Summary: The formation of cartilage. This process is directed by CHONDROCYTES which continually divide and lay down matrix during development. It is sometimes a precursor to OSTEOGENESIS.

Top Publications

  1. ncbi Wnt/beta-catenin signaling in mesenchymal progenitors controls osteoblast and chondrocyte differentiation during vertebrate skeletogenesis
    Timothy F Day
    Geneti Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 8:739-50. 2005
  2. ncbi Reaching a genetic and molecular understanding of skeletal development
    Gerard Karsenty
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Dev Cell 2:389-406. 2002
  3. ncbi The expression and function of microRNAs in chondrogenesis and osteoarthritis
    Tracey E Swingler
    University of East Anglia, Norwich, UK
    Arthritis Rheum 64:1909-19. 2012
  4. pmc Genetic analysis of the roles of BMP2, BMP4, and BMP7 in limb patterning and skeletogenesis
    Amitabha Bandyopadhyay
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Genet 2:e216. 2006
  5. ncbi Mesenchymal stem cells: clinical applications and biological characterization
    Frank P Barry
    Osiris Therapeutics Inc, 2001 Aliceanna Street, Baltimore, MD 21231, USA
    Int J Biochem Cell Biol 36:568-84. 2004
  6. ncbi Chondrogenesis of human mesenchymal stem cells mediated by the combination of SOX trio SOX5, 6, and 9 genes complexed with PEI-modified PLGA nanoparticles
    Ji Sun Park
    Department of Biomedical Science, College of Life Science, CHA University, 606 16 Yeoksam 1 dong, Kangnam Gu, Seoul 135 081, Republic of Korea
    Biomaterials 32:3679-88. 2011
  7. ncbi MicroRNAs regulate osteogenesis and chondrogenesis
    Shiwu Dong
    Laboratory of Biomechanics, Department of Anatomy, The Third Military Medical University, Chongqing, China
    Biochem Biophys Res Commun 418:587-91. 2012
  8. ncbi Mid-term results of Autologous Matrix-Induced Chondrogenesis for treatment of focal cartilage defects in the knee
    J Gille
    Department of Orthopaedics and Trauma, University Hospital Schleswig Holstein, Campus Lubeck, Ratzeburger Allee 160, Lubeck, Germany
    Knee Surg Sports Traumatol Arthrosc 18:1456-64. 2010
  9. ncbi Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle
    Hideya Yoshimura
    Section of Orthopaedic Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan
    Cell Tissue Res 327:449-62. 2007
  10. ncbi Hedgehog signaling is required for cranial neural crest morphogenesis and chondrogenesis at the midline in the zebrafish skull
    Naoyuki Wada
    Department of Developmental and Cell Biology, University of California, Irvine, 5210 McGaugh Hall, Irvine, CA 92697 2300, USA
    Development 132:3977-88. 2005

Detail Information

Publications309 found, 100 shown here

  1. ncbi Wnt/beta-catenin signaling in mesenchymal progenitors controls osteoblast and chondrocyte differentiation during vertebrate skeletogenesis
    Timothy F Day
    Geneti Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 8:739-50. 2005
    ..Controlling Wnt/beta-catenin signaling is a common molecular mechanism underlying chondrocyte and osteoblast differentiation and specification of intramembranous and endochondral ossification...
  2. ncbi Reaching a genetic and molecular understanding of skeletal development
    Gerard Karsenty
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Dev Cell 2:389-406. 2002
    ..made considerable progress in our genetic and molecular understanding of all aspects of skeletal development, chondrogenesis, joint formation, and osteogenesis...
  3. ncbi The expression and function of microRNAs in chondrogenesis and osteoarthritis
    Tracey E Swingler
    University of East Anglia, Norwich, UK
    Arthritis Rheum 64:1909-19. 2012
    To use an in vitro model of chondrogenesis to identify microRNAs (miRNAs) with a functional role in cartilage homeostasis.
  4. pmc Genetic analysis of the roles of BMP2, BMP4, and BMP7 in limb patterning and skeletogenesis
    Amitabha Bandyopadhyay
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Genet 2:e216. 2006
    ..We do find that a threshold level of BMP signaling is required for the onset of chondrogenesis, and hence some chondrogenic condensations fail to form in limbs deficient in both BMP2 and BMP4...
  5. ncbi Mesenchymal stem cells: clinical applications and biological characterization
    Frank P Barry
    Osiris Therapeutics Inc, 2001 Aliceanna Street, Baltimore, MD 21231, USA
    Int J Biochem Cell Biol 36:568-84. 2004
    ..These include the host immune response to implanted cells, the homing mechanisms that guide delivered cells to a site of injury and the differentiation in vivo of implanted cells under the influence of local signals...
  6. ncbi Chondrogenesis of human mesenchymal stem cells mediated by the combination of SOX trio SOX5, 6, and 9 genes complexed with PEI-modified PLGA nanoparticles
    Ji Sun Park
    Department of Biomedical Science, College of Life Science, CHA University, 606 16 Yeoksam 1 dong, Kangnam Gu, Seoul 135 081, Republic of Korea
    Biomaterials 32:3679-88. 2011
    ..was evaluated to investigate the potential for transfection efficiency to increase the potential ability of chondrogenesis when the trio genes (SOX5, 6, and 9) polyplexed with PEI were delivered into hMSCs...
  7. ncbi MicroRNAs regulate osteogenesis and chondrogenesis
    Shiwu Dong
    Laboratory of Biomechanics, Department of Anatomy, The Third Military Medical University, Chongqing, China
    Biochem Biophys Res Commun 418:587-91. 2012
    ..Here, we review the recent research progress on the regulation of miRNAs in bone biology, with a particular focus on the miRNA-mediated control mechanisms of bone and cartilage formation...
  8. ncbi Mid-term results of Autologous Matrix-Induced Chondrogenesis for treatment of focal cartilage defects in the knee
    J Gille
    Department of Orthopaedics and Trauma, University Hospital Schleswig Holstein, Campus Lubeck, Ratzeburger Allee 160, Lubeck, Germany
    Knee Surg Sports Traumatol Arthrosc 18:1456-64. 2010
    Articular cartilage defects heal poorly. Autologous Matrix-Induced Chondrogenesis (AMIC) is an innovative treatment for localized full-thickness cartilage defects combining the well-known microfracturing with collagen scaffold and fibrin ..
  9. ncbi Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle
    Hideya Yoshimura
    Section of Orthopaedic Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan
    Cell Tissue Res 327:449-62. 2007
    ..An in vitro chondrogenesis assay demonstrated that the pellets derived from synovium were heavier, because of their greater production of ..
  10. ncbi Hedgehog signaling is required for cranial neural crest morphogenesis and chondrogenesis at the midline in the zebrafish skull
    Naoyuki Wada
    Department of Developmental and Cell Biology, University of California, Irvine, 5210 McGaugh Hall, Irvine, CA 92697 2300, USA
    Development 132:3977-88. 2005
    ..although requirements in morphogenesis occur during neural crest migration beneath the brain, requirements in chondrogenesis occur later, as cells form separate trabecular and ethmoid condensations...
  11. pmc Connective tissue growth factor coordinates chondrogenesis and angiogenesis during skeletal development
    Sanja Ivkovic
    Department of Orthopaedic Surgery, David Geffen School of Medicine at UCLA, University of California, Los Angeles, CA 90095, USA
    Development 130:2779-91. 2003
    ..These results demonstrate that CTGF is important for cell proliferation and matrix remodeling during chondrogenesis, and is a key regulator coupling extracellular matrix remodeling to angiogenesis at the growth plate.
  12. pmc Chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells in self-gelling alginate discs reveals novel chondrogenic signature gene clusters
    Sarah Roxana Herlofsen
    Norwegian Center for Stem Cell Research, Oslo University Hospital Rikshospitalet, University of Oslo, Oslo, Norway
    Tissue Eng Part A 17:1003-13. 2011 perform a detailed kinetic study of the expression of a range of genes and proteins known to be involved in chondrogenesis, using real-time polymerase chain reaction, fluorescence immunohistochemistry, and glycosaminoglycan ..
