protein transport


Summary: The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.

Top Publications

  1. doi Tetherin inhibits retrovirus release and is antagonized by HIV-1 Vpu
    Stuart J D Neil
    Aaron Diamond AIDS Research Center and Laboratory of Retrovirology, The Rockefeller University, 455 First Avenue, New York, New York 10016, USA
    Nature 451:425-30. 2008
  2. pmc Membrane lipids: where they are and how they behave
    Gerrit van Meer
    Bijvoet Center and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    Nat Rev Mol Cell Biol 9:112-24. 2008
  3. doi Ceramide triggers budding of exosome vesicles into multivesicular endosomes
    Katarina Trajkovic
    Centre for Biochemistry and Molecular Cell Biology, University of Gottingen, 37073 Gottingen, Germany
    Science 319:1244-7. 2008
  4. ncbi Development by self-digestion: molecular mechanisms and biological functions of autophagy
    Beth Levine
    Department of Medicine, Columbia University, New York, NY 10032, USA
    Dev Cell 6:463-77. 2004
  5. ncbi Local, efflux-dependent auxin gradients as a common module for plant organ formation
    Eva Benkova
    Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, 72076 Tubingen, Germany
    Cell 115:591-602. 2003
  6. pmc Parkin is recruited selectively to impaired mitochondria and promotes their autophagy
    Derek Narendra
    Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 183:795-803. 2008
  7. ncbi Drosophila pink1 is required for mitochondrial function and interacts genetically with parkin
    Ira E Clark
    Nature 441:1162-6. 2006
  8. ncbi The Wnt signaling pathway in development and disease
    Catriona Y Logan
    Department of Developmental Biology, Beckman Center, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
    Annu Rev Cell Dev Biol 20:781-810. 2004
  9. doi Exosomes--vesicular carriers for intercellular communication
    Mikael Simons
    Max Planck Institute for Experimental Medicine, Gottingen, Germany
    Curr Opin Cell Biol 21:575-81. 2009
  10. pmc One step at a time: endoplasmic reticulum-associated degradation
    Shruthi S Vembar
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Nat Rev Mol Cell Biol 9:944-57. 2008

Detail Information

Publications390 found, 100 shown here

  1. doi Tetherin inhibits retrovirus release and is antagonized by HIV-1 Vpu
    Stuart J D Neil
    Aaron Diamond AIDS Research Center and Laboratory of Retrovirology, The Rockefeller University, 455 First Avenue, New York, New York 10016, USA
    Nature 451:425-30. 2008
    ..Vpu co-localized with CD317 and inhibited these effects. Inhibition of Vpu function and consequent mobilization of tetherin's antiviral activity is a potential therapeutic strategy in HIV/AIDS...
  2. pmc Membrane lipids: where they are and how they behave
    Gerrit van Meer
    Bijvoet Center and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    Nat Rev Mol Cell Biol 9:112-24. 2008
    ..enzymes, translocases and transporters, plus the intrinsic physical phase behaviour of lipids and their interactions with membrane proteins, to create the unique compositions and multiple functionalities of their individual membranes?..
  3. doi Ceramide triggers budding of exosome vesicles into multivesicular endosomes
    Katarina Trajkovic
    Centre for Biochemistry and Molecular Cell Biology, University of Gottingen, 37073 Gottingen, Germany
    Science 319:1244-7. 2008
    ..Purified exosomes were enriched in ceramide, and the release of exosomes was reduced after the inhibition of neutral sphingomyelinases. These results establish a pathway in intraendosomal membrane transport and exosome formation...
  4. ncbi Development by self-digestion: molecular mechanisms and biological functions of autophagy
    Beth Levine
    Department of Medicine, Columbia University, New York, NY 10032, USA
    Dev Cell 6:463-77. 2004
    ..In this review, we summarize our current knowledge about the molecular machinery of autophagy and the role of the autophagic machinery in eukaryotic development...
  5. ncbi Local, efflux-dependent auxin gradients as a common module for plant organ formation
    Eva Benkova
    Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, 72076 Tubingen, Germany
    Cell 115:591-602. 2003
    ..Our results suggest that PIN-dependent, local auxin gradients represent a common module for formation of all plant organs, regardless of their mature morphology or developmental origin...
  6. pmc Parkin is recruited selectively to impaired mitochondria and promotes their autophagy
    Derek Narendra
    Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 183:795-803. 2008
    ..These results show that Parkin promotes autophagy of damaged mitochondria and implicate a failure to eliminate dysfunctional mitochondria in the pathogenesis of Parkinson's disease...
  7. ncbi Drosophila pink1 is required for mitochondrial function and interacts genetically with parkin
    Ira E Clark
    Nature 441:1162-6. 2006
    ..The role of the pink1-parkin pathway in regulating mitochondrial function underscores the importance of mitochondrial dysfunction as a central mechanism of Parkinson's disease pathogenesis...
  8. ncbi The Wnt signaling pathway in development and disease
    Catriona Y Logan
    Department of Developmental Biology, Beckman Center, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
    Annu Rev Cell Dev Biol 20:781-810. 2004
    ..The next few years are likely to see novel therapeutic reagents aimed at controlling Wnt signaling in order to alleviate these conditions...
  9. doi Exosomes--vesicular carriers for intercellular communication
    Mikael Simons
    Max Planck Institute for Experimental Medicine, Gottingen, Germany
    Curr Opin Cell Biol 21:575-81. 2009
    ..Only recently light have been shed on the subcellular compartments and mechanisms involved in their biogenesis and secretion opening new avenues to understand their functions...
  10. pmc One step at a time: endoplasmic reticulum-associated degradation
    Shruthi S Vembar
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Nat Rev Mol Cell Biol 9:944-57. 2008
    ..Here, we summarize our current understanding of each step during ERAD, with emphasis on the factors that catalyse distinct activities...
  11. doi A role for ubiquitin in selective autophagy
    Vladimir Kirkin
    Institute of Biochemistry II and Cluster of Excellence Macromolecular Complexes, Goethe University, Theodor Stern Kai 7, Frankfurt, Germany
    Mol Cell 34:259-69. 2009
    ..This review explores the hypothesis that ubiquitin represents a selective degradation signal suitable for targeting various types of cargo, ranging from protein aggregates to membrane-bound organelles and microbes...
  12. pmc Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum
    Elizabeth L Axe
    Signalling Programme, Babraham Institute, Babraham, Cambridge, England, UK
    J Cell Biol 182:685-701. 2008
    ..This PI(3)P-enriched compartment may be involved in autophagosome biogenesis. Its dynamic relationship with the ER is consistent with the idea that the ER may provide important components for autophagosome formation...
  13. ncbi The deacetylase HDAC6 regulates aggresome formation and cell viability in response to misfolded protein stress
    Yoshiharu Kawaguchi
    Department of Pharmacology and Cancer Biology, Duke University, Durham, NC 27710, USA
    Cell 115:727-38. 2003
    ..These findings identify HDAC6 as a crucial player in the cellular management of misfolded protein-induced stress...
