osteogenesis

Summary

Summary: The process of bone formation. Histogenesis of bone including ossification.

Top Publications

  1. ncbi Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment
    Rowena McBeath
    The Cellular and Molecular Medicine Program, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Dev Cell 6:483-95. 2004
  2. ncbi The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation
    Kazuhisa Nakashima
    Department of Molecular Genetics and Program in Genes and Development, M D Anderson Cancer Center, University of Texas, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Cell 108:17-29. 2002
  3. ncbi Wnt/beta-catenin signaling in mesenchymal progenitors controls osteoblast and chondrocyte differentiation during vertebrate skeletogenesis
    Timothy F Day
    Geneti Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 8:739-50. 2005
  4. ncbi Canonical Wnt signaling in differentiated osteoblasts controls osteoclast differentiation
    Donald A Glass
    Department of Molecular and Human Genetics, Bone Disease Program of Texas, Houston, 77030, USA
    Dev Cell 8:751-64. 2005
  5. ncbi Novel regulators of bone formation: molecular clones and activities
    J M Wozney
    Tissue Growth and Repair Program, Genetics Institute, Inc, Cambridge, MA 02140
    Science 242:1528-34. 1988
  6. ncbi The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder
    Matthew J Dalby
    Centre for Cell Engineering, Joseph Black Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland, UK
    Nat Mater 6:997-1003. 2007
  7. ncbi High bone density due to a mutation in LDL-receptor-related protein 5
    Lynn M Boyden
    Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    N Engl J Med 346:1513-21. 2002
  8. ncbi Porosity of 3D biomaterial scaffolds and osteogenesis
    Vassilis Karageorgiou
    Department of Chemical and Biological Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA
    Biomaterials 26:5474-91. 2005
  9. ncbi Birth and death of bone cells: basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis
    S C Manolagas
    Division of Endocrinology and Metabolism, University of Arkansas for Medical Sciences, Little Rock 72205, USA
    Endocr Rev 21:115-37. 2000
  10. pmc TGF-beta1-induced migration of bone mesenchymal stem cells couples bone resorption with formation
    Yi Tang
    Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Nat Med 15:757-65. 2009

Detail Information

Publications314 found, 100 shown here

  1. ncbi Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment
    Rowena McBeath
    The Cellular and Molecular Medicine Program, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Dev Cell 6:483-95. 2004
    ..hMSCs allowed to adhere, flatten, and spread underwent osteogenesis, while unspread, round cells became adipocytes...
  2. ncbi The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation
    Kazuhisa Nakashima
    Department of Molecular Genetics and Program in Genes and Development, M D Anderson Cancer Center, University of Texas, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Cell 108:17-29. 2002
    ..Thus, Osx acts downstream of Runx2/Cbfa1. Because Osx null preosteoblasts express typical chondrocyte marker genes, we propose that Runx2/Cbfa1-expressing preosteoblasts are still bipotential cells...
  3. ncbi Wnt/beta-catenin signaling in mesenchymal progenitors controls osteoblast and chondrocyte differentiation during vertebrate skeletogenesis
    Timothy F Day
    Geneti Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 8:739-50. 2005
    ..Controlling Wnt/beta-catenin signaling is a common molecular mechanism underlying chondrocyte and osteoblast differentiation and specification of intramembranous and endochondral ossification...
  4. ncbi Canonical Wnt signaling in differentiated osteoblasts controls osteoclast differentiation
    Donald A Glass
    Department of Molecular and Human Genetics, Bone Disease Program of Texas, Houston, 77030, USA
    Dev Cell 8:751-64. 2005
    ....
  5. ncbi Novel regulators of bone formation: molecular clones and activities
    J M Wozney
    Tissue Growth and Repair Program, Genetics Institute, Inc, Cambridge, MA 02140
    Science 242:1528-34. 1988
    ..Two of the encoded proteins (BMP-2A and BMP-3) are new members of the TGF-beta supergene family, while the third, BMP-1, appears to be a novel regulatory molecule...
  6. ncbi The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder
    Matthew J Dalby
    Centre for Cell Engineering, Joseph Black Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland, UK
    Nat Mater 6:997-1003. 2007
    ..In addition, the current studies show that topographically treated MSCs have a distinct differentiation profile compared with those treated with osteogenic media, which has implications for cell therapies...
  7. ncbi High bone density due to a mutation in LDL-receptor-related protein 5
    Lynn M Boyden
    Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    N Engl J Med 346:1513-21. 2002
    ..Loss-of-function mutations in the gene for low-density lipoprotein receptor-related protein 5 (LRP5), which acts in the Wnt signaling pathway, have been shown to cause osteoporosis-pseudoglioma...
  8. ncbi Porosity of 3D biomaterial scaffolds and osteogenesis
    Vassilis Karageorgiou
    Department of Chemical and Biological Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA
    Biomaterials 26:5474-91. 2005
    ..The effect of these morphological features on osteogenesis in vitro and in vivo, as well as relationships to mechanical properties of the scaffolds, are addressed...
  9. ncbi Birth and death of bone cells: basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis
    S C Manolagas
    Division of Endocrinology and Metabolism, University of Arkansas for Medical Sciences, Little Rock 72205, USA
    Endocr Rev 21:115-37. 2000
    ....
  10. pmc TGF-beta1-induced migration of bone mesenchymal stem cells couples bone resorption with formation
    Yi Tang
    Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Nat Med 15:757-65. 2009
    ..Thus, as TGF-beta1 functions to couple bone resorption and formation, modulation of TGF-beta1 activity could be an effective treatment for bone remodeling diseases...
  11. ncbi Osteogenic BMPs promote tumor growth of human osteosarcomas that harbor differentiation defects
    Xiaoji Luo
    Key Laboratory of Diagnostic Medicine designated by the Chinese Ministry of Education, Department of Pediatric Surgery, Children s Hospital of Chongqing Medical University, Chongqing, China
    Lab Invest 88:1264-77. 2008
    ..Thus, identifying potential differentiation defects in OS tumors would allow us to reconstruct the tumorigenic events in osteoprogenitors and to develop rational differentiation therapies for clinical OS management...
  12. pmc Indian hedgehog signaling regulates proliferation and differentiation of chondrocytes and is essential for bone formation
    B St-Jacques
    Harvard University, Department of Molecular and Cellular Biology, Cambridge, Massachusetts 02138 USA
    Genes Dev 13:2072-86. 1999
    ..Together, the results suggest a model in which Ihh coordinates diverse aspects of skeletal morphogenesis through PTHrP-dependent and independent processes...
  13. ncbi Fracture healing as a post-natal developmental process: molecular, spatial, and temporal aspects of its regulation
    Louis C Gerstenfeld
    Department of Orthopaedic Surgery, Orthopaedic Research Laboratory, Boston University Medical Center, Boston, Massachusetts 02118 2526, USA
    J Cell Biochem 88:873-84. 2003
    ....
  14. ncbi Therapeutic approaches to bone diseases
    G A Rodan
    Merck Research Laboratories, West Point, PA 19486, USA St Vincent s Institute of Medical Research, Melbourne 3065, Australia
    Science 289:1508-14. 2000
    ..Far less attention has been paid to promoting bone formation with, for example, growth factors or hormones, an approach that would be a valuable adjunct therapy for patients receiving inhibitors of bone resorption...
  15. ncbi OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis
    Y Y Kong
    Amgen Institute, Department of Medical Biophysics, University of Toronto, Ontario, Canada
    Nature 397:315-23. 1999
    ..Thus OPGL is a new regulator of lymph-node organogenesis and lymphocyte development and is an essential osteoclast differentiation factor in vivo...
  16. pmc Mesenchymal stem cell-based tissue regeneration is governed by recipient T lymphocytes via IFN-γ and TNF-α
    Yi Liu
    Center for Craniofacial Molecular Biology, Ostrow School of Dentistry, University of Southern California, Los Angeles, California, USA
    Nat Med 17:1594-601. 2011
    ..These data collectively show a previously unrecognized role of recipient T cells in BMMSC-based tissue engineering...