  13. ncbi FGF signaling pathways in endochondral and intramembranous bone development and human genetic disease
    David M Ornitz
    Department of Molecular Biology and Pharmacology, Washington University Medical School, St Louis, Missouri 63110, USA
    Genes Dev 16:1446-65. 2002
  14. ncbi The treatment of chondral and osteochondral defects of the knee with autologous matrix-induced chondrogenesis (AMIC): method description and recent developments
    Jan Philipp Benthien
    Department of Orthopaedic Surgery, University of Basel, Basel, Switzerland
    Knee Surg Sports Traumatol Arthrosc 19:1316-9. 2011
    ..This technical note describes the method of autologous matrix-induced chondrogenesis (AMIC), a one-step procedure combining subchondral microfracture with the fixation of a collagen I/III ..
  15. ncbi Fibroblast growth factor receptors in in vitro and in vivo chondrogenesis: relating tissue engineering using adult mesenchymal stem cells to embryonic development
    Catharine A Hellingman
    Department of Otorhinolaryngology, University Medical Center Rotterdam, Rotterdam, The Netherlands
    Tissue Eng Part A 16:545-56. 2010
    ..this study was to investigate and compare FGFR expression in in vivo embryonic limb development and in vitro chondrogenesis of MSCs...
  16. pmc Isolation of adipose-derived stem cells and their induction to a chondrogenic phenotype
    Bradley T Estes
    Departments of Surgery and Biomedical Engineering, Duke University Medical Center, Durham, North Carolina, USA
    Nat Protoc 5:1294-311. 2010
    ..These methods are similar to those published for chondrogenesis of bone marrow-derived mesenchymal stem cells but distinct because of the unique nature of ASCs...
  17. ncbi FGF-2 enhances the mitotic and chondrogenic potentials of human adult bone marrow-derived mesenchymal stem cells
    Luis A Solchaga
    Department of Orthopaedics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
    J Cell Physiol 203:398-409. 2005
    ..With the generation of gene expression profiles of FGF-treated and control cells we have taken the first steps in the elucidation of the molecular mechanism(s) behind these phenomena...
  18. ncbi Regulation of endoplasmic reticulum stress response by a BBF2H7-mediated Sec23a pathway is essential for chondrogenesis
    Atsushi Saito
    Division of Molecular and Cellular Biology, Department of Anatomy, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889 1692, Japan
    Nat Cell Biol 11:1197-204. 2009
    ..restored, indicating that by activating protein secretion the BBF2H7-Sec23a pathway has a crucial role in chondrogenesis. Our findings provide a new link by which ER stress is converted to signalling for the activation of ER-to-..
  19. pmc Bmpr1a and Bmpr1b have overlapping functions and are essential for chondrogenesis in vivo
    Byeong S Yoon
    Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 102:5062-7. 2005
    ..However, the in vivo role of BMP signaling during chondrogenesis has been unclear. We report here that BMP signaling is essential for multiple aspects of early chondrogenesis...
  20. pmc miR-199a, a bone morphogenic protein 2-responsive MicroRNA, regulates chondrogenesis via direct targeting to Smad1
    Edward A Lin
    Department of Orthopaedic Surgery, New York University School of Medicine, New York, New York 10003, USA
    J Biol Chem 284:11326-35. 2009
    ..reduced at 5 h, quickly increased at 24 h and remained higher thereafter in the course of BMP2-triggered chondrogenesis of a micromass culture of pluripotent C3H10T1/2 stem cells...
  21. pmc miR-449a regulates the chondrogenesis of human mesenchymal stem cells through direct targeting of lymphoid enhancer-binding factor-1
    Seungil Paik
    Department of Orthopaedic Surgery, Yonsei University College of Medicine, Seoul, South Korea
    Stem Cells Dev 21:3298-308. 2012
    ..In this study, we determined that the lymphoid enhancer-binding factor-1 (LEF-1) was expressed during the chondrogenesis of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) and sought to identify a novel microRNA ..
  22. ncbi Regulation of chondrocyte differentiation by the actin cytoskeleton and adhesive interactions
    Anita Woods
    CIHR Group in Skeletal Development and Remodeling, Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada
    J Cell Physiol 213:1-8. 2007
    ..Cell-matrix interactions are also important for chondrogenesis, for example through the activity of CD44, a receptor for Hyaluronan and collagens...
  23. pmc Major biological obstacles for persistent cell-based regeneration of articular cartilage
    Andre F Steinert
    Orthopaedic Center for Musculoskeletal Research, König Ludwig Haus, Julius Maximilians University, Wurzburg, Germany
    Arthritis Res Ther 9:213. 2007
    ..The current conceptual basis of the major biological obstacles for persistent cell-based regeneration of articular cartilage is discussed, as well as future trends to overcome these limitations...
  24. ncbi Combination of transforming growth factor-beta2 and bone morphogenetic protein 7 enhances chondrogenesis from adipose tissue-derived mesenchymal stem cells
    Hye Joung Kim
    Department of Orthopedics, Dongguk University International Hospital, Goyang, Korea
    Tissue Eng Part A 15:1543-51. 2009
    In this study, the authors examined combinations of growth factors that induce effective chondrogenesis from adipose tissue-derived mesenchymal stem cells (MSCs)...
  25. ncbi Trps1 plays a pivotal role downstream of Gdf5 signaling in promoting chondrogenesis and apoptosis of ATDC5 cells
    Shunji Itoh
    First Department of Pathology, Wakayama Medical University, Wakayama, Japan
    Genes Cells 13:355-63. 2008
    ..In conclusion, Trps1 acts downstream of the Gdf5 signaling pathway and promotes the differentiation and apoptosis of ATDC5 cells...
  26. pmc Macromer density influences mesenchymal stem cell chondrogenesis and maturation in photocrosslinked hyaluronic acid hydrogels
    I E Erickson
    McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA 19104, USA
    Osteoarthritis Cartilage 17:1639-48. 2009
    ..In this study, we investigated the ability of bone marrow derived mesenchymal stem cells (MSCs) to undergo chondrogenesis in crosslinked methacrylated hyaluronic acid hydrogels (MeHA) of different macromer concentrations (1, 2, and ..
  27. ncbi MicroRNA-337 is associated with chondrogenesis through regulating TGFBR2 expression
    N Zhong
    Department of Genetics and Molecular Biology, Xi an Jiaotong University College of Medicine, Xi an, Shaanxi 710061, PR China
    Osteoarthritis Cartilage 20:593-602. 2012
    ..This study aimed to examine six miRNAs expression during the cartilage development and identify the key miRNA which is associated with chondrogenesis.
  28. pmc Hypertrophy in mesenchymal stem cell chondrogenesis: effect of TGF-beta isoforms and chondrogenic conditioning
    Michael B Mueller
    Department for Trauma Surgery, Regensburg University Medical Center, Regensburg, Germany
    Cells Tissues Organs 192:158-66. 2010
    Induction of chondrogenesis in mesenchymal stem cells (MSCs) with TGF-beta leads to a hypertrophic phenotype...
  29. pmc Proliferation as a requirement for in vitro chondrogenesis of human mesenchymal stem cells
    Verena Dexheimer
    Research Centre for Experimental Orthopaedics, Orthopaedic University Hospital Heidelberg, Heidelberg, Germany
    Stem Cells Dev 21:2160-9. 2012
    ..are imitated during in vitro differentiation of mesenchymal stem cells (MSCs) and are crucial for a proper chondrogenesis. Human MSCs were expanded in distinct media and subjected to pellet culture in chondrogenic medium...
  30. ncbi Chondrocytes or adult stem cells for cartilage repair: the indisputable role of growth factors
    Anne Marie Freyria
    Cartilage Biology and Engineering Group, IBCP, Universite Lyon 1, Univ Lyon, CNRS FRE 3310, IFR128, France
    Injury 43:259-65. 2012
    ..cell proliferation, extracellular matrix synthesis, maintenance of the phenotype to induction of MSCs towards chondrogenesis. Members of the TGF-β superfamily, are especially important in fulfilling these roles...
  31. pmc Unraveling the transcriptional regulatory machinery in chondrogenesis
    Haruhiko Akiyama
    Department of Orthopaedics, Kyoto University, 54 Kawahara cho, Shogoin, Sakyo, Kyoto 606 8507, Japan
    J Bone Miner Metab 29:390-5. 2011
    ..We review here the discovery of this master chondrogenic trio and its roles in chondrogenesis in vivo and at the molecular level, and we discuss how these pioneering studies open the way for many ..
