- Tetherin inhibits retrovirus release and is antagonized by HIV-1 Vpu
Stuart J D Neil
Aaron Diamond AIDS Research Center and Laboratory of Retrovirology, The Rockefeller University, 455 First Avenue, New York, New York 10016, USA
Nature 451:425-30. 2008
..Vpu co-localized with CD317 and inhibited these effects. Inhibition of Vpu function and consequent mobilization of tetherin's antiviral activity is a potential therapeutic strategy in HIV/AIDS...
- Membrane lipids: where they are and how they behave
Gerrit van Meer
Bijvoet Center and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
Nat Rev Mol Cell Biol 9:112-24. 2008
..enzymes, translocases and transporters, plus the intrinsic physical phase behaviour of lipids and their interactions with membrane proteins, to create the unique compositions and multiple functionalities of their individual membranes?..
- Ceramide triggers budding of exosome vesicles into multivesicular endosomes
Centre for Biochemistry and Molecular Cell Biology, University of Gottingen, 37073 Gottingen, Germany
Science 319:1244-7. 2008
..Purified exosomes were enriched in ceramide, and the release of exosomes was reduced after the inhibition of neutral sphingomyelinases. These results establish a pathway in intraendosomal membrane transport and exosome formation...
- Development by self-digestion: molecular mechanisms and biological functions of autophagy
Department of Medicine, Columbia University, New York, NY 10032, USA
Dev Cell 6:463-77. 2004
..In this review, we summarize our current knowledge about the molecular machinery of autophagy and the role of the autophagic machinery in eukaryotic development...
- Local, efflux-dependent auxin gradients as a common module for plant organ formation
Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, 72076 Tubingen, Germany
Cell 115:591-602. 2003
..Our results suggest that PIN-dependent, local auxin gradients represent a common module for formation of all plant organs, regardless of their mature morphology or developmental origin...
- Parkin is recruited selectively to impaired mitochondria and promotes their autophagy
Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Biol 183:795-803. 2008
..These results show that Parkin promotes autophagy of damaged mitochondria and implicate a failure to eliminate dysfunctional mitochondria in the pathogenesis of Parkinson's disease...
- Drosophila pink1 is required for mitochondrial function and interacts genetically with parkin
Ira E Clark
Nature 441:1162-6. 2006
..The role of the pink1-parkin pathway in regulating mitochondrial function underscores the importance of mitochondrial dysfunction as a central mechanism of Parkinson's disease pathogenesis...
- The Wnt signaling pathway in development and disease
Catriona Y Logan
Department of Developmental Biology, Beckman Center, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
Annu Rev Cell Dev Biol 20:781-810. 2004
..The next few years are likely to see novel therapeutic reagents aimed at controlling Wnt signaling in order to alleviate these conditions...
- Exosomes--vesicular carriers for intercellular communication
Max Planck Institute for Experimental Medicine, Gottingen, Germany
Curr Opin Cell Biol 21:575-81. 2009
..Only recently light have been shed on the subcellular compartments and mechanisms involved in their biogenesis and secretion opening new avenues to understand their functions...
- One step at a time: endoplasmic reticulum-associated degradation
Shruthi S Vembar
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
Nat Rev Mol Cell Biol 9:944-57. 2008
..Here, we summarize our current understanding of each step during ERAD, with emphasis on the factors that catalyse distinct activities...
- A role for ubiquitin in selective autophagy
Institute of Biochemistry II and Cluster of Excellence Macromolecular Complexes, Goethe University, Theodor Stern Kai 7, Frankfurt, Germany
Mol Cell 34:259-69. 2009
..This review explores the hypothesis that ubiquitin represents a selective degradation signal suitable for targeting various types of cargo, ranging from protein aggregates to membrane-bound organelles and microbes...
- Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum
Elizabeth L Axe
Signalling Programme, Babraham Institute, Babraham, Cambridge, England, UK
J Cell Biol 182:685-701. 2008
..This PI(3)P-enriched compartment may be involved in autophagosome biogenesis. Its dynamic relationship with the ER is consistent with the idea that the ER may provide important components for autophagosome formation...
- The deacetylase HDAC6 regulates aggresome formation and cell viability in response to misfolded protein stress
Department of Pharmacology and Cancer Biology, Duke University, Durham, NC 27710, USA
Cell 115:727-38. 2003
..These findings identify HDAC6 as a crucial player in the cellular management of misfolded protein-induced stress...
