathways...
  54. ncbi Polo kinase regulates mitotic chromosome condensation by hyperactivation of condensin DNA supercoiling activity
    Julie St-Pierre
    Institute for Research in Immunology and Cancer, Universite de Montreal, Station Centre Ville, Quebec, Canada
    Mol Cell 34:416-26. 2009
    ..Here we show, using budding yeast, that condensin is regulated by phosphorylation specifically in anaphase. This phosphorylation depends on several mitotic regulators, and the ultimate effector is the Polo kinase Cdc5...
  55. pmc Kinesin-5 acts as a brake in anaphase spindle elongation
    Adam M Saunders
    Curr Biol 17:R453-4. 2007
  56. pmc Ultrafine anaphase bridges, broken DNA and illegitimate recombination induced by a replication fork barrier
    Sevil Sofueva
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK
    Nucleic Acids Res 39:6568-84. 2011
    ..persistence of replication intermediates (most likely regions of unreplicated DNA) into mitosis, which results in anaphase bridge formation and ultimately to DNA breakage...
  57. pmc Molecular networks linked by Moesin drive remodeling of the cell cortex during mitosis
    Chantal Roubinet
    Cell Biology of Mitosis laboratory, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    J Cell Biol 195:99-112. 2011
    ..phosphatase restricts high Moesin activity to early mitosis and down-regulates Moesin at the polar cortex, after anaphase onset...
  58. pmc The fission yeast septation initiation network (SIN) kinase, Sid2, is required for SIN asymmetry and regulates the SIN scaffold, Cdc11
    Anna Feoktistova
    Howard Hughes Medical Institute and Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37212, USA
    Mol Biol Cell 23:1636-45. 2012
    ..SIN activity requires the asymmetric distribution of its signaling components to one of the two SPBs during anaphase, and Cdc11 hyperphosphorylation correlates with proficient SIN activity...
  59. pmc The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis
    Sarah Maerki
    Institute of Biochemistry, Swiss Federal Institute of Technology Zurich, CH 8093 Zurich, Switzerland
    J Cell Biol 187:791-800. 2009
    ..and regulates translocation of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase, similar to the previously described BTB-Kelch proteins KLHL9 and KLHL13...
  60. pmc Rising cyclin-CDK levels order cell cycle events
    Catherine Oikonomou
    Laboratory of Cell Cycle Genetics, The Rockefeller University, New York, New York, United States of America
    PLoS ONE 6:e20788. 2011
    ....
  61. ncbi Breakage of the nuclear envelope by an extending mitotic nucleus occurs during anaphase in Schizosaccharomyces japonicus
    Keita Aoki
    Microbial Genetics Laboratory, Genetic Strains Research Center, National Institute of Genetics, Mishima, Shizuoka, Japan
    Genes Cells 16:911-26. 2011
    ..The mitotic nucleus of Sz. japonicus adopted a fusiform shape in anaphase, and its following extension caused separation. Finally, a tear in the nuclear envelope occurred in late anaphase...
  62. pmc Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore
    Lucy J Bock
    European Institute of Oncology, Department of Experimental Oncology, Via Adamello 16, 20139 Milan, Italy
    Nat Cell Biol 14:614-24. 2012
    ..Cnn1 regulates KMN activity in a spatiotemporal manner by inhibiting the interaction between its complexes. Cnn1 activity peaks in anaphase and is driven by the Cdc28, Mps1 and Ipl1 kinases.
  63. ncbi Cell-size-dependent spindle elongation in the Caenorhabditis elegans early embryo
    Yuki Hara
    Cell Architecture Laboratory, Center for Frontier Research, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411 8540, Japan
    Curr Biol 19:1549-54. 2009
    ..During anaphase, the mitotic spindle elongates and delivers the centrosomes and sister chromatids near the centers of the nascent ..
  64. pmc Topoisomerase II- and condensin-dependent breakage of MEC1ATR-sensitive fragile sites occurs independently of spindle tension, anaphase, or cytokinesis
    Nadia Hashash
    Division of Stem Cell Biology and Developmental Genetics, National Institute for Medical Research, Medical Research Council, London, United Kingdom
    PLoS Genet 8:e1002978. 2012
    ..including errors in replication fork restart, premature mitotic chromosome condensation, spindle tension, anaphase, and cytokinesis...
  65. ncbi The dynamics of homologous chromosome pairing during male Drosophila meiosis
    Julio Vazquez
    Department of Biochemistry and Biophysics, University of California, San Francisco, 94143, USA
    Curr Biol 12:1473-83. 2002
    ..for the proper orientation of chromosomes at the metaphase plate and their subsequent disjunction during anaphase I...
  66. pmc The chromokinesin, KLP3A, dives mitotic spindle pole separation during prometaphase and anaphase and facilitates chromatid motility
    Mijung Kwon
    University of California Davis, Davis, California 95616, USA
    Mol Biol Cell 15:219-33. 2004
    ..to spindle pole separation during the prometaphase-to-metaphase transition (when it antagonizes Ncd) and anaphase B, to normal rates of chromatid motility during anaphase A, and to the proper spacing of daughter nuclei during ..