  32. ncbi Combined effects of insulin-like growth factor-1 and transforming growth factor-beta1 on periosteal mesenchymal cells during chondrogenesis in vitro
    T Fukumoto
    Cartilage and Connective Tissue Research Laboratory, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Osteoarthritis Cartilage 11:55-64. 2003
    ..During this process, the role of growth factors that stimulate the periosteal mesenchymal cells toward chondrogenesis to regenerate articular cartilage and maintain its phenotype is not yet fully understood...
  33. ncbi Autologous matrix-induced chondrogenesis combined with platelet-rich plasma gel: technical description and a five pilot patients report
    A A M Dhollander
    Department of Orthopaedic Surgery and Traumatology, Ghent University Hospital, De Pintelaan 185 2P5, 9000, Ghent, Belgium
    Knee Surg Sports Traumatol Arthrosc 19:536-42. 2011
    ..designed to describe the technical details and to present the preliminary outcome of autologous matrix-induced chondrogenesis (AMIC) combined with platelet-rich plasma gel, the so called AMIC plus technique, for the treatment of ..
  34. ncbi Trophic effects of mesenchymal stem cells increase chondrocyte proliferation and matrix formation
    Ling Wu
    Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands
    Tissue Eng Part A 17:1425-36. 2011
  35. ncbi Chondrogenesis, osteogenesis and adipogenesis of canine mesenchymal stem cells: a biochemical, morphological and ultrastructural study
    C Csaki
    Institute of Anatomy, Musculoskeletal Research Group, Ludwig Maximilian University Munich, Pettenkoferstrasse 11, 80336, Munich, Germany
    Histochem Cell Biol 128:507-20. 2007
    ..These results demonstrate, at the biochemical, morphological and ultrastructural levels, the multipotency of cMSCs and thus highlight their potential therapeutic value for cell-based tissue engineering...
  36. ncbi PTHrP promotes chondrogenesis and suppresses hypertrophy from both bone marrow-derived and adipose tissue-derived MSCs
    Young Ju Kim
    Department of Orthopaedics, Dongguk University International Hospital, 814 Siksa dong, Goyang 411 773, Republic of Korea
    Biochem Biophys Res Commun 373:104-8. 2008
    During chondrogenesis from mesenchymal stem cells (MSCs), inadequate differentiation and hypertrophic differentiation are two important limitations...
  37. pmc The influence of ferucarbotran on the chondrogenesis of human mesenchymal stem cells
    Tobias D Henning
    Department of Radiology, UCSF Medical Center, University of California, San Francisco, CA 94143 0628, USA
    Contrast Media Mol Imaging 4:165-73. 2009
    ..After labeling by simple incubation, chondrogenesis was found to be dose-dependent...
  38. ncbi The use of mesenchymal stem cells for chondrogenesis
    Karoliina Pelttari
    Division of Experimental Orthopaedics, Orthopaedic University Hospital Heidelberg, Schlierbacher Landstrasse 2002, Heidelberg, Germany
    Injury 39:S58-65. 2008
    ..However, in vitro chondrogenesis of MSC additionally promotes induction of fibrocartilage-like features such as expression of collagen type I, ..
  39. ncbi Hypoxia promotes chondrogenesis in rat mesenchymal stem cells: a role for AKT and hypoxia-inducible factor (HIF)-1alpha
    Manoj Kanichai
    Department of Physiology, Trinity College Institute of Neuroscience, Trinity College, Dublin, Ireland
    J Cell Physiol 216:708-15. 2008
    ..To assess the role of HIF-1alpha in the hypoxia-induced increase in chondrogenesis, we employed an siRNA knockdown approach...
  40. ncbi Chondrogenic differentiation of mesenchymal stem cells from bone marrow: differentiation-dependent gene expression of matrix components
    F Barry
    Osiris Therapeutics, Inc, 2001 Aliceanna Street, Baltimore, Maryland 21231, USA
    Exp Cell Res 268:189-200. 2001
    Transforming growth factor (TGF)-beta-induced chondrogenesis of mesenchymal stem cells derived from bone marrow involves the rapid deposition of a cartilage-specific extracellular matrix...
  41. ncbi Regulatory mechanisms in the pathways of cartilage and bone formation
    B de Crombrugghe
    The University of Texas M D Anderson Cancer Center, Department of Molecular Genetics, 1515 Holcombe Boulevard, Box 11, Houston, Texas 77030, USA
    Curr Opin Cell Biol 13:721-7. 2001
  42. ncbi Cartilage repair using bone morphogenetic protein 4 and muscle-derived stem cells
    Ryosuke Kuroda
    Children s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Arthritis Rheum 54:433-42. 2006
  43. ncbi Side population cells isolated from porcine dental pulp tissue with self-renewal and multipotency for dentinogenesis, chondrogenesis, adipogenesis, and neurogenesis
    Koichiro Iohara
    Laboratory of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Aichi, Japan
    Stem Cells 24:2493-503. 2006
    ..Thus, adult pulp contains SP cells, which are enriched for stem cell properties and useful for cell therapy with BMP2 for dentin regeneration...
  44. pmc Granulin epithelin precursor: a bone morphogenic protein 2-inducible growth factor that activates Erk1/2 signaling and JunB transcription factor in chondrogenesis
    Jian Q Feng
    Department of Biomedical Sciences, Baylor College of Dentistry, Texas A and M University System Health Science Center, Dallas, Texas, USA
    FASEB J 24:1879-92. 2010
    ..Collectively, these findings reveal a novel critical role of GEP growth factor in chondrocyte differentiation and the molecular events both in vivo and in vitro...
  45. pmc Chondrogenesis of mesenchymal stem cells: role of tissue source and inducing factors
    Stephane Boeuf
    Research Centre for Experimental Orthopaedics, Orthopaedic University Hospital Heidelberg, Schlierbacher Landstrasse 200a, 69118 Heidelberg, Germany
    Stem Cell Res Ther 1:31. 2010
    ..Although their therapeutic potential is clear, the requirements and conditions for effective induction of chondrogenesis in MSCs and for the production of a stable cartilaginous tissue by these cells are far from being understood...
  46. ncbi Sox9, a key transcription factor of bone morphogenetic protein-2-induced chondrogenesis, is activated through BMP pathway and a CCAAT box in the proximal promoter
    Qiuhui Pan
    Medical Research Center, The Second Affiliated Hospital, Sun Yat Sen University, Guangzhou City, Guangdong Province, PR China
    J Cell Physiol 217:228-41. 2008
    ..The expression of Sox9, a critical transcription factor for the multiple steps of chondrogenesis, has been reported to be upregulated during this process...
  47. ncbi Enhanced early chondrogenesis in articular defects following arthroscopic mesenchymal stem cell implantation in an equine model
    Markus M Wilke
    Comparative Orthopaedics Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, C3 187, Ithaca, New York 14853, USA
    J Orthop Res 25:913-25. 2007
    ..Mesenchymal stem cell grafts improved the early healing response, but did not significantly enhance the long-term histologic appearance or biochemical composition of full-thickness cartilage lesions...
  48. ncbi Identification of markers to characterize and sort human articular chondrocytes with enhanced in vitro chondrogenic capacity
    Shawn Patrick Grogan
    University of Bern, Bern, Switzerland
    Arthritis Rheum 56:586-95. 2007
    ..To identify markers associated with the chondrogenic capacity of expanded human articular chondrocytes and to use these markers for sorting of more highly chondrogenic subpopulations...
  49. ncbi Enhanced chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells in low oxygen environment micropellet cultures
    Brandon D Markway
    Tissue Engineering and Microfluidics Laboratory, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Australia
    Cell Transplant 19:29-42. 2010
    b>Chondrogenesis of mesenchymal stem cells (MSCs) is typically induced when they are condensed into a single aggregate and exposed to transforming growth factor-beta (TGF-beta)...
  50. ncbi Impact of growth factors and PTHrP on early and late chondrogenic differentiation of human mesenchymal stem cells
    S Weiss
    Division of Experimental Orthopaedics, Orthopaedic University Hospital Heidelberg, Heidelberg, Germany
    J Cell Physiol 223:84-93. 2010
    Common in vitro protocols for chondrogenesis of mesenchymal stem cells (MSCs) induce an inadequate, hypertrophic differentiation cascade reminiscent of endochondral bone formation...