- The ESCRT machinery in endosomal sorting of ubiquitylated membrane proteins
Centre for Cancer Biomedicine, Norwegian Radium Hospital, University of Oslo, Oslo, Norway
Nature 458:445-52. 2009
..The involvement of the ESCRT machinery in suppressing diseases such as cancer, neurodegeneration and infections underscores its importance to the cell...
- Drosophila parkin requires PINK1 for mitochondrial translocation and ubiquitinates mitofusin
Department of Biomedical Sciences, Medical Research Council Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield S10 2TN, United Kingdom
Proc Natl Acad Sci U S A 107:5018-23. 2010
..We hypothesize that Mfn ubiquitination may provide a mechanism by which terminally damaged mitochondria are labeled and sequestered for degradation by autophagy...
- Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
Cell 141:290-303. 2010
..Thus, Rag-Ragulator-mediated translocation of mTORC1 to lysosomal membranes is the key event in amino acid signaling to mTORC1...
- Importing mitochondrial proteins: machineries and mechanisms
Institut fur Biochemie und Molekularbiologie, ZBMZ, Universitat Freiburg, 79104 Freiburg, Germany
Cell 138:628-44. 2009
..Here, we discuss recent insights into the importing and sorting of mitochondrial proteins and their contributions to mitochondrial biogenesis...
- A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis
Maxence V Nachury
Genentech, Inc, South San Francisco, CA 94080, USA
Cell 129:1201-13. 2007
..Conversely, preventing Rab8(GTP) production blocks ciliation in cells and yields characteristic BBS phenotypes in zebrafish. Our data reveal that BBS may be caused by defects in vesicular transport to the cilium...
- Ciliogenesis: building the cell's antenna
Department of Biochemistry and Biophysics, University of California, San Francisco, BOX 2200 GH N372F, 600 16th Street, San Francisco, California 94158, USA
Nat Rev Mol Cell Biol 12:222-34. 2011
..As cilia are important in sensing and processing developmental signals and directing the flow of fluids such as mucus, defects in ciliogenesis and length control are likely to underlie a range of cilium-related human diseases...
- Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system
Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 103:1528-33. 2006
..Thus, the genes encoding the VAS-related, type VI secretion system likely play an important conserved function in microbial pathogenesis and represent an additional class of targets for vaccine and antimicrobial drug-based therapies...
- The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305 5345, USA
Cell 141:1208-19. 2010
..Thus, we propose that trafficking of BBSome cargoes to cilia entails the coupling of BBSome coat polymerization to the recognition of sorting signals by the BBSome...
- Nonproteolytic functions of ubiquitin in cell signaling
Zhijian J Chen
Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
Mol Cell 33:275-86. 2009
..Recent advances in understanding ubiquitination in protein kinase activation and DNA repair are discussed to illustrate the nonproteolytic functions of ubiquitin in cell signaling...
- The AAA ATPase Cdc48/p97 and its partners transport proteins from the ER into the cytosol
Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 414:652-6. 2001
..We propose that the Cdc48/p97-Ufd1-Npl4 complex extracts proteins from the ER membrane for cytosolic degradation...
- Amyloid precursor protein trafficking, processing, and function
Department of Neurobiology, The University of Chicago, Chicago, Illinois 60637, USA
J Biol Chem 283:29615-9. 2008
..This minireview summarizes salient features of APP trafficking and amyloidogenic processing and discusses the putative biological functions of APP...
- Impaired degradation of mutant alpha-synuclein by chaperone-mediated autophagy
Ana Maria Cuervo
Department of Anatomy and Structural Biology, Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA
Science 305:1292-5. 2004
..These findings may underlie the toxic gain-of-function by the mutants...
- Global analysis of protein localization in budding yeast
Won Ki Huh
Howard Hughes Medical Institute, University of California San Francisco, Department of Biochemistry and Biophysics, 600 16th Street, San Francisco, California 94143 2240, USA
Nature 425:686-91. 2003
- GLUT4 exocytosis
Diabetes and Obesity Program, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia
J Cell Sci 124:4147-59. 2011
..This regulatory process clearly involves the Ser/Thr kinase AKT and the exquisite manner in which this single metabolic process is regulated makes it a likely target for lesions that might contribute to metabolic disease...
- Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins
Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Bunkyo ku, Tokyo, Japan
Autophagy 6:764-76. 2010
..These hierarchical analyses suggest that ULK1, Atg14 and VMP1 localize to the ER-associated autophagosome formation sites in a PI3-kinase activity-independent manner...
- Integrins: masters and slaves of endocytic transport
Patrick T Caswell
Beatson Institute for Cancer Research, Cancer Research UK, Glasgow, G61 3BX, Scotland
Nat Rev Mol Cell Biol 10:843-53. 2009
..Furthermore, integrins can exert control over the trafficking of other receptors in a way that drives cancer cell invasion and tumour angiogenesis...
- Protein translocation across the bacterial cytoplasmic membrane
Arnold J M Driessen
Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute for Advanced Materials, University of Groningen, Haren, The Netherlands
Annu Rev Biochem 77:643-67. 2008
..This review summarizes the present knowledge of the mechanism and structure of the Sec translocase, with a special emphasis on unresolved questions and topics of current research...
- The clathrin adaptor AP-1A mediates basolateral polarity
Margaret Dyson Vision Research Institute, Department of Ophthalmology, Weill Cornell Medical College, New York, NY 10065, USA
Dev Cell 22:811-23. 2012
..The basolateral sorting function of AP-1A reported here establishes AP-1 as a major regulator of epithelial polarity...
- SNX27 mediates retromer tubule entry and endosome-to-plasma membrane trafficking of signalling receptors
Department of Psychiatry, University of California at San Francisco, San Francisco, California 94158, USA
Nat Cell Biol 13:715-21. 2011
..The present results identify a role for retromer in endocytic trafficking of signalling receptors, in regulating a receptor-linked signalling pathway, and in mediating direct endosome-to-plasma membrane traffic...
- Synaptic AMPA receptor plasticity and behavior
Helmut W Kessels
Department of Neuroscience, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
Neuron 61:340-50. 2009
..We argue that monitoring and manipulating synaptic AMPAR trafficking represents an attractive means to study cognitive function and dysfunction in animal models...
- PINK1-dependent recruitment of Parkin to mitochondria in mitophagy
Department of Neurology, Columbia University, New York, NY 10032, USA
Proc Natl Acad Sci U S A 107:378-83. 2010
..Thus by impairing this process, mutations in either Parkin or PINK1 may alter mitochondrial turnover which, in turn, may cause the accumulation of defective mitochondria and, ultimately, neurodegeneration in Parkinson's disease...
- Mitochondrial protein import: from proteomics to functional mechanisms
Institut fur Biochemie und Molekularbiologie, ZBMZ, Universitat Freiburg, Stefan Meier Strabetae 17, 79104 Freiburg, Germany
Nat Rev Mol Cell Biol 11:655-67. 2010
..Protein translocases do not function as independent units but are integrated into dynamic networks and are connected to machineries that function in bioenergetics, mitochondrial morphology and coupling to the endoplasmic reticulum...
- A translocation signal for delivery of oomycete effector proteins into host plant cells
Stephen C Whisson
Plant Pathology Programme, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK
Nature 450:115-8. 2007
..infestans genome. Identification of this class of proteins provides unparalleled opportunities to determine how oomycetes manipulate hosts to establish infection...
- Coordinated protein sorting, targeting and distribution in polarized cells
Genentech, Inc, 1 DNA Way, South San Francisco, California 94080, USA
Nat Rev Mol Cell Biol 9:833-45. 2008
..The integration of these mechanisms into a complex and dynamic network is crucial for normal tissue function and is often defective in disease states...
- GTPase networks in membrane traffic
Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371 8512, Japan
Annu Rev Biochem 81:637-59. 2012
..As each GTPase recruits a unique set of effectors, these transitions help to define changes in the functionality of the membrane compartments with which they are associated...
- Vinculin controls focal adhesion formation by direct interactions with talin and actin
Jonathan D Humphries
Wellcome Trust Centre for Cell Matrix Research, University of Manchester, Manchester M13 9PT, England, UK
J Cell Biol 179:1043-57. 2007
..The vinculin tail, however, is responsible for the functional link of FAs to the actin cytoskeleton. We propose a new model that explains how vinculin orchestrates FAs...
