energy transfer

Summary

Summary: The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed). In biochemistry, this concept generally refers to the transfer of groups from compounds that contain energy-rich bonding arrangements to compounds that have relatively energy-poor bonding characteristics via thermodynamically permissible enzymatic reactions. This principle is a major premise of the interaction between energy-producing and energy-utilizing metabolic pathways in living cells. (From Devlin, Textbook of biochemistry with clinical correlations, 3d ed, p246)

Top Publications

  1. ncbi Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin
    A Miyawaki
    Department of Pharmacology, University of California, San Diego, La Jolla 92093 0647, USA
    Nature 388:882-7. 1997
  2. ncbi Recent advances in bioluminescence resonance energy transfer technologies to study GPCR heteromerization
    Mohammed A Ayoub
    Western Australian Institute for Medical Research WAIMR, University of Western Australia, Nedlands, Perth, Western Australia, Australia
    Curr Opin Pharmacol 10:44-52. 2010
  3. pmc Activation of rac and cdc42 video imaged by fluorescent resonance energy transfer-based single-molecule probes in the membrane of living cells
    Reina E Itoh
    Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University, CREST, Japan
    Mol Cell Biol 22:6582-91. 2002
  4. ncbi Quantum coherent energy transfer over varying pathways in single light-harvesting complexes
    Richard Hildner
    ICFO Institut de Ciencies Fotoniques, Mediterranean Technology Park, Castelldefels, Barcelona, Spain
    Science 340:1448-51. 2013
  5. ncbi Environment-assisted quantum walks in photosynthetic energy transfer
    Masoud Mohseni
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St, Cambridge, Massachusetts 02138, USA
    J Chem Phys 129:174106. 2008
  6. ncbi Fluorescence resonance energy transfer microscopy of localized protein interactions in the living cell nucleus
    R N Day
    Department of Medicine, NSF Center for Biological Timing, Charlottesville, Virginia 22908, USA
    Methods 25:4-18. 2001
  7. ncbi Single-molecule fluorescence resonance energy transfer
    T Ha
    Department of Physics, Center for Biophysics and Computational Biology, Urbana 61801, USA
    Methods 25:78-86. 2001
  8. pmc Biosensing and imaging based on bioluminescence resonance energy transfer
    Zuyong Xia
    Department of Radiology and Bio X Program, Stanford University School of Medicine, Stanford, CA 94305 5484, USA
    Curr Opin Biotechnol 20:37-44. 2009
  9. ncbi High-throughput screening of G protein-coupled receptor antagonists using a bioluminescence resonance energy transfer 1-based beta-arrestin2 recruitment assay
    Fadi F Hamdan
    University of Montreal, Department of Biochemistry, Montreal, Quebec, Canada
    J Biomol Screen 10:463-75. 2005
  10. pmc Fluorescence resonance energy transfer-based stoichiometry in living cells
    Adam Hoppe
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Biophys J 83:3652-64. 2002

Detail Information

Publications385 found, 100 shown here

  1. ncbi Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin
    A Miyawaki
    Department of Pharmacology, University of California, San Diego, La Jolla 92093 0647, USA
    Nature 388:882-7. 1997
    ..Binding of Ca2+ makes calmodulin wrap around the M13 domain, increasing the fluorescence resonance energy transfer (FRET) between the flanking GFPs...
  2. ncbi Recent advances in bioluminescence resonance energy transfer technologies to study GPCR heteromerization
    Mohammed A Ayoub
    Western Australian Institute for Medical Research WAIMR, University of Western Australia, Nedlands, Perth, Western Australia, Australia
    Curr Opin Pharmacol 10:44-52. 2010
    ..The bioluminescence resonance energy transfer (BRET) technology has been intimately associated with the study of GPCR-GPCR interactions for the past ten ..
  3. pmc Activation of rac and cdc42 video imaged by fluorescent resonance energy transfer-based single-molecule probes in the membrane of living cells
    Reina E Itoh
    Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University, CREST, Japan
    Mol Cell Biol 22:6582-91. 2002
    ..We developed fluorescent resonance energy transfer-based probes which monitored the local balance between the activities of guanine nucleotide exchange factors ..
  4. ncbi Quantum coherent energy transfer over varying pathways in single light-harvesting complexes
    Richard Hildner
    ICFO Institut de Ciencies Fotoniques, Mediterranean Technology Park, Castelldefels, Barcelona, Spain
    Science 340:1448-51. 2013
    ..Here, we demonstrate ultrafast quantum coherent energy transfer within individual antenna complexes of a purple bacterium under physiological conditions...
  5. ncbi Environment-assisted quantum walks in photosynthetic energy transfer
    Masoud Mohseni
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St, Cambridge, Massachusetts 02138, USA
    J Chem Phys 129:174106. 2008
    b>Energy transfer within photosynthetic systems can display quantum effects such as delocalized excitonic transport...
  6. ncbi Fluorescence resonance energy transfer microscopy of localized protein interactions in the living cell nucleus
    R N Day
    Department of Medicine, NSF Center for Biological Timing, Charlottesville, Virginia 22908, USA
    Methods 25:4-18. 2001
    ..can be achieved with the conventional light microscope using the technique of fluorescence resonance energy transfer (FRET)...
  7. ncbi Single-molecule fluorescence resonance energy transfer
    T Ha
    Department of Physics, Center for Biophysics and Computational Biology, Urbana 61801, USA
    Methods 25:78-86. 2001
    Fluorescent resonance energy transfer (FRET) is a powerful technique for studying conformational distribution and dynamics of biological molecules...
  8. pmc Biosensing and imaging based on bioluminescence resonance energy transfer
    Zuyong Xia
    Department of Radiology and Bio X Program, Stanford University School of Medicine, Stanford, CA 94305 5484, USA
    Curr Opin Biotechnol 20:37-44. 2009
    Bioluminescence resonance energy transfer (BRET) operates with biochemical energy generated by bioluminescent proteins to excite fluorophores and offers additional advantages over fluorescence energy transfer (FRET) for in vivo imaging ..
  9. ncbi High-throughput screening of G protein-coupled receptor antagonists using a bioluminescence resonance energy transfer 1-based beta-arrestin2 recruitment assay
    Fadi F Hamdan
    University of Montreal, Department of Biochemistry, Montreal, Quebec, Canada
    J Biomol Screen 10:463-75. 2005
    ..This allowed the use of a bioluminescence resonance energy transfer (BRET) 1- beta-arrestin2 translocation assay to quantify receptor activation or inhibition...
  10. pmc Fluorescence resonance energy transfer-based stoichiometry in living cells
    Adam Hoppe
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Biophys J 83:3652-64. 2002
    Imaging of fluorescence resonance energy transfer (FRET) between fluorescently labeled molecules can measure the timing and location of intermolecular interactions inside living cells...
  11. ncbi Primary photophysical processes in photosystem II: bridging the gap between crystal structure and optical spectra
    Thomas Renger
    Institut fur Theoretische Physik, Johannes Kepler Universitat, Altenberger Strasse 69, 4040 Linz, Austria
    Chemphyschem 11:1141-53. 2010
    ..II (PS-II) and how these properties are related to the photosynthetic function, that is, excitation energy transfer from the antenna complexes to the reaction center (RC) and the subsequent transmembrane charge separation in ..
  12. ncbi The architecture and function of the light-harvesting apparatus of purple bacteria: from single molecules to in vivo membranes
    Richard J Cogdell
    Biomedical Research Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
    Q Rev Biophys 39:227-324. 2006
    ..The elegance of the purple bacterial light-harvesting system lies in the way in which it has cleverly exploited quantum mechanics...
  13. ncbi Monitoring the activation state of the insulin-like growth factor-1 receptor and its interaction with protein tyrosine phosphatase 1B using bioluminescence resonance energy transfer
    Christophe Blanquart
    Department of Cell Biology, Institut Cochin, 22 rue Mechain, 75014 Paris, France
    Mol Pharmacol 68:885-94. 2005
    We have developed two bioluminescence resonance energy transfer (BRET)-based approaches to monitor 1) ligand-induced conformational changes within partially purified insulin-like growth factor-1 (IGF-1) receptors (IGF1R) and 2) IGF1R ..
  14. pmc Altered energy transfer from mitochondria to sarcoplasmic reticulum after cytoarchitectural perturbations in mice hearts
    James R Wilding
    U 769 INSERM, Chatenay Malabry, France
    J Physiol 575:191-200. 2006
    ..Changes in cytoarchitecture may therefore impair subcellular energy transfer and contribute to energetic and contractile dysfunction.