  51. ncbi Multilineage mesenchymal differentiation potential of human trabecular bone-derived cells
    Ulrich Nöth
    Departmnent of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, USA
    J Orthop Res 20:1060-9. 2002
    ..These osteoblastic cells have also been shown to undergo adipogenesis in vitro and chondrogenesis in vivo...
  52. ncbi Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow
    Stan Gronthos
    Mesenchymal Stem Cell Group, Division of Haematology, Institute of Medical and Veterinary Science, Adelaide, South Australia, Australia
    J Cell Sci 116:1827-35. 2003
    ..Our novel selection protocol provides a means to generate purified populations of BMSSCs for use in a range of different tissue engineering and gene therapy strategies...
  53. pmc Interleukin-1beta and tumor necrosis factor alpha inhibit chondrogenesis by human mesenchymal stem cells through NF-kappaB-dependent pathways
    N Wehling
    Campus Grosshadern, Ludwig Maximilians University, Munich, Germany
    Arthritis Rheum 60:801-12. 2009
    ..Under clinical conditions, chondrogenesis will often need to occur in the presence of mediators of inflammation produced in response to injury or ..
  54. pmc Expression of transforming growth factor-beta (TGFbeta) and the TGFbeta signalling molecule SMAD-2P in spontaneous and instability-induced osteoarthritis: role in cartilage degradation, chondrogenesis and osteophyte formation
    E N Blaney Davidson
    Experimental Rheumatology and Advanced Therapeutics, Radboud University Medical Centre, Geert Grooteplein 26 28, 6525 GA Nijmegen, The Netherlands
    Ann Rheum Dis 65:1414-21. 2006
    ..In addition, osteophytes can frequently be observed. Transforming growth factor-beta (TGFbeta) has been suggested to be associated with protection against cartilage damage and new cartilage formation as seen in osteophytes...
  55. ncbi BMP-2 and BMP-9 promotes chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1
    M K Majumdar
    Genetics Institute, Inc, Cambridge, Massachusetts 02140, USA
    J Cell Physiol 189:275-84. 2001
    ..human multipotential mesenchymal cells as a target to analyze the effect of bone morphogenetic proteins on chondrogenesis. We also analyzed the effect of proinflammatory cytokine interleukin-1 on chondrogenic-differentiated cells ..
  56. ncbi Fibroblast growth factor-18 stimulates chondrogenesis and cartilage repair in a rat model of injury-induced osteoarthritis
    E E Moore
    ZymoGenetics, Inc, 1201 Eastlake Avenue East, Seattle, WA 98102, USA
    Osteoarthritis Cartilage 13:623-31. 2005
    ..Fibroblast growth factor-18 (FGF18) has been reported to have significant anabolic effects on cartilage. We therefore examined its effects on repair of cartilage damage in a rat meniscal tear model of OA...
  57. ncbi Comparison of mesenchymal tissues-derived stem cells for in vivo chondrogenesis: suitable conditions for cell therapy of cartilage defects in rabbit
    Hideyuki Koga
    Section of Orthopedic Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan
    Cell Tissue Res 333:207-15. 2008
    ..Toxicity of the tracer DiI for in vitro chondrogenesis was also examined...
  58. ncbi Cytokine-like 1 (Cytl1) regulates the chondrogenesis of mesenchymal cells
    Jae Sung Kim
    Department of Life Sciences, Research Center for Biomolecular Nanotechnology, Gwangju Institute of Science and Technology, Gwangju 500 712, Korea
    J Biol Chem 282:29359-67. 2007
    To identify novel molecules regulating chondrogenesis and cartilage development, we screened a cartilage-specific expressed sequence tag data base...
  59. pmc Transcriptional coactivator PGC-1alpha regulates chondrogenesis via association with Sox9
    Yasuhiko Kawakami
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 102:2414-9. 2005
    b>Chondrogenesis is a multistep pathway in which multipotential mesenchymal stem cells (MSC) differentiate into chondrocytes...
  60. pmc Sox9 directly promotes Bapx1 gene expression to repress Runx2 in chondrocytes
    Satoshi Yamashita
    Department of Systems Biomedicine, National Research Institute of Child Health and Development, Tokyo 157 8535, Japan
    Exp Cell Res 315:2231-40. 2009
    ..Group (HMG) box containing gene 9 (Sox9), plays a critical role in cartilage development by initiating chondrogenesis and preventing the subsequent maturation process called chondrocyte hypertrophy...
  61. ncbi Control of chondrogenesis by the transcription factor Sox9
    Haruhiko Akiyama
    Department of Orthopaedics, Graduate School of Medicine, Kyoto University, 54 Kawahara cho, Shogoin, Sakyo ku, Kyoto, Japan
    Mod Rheumatol 18:213-9. 2008
    ..This review discusses the molecular properties and mechanisms of Sry-related high-mobility-group box (Sox) transcription factor, Sox9, in chondrogenesis.
  62. pmc Growth differentiation factor-5 enhances in vitro mesenchymal stromal cell chondrogenesis and hypertrophy
    Cynthia M Coleman
    Regenerative Medicine Institute, National University of Ireland Galway, Galway City, Ireland
    Stem Cells Dev 22:1968-76. 2013
    ..Together, these data support the application of GDF-5 to enhance MSC chondrogenic differentiation and hypertrophy as a precursor to EO...
  63. ncbi A bioresponsive hydrogel tuned to chondrogenesis of human mesenchymal stem cells
    Chelsea S Bahney
    Department of Orthopaedics and Rehabilitation, Oregon Health and Science University, Portland, Oregon 97239, USA
    FASEB J 25:1486-96. 2011
    ..Furthermore, these changes translated into an increased dynamic compressive modulus. This work presents a practical strategy for designing biomaterials uniquely tuned to individual biological processes...
  64. ncbi Repair of osteochondral defects with hyaluronan- and polyester-based scaffolds
    Luis A Solchaga
    Department of Orthopaedics, Case Western Reserve University School of Medicine and University Hospitals of Cleveland, Cleveland, OH, USA
    Osteoarthritis Cartilage 13:297-309. 2005
  65. pmc The fibrodysplasia ossificans progressiva R206H ACVR1 mutation activates BMP-independent chondrogenesis and zebrafish embryo ventralization
    Qi Shen
    Department of Orthopaedic Surgery, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6081, USA
    J Clin Invest 119:3462-72. 2009
    ..that mutant R206H ACVR1 activated BMP signaling in the absence of BMP ligand and mediated BMP-independent chondrogenesis that was enhanced by BMP...
  66. ncbi Poly-L-lactic acid/hydroxyapatite electrospun nanocomposites induce chondrogenic differentiation of human MSC
    Cristiano Spadaccio
    Cardiac and Molecular Biology Laboratory, Heart, Lung and Esophageal Surgery Institute, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
    Ann Biomed Eng 37:1376-89. 2009
    ..This nanocomposite could be an amenable alternative scaffold for cartilage tissue engineering using hMSCs...
  67. ncbi Population doublings and percentage of S100-positive cells as predictors of in vitro chondrogenicity of expanded human articular chondrocytes
    Samoa Giovannini
    Institute of Pathology, Bern, Switzerland
    J Cell Physiol 222:411-20. 2010
    ..Although these data further validated PD as critical for in vitro chondrogenesis, due to the early shift in expression, CD90 could not be considered for predicting chondrogenic potential of ..
  68. ncbi Dexamethasone inhibits insulin-induced chondrogenesis of ATDC5 cells by preventing PI3K-Akt signaling and DNA binding of Runx2
    Takashi Fujita
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, 573 0101, Japan
    J Cell Biochem 93:374-83. 2004
    ..In this study, we investigated the effect of application of dexamethasone (DEX) at the early stage of chondrogenesis using the prechondrogenic cell line, ATDC5, which differentiates into chondrocytes in the presence of insulin...
  69. ncbi Cell specific differences between human adipose-derived and mesenchymal-stromal cells despite similar differentiation potentials
    Daniele Noel
    INSERM U844, Montpellier, F 34091 France
    Exp Cell Res 314:1575-84. 2008
    ..In summary, our observations indicate that ADSC and MSC are fundamentally different cell types and differently committed cells...