- Prions hijack tunnelling nanotubes for intercellular spread
Unité de Trafic Membranaire et Pathogénèse, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
Nat Cell Biol 11:328-36. 2009
..We therefore propose that TNTs are involved in the spreading of PrP(Sc) within neurons in the CNS and from the peripheral site of entry to the PNS by neuroimmune interactions with dendritic cells...
- The Atg16L complex specifies the site of LC3 lipidation for membrane biogenesis in autophagy
Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
Mol Biol Cell 19:2092-100. 2008
..We propose that the Atg16L complex is a new type of E3-like enzyme that functions as a scaffold for LC3 lipidation by dynamically localizing to the putative source membranes for autophagosome formation...
- Colicin biology
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Microbiol Mol Biol Rev 71:158-229. 2007
..The mechanisms of inhibition by specific and cognate immunity proteins are presented. Finally, the use of colicins as laboratory or biotechnological tools and their mode of evolution are discussed...
- Road to ruin: targeting proteins for degradation in the endoplasmic reticulum
Melanie H Smith
Department of Cellular and Molecular Pharmacology, Graduate Group in Biophysics and Howard Hughes Medical Institute, University of California San Francisco, CA 94158, USA
Science 334:1086-90. 2011
..Much less is known about the features used to identify terminally misfolded conformations and the broader role of these pathways in regulating protein half-lives...
- Molecular motors in neurons: transport mechanisms and roles in brain function, development, and disease
Departments of Cell Biology and Anatomy and Molecular Structure and Dynamics, Graduate School of Medicine, University of Tokyo, Hongo, Tokyo 113 0033, Japan
Neuron 68:610-38. 2010
..Finally, it is also evident that molecular motors are critically involved in neuronal disease pathogenesis. Thus, molecular motor research is becoming an exciting frontier of neuroscience...
- Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy
Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
Mol Biol Cell 20:1981-91. 2009
..ULK1 is dephosphorylated by rapamycin treatment or starvation. These data suggest that mTORC1 suppresses autophagy through direct regulation of the approximately 3-MDa ULK1-Atg13-FIP200 complex...
- Endocytosis and intracellular trafficking of ErbBs
Department of Pharmacology, University of Colorado Denver Anschutz Medical Center, Aurora, Colorado 80045 0508, USA
Exp Cell Res 315:683-96. 2009
..However, these ErbBs are internalized and targeted to lysosomes less efficiently than EGFR. When overexpressed endocytosis-impaired ErbBs may inhibit the internalization and degradation of EGFR...
- Cytokinin modulates endocytic trafficking of PIN1 auxin efflux carrier to control plant organogenesis
Department of Plant Systems Biology, VIB, Technologiepark 927, B 9052 Gent, Belgium
Dev Cell 21:796-804. 2011
- The transcellular spread of cytosolic amyloids, prions, and prionoids
Institute of Neuropathology, University Hospital of Zurich, Schmelzbergstrasse 12, CH 8091 Zurich, Switzerland
Neuron 64:783-90. 2009
- Adapting proteostasis for disease intervention
William E Balch
Department of Cell Biology and Institute for Childhood and Neglected Diseases, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Science 319:916-9. 2008
- Exosomes: a common pathway for a specialized function
Guillaume van Niel
Institut Curie, CNRS UMR144, 75248 Paris Cedex, France
J Biochem 140:13-21. 2006
..These findings open up new therapeutic and diagnostic strategies...
- Mechanisms of regulated unconventional protein secretion
Heidelberg University Biochemistry Center, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
Nat Rev Mol Cell Biol 10:148-55. 2009
- Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources?
CEA, iRTSV, Laboratoire Biologie, Informatique et Mathématiques, Grenoble, France
BMC Genomics 10:104. 2009
..By combining comparative and phylogenetic approaches using more than 500 prokaryotic genomes, we characterized the global T6SS genetic structure in terms of conservation, evolution and genomic organization...
- The mouse Spo11 gene is required for meiotic chromosome synapsis
P J Romanienko
Genetics and Biochemistry Branch National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health 20892, Bethesda, MD, USA
Mol Cell 6:975-87. 2000
..We speculate that there is an additional role for Spo11, after it generates DSBs, in synapsis...