  15. pmc Energy transfer in photosystem I of cyanobacteria Synechococcus elongatus: model study with structure-based semi-empirical Hamiltonian and experimental spectral density
    Mino Yang
    Department of Chemistry, University of California, Berkeley, California, USA
    Biophys J 85:140-58. 2003
    We model the energy transfer and trapping kinetics in PSI. Rather than simply applying Förster theory, we develop a new approach to self-consistently describe energy transfer in a complex with heterogeneous couplings...
  16. pmc Excitation energy transfer and trapping in higher plant Photosystem II complexes with different antenna sizes
    Stefano Caffarri
    Aix Marseille Universite, Laboratoire de Genetique et Biophysique des Plantes, Marseille, France
    Biophys J 100:2094-103. 2011
    ..01% α-DM. Excitation energy transfer and trapping were investigated by coarse-grained modeling of the fluorescence kinetics...
  17. ncbi Plasmon-mediated radiative energy transfer across a silver nanowire array via resonant transmission and subwavelength imaging
    Zhang Kai Zhou
    Department of Physics, Key Laboratory of Acoustic and Photonic Materials and Devices of Ministry of Education, Wuhan University, Wuhan 430072, People s Republic of China
    ACS Nano 4:5003-10. 2010
    Efficient plasmon-mediated excitation energy transfer between the CdSe/ZnS semiconductor quantum dots (QDs) across the silver nanowire array up to 560 nm in length is observed...
  18. pmc Femtosecond dynamics of rubredoxin: tryptophan solvation and resonance energy transfer in the protein
    Dongping Zhong
    Laboratory for Molecular Sciences, Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 99:13-8. 2002
    ..We also report results on energy transfer from Trp to the iron-sulfur [Fe-S] cluster...
  19. ncbi A homogenous assay to monitor the activity of the insulin receptor using Bioluminescence Resonance Energy Transfer
    Tarik Issad
    Department of Cell Biology, Cochin Institute, CNRS, INSERM, University Rene Descartes, 22 rue Mechain, 75014 Paris, France
    Biochem Pharmacol 64:813-7. 2002
    ..We have developed a procedure based on Bioluminescence Resonance Energy Transfer (BRET) to monitor the activation state of the insulin receptor...
  20. ncbi Resonance energy transfer from a dye molecule to graphene
    R S Swathi
    Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore 560012, India
    J Chem Phys 129:054703. 2008
    We study the distance dependence of the rate of resonance energy transfer from the excited state of a dye to the pi system of graphene...
  21. ncbi The experimental survey of protein-folding energy landscapes
    Mikael Oliveberg
    Department of Biochemistry and Biophysics, Arrhenius Laboratories of Natural Sciences, Stockholm University, Stockholm, Sweden
    Q Rev Biophys 38:245-88. 2005
    ....
  22. ncbi Terbium and rhodamine as labels in a homogeneous time-resolved fluorometric energy transfer assay of the beta subunit of human chorionic gonadotropin in serum
    K Blomberg
    Wallac Oy, Turku, Finland
    Clin Chem 45:855-61. 1999
    Fluorescence resonance energy transfer (FRET) is a powerful tool in analytical chemistry. The aim of the present work was to use FRET to design a homogeneous immunoassay.
  23. pmc Distribution of a glycosylphosphatidylinositol-anchored protein at the apical surface of MDCK cells examined at a resolution of <100 A using imaging fluorescence resonance energy transfer
    A K Kenworthy
    Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Cell Biol 142:69-84. 1998
    ..proteins in intact cell membranes, we used a novel form of digital microscopy, imaging fluorescence resonance energy transfer (FRET), which extends the resolution of fluorescence microscopy to the molecular level (<100 A)...
  24. ncbi Arrangement of apolipoprotein A-I in reconstituted high-density lipoprotein disks: an alternative model based on fluorescence resonance energy transfer experiments
    M A Tricerri
    Department of Biochemistry, Laboratory for Fluorescence Dynamics, and Department of Mathematics, University of Illinois at Urbana-Champaign, 61801, USA
    Biochemistry 40:5065-74. 2001
    ..and dipalmitoylphosphatidylcholine, and the complexes were used in three quantitative fluorescence resonance energy transfer (FRET) experiments to determine the distances between two specific sites in an HDL particle...
  25. pmc Cerenkov radiation energy transfer (CRET) imaging: a novel method for optical imaging of PET isotopes in biological systems
    Robin S Dothager
    BRIGHT Institute, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS ONE 5:e13300. 2010
    ..Upon β(+) decay, the initial velocity of high-energy β(+) particles can momentarily exceed the speed of light in tissue, producing Cerenkov radiation that is detectable by optical imaging, but is highly absorbed in living organisms...
  26. ncbi Quantum dot-based energy transfer: perspectives and potential for applications in photodynamic therapy
    Anna C S Samia
    Center for Chemical Dynamics and Nanomaterials Research, Department of Chemistry, Case Western Reserve University, Cleveland, OH, USA
    Photochem Photobiol 82:617-25. 2006
    ..The photophysics of singlet oxygen generation in relation to quantum dot-based energy transfer is discussed and the possibility of using quantum dots as photosensitizer in PDT is assessed, including their ..
  27. ncbi Extended bioluminescence resonance energy transfer (eBRET) for monitoring prolonged protein-protein interactions in live cells
    Kevin D G Pfleger
    7TM Laboratory Laboratory for Molecular Endocrinology, Western Australian Institute for Medical Research WAIMR and Centre for Medical Research, University of Western Australia, Nedlands, Perth, WA 6009, Australia
    Cell Signal 18:1664-70. 2006
    Bioluminescence resonance energy transfer (BRET) is an increasingly popular technique for studying protein-protein interactions in live cells...
  28. pmc Antisense inhibition of the photosynthetic antenna proteins CP29 and CP26: implications for the mechanism of protective energy dissipation
    J Andersson
    Umea Plant Science Centre, Department of Plant Physiology, Umea University, S 901 87 Umea, Sweden
    Plant Cell 13:1193-204. 2001
    ..High-light-induced violaxanthin deepoxidation to zeaxanthin was not affected, although the pool size of these pigments was decreased slightly. We conclude that CP29 and CP26 are unlikely to be sites for nonphotochemical quenching...
  29. pmc Fluorescence resonance energy transfer over approximately 130 basepairs in hyperstable lac repressor-DNA loops
    Laurence M Edelman
    Department of Chemistry and Biochemistry, University of Maryland, College Park, College Park, Maryland 20742 2021, USA
    Biophys J 84:1131-45. 2003
    ..Here, fluorescence resonance energy transfer (FRET) is measured on DNA loops, between fluorescein and TAMRA attached near the two operators, approximately ..
  30. pmc An improved bioluminescence resonance energy transfer strategy for imaging intracellular events in single cells and living subjects
    Abhijit De
    Molecular Imaging Program at Stanford and Bio X Program, Department of Radiology, School of Medicine, Stanford University, Stanford, California 94305, USA
    Cancer Res 67:7175-83. 2007
    Bioluminescence resonance energy transfer (BRET) is currently used for monitoring various intracellular events, including protein-protein interactions, in normal and aberrant signal transduction pathways...
  31. ncbi Adenosine A2A-dopamine D2 receptor-receptor heteromerization: qualitative and quantitative assessment by fluorescence and bioluminescence energy transfer
    Meritxell Canals
    Department of Biochemistry and Molecular Biology, University of Barcelona, Martí y Franquès 1, E 08028 Barcelona, Spain
    J Biol Chem 278:46741-9. 2003
    ..In the present work, fluorescence resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET) techniques were used to confirm the occurrence of ..
  32. ncbi Homodimerization of the beta2-adrenergic receptor as a prerequisite for cell surface targeting
    Ali Salahpour
    Department of Biochemistry and Groupe de Recherche sur le Système Nerveux Autonome, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    J Biol Chem 279:33390-7. 2004
    ..Taken together, these data indicate that beta2AR homodimerization plays an important role in ER export and cell surface targeting...
  33. ncbi Characterization of IRA/IRB hybrid insulin receptors using bioluminescence resonance energy transfer
    Christophe Blanquart
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    Biochem Pharmacol 76:873-83. 2008
    ....
  34. ncbi Interfacial water facilitates energy transfer by inducing extended vibrations in membrane lipids
    Alireza Mashaghi
    FOM Institute AMOLF, Science Park 104, 1098XG Amsterdam, The Netherlands
    J Phys Chem B 116:6455-60. 2012
    ..We find that water hydrating the lipid headgroups enables efficient energy transfer across membrane leaflets on sub-picosecond time scales...
  35. ncbi Fluorescence resonance energy transfer as a probe for G-quartet formation by a telomeric repeat
    J L Mergny
    Laboratoire de Biophysique, Museum national d histoire naturelle, INSERM U 201, CNRS UMR 8646, 43 rue Cuvier, 75005 Paris, France
    Chembiochem 2:124-32. 2001
    ..Intramolecular folding of a telomeric oligonucleotide into a quadruplex led to fluorescence resonance energy transfer (FRET) between a donor (fluorescein) and an acceptor (tetramethylrhodamine) covalently attached to the 5' and ..