  14. doi The ESCRT machinery in endosomal sorting of ubiquitylated membrane proteins
    Camilla Raiborg
    Centre for Cancer Biomedicine, Norwegian Radium Hospital, University of Oslo, Oslo, Norway
    Nature 458:445-52. 2009
    ..The involvement of the ESCRT machinery in suppressing diseases such as cancer, neurodegeneration and infections underscores its importance to the cell...
  15. pmc Drosophila parkin requires PINK1 for mitochondrial translocation and ubiquitinates mitofusin
    Elena Ziviani
    Department of Biomedical Sciences, Medical Research Council Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield S10 2TN, United Kingdom
    Proc Natl Acad Sci U S A 107:5018-23. 2010
    ..We hypothesize that Mfn ubiquitination may provide a mechanism by which terminally damaged mitochondria are labeled and sequestered for degradation by autophagy...
  16. pmc Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids
    Yasemin Sancak
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Cell 141:290-303. 2010
    ..Thus, Rag-Ragulator-mediated translocation of mTORC1 to lysosomal membranes is the key event in amino acid signaling to mTORC1...
  17. pmc Importing mitochondrial proteins: machineries and mechanisms
    Agnieszka Chacinska
    Institut fur Biochemie und Molekularbiologie, ZBMZ, Universitat Freiburg, 79104 Freiburg, Germany
    Cell 138:628-44. 2009
    ..Here, we discuss recent insights into the importing and sorting of mitochondrial proteins and their contributions to mitochondrial biogenesis...
  18. ncbi A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis
    Maxence V Nachury
    Genentech, Inc, South San Francisco, CA 94080, USA
    Cell 129:1201-13. 2007
    ..Conversely, preventing Rab8(GTP) production blocks ciliation in cells and yields characteristic BBS phenotypes in zebrafish. Our data reveal that BBS may be caused by defects in vesicular transport to the cilium...
  19. doi Ciliogenesis: building the cell's antenna
    Hiroaki Ishikawa
    Department of Biochemistry and Biophysics, University of California, San Francisco, BOX 2200 GH N372F, 600 16th Street, San Francisco, California 94158, USA
    Nat Rev Mol Cell Biol 12:222-34. 2011
    ..As cilia are important in sensing and processing developmental signals and directing the flow of fluids such as mucus, defects in ciliogenesis and length control are likely to underlie a range of cilium-related human diseases...
  20. pmc Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system
    Stefan Pukatzki
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:1528-33. 2006
    ..Thus, the genes encoding the VAS-related, type VI secretion system likely play an important conserved function in microbial pathogenesis and represent an additional class of targets for vaccine and antimicrobial drug-based therapies...
  21. pmc The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia
    Hua Jin
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305 5345, USA
    Cell 141:1208-19. 2010
    ..Thus, we propose that trafficking of BBSome cargoes to cilia entails the coupling of BBSome coat polymerization to the recognition of sorting signals by the BBSome...
  22. doi Nonproteolytic functions of ubiquitin in cell signaling
    Zhijian J Chen
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
    Mol Cell 33:275-86. 2009
    ..Recent advances in understanding ubiquitination in protein kinase activation and DNA repair are discussed to illustrate the nonproteolytic functions of ubiquitin in cell signaling...
  23. ncbi The AAA ATPase Cdc48/p97 and its partners transport proteins from the ER into the cytosol
    Y Ye
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 414:652-6. 2001
    ..We propose that the Cdc48/p97-Ufd1-Npl4 complex extracts proteins from the ER membrane for cytosolic degradation...
  24. pmc Amyloid precursor protein trafficking, processing, and function
    Gopal Thinakaran
    Department of Neurobiology, The University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 283:29615-9. 2008
    ..This minireview summarizes salient features of APP trafficking and amyloidogenic processing and discusses the putative biological functions of APP...
  25. ncbi Impaired degradation of mutant alpha-synuclein by chaperone-mediated autophagy
    Ana Maria Cuervo
    Department of Anatomy and Structural Biology, Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Science 305:1292-5. 2004
    ..These findings may underlie the toxic gain-of-function by the mutants...
  26. ncbi Global analysis of protein localization in budding yeast
    Won Ki Huh
    Howard Hughes Medical Institute, University of California San Francisco, Department of Biochemistry and Biophysics, 600 16th Street, San Francisco, California 94143 2240, USA
    Nature 425:686-91. 2003
  27. pmc GLUT4 exocytosis
    Jacqueline Stöckli
    Diabetes and Obesity Program, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia
    J Cell Sci 124:4147-59. 2011
    ..This regulatory process clearly involves the Ser/Thr kinase AKT and the exquisite manner in which this single metabolic process is regulated makes it a likely target for lesions that might contribute to metabolic disease...
  28. pmc Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins
    Eisuke Itakura
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Bunkyo ku, Tokyo, Japan
    Autophagy 6:764-76. 2010
    ..These hierarchical analyses suggest that ULK1, Atg14 and VMP1 localize to the ER-associated autophagosome formation sites in a PI3-kinase activity-independent manner...
  29. doi Integrins: masters and slaves of endocytic transport
    Patrick T Caswell
    Beatson Institute for Cancer Research, Cancer Research UK, Glasgow, G61 3BX, Scotland
    Nat Rev Mol Cell Biol 10:843-53. 2009
    ..Furthermore, integrins can exert control over the trafficking of other receptors in a way that drives cancer cell invasion and tumour angiogenesis...
  30. ncbi Protein translocation across the bacterial cytoplasmic membrane
    Arnold J M Driessen
    Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute for Advanced Materials, University of Groningen, Haren, The Netherlands
    Annu Rev Biochem 77:643-67. 2008
    ..This review summarizes the present knowledge of the mechanism and structure of the Sec translocase, with a special emphasis on unresolved questions and topics of current research...
  31. pmc The clathrin adaptor AP-1A mediates basolateral polarity
    Diego Gravotta
    Margaret Dyson Vision Research Institute, Department of Ophthalmology, Weill Cornell Medical College, New York, NY 10065, USA
    Dev Cell 22:811-23. 2012
    ..The basolateral sorting function of AP-1A reported here establishes AP-1 as a major regulator of epithelial polarity...
  32. pmc SNX27 mediates retromer tubule entry and endosome-to-plasma membrane trafficking of signalling receptors
    Paul Temkin
    Department of Psychiatry, University of California at San Francisco, San Francisco, California 94158, USA
    Nat Cell Biol 13:715-21. 2011
    ..The present results identify a role for retromer in endocytic trafficking of signalling receptors, in regulating a receptor-linked signalling pathway, and in mediating direct endosome-to-plasma membrane traffic...