  70. pmc Transglutaminase 2 regulates early chondrogenesis and glycosaminoglycan synthesis
    Dmitry Nurminsky
    Dept Biochemistry and Molecular Biology, University of Maryland, School of Medicine, Baltimore, MD 21201, USA
    Mech Dev 128:234-45. 2011
    ..We analyzed the role of TG2 in early stages of chondrogenesis using differentiating high-density cultures of mesenchymal cells from chicken limb bud as a model...
  71. ncbi Dual role of the basic helix-loop-helix transcription factor scleraxis in mesoderm formation and chondrogenesis during mouse embryogenesis
    D Brown
    Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235 9148, USA
    Development 126:4317-29. 1999
    ..These results reveal an essential early role for scleraxis in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages, and suggest that scleraxis target genes mediate these processes...
  72. ncbi Fibrochondrogenesis in two embryonic stem cell lines: effects of differentiation timelines
    Gwendolyn M Hoben
    Rice University, Department of Bioengineering, MS 142, P O Box 1892, Houston, Texas 77251 1892, USA
    Stem Cells 26:422-30. 2008
  73. ncbi Hypoxia impedes hypertrophic chondrogenesis of human multipotent stromal cells
    Debby Gawlitta
    Department of Orthopaedics, University Medical Center Utrecht, Utrecht, The Netherlands
    Tissue Eng Part A 18:1957-66. 2012
    ..This finding has implications for cartilage tissue engineering as well as for endochondral bone tissue engineering, as these approaches deal with, respectively, the inhibition or enhancement of hypertrophic chondrogenesis.
  74. ncbi ATP oscillations mediate inductive action of FGF and Shh signalling on prechondrogenic condensation
    Hyuck Joon Kwon
    Regenerative Medicine Tissue Engineering Division, Research Center for Cooperative Projects, Graduate School of Medicine, Hokkaido University, N 15 W 7, Kita ku, Sapporo, 060 8638, Japan
    Cell Biochem Funct 31:75-81. 2013
    ..These results indicate that ATP oscillations mediate the actions of FGF and Shh signalling on prechondrogenic condensation. This study proposes that morphogens organize skeletal patterns via ATP oscillations...
  75. ncbi Shox2 regulates progression through chondrogenesis in the mouse proximal limb
    Brent E Bobick
    Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta T2N 1N4, Canada
    J Cell Sci 125:6071-83. 2012
    ..Shox2 deletion in undifferentiated mesenchymal cells results in increased BMP activity that enhances early chondrogenesis, but is insufficient to provoke chondrocyte maturation and hypertrophy...
  76. pmc FGF9 regulates early hypertrophic chondrocyte differentiation and skeletal vascularization in the developing stylopod
    Irene H Hung
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, Campus Box 8103, 660 S Euclid Avenue, St Louis, MO 63110, USA
    Dev Biol 307:300-13. 2007
    ..In developing stylopod elements, FGF9 promotes chondrocyte hypertrophy at early stages and regulates vascularization of the growth plate and osteogenesis at later stages of skeletal development...
  77. pmc Development and characterization of a clinically compliant xeno-free culture medium in good manufacturing practice for human multipotent mesenchymal stem cells
    Lucas G Chase
    Primary and Stem Cell Systems, Life Technologies, Madison, Wisconsin, USA
    Stem Cells Transl Med 1:750-8. 2012
  78. pmc MicroRNA-221 regulates chondrogenic differentiation through promoting proteosomal degradation of slug by targeting Mdm2
    Dongkyun Kim
    Department of Biological Sciences, College of Natural Sciences, Wonkwang University, Iksan, Chunbuk 570 749, Korea
    J Biol Chem 285:26900-7. 2010
    ..We determined that mdm2 is a relevant target of miR-221 during chondrogenesis. miR-221 was necessary and sufficient to down-regulate Mdm2 expression, and this down-modulation of Mdm2 by ..
  79. ncbi Characterization and differentiation of equine tendon-derived progenitor cells
    A B Lovati
    Universita degli Studi di Milano, Department of Veterinary Clinical Science, Lodi, Italy
    J Biol Regul Homeost Agents 25:S75-84. 2011
    ..These findings suggest that TSPCs may represent a good model for stem cell biology and could be useful for future tendon regenerative medicine investigations...
  80. ncbi Secretory phospholipase A2 promotes MMP-9-mediated cell death by degrading type I collagen via the ERK pathway at an early stage of chondrogenesis
    Young Ae Choi
    Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu 702 701, Korea
    Biol Cell 102:107-19. 2010
    ..Even though ECM remodelling is an essential step for chondrogenesis, the expression and functions of sPLA2 during chondrogenesis have not been studied.
  81. pmc Fibronectin and stem cell differentiation - lessons from chondrogenesis
    Purva Singh
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    J Cell Sci 125:3703-12. 2012
    ..Finally, we discuss data that support the possibility that the fibronectin matrix has an instructive role in directing cells through the condensation, proliferation and/or differentiation stages of cartilage formation...
  82. ncbi Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure
    M J Oedayrajsingh-Varma
    Department of Plastic Reconstructive and Hand Surgery, VU University Medical Center, Amsterdam, The Netherlands
    Cytotherapy 8:166-77. 2006
    ..We studied whether the yield and growth characteristics of ASC were affected by the type of surgical procedure used for adipose tissue harvesting, i.e. resection, tumescent liposuction and ultrasound-assisted liposuction...
  83. ncbi Canonical Wnt/beta-catenin signaling prevents osteoblasts from differentiating into chondrocytes
    Theo P Hill
    Research Institute for Molecular Pathology, Vienna, Austria
    Dev Cell 8:727-38. 2005
    ..Thus, canonical Wnt/beta-catenin signaling is essential for skeletal lineage differentiation, preventing transdifferentiation of osteoblastic cells into chondrocytes...
  84. ncbi Detailed examination of cartilage formation and endochondral ossification using human mesenchymal stem cells
    Shizuko Ichinose
    Instrumental Analysis Research Center, Tokyo Medical and Dental University, Japan
    Clin Exp Pharmacol Physiol 32:561-70. 2005
    ..2. Human mesenchymal stem cells (hMSC) were maintained in alginate beads with a chondrogenesis-induction medium containing 10 ng/mL transforming growth factor (TGF)-beta3. 3...
  85. ncbi Differential effects of hypoxia on osteochondrogenic potential of human adipose-derived stem cells
    Christophe Merceron
    Institut National de la Santé et de la Recherche Médicale U791, Laboratoire d Ingénierie Ostéo Articulaire et Dentaire LIOAD, Group Physiopathology of Skeletal Tissues and Cartilage Engineering, 44042 Nantes Cedex 1, France
    Am J Physiol Cell Physiol 298:C355-64. 2010
    ..These data could help us exploit the potential of tissue engineering and stem cells to replace or restore the function of osteoarticular tissues...
  86. pmc MiR-365: a mechanosensitive microRNA stimulates chondrocyte differentiation through targeting histone deacetylase 4
    Ying jie Guan
    Cell and Molecular Biology Laboratory, Department of Orthopaedics, Alpert Medical School of Brown University Rhode Island Hospital, 1 Hoppin St, Ste 402, Providence, RI 02903, USA
    FASEB J 25:4457-66. 2011
    ..Taken together, miR-365 is the first identified mechanically responsive microRNA that regulates chondrocyte differentiation via directly targeting HDAC4...
  87. ncbi Alterations in the spatiotemporal expression pattern and function of N-cadherin inhibit cellular condensation and chondrogenesis of limb mesenchymal cells in vitro
    Anthony M DeLise
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Cell Biochem 87:342-59. 2002
    ..and its mutant forms on cellular condensation, on the basis of peanut agglutinin (DNA) staining, and chondrogenesis, based on expression of chondrocyte phenotypic markers, were characterized...
  88. pmc Effects of plating density and culture time on bone marrow stromal cell characteristics
    Birgit Neuhuber
    Drexel University College of Medicine, Department of Neurobiology and Anatomy, Philadelphia, PA, USA
    Exp Hematol 36:1176-85. 2008
    ..This study is aimed at evaluating the effects of initial plating density and total time in culture on proliferation, cell morphology, and differentiation potential of heterogeneous MSC cultures and more homogeneous cloned subpopulations...