  17. ncbi Smads, p38 and ERK1/2 are involved in BMP9-induced osteogenic differentiation of C3H10T1/2 mesenchymal stem cells
    Dao Jing Xu
    Key Laboratory of Diagnostic Medicine, Chinese Ministry of Education, Chongqing Medical University, Chongqing, PR China
    BMB Rep 45:247-52. 2012
    ..Also, it is noteworthy that p38 and ERK1/2 may play opposing regulatory roles in mediating BMP9-induced osteogenic differentiation of C3H10T1/2 cells...
  18. pmc Conversion of vascular endothelial cells into multipotent stem-like cells
    Damian Medici
    Division of Matrix Biology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA
    Nat Med 16:1400-6. 2010
    ..These stem-like cells could be triggered to differentiate into osteoblasts, chondrocytes or adipocytes. We suggest that conversion of endothelial cells to stem-like cells may provide a new approach to tissue engineering...
  19. pmc MicroRNA-138 regulates osteogenic differentiation of human stromal (mesenchymal) stem cells in vivo
    Tilde Eskildsen
    Molecular Endocrinology Laboratory, KMEB, Odense University Hospital, University of South Denmark, DK 5000 Odense C, Denmark
    Proc Natl Acad Sci U S A 108:6139-44. 2011
    ..Our findings suggest that pharmacological inhibition of miR-138 by antimiR-138 could represent a therapeutic strategy for enhancing bone formation in vivo...
  20. ncbi Human umbilical cord perivascular (HUCPV) cells: a source of mesenchymal progenitors
    Rahul Sarugaser
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, 4 Taddle Creek Road, Room 407, Toronto, ON M5S 3G9, Canada
    Stem Cells 23:220-9. 2005
    ..We conclude that, due to their rapid doubling time, high frequencies of CFU-F and CFU-O, and high MHC-/- phenotype, HUCPV cells represent a significant source of cells for allogeneic mesenchymal cell-based therapies...
  21. ncbi Adipocyte differentiation of bone marrow-derived mesenchymal stem cells: cross talk with the osteoblastogenic program
    S Muruganandan
    Department of Pharmacology, Dalhousie University, Halifax, Nova Scotia, B3H 1X5, Canada
    Cell Mol Life Sci 66:236-53. 2009
    ..Here, we discuss the molecular regulation of bone marrow adipogenesis with emphasis on signals that interact with osteoblastogenic pathways and highlight the possible therapeutic implications of these interactions...
  22. ncbi Tissue origins and interactions in the mammalian skull vault
    Xiaobing Jiang
    Institute for Genetic Medicine, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    Dev Biol 241:106-16. 2002
    ..These observations provide new perspectives on skull evolution and on human genetic abnormalities of skull growth and ossification...
  23. ncbi Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery
    Q Kang
    Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, IL 60637, USA
    Gene Ther 11:1312-20. 2004
    ..Our findings suggest that BMP-6 and BMP-9 may represent more effective osteogenic factors for bone regeneration...
  24. ncbi Canonical WNT signaling promotes osteogenesis by directly stimulating Runx2 gene expression
    Tripti Gaur
    Department of Cell Biology and the Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0106, USA
    J Biol Chem 280:33132-40. 2005
    ..We propose that WNT/TCF1 signaling, like bone morphogenetic protein/transforming growth factor-beta signaling, activates Runx2 gene expression in mesenchymal cells for the control of osteoblast differentiation and skeletal development...
  25. ncbi c-Fos: a key regulator of osteoclast-macrophage lineage determination and bone remodeling
    A E Grigoriadis
    Research Institute of Molecular Pathology IMP, Vienna, Austria
    Science 266:443-8. 1994
    ..These results identify Fos as a key regulator of osteoclast-macrophage lineage determination in vivo and provide insights into the molecular mechanisms underlying metabolic bone diseases...
  26. ncbi Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche
    Yanming Bi
    Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, US National Institutes of Health, 30 Convent Dr 30 225 MSC 4320, Bethesda, Maryland 20892, USA
    Nat Med 13:1219-27. 2007
    ..Our results, while offering new insights into the biology of tendon cells, may assist in future strategies to treat tendon diseases...
  27. ncbi Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells
    Karin Stenderup
    University Department of Endocrinology and Metabolism, University Hospital of Aarhus, DK 8000 Aarhus C, Denmark
    Bone 33:919-26. 2003
    ..In conclusion, aging is associated with decreased proliferative capacity of osteoprogenitor cells, suggesting that decreased osteoblastic cell number, and not function, leads to age-related decrease in bone formation...
  28. ncbi Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs)
    Hongwei Cheng
    Department of Surgery, The University of Chicago Medical Center, Illinois, USA
    J Bone Joint Surg Am 85:1544-52. 2003
    Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion...
  29. ncbi In vitro osteogenesis assays: influence of the primary cell source on alkaline phosphatase activity and mineralization
    C D Hoemann
    Department of Chemical Engineering, Ecole Polytechnique, Montreal, QC, H3C 3A7, Canada
    Pathol Biol (Paris) 57:318-23. 2009
    In trabecular bone fracture repair in vivo, osteogenesis occurs through endochondral ossification under hypoxic conditions, or through woven bone deposition in the vicinity of blood vessels...
  30. ncbi Regulation of the differentiation of mesenchymal stem cells in vitro and osteogenesis in vivo by microenvironmental modification of titanium alloy surfaces
    Yan Hu
    Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China
    Biomaterials 33:3515-28. 2012
    ..The results indicated that Ti6Al4V coated with biofunctional multilayers was beneficial for osteogenesis and integration of implant/bone...
  31. ncbi Regulation of osteogenic differentiation during skeletal development
    Zhong Liang Deng
    Key Laboratory of Diagnostic Medicine and the Affiliated Hospitals of Chongqing Medical University, Chongqing 400046, China
    Front Biosci 13:2001-21. 2008
    ....
  32. ncbi TAZ, a transcriptional modulator of mesenchymal stem cell differentiation
    Jeong Ho Hong
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02139, USA
    Science 309:1074-8. 2005
    ..These results indicate that TAZ functions as a molecular rheostat that modulates MSC differentiation...
  33. ncbi Regulation of rate of cartilage differentiation by Indian hedgehog and PTH-related protein
    A Vortkamp
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 273:613-22. 1996
    ....
  34. ncbi Distinct roles of bone morphogenetic proteins in osteogenic differentiation of mesenchymal stem cells
    Hue H Luu
    Molecular Oncology Laboratory, Department of Surgery, 5841 South Maryland Avenue, MC 3079, Room J 611, The University of Chicago Medical Center, Chicago, Illinois 60637, USA
    J Orthop Res 25:665-77. 2007
    ..It is conceivable that osteogenic BMPs (i.e., BMP2, 4, 6, 7, and 9) may be used to formulate synergistic pairs among themselves and/or with other less osteogenic BMPs for efficacious bone regeneration in clinical settings...
  35. ncbi Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow
    C Campagnoli
    Department of Maternal and Fetal Medicine, Institute of Reproductive and Developmental Biology, Imperial College School of Medicine, London, United Kingdom
    Blood 98:2396-402. 2001
    ..MSCs similar to those derived from adult bone marrow, fetal liver, and fetal bone marrow circulate in first-trimester human blood and may provide novel targets for in utero cellular and gene therapy...
  36. ncbi Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies
    A Stolzing
    Kroto Research Institute, North Campus, University of Sheffield, UK
    Mech Ageing Dev 129:163-73. 2008
    ..Furthermore, there is an age-related decline in overall BM MSC "fitness" which might lead to problems when using autologous aged MSC for cell-based therapies...
  37. pmc A comprehensive analysis of the dual roles of BMPs in regulating adipogenic and osteogenic differentiation of mesenchymal progenitor cells
    Quan Kang
    Key Laboratory of Diagnostic Medicine designated by the Chinese Ministry of Education, and The Children s Hospital, Chongqing Medical University, Chongqing, People s Republic of China
    Stem Cells Dev 18:545-59. 2009
    ....
  38. pmc MMP-9/gelatinase B is a key regulator of growth plate angiogenesis and apoptosis of hypertrophic chondrocytes
    T H Vu
    Department of Anatomy, University of California, San Francisco 94143, USA
    Cell 93:411-22. 1998
    ..Growth plates from gelatinase B-null mice in culture show a delayed release of an angiogenic activator, establishing a role for this proteinase in controlling angiogenesis...