  33. pmc Synaptic AMPA receptor plasticity and behavior
    Helmut W Kessels
    Department of Neuroscience, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    Neuron 61:340-50. 2009
    ..We argue that monitoring and manipulating synaptic AMPAR trafficking represents an attractive means to study cognitive function and dysfunction in animal models...
  34. pmc PINK1-dependent recruitment of Parkin to mitochondria in mitophagy
    Cristofol Vives-Bauza
    Department of Neurology, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 107:378-83. 2010
    ..Thus by impairing this process, mutations in either Parkin or PINK1 may alter mitochondrial turnover which, in turn, may cause the accumulation of defective mitochondria and, ultimately, neurodegeneration in Parkinson's disease...
  35. doi Mitochondrial protein import: from proteomics to functional mechanisms
    Oliver Schmidt
    Institut fur Biochemie und Molekularbiologie, ZBMZ, Universitat Freiburg, Stefan Meier Strabetae 17, 79104 Freiburg, Germany
    Nat Rev Mol Cell Biol 11:655-67. 2010
    ..Protein translocases do not function as independent units but are integrated into dynamic networks and are connected to machineries that function in bioenergetics, mitochondrial morphology and coupling to the endoplasmic reticulum...
  36. ncbi A translocation signal for delivery of oomycete effector proteins into host plant cells
    Stephen C Whisson
    Plant Pathology Programme, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK
    Nature 450:115-8. 2007
    ..infestans genome. Identification of this class of proteins provides unparalleled opportunities to determine how oomycetes manipulate hosts to establish infection...
  37. pmc Coordinated protein sorting, targeting and distribution in polarized cells
    Ira Mellman
    Genentech, Inc, 1 DNA Way, South San Francisco, California 94080, USA
    Nat Rev Mol Cell Biol 9:833-45. 2008
    ..The integration of these mechanisms into a complex and dynamic network is crucial for normal tissue function and is often defective in disease states...
  38. pmc GTPase networks in membrane traffic
    Emi Mizuno-Yamasaki
    Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371 8512, Japan
    Annu Rev Biochem 81:637-59. 2012
    ..As each GTPase recruits a unique set of effectors, these transitions help to define changes in the functionality of the membrane compartments with which they are associated...
  39. pmc Vinculin controls focal adhesion formation by direct interactions with talin and actin
    Jonathan D Humphries
    Wellcome Trust Centre for Cell Matrix Research, University of Manchester, Manchester M13 9PT, England, UK
    J Cell Biol 179:1043-57. 2007
    ..The vinculin tail, however, is responsible for the functional link of FAs to the actin cytoskeleton. We propose a new model that explains how vinculin orchestrates FAs...
  40. doi Prions hijack tunnelling nanotubes for intercellular spread
    Karine Gousset
    Unité de Trafic Membranaire et Pathogénèse, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    Nat Cell Biol 11:328-36. 2009
    ..We therefore propose that TNTs are involved in the spreading of PrP(Sc) within neurons in the CNS and from the peripheral site of entry to the PNS by neuroimmune interactions with dendritic cells...
  41. pmc The Atg16L complex specifies the site of LC3 lipidation for membrane biogenesis in autophagy
    Naonobu Fujita
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 19:2092-100. 2008
    ..We propose that the Atg16L complex is a new type of E3-like enzyme that functions as a scaffold for LC3 lipidation by dynamically localizing to the putative source membranes for autophagosome formation...
  42. pmc Colicin biology
    Eric Cascales
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Microbiol Mol Biol Rev 71:158-229. 2007
    ..The mechanisms of inhibition by specific and cognate immunity proteins are presented. Finally, the use of colicins as laboratory or biotechnological tools and their mode of evolution are discussed...
  43. pmc Road to ruin: targeting proteins for degradation in the endoplasmic reticulum
    Melanie H Smith
    Department of Cellular and Molecular Pharmacology, Graduate Group in Biophysics and Howard Hughes Medical Institute, University of California San Francisco, CA 94158, USA
    Science 334:1086-90. 2011
    ..Much less is known about the features used to identify terminally misfolded conformations and the broader role of these pathways in regulating protein half-lives...
  44. doi Molecular motors in neurons: transport mechanisms and roles in brain function, development, and disease
    Nobutaka Hirokawa
    Departments of Cell Biology and Anatomy and Molecular Structure and Dynamics, Graduate School of Medicine, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Neuron 68:610-38. 2010
    ..Finally, it is also evident that molecular motors are critically involved in neuronal disease pathogenesis. Thus, molecular motor research is becoming an exciting frontier of neuroscience...
  45. pmc Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy
    Nao Hosokawa
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Mol Biol Cell 20:1981-91. 2009
    ..ULK1 is dephosphorylated by rapamycin treatment or starvation. These data suggest that mTORC1 suppresses autophagy through direct regulation of the approximately 3-MDa ULK1-Atg13-FIP200 complex...
  46. ncbi Endocytosis and intracellular trafficking of ErbBs
    Alexander Sorkin
    Department of Pharmacology, University of Colorado Denver Anschutz Medical Center, Aurora, Colorado 80045 0508, USA
    Exp Cell Res 315:683-96. 2009
    ..However, these ErbBs are internalized and targeted to lysosomes less efficiently than EGFR. When overexpressed endocytosis-impaired ErbBs may inhibit the internalization and degradation of EGFR...
  47. doi Cytokinin modulates endocytic trafficking of PIN1 auxin efflux carrier to control plant organogenesis
    Peter Marhavy
    Department of Plant Systems Biology, VIB, Technologiepark 927, B 9052 Gent, Belgium
    Dev Cell 21:796-804. 2011
  48. doi The transcellular spread of cytosolic amyloids, prions, and prionoids
    Adriano Aguzzi
    Institute of Neuropathology, University Hospital of Zurich, Schmelzbergstrasse 12, CH 8091 Zurich, Switzerland
    Neuron 64:783-90. 2009
  49. doi Adapting proteostasis for disease intervention
    William E Balch
    Department of Cell Biology and Institute for Childhood and Neglected Diseases, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 319:916-9. 2008
  50. ncbi Exosomes: a common pathway for a specialized function
    Guillaume van Niel
    Institut Curie, CNRS UMR144, 75248 Paris Cedex, France
    J Biochem 140:13-21. 2006
    ..These findings open up new therapeutic and diagnostic strategies...
  51. doi Mechanisms of regulated unconventional protein secretion
    Walter Nickel
    Heidelberg University Biochemistry Center, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Nat Rev Mol Cell Biol 10:148-55. 2009
  52. pmc Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources?
    Frederic Boyer
    CEA, iRTSV, Laboratoire Biologie, Informatique et Mathématiques, Grenoble, France
    BMC Genomics 10:104. 2009
    ..By combining comparative and phylogenetic approaches using more than 500 prokaryotic genomes, we characterized the global T6SS genetic structure in terms of conservation, evolution and genomic organization...