  36. ncbi Structural basis for efficient chromophore communication and energy transfer in a constructed didomain protein scaffold
    James A J Arpino
    School of Biosciences, Main Building, Park Place, Cardiff University, Cardiff CF10 3AT, United Kingdom
    J Am Chem Soc 134:13632-40. 2012
    ..A new light-capturing ultra-high-efficiency energy transfer protein scaffold has been constructed by coupling the chromophore centers of two normally unrelated proteins: ..
  37. pmc Electronic resonance with anticorrelated pigment vibrations drives photosynthetic energy transfer outside the adiabatic framework
    Vivek Tiwari
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309 0215, USA
    Proc Natl Acad Sci U S A 110:1203-8. 2013
    The delocalized, anticorrelated component of pigment vibrations can drive nonadiabatic electronic energy transfer in photosynthetic light-harvesting antennas...
  38. pmc Electrochemiluminescence energy transfer-promoted ultrasensitive immunoassay using near-infrared-emitting CdSeTe/CdS/ZnS quantum dots and gold nanorods
    Lingling Li
    State Key Laboratory of Analytical Chemistry for Life Science, Synergetic Innovation Center of Chemical Nature of Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P R China
    Sci Rep 3:1529. 2013
    The marriage of energy transfer with electrochemiluminescence has produced a new technology named electrochemiluminescence energy transfer (ECL-ET), which can realize effective and sensitive detection of biomolecules...
  39. ncbi Colocalization and fluorescence resonance energy transfer between cdk5 and AT8 suggests a close association in pre-neurofibrillary tangles and neurofibrillary tangles
    Jean C Augustinack
    Department of Neurology, Harvard Medical School, Massachusetts General Hospital, Charlestown 02129, USA
    J Neuropathol Exp Neurol 61:557-64. 2002
    ..We further examined colocalization using fluorescence resonance energy transfer. This suggests a close, stable intermolecular association between cdk5 and phosphorylated tau, consistent ..
  40. ncbi Characterization of one- and two-photon excitation fluorescence resonance energy transfer microscopy
    Masilamani Elangovan
    W M Keck Center for Cellular Imaging, Gilmer Hall, 22904, Charlottesville, VA, USA
    Methods 29:58-73. 2003
    ..protein inside the living cell became possible through the application of advanced fluorescence resonance energy transfer (FRET) microscope techniques...
  41. ncbi One- and two-photon fluorescence resonance energy transfer microscopy to establish a clustered distribution of receptor-ligand complexes in endocytic membranes
    Horst Wallrabe
    University of Virginia, Department of Biology, Gilmer Hall, 057, Charlottesville, Virginia 22904, USA
    J Biomed Opt 8:339-46. 2003
    One- and two-photon fluorescence resonance energy transfer (FRET) microscopy, using different bandwidth emission filters and a novel spectral spillover correction algorithm (PFRET algorithm), provides the basis for a quantitative ..
  42. pmc Bridging the gap between structural and lattice models: a parameterization of energy transfer and trapping in Photosystem I
    Bas Gobets
    Division of Physics and Astronomy of the Faculty of Exact Sciences and Institute of Molecular Biological Sciences, Vrije Universiteit, Amsterdam, The Netherlands
    Biophys J 85:3872-82. 2003
    ..the validity and other potential merits of such an approach we present the results of the modeling of the energy transfer and trapping in Photosystem I based upon the 2...
  43. ncbi DNA bending induced by high mobility group proteins studied by fluorescence resonance energy transfer
    M Lorenz
    Institute for Molecular Biotechnology, Jena, Germany
    Biochemistry 38:12150-8. 1999
    ..DNA binding also induces a substantial bend. Using fluorescence resonance energy transfer (FRET), we have determined the changes in the end-to-end distance consequent on the binding of selected ..
  44. ncbi Efficient energy transfer within self-assembling peptide fibers: a route to light-harvesting nanomaterials
    Kevin J Channon
    Cavendish Laboratory, University of Cambridge, Cambridge CB3 0HE, UK
    J Am Chem Soc 131:12520-1. 2009
    ..We use fluorescence spectroscopy to demonstrate that the resulting complex performs a light-harvesting function...
  45. ncbi Elevated ΔpH restores rapidly reversible photoprotective energy dissipation in Arabidopsis chloroplasts deficient in lutein and xanthophyll cycle activity
    Matthew P Johnson
    School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, Fogg Building, London, E1 4NS, UK
    Planta 235:193-204. 2012
    ....
  46. ncbi Transversal and lateral exciton energy transfer in grana thylakoids of spinach
    Helmut Kirchhoff
    Institut fur Botanik, Schlossgarten 3, Wilhelm Klemm Strasse 10, D 48149 Munster, Germany
    Biochemistry 43:14508-16. 2004
    The excitation energy transfer between photosystem (PS) II complexes was studied in isolated grana disks and thylakoids using chlorophyll a fluorescence induction measurements in the presence of DCMU under stacked and destacked ..
  47. ncbi Site, rate, and mechanism of photoprotective quenching in cyanobacteria
    Lijin Tian
    Laboratory of Biophysics, Wageningen University, P O Box 8128, 6700 ET, Wageningen, The Netherlands
    J Am Chem Soc 133:18304-11. 2011
    ..pigment pools in the phycobilisomes and photosystems I and II, and to determine the rates of excitation energy transfer between them...
  48. pmc Mapping of the localization of type 1 angiotensin receptor in membrane microdomains using bioluminescence resonance energy transfer-based sensors
    Andras Balla
    Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary
    J Biol Chem 287:9090-9. 2012
    ..movement between plasma membrane compartments in response to stimulation using bioluminescence resonance energy transfer measurements...
  49. ncbi Light-harvesting and structural organization of Photosystem II: from individual complexes to thylakoid membrane
    Roberta Croce
    Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Photochem Photobiol B 104:142-53. 2011
    ..of pigment-protein complexes in PSII and relate this to experimental and theoretical results on excitation energy transfer (EET) and charge separation (CS)...
  50. ncbi Fluorescence resonance energy transfer imaging microscopy and fluorescence polarization imaging microscopy
    Yuling Yan
    Department of Mechanical Engineering, University of Hawaii at Manoa, Honolulu 96822, USA
    Methods Enzymol 360:561-80. 2003
  51. ncbi Self-assembled DNA photonic wire for long-range energy transfer
    Jonas K Hannestad
    Department of Chemical and Biological Engineering Physical Chemistry, Chalmers University of Technology, SE 41296 Gothenburg, Sweden
    J Am Chem Soc 130:15889-95. 2008
    ..a scaffold for a chromophore with overlapping absorption and emission bands enabling fluorescence resonance energy transfer (FRET) between pairs of chromophores leading to sequential transfer of the excitation energy along the wire...
  52. pmc Xanthorhodopsin: a proton pump with a light-harvesting carotenoid antenna
    Sergei P Balashov
    University of California, Irvine, CA 92697, USA
    Science 309:2061-4. 2005
    b>Energy transfer from light-harvesting carotenoids to chlorophyll is common in photosynthesis, but such antenna pigments have not been observed in retinal-based ion pumps and photoreceptors...
  53. pmc The Arabidopsis repressor of light signaling, COP1, is regulated by nuclear exclusion: mutational analysis by bioluminescence resonance energy transfer
    Chitra Subramanian
    Department of Botany, University of Tennessee, Knoxville, TN 37996 1100, USA
    Proc Natl Acad Sci U S A 101:6798-802. 2004
    Bioluminescence resonance energy transfer (BRET) between Renilla luciferase and yellow fluorescent protein has been adapted to serve as a real-time reporter on protein-protein interactions in live plant cells by using the Arabidopsis ..
  54. ncbi Protein-protein interactions monitored in cells from transgenic mice using bioluminescence resonance energy transfer
    Martin Audet
    Department of Biochemistry, Institute for Research in Immunology and Cancer, and Groupe de Recherche Universitaire sur le Médicament, Universite de Montreal, Montreal, Quebec, Canada
    FASEB J 24:2829-38. 2010
    ..Resonance energy transfer approaches represent a promising avenue to directly probe these interactions in real time...
  55. ncbi Conjugated polymer/porphyrin complexes for efficient energy transfer and improving light-activated antibacterial activity
    Chengfen Xing
    Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, People s Republic of China
    J Am Chem Soc 131:13117-24. 2009
    ..They can form a complex through electrostatic interactions, and efficient energy transfer from PTP to TPPN occurs upon irradiation under white light (400-800 nm)...