  39. pmc Functions of RANKL/RANK/OPG in bone modeling and remodeling
    Brendan F Boyce
    Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, 601 Elmwood Avenue, Box 626, Rochester, NY 14642, USA
    Arch Biochem Biophys 473:139-46. 2008
    ..Thus, the relative concentration of RANKL and OPG in bone is a major determinant of bone mass and strength. Here, we review our current understanding of the role of the RANKL/RANK/OPG system in bone modeling and remodeling...
  40. pmc TGFbeta/BMP type I receptors ALK1 and ALK2 are essential for BMP9-induced osteogenic signaling in mesenchymal stem cells
    Jinyong Luo
    Key Laboratory of Diagnostic Medicine designated by Chinese Ministry of Education, The Affiliated Hospitals of Chongqing Medical University, Chongqing 400016, China
    J Biol Chem 285:29588-98. 2010
    ..These findings should further aid us in understanding the molecular mechanism through which BMP9 regulates osteogenic differentiation of MSCs...
  41. pmc Genetic analysis of the roles of BMP2, BMP4, and BMP7 in limb patterning and skeletogenesis
    Amitabha Bandyopadhyay
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Genet 2:e216. 2006
    ..In contrast, we find that the loss of both BMP2 and BMP4 results in a severe impairment of osteogenesis.
  42. ncbi Mechanical stimulation of bone in vivo reduces osteocyte expression of Sost/sclerostin
    Alexander G Robling
    Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    J Biol Chem 283:5866-75. 2008
    ..of sclerostin levels appears to be a finely tuned mechanism by which osteocytes coordinate regional and local osteogenesis in response to increased mechanical stimulation, perhaps via releasing the local inhibition of Wnt/Lrp5 ..
  43. ncbi Multipotential human adipose-derived stromal stem cells exhibit a perivascular phenotype in vitro and in vivo
    A C W Zannettino
    Myeloma Research Program, Bone and Cancer Laboratories, Division of Haematology, Institute of Medical and Veterinary Science, Hanson Institute and University of Adelaide, Adelaide, Australia
    J Cell Physiol 214:413-21. 2008
    ..The findings presented here, describe a multipotential stem cell population within adult human adipose tissue, which appear to be intimately associated with perivascular cells surrounding the blood vessels...
  44. pmc Molecular mechanisms controlling bone formation during fracture healing and distraction osteogenesis
    Z S Ai-Aql
    Orthopaedic Surgical Research Laboratory, Department of Orthopaedic Surgery, Boston University Medical Center, Boston, MA 02118, USA
    J Dent Res 87:107-18. 2008
    Fracture healing and distraction osteogenesis have important applications in orthopedic, maxillofacial, and periodontal treatment...
  45. ncbi Vascular calcification: pathobiological mechanisms and clinical implications
    Rebecca C Johnson
    Cardiovascular Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Circ Res 99:1044-59. 2006
    ..Through greater understanding of both the mechanism and clinical consequences of vascular calcification, future therapeutic strategies may be more effectively designed and applied...
  46. pmc Hey1 basic helix-loop-helix protein plays an important role in mediating BMP9-induced osteogenic differentiation of mesenchymal progenitor cells
    Katie A Sharff
    Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois 60637, USA
    J Biol Chem 284:649-59. 2009
    ..Thus, our findings suggest that Hey1, through its interplay with Runx2, may play an important role in regulating BMP9-induced osteoblast lineage differentiation of MSCs...
  47. ncbi Stimulation of bone formation in vitro and in rodents by statins
    G Mundy
    OsteoScreen, 2040 Babcock Road, San Antonio, TX 78229, USA
    Science 286:1946-9. 1999
    ..Thus, in appropriate doses, statins may have therapeutic applications for the treatment of osteoporosis...
  48. ncbi PTH/PTHrP receptor in early development and Indian hedgehog-regulated bone growth
    B Lanske
    Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Science 273:663-6. 1996
    ..These results suggest that the PTH/PTHrP receptor mediates the effects of Indian Hedgehog and PTHrP on chondrocyte differentiation...
  49. ncbi Costimulatory signals mediated by the ITAM motif cooperate with RANKL for bone homeostasis
    Takako Koga
    Department of Immunology, Graduate School of Medicine and Faculty of Medicine, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113 0033, Japan
    Nature 428:758-63. 2004
    ..These results reveal that RANKL and M-CSF are not sufficient to activate the signals required for osteoclastogenesis...
  50. ncbi FGF signaling pathways in endochondral and intramembranous bone development and human genetic disease
    David M Ornitz
    Department of Molecular Biology and Pharmacology, Washington University Medical School, St Louis, Missouri 63110, USA
    Genes Dev 16:1446-65. 2002
  51. pmc P38 and ERK1/2 MAPKs act in opposition to regulate BMP9-induced osteogenic differentiation of mesenchymal progenitor cells
    Yingze Zhao
    Key Laboratory of Diagnostic Medicine designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China
    PLoS ONE 7:e43383. 2012
    ..What is most noteworthy, however, is that p38 and ERK1/2 act in opposition to regulate BMP9-induced osteogenic differentiation of MPCs...
  52. pmc Multiple functions of Osterix are required for bone growth and homeostasis in postnatal mice
    Xin Zhou
    Department of Genetics, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 107:12919-24. 2010
    ..Our study identifies Osx as an essential multifunctional player in postnatal bone growth and homeostasis...
  53. pmc Upregulation of miR-22 promotes osteogenic differentiation and inhibits adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells by repressing HDAC6 protein expression
    Shan Huang
    Center of Excellence in Tissue Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Institute of Basic Medical Sciences and School of Basic Medicine, Beijing, People s Republic of China
    Stem Cells Dev 21:2531-40. 2012
    ..into either adipocytes or osteoblasts, and a reciprocal relationship exists between adipogenesis and osteogenesis. Multiple transcription factors and signaling pathways have been reported to regulate adipogenic or osteogenic ..
  54. ncbi MicroRNAs regulate osteogenesis and chondrogenesis
    Shiwu Dong
    Laboratory of Biomechanics, Department of Anatomy, The Third Military Medical University, Chongqing, China
    Biochem Biophys Res Commun 418:587-91. 2012
    ..Here, we review the recent research progress on the regulation of miRNAs in bone biology, with a particular focus on the miRNA-mediated control mechanisms of bone and cartilage formation...
  55. ncbi miR-182 is a negative regulator of osteoblast proliferation, differentiation, and skeletogenesis through targeting FoxO1
    Kyoung Min Kim
    Division of Endocrinology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea
    J Bone Miner Res 27:1669-79. 2012
    ..crucial miRNA, miR-182, which regulates osteoblastogenesis by repressing FoxO1 and thereby negatively affecting osteogenesis. Overexpression of miR-182 in osteoblast lineage cells increased cell apoptosis and inhibited osteoblast ..
  56. ncbi Biocompatibility and osteogenesis of biomimetic nano-hydroxyapatite/polyamide composite scaffolds for bone tissue engineering
    Huanan Wang
    Research Center for Nano Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, 610064, PR China
    Biomaterials 28:3338-48. 2007
    ..To investigate the in vivo biocompatibility and osteogenesis of the composite scaffolds, both pure n-HA/PA scaffolds and MSC/scaffold constructs were implanted in rabbit ..
  57. ncbi High levels of β-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway
    Na Liu
    Department of Stomatology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, People s Republic of China
    J Bone Miner Res 26:2082-95. 2011
    ..Given the important role of Wnt signaling in osteogenic differentiation, it is possible that agents that can modify this pathway may be of value in bone regeneration by MSCs in chronic inflammatory microenvironments...
  58. ncbi Reduced oxygen tension attenuates differentiation capacity of human mesenchymal stem cells and prolongs their lifespan
    Christine Fehrer
    Extracellular Matrix Research, Institute for Biomedical Aging Research, Austrian Academy of Sciences, Rennweg 10, A 6020 Innsbruck, Austria
    Aging Cell 6:745-57. 2007
    ..Low oxygen levels in this location provide a milieu that extends cellular lifespan and furthermore is instructive for the stemness of MSC allowing proliferation upon stimulation while suppressing differentiation...