- Toxoplasma sortilin-like receptor regulates protein transport and is essential for apical secretory organelle biogenesis and host infection
Pierre Julien Sloves
Center for Infection and Immunity of Lille, Universite Lille Nord de France, Lille, France
Cell Host Microbe 11:515-27. 2012
..the cytoplasmic tail of TgSORTLR recruits cytosolic sorting machinery involved in anterograde and retrograde protein transport. Ectopic expression of the N-terminal TgSORTLR lumenal domain results in dominant negative effects with the ..
- The ubiquitin-selective segregase VCP/p97 orchestrates the response to DNA double-strand breaks
Institute of Pharmacology and Toxicology, University of Zurich Vetsuisse, Winterthurerstrasse 260, 8057 Zurich, Switzerland
Nat Cell Biol 13:1376-82. 2011
..These findings identify the p97-UFD1-NPL4 complex as an essential factor in ubiquitin-governed DNA-damage response, highlighting its importance in guarding genome stability...
- Ubiquitin-dependent regulation of COPII coat size and function
Department of Molecular and Cell Biology, University of California at Berkeley, California 94720, USA
Nature 482:495-500. 2012
..As a result, ubiquitylation by CUL3-KLHL12 is essential for collagen export, yet less important for the transport of small cargo. We conclude that monoubiquitylation controls the size and function of a vesicle coat...
- Non-classical protein secretion in bacteria
Jannick D Bendtsen
Center for Biological Sequence Analysis, BioCentrum DTU, Building 208, Technical University of Denmark, DK 2800 Lyngby, Denmark
BMC Microbiol 5:58. 2005
..Some of these proteins also have known roles in the cytoplasm, which means they could be so-called "moon-lightning" proteins having more than one function...
- Regulation of signaling protein function and trafficking by the hsp90/hsp70-based chaperone machinery
William B Pratt
Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0632, USA
Exp Biol Med (Maywood) 228:111-33. 2003
..This purified system should facilitate understanding of how eukaryotic hsp70 and hsp90 work together as essential components of a process that alters the conformations of substrate proteins to states that respond in signal transduction...
- Strange as it may seem: the many links between Wnt signaling, planar cell polarity, and cilia
John B Wallingford
Howard Hughes Medical Institute, Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA
Genes Dev 25:201-13. 2011
- The genome of the African trypanosome Trypanosoma brucei
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
Science 309:416-22. 2005
..brucei and the greatest in L. major. Horizontal transfer of genes of bacterial origin has contributed to some of the metabolic differences in these parasites, and a number of novel potential drug targets have been identified...
- Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis
Max Delbruck Laboratorium in der Max Planck Gesellschaft, 50829 Köln, Germany
Nature 415:806-9. 2002
..In addition, actin-dependent relocalization of PIN3 in response to gravity provides a mechanism for redirecting auxin flux to trigger asymmetric growth...
- Planar polarized actomyosin contractile flows control epithelial junction remodelling
IBDML, UMR6216 CNRS Université de la Méditerranée, Campus de Luminy, Case 907, 13288 Marseille Cedex 09, France
Nature 468:1110-4. 2010
..Thus, epithelial morphogenesis is not properly reflected by Myosin II steady state distribution but by polarized contractile actomyosin flows that emerge from interactions between E-cadherin and actomyosin networks...
- Nuclear trafficking of the epidermal growth factor receptor family membrane proteins
Y N Wang
Department of Molecular and Cellular Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
Oncogene 29:3997-4006. 2010
..Understanding the trafficking mechanisms as to how RTKs are transported from the cell surface to the nucleus will significantly contribute to understanding the functions of the nuclear RTKs...
- Arc/Arg3.1 regulates an endosomal pathway essential for activity-dependent β-amyloid generation
Solomon H Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Cell 147:615-28. 2011
..Genetic deletion of Arc reduces Aβ load in a transgenic mouse model of AD. In concert with the finding that patients with AD can express anomalously high levels of Arc, we hypothesize that Arc participates in the pathogenesis of AD...
- Intracellular functions of N-linked glycans
Institute of Biochemistry, , , , Switzerland
Science 291:2364-9. 2001
..The division of synthesis and processing between the ER and the Golgi complex represents an evolutionary adaptation that allows efficient exploitation of the potential of oligosaccharides...
- Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein
Olav M Andersen
Max Delbrueck Center for Molecular Medicine, D 13125 Berlin, Germany
Proc Natl Acad Sci U S A 102:13461-6. 2005
..Consequently, reduced receptor expression in the human brain may increase Abeta production and plaque formation and promote spontaneous AD...