  53. ncbi The mouse Spo11 gene is required for meiotic chromosome synapsis
    P J Romanienko
    Genetics and Biochemistry Branch National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health 20892, Bethesda, MD, USA
    Mol Cell 6:975-87. 2000
    ..We speculate that there is an additional role for Spo11, after it generates DSBs, in synapsis...
  54. doi Toxoplasma sortilin-like receptor regulates protein transport and is essential for apical secretory organelle biogenesis and host infection
    Pierre Julien Sloves
    Center for Infection and Immunity of Lille, Universite Lille Nord de France, Lille, France
    Cell Host Microbe 11:515-27. 2012
    ..the cytoplasmic tail of TgSORTLR recruits cytosolic sorting machinery involved in anterograde and retrograde protein transport. Ectopic expression of the N-terminal TgSORTLR lumenal domain results in dominant negative effects with the ..
  55. doi The ubiquitin-selective segregase VCP/p97 orchestrates the response to DNA double-strand breaks
    Mayura Meerang
    Institute of Pharmacology and Toxicology, University of Zurich Vetsuisse, Winterthurerstrasse 260, 8057 Zurich, Switzerland
    Nat Cell Biol 13:1376-82. 2011
    ..These findings identify the p97-UFD1-NPL4 complex as an essential factor in ubiquitin-governed DNA-damage response, highlighting its importance in guarding genome stability...
  56. pmc Ubiquitin-dependent regulation of COPII coat size and function
    Lingyan Jin
    Department of Molecular and Cell Biology, University of California at Berkeley, California 94720, USA
    Nature 482:495-500. 2012
    ..As a result, ubiquitylation by CUL3-KLHL12 is essential for collagen export, yet less important for the transport of small cargo. We conclude that monoubiquitylation controls the size and function of a vesicle coat...
  57. pmc Non-classical protein secretion in bacteria
    Jannick D Bendtsen
    Center for Biological Sequence Analysis, BioCentrum DTU, Building 208, Technical University of Denmark, DK 2800 Lyngby, Denmark
    BMC Microbiol 5:58. 2005
    ..Some of these proteins also have known roles in the cytoplasm, which means they could be so-called "moon-lightning" proteins having more than one function...
  58. ncbi Regulation of signaling protein function and trafficking by the hsp90/hsp70-based chaperone machinery
    William B Pratt
    Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0632, USA
    Exp Biol Med (Maywood) 228:111-33. 2003
    ..This purified system should facilitate understanding of how eukaryotic hsp70 and hsp90 work together as essential components of a process that alters the conformations of substrate proteins to states that respond in signal transduction...
  59. pmc Strange as it may seem: the many links between Wnt signaling, planar cell polarity, and cilia
    John B Wallingford
    Howard Hughes Medical Institute, Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA
    Genes Dev 25:201-13. 2011
  60. ncbi The genome of the African trypanosome Trypanosoma brucei
    Matthew Berriman
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
    Science 309:416-22. 2005
    ..brucei and the greatest in L. major. Horizontal transfer of genes of bacterial origin has contributed to some of the metabolic differences in these parasites, and a number of novel potential drug targets have been identified...
  61. ncbi Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis
    Jiri Friml
    Max Delbruck Laboratorium in der Max Planck Gesellschaft, 50829 Köln, Germany
    Nature 415:806-9. 2002
    ..In addition, actin-dependent relocalization of PIN3 in response to gravity provides a mechanism for redirecting auxin flux to trigger asymmetric growth...
  62. doi Planar polarized actomyosin contractile flows control epithelial junction remodelling
    Matteo Rauzi
    IBDML, UMR6216 CNRS Université de la Méditerranée, Campus de Luminy, Case 907, 13288 Marseille Cedex 09, France
    Nature 468:1110-4. 2010
    ..Thus, epithelial morphogenesis is not properly reflected by Myosin II steady state distribution but by polarized contractile actomyosin flows that emerge from interactions between E-cadherin and actomyosin networks...
  63. pmc Nuclear trafficking of the epidermal growth factor receptor family membrane proteins
    Y N Wang
    Department of Molecular and Cellular Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Oncogene 29:3997-4006. 2010
    ..Understanding the trafficking mechanisms as to how RTKs are transported from the cell surface to the nucleus will significantly contribute to understanding the functions of the nuclear RTKs...
  64. pmc Arc/Arg3.1 regulates an endosomal pathway essential for activity-dependent β-amyloid generation
    Jing Wu
    Solomon H Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell 147:615-28. 2011
    ..Genetic deletion of Arc reduces Aβ load in a transgenic mouse model of AD. In concert with the finding that patients with AD can express anomalously high levels of Arc, we hypothesize that Arc participates in the pathogenesis of AD...
  65. ncbi Intracellular functions of N-linked glycans
    A Helenius
    Institute of Biochemistry, , , , Switzerland
    Science 291:2364-9. 2001
    ..The division of synthesis and processing between the ER and the Golgi complex represents an evolutionary adaptation that allows efficient exploitation of the potential of oligosaccharides...
  66. pmc Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein
    Olav M Andersen
    Max Delbrueck Center for Molecular Medicine, D 13125 Berlin, Germany
    Proc Natl Acad Sci U S A 102:13461-6. 2005
    ..Consequently, reduced receptor expression in the human brain may increase Abeta production and plaque formation and promote spontaneous AD...
  67. ncbi Exosomes: from biogenesis and secretion to biological function
    Sascha Keller
    German Cancer Research Center DKFZ, Tumor Immunology Program, D010 TP3, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Immunol Lett 107:102-8. 2006
  68. ncbi A bacterial virulence protein suppresses host innate immunity to cause plant disease
    Kinya Nomura
    Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA
    Science 313:220-3. 2006
    ..HopM1 mediates the destruction of AtMIN7 via the host proteasome. Our results illustrate a strategy by which a bacterial pathogen exploits the host proteasome to subvert host immunity and causes infection in plants...
  69. ncbi Rab25 associates with alpha5beta1 integrin to promote invasive migration in 3D microenvironments
    Patrick T Caswell
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
    Dev Cell 13:496-510. 2007
    ..These data indicate that Rab25 contributes to tumor progression by directing the localization of integrin-recycling vesicles and thereby enhancing the ability of tumor cells to invade the extracellular matrix...
  70. doi Cullin3-based polyubiquitination and p62-dependent aggregation of caspase-8 mediate extrinsic apoptosis signaling
    Zhaoyu Jin
    Department of Molecular Oncology, Genentech, Inc, 1 DNA Way, South San Francisco, CA 94080, USA
    Cell 137:721-35. 2009
    ..These results identify a mechanism that positively controls apoptosis signaling by polyubiquitination and aggregation of a key initiator caspase...
  71. pmc Cryo-EM structure of the ribosome-SecYE complex in the membrane environment
    Jens Frauenfeld
    Gene Center, Department for Biochemistry, University of Munich, Munich, Germany
    Nat Struct Mol Biol 18:614-21. 2011
    ..On the basis of our map and molecular dynamics simulations, we present a model of a signal anchor-gated PCC in the membrane...