  59. pmc PPARγ: a circadian transcription factor in adipogenesis and osteogenesis
    Masanobu Kawai
    Center for Clinical and Translational Research, Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME 04074 7205, USA
    Nat Rev Endocrinol 6:629-36. 2010
    ..alterations in the bone marrow milieu-that is, increased bone marrow adiposity with suppression of osteogenesis-could partially explain the pathogenesis of drug-induced bone loss...
  60. pmc Role of HIF-1alpha in skeletal development
    Chao Wan
    Department of Orthopaedic Surgery, Johns Hopkins University, School of Medicine, Baltimore, MD, USA
    Ann N Y Acad Sci 1192:322-6. 2010
    Angiogenesis and osteogenesis are tightly coupled during bone development and regeneration. Mesenchymal cells in the developing stroma elicit angiogenic signals to recruit new blood vessels into bone...
  61. ncbi Regulatory mechanisms of osteoblast and osteoclast differentiation
    T Katagiri
    Department of Biochemistry, School of Dentistry, Showa University, Japan
    Oral Dis 8:147-59. 2002
    ..These findings have opened new areas for exploring the molecular mechanisms of osteoblast and osteoclast differentiation...
  62. ncbi Donor variation in the growth properties and osteogenic potential of human marrow stromal cells
    D G Phinney
    Center for Gene Therapy, MCP Hahnemann University, Philadelphia, Pennsylvania 19102, USA
    J Cell Biochem 75:424-36. 1999
    ..Therefore, it appears that cellular heterogeneity produced by the method of harvest is propagated within and among different donor populations during culture expansion in vitro...
  63. ncbi Sclerostin mediates bone response to mechanical unloading through antagonizing Wnt/beta-catenin signaling
    Chuwen Lin
    Bio X Center, Key Laboratory of Developmental Genetics and Neuropsychiatric Diseases, Ministry of Education, Shanghai Jiao Tong University, Shanghai, China
    J Bone Miner Res 24:1651-61. 2009
    ..Our findings also indicated sclerostin is a promising target for preventing disuse osteoporosis...
  64. ncbi Wnt 3a promotes proliferation and suppresses osteogenic differentiation of adult human mesenchymal stem cells
    Genevieve M Boland
    Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis, and Musculoskeletal and Skin Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892 8022, USA
    J Cell Biochem 93:1210-30. 2004
    ..To analyze the role of Wnt signaling in mesenchymal osteogenesis, we have profiled the expression of WNTs and their receptors, FRIZZLEDs (FZDs), and several secreted Wnt ..
  65. ncbi A comparative study of proliferation and osteogenic differentiation of adipose-derived stem cells on akermanite and beta-TCP ceramics
    Qihai Liu
    Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, PR China
    Biomaterials 29:4792-9. 2008
    ..Thus, it can be ascertained that akermanite ceramics may serve as a potential scaffold for bone tissue engineering...
  66. pmc Cellular and molecular mechanisms of bone remodeling
    Liza J Raggatt
    Faculty of Health Sciences, The University of Queensland Centre for Clinical Research, Royal Brisbane Hospital, Herston 4029, Australia
    J Biol Chem 285:25103-8. 2010
    ..Specifically discussed in detail are the cellular and molecular mechanisms of bone remodeling, including events that orchestrate the five sequential phases of bone remodeling: activation, resorption, reversal, formation, and termination...
  67. ncbi Aminobisphosphonates cause osteoblast apoptosis and inhibit bone nodule formation in vitro
    Aymen I Idris
    Rheumatic Diseases Unit, Molecular Medicine Centre, University of Edinburgh, General Western Hospital, Edinburgh EH4 2XU, UK
    Calcif Tissue Int 82:191-201. 2008
    ..This raises the possibility that inhibition of bone formation by bisphosphonates may be due in part to a direct inhibitory effect on cells of the osteoblast lineage...
  68. ncbi Human circulating CD14+ monocytes as a source of progenitors that exhibit mesenchymal cell differentiation
    Masataka Kuwana
    Instiute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan
    J Leukoc Biol 74:833-45. 2003
    ..Our findings represent the first evidence that human circulating CD14+ monocytes are a source of progenitors that exhibit mesenchymal cell differentiation...
  69. ncbi Mesenchymal stem cells: molecular targets for tissue engineering
    Neeraj Kumar Satija
    Stem Cell Gene Therapy Research Group, Institute of Nuclear Medicine and Allied Sciences, Timarpur, Delhi, India
    Stem Cells Dev 16:7-23. 2007
    ..However, a thorough understanding of the molecular mechanism is necessary for enhancing the self-renewal ability and osteogenic potential in vitro...
  70. ncbi Adipose tissue-derived mesenchymal stem cells acquire bone cell-like responsiveness to fluid shear stress on osteogenic stimulation
    Marlene Knippenberg
    Department of Oral Cell Biology, Academic Center of Dentistry Amsterdam, Universiteit van Amsterdam, Amsterdam, The Netherlands
    Tissue Eng 11:1780-8. 2005
    ..ATMSCs might be able to perform bone cell-specific functions during bone (re)modeling in vivo and, therefore, provide a promising new tool for bone tissue engineering...
  71. ncbi Sclerostin is a delayed secreted product of osteocytes that inhibits bone formation
    Kenneth E S Poole
    MRC Bone Research Group, Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge, UK
    FASEB J 19:1842-4. 2005
    ..In doing so, sclerostin may act as a key inhibitory signal governing skeletal microarchitecture...
  72. ncbi Targeted deletion of the sclerostin gene in mice results in increased bone formation and bone strength
    Xiaodong Li
    Department of Metabolic Disorders, Amgen, Thousand Oaks, California 91320 1799, USA
    J Bone Miner Res 23:860-9. 2008
    ..Based on these data, sclerostin has emerged as a key negative regulator of bone mass. We generated SOST knockout (KO) mice to gain a more detailed understanding of the effects of sclerostin deficiency on bone...
  73. ncbi BMP-9 induced osteogenic differentiation of mesenchymal stem cells: molecular mechanism and therapeutic potential
    Gaurav Luther
    Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, IL 60637, USA
    Curr Gene Ther 11:229-40. 2011
    ....
  74. ncbi MicroRNA-100 regulates osteogenic differentiation of human adipose-derived mesenchymal stem cells by targeting BMPR2
    Yang Zeng
    Center of Excellence in Tissue Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Institute of Basic Medical Sciences and School of Basic Medicine, Beijing, People s Republic of China
    FEBS Lett 586:2375-81. 2012
    ..In this study, we examined the role of microRNA (miR)-100 on the osteogenesis of hASCs...
  75. ncbi v-ATPase V0 subunit d2-deficient mice exhibit impaired osteoclast fusion and increased bone formation
    Seoung Hoon Lee
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Nat Med 12:1403-9. 2006
    ....
  76. pmc Mechanical signals as anabolic agents in bone
    Engin Ozcivici
    Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York, NY 11794 2580, USA
    Nat Rev Rheumatol 6:50-9. 2010
    ..Mechanical targeting of the bone marrow stem-cell pool might, therefore, represent a novel, drug-free means of slowing the age-related decline of the musculoskeletal system...
  77. pmc Growth hormone, insulin-like growth factors, and the skeleton
    Andrea Giustina
    Department of Medical and Surgical Sciences, University of Brescia, Brescia, Italy
    Endocr Rev 29:535-59. 2008
    ..GH and IGF-I secretion are decreased in aging individuals, and abnormalities in the GH/IGF-I axis play a role in the pathogenesis of the osteoporosis of anorexia nervosa and after glucocorticoid exposure...
  78. ncbi Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells
    Janne L Simonsen
    Department of Endocrinology and Metabolism, University Hospital of Aarhus, DK 8000 Aarhus C, Denmark
    Nat Biotechnol 20:592-6. 2002
    ..These results suggest that ectopic expression of telomerase in hMSCs prevents senescence-associated impairment of osteoblast functions...
  79. pmc Sonic Hedgehog influences the balance of osteogenesis and adipogenesis in mouse adipose-derived stromal cells
    Aaron W James
    Hagey Pediatric Regenerative Medicine Laboratory, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California 94305 5148, USA
    Tissue Eng Part A 16:2605-16. 2010
    ..Hedgehog signaling, with the Hedgehog antagonist cyclopamine, enhanced adipogenesis at the expense of osteogenesis. We next translated these results to a mouse model of appendicular skeletal regeneration...