  89. ncbi Enhanced chondrocyte proliferation and mesenchymal stromal cells chondrogenesis in coculture pellets mediate improved cartilage formation
    Chitrangada Acharya
    Department of Surgery, University Hospital Basel, Basel, Switzerland
    J Cell Physiol 227:88-97. 2012
    ..the interactions between primary chondrocytes and mesenchymal stem/stromal cells (MSC) accounting for improved chondrogenesis in coculture systems...
  90. ncbi Regional multilineage differentiation potential of meniscal fibrochondrocytes: implications for meniscus repair
    Robert L Mauck
    Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892, USA
    Anat Rec (Hoboken) 290:48-58. 2007
    ..MFCs from all regions of the meniscus possess a multilineage differentiation capability, particularly toward chondrogenesis and adipogenesis...
  91. pmc SOX9 governs differentiation stage-specific gene expression in growth plate chondrocytes via direct concomitant transactivation and repression
    Victor Y L Leung
    Department of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
    PLoS Genet 7:e1002356. 2011
    Cartilage and endochondral bone development require SOX9 activity to regulate chondrogenesis, chondrocyte proliferation, and transition to a non-mitotic hypertrophic state...
  92. ncbi Bone and cartilage formation by skeletal muscle derived cells
    M Mastrogiacomo
    Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy
    J Cell Physiol 204:594-603. 2005
    ..In this respect SMDCs resemble bone marrow stromal cells (BMSCs)...
  93. ncbi Comparative review of growth factors for induction of three-dimensional in vitro chondrogenesis in human mesenchymal stem cells isolated from bone marrow and adipose tissue
    Jennifer L Puetzer
    Joint Department of Biomedical Engineering at UNC Chapel Hill and North Carolina State University, Raleigh, North Carolina 27695, USA
    Tissue Eng Part B Rev 16:435-44. 2010
    ..To date, the most promising growth factors for chondrogenesis appear to be TGFbeta-3 and bone morphogenetic protein (BMP)-6...
  94. ncbi Improving chondrogenesis: potential and limitations of SOX9 gene transfer and mechanical stimulation for cartilage tissue engineering
    Laszlo Kupcsik
    AO Research Institute, Davos Platz, Switzerland
    Tissue Eng Part A 16:1845-55. 2010
    ..We attempted to achieve stable chondrogenesis without any requirement for exogenous growth factors...
  95. ncbi Treatment of chondral defects of the knee with one step matrix-assisted technique enhanced by autologous concentrated bone marrow: in vitro characterisation of mesenchymal stem cells from iliac crest and subchondral bone
    Laura de Girolamo
    Orthopaedic Biotechnologies Lab, IRCCS Galeazzi Orthopaedic Institute, Milan, Italy
    Injury 41:1172-7. 2010
    ..The Autologous Matrix-Induced Chondrogenesis technique (AMIC(®)) combines the micro-fracture procedure with the use of a specific biological membrane...
  96. ncbi Distinct stem cells subpopulations isolated from human adipose tissue exhibit different chondrogenic and osteogenic differentiation potential
    Tommaso Rada
    3B s Research Group Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, 4806 909 Taipas, Guimaraes, Portugal
    Stem Cell Rev 7:64-76. 2011
    ..Furthermore, the results obtained clearly evidence of the advantages of using selected populations in cell-based therapies, such as bone and cartilage regenerative medicine approaches...
  97. pmc Rescue of impaired fracture healing in COX-2-/- mice via activation of prostaglandin E2 receptor subtype 4
    Chao Xie
    The Center for Musculoskeletal Research, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Am J Pathol 175:772-85. 2009
    ..roles of EP2 and EP4 receptors on COX-2-mediated fracture repair, the effects of selective EP agonists on chondrogenesis were examined in E11.5 long-term limb bud micromass cultures...
  98. ncbi MEK/ERK and p38 MAPK regulate chondrogenesis of rat bone marrow mesenchymal stem cells through delicate interaction with TGF-beta1/Smads pathway
    J Li
    State Key Laboratory of Oral Diseases, West China College of Stomatology, West China Hospital of Stomatology, Sichuan University, Chengdu, China
    Cell Prolif 43:333-43. 2010
    This study was carried out to reveal functions and mechanisms of MEK/ERK and p38 pathways in chondrogenesis of rat bone marrow mesenchymal stem cells (BMSCs), and to investigate further any interactions between the mitogen-activated ..
  99. pmc Heparanase expression and activity influences chondrogenic and osteogenic processes during endochondral bone formation
    A J Brown
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    Bone 43:689-99. 2008
    ..To study this, we used high density cultures of ATDC5 pre-chondrogenic cells grown under conditions favoring chondrogenesis or osteogenesis...
  100. ncbi Regulation of chondrocyte differentiation by ADAMTS-12 metalloproteinase depends on its enzymatic activity
    X H Bai
    Institute of Pathogenic Biology, Shandong University School of Medicine, Jinan, P R China
    Cell Mol Life Sci 66:667-80. 2009
    ADAMTS-12, a metalloproteinase that belongs to ADAMTS family, is strongly upregulated during chondrogenesis and demonstrates prominent expression in the growth plate chondrocytes...
  101. pmc Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2
    Wasim S Khan
    Faculty of Life Sciences, United Kingdom Centre for Tissue Engineering at the Wellcome Trust Centre for Cell Matrix Research, Michael Smith Building, University of Manchester, Oxford Road, Manchester, UK
    Arthritis Res Ther 10:R74. 2008
    ..The effects of the expansion of these cells in fibroblast growth factor-2 (FGF-2) were tested on their proliferation, characterisation, and chondrogenic potential...

Research Grants67

  1. BBP (Bone Morphogenetic Protein Binding Peptide) and Bone Healing
    SAMUEL SCOTT MURRAY; Fiscal Year: 2013
    ..Aim 4, we will determine the effects of spp24 degradation on TGF-b-induced intramembranous bone formation and chondrogenesis by quantitative histology and immunohistology of types I and II collagen and osteocalcin...
  2. Novel Collagen II Alternative Transcripts and Mouse Skeletal Development
    Audrey McAlinden; Fiscal Year: 2010
    ..We recently discovered two additional Col2a1 isoforms during in vitro chondrogenesis assays that we have named IIC and IID...
  3. Heat Shock Effects on Human Mesenchymal Stem Cell Differentiation in Hydrogel
    Sihong Wang; Fiscal Year: 2010
    ..on 3D PuraMatrix culture versus conventional 2D culture for osteogenesis or 3D pellet culture for chondrogenesis, using classic differentiation inducers;and (2) to explore dynamic heat-shock effects on hMSC proliferation ..
  4. Identification of Mechanoresponsive Promoter Elements in Chondrogenesis
    Dominik R Haudenschild; Fiscal Year: 2013
  5. Identification of the transcriptional regulators of chondrocyte hypertrophy
    ANDREW BRUCE LASSAR; Fiscal Year: 2013
    ..activation of this enhancer in chicken sternal chondrocytes, (4) that while FoxA1 and FoxA2 are induced during chondrogenesis in micromass cultures of chicken limb bud mesenchymal cells, FoxA2 and FoxA3 are specifically expressed during ..
  6. BMP2 signaling of the development of osetoarthritis
    Vicki Rosen; Fiscal Year: 2013
    ..BMP activity is necessary for initiating chondrogenesis during skeletal development and is required for formation of synovial joints (5-11)...
  7. Function and Regulation of CD-RAP
    Linda J Sandell; Fiscal Year: 2013
    ..It is a compact gene and co-expressed with type IIB collagen in chondrogenesis, making it an excellent model for study of chondrocyte-specific gene expression...
  8. Transcriptional Regulation of Indian Hedgehog Expression in Chondrocytes
    Xiangli Yang; Fiscal Year: 2012
    PROJECT SUMMARY/ABSTRACT During endochondral bone formation, osteogenesis and chondrogenesis are linked and highly coordinated. However, the regulatory mechanisms responsible for coordinating osteogenesis and chondrogenesis remain unclear...
  9. Sox9-mediated control of chondrogenesis
    VERONIQUE M LEFEBVRE; Fiscal Year: 2013
    ..for most of these diseases, and this is largely due to incomplete understanding of the mechanisms underlying chondrogenesis. The main goal of this project is to help lift this barrier by increasing knowledge of the action and ..