  67. pmc PICH and BLM limit histone association with anaphase centromeric DNA threads and promote their resolution
    Yuwen Ke
    Deparment of Pharmacology, Howard Hughes Medical Institute, Dallas, TX, USA
    EMBO J 30:3309-21. 2011
    ..helicase decorate ultrafine histone-negative DNA threads that link the segregating sister centromeres during anaphase. The functions of PICH and BLM at these threads are not understood, however...
  68. pmc System-level feedbacks make the anaphase switch irreversible
    Enuo He
    Oxford Centre for Integrative Systems Biology, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
    Proc Natl Acad Sci U S A 108:10016-21. 2011
    ..checkpoint prevents a eukaryotic cell from commencing to separate its replicated genome into two daughter cells (anaphase) until all of its chromosomes are properly aligned on the metaphase plate, with the two copies of each chromosome ..
  69. pmc Control of dTTP pool size by anaphase promoting complex/cyclosome is essential for the maintenance of genetic stability
    Po Yuan Ke
    Graduate Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei
    Genes Dev 19:1920-33. 2005
    b>Anaphase promoting complex/cyclosome (APC/C)-mediated proteolysis is essential for chromosome segregation, mitotic exit, and G1 entry. Here, we show the importance of APC/C in the control of dTTP pool size in mammalian cells...
  70. pmc Analysis of the mitotic exit control system using locked levels of stable mitotic cyclin
    Benjamin J Drapkin
    Laboratory of Yeast Molecular Genetics, The Rockefeller University, New York, NY 10021, USA
    Mol Syst Biol 5:328. 2009
    Cyclin-dependent kinase (Cdk) both promotes mitotic entry (spindle assembly and anaphase) and inhibits mitotic exit (spindle disassembly and cytokinesis), leading to an elegant quantitative hypothesis that a single cyclin oscillation can ..
  71. ncbi Cell cycle mechanisms of sister chromatid separation; roles of Cut1/separin and Cut2/securin
    M Yanagida
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwakecho, Sakyo ku, Kyoto 606 8502, Japan
    Genes Cells 5:1-8. 2000
    ..Separation and segregation of sister chromatids in growing cells occurs in the cell cycle stage called 'anaphase'...
  72. pmc Condensins promote chromosome recoiling during early anaphase to complete sister chromatid separation
    Matthew J Renshaw
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, UK
    Dev Cell 19:232-44. 2010
    Sister chromatid separation is initiated at anaphase onset by the activation of separase, which removes cohesins from chromosomes. However, it remains elusive how sister chromatid separation is completed along the entire chromosome length...
  73. ncbi Kinesin-related cut7 protein associates with mitotic and meiotic spindles in fission yeast
    I Hagan
    Department of Biophysics, Faculty of Science, Kyoto University, Japan
    Nature 356:74-6. 1992
    ..mitotic and meiotic nuclear division and associates with mitotic spindle microtubules in a stage-specific manner, associating with the mid-anaphase B midzone. In cut7ts mutants, spindle pole bodies stain but mitotic microtubules do not.
  74. ncbi APC/C Cdh1 targets aurora kinase to control reorganization of the mitotic spindle at anaphase
    Suzanne Floyd
    Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Curr Biol 18:1649-58. 2008
    Control of mitotic cell cycles by the anaphase-promoting complex or cyclosome (APC/C) ubiquitin ligase depends on its coactivators Cdc20 and Cdh1...
  75. pmc The polarity and dynamics of microtubule assembly in the budding yeast Saccharomyces cerevisiae
    P S Maddox
    Department of Biology, CB3280, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Nat Cell Biol 2:36-41. 2000
    ..After anaphase onset, photobleached marks in the interpolar spindle are persistent and do not move relative to the SPBs...
  76. ncbi Polo-like kinases: a team that plays throughout mitosis
    D M Glover
    Department of Anatomy and Physiology, Medical Sciences Institute, University of Dundee, UK
    Genes Dev 12:3777-87. 1998
  77. ncbi Maximal chromosome compaction occurs by axial shortening in anaphase and depends on Aurora kinase
    Felipe Mora-Bermúdez
    Gene Expression and Cell Biology Unit, European Molecular Biology Laboratory, Meyerhofstr 1, 69117 Heidelberg, Germany
    Nat Cell Biol 9:822-31. 2007
    ..Surprisingly, maximal compaction was not reached in metaphase, but in late anaphase, after sister chromatid segregation...
  78. ncbi A conditional lethal mutant in the fission yeast 26 S protease subunit mts3+ is defective in metaphase to anaphase transition
    C Gordon
    Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh, Scotland, United Kingdom
    J Biol Chem 271:5704-11. 1996
    ..is resistant to the mitotic poison MBC and at the restrictive temperature is defective in metaphase to anaphase transition. The predicted amino acid sequence of mts3+ is 36% identical with the budding yeast gene NIN1...