  72. ncbi Localization of organellar proteins in Plasmodium falciparum using a novel set of transfection vectors and a new immunofluorescence fixation method
    Christopher J Tonkin
    Plant Cell Biology Research Centre, School of Botany, The University of Melbourne, Parkville, VIC 3010, Australia
    Mol Biochem Parasitol 137:13-21. 2004
    ..falciparum proteins...
  73. pmc The type VI secretion toolkit
    Eric Cascales
    Laboratoire d Ingenierie des Systemes Macromoleculaires, IBSM, Centre National de la Recherche Scientifique, Marseille, France
    EMBO Rep 9:735-41. 2008
    ..This review provides an overview of our current knowledge about type VI secretion, focusing on gene regulation, components of the secretion machine, substrate secretion and the cellular consequences for the host cell...
  74. pmc Cleaning up: ER-associated degradation to the rescue
    Jeffrey L Brodsky
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Cell 151:1163-7. 2012
    ..This process is termed endoplasmic-reticulum (ER)-associated degradation (ERAD)...
  75. pmc Ankyrin repeat proteins comprise a diverse family of bacterial type IV effectors
    Xiaoxiao Pan
    Section of Microbial Pathogenesis, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    Science 320:1651-4. 2008
    ..pneumophila-containing vacuole with late endosomes after infection of macrophages, which demonstrates that Ank proteins have effector functions important for bacterial infection of eukaryotic host cells...
  76. pmc Molecular mechanism of multivesicular body biogenesis by ESCRT complexes
    Thomas Wollert
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services, Bethesda, Maryland 20892, USA
    Nature 464:864-9. 2010
    ..The observations explain how the ESCRTs direct membrane budding and scission from the cytoplasmic side of the bud without being consumed in the reaction...
  77. doi Protein sorting receptors in the early secretory pathway
    Julia Dancourt
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Annu Rev Biochem 79:777-802. 2010
    ..Our current understanding of the molecular mechanisms underlying cargo receptor function are described...
  78. ncbi A membrane protein complex mediates retro-translocation from the ER lumen into the cytosol
    Yihong Ye
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 429:841-7. 2004
    ..Derlin-1 interacts with US11, a virally encoded ER protein that specifically targets MHC class I heavy chains for export from the ER, as well as with VIMP, a novel membrane protein that recruits the p97 ATPase and its cofactor...
  79. doi Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation
    Hannah E J Polson
    Secretory Pathways Laboratory, London Research Institute, Cancer Research UK, London, UK
    Autophagy 6:506-22. 2010
    ..In view of our data we propose a role for WIPI2 in the progression of omegasomes into autophagosomes...
  80. ncbi A photoactivatable GFP for selective photolabeling of proteins and cells
    George H Patterson
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 297:1873-7. 2002
    ..Here, we use the photoactivatable GFP both as a free protein to measure protein diffusion across the nuclear envelope and as a chimera with a lysosomal membrane protein to demonstrate rapid interlysosomal membrane exchange...
  81. ncbi A transcriptionally [correction of transcriptively] active complex of APP with Fe65 and histone acetyltransferase Tip60
    X Cao
    The Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9111 USA
    Science 293:115-20. 2001
    ..This complex potently stimulates transcription via heterologous Gal4- or LexA-DNA binding domains, suggesting that release of the cytoplasmic tail of APP by gamma-cleavage may function in gene expression...
  82. pmc Comprehensive identification of protein substrates of the Dot/Icm type IV transporter of Legionella pneumophila
    Wenhan Zhu
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana, United States of America
    PLoS ONE 6:e17638. 2011
    ..Thus, our efforts have identified a large number of novel substrates of the Dot/Icm system and have revealed the diverse features recognizable by this protein transporter...
  83. pmc Identification of a tumour suppressor network opposing nuclear Akt function
    Lloyd C Trotman
    Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, Sloan Kettering Institute, 1275 York Avenue, New York, New York 10021, USA
    Nature 441:523-7. 2006
    ..Our results demonstrate that Pml orchestrates a nuclear tumour suppressor network for inactivation of nuclear pAkt, and thus highlight the importance of AKT compartmentalization in human cancer pathogenesis and treatment...
  84. pmc Generation of cell polarity in plants links endocytosis, auxin distribution and cell fate decisions
    Pankaj Dhonukshe
    Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
    Nature 456:962-6. 2008
    ..It also highlights the link between endocytosis-dependent polarity of individual cells and auxin distribution-dependent cell fate establishment for multicellular patterning...
  85. pmc The mitochondrial p53 pathway
    Angelina V Vaseva
    Department of Pathology, Stony Brook University, Stony Brook, NY 11794, USA
    Biochim Biophys Acta 1787:414-20. 2009
    ..Our review will discuss these studies, focusing on recent advances in the field...
  86. pmc Rab-coupling protein coordinates recycling of alpha5beta1 integrin and EGFR1 to promote cell migration in 3D microenvironments
    Patrick T Caswell
    Beatson Institute for Cancer Research, Bearsden, Glasgow G61 1BD, Scotland, UK
    J Cell Biol 183:143-55. 2008
    ..We conclude that RCP provides a scaffold that promotes the physical association and coordinate trafficking of alpha5beta1 and EGFR1 and that this drives migration of tumor cells into three-dimensional matrices...
  87. pmc Analysis of nucleolar protein dynamics reveals the nuclear degradation of ribosomal proteins
    Yun Wah Lam
    Division of Gene Regulation and Expression, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Curr Biol 17:749-60. 2007
  88. ncbi Phosphorylation of Bax Ser184 by Akt regulates its activity and apoptosis in neutrophils
    Shyra J Gardai
    Program in Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206, USA
    J Biol Chem 279:21085-95. 2004
  89. pmc Genome-scale identification of Legionella pneumophila effectors using a machine learning approach
    David Burstein
    Department of Cell Research and Immunology, George S Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel
    PLoS Pathog 5:e1000508. 2009
    ..pneumophila effectors, predicted over a hundred additional highly probable effectors, and shown the applicability of machine learning algorithms for the identification and characterization of bacterial pathogenesis determinants...
  90. doi Spatial control of EGF receptor activation by reversible dimerization on living cells
    Inhee Chung
    Genentech, Inc, 1 DNA Way, South San Francisco, California 94080, USA
    Nature 464:783-7. 2010
    ..Interestingly, dimers were enriched in the cell periphery in an actin- and receptor-expression-dependent fashion, resulting in a peripheral enhancement of EGF-induced signalling that may enable polarized responses to growth factors...
  91. ncbi Stats: transcriptional control and biological impact
    David E Levy
    Department of Pathology, New York University School of Medicine, New York, New York 10016, USA
    Nat Rev Mol Cell Biol 3:651-62. 2002
    ..In addition to several roles in normal cell decisions, dysregulation of STAT function contributes to human disease, making the study of these proteins an important topic of current research...