- Exosomes: from biogenesis and secretion to biological function
German Cancer Research Center DKFZ, Tumor Immunology Program, D010 TP3, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Immunol Lett 107:102-8. 2006
- A bacterial virulence protein suppresses host innate immunity to cause plant disease
Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA
Science 313:220-3. 2006
..HopM1 mediates the destruction of AtMIN7 via the host proteasome. Our results illustrate a strategy by which a bacterial pathogen exploits the host proteasome to subvert host immunity and causes infection in plants...
- Rab25 associates with alpha5beta1 integrin to promote invasive migration in 3D microenvironments
Patrick T Caswell
Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
Dev Cell 13:496-510. 2007
..These data indicate that Rab25 contributes to tumor progression by directing the localization of integrin-recycling vesicles and thereby enhancing the ability of tumor cells to invade the extracellular matrix...
- Cullin3-based polyubiquitination and p62-dependent aggregation of caspase-8 mediate extrinsic apoptosis signaling
Department of Molecular Oncology, Genentech, Inc, 1 DNA Way, South San Francisco, CA 94080, USA
Cell 137:721-35. 2009
..These results identify a mechanism that positively controls apoptosis signaling by polyubiquitination and aggregation of a key initiator caspase...
- Cryo-EM structure of the ribosome-SecYE complex in the membrane environment
Gene Center, Department for Biochemistry, University of Munich, Munich, Germany
Nat Struct Mol Biol 18:614-21. 2011
..On the basis of our map and molecular dynamics simulations, we present a model of a signal anchor-gated PCC in the membrane...
- Localization of organellar proteins in Plasmodium falciparum using a novel set of transfection vectors and a new immunofluorescence fixation method
Christopher J Tonkin
Plant Cell Biology Research Centre, School of Botany, The University of Melbourne, Parkville, VIC 3010, Australia
Mol Biochem Parasitol 137:13-21. 2004
- The type VI secretion toolkit
Laboratoire d Ingenierie des Systemes Macromoleculaires, IBSM, Centre National de la Recherche Scientifique, Marseille, France
EMBO Rep 9:735-41. 2008
..This review provides an overview of our current knowledge about type VI secretion, focusing on gene regulation, components of the secretion machine, substrate secretion and the cellular consequences for the host cell...
- Cleaning up: ER-associated degradation to the rescue
Jeffrey L Brodsky
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
Cell 151:1163-7. 2012
..This process is termed endoplasmic-reticulum (ER)-associated degradation (ERAD)...
- Ankyrin repeat proteins comprise a diverse family of bacterial type IV effectors
Section of Microbial Pathogenesis, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
Science 320:1651-4. 2008
..pneumophila-containing vacuole with late endosomes after infection of macrophages, which demonstrates that Ank proteins have effector functions important for bacterial infection of eukaryotic host cells...
- Molecular mechanism of multivesicular body biogenesis by ESCRT complexes
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services, Bethesda, Maryland 20892, USA
Nature 464:864-9. 2010
..The observations explain how the ESCRTs direct membrane budding and scission from the cytoplasmic side of the bud without being consumed in the reaction...
- Protein sorting receptors in the early secretory pathway
Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
Annu Rev Biochem 79:777-802. 2010
..Our current understanding of the molecular mechanisms underlying cargo receptor function are described...
- A membrane protein complex mediates retro-translocation from the ER lumen into the cytosol
Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
Nature 429:841-7. 2004
..Derlin-1 interacts with US11, a virally encoded ER protein that specifically targets MHC class I heavy chains for export from the ER, as well as with VIMP, a novel membrane protein that recruits the p97 ATPase and its cofactor...
- Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation
Hannah E J Polson
Secretory Pathways Laboratory, London Research Institute, Cancer Research UK, London, UK
Autophagy 6:506-22. 2010
..In view of our data we propose a role for WIPI2 in the progression of omegasomes into autophagosomes...
- A photoactivatable GFP for selective photolabeling of proteins and cells
George H Patterson
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Science 297:1873-7. 2002
..Here, we use the photoactivatable GFP both as a free protein to measure protein diffusion across the nuclear envelope and as a chimera with a lysosomal membrane protein to demonstrate rapid interlysosomal membrane exchange...