  79. ncbi Abnormal cytokinesis in cells deficient in the breast cancer susceptibility protein BRCA2
    Matthew J Daniels
    University of Cambridge, Cancer Research UK, Department of Oncology and the Medical Research Council MRC Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 2XZ, UK
    Science 306:876-9. 2004
    ..Our findings thus link cytokinetic abnormalities to a hereditary cancer syndrome characterized by chromosomal instability and may help to explain why BRCA2-deficient tumors are frequently aneuploid...
  80. pmc Essential global role of CDC14 in DNA synthesis revealed by chromosome underreplication unrecognized by checkpoints in cdc14 mutants
    Stanimir Dulev
    National Institutes of Health, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Proc Natl Acad Sci U S A 106:14466-71. 2009
    ..crucial for accurate chromosome segregation, which is exemplified by its absolute requirement in yeast for the anaphase segregation of nucleolar organizers; however the nature of this essential pathway is not understood...
  81. ncbi Depletion of topoisomerase IIalpha leads to shortening of the metaphase interkinetochore distance and abnormal persistence of PICH-coated anaphase threads
    Jennifer M Spence
    Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
    J Cell Sci 120:3952-64. 2007
    ....
  82. pmc DNA damage activates the SAC in an ATM/ATR-dependent manner, independently of the kinetochore
    Eun Mi Kim
    Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, Charlottesville, Virginia, United States of America
    PLoS Genet 4:e1000015. 2008
    ..Here we show that yeast cells lacking the DNA damage checkpoint arrest prior to anaphase in response to low doses of the DNA damaging agent methyl methane sulfonate (MMS)...
  83. pmc APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex
    Z Tang
    Departments of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9041, USA
    Mol Biol Cell 12:3839-51. 2001
    In mitosis, the anaphase-promoting complex (APC) regulates the onset of sister-chromatid separation and exit from mitosis by mediating the ubiquitination and degradation of the securin protein and mitotic cyclins...
  84. pmc The Drosophila Geminin homolog: roles for Geminin in limiting DNA replication, in anaphase and in neurogenesis
    L M Quinn
    Peter MacCallum Cancer Institute, Trescowthick Research Laboratories, Locked Bag 1, Melbourne, Victoria 8006, Australia
    Genes Dev 15:2741-54. 2001
    ..Dm Geminin is present in cycling cells; protein accumulates during S phase and is degraded at the metaphase to anaphase transition...
  85. pmc Use of the novel Plk1 inhibitor ZK-thiazolidinone to elucidate functions of Plk1 in early and late stages of mitosis
    Anna Santamaria
    Department of Cell Biology and Intracellular Protein Transport, Independent Junior Research Group, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
    Mol Biol Cell 18:4024-36. 2007
    ..Finally, we show that Plk1 activity is essential for cleavage furrow formation and ingression, leading to successful cytokinesis...
  86. ncbi KEN-box-dependent degradation of the Bub1 spindle checkpoint kinase by the anaphase-promoting complex/cyclosome
    Wei Qi
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 282:3672-9. 2007
    ..In response to misaligned chromosomes, Bub1 directly inhibits the ubiquitin ligase activity of the anaphase-promoting complex or cyclosome (APC/C) by phosphorylating its activator Cdc20...
  87. pmc Disappearance of the budding yeast Bub2-Bfa1 complex from the mother-bound spindle pole contributes to mitotic exit
    Roberta Fraschini
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, 20126 Milano, Italy
    J Cell Biol 172:335-46. 2006
    ..Bub2 and Bfa1 are found on both duplicated spindle pole bodies until anaphase onset, when they disappear from the mother-bound spindle pole under unperturbed conditions...
  88. pmc Time-lapse microscopy reveals unique roles for kinesins during anaphase in budding yeast
    A F Straight
    Department of Physiology, School of Medicine, University of California San Francisco, San Francisco, California 94143, USA
    J Cell Biol 143:687-94. 1998
    ..functional overlap, each motor mutant has a specific defect in mitosis: cin8Delta mutants lack the rapid phase of anaphase B, kip1Delta mutants show defects in the slow phase of anaphase B, and kip3Delta mutants prolong the duration of ..
  89. pmc Kinase activity of fission yeast Mph1 is required for Mad2 and Mad3 to stably bind the anaphase promoting complex
    Judith Zich
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, EH9 3JR, UK
    Curr Biol 22:296-301. 2012
    ..The spindle checkpoint delays anaphase onset until all chromosomes are attached to spindle microtubules in a bipolar fashion [3, 4]...
  90. ncbi Kid-mediated chromosome compaction ensures proper nuclear envelope formation
    Miho Ohsugi
    Division of Oncology, Department of Cancer Biology, The University of Tokyo, Minato ku, Tokyo, 108 8639, Japan
    Cell 132:771-82. 2008
    ..cells lacking the expression of chromokinesin Kid/kinesin-10, we show that Kid localizes to the boundaries of anaphase and telophase chromosomes and contributes to the shortening of the anaphase chromosome mass along the spindle ..