  80. ncbi Resveratrol promotes osteogenesis of human mesenchymal stem cells by upregulating RUNX2 gene expression via the SIRT1/FOXO3A axis
    Pei Chi Tseng
    Department of Primary Care Medicine, National Taiwan University College of Medicine, Taipei, Taiwan
    J Bone Miner Res 26:2552-63. 2011
    ..Moreover, SIRT1 antagonism enhances adipogenesis. There has been speculation that resveratrol can promote osteogenesis through SIRT1, but the mechanism remains unclear...
  81. ncbi The CCN family: a new stimulus package
    D R Brigstock
    Department of Surgery, The Ohio State University, Columbus, Ohio 43212, USA
    J Endocrinol 178:169-75. 2003
    ....
  82. ncbi Peri-implant osteogenesis in health and osteoporosis
    Franchi Marco
    Department of Human Anatomical Sciences and Physiopathology of Locomotor Apparatus, Via Irnerio 48, 40136 Bologna, Italy
    Micron 36:630-44. 2005
    ..The peri-implant osteogenesis can proceed from the host bone to the implant surface (distant osteogenesis) and from the implant surface to ..
  83. ncbi Networks and hubs for the transcriptional control of osteoblastogenesis
    Jane B Lian
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Rev Endocr Metab Disord 7:1-16. 2006
    ..Mechanisms by which Runx2 supports commitment to osteogenesis and determines cell fate involve its retention on mitotic chromosomes...
  84. ncbi Characterization of adhesion and differentiation markers of osteogenic marrow stromal cells
    R Marom
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Israel
    J Cell Physiol 202:41-8. 2005
    Marrow stroma cells (MSC) play a major role in osteogenesis. The potential of the MSC to differentiate to bone-forming cells relies upon molecular regulation...
  85. pmc A network connecting Runx2, SATB2, and the miR-23a~27a~24-2 cluster regulates the osteoblast differentiation program
    Mohammad Q Hassan
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 107:19879-84. 2010
    Induced osteogenesis includes a program of microRNAs (miRs) to repress the translation of genes that act as inhibitors of bone formation. How expression of bone-related miRs is regulated remains a compelling question...
  86. pmc The p38 MAPK pathway is essential for skeletogenesis and bone homeostasis in mice
    Matthew B Greenblatt
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, and Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA
    J Clin Invest 120:2457-73. 2010
    ..These results also suggest that selective p38beta agonists may represent attractive therapeutic agents to prevent bone loss associated with osteoporosis and aging...
  87. ncbi Leptin regulation of bone resorption by the sympathetic nervous system and CART
    Florent Elefteriou
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 434:514-20. 2005
    ..Our study establishes that leptin-regulated neural pathways control both aspects of bone remodelling, and demonstrates that integrity of sympathetic signalling is necessary for the increase in bone resorption caused by gonadal failure...
  88. ncbi Senescence-unrelated impediment of osteogenesis from Flk1+ bone marrow mesenchymal stem cells induced by total body irradiation and its contribution to long-term bone and hematopoietic injury
    Jie Ma
    Institute of Basic Medical Sciences and School of Basic Medicine, Center of Excellence in Tissue Engineering, Chinese Acaademy of Medical Sciences and Peking Union Medical College, Beijing, 100005, China
    Haematologica 92:889-96. 2007
    ..The objectives of this study were to determine the response of BM MSC to irradiation stress, such as cellular senescence and differentiation potential in BM MSC, and the clinical significance of these changes caused by TBI...
  89. pmc P2X7 receptors on osteoblasts couple to production of lysophosphatidic acid: a signaling axis promoting osteogenesis
    Nattapon Panupinthu
    Canadian Institutes of Health Research Group in Skeletal Development and Remodeling, London, ON, Canada
    J Cell Biol 181:859-71. 2008
    ..Moreover, osteogenesis is suppressed in calvarial cell cultures from P2rx7-/- mice compared with the wild type...
  90. ncbi Glucocorticoids suppress bone formation by attenuating osteoblast differentiation via the monomeric glucocorticoid receptor
    Alexander Rauch
    Tissue specific hormone action, Leibniz Institute for Age Research, Fritz Lipmann Institute, 07745 Jena, Germany
    Cell Metab 11:517-31. 2010
    ..Thus, GCs inhibit cytokines independent of GR dimerization and thereby attenuate osteoblast differentiation, which accounts, in part, for bone loss during GC therapy...
  91. ncbi Osteomalacia in hyp mice is associated with abnormal phex expression and with altered bone matrix protein expression and deposition
    D Miao
    Department of Medicine, McGill University, Montreal, Quebec, Canada H3A 1A1
    Endocrinology 142:926-39. 2001
    ..These results suggest that alterations in the Phex gene may control bone matrix mineralization indirectly by regulating the synthesis and deposition of bone matrix proteins...
  92. pmc Skeletal cell fate decisions within periosteum and bone marrow during bone regeneration
    Celine Colnot
    Department of Orthopaedic Surgery, University of California at San Francisco, San Francisco General Hospital, San Francisco, California 94110, USA
    J Bone Miner Res 24:274-82. 2009
    ....
  93. pmc Synergistic enhancement of bone formation and healing by stem cell-expressed VEGF and bone morphogenetic protein-4
    Hairong Peng
    Growth and Development Laboratory, Department of Orthopaedic Surgery, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Clin Invest 110:751-9. 2002
    ..This study has important implications for the formulation of new strategies to improve bone healing through increasing mesenchymal stem cell recruitment and survival, in combination with muscle-derived stem cell-based gene therapy...
  94. ncbi SOX9 is a major negative regulator of cartilage vascularization, bone marrow formation and endochondral ossification
    Takako Hattori
    Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama City 700 8525, Japan
    Development 137:901-11. 2010
    ..These findings imply that downregulation of Sox9 in the hypertrophic zone of the normal growth plate is essential for allowing vascular invasion, bone marrow formation and endochondral ossification...
  95. pmc Antibody-based inhibition of DKK1 suppresses tumor-induced bone resorption and multiple myeloma growth in vivo
    Shmuel Yaccoby
    Myeloma Institute for Research and Therapy, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    Blood 109:2106-11. 2007
    ..We conclude that DKK1 is a key player in MM bone disease and that blocking DKK1 activity in myelomatous bones reduces osteolytic bone resorption, increases bone formation, and helps control MM growth...
  96. ncbi Osteogenesis and angiogenesis: the potential for engineering bone
    J M Kanczler
    Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Developmental Origins of Health and Disease, Institute of Developmental Sciences, University of Southampton, Southampton, SO16 6YD
    Eur Cell Mater 15:100-14. 2008
    ..therapies of stem cells and polymeric growth factor release scaffolds tailored to promote angiogenesis and osteogenesis are under evaluation and development actively to stimulate bone regeneration...
  97. ncbi Wnt signaling and osteoblastogenesis
    Peter V N Bodine
    Women s Heath and Musculoskeletal Biology, Wyeth Research, 500 Arcola Road, Collegeville, PA 19426, USA
    Rev Endocr Metab Disord 7:33-9. 2006
    ....
  98. pmc Shear stress induces osteogenic differentiation of human mesenchymal stem cells
    Gregory Yourek
    Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, IL 60612, USA
    Regen Med 5:713-24. 2010
    ..To determine whether fluid flow-induced shear stress affects the differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs) into osteogenic cells...
  99. ncbi Differentiation of pre-osteoblast cells on poly(ethylene terephthalate) grafted with RGD and/or BMPs mimetic peptides
    Omar F Zouani
    INSERM, U577, Biomatériaux et Réparation Tissulaire, Univ Victor Segalen Bordeaux 2, Bordeaux, 146 rue Leo Saignat, 33076 Bordeaux Cedex, France
    Biomaterials 31:8245-53. 2010
    ..These findings are potentially useful in developing engineered biomaterials for bone regeneration...
  100. pmc Mutations in fam20b and xylt1 reveal that cartilage matrix controls timing of endochondral ossification by inhibiting chondrocyte maturation
    B Frank Eames
    Institute of Neuroscience, University of Oregon, Eugene, Oregon, United States of America
    PLoS Genet 7:e1002246. 2011
    ....