  92. pmc Trafficking to the ciliary membrane: how to get across the periciliary diffusion barrier?
    Maxence V Nachury
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305 5345, USA
    Annu Rev Cell Dev Biol 26:59-87. 2010
    ..Finally, some proteins need to exit from cilia, and ubiquitination may regulate this step. The stage is now set to dissect the interplay between signaling and regulated trafficking to and from cilia...
  93. ncbi PML regulates p53 stability by sequestering Mdm2 to the nucleolus
    Rosa Bernardi
    Cancer Biology and Genetics Program, Department of Pathology and Medicine, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Nat Cell Biol 6:665-72. 2004
    ..These findings demonstrate an unexpected role of PML in the nucleolar network for tumour suppression...
  94. doi Membrane protein architects: the role of the BAM complex in outer membrane protein assembly
    Timothy J Knowles
    Cancer Research UK Institute for Cancer Studies, School of Cancer Sciences, Edgbaston, Birmingham, UK
    Nat Rev Microbiol 7:206-14. 2009
    ..Here, we discuss how these structures illuminate the mechanisms responsible for the biogenesis of outer membrane proteins...
  95. ncbi Activity-dependent regulation of dendritic synthesis and trafficking of AMPA receptors
    William Ju
    Nancy Friend Pritzker Laboratory, Department of Psychiatry and Behavioral Sciences, 1201 Welch Road, Stanford University School of Medicine, Palo Alto, California 94304, USA
    Nat Neurosci 7:244-53. 2004
  96. pmc A mechanism for vertebrate Hedgehog signaling: recruitment to cilia and dissociation of SuFu-Gli protein complexes
    Hanna Tukachinsky
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 191:415-28. 2010
    ..Our results support a simple mechanism in which Hh signals at vertebrate cilia cause dissociation of inactive SuFu-Gli complexes, a process inhibited by PKA...
  97. pmc A FAM21-containing WASH complex regulates retromer-dependent sorting
    Timothy S Gomez
    Department of Immunology, Division of Oncology Research and Schulze Center for Novel Therapeutics, College of Medicine, Mayo Clinic, Rochester, MN 55905, USA
    Dev Cell 17:699-711. 2009
  98. pmc Routes, destinations and delays: recent advances in AMPA receptor trafficking
    Jeremy M Henley
    MRC Centre for Synaptic Plasticity, School of Biochemistry, University of Bristol, University Walk, Bristol, UK, BS8 1TD
    Trends Neurosci 34:258-68. 2011
    ..Here, we review recent findings that have provided new insights into AMPAR trafficking and that might lead to the development of novel therapeutic strategies...
  99. pmc Antagonism of tetherin restriction of HIV-1 release by Vpu involves binding and sequestration of the restriction factor in a perinuclear compartment
    Mathieu Dubé
    Laboratory of Human Retrovirology, Institut de recherches cliniques de Montreal IRCM, Montreal, Quebec, Canada
    PLoS Pathog 6:e1000856. 2010
  100. pmc ADP ribosylation factor 6 (ARF6) controls amyloid precursor protein (APP) processing by mediating the endosomal sorting of BACE1
    Ragna Sannerud
    Laboratory of Membrane Trafficking, Center for Human Genetics, Catholic University of Leuven, Leuven, Belgium
    Proc Natl Acad Sci U S A 108:E559-68. 2011
    ..It thereby provides a novel avenue to interfere with Aβ production through a selective modulation of the distinct endosomal transport routes used by BACE1 or APP...
  101. ncbi Kremen proteins are Dickkopf receptors that regulate Wnt/beta-catenin signalling
    Bingyu Mao
    Molecular Embryology Division, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Nature 417:664-7. 2002
    ..The results indicate that Kremen1 and Kremen2 are components of a membrane complex modulating canonical Wnt signalling through LRP6 in vertebrates...

Research Grants70

    J Evan Sadler; Fiscal Year: 2013
    ..Completion of this program will prepare talented trainees for careers in basic and translational hematology research, to make discoveries that will transform the diagnosis and treatment of hematologic diseases. ..
  2. Role of Endocytosis and Vesicular Trafficking in Podocyte Health and Filter Integ
    Puneet Garg; Fiscal Year: 2012
    Frances M Brodsky; Fiscal Year: 2013
  4. FASEB SRC on Mitochondrial Assembly &Dynamics in Health, Disease &Aging
    Antoni Barrientos; Fiscal Year: 2011
    ..Experts will also learn about involvement of mitochondrial function/dysfunction in common age-related neurodegenerative disorders and the aging process. ..
  5. Cx43 phosphorylation modulates Kras mediated pancreas cancer progression
    Paul D Lampe; Fiscal Year: 2012
    ..Since drugs that affect Cx43 activity and gap junctional communication are under development, understanding how these factors affect progression could ultimately lead to better treatment of this lethal disease. ..
    Stephan Wilkens; Fiscal Year: 2013
  7. Analyzing Anthrax Toxins in Drosophilia
    Ethan Bier; Fiscal Year: 2013
  8. Disease-related defects in dendritic cell processing of bacterial antigens
    Michael S Marks; Fiscal Year: 2012
    ..monocytogenes) in vivo. 2. To test whether LRO biogenesis and/or phagosome maturation in bone marrow-derived DCs (BMDCs) is altered in HPS mouse models lacking BLOC-1 or AP-3. ..
  9. Regulation and Function of the HCMV pp71 Tegument Protein
    Julie Kerry; Fiscal Year: 2009
    ..This research will analyze the function and regulation of viral proteins involved in establishing infection and assembling new virus particles, and thus may identify novel targets for anti-HCMV therapies. ..
  10. The Effects of Ethanol on Microtubules during Mouse Pre-implantation Development
    Yixian Zheng; Fiscal Year: 2012
  11. Laser Spinning Disc Confocal System for live-cell and live-organism microscopy
    Martha C Soto; Fiscal Year: 2012
    ..These researchers study essential processes of cells including how cells move, how cells like neurons are healed after injury, and how molecules are localized correctly to specific regions of cells. ..
  12. Enzymology of Post-translational Modifications
    Carol A Fierke; Fiscal Year: 2013
    ..Identification of the protein substrates and downstream biological function will enhance the development of new therapeutics targeting these pathways. ..
  13. Colonic Cytokinetics and Cell Signaling: Dietary Effects
    ROBERT STEPHEN CHAPKIN; Fiscal Year: 2012
  14. Pathogenic Mechanisms of Fatty Liver Disease
    Mohammad S Siddiqui; Fiscal Year: 2011
  15. Distance-Dependent Structure and Function of Neuronal Dendrites
    Kristen M Harris; Fiscal Year: 2013
    ..Investigative tools will be improved to measure core subcellular structures regulate synaptic load, especially during long-term potentiation, a well-studied cellular mechanism of learning and memory. ..