  91. pmc Nsk1 ensures accurate chromosome segregation by promoting association of kinetochores to spindle poles during anaphase B
    Graham J Buttrick
    Division of Biomedical Cell Biology, Warwick Medical School, University of Warwick, Coventry CV4 7AL, United Kingdom
    Mol Biol Cell 22:4486-502. 2011
    ..Nsk1 localizes to the interface between kinetochores and the inner face of the spindle pole body during anaphase. In the absence of Nsk1, some kinetochores become detached from spindle poles during anaphase B...
  92. pmc Probing the in vivo function of Mad1:C-Mad2 in the spindle assembly checkpoint
    Luca L Fava
    Growth and Development, Biozentrum, University of Basel, Basel, Switzerland
    EMBO J 30:3322-36. 2011
    The spindle assembly checkpoint (SAC) restrains anaphase until all chromosomes become bi-oriented on the mitotic spindle. The SAC protein Mad2 can fold into two distinct conformers, open (O) and closed (C), and can asymmetrically dimerize...
  93. ncbi Getting down to the phosphorylated 'nuts and bolts' of spindle checkpoint signalling
    Judith Zich
    Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh, UK
    Trends Biochem Sci 35:18-27. 2010
    ..Specifically, it delays anaphase onset until all chromosomes are attached in a bi-oriented fashion to spindle microtubules...
  94. ncbi Centromere DNA decatenation depends on cohesin removal and is required for mammalian cell division
    Lily Hui Ching Wang
    Department of Cell Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Cell Sci 123:806-13. 2010
    ..The recent visualization of PICH (Plk1-interacting checkpoint helicase)-coated DNA threads in anaphase cells raises new questions as to the role of DNA catenation and its regulation in time and space...
  95. ncbi Anaphase DNA bridges induced by lack of RecQ5 in Drosophila syncytial embryos
    Haruna Sakurai
    Division of Biochemistry, Faculty of Pharmaceutical Science, Setsunan University, Hirakata, Osaka, Japan
    FEBS Lett 585:1923-8. 2011
    ..We demonstrate that the mitotic defects are derived from anaphase DNA bridges...
  96. pmc Asymmetric division of cyst stem cells in Drosophila testis is ensured by anaphase spindle repositioning
    Jun Cheng
    Department of Biomedical Engineering, Center for Ultrafast Optical Science, 1101 Beal Ave, Ann Arbor, MI 48109, USA
    Development 138:831-7. 2011
    ..Here, we report that CySCs divide asymmetrically through repositioning the mitotic spindle around anaphase. CySC spindle repositioning requires functional centrosomes, Dynein and the actin-membrane linker Moesin...
  97. pmc Anaphase catastrophe is a target for cancer therapy
    Fabrizio Galimberti
    Department of Pharmacology and Toxicology, Norris Cotton Cancer Center, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Clin Cancer Res 17:1218-22. 2011
    ..and forces chromosomes to segregate to multiple daughter cells, an event triggering apoptosis that we refer to as anaphase catastrophe. Anaphase catastrophe specifically kills tumor cells with more than 2 centrosomes...
  98. pmc A conserved cyclin-binding domain determines functional interplay between anaphase-promoting complex-Cdh1 and cyclin A-Cdk2 during cell cycle progression
    C S Sørensen
    Institute of Cancer Biology, Danish Cancer Society, DK 2100 Copenhagen Ø, Denmark
    Mol Cell Biol 21:3692-703. 2001
    Periodic activity of the anaphase-promoting complex (APC) ubiquitin ligase determines progression through multiple cell cycle transitions by targeting cell cycle regulators for destruction...
  99. pmc Mechanisms for concentrating Rho1 during cytokinesis
    Satoshi Yoshida
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Division of Hematology Oncology, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    Genes Dev 23:810-23. 2009
    ..We find that budding yeast utilizes two mechanisms to concentrate Rho1 at the division site. During anaphase, the primary mechanism for recruiting Rho1 is binding to its guanine nucleotide exchange factors (GEFs)...
  100. ncbi SKAP associates with kinetochores and promotes the metaphase-to-anaphase transition
    Lin Fang
    Department of Biological Sciences, Stanford University, Stanford, CA, USA
    Cell Cycle 8:2819-27. 2009
    The spindle assembly checkpoint (SAC) controls the anaphase onset by preventing premature chromosome segregation until bipolar microtubule (MT) attachment and inter-kinetochore tension are fully established for every kinetochore pair...
  101. ncbi The anaphase promoting complex induces substrate degradation during neuronal differentiation
    Dympna Harmey
    Department of Cancer Biology, Scripps Florida, The Scripps Research Institute, Jupiter, Florida 33458
    J Biol Chem 284:4317-23. 2009
    The anaphase promoting complex (APC) is an E3 ubiquitin ligase required for the metaphase-to-anaphase transition and mitotic exit...