  101. ncbi Non-invasive axial loading of mouse tibiae increases cortical bone formation and modifies trabecular organization: a new model to study cortical and cancellous compartments in a single loaded element
    Roberto L de Souza
    Department of Veterinary Basic Sciences, Royal Veterinary College, Royal College Street, London NW1 0TU, UK
    Bone 37:810-8. 2005
    ....

Research Grants78

  1. Novel, Rapidly Translatable Technologies for Healing Long Bone Segmental Defects
    Christopher H Evans; Fiscal Year: 2010
    ..Current approaches to treating such injuries include the use of autograft and allograft bone, distraction osteogenesis, and the application of recombinant, human bone morphogenetic proteins (BMPs)...
  2. BBP (Bone Morphogenetic Protein Binding Peptide) and Bone Healing
    SAMUEL SCOTT MURRAY; Fiscal Year: 2013
    ..slow release/immobilization characteristics to BMP", thus "reducing the amount of BMP needed" to stimulate osteogenesis. DBM contains secreted phosphoprotein-24 kDa (spp24), a proteolytically-labile protein that contains both ..
  3. Heat Shock Effects on Human Mesenchymal Stem Cell Differentiation in Hydrogel
    Sihong Wang; Fiscal Year: 2010
    ..PuraMatrix, and to evaluate hMSC differentiation on 3D PuraMatrix culture versus conventional 2D culture for osteogenesis or 3D pellet culture for chondrogenesis, using classic differentiation inducers;and (2) to explore dynamic heat-..
  4. Hematopoietic Stem Cell Transplantation in Osteogenesis Imperfecta
    Meenal Mehrotra; Fiscal Year: 2012
    ..Long term, these findings have the potential to identify HSC transplantation, and not the transplantation of MSCs or progenitor cells, as the therapy of choice for this collagen disorder. ..
  5. Repression of PPARgamma-2 by Glucocorticoid-Induced Leucine Zipper
    Xing Ming Shi; Fiscal Year: 2012
    ..as the "master" regulator of adipogenesis, positively regulates adipogenesis but has the opposite effect on osteogenesis. Our long-term goal is to determine the molecular mechanisms by which PPAR(2 controls both adipogenic and ..
  6. The Role of FKBP10 in Recessive Osteogenesis Imperfecta
    Caressa Lietman; Fiscal Year: 2013
    b>Osteogenesis Imperfecta (OI) is the most commonly inherited form of brittle bone disease and displays a spectrum of severity from mild phenotypes to severe early lethality...
  7. Shared Mechanisms of Valvular and Vascular Calcification
    JOEL DAVID MORRISETT; Fiscal Year: 2012
    ..and primary mitral valve disease (myxomatous) involve upregulation of chondrogenic genes, but progression to osteogenesis only occurs in the aortic valve, not in the mitral valve...
  8. Effect of Polybrominated Diphenal Ether Flame Retardant Exposure on Osteogenesis
    Deena J Small; Fiscal Year: 2010
    ..The first aim to test this hypothesis is to determine if PBDE impairs osteogenesis and/or osteoblast function by antagonizing thyroid hormone receptor-mediated gene expression...
  9. 3D Differentiation of Mesenchymal Stem Cells in Peptide Amphiphile Matrices
    John B Matson; Fiscal Year: 2011
    ..Dex is well-known to induce osteogenesis of hMSCs in 2-dimensional culture, but an injectable, 3-dimensional, Dex-releasing gel capable of ..
  10. Effect of Smoking on Adult Stem Cell Differentiation
    Herman S Cheung; Fiscal Year: 2013
    ..g. osteogenesis isolated form PDL from the age-match smokers to nonsmokers...
  11. The Roles of FAM20C (DMP4) in Odontogenesis and Osteogenesis
    Chunlin Qin; Fiscal Year: 2013
    ..Our recent studies have established that this novel molecule plays crucial roles in odontogenesis and osteogenesis. We have discovered that the inactivation of Fam20C in mice leads to: 1) significant defects in the dentin, ..
  12. Regulation of Osteoblast Function by Megakaryocytes: Key Signaling Proteins
    Melissa A Kacena; Fiscal Year: 2013
    ..remain to be determined, these observations suggest that the interaction of MKs with OBs results in increased osteogenesis. Furthermore, in OBs co-cultured with MKs, the expression of the cell cycle arrest protein Retinoblastoma (Rb), ..
  13. Correlating and Quantifying Mechanically-induced Bone Cell Microdamage
    Marnie M Saunders; Fiscal Year: 2012
    ..loading is critical to eradicating these conditions, as well as improving fracture healing and distraction osteogenesis outcomes, developing bone substitutes for tissue engineering, and improving implant designs...
  14. Molecular Pathogenesis of Chronic Kidney Disease-Dependent Vascular Calcification
    Makoto Miyazaki; Fiscal Year: 2013
    ..element-binding protein (CREB) family of basic zipper-containing transcription factors that regulates osteogenesis and also mediates unfolded protein response (UPR) in the endoplasmic reticulum (ER)...
  15. Comparison of Bisphosphonate Treatment Regimens on Skeletal Growth &Biomechanics
    Marie Demay; Fiscal Year: 2013
    ..in the pediatric population was initially pioneered for compassionate use in children with severe osteogenesis imperfecta, these medications are being increasingly used for other disorders, ranging in severity from the ..
  16. Synthesis of bone-selective osteogenic oxysterols
    Frank Stappenbeck; Fiscal Year: 2013
    ..Parhami et al. discovered that specific naturally- occurring oxysterols induce osteogenesis when applied to MSCs while inhibiting their adipogenesis...
  17. Assessing the Impact of Osteogenesis Imperfecta on Non-Skeletal Systems
    Laura Tosi; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Osteogenesis imperfecta (OI) is a rare, heterogeneous disease of connective tissue that affects about 30,000 persons in the United States...
  18. The Insulin/IGF-I Axis in Diabetic Osteopathy
    John L Fowlkes; Fiscal Year: 2013
    ..To clarify mechanisms by which insulin and/or IGF-I modulate osteogenesis, and how deficiencies or impaired signaling of each may contribute to diabetic osteopathy, we will examine ..
  19. Connexin 43 in Bone Remodeling
    Roberto Civitelli; Fiscal Year: 2013
    ..experiments will use genetic mouse models wherein Gja1 is selectively ablated or mutated at different stages of osteogenesis. Results will establish the mechanisms by which Cx43 modulates cortical bone and how such mechanisms cause the ..
  20. Role of Angiogenic CXC Chemokines in Intramembranous Bone Repair
    Dean T Yamaguchi; Fiscal Year: 2013
    ..Chemotaxis will be assessed by Transwell assay and osteogenesis determined by mRNA expression of various osteogenic markers...
  21. ADULT STEM CELL SUPPRESSION DUE TO ALCOHOL INDUCED MICROENVIRONMENT ALTERATIONS
    Mandi J Lopez; Fiscal Year: 2010
    ..Adipose tissue-derived stromal cells (ASCs) promote osteogenesis both in vivo and in vitro...
  22. GLYCOSPHINGOLIPID METABOLISM AND MENTAL RETARDATION
    Glyn Dawson; Fiscal Year: 2013
    ..growth and skeletal development through regulation of AktP and the mTor pathway, creating a model for human osteogenesis imperfecta...
  23. Translational Optimization of Bone Regeneration in the Irradiated Mandible
    Steven R Buchman; Fiscal Year: 2013
    ..Distraction Osteogenesis (DO), the creation of new bone by the gradual separation of two osteogenic fronts, generates an anatomical and ..
  24. Role of Angiogenesis in Distraction Osteogenesis
    LOUIS CHARLES GERSTENFELD; Fiscal Year: 2013
    ..A murine model of distraction osteogenesis (DO) will be used to address this hypothesis...
  25. Optimizing Normal Collagen Replacement in Osteogenesis Imperfecta
    Sarah L Dallas; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Osteogenesis imperfecta (OI) is a genetic disorder in which the bones are extremely brittle and highly susceptible to fracture...