  56. ncbi Nucleic acid-templated energy transfer leading to a photorelease reaction and its application to a system displaying a nonlinear response
    Manuel Röthlingshöfer
    Institut de Science et Ingénierie Supramoléculaires UMR 7006, Universite de Strasbourg CNRS, 8 allee Gaspard Monge, F67000 Strasbourg, France
    J Am Chem Soc 133:18110-3. 2011
    ..photocleavage of a nitrobenzyl-type linker (NPPOC) at 405 nm wavelength was enabled by nucleic acid-templated energy transfer from a sensitizer (thioxanthenone) to the linker...
  57. ncbi Energy transfer-based multiplexed assay of proteases by using gold nanoparticle and quantum dot conjugates on a surface
    Young Pil Kim
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology KAIST, Daejeon 305 701, Republic of Korea
    Anal Chem 80:4634-41. 2008
    ..We developed a multiplexed assay system of proteases and their inhibition by measuring the energy transfer between quantum dots (QDs) and gold nanoparticles (AuNPs) on a glass slide...
  58. ncbi Quantized plasmon quenching dips nanospectroscopy via plasmon resonance energy transfer
    Gang Logan Liu
    Biomolecular Nanotechnology Center, Berkeley Sensor and Actuator Center, Department of Bioengineering, University of California Berkeley, 442 Stanley Hall, Berkeley, California 94720, USA
    Nat Methods 4:1015-7. 2007
    We observed quantized plasmon quenching dips in resonant Rayleigh scattering spectra by plasmon resonance energy transfer (PRET) from a single nanoplasmonic particle to adsorbed biomolecules...
  59. pmc Crystallographic structure of xanthorhodopsin, the light-driven proton pump with a dual chromophore
    Hartmut Luecke
    Departments of Molecular Biology and Biochemistry, and Physiology and Biophysics, and Center for Biomembrane Systems, University of California, Irvine, CA 92697
    Proc Natl Acad Sci U S A 105:16561-5. 2008
    ..The close approach of the 2 polyenes at their ring ends explains why the efficiency of the excited-state energy transfer is as high as approximately 45%, and the 46 degrees angle between them suggests that the chromophore location ..
  60. ncbi Noninvasive imaging of protein-protein interactions from live cells and living subjects using bioluminescence resonance energy transfer
    Abhijit De
    Molecular Imaging Program at Stanford MIPS and Bio X Program, Department of Radiology, School of Medicine, Stanford University, Stanford, California, USA
    FASEB J 19:2017-9. 2005
    ..advancement of imaging of a distance-dependent physical process, known as the bioluminescent resonance energy transfer (BRET2) signal in living subjects, by using a cooled charge-coupled device (CCD) camera...
  61. ncbi Quantum coherence and its interplay with protein environments in photosynthetic electronic energy transfer
    Akihito Ishizaki
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Phys Chem Chem Phys 12:7319-37. 2010
    Recent experiments suggest that electronic energy transfer in photosynthetic pigment-protein complexes involves long-lived quantum coherence among electronic excitations of pigments. [Engel et al., Nature, 2007, 446, 782-786...
  62. ncbi Win some, lose some: enthalpy-entropy compensation in weak intermolecular interactions
    J D Dunitz
    Organic Chemistry Laboratory, Swiss Federal Institute of Technology, Zurich, Switzerland
    Chem Biol 2:709-12. 1995
    ....
  63. ncbi Remote energy storage in Ni(OH)2 with TiO2 photocatalyst
    Yukina Takahashi
    Institute of Industrial Science, University of Tokyo, Komaba, Tokyo, 153 8505, Japan
    Phys Chem Chem Phys 8:2716-9. 2006
    ..It was revealed that the stored energy could be taken out and used chemically or electrochemically. The oxidized Ni(OH)2 was reduced by gaseous formaldehyde, formate, ethanol and H2O2...
  64. pmc A genetically encoded IL-1β bioluminescence resonance energy transfer sensor to monitor inflammasome activity
    Vincent Compan
    Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    J Immunol 189:2131-7. 2012
    ..Using this sensor, to our knowledge, we describe for the first time the kinetic of the inflammasome activity in living macrophages. With this new probe, we also demonstrated that the pro-IL-1β processing occurs all over the cytoplasm...
  65. ncbi Flexible solid-state supercapacitors based on carbon nanoparticles/MnO2 nanorods hybrid structure
    Longyan Yuan
    Wuhan National Laboratory for Optoelectronics WNLO, and College of Optoelectronic Science and Engineering, Huazhong University of Science and Technology HUST, Wuhan, 430074, China
    ACS Nano 6:656-61. 2012
    ..8 Wh/kg at a power density of 14 kW/kg, and a demonstration of a practical device is also presented, highlighting the path for its enormous potential in energy management...
  66. ncbi Mn²+-bonded reduced graphene oxide with strong radiative recombination in broad visible range caused by resonant energy transfer
    Z X Gan
    National Laboratory of Solid State Microstructures and Department of Physics, Nanjing University, Nanjing 210093, People s Republic of China
    Nano Lett 11:3951-6. 2011
    ..The PL spectra excited by different wavelengths are analyzed to elucidate the mechanism, and the resonant energy transfer between Mn(2+) and sp(2) clusters of the rGO appears to be responsible for the enhanced long-wavelength ..
  67. ncbi Energy transfer, volumetric expansion, and removal of oral biofilms by non-contact brushing
    H J Busscher
    Department of Biomedical Engineering, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    Eur J Oral Sci 118:177-82. 2010
    Non-contact removal of oral biofilms offers advantages beyond the reach of bristles, but it is unknown how energy transfer for removal from brush-to-biofilm occurs...
  68. pmc Quantitative comparison of villin headpiece subdomain simulations and triplet-triplet energy transfer experiments
    Kyle A Beauchamp
    Biochemistry, Physics, and Chemistry Departments, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 108:12734-9. 2011
    ..First, we present and validate a novel method for the quantitative prediction of triplet-triplet energy transfer experiments. Second, we construct a many-state model for HP35 that is consistent with previous experiments...
  69. pmc A close association of torsinA and alpha-synuclein in Lewy bodies: a fluorescence resonance energy transfer study
    N Sharma
    Department of Neurology, Alzheimer s Disease Research Unit, and the Department of Neurology, Molecular Neurogenetics Unit, Massachusetts General Hospital East, Charlestown, Massachusetts Albert Einstein College of Medicine, Bronx, New York
    Am J Pathol 159:339-44. 2001
    ..Using sensitive fluorescent resonance energy transfer (FRET) techniques, we find evidence of a close association between torsinA and alpha-synuclein in Lewy bodies.
  70. pmc Light, nutrients, and food-chain length constrain planktonic energy transfer efficiency across multiple trophic levels
    Elizabeth M Dickman
    Department of Zoology, Miami University, Oxford, OH 45056, USA
    Proc Natl Acad Sci U S A 105:18408-12. 2008
    The efficiency of energy transfer through food chains [food chain efficiency (FCE)] is an important ecosystem function...
  71. ncbi Demonstration of improvements to the bioluminescence resonance energy transfer (BRET) technology for the monitoring of G protein-coupled receptors in live cells
    Martina Kocan
    Laboratory for Molecular Endocrinology GPCRs, Western Australian Institute for Medical Research WAIMR, Centre for Medical Research, University of Western Australia, Perth, Western Australia, Australia
    J Biomol Screen 13:888-98. 2008
    The bioluminescence resonance energy transfer (BRET) technique has become extremely popular for studying protein-protein interactions in living cells and real time...
  72. ncbi Two-photon absorbing block copolymer as a nanocarrier for porphyrin: energy transfer and singlet oxygen generation in micellar aqueous solution
    Ching Yi Chen
    Department of Materials Science and Engineering, University of Washington, Seattle, Washington 98195 2120, USA
    J Am Chem Soc 129:7220-1. 2007
  73. ncbi Constitutive and agonist-dependent homo-oligomerization of the thyrotropin-releasing hormone receptor. Detection in living cells using bioluminescence resonance energy transfer
    K M Kroeger
    Western Australian Institute for Medical Research, University of Western Australia and Keogh Institute of Medical Research, Sir Charles Gairdner Hospital, Nedlands, Perth, Western Australia 6009, Australia
    J Biol Chem 276:12736-43. 2001
    ..Here, we have employed a newly emerging technology, bioluminescence resonance energy transfer (BRET), used to study protein-protein interactions in living cells, to demonstrate that the thyrotropin-..
  74. pmc Effect of antenna-depletion in Photosystem II on excitation energy transfer in Arabidopsis thaliana
    Bart van Oort
    Wageningen University, The Netherlands
    Biophys J 98:922-31. 2010
    ..photosynthetic antenna complexes of Photosystem II (PSII) both in membrane organization and excitation energy transfer have been investigated...