Research Grants67

  1. The Role of Cks Proteins in Mammalian Meiosis
    Charles H Spruck; Fiscal Year: 2010
    ..processes including: 1) activation of MPF kinase activity;2) activation of the multi-subunit ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C);3) proteolysis of cyclin B1;and 4) transcriptional activation of APC/C ..
  2. Cell Cycle Regulation and Adult T Cell Leukemia
    Chou Zen Giam; Fiscal Year: 2013
    ..We have shown that Tax can activate the anaphase promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase that controls metaphase to anaphase transition and ..
  3. MECHANISMS OF DENDRITE MORPHOGENESIS BY THE ANAPHASE-PROMOTING COMPLEX
    ALBERT HONG JAE KIM; Fiscal Year: 2013
    ..The candidate previously discovered that strikingly, major mitotic ubiquitin ligase Cdc20-Anaphase- Promoting Complex (Cdc20-APC) is required for dendrite morphogenesis in post-mitotic neurons of the brain...
  4. ATM Controls Genomic Stability in Pluripotent Stem Cells
    JASON MARK BECKTA; Fiscal Year: 2013
    ..studies (accomplished preliminarily in non-transformed normal fibroblasts) revealed a prolonged metaphase-to-anaphase transition time after ATM inhibition...
  5. Mechanisms of arsenic-induced chromosomal instability and carcinogenesis
    Wei Dai; Fiscal Year: 2013
    ..by suppressing the activation of BubR1, a cell cycle checkpoint protein, leading to unscheduled activation of anaphase promoting complex/cyclosome (APC/C) in melanoma and HeLa cells...
  6. HTLV-I Tax activates the anaphase promoting complex
    Chou Zen Giam; Fiscal Year: 2013
    ..We have shown that the Tax can activate the anaphase promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase that controls metaphase to anaphase transition and ..
  7. CELL CYCLE CONTROL IN EARLY DROSOPHILA DEVELOPMENT
    Terry L Orr-Weaver; Fiscal Year: 2013
    ..A meiotic form of the Anaphase Promoting Complex/Cyclosome, APC/CCort, controls proteolysis essential for the completion of meiosis in the egg...
  8. Regulation of chromosome segregation by SMC complexes and Top2 in S. pombe
    MATTHEW J O'CONNELL; Fiscal Year: 2013
    ..body of preliminary data that links the mitotic defects of smc6 mutants following DNA damage to a defect in pre-anaphase cohesin removal. Here, we address cause versus consequence and the spatiotemporal regulation of cohesin dynamics...
  9. Investigation of Anaphase Promoting Complex function in synaptic transmission
    Jennifer Kowalski; Fiscal Year: 2012
    ..Recent work established that the Anaphase Promoting Complex (APC), an ubiquitin ligase that controls protein abundance in the cell cycle, also regulates ..
  10. Function of the chromosomal kinase haspin in mitosis
    JONATHAN M HIGGINS; Fiscal Year: 2010
    ..on chromosome arms in late G2/early prophase, becomes focused at centromeres by prometaphase, and declines during anaphase. In vitro, haspin specifically phosphorylates histone H3 at Thr-3, and depletion of haspin by RNA interference (..
  11. Regulation of SMN and Identification of its Downstream Target
    Judith Jebanathirajah Steen; Fiscal Year: 2013
    ..Our preliminary results show that SMN is found in a complex containing an E3 ubiquitin ligase the Anaphase- Promoting Complex (APC) and HuD, a RNA binding protein...
  12. Kinetochore Function and Cell Cycle Progression Revision
    Katsumi Kitagawa; Fiscal Year: 2012
    ..The spindle checkpoint is deactivated and should not be activated during anaphase. Mutual inhibition between the anaphase-promoting complex (APC) and Mps1, an essential component of the spindle ..
  13. CARP-1: A Potential Therapeutic Agent for Breast Cancer
    ARUN KUMAR RISHI; Fiscal Year: 2013
    ..A yeast-two-hybrid (Y2H) screen revealed that CARP- 1 binds with the E3 ubiquitin ligase subunit anaphase promoting complex (APC)-2 protein...
  14. Arsenic Induced Miotic Arrest Associated Apoptosis
    J Christopher States; Fiscal Year: 2009
    ..Arsenite, the carcinogenic form of arsenic found in drinking water, disrupts mitosis causing an anaphase delay and induces aneuploidy in normal diploid human fibroblasts and peripheral blood lymphocytes, and mitotic ..
  15. Characterization of JNK in Cell Cycle Control
    ZE apos EV A RONAI; Fiscal Year: 2012
    ..Such targeting is mediated by Cdh1, which recruits the Anaphase Promoting Complex or Cyclosome (APC/C) ubiquitin ligase to ubiquitinate JNK, resulting in its proteasome-..
  16. Study of Anti-Survival signals in NF1
    CHANGYAN CHEN; Fiscal Year: 2013
    ..mitotic exit involves an intricately ordered series of events, leading from the splitting of sister chromatids at anaphase onset to the completion of cell division by cytokinesis...