  16. Role of Protein Kinase D in Skin Epithelia
    Soosan Ghazizadeh; Fiscal Year: 2012
    ..functions including cell survival, proliferation, migration, differentiation, membrane trafficking and protein transport. Moreover, PKD1 is implicated in several pathological processes including stress-dependent cardiac ..
  17. Defining the role of Amot lipid binding in cellular proliferation and migration
    ANN CAROL KIMBLE-HILL; Fiscal Year: 2013
    ..Hence, all elements of this proposal (e.g. research, training, etc.) will be used o prepare and apply for a NIH R01 in the fourth year of the award to further her independent research status. ..
    Karlett J Parra; Fiscal Year: 2013
    ..As pathophysiology of the glucose-fatty acid cycle results in metabolic disorders including diabetes and chronic kidney disease, our studies will also contribute towards their understanding. ..
  19. Receptor-mediated Endocytosis-Mechanism and Function
    Frederick R Maxfield; Fiscal Year: 2013
    ..The proposed work Is divided in two main themes relating to membrane protein transport (Aims l-lll) and cholesterol and lipid transport (Aims IV and V)...
  20. Functional Studies of the Incretin GIP Receptor in Adipocytes
    Timothy E McGraw; Fiscal Year: 2013
    ..The GRKs and [unreadable]-arrestins control the internalization/down regulation of activated GPCR's, and therefore control signaling of the GPCRs. ..
  21. Molecular mechanisms of B cell malignant transformation
    Ping Xie; Fiscal Year: 2013
    ..In summary, this study will provide new insights into the exact mechanisms of TRAF3 inactivation-mediated oncogenic B cell survival, and will open up new avenues for the treatment of B lymphoma and MM. ..
  22. Membrane Protein Trafficking in Photoreceptors
    Wolfgang Baehr; Fiscal Year: 2013 an outer segment?" The proposed research examines aspects of integral membrane and peripheral membrane protein transport from photoreceptor inner segment (site of biosynthesis) to the outer segment (site of phototransduction)...
  23. Alveolar Cell Dysfunction and Pulmonary Fibrosis
    Lisa R Young; Fiscal Year: 2011
    ..Simply put, this proposal will use a powerful genetic mouse model to understand how scarring occurs in the lung, and how scarring in the lung might be prevented. (End of Abstract) ..
  24. Activation of rapamycin-sensitive TORC1 by endomembrane amino acid transporters
    MARIA E CARDENAS-CORONA; Fiscal Year: 2013
    ..Characterization of this pathway in yeast is continuing to reveal basic aspects of TORC1 signaling that could foster analogous studies in mammals and lead to improved anticancer therapies. ..
    Elizabeth C Engle; Fiscal Year: 2013
    ..abstract_text> ..
  26. Drs2p Function in Clathrin-coated Vesicle Budding
    Todd R Graham; Fiscal Year: 2013
    ..membrane asymmetry, these studies have surprisingly shown that P4-ATPases are required for vesicle-mediated protein transport in the secretory and endocytic pathways...
  27. Intraflagellar Transport Proteins in Mice
    Gregory J Pazour; Fiscal Year: 2013
    ..Understanding the mechanisms of ciliary membrane protein transport will provide insight into the etiology of many ciliary diseases since the sensory functions of cilia depend ..
  28. Alcohol Alters Hepatic Immune Function: Role of the Asialoglycoprotein Receptor
    Benita L McVicker; Fiscal Year: 2013
    ..Overall, this work aims to establish and characterize the role of the hepatic ASGP receptor in immune regulation and whether the alterations of this proce ..
  29. Mechanisms of Metal Ion Regulation in Human Cells
    Svetlana Lutsenko; Fiscal Year: 2013
    ..The proposed studies would elucidate the key aspects of copper regulation in human cells;that would facilitate the development of better therapies for copper-related disorders. ..
  30. Modulation of Rab7-Dependent Degradative Pathways by Novel Adenovirus Protein
    Cathleen R Carlin; Fiscal Year: 2012
    ..Altogether these studies will provide novel insights to the cell biology of membrane protein trafficking, and also the molecular basis for adenovirus-induced inflammatory disease. ..
  31. Characterization of WNK function in Axonal Maintenance
    Erin L Filbert; Fiscal Year: 2012
  32. Structural and Dynamical Response of Escherichia coli to Osmotic Stress
    James C Weisshaar; Fiscal Year: 2010
    ..In this project we study protein transport (diffusion) and the organization of proteins, DNA, and ribosomes within the cytoplasm of live cells using ..
  33. Pathogenesis of TSC2-Related Tumors
    Raymond Yeung; Fiscal Year: 2012
    ..function of mTOR in regulating microtubule organization, and hence cell morphology as well as its role in protein transport. In cells lacking hamartin or tuberin, products of TSC1 and TSC2, certain plasma membrane proteins are ..
  34. Gap Junction Trafficking to and within the Plasma Membrane
    ROBIN MARK SHAW; Fiscal Year: 2013
    ..This application proposes to study the molecular mechanisms of electrical coupling between heart cells and identify proteins involved in regulating the coupling under normal conditions and during times of reduced blood flow (ischemia). ..
    Nansi J Colley; Fiscal Year: 2013
    ..We anticipate that this work will greatly impact our understanding of the fundamental mechanisms of protein trafficking and also provide important insights into retinal diseases such as RP and AMD. ..
  36. SUMO1 and SERCA2a Function
    Roger J Hajjar; Fiscal Year: 2013
    ..SUMOylation has been found to be involved in many cellular processes such as protein transport, gene transcription and DNA replication and repair...
  37. Acquisition of a Leica TCS SP5 Confocal Microscope For A Multi-User Facility
    Patricia A Conrad; Fiscal Year: 2013
    ..All of these projects use methods of multiparameter mapping strategies in living and fixed cells and tissues and critically need the capabilities of th requested SP5 confocal for their NIH-funded projects to move forward. ..
  38. Targeting Endoplasmic Reticulum Stress Response for Cancer Therapy
    Bertal H Aktas; Fiscal Year: 2013
    ..binding protein-1 Xbp-1 that control expression of ER-chaperons, ER biogenesis and ER-associated retrograde protein transport and degradation genes...
  39. Vaccinia Virus Genetics and Morphogenesis
    Richard C Condit; Fiscal Year: 2013
    Peter Arvan; Fiscal Year: 2012
    ..This complexity generates several fundamental questions concerning intracellular protein transport and endoplasmic reticulum (ER) quality control function that link directly to clinical disease...
  41. Fat1 and Conventional Kinesin in Lateral Polarity
    DUNCAN BRUCE JOHNSTONE; Fiscal Year: 2012
    ..Fat1 research has applications to general cell biology, including cell polarity, intracellular protein transport, and protein-protein interactions...