  75. ncbi Monitoring the conformational fluctuations of DNA hairpins using single-pair fluorescence resonance energy transfer
    J R Grunwell
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    J Am Chem Soc 123:4295-303. 2001
    We present single-pair fluorescence resonance energy transfer (spFRET) observations of individual opening and closing events of surface-immobilized DNA hairpins...
  76. ncbi Application of the fluorescence resonance energy transfer method for studying the dynamics of caspase-3 activation during UV-induced apoptosis in living HeLa cells
    K Q Luo
    Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
    Biochem Biophys Res Commun 283:1054-60. 2001
    ..We have developed a GFP-based FRET (fluorescence resonance energy transfer) probe that is highly sensitive to the activation of caspase-3 in intact living cells...
  77. ncbi Long-range energy transfer in proteins
    Francesco Piazza
    Ecole Polytechnique Federale de Lausanne, Laboratoire de Biophysique Statistique, ITP SB, BSP 720, CH 1015 Lausanne, Switzerland
    Phys Biol 6:046014. 2009
    ..Interestingly, yields are found to be optimum for excitation energies in the range of biologically relevant ones...
  78. ncbi Structural insights into energy regulation of light-harvesting complex CP29 from spinach
    Xiaowei Pan
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
    Nat Struct Mol Biol 18:309-15. 2011
    ..Two special clusters of pigment molecules, namely a615-a611-a612-Lut and Vio(Zea)-a603-a609, have been identified and might function as potential energy-quenching centers and as the exit or entrance in energy-transfer pathways...
  79. ncbi Creatine kinase B-driven energy transfer in the brain is important for habituation and spatial learning behaviour, mossy fibre field size and determination of seizure susceptibility
    Carolina R Jost
    Department of Cell Biology, University Medical Center St Radboud, NCMLS, University of Nijmegen, Geert Grooteplein 28, 6525 GA, The Netherlands
    Eur J Neurosci 15:1692-706. 2002
    ..However, for fuelling of basic physiological activities the role of B-CK can be compensated for by other systems in the versatile and robust metabolic-energy network of the brain...
  80. ncbi Singlet energy dissipation in the photosystem II light-harvesting complex does not involve energy transfer to carotenoids
    Marc G Müller
    Max Planck Institut fur Bioanorganische Chemie, Stiftstrasse 34 36, 45470 Mulheim an der Ruhr, Germany
    Chemphyschem 11:1289-96. 2010
    ..We propose instead that a chlorophyll-chlorophyll charge-transfer state is formed in LHC II oligomers which is an intermediate in the quenching process. The relevance to non-photochemical quenching in vivo is discussed...
  81. pmc Interchain vs. intrachain energy transfer in acceptor-capped conjugated polymers
    D Beljonne
    Chemistry of Novel Materials, University of Mons Hainaut, Place du Parc 20, B 7000 Mons, Belgium
    Proc Natl Acad Sci U S A 99:10982-7. 2002
    ..processes dominate in solution where photoexcitation of the polyindenofluorene units induces a rather slow energy transfer to the perylene end moieties...
  82. ncbi Direct comparison of bioluminescence-based resonance energy transfer methods for monitoring of proteolytic cleavage
    Helen Dacres
    Food Futures Flagship, CSIRO Entomology, Canberra, ACT, Australia
    Anal Biochem 385:194-202. 2009
    Bioluminescence resonance energy transfer (BRET) is a powerful tool for the study of protein-protein interactions and conformational changes within proteins...
  83. pmc How proteins trigger excitation energy transfer in the FMO complex of green sulfur bacteria
    Julia Adolphs
    Institut fur Chemie und Biochemie, Freie Universitat Berlin, Berlin, Germany
    Biophys J 91:2778-97. 2006
    ..this way it becomes possible to understand the molecular mechanism allowing the protein to trigger excitation energy transfer reactions...
  84. ncbi Size- and distance-dependent nanoparticle surface-energy transfer (NSET) method for selective sensing of hepatitis C virus RNA
    Jelani Griffin
    Department of Chemistry, Jackson State University, Jackson, MS, USA
    Chemistry 15:342-51. 2009
    We report size- and distance-dependent surface-energy transfer (SET) properties of gold nanoparticles for recognizing hepatitis C virus (HCV) RNA sequence sensitively and selectively (single-base mutations) in a homogeneous format...
  85. ncbi A novel potassium sensing in aqueous media with a synthetic oligonucleotide derivative. Fluorescence resonance energy transfer associated with Guanine quartet-potassium ion complex formation
    Hiroyuki Ueyama
    Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka 812 8581, Japan
    J Am Chem Soc 124:14286-7. 2002
    ..Fluorescence resonance energy transfer (FRET) to 6-TAMRA from 6-FAM of PSO was observed only in the presence of K+...
  86. pmc Electrostatic model of S4 motion in voltage-gated ion channels
    Harold Lecar
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
    Biophys J 85:2854-64. 2003
    ..The resulting gating-charge distributions are compared to experimental results on wild-type and charge-neutralized mutants of the Shaker K(+) channel...
  87. ncbi Energy transfer from astrocytes to axons: the role of CNS glycogen
    Angus M Brown
    Department of Neurology, University of Washington School of Medicine, Box 3356465, 1959 NE Pacific St, Seattle, WA 98195, USA
    Neurochem Int 45:529-36. 2004
    ....
  88. pmc Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering
    J Zhang
    Department of Pharmacology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 98:14997-5002. 2001
    ..of yellow to cyan emissions in live cells caused by phosphorylation-induced changes in fluorescence resonance energy transfer. The reporter response was accelerated by tethering to PKA holoenzyme and slowed by localization to the ..
  89. pmc Homo- and hetero-oligomeric interactions between G-protein-coupled receptors in living cells monitored by two variants of bioluminescence resonance energy transfer (BRET): hetero-oligomers between receptor subtypes form more efficiently than between less
    Douglas Ramsay
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biochem J 365:429-40. 2002
    ..receptors (GPCRs) were examined in HEK-293 cells using two variants of bioluminescence resonance energy transfer (BRET)...
  90. ncbi Novel, isotype-specific sensors for protein kinase A subunit interaction based on bioluminescence resonance energy transfer (BRET)
    Anke Prinz
    University of Kassel, Department of Biochemistry, Heinrich Plett Str 40, D 34132 Kassel, Germany
    Cell Signal 18:1616-25. 2006
    ..an isoform specific protein kinase A (PKA) subunit interaction assay based on bioluminescence resonance energy transfer (BRET)...
  91. ncbi Deregulated chlorophyll b synthesis reduces the energy transfer rate between photosynthetic pigments and induces photodamage in Arabidopsis thaliana
    Yasuhito Sakuraba
    Institute of Low Temperature Science, Hokkaido University, N19 W8, Kita ku, Sapporo, 060 0819 Japan
    Plant Cell Physiol 51:1055-65. 2010
    ..The energy transfer rates between Chl a molecules in PSII decreased and H(2)O(2) accumulated extensively in the BC plant...
  92. ncbi Monitoring the activation state of the insulin receptor using bioluminescence resonance energy transfer
    N Boute
    , , Paris, France
    Mol Pharmacol 60:640-5. 2001
    We have developed a procedure based on bioluminescence resonance energy transfer (BRET) to monitor the activation state of the insulin receptor in vitro...
  93. pmc Pigment organization and energy transfer dynamics in isolated photosystem I (PSI) complexes from Arabidopsis thaliana depleted of the PSI-G, PSI-K, PSI-L, or PSI-N subunit
    Janne A Ihalainen
    Department of Chemistry, University of Jyvaskyla, Finland
    Biophys J 83:2190-201. 2002
    ....
  94. ncbi Oligomerization of the human serotonin transporter and of the rat GABA transporter 1 visualized by fluorescence resonance energy transfer microscopy in living cells
    J A Schmid
    Institute of Pharmacology, University of Vienna Medical School, Wahringerstrasse 13A, A 1090 Vienna, Austria
    J Biol Chem 276:3805-10. 2001
    ..Oligomers were visualized by fluorescence resonance energy transfer microscopy in living cells using two complementary methods, i.e. ratio imaging and donor photobleaching...
  95. ncbi A human immunodeficiency virus type 1 protease biosensor assay using bioluminescence resonance energy transfer
    Kimberly Hu
    HIV 1 RNA Trafficking Laboratory, Sir Mortimer B Davis Jewish General Hospital, 3999 Cote Ste Catherine Road, Montreal, Que, Canada H3T 1E2
    J Virol Methods 128:93-103. 2005
    ..This assay measures PR activity as a function of the resonance energy transfer (RET) between a donor molecule [humanized sea pansy Renilla reniformis luciferase (hRLuc)] and an energy ..