  17. Regulation of Borealin Function by Mitotic Phosphorylation
    William Taylor; Fiscal Year: 2009
    ..preliminary studies indicate that Borealin may be stabilized during mitosis and targeted for degradation by the anaphase promoting complex...
  18. REGULATION OF THE DEVELOPMENT OF TRYPANOSOMA BRUCEI
    CHING CHUNG WANG; Fiscal Year: 2012
    ..in a novel chromosomal passenger complex (CPC) that trans-localizes from the central spindle midzone in late anaphase across the nuclear envelope to the FAZ and then transcends down from the anterior to the posterior end along the ..
  19. Age and molecular mechanisms contributing to aneuploidy in oocytes
    Richard M Schultz; Fiscal Year: 2013
    ..The SAC is one pathway that prevents segregation errors by blocking the onset of anaphase until all chromosomes make proper attachments to the spindle...
  20. Regulation of cell division by mitotic kinases
    MICHAEL ALTERMAN LAMPSON; Fiscal Year: 2012
    ..One of these kinases, Aurora B, localizes to the centromere until anaphase and regulates the attachments of chromosomes to the mitotic spindle...
  21. Studying Mitosis using Xenopus Egg Extracts
    Rebecca W Heald; Fiscal Year: 2013
    ..and kinetochore formation and function, through the use of sperm chromosomes that can duplicate and undergo anaphase segregation in vitro...
  22. Nucleoporins in Chromosomal Instability and Cancer
    Jan M van Deursen; Fiscal Year: 2012
    ..The main mitotic defect that we observe is chromatin-bridge formation in anaphase, a phenotype reminiscent of impaired topoisomerase II[unreadable] function...
  23. Molecular mechanisms underlying chromosomal instability and aneuploidy
    Wei Dai; Fiscal Year: 2012
    ..between sister chromatids during the S, G2, and early mitotic phases of the cell cycle until the onset of anaphase to prevent the adverse consequences of abnormal chromosomal segregation...
  24. The Control of Centriole Duplication and Degeneration
    Meng Fu Bryan Tsou; Fiscal Year: 2013
    ..This has allowed us to determine that the licensing event for centrosome duplication occurs in anaphase, mediated in part by the separase protease...
  25. Molecular Mechanisms of Meiotic Maturation in Drosophila
    Daniela Drummond-Barbosa; Fiscal Year: 2013
    ..promotes the stability of the cell cycle regulators CyclinA, Polo and Twine/Cdc25 (all known to be targets of the Anaphase Promoting Complex, or APC), and that it also has a separate role via inhibition of the E3 ubiquitin ligase Elgi ..
  26. Mechanism controlling centrosome duplication
    Barbara E Tanos; Fiscal Year: 2013
    ..known to work together to remove the cohesin complex from chromosomes to facilitate chromosome segregation during anaphase, which coincides with centriole disengagement...
  27. Mechanistic Analysis Of Microtubule Based Motors
    Susan P Gilbert; Fiscal Year: 2012
    ..specific aims: 1) Define the Kar3Cik1 mechanochemistry for its crosslinking function of anti-parallel MTs at anaphase. 2) Define the Kar3Vik1 mechanochemistry for its accumulation at MT minus-ends to crosslink parallel MTs at the ..
  28. Regulation of Sister-Chromatid Cohesion in Drosophila Meiosis
    SHARON ELIZABETH BICKEL; Fiscal Year: 2013
    ..sister chromatids provides an evolutionarily conserved mechanism to keep recombinant chromosomes associated until anaphase I and thereby ensure their accurate segregation during meiosis I...
  29. Biochemistry of Anaphase Promoting Complex-mediated Ubiquitination
    Mark J Solomon; Fiscal Year: 2013
    ..The initiation of chromosome separation (anaphase) and completion of the cell cycle require the degradation of a number of cell cycle regulatory proteins such as ..
  30. Systems Architecture And Dynamical Behaviors of the Kinases That Drive M-Phase
    JOSEPH RICHARD POMERENING; Fiscal Year: 2013
    ..activities with CDK1, and how they operate as a unit to then modulate mitotic exit through the activity of the anaphase-promoting complex (APC)...
  31. TRANSPOSASE PROTEIN METNASE IN LEUKEMIC DECATENATION
    Robert A Hromas; Fiscal Year: 2011
    ..results in intertwined sister chromatids that must be untangled, or decatenated, before chromatid separation at anaphase. When absent, catastrophic chromatid breakage can occur, and the multiple breaks can result in inter- chromosomal ..
  32. MICROTUBULE REGULATION
    Don W Cleveland; Fiscal Year: 2013
    ..Each unattached kinetochore generates a "wait anaphase" inhibitor that blocks destruction of cyclin B and securin, thereby blocking disjoining of duplicated sister ..
  33. Meiotic Chromosome Segregation in C. elegans
    Anne M Villeneuve; Fiscal Year: 2013
    ..undergo recombination, and reorganize again to reveal chiasmata, structures that hold homologs together until anaphase I and direct orientation of linked homolog pairs (bivalents) on the meiosis I spindle...