  42. PtdIns 4-Kinase Regulation of Protein Sorting in the Golgi Apparatus
    Kathryn M Ferguson; Fiscal Year: 2013
  43. Automated Microscope for Long-Duration, Quantitative Dynamic Imaging
    JOHN PETER WIKSWO; Fiscal Year: 2010
  44. New Methods for the Synthesis of Neurological Agents and Other Heterocyclic Molec
    Larry E Overman; Fiscal Year: 2013 fragmented with localization of the fragments in the pericentriolar region with concurrent block of protein transport out of the Golgi...
  45. Ubiquitin-like Small Archaeal Protein Modification (SAMPylation)
    Julie A Maupin-Furlow; Fiscal Year: 2013
    ..Advancing our understanding of these processes will greatly assist in targeting proteasomes and Ub/Ubl-conjugation pathways for the development of new anti-cancer and anti-inflammatory drugs. ..
  46. Mechanisms of how nuclear envelope bridges link nuclei to the cytoskeleton.
    Daniel A Starr; Fiscal Year: 2013
    ..The proposed research is significant because it is expected to (A) elucidate mechanisms of protein transport to the inner nuclear membrane, (B) elucidate mechanisms of bi- directional nuclear migration along polarized ..
    Gregory S Payne; Fiscal Year: 2013
    ..The overall goal of this project is to understand the molecular basis of selective protein transport by ccv in normal cells to provide a foundation for understanding how defects can lead to disease...
  48. Membrane Remodeling and Protein Trafficking in Bacteria
    David Z Rudner; Fiscal Year: 2013
    ..Understanding the interplay between membrane proteins and the lipid bilayer in which they resides could lead to the discovery of new targets appropriate for antimicrobial therapeutics. ..
  49. Role of Ypt/Rab GTPases in intra-cellular Trafficking
    Nava Segev; Fiscal Year: 2013
    ..examined for their interaction with the Golgi Ypts, their effect on Ypt nucleotide switching in vitro, and protein transport and intracellular localization i vivo...
  50. Cell Biology, Genetics, and Biochemistry Training Program
    DAVID OWEN MORGAN; Fiscal Year: 2013
  51. Role of the novel gene fam4b in phosphatidylserine synthesis in podocytes and mai
    HUA A LU; Fiscal Year: 2012
    ..We proposed to examine the underlying mechanism of this gene and understand its contribution to the pathophysiology of "leaky" kidney diseases. ..
  52. Secretion, protein targeting, and lipid metabolism in African Trypanosomes
    James D Bangs; Fiscal Year: 2013
    ..provided by applicant): The goal of this proposal is to understand the related processes of secretory protein transport and lipid metabolism in African trypanosomes...
  53. Aging Research at the 2010 through 2014 ASHEcon Biennial Conferences
    Jody L Sindelar; Fiscal Year: 2013
    ..The subsequent meetings (2012 and 2014) will follow a similar format and will include topics highly relevant to the current research at the time of the meeting. ..
  54. Cytoskeletal regulation of intestinal barrier function and response to pathogens
    John Garber; Fiscal Year: 2013
    ..At the conclusion of the award period, the candidate will be well positioned for a career as an independent, R01-funded investigator. ..
  55. 2010 Cold Spring Harbor Laboratory Conference on Molecular Chaperones and Stress
    David J Stewart; Fiscal Year: 2012
    ..The subsequent meetings (2012 and 2014) will follow a similar format and will include topics highly relevant to the current research at the time of the meeting. ..
  56. EVC2: A novel modulator in tooth development
    Yoshiyuki Mochida; Fiscal Year: 2013
    ..The data obtained from this study will provide new insights into the pathophysiological function of EVC2 in ameloblast cell function and the molecular mechanism for tooth development. ..
  57. Role of PACS proteins in polycystin-2 trafficking and ADPKD
    Gary Thomas; Fiscal Year: 2010
  58. Mechanism of cholera toxin retro-translocation
    Billy Tsai; Fiscal Year: 2013
    ..Thus we anticipate that our findings are likely to reveal novel mechanisms of protein transport across biological membranes and to identify new cellular factors that may serve as viable therapeutic ..
  59. Protein Transport by the Bacterial Tat Machinery
    Siegfried M Musser; Fiscal Year: 2010
    ..Further, the ability of the Tat system to transport fully-folded protein complexes suggests that it will eventually find utility in the expression of protein therapeutics. ..
  60. The plant-like vacuole of Toxoplasma gondii
    Silvia N Moreno; Fiscal Year: 2013
    ..We think that the PLV plays a central role during the extracellular phase of the parasite not only in resisting environmental stress but also as it prepares itself for invading the next host cell. ..
  61. Alcohol-induced changes in hepatic microtubules: mechanisms and consequences
    Pamela L Tuma; Fiscal Year: 2012
    ..Of those deaths, greater than 20,000 are caused by cirrhosis of the liver, the seventh largest cause of death in Americans. Clearly, alcoholic liver disease is a major biomedical health concern in the United States. ..
  62. Molecular Mechanisms that Regulate Lysosomal Protein Transport
    Anjon Audhya; Fiscal Year: 2013
    ..multiple transmembrane proteins, providing an ideal, physiologically relevant system for studying lysosomal protein transport. C...
  63. SRP RNA level as a determinant of leishmanial parasitism of macrophages
    Gautam Chaudhuri; Fiscal Year: 2009
    ..Since the microbicidal functions of macrophages profoundly count on vesicular protein transport processes, down regulation of 7SL RNA may be significant in the establishment of infection by Leishmania...
  64. Clathrin adaptor function at the trans Golgi network and endosomes
    Gregory Payne; Fiscal Year: 2007
    ..cerevisiae indicate that clathrin coated vesicles (ccv) participate in evolutionarily conserved protein transport between the TGN and endosomes...
    Dennis Brown; Fiscal Year: 2006
    ..abstract_text> ..
  66. 2012 Intrinsically Disordered Proteins Gordon Research Conference
    Rohit V Pappu; Fiscal Year: 2012
    ..In choosing the recipients for support through NIH funds, the organizers will pay special attention to the need for drawing young scientists who are women and belong to under-represented minority groups. ..
    MICHAEL FLESSNER; Fiscal Year: 1999
    ..Recently proposed mathematical models of interstitial fluid and protein transport include theories of convection which are potentially capable of calculating interstitial pressure forces, ..
  68. Signaling Mechanisms in Drosophila Neural Development
    Kai Zinn; Fiscal Year: 2007
    ..We will also search for enhancers and suppressors of a bchs GOF eye phenotype. Candidate genes from the eye screen will be tested for modification of the bchs GOF neuromuscular phenotype and for interaction with bchs LOF mutations. ..
  69. Regulation of lung alveolar epithelial protein transport
    Kwang Jin Kim; Fiscal Year: 2005
    ..Preliminary studies on protein transport across primary cultured cell monolayers, which exhibit phenotypic and morphological traits of in vivo ..
    RICHARD HOLZ; Fiscal Year: 2002