  96. pmc Third-party bioluminescence resonance energy transfer indicates constitutive association of membrane proteins: application to class a g-protein-coupled receptors and g-proteins
    Sudhakiranmayi Kuravi
    Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, Georgia, USA
    Biophys J 98:2391-9. 2010
    ..Much of the evidence for signaling complexes has come from Förster resonance energy transfer and bioluminescence resonance energy transfer (BRET) studies...
  97. pmc Anomalous surplus energy transfer observed with multiple FRET acceptors
    Srinagesh V Koushik
    National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, Maryland, United States of America
    PLoS ONE 4:e8031. 2009
    Förster resonance energy transfer (FRET) is a mechanism where energy is transferred from an excited donor fluorophore to adjacent chromophores via non-radiative dipole-dipole interactions...
  98. ncbi Spectroscopic characterization of the excitation energy transfer in the fucoxanthin-chlorophyll protein of diatoms
    Emmanouil Papagiannakis
    Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands
    Photosynth Res 86:241-50. 2005
    We characterized the energy transfer pathways in the fucoxanthin-chlorophyll protein (FCP) complex of the diatom Cyclotella meneghiniana by conducting ultrafast transient absorption measurements...
  99. pmc Excitation energy-transfer and the relative orientation of retinal and carotenoid in xanthorhodopsin
    Sergei P Balashov
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    Biophys J 95:2402-14. 2008
    ..From fluorescence studies, we show that energy transfer occurs from the excited singlet S(2) state of salinixanthin to the S(1) state of the retinal...
  100. ncbi Chemiluminescence and chemiluminescence resonance energy transfer (CRET) aptamer sensors using catalytic hemin/G-quadruplexes
    Xiaoqing Liu
    Institute of Chemistry, Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    ACS Nano 5:7648-55. 2011
    ....
  101. pmc Energy migration alters the fluorescence lifetime of Cerulean: implications for fluorescence lifetime imaging Forster resonance energy transfer measurements
    Srinagesh V Koushik
    Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism, Bethesda, Maryland 20852, USA
    J Biomed Opt 13:031204. 2008
    Forster resonance energy transfer (FRET) is a physical phenomenon used to study molecular interactions in living cells...

Research Grants79

  1. Tissue-specific delivery of probes by control of membrane trafficking of endoprot
    Blake Peterson; Fiscal Year: 2010
    ..By flanking members of the peptide library with red and green fluorophores, fluorescence resonance energy transfer (FRET) will allow quantification of in vivo cleavage of members of the peptide library...
  2. Characterizing TLR Function of Human Dendritic Cells in the Elderly
    Alexander Panda; Fiscal Year: 2012
    ..cell biology techniques including pulse-chase experiments, confocal microscopy and fluorescence resonance energy transfer to evaluate age-associated changes in the kinetics of TLR1 surface expression, intracellular distribution and ..
  3. Dynamics of Kinesins, Dyneins, and Myosins
    Paul R Selvin; Fiscal Year: 2010
    ..the study of the conformational changes within the actomyosin complex using lanthanide-based resonance energy transfer (LRET), single molecule FRET. We will utilize an improved "cysteine-light" smooth muscle myosin II...
  4. DNA REPAIR IN A HORMONE RESPONSIVE GENE
    Michael J Smerdon; Fiscal Year: 2013
    ..These studies will involve the use of restriction enzyme accessibility (REA) and Fvrster resonance energy transfer (FRET) to determine the effect of DNA lesions on NCP unwrapping dynamics...
  5. Ionic currents in gastrointestinal smooth muscle
    Hamid I Akbarali; Fiscal Year: 2013
    ..We will also investigate the interaction of src kinase with the calcium channel using fluorescence resonance energy transfer (FRET) and siRNA approaches to delineate the functional effect of src-Ca2+ channel interaction in muscle ..
  6. Post-translational regulation of MLL in leukemogenesis
    ANDREW GEORGE MUNTEAN; Fiscal Year: 2013
    ..MLL-PAFc binding will be monitored by fluorescence resonance energy transfer (FRET) and interaction of candidate chemical compounds will be verified by NMR structural analysis...
  7. Post-translational regulation of MLL in leukemogenesis
    ANDREW GEORGE MUNTEAN; Fiscal Year: 2012
    ..MLL-PAFc binding will be monitored by fluorescence resonance energy transfer (FRET) and interaction of candidate chemical compounds will be verified by NMR structural analysis...
  8. Mechanism and functional role of AKAP79/150 in M current control and excitability
    Mark S Shapiro; Fiscal Year: 2010
    ..Techniques to be used include fluorescence resonance energy transfer (FRET), total internal reflection fluorescence (TIRF), confocal microscopy, patch-clamp electrophysiology, ..
  9. STRUCTURE AND FUNCTION OF PAXILLIN
    Christopher E Turner; Fiscal Year: 2012
    ..microscopy, combined with Fluorescence Recovery after Photobleaching (FRAP) and Fluorescence Resonance Energy Transfer (FRET) analysis to evaluate cell morphology, polarity and migration as well focal adhesion dynamics and ..
  10. Nanotheranostics for Early Colorectal Cancer Detection and Treatment
    Jeffrey S Souris; Fiscal Year: 2013
    ..assessment of polyp M-T staging and therapeutic response, as well as the use of F[unreadable]rster resonance energy transfer (FRET) - between drug and delivery platform - in the characterization of drug releasing dynamics, both in ..
  11. MOLECULAR MODELS OF IMMUNE-MEDIATED VASCULAR INJURY
    Jeffrey R Bender; Fiscal Year: 2012
    ..T cell LFA-1-induced interactions between HuR and hnRNP-C, using pull-down methods, fluorescence resonance energy transfer (FRET) assays, and RNAi-based approaches;and (3) definitively evaluate the role of HuR in coactivator-..
  12. MOLECULAR PROPERTIES OF IONIC PERMEABILITY IN NERVE
    Bertil Hille; Fiscal Year: 2013
    ..The methodology will involve single-cell biophysics, electrophysiology, fluorescence resonance energy transfer, photometry, molecular biology, and confocal microscopy...
  13. FRET-based Biosensors to Monitor Redox in Cell Cycle Regulation
    Paul J A Kenis; Fiscal Year: 2012
    ..These constructs use fluorescence resonance energy transfer (FRET), a distance- and orientation- dependent energy transfer process between donor and acceptor ..
  14. Reciprocal Control of Ca2+ Channels by Anchored Protein Kinase A and Calcineurin
    William A Sather; Fiscal Year: 2010
    ..plan to obtain from single living cells on a microscope stage dynamic measurements of fluorescence resonance energy transfer (FRET) between the various partners in this signaling complex, in an effort to uncover intra- and ..
  15. Excitation-contraction Coupling in Normal and Dystrophic Mammalian Muscle
    Julio L Vergara; Fiscal Year: 2013
    ..be carried out by using electrophysiological and state-of-the-art optical methods, such as F"ster resonance energy transfer (FRET) and total internal reflection fluorescence microscopy (TIRFM), to also assess the nanoscale ..
  16. STRUCTURE AND FUNCTION OF NUCLEIC ACIDS
    Ignacio Tinoco; Fiscal Year: 2013
    ..knowledge, newly discovered single-molecule methods are applied: Laser Tweezers and Fluorescence Resonance Energy Transfer (FRET). RNA molecules are synthesized in the laboratory by RNA polymerase from a DNA template...
  17. Defining the role of Amot lipid binding in cellular proliferation and migration
    ANN CAROL KIMBLE-HILL; Fiscal Year: 2013
    ..contact and insertion of ACCH tyrosines with synthetic membrane environments by F[unreadable]rster resonance energy transfer and small angle scattering...
  18. Flow-induced coronary vasospasm in diabetic patients
    Zsolt Bagi; Fiscal Year: 2013
    ..spatial distribution and interaction of Pecam-1 laser scanning confocal microscopy and fluorescence resonance energy transfer approaches will be used in isolated, pressurized coronary arteries and coronary endothelial cells in culture...
  19. MRP4 extrudes cAMP for localized regulation of calcium channel activity
    OLIVIA RUTH BUONARATI; Fiscal Year: 2013
    ..Levels of cAMP will be monitored by Fluorescence Resonance Energy Transfer upon [unreadable]2AR stimulation with and without MRP4 inhibition or shRNA knockdown (KD)...
  20. Adiponetin and AMPK Biosensor
    John Y J Shyy; Fiscal Year: 2010
    ..In addition, we developed a prototype of a fluorescence resonance energy transfer (FRET)-based AMPK activity reporter (AMPKAR) to measure AMPK activity...