  10. Hereditary Multiple Exostosis Syndrome: Pathogenesis and Treatment
    MAURIZIO contact PACIFICI; Fiscal Year: 2010
    ..altering the developmental program of perichondrium-associated progenitor cells and inciting ectopic chondrogenesis and exostosis formation (Aim 1)...
  11. Avian Middle Ear Development
    SUSAN CAROLINE CHAPMAN; Fiscal Year: 2012
    ..the gene expression profile of the columella condensation, replacement and persistent cartilage, and timing of chondrogenesis and ossification of the columella using histology and in situ hybridization analysis, 2) determine if the ..
  12. Engineering Developmental Microenvironments: Cartilage Formation and Maturation
    Robert L Mauck; Fiscal Year: 2012
    ..that degrade via both hydrolytic and enzymatic mechanisms will be investigated as 3D networks to promote MSC chondrogenesis and construct maturation...
  13. Molecular Control of MSC differentiation and Bone Formation by KDM4B
    Cun Yu Wang; Fiscal Year: 2013
    ..cells with self-renewal capabilities and multilineage differentiation potentials including osteogenesis, chondrogenesis and adipogenesis...
  14. The role of microRNAs in chondrogenesis and arthritis
    Hiroshi Asahara; Fiscal Year: 2009
    ..The precise patterning of the developing skeletal framework relies on the appropriate control of chondrogenesis, a multistep process during which mesenchymal cells differentiate into chondrocytes...
  15. Mandibular Cartilage Regeneration In Situ by Endogenous Stem Cell Recruitment
    Wendy S Vanden Berg-Foels; Fiscal Year: 2013
    ..My preliminary data show that 1) synovial cells isolated from the rabbit TMJ exhibit chondrogenesis and ostogenesis in vitro;2) TGFa and PDGF-bb induce chemotaxis and FGF-2 induces proliferation by synovial ..
  16. Identification of gene involved in late stage zebrafish craniofacial development
    REBECCA ANN ANDERSON; Fiscal Year: 2013
    ..Defects in the processes of chondrogenesis and/or osteogenesis lead to craniofacial defects and skeletal dysplasias, and many of the genes responsible ..
  17. Tissue Engineering of a Neotrachea
    James E Dennis; Fiscal Year: 2013 optimized for cartilage sheet formation by first testing a bank of growth factors and cytokines using a chondrogenesis aggregate culture assay system...
  18. Pathogenic Mechanisms of Lysosomal Disease
    Richard Steet; Fiscal Year: 2013
    ..In the first aim, we will further elucidate the cellular mechanisms that underlie abnormal chondrogenesis in ML-II zebrafish...
  19. Transglutaminase 2 in cartilage formation
    Maria Nurminskaya; Fiscal Year: 2011
    ..The objective of this proposal is to investigate the mechanism by which TG2 regulates chondrogenesis in vitro. We will test our hypothesis by pursuing three Specific Aims...
  20. The role of microRNAs in chondrogenesis and arthritis
    Hiroshi Asahara; Fiscal Year: 2013
    ..applicant): The precise patterning of the developing skeletal framework relies on the appropriate control of chondrogenesis, a multistep process during which mesenchymal cells differentiate into chondrocytes...
  21. Cartilage Degeneration and Repair By ADAMTSs and Hyaluronan Binding Proteins
    Anna H Plaas; Fiscal Year: 2013
    ..intra-articular injectables (BMP7 and HA), which are currently in clinical use, to block fibrosis and enhance chondrogenesis in murine OA models...
  22. Polycystin function in zebrafish (PA-06-158)
    Iain A Drummond; Fiscal Year: 2013
    ..cystic kidney disease, vascular aneurysm, abdominal wall hernia, pericardial effusion, and defects in chondrogenesis. In this proposal we plan to exploit our ability to generate compound loss of function as well as gain of ..
    Linda Sandell; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Understanding the mechanisms that control chondrogenesis is of major clinical importance for improving tissue engineering strategies that can be applied to repair cartilage during osteoarthritis, for ..
  24. Molecular mechanisms underlying upper airway patterning and tracheomalacia
    Debora Sinner; Fiscal Year: 2013
    ..Sox9, a master regulator of chondrogenesis, is not detected while the expression domain of Smooth Muscle Actin is expanded...
  25. Chondroitin sulfate-fiber hydrogels for cartilage tissue engineering
    Jeannine Coburn; Fiscal Year: 2012 the base material for the electrospun nanofibers because previous research in our lab has shown enhanced chondrogenesis of mesenchymal stem cells in the presence of CS in the form of hydrogels and fiber-hydrogels compared to PEG ..
  26. Self Assembly of Cartilage by Mesenchymal Stem Cells on Porous Chitosan/CaP
    STEVEN HOWARD ELDER; Fiscal Year: 2010
    ..Specific Aim 1 deals just with enhancing chondrogenesis by MSCs in a scaffold-free model...
  27. CTGF in Pancreatic Stellate Cell-Mediated Fibrogenesis
    David R Brigstock; Fiscal Year: 2011
    ..CTGF stimulates vital biological processes such as chondrogenesis, angiogenesis and matrigenesis via its regulation of cell proliferation, migration, transdifferentiation and ..
  28. Driving tissue formation by inductive stem cell sheet technology
    Eben Alsberg; Fiscal Year: 2013
    ..Determine the role of spatial and temporal presentation of chondrogenic factors and cell number on hMSC sheet chondrogenesis;(2) Determine the effects of dynamic compressive mechanical loading and perfusion, separately and in ..
  29. Facilitating cartilage regeneration by heterogeneous progenitor cells
    RYAN MICHAEL PORTER; Fiscal Year: 2013
    ..However, the intra-articular environment can be hostile to chondrogenesis;specifically, inflammation within the injured joint can inhibit tissue regeneration...
  30. A crosslinked cartilage-derived matrix for cartilage tissue engineering
    Bradley T Estes; Fiscal Year: 2012
    ..studies have demonstrated that this porous cartilage-derived matrix (CDM) can support, and even promote, chondrogenesis of both adipose-derived stem cells (ASCs) and mesenchymal stem cells (MSCs), resulting in a tissue that is ..
  31. CCN proteins in cartilage formation and maintenance
    Karen M Lyons; Fiscal Year: 2013
    ..The central hypothesis is that CCNs 1, 2, and 4 have overlapping functions in and are required for chondrogenesis and postnatal maintenance of articular cartilage and nucleus pulposus (NP), and that they mediate their ..
  32. Heparan sulfate-dependent mechanisms of skeletogenesis
    Julianne Huegel; Fiscal Year: 2012
    ..Congenital alterations in such mechanisms due to HS deficiency would cause ectopic chondrogenesis such as that seen in HME...
  33. Transcriptional control of BMP-2 expression in chondrocytes
    MATTHEW C contact STEWART; Fiscal Year: 2010
    ..distant enhancer in the contexts of articular cartilage 'aging', arthritis and during stem cell chondrogenesis. These experiments will test the hypothesis that alterations in chondrocyte BMP-2 expression occur through ..
  34. Tissue engineering application of endochondral ossification for bone regeneration
    CHELSEA SHIELDS BAHNEY; Fiscal Year: 2013
    ..maturation of MSCs in tissue-engineered scaffolds and developed a MMP-7 bioresponsive system tuned to chondrogenesis. Together these aims address an important clinical problem with a translatable technology capable of improving ..
  35. Transcriptional regulation of Sox9 in chondrogenesis
    TIMOTHY JOSEPH MEAD; Fiscal Year: 2012
    ..the transcription factors that bind to and activate the Sox9 enhancer, and to determine their importance in chondrogenesis. Understanding the molecular basis of Sox9 expression in chondrogenesis will identify potential ..
  36. Heparan Sulfate in Skeletal Development and Diseases
    Yu Yamaguchi; Fiscal Year: 2012
    ..To dissect time-dependence and structural specificity of HS function in chondrogenesis. 2. To determine the mechanisms by which HS regulates BMP function during chondrogenesis. 3...