  34. A novel mouse colon cancer model and chemoprevention
    Wei Dai; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): The spindle checkpoint delays the progression from metaphase to anaphase until all condensed chromosomes are properly attached to mitotic spindles...
  35. Tubulin-Binding Upconversion Nanoparticles for Breast-Cancer Imaging and Therapy
    Chuanbin Mao; Fiscal Year: 2013
    ..the mitotic spindle functions will be perturbed, resulting in the inhibition of cell division at the metaphase/anaphase transition of mitosis...
  36. REGULATION OF THE DEVELOPMENT OF TRYPANOSOMA BRUCEI
    Ching Wang; Fiscal Year: 2009
    ..in a novel chromosomal passenger complex (CPC) that trans-localizes from the central spindle midzone in late anaphase across the nuclear envelope to the FAZ and then transcends down from the anterior to the posterior end along the ..
  37. Mechanisms of Transient Transcription in Yeast
    RANDY S STRICH; Fiscal Year: 2012
    ..We have recently discovered that early meiotic gene induction requires Ume6p destruction by the Cdc20p- directed anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase...
  38. Roles of chromosomal factors in chromosome segregation
    Hironori Funabiki; Fiscal Year: 2013
    ..At centromeres, the CPC mediates spindle assembly checkpoint (SAC) signaling to delay anaphase onset in response to erroneous kinetochore attachments...
  39. Mechanisms of the Unusual Cytokinesis in Trypanosomes
    Ziyin Li; Fiscal Year: 2013
    ..localization during mitosis and cytokinesis by migrating from chromatins to the central spindle during metaphase-anaphase transition and from the central spindle to the anterior tip of the new FAZ during mitosis-cytokinesis transition (..
  40. Regulation of Chromosome Segregation
    DAVID OWEN MORGAN; Fiscal Year: 2013
    ..During mitosis, the sister-chromatid pairs are oriented on the bipolar mitotic spindle. At the metaphase-anaphase transition, the cohesin linkage between sister's chromatids is abruptly dissolved by a protease called ..
  41. Regulation of the DNA damage response by ATM-MRN pathway
    Jean Gautier; Fiscal Year: 2013
    ..reactive oxygen species, collapse of stalled replication forks, telomere dysfunction, chromosome breakage during anaphase, or following programmed genomic rearrangements during immune and germ cell maturation...
  42. Motor Protein Dynamics and Mitotic Mechanisms
    Tarun M Kapoor; Fiscal Year: 2013
    ..the proper organization of complex and dynamic microtubule-based structures, such as the bipolar spindle prior to anaphase and the central spindle (or midzone) during anaphase...
  43. Retinal Patterning During Eye Development
    Marek Mlodzik; Fiscal Year: 2013
    ..factors identified to act in the R3/R4 Fz/PCP- mediated cell fate specification, and (4) to define the role of anaphase promoting complex (APC/C) subunits and a Ubiquitin-like peptide in Fz/PCP signaling and R3/R4 specification...
  44. Regulation of M phase exit
    SALLY A KORNBLUTH; Fiscal Year: 2010
    ..The destruction of Cyclin B at M phase exit is driven by the APC (anaphase promoting complex), a multi-protein E3 ubiquitin ligase, which ubiquitylates Cyclin B and promotes its ..
  45. CELL CYCLE REGULATION
    MARC WALLACE KIRSCHNER; Fiscal Year: 2012
    ..We also propose to investigate in detail the order in which a given E3 enzyme, particularly the anaphase promoting complex (APC), ubiquitinates its substrates...
  46. Mechanisms of Spindle Assembly
    Claire E Walczak; Fiscal Year: 2012
    ..developed in Aim 2 along with the molecular perturbations outlined in Aim 1 to quantitatively analyze pre-anaphase chromosome movement...
  47. BIOCHEMICAL AND GENETIC ANALYSIS OF YEAST KINETOCHORES
    PETER KARL SORGER; Fiscal Year: 2010
    ..bind paired sister chromatids to spindle microtubules (MTs) in a bipolar fashion compatible with disjunction at anaphase (ii) they move chromosomes back and forth along spindle MTs by coupling plus-end dynamics to chromosome movement ..
  48. The role of establishment factors in cohesion
    Jerard Hurwitz; Fiscal Year: 2010
    ..from the time of their duplication in S-phase until their equal distribution between daughter cells during anaphase. Though sister chromatid pairing and partition to daughter cells are under intensive investigation in many ..
  49. Kinetochore Specification and Function
    Arshad Desai; Fiscal Year: 2013
    ..an important role in the spindle checkpoint-the kinetochore-anchored regulatory pathway that generates a "wait anaphase'signal until all of the chromosomes in a cell are correctly attached to the spindle...
  50. Neuronal CDh1 Regulation and Involvement in Synaptic Plasticity and Memory
    Joseph E Pick; Fiscal Year: 2011
    ..It affects about 5 million Americans, and there is no known cure. Cdh1 is a protein that activates the anaphase promoting complex (APC) and binds specific proteins so that they can be marked for degradation...