  21. LIPID MODULATION OF RHODOPSIN SIGNALING IN MEMBRANES
    Michael F Brown; Fiscal Year: 2010
    ..In addition, Fourier transform infrared (FTIR), fluorescence resonance energy transfer (FRET), and plasmon waveguide resonance (PWR) spectroscopy will elucidate the retinal environment, protein ..
  22. Advanced Immunocytochemistry, In Situ Hybridization and Live Cell Imaging Course
    TERRI I GRODZICKER; Fiscal Year: 2013
    ..in living cells using standard fluorescence, fluorescence recovery after photobleaching, and resonance energy transfer. All experiments are performed using a range of state-of-the-art microscope methods (epifluorescence, ..
  23. EBP50 Regulation of PTH Receptor in Bone
    Peter A Friedman; Fiscal Year: 2013
    ..the effect of amino acid mutation on binding affinity, and biophysical measurements of fluorescence resonance energy transfer microscopy to acquire dynamic interactions in living cells and in real time, and surface plasmon resonance to ..
  24. Regulation and Catalysis of Human Insulin Degrading Enzyme
    Wei Jen Tang; Fiscal Year: 2013
    ..and functions of IDE in three specific aims: in Aim 1, we will use single molecule Forster resonance energy transfer analyses to address the conformational switches and dynamics that occur during catalysis of IDE and to ..
  25. FKBP Interaction with RYR Calcium Channels in Heart
    Donald M Bers; Fiscal Year: 2013
    ..in cardiac myocytes and isolated SR and RyRs (using fluorescent- tagged FKBP and fluorescence resonance energy transfer (FRET). Four Specific Aims will: 1) identify key FKBP12 &12...
  26. NEUTROPHIL FLAVOCYTOCHROME B STRUCTURE AND FUNCTION
    ALGIRDAS JOSEPH JESAITIS; Fiscal Year: 2010
    ..modify these antibodies with fluorescent probes, to triangulate heme sites using fluorescence resonance energy transfer, measure the distance between antibody sites, and determine how these distances change upon activation of the ..
  27. Single Molecule Dynamics of mRNA Translation
    Yale E Goldman; Fiscal Year: 2013
    ..Total Internal Reflection Fluorescence Microscopy (TIRFM) with single molecule Fluorescence Resonance Energy Transfer (smFRET)...
  28. Zeiss 2-photon (2P) LSM780 laser scanning confocal microscope
    Moshe Levi; Fiscal Year: 2013
    ..FLIM is also a very sensitive technique to perform Foster (or Fluorescence) Resonance Energy Transfer (FRET), a powerful technique to monitor protein-protein interactions of biological interest that occur within ..
  29. Structure and dynamics of the Tetrahymena telomerase ribonucleoprotein
    Michael D Stone; Fiscal Year: 2013
    ..In aim 1, we will use chemical RNA probing and single molecule Forster resonance energy transfer (smFRET) to characterize the telomerase RNA solution structure and dynamics, respectively...
  30. Structural and Mechanistic Characterization of the Histone Chaperone FACT
    Duane David Winkler; Fiscal Year: 2012
    ..Second, solution-based binding, competition, and fluorescence resonance energy transfer (FRET) assays will provide important mechanistic information on FACT mediated nucleosome assembly/disassembly...
  31. smFRET Investigation of Gating in a Neurotransmitter Transporter Homolog
    RACHEL ANN KOLSTER; Fiscal Year: 2013
    ..To address this shortcoming, we have developed single-molecule Forster resonance energy transfer (smFRET) methods that enable us to directly measure conformational dynamics in LeuT in real time...
  32. Design and Implementation of Genetically Encoded Myosin Based Force Sensors
    DANIEL PETER KIEHART; Fiscal Year: 2013
    ..Aim 1: We will construct genetically encoded Tension Sensing Modules (TSMs) based on Fluorescence Resonance Energy Transfer (FRET) pairs separated by protein springs...
  33. Structural Dynamics of Cardiac Muscle Contraction
    Brett A Colson; Fiscal Year: 2012
    ..3) Use time-resolved phosphorescence anisotropy (TPA) and fluorescence resonance energy transfer (FRET), with probes on actin or myosin in solution, to determine the effects of purified recombinant cMyBP-C ..
  34. EGF Receptor Signaling in Time and Space in Tumor Cells
    Alexander D Sorkin; Fiscal Year: 2012
    ..a combination of RNA interference, live-cell fluorescence imaging, multi-chromophore fluorescence resonance energy transfer microscopy and biochemical techniques, the mechanisms of the formation of the multi-protein complexes during ..
  35. Role of nAChR stoichiometry in nicotine-induced upregulation
    CHRISTOPHER I RICHARDS; Fiscal Year: 2012
    ..neurons will be investigated using total internal reflection microscopy (TIRFM) and fluorescence resonance energy transfer (FRET) at both the single vesicle and single receptor level...
  36. Nano-sized Cell Guidance System for Ischemic Tissue Repair
    Hyunjoon Kong; Fiscal Year: 2010
    ..Specifically, we will use a fluorescence resonance energy transfer (FRET) technique we previously developed to quantify the number of poly(glycerol) bound to EPCs...
  37. Voltage-Gating in Bacterial Ion Channels
    Ana M Correa; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE: Using state-of-the-art techniques in electrophysiology and spectroscopy (lanthanide energy transfer), combined with molecular biology, we propose here to determine in vivo the movement of crucial functional ..
  38. STRUCTURE-FUNCTION RELATIONSHIPS OF IMMUNORECEPTORS
    Barbara A Baird; Fiscal Year: 2013
    ..between Fc5RI and order- vs disorder- preferring lipids will be measured with fluorescence resonance energy transfer. Cholesterol distribution across the outer and inner leaflets of the plasma membrane will be assessed, and ..
  39. MOLECULAR PROPERTIES OF IONIC PERMEABILITY IN NERVE
    Bertil Hille; Fiscal Year: 2012
    ..The methods will include patch-clamp electrophysiology, fluorescence resonance energy transfer, dynamic targeting of enzymes to cellular membranes, confocal microscopy, and chemical analysis...
  40. HTS ASSAYS FOR INHIBITORS OF PROTEIN INTERACTIONS INCLUDING MLCK1-FKBP8
    Jerrold R Turner; Fiscal Year: 2012
    ..The goals of our study are i) to develop time-resolved, F[unreadable]rster Resonance Energy Transfer (TR-FRET) assays for screening protein-protein interaction (PPI) inhibitors in vitro and inside living cells;..
  41. QD-BRET nanosensors for protease detection and imaging
    Jianghong Rao; Fiscal Year: 2012
    ..This new platform is based on quantum dots (QDs) and bioluminescence resonance energy transfer (BRET)...
  42. HIV integrase/DNA complexes and concerted integration
    Duane P Grandgenett; Fiscal Year: 2010
    ..In- gel fluorescence resonance energy transfer-derived distance measurements and atomic force microscopy will determine the topology of the two viral DNA ..
  43. Homogenous HTS Assays to Screen for Inhibitors of Keap1-Nrf2 Interaction
    Longqin Hu; Fiscal Year: 2010
    ..in this new R01 application using fluorescence polarization (FP) and time-resolved fluorescence resonance energy transfer (TR-FRET)...
  44. Real-time microviscosity measurement tools for the cell
    Mark A Haidekker; Fiscal Year: 2010
    ..in which a molecular rotor and a fluorescent reference unit form a covalently linked dye pair for resonance energy transfer (RET)...
  45. Novel Fluorescent Nucleotide Analogues to Probe Nucleic Acid Metabolism
    BYRON WILLIS PURSE; Fiscal Year: 2010
    ..of the absorption and emission window and the brightening of the dyes, new FRET (Fluorescence Resonance Energy Transfer) applications and direct in vivo fluorescence imaging of DNA synthesis are likely to be possible...
  46. FRET Imaging of Molecular Hierarchy at Subcellular Levels in Mechanotransduction
    Yingxiao Wang; Fiscal Year: 2013
    ..Fluorescence resonance energy transfer (FRET) technology and genetically encoded biosensors have provided powerful tools for visualizing active ..
  47. Elucidating Protein Folding Mechanisms
    Jay R Winkler; Fiscal Year: 2010
    ..Fluorescence energy transfer methods will be used in Aim 1 to define distributions of distances between pairs of residues in helical-..
  48. Spliceosome Mechanism Dissected at the Single Molecule Level
    Nils G Walter; Fiscal Year: 2013
    ..To address this challenge, we recently have developed single molecule fluorescence resonance energy transfer (smFRET) assays that have begun to dissect pre-mRNA conformational changes during splicing...
  49. Molecular Mechanisms of Cytoskeletal Regulators
    Kevin C Slep; Fiscal Year: 2013
    ..This examination will use in vitro tubulin and microtubule binding assays as well as a F"rster resonance energy transfer assay to generate a model of the TOG-tubulin complex...