  37. Roles of Inhibitory Smads in Endochondral Bone Formation
    KRISTINE DAVID ESTRADA; Fiscal Year: 2011
    ..project is to understand how intracellular regulation of BMP and TGFbeta/activin signaling by I-Smads controls chondrogenesis as a prerequisite to more efficient strategies utilizing BMPs to achieve cartilage maintenance and repair...
  38. High-Throughput Craniofacial tissue engineering
    ROBERT SAMUEL LANGER; Fiscal Year: 2013
    ..Small animal model will be used to test osteogenesis in a cranial critical sized defect, while chondrogenesis will be evaluated subcutaneously...
    Karen M Lyons; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): BMPs are essential for chondrogenesis and tissue engineering strategies for the treatment of osteoarthritis...
  40. Molecular Analysis of Extracellular Matrix Assembly
    Jean E Schwarzbauer; Fiscal Year: 2013
    ..that FN matrix controls both cell fate and deposition of collagens in a tissue differentiation model, in vitro chondrogenesis. We will manipulate the level of insoluble FN matrix and its stiffness and determine the effects on ..
  41. Collagen Assembly in Tissue-Engineered Cartilage
    Russell J Fernandes; Fiscal Year: 2013 in the fibrillogenesis of the type II/IX/XI collagen fibril in adult mesenchymal stem cells undergoing chondrogenesis. The aim is to use Western blot, ELISA, LC-mass spectrometry, and other advanced methods in protein chemistry ..
  42. Estrogen Receptor Beta Regulation of Mandibular Condylar Growth
    Sunil Wadhwa; Fiscal Year: 2013
    ..The mandibular condylar cartilage remodels in response to external mechanical strains by regulating chondrogenesis and endochondral ossification, in order to achieve a better balance between mechanical stress and the load ..
    Blanche Capel; Fiscal Year: 2010
    ..mice, markers of cell polarity and cell adhesion, and mice carrying mutations in cMaf and Mafb, members of a family shown to be critical for gonadogenesis in Drosophila, and known to interact with SOX9 during chondrogenesis in mammals.
  44. MR Monitoring of Engineered Tissues
    Richard L Magin; Fiscal Year: 2012
    ..can be applied to characterize non-invasively the changes associated with adipogenesis, osteogenesis, and chondrogenesis in tissue-engineered MSC-based constructs...
  45. Cartilage Repair Using Self Assembling Peptide Scaffolds
    Alan J Grodzinsky; Fiscal Year: 2013
    ..functional 3D-microenvironment for BMSCs within self- assembling peptide hydrogel scaffolds can stimulate chondrogenesis and cartilage neotissue integration in vivo...
  46. Generation of human chondroprogenitor cells for cartilage restoration
    Denis Evseenko; Fiscal Year: 2013
    ..Further I propose that studies to identify and characterize the stages of human chondrogenesis are needed to insure successful generation and isolation of identical ChPC from PSC...
    Clifford J Tabin; Fiscal Year: 2013
    ..Project 2 is devoted to defining the regulatory networks involved in chondrogenesis and osteogenesis within the skeletal elements themselves...
  48. Molecular Mechanisms in Arterial Calcification
    Robert A Terkeltaub; Fiscal Year: 2012
    ..b>Chondrogenesis is modulated by the metabolism of GSH. Vanin-1 pantetheinase suppresses GSH synthesis...
  49. Notch Signaling in Cartilage Development
    Matthew J Hilton; Fiscal Year: 2013
    ..propose to test the novel hypothesis that RBPj?-dependent Notch signaling suppresses MPC differentiation and chondrogenesis by interacting with stabilized [unreadable]-catenin to regulate Hes1 and ultimately Sox9, and is later ..
  50. Role of Trps1 in endochondral bone formation
    Dobrawa Napierala; Fiscal Year: 2011
    ..of the effect of the Trps1 deficiency and over-expression on BMP and hedgehog signaling in a cellular model of chondrogenesis. Additionally, we will test the Trps1 and Ihh genetic antagonism using Trps1;Ihh double mutant mice...
  51. Transcriptional Control of Sox9
    VERONIQUE M LEFEBVRE; Fiscal Year: 2013
    ..the chondrogenin factors that mediate the activity of the first enhancer and to determine their roles in chondrogenesis. We anticipate that the results of this work will have high scientific and technological impact...
  52. Protein-based Cartilage Matrices to Promote Mesenchymal Stem Cell Differentiation
    Julie C Liu; Fiscal Year: 2013
    ..To achieve this goal, the objective is to investigate chondrogenesis using a family of protein-based materials in which the biochemical and biophysical properties are decoupled ..
  53. Articular cartilage stem cells
    Brian Johnstone; Fiscal Year: 2013
    ..pathway for these resident stem cells different from MSCs, and seemingly all other stem cells for which chondrogenesis has been tested? We hypothesize that the lineage restriction of articular cartilage stem cells (ACSCs) is ..
    Daniel Carson; Fiscal Year: 2004
    DESCRIPTION (Adapted from the Investigator's Abstract): Embryonic cartilage formation or chondrogenesis is an early process of mesenchymal conversion leading to the formation of skeletal structures...
  55. Stem Cell-Driven Regeneration of the Mandibular Joint
    Jeremy Mao; Fiscal Year: 2006
    ..The regenerative outcome of tissue-engineered mandibular condyles including architecture, chondrogenesis and osteogenesis will be evaluated qualitatively and quantitatively by our published methods such as selective ..
    Zhengmin Huang; Fiscal Year: 2007
    ..Progressive, coordinated chondrogenesis during cartilage development is precisely controlled by gene expression, especially by gene transcription...
  57. BMP Induced Regeneration of an Endochondral Growth Zone in Digit Regeneration
    Ling Yu; Fiscal Year: 2012
    ..that BMP responsive cells at the amputation injury are directly stimulated to proliferate and/or initiate chondrogenesis via the induction of SOX9...
  58. Regulated Osteochondrogenesis of Human Mesenchymal Stem Cells Using Gene Delivery
    Antonios Mikos; Fiscal Year: 2009
    ..scaffolds will be achieved through gene delivery of transcription factors Sox-5, Sox-6, Sox-9 (Sox trio) for chondrogenesis, and Runx-2 for osteogenesis...
  59. Effect of Versican Mutation on Limb Development In Vitro
    ANTHONY CAPEHART; Fiscal Year: 2006
    ..The chondroitin sulfate proteoglycan, versican, has been implicated in limb chondrogenesis, but its precise role in this process has yet to be defined...
  60. High toughness bio-inspired hydrogels for cartilage tissue engineering
    MICHAEL SCOTT DETAMORE; Fiscal Year: 2010
    ..Another major limitation is the inability to provide sufficient biochemical signals for chondrogenesis. Thus we propose a novel modification to the scaffold by incorporating aggrecan into our high toughness IPN...
    Robert Seegmiller; Fiscal Year: 1990
    ..These experiments, while instructive, have their limitations. In mice, a hereditary condition of chondrogenesis affecting the skeletal system is associated with respiratory distress of the newborn...
    SHERRILL ADAMS; Fiscal Year: 2007
    ..This gene displays a complex pattern of regulation during chondrogenesis and in cartilage pathology...
  63. Factors Regulating Inner Ear Specification
    Jean Pierre Saint Jeannet; Fiscal Year: 2010
    ..One member of this family, Sox9, a well- established regulator of chondrogenesis and sex determination, is also one of the earliest genes expressed in the presumptive otic placode...
    SHAWN O DRISCOLL; Fiscal Year: 2004
    ..Although chondrogenesis diminishes with age, it is clear that it is significantly enhanced through the use of mechanical stimuli, such ..
    Julie Glowacki; Fiscal Year: 2000 determine the mechanisms by which an osteoinductive material, demineralized bone powder (DBP), initiates chondrogenesis in human dermal fibroblasts in vitro, and to determine the fate of induced cartilage when implanted in vivo...
  66. Regulation of cell signaling by Col11a1 during craniofacial development in the ze
    JULIA OXFORD; Fiscal Year: 2009
    ..The process of chondrogenesis and osteogenesis are coordinately regulated during skeletal development in all vertebrates, by common ..
    MARK NATHANSON; Fiscal Year: 1990
    ..will investigate these alternative mechanism by: 1) isolating and characterizing HA synthesis during de novo chondrogenesis, 2) determining the ability of each type of isolated HA to bind proteoglycan monomer, and the kinetics of this ..