  26. High Resolution Peripheral Quantitative CT
    MARY BETH LEONARD; Fiscal Year: 2013
    ..These studies span the spectrum from a clinical trial of mesenchymal stem cell therapy to treat severe osteogenesis imperfecta in children, to a randomized weight loss and exercise intervention trial in a multi-ethnic cohort of ..
  27. Individual Predoctoral Dental Scientist Fellowship
    MELISSA ANNE TALBERT; Fiscal Year: 2011
    ..Although it is well established that metal ions impair osteogenesis, the molecular mechanisms by which metal ions direct tissue differentiation have yet to be identified...
  28. Mechanical Control of Mesenchymal Stem Cell Lineage Allocation
    JANET E RUBIN; Fiscal Year: 2013
    ..selection, as this should identify modifiable steps in pathways that suppress adipogenesis and stimulate osteogenesis. PUBLIC HEALTH RELEVANCE: Evolution has led to interrelationships between bone and fat together to allow ..
  29. Transcriptional Regulation of Indian Hedgehog Expression in Chondrocytes
    Xiangli Yang; Fiscal Year: 2012
    PROJECT SUMMARY/ABSTRACT During endochondral bone formation, osteogenesis and chondrogenesis are linked and highly coordinated. However, the regulatory mechanisms responsible for coordinating osteogenesis and chondrogenesis remain unclear...
  30. The Mechanism of Uremia Induced Vascular Calcification
    Sharon M Moe; Fiscal Year: 2013
    ..applicant): Patients with CKD have increased vascular calcification and the pathogenesis parallels normal bone osteogenesis. We have previously demonstrated that uremia is a major risk factor and in vitro studies show that the effect ..
  31. Studies of Chondroyte Maturation
    Cristina C Teixeira; Fiscal Year: 2010
    ..Chondrocytes also play crucial roles in fracture repair and distraction osteogenesis. Soon, it may be possible to accelerate bone healing by manipulating chondrocyte hypertrophy, death and bone ..
  32. Role of the Leprecan Genes in Skeletal Formation
    Roy Morello; Fiscal Year: 2013
    ..of the CRTAP gene, encoding cartilage associated protein, and the discovery that its mutations cause recessive osteogenesis imperfecta (OI) have led to the identification of additional non-collagen gene mutations responsible for this ..
  33. Structural microenvironment of bone marrow stem cells
    FRANCESCO B RAMIREZ; Fiscal Year: 2013
    ..the following two questions: (a) What are the phenotypic consequences of fibrillin-1 deficiency for BM-based osteogenesis and hematopoiesis?;(b) Does fibrillin-1 coordinate MSC-supported HSC performance? Accordingly, we propose to: (..
  34. Mesenchymal Regulation of Jaw Bone Length
    Erin L Ealba; Fiscal Year: 2013
    ..and targets of the Transforming Growth Factor-Beta (TGF2) since they are known to play critical roles during osteogenesis, and their expression is altered in chimeras...
  35. Mechanotransduction Approach to Improve Bone Quality in Osteogenesis Imperfecta
    Charlotte L Phillips; Fiscal Year: 2012
    ..b>Osteogenesis imperfecta (OI) is a heritable connective tissue disorder characterized by small stature, reduced bone mineral ..
  36. PTHrP/IHH Microenvironment Control for Growth Plate Tissue Engineering
    JUAN MANUEL TABOAS; Fiscal Year: 2013
    ..patients with GP and compromised facial and extremity bone injuries, patients are often subject to distraction osteogenesis, a burdensome and prolonged (3-6 months) procedure using external fixators that pierce the skin, cause great ..
  37. Molecular Control of MSC differentiation and Bone Formation by KDM4B
    Cun Yu Wang; Fiscal Year: 2013
    ..progenitor cells with self-renewal capabilities and multilineage differentiation potentials including osteogenesis, chondrogenesis and adipogenesis...
  38. Directing Stem Cell Differentiation on Biologically Inspired Micropatterned Surfa
    Anita Shukla; Fiscal Year: 2012
    ..multipotent autologous cell source that have shown promise in the treatment of a variety of diseases, including osteogenesis imperfecta and left ventricular dysfunction...
  39. Ceramic scaffolds with engineered topography and chemistry
    ISABELLE L DENRY; Fiscal Year: 2013
    ..step will promote a complex three-dimensional surface micro and nanotopography later stimulating contact osteogenesis. The effect of ion-exchange on surface chemistry, solubility and bioactivity of the scaffolds will be tested in ..
  40. Targeted Correction of Dominant Mutations of Type I Collagen Causing Severe OI
    David W Rowe; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Historically, osteogenesis imperfecta (OI) has been the paradigm for the study of a dominantly inherited disease of connective tissue resulting from a mutation that disrupts the three dimensional ..
  41. Collagen Diversity and Pathobiology in Skeletal Tissues
    David R Eyre; Fiscal Year: 2013
    ..a1(I) and a1(II) chains fails to be hydroxylated in collagens of the crtap mouse and recessive forms of human osteogenesis imperfecta (O.I.) caused by CRTAP or LEPRE1 mutations...
  42. Human Embryonic Stem Cells in Calcium Phosphate Constructs for Bone Regeneration
    HUAKUN XU; Fiscal Year: 2013
    ..If indeed, hESCs delivered via CPC are superior in osteogenesis compared to the gold-standard hBMSCs, which is anticipated to be shown by this project for the first time, the ..
  43. Osteoblast and Odontoblast Specific Regulatory Action of Runx2 for Bone and Tooth
    MITRA D ADHAMI; Fiscal Year: 2013
    ..the osteoblast-commitment phase report osteopenia, suggesting that Runx2 acts as an inhibitor in late stages of osteogenesis. However, it is known that gene expression of Runx2 increases throughout embryonic development, and Runx2 ..
  44. Identification of gene involved in late stage zebrafish craniofacial development
    REBECCA ANN ANDERSON; Fiscal Year: 2013
    ..Defects in the processes of chondrogenesis and/or osteogenesis lead to craniofacial defects and skeletal dysplasias, and many of the genes responsible for these skeletal ..
  45. Injectable Anabolic Biomaterials for Bone Regeneration
    LAKSHMI SREEDHARAN NAIR; Fiscal Year: 2013
    ..to evaluate in vivo the efficacy of the injectable rhLfG in enhancing rBMSC survival, proliferation and osteogenesis, using a rat calvarial bone defect model...
  46. Novel Mechanism of PTH Effects on Bone Metabolism
    Bin Wang; Fiscal Year: 2013
    ..transcription factor 2 (Runx2), and b-catenin, the activities of which can be induced by PTH and involved in osteogenesis, are also down regulated by a ubiquitin-sensitive pathway...
  47. DYNAMICS OF ASSEMBLY OF BONE MATRIX PROTEINS
    Sarah L Dallas; Fiscal Year: 2013
    ..underlying bone formation and have key implications for skeletal diseases such as osteoporosis, arthritis, osteogenesis imperfecta and bone diseases related to ECM proteins...
  48. Targeted Stem Cell Therapy Coupling Angiogenesis and Osteogenesis for Bone Defect
    Selvarangan Ponnazhagan; Fiscal Year: 2013
    ..Thus, new therapeutic approaches coupling angiogenesis to osteogenesis using MSC as genetically-engineered stem cell source will greatly advance patient management and reduce ..
  49. Molecular Signaling in Oxidative Stress-induced Vascular Calcification
    Yabing Chen; Fiscal Year: 2013
    ..Emerging evidence supports the concept that vascular calcification resembles the process of osteogenesis. Increased oxidative stress and production of reactive oxygen species (ROS) accelerate the progression of ..
  50. MAP KINASE REGULATION OF OSTEOBLAST FUNCTION
    RENNY THEODORE FRANCESCHI; Fiscal Year: 2013
    ..Evaluate the role of MAP kinase phosphorylation of RUNX2 and PPAR? in the reciprocal control of osteogenesis and adipogenesis. 2...
  51. MOLECULAR BASIS OF TOOTH ERUPTION
    Shaomian Yao; Fiscal Year: 2012
    ..especially those genes upregulated in the basal one-half of the DF, promote tooth eruption by regulating the osteogenesis needed for bone formation that occurs at the base of the alveolar bony crypt...