  50. Mice with Functional Fluorescent Nicotinic Receptor Subunits
    Henry A Lester; Fiscal Year: 2010
    ..allele will be generated, so that strains can be crossed to study receptor assembly with Foerster resonance energy transfer. Fluorescent proteins (FPs) have been successfully integrated into the M3-M4 intracellular loop of most of ..
  51. NSAID-Phosphatidylcholine Association: Insight in GI Ulcer Pathogenesis/Therapy
    Lenard M Lichtenberger; Fiscal Year: 2010
    ..using: dynamic light scattering for size determination, and fluorescent probes such as, fluorescent resonance energy transfer (FRET) and dye exclusion analysis to assess the effects of NSAID bile acid interactions on membrane fluidity, ..
  52. Antigen Binding, Crosslinking and Transmembrane Signaling
    David A Holowka; Fiscal Year: 2013
    ..via recently identified Orai1/CRACM1 calcium channels will be characterized using fluorescence resonance energy transfer imaging for measurements of molecular proximity between this channel and the calcium sensor, STIM1...
  53. Signaling complex remodeling on the surfaces of live macrophages
    SHALINI THERESE LOW-NAM; Fiscal Year: 2013
    ..dynamics will be imaged using fluorescent chimeric receptor and quantified by Fluorescence Resonance Energy Transfer (FRET)...
  54. Site-Directed Oxidative Modification of Muscle Protein Structural Dynamics
    David D Thomas; Fiscal Year: 2013
    ..A complementary array of spectroscopic techniques will be used - fluorescence resonance energy transfer (FRET), transient phosphorescence anisotropy (TPA), electron paramagnetic resonance (EPR), and nuclear ..
  55. Spinning Disk Confocoal Microscope
    Judy Drazba; Fiscal Year: 2010
    ..It will also provide the ability to do fluorescence resonance energy transfer (FRET) measurements, fluorescence recovery after photobleaching (FRAP) measurements and photoactivation, ..
  56. HTS to identify chemical probes of the apelin receptor signaling pathway
    Layton Harris Smith; Fiscal Year: 2010
    ..The second assay uses the commercially available Lance homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) assay to measure cAMP accumulation...
  57. Development of lisinopril for post-exposure mitigation of late effects from a rad
    MEETHA M MEDHORA; Fiscal Year: 2013
    ..We will also test lisinopril for mitigation after exposure to single low- and high-linear energy transfer irradiation (X-rays, neutron-gamma rays), and to radiation in combination with full thickness skin wounds ..
  58. New probes for matrix metalloproteinase 13
    Gregg B Fields; Fiscal Year: 2013
    ..Our laboratory has developed triple-helical peptide (THP) fluorescence resonance energy transfer (FRET) substrates for high throughput screening (HTS) of collagenolytic MMPs and identification of putative ..
  59. Protein Kinase Regulatory Subunit I Directed Mutagenesis
    Susan S Taylor; Fiscal Year: 2013
    ..angle Xray scattering, small angle neutron scattering, stopped-flow fluorescence, fluorescence resonance energy transfer, hydrogen/deuterium exchange coupled with mass spectrometry, and NMR spectroscopy in parallel with Xray ..
  60. ION GRADIENTS AND ENERGY COUPLING IN BACTERIA
    Peter C Maloney; Fiscal Year: 2012
    ..place pairs of fluorescent probes at strategic positions on OxlT and use single-pair fluorescence resonance energy transfer (spFRET) to measure separation distances changes at the single-molecule level...
  61. Systems Biology Investigation of Muscle Exercise Metabolism in Diabetes
    Nicola Lai; Fiscal Year: 2013
    ..The research training emphasizes quantitative understanding of the regulation of cellular energy transfer and metabolism in myocyte and whole skeletal muscle in response to energy demand in control and diabetic rats...
  62. PEPTIDE-CELL INTERACTIONS IN SACCHAROMYCES CEREVISIAE
    Fred R Naider; Fiscal Year: 2011
    ..mutagenesis, unnatural amino acid replacement, native chemical ligation, bioluminescent resonance energy transfer, and multidimensional nuclear magnetic resonance spectroscopy...
  63. Bacterial Response to Singlet Oxygen
    Timothy Donohue; Fiscal Year: 2009
    ..converted to different chemical classes of toxic reactive oxygen species by either one electron transfer or energy transfer reactions...
  64. Regulation of Epithelial Sodium Channel by CFTR chloride channel
    Bakhrom Berdiev; Fiscal Year: 2009
    ..We will determine the CFTR-ENaC interaction using Fluorescence Resonance Energy Transfer (FRET) microscopy and co-immunoprecipitation (co-IP)...
  65. TUMOR-TARGETING WITH NEW BORONATED PORPHYRINS
    MARIA DA GRACA HENRIQUES VICENTE; Fiscal Year: 2010
    ..which occurs when boron-10 nuclei localized within tumors capture low-energy neutrons to produce high linear energy transfer particles whose cytotoxic effects are confined to the cells in which the drug is retained...
  66. ARCHITECTURE OF ERYTHROCYTE SKELETON--BILAYER INTERFACE
    STEPHEN SHOHET; Fiscal Year: 2000
    ..1 binding region to the bilayer. These measurements will be determined by fluorescence energy transfer (FET) and single-photon radioluminescence...
  67. Protection against radiation-induced damage to intestinal nutrient transport
    Roger Howell; Fiscal Year: 2009
    ..may be delivered acutely or chronically, by external or internal sources, and by low- and high-linear energy transfer (LET) radiations...
  68. DNA Sequencing at a Stretch
    Di Gao; Fiscal Year: 2009
    ..Through a fluorescence resonance energy transfer (FRET) mechanism, detachment of the DNA strand can be directly detected from the fluorescence signals, which ..
  69. PHOSPHORIMAGER
    Allan Brasier; Fiscal Year: 1993
    ..used in molecular biological and biochemical experimentation and relies on, and is limited by, the physics of energy transfer between incident radioactive particles and silver halide crystals embedded in a film emulsion...
  70. INTERSTITIAL NEUTRON THERAPY FOR GLIOBLASTOMA
    ALFRED YOUNG; Fiscal Year: 1991
    ..Neutron radiation, which irradiates with high linear energy transfer (LET) radiation, has theoretical advantages over conventional low LET photon (x- ray) therapy, including: 1) ..
  71. Lipid modulation of beta-cell calcium channels
    Lina Moitoso de Vargas; Fiscal Year: 2006
    ..Our preliminary data from the fluorescence resonance energy transfer analyses indicate that the L-type channel predominantly expressed in beta-cells, the neuroendocrine alpha1D ..
  72. Phase I / II Trial for Neutron Capture Therapy
    Matthew Palmer; Fiscal Year: 2002
    Neutron capture therapy (NCT) is a unique form of radiation due to the combination of high linear energy transfer (LET) properties and biological targeting...
  73. Real-time Single-Molecule Analysis for Transient Molec. Complex Characterization
    Taiho Kim; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Single-molecule fluorescence resonance energy transfer (smFRET) has rapidly developed to answer fundamental questions about biological mechanisms, providing detailed views of molecular machines at ..
  74. Discovery of novel fluorescent reporter genes
    Ming Zhao; Fiscal Year: 2004
    ..for minimizing background emission and scattering in vivo as well as for FRET (fluorescence resonance energy transfer) analysis...
  75. Real-time measurement of ethanol's effect on cyclic AMP metabolism in live cells
    Masami Yoshimura; Fiscal Year: 2009
    ..chain cAMP sensor molecules have been developed, which can monitor cAMP levels by fluorescence resonance energy transfer (FRET)...
  76. Enhanced Liquid Core Waveguide Biosensor for PBDE Detection
    HEE JOO contact KIM; Fiscal Year: 2010
    ..specific phage-borne peptide as the key technology, Synthia-LLC will develop a homogeneous fluorescent energy transfer PHAIA in a liquid core waveguide (FRET PHAIA-LCW) by setting three aims to achieve the goal...
  77. High Sensitivity Multi-Well Plate Reader Based on an Array of Photon Counting Det
    William Lawrence; Fiscal Year: 2007
    ..include absorbance, fluorescence intensity and polarization, chemiluminescence, fluorescence resonance energy transfer (FRET) and time resolved fluorescence...
  78. A FRET-based assay to measure dynamic changes in RyR1 structure
    JAMES FESSENDEN; Fiscal Year: 2009
    ..an innovative method to map the overall tertiary and quaternary structure of RyR1 using Forester resonance energy transfer (FRET) techniques...
  79. Detection of Bacteria using Nanoparticle-Polymer Sensors
    VINCENT ROTELLO; Fiscal Year: 2009
    ..In these conjugates fluorescence emission from the polymer is quenched via energy transfer to the nanoparticle...