  51. Stable Maintenance of an Extrachromosomal Selfish DNA Element
    Makkuni Jayaram; Fiscal Year: 2010
    ..plasmid segregation fails if the plasmid does not acquire cohesin concomitant with DNA replication or if the anaphase disassembly of cohesin is blocked...
  52. Regulation and function of the Clp 1p protein phosphatase
    Dannel McCollum; Fiscal Year: 2010
    ..3) To test a hypothesis that Clp1 promotes actomyosin ring stability through effects on Cdc15. 4) To determine how Clp1 and the anaphase promoting complex promote Cdc25 inactivation in late mitosis.
  53. CELL AND TUMOR GENETICS OF MITOTIC ARREST CHECKPOINTS
    PETER KARL SORGER; Fiscal Year: 2010
    ..and transmitted via a transduction system comprised of Mad and Bub proteins, act to regulate the activity of the anaphase promoting complex (APC) thereby linking cell cycle progression to the correct execution of the mechanical events ..
  54. KINETOCHORE STRUCTURE AND FUNCTION
    TIMOTHY YEN; Fiscal Year: 2010
    ..Our discovery of the Mitotic Checkpoint Complex (MCC) as the major inhibitor of the Anaphase Promoting Complex (APC) in Hela cells led to a new two-step model for how APC is inhibited by the checkpoint...
  55. Disabling of the Anaphase Promoting Complex by Human Cytomegalovirus
    DEBORAH HYE SPECTOR; Fiscal Year: 2010
    ..A specific target of the virus is the multisubunit Anaphase Promoting Complex (APC) E3 ubiquitin ligase...
  56. Conservation of allosteric mechanisms within an enzyme family
    ADAM STEEVES; Fiscal Year: 2012
    ..The formation of K48-linked polyubiquitin chains by the anaphase promoting complex (APC/C) in the ubiquitin-proteasome pathway requires the sequential action of two E2s, Ubc1 and ..
  57. MOLECULAR CONTROL OF CELL PROLIFERATION
    DAVID OWEN MORGAN; Fiscal Year: 2013
    ..This process is triggered by a poorly understood enzyme called the Anaphase-Promoting Complex or APC...
  58. The Micromechanics of Central Spindle Organization
    SCOTT THOMAS FORTH; Fiscal Year: 2013
    ..of microtubules which adopts a bipolar configuration in metaphase and a central spindle upon entry into anaphase. This structure is subjected to numerous forces throughout mitosis, and must provide stability while remaining ..
  59. ATP-Dependent Chromatin Remodeling in Human Malignancy
    Gerald R Crabtree; Fiscal Year: 2013
    ..We have found that conditional deletion or depletion of the oncogenic subunits leads to stalling in mitosis and anaphase bridge formation, strongly implicating a failure to decatenate DNA during M phase...
  60. Regulation of Cellular Growth and Division by Ubiquitin-Mediated Proteolysis
    Jennifer A Benanti; Fiscal Year: 2012
    ..Two ubiquitin ligase complexes that are known to regulate the cell cycle are the Anaphase Promoting Complex (APC) and Skp1-Cul1-F-box (SCF) complex...
  61. Mechanism of mitotic exit in budding yeast
    Raymond Deshaies; Fiscal Year: 2009
    ..During anaphase-telophase, the protein phosphatase Cdc14 enables the exit from mitosis by reversing these inhibitory ..
  62. FORMATION,INTERACTION & FUNCTION OF SPINDLE COMPONENTS
    Conly L Rieder; Fiscal Year: 2010
    ..associated motor responsiblefor fast poleward motion during kinetochore attachment is no longer functional during anaphase, and b) that ZW-10 is involved in kinetochore re-orientation during mitosis &meiosis in Drosophila...
  63. ID2 IN CELL CYCLE REGULATION AND CANCER
    Antonio Iavarone; Fiscal Year: 2010
    ..we discovered that Id2 is a highly unstable protein that is targeted for ubiquitin-dependent degradation by the Anaphase Promoting Complex/Cyclosome (APC/C) and its activator Cdh1...
  64. MEIOTIC CHROMOSOME SEGREGATION IN YEAST
    Dean Dawson; Fiscal Year: 1992
    ..between the homologous chromosomes ensure correct meiosis I segregation by holding the pair together until anaphase I when they move apart...
  65. S POMBE PROTEIN/CENTROMERIC DNA INTERACTION
    Jerard Hurwitz; Fiscal Year: 2001
    ..and it is the last site by which sister chromatids remain associated prior to their separation at the metaphase-anaphase transition...
  66. Control of Genomic Stability by Emi1 and Securin
    NORMAN LEHMAN; Fiscal Year: 2007
    ..is a protein that functions to ensure accurate distribution of chromosomes to daughter cells by inhibiting anaphase progression until all chromosomes are properly aligned at metaphase...
  67. BIMA AND MITOSIS
    PETER MIRABITO; Fiscal Year: 1999
    The metaphase to anaphase transition is a complex and critical step in mitosis...