  52. The role of CTGF in bone cell development and function
    Steven N Popoff; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): CTGF has recently emerged as an important growth factor in osteogenesis. Previous studies in our laboratory and others have demonstrated that recombinant CTGF promotes proliferation, matrix production ..
  53. GENE TARGETING STRATEGIES FOR THE TREATMENT OF OSTEOGENESIS IMPERFECTA
    David W Russell; Fiscal Year: 2010
    b>Osteogenesis Imperfecta (Ol) is a genetic disease caused by mutations in the type I collagen genes COL1A1 or COL1A2 that can result in major skeletal abnormalities, fractures, and premature death...
  54. Mesenchymal Regulation of Osteogenesis
    Richard A Schneider; Fiscal Year: 2013
    ..and Bone Morphogenetic Protein (BMP) pathways such as Runx2, Dlx5, and Msx1, since they are known to affect osteogenesis and their expression is altered in chimeras...
  55. MSC Therapy & Immune Response in Children with Osteogenesis Imperfecta
    Edwin M Horwitz; Fiscal Year: 2010
    ..infusion of ex vivo- expanded MSCs unequivocally (but transiently) stimulates growth in children with severe osteogenesis imperfecta (OI), a genetic disorder of bone in which the characteristic growth deficiency has no proven ..
  56. Defining the molecular mechanisms underlying human RPE plasticity
    Sally Temple; Fiscal Year: 2013
    ..with RPE metaplasia is ossification, hence it is particularly important to understand how RPE cells undergo osteogenesis. In aim 3 we will focus on the steps RPE move through when transitioning towards this fate, evaluating whether ..
  57. Synergistic effects of conjugated linoleic acid and calcium on bone mass
    Yeonhwa Park; Fiscal Year: 2009
    ..adipogenesis by CLA is a significant but not critical contributing factor for CLA's beneficial effects on osteogenesis. The rationale for this revised application is that investigating the role of CLA specifically on balancing ..
  58. Pro-peptide gene delivery for treating prostate cancer bone metastases
    Marxa L Figueiredo; Fiscal Year: 2013
    ..bone metastases and also contain dual therapeutic domains composed of IL6R and IL11R antagonist peptides and an osteogenesis-promoting peptide, osteostatin...
  59. Evaluation of a Novel Anti-Resorptive Therapy for Osteogenesis Imperfecta
    PATRICIA A COLLIN-OSDOBY; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Although there is presently no cure for Osteogenesis Imperfecta (OI), bisphosphonates are commonly used as anti-resorptive drugs to reduce the severity of OI-related bone loss and maintain vertebral ..
  60. The Role of Osteoactivin in Osteoblast Development and Function
    Fayez F Safadi; Fiscal Year: 2013
    ..Treatment with recombinant OA promotes bone formation in vivo. The importance of OA in osteogenesis was confirmed in mice with the null allele for OA and in mice with a natural mutation in the OA gene caused a ..
  61. Combined Use of BMP-2 and Low Intensity Pulsed Ultrasound in Bone Regeneration
    AMARJIT SINGH VIRDI; Fiscal Year: 2010
    ..research also could benefit patients undergoing surgical procedures such as joint replacement, distraction osteogenesis and dental implant placement in which bone regeneration is integral to successful outcomes...
  62. Directing mesenchymal stem cells to bone to augment bone formation
    Wei Yao; Fiscal Year: 2013
    ..Aging reduces the number of mesenchymal stem cells (MSCs) in the bone marrow which leads to impairment of osteogenesis and subsequent bone loss (osteoporosis)...
  63. NEW GENE THERAPY FOR CONNECTIVE TISSUE DISEASES
    Eric Kmiec; Fiscal Year: 1999
    ..advance in treating severe and nondurable diseases such as hypophosphatasia, chondrodysplasia and perhaps even osteogenesis imperfecta...
  64. CELL AND GENE THERAPY IN A MOUSE MODEL OF HUMAN OSTEOGEN
    Christopher Niyibizi; Fiscal Year: 2000
    b>Osteogenesis imperfecta (OI) is a group of heritable disorders of connective tissue whose common feature is bone fragility...
  65. Development of a biodegradable load-bearing DBM carrier
    TIEN MIN CHU; Fiscal Year: 2006
    ..Current treatment options including autografts, allografts, and distraction osteogenesis have brought forth successes, yet are still with many limitations...
  66. REGULATION OF PROLYL HYDROXYLASE
    Richard Berg; Fiscal Year: 1992
    ..pulmonary fibrosis, cirrhosis, atherosclerosis, acute and chronic inflammation, Ehler's Danlos syndromes, Osteogenesis Imperfecta and wound healing are associated with a change in collagen metabolism...
  67. BONE MARROW TRANSPLANTATION IN OIM--A GENE THERAPY MODEL
    DANIEL MC BRIDE; Fiscal Year: 2001
    ..to form bone throughout an organisms life suggests the continuous presence of a reservoir of cells capable of osteogenesis. Osteoblasts are the differentiated cell type responsible for the biosynthesis, secretion, and orderly ..
  68. Load-Driven Bone Lengthening
    Ping Zhang; Fiscal Year: 2010
    ..Although distraction osteogenesis is effective to treat the patients with limb length discrepancy, this invasive procedure occasionally generates ..
  69. BIOMIMETICS OF BONE REGENERATION
    Jill Helms; Fiscal Year: 2002
    ..We have developed a mouse model of distraction osteogenesis, a clinical procedure which uses a defined mechanical force to induce the formation of bone...
  70. THE EFFECT OF FIBRONECTIN ANTAGONISTS ON OSTEOGENESIS IN
    Amr Moursi; Fiscal Year: 2001
    ..A therapeutic agent which could prevent re-ossification of the excised sutures by transiently blocking osteogenesis would be an effective adjunct to surgery...
  71. Regulated Osteochondrogenesis of Human Mesenchymal Stem Cells Using Gene Delivery
    Antonios Mikos; Fiscal Year: 2009
    ..gene delivery of transcription factors Sox-5, Sox-6, Sox-9 (Sox trio) for chondrogenesis, and Runx-2 for osteogenesis. Each of these plasmids will be complexed with a novel polymeric gene delivery vector developed in our ..
  72. Investigating Ultrastructural Collagen Changes in Osteogenesis Imperfecta
    Joseph Wallace; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): The lack of understanding of how altered collagen structure in Osteogenesis Imperfecta (Ol) translates to insufficiencies in tissue integrity is a critical impediment to the implementation of ..
  73. Targeting bone homing of the MSCs to augment bone formation
    Wei Yao; Fiscal Year: 2010
    ..A decrease in the number of mesenchymal stem cells (MSCs) in bone marrow with aging leads to reduced osteogenesis and thus reduces bone formation and increases bone fragility...
  74. MECHANICAL ENVIRONMENT IN MANDIBULAR DISTRACTION
    Susan Herring; Fiscal Year: 2006
    Distraction osteogenesis (DO) is a means to elongate bone by placing tensile loads on a healing osteotomy...
  75. Treatment of Osteopenia in Children with Cerebral Palsy
    Richard Henderson; Fiscal Year: 2002
    Osteopenia resulting in fractures with minimal trauma is a common problem in many pediatric conditions such as osteogenesis imperfecta, juvenile idiopathic osteoporosis, muscular dystrophy, and myelodysplasia (spina bifida)...
  76. Novel Bone Graft Material With Osteoinductive and Hemostatic Function
    Bo Han; Fiscal Year: 2009
    ..It is non-aqueous and non-inflammatory;it does not increase the risk of infection or inhibit osteogenesis. This combination of properties makes Ostene an ideal carrier for delivery of DBM to any bone defect...
  77. Fibronectin Antagonists in Cranial Suture Formation
    Amr Moursi; Fiscal Year: 2006
    ..A therapeutic agent which could prevent re-ossification of the excised sutures by transiently blocking osteogenesis would be an effective adjunct to surgery...
  78. Osteogenesis: Potentiation With Bone Tropic Peptides
    Gary Balian; Fiscal Year: 2007
    ..library will specifically bind to cells isolated form bone and bone marrow, and have the potential to modulate osteogenesis in vitro and bone regeneration in vivo. This study has 3 aims...