- A transcriptionally [correction of transcriptively] active complex of APP with Fe65 and histone acetyltransferase Tip60
The Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9111 USA
Science 293:115-20. 2001
..This complex potently stimulates transcription via heterologous Gal4- or LexA-DNA binding domains, suggesting that release of the cytoplasmic tail of APP by gamma-cleavage may function in gene expression...
- Comprehensive identification of protein substrates of the Dot/Icm type IV transporter of Legionella pneumophila
Department of Biological Sciences, Purdue University, West Lafayette, Indiana, United States of America
PLoS ONE 6:e17638. 2011
..Thus, our efforts have identified a large number of novel substrates of the Dot/Icm system and have revealed the diverse features recognizable by this protein transporter...
- Identification of a tumour suppressor network opposing nuclear Akt function
Lloyd C Trotman
Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, Sloan Kettering Institute, 1275 York Avenue, New York, New York 10021, USA
Nature 441:523-7. 2006
..Our results demonstrate that Pml orchestrates a nuclear tumour suppressor network for inactivation of nuclear pAkt, and thus highlight the importance of AKT compartmentalization in human cancer pathogenesis and treatment...
- Generation of cell polarity in plants links endocytosis, auxin distribution and cell fate decisions
Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
Nature 456:962-6. 2008
..It also highlights the link between endocytosis-dependent polarity of individual cells and auxin distribution-dependent cell fate establishment for multicellular patterning...
- The mitochondrial p53 pathway
Angelina V Vaseva
Department of Pathology, Stony Brook University, Stony Brook, NY 11794, USA
Biochim Biophys Acta 1787:414-20. 2009
..Our review will discuss these studies, focusing on recent advances in the field...
- Rab-coupling protein coordinates recycling of alpha5beta1 integrin and EGFR1 to promote cell migration in 3D microenvironments
Patrick T Caswell
Beatson Institute for Cancer Research, Bearsden, Glasgow G61 1BD, Scotland, UK
J Cell Biol 183:143-55. 2008
..We conclude that RCP provides a scaffold that promotes the physical association and coordinate trafficking of alpha5beta1 and EGFR1 and that this drives migration of tumor cells into three-dimensional matrices...
- Analysis of nucleolar protein dynamics reveals the nuclear degradation of ribosomal proteins
Yun Wah Lam
Division of Gene Regulation and Expression, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
Curr Biol 17:749-60. 2007
- Phosphorylation of Bax Ser184 by Akt regulates its activity and apoptosis in neutrophils
Shyra J Gardai
Program in Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206, USA
J Biol Chem 279:21085-95. 2004
- Genome-scale identification of Legionella pneumophila effectors using a machine learning approach
Department of Cell Research and Immunology, George S Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel
PLoS Pathog 5:e1000508. 2009
..pneumophila effectors, predicted over a hundred additional highly probable effectors, and shown the applicability of machine learning algorithms for the identification and characterization of bacterial pathogenesis determinants...
- Spatial control of EGF receptor activation by reversible dimerization on living cells
Genentech, Inc, 1 DNA Way, South San Francisco, California 94080, USA
Nature 464:783-7. 2010
..Interestingly, dimers were enriched in the cell periphery in an actin- and receptor-expression-dependent fashion, resulting in a peripheral enhancement of EGF-induced signalling that may enable polarized responses to growth factors...
- Stats: transcriptional control and biological impact
David E Levy
Department of Pathology, New York University School of Medicine, New York, New York 10016, USA
Nat Rev Mol Cell Biol 3:651-62. 2002
..In addition to several roles in normal cell decisions, dysregulation of STAT function contributes to human disease, making the study of these proteins an important topic of current research...
- Trafficking to the ciliary membrane: how to get across the periciliary diffusion barrier?
Maxence V Nachury
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305 5345, USA
Annu Rev Cell Dev Biol 26:59-87. 2010
..Finally, some proteins need to exit from cilia, and ubiquitination may regulate this step. The stage is now set to dissect the interplay between signaling and regulated trafficking to and from cilia...
- PML regulates p53 stability by sequestering Mdm2 to the nucleolus
Cancer Biology and Genetics Program, Department of Pathology and Medicine, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
Nat Cell Biol 6:665-72. 2004
..These findings demonstrate an unexpected role of PML in the nucleolar network for tumour suppression...
- Membrane protein architects: the role of the BAM complex in outer membrane protein assembly
Timothy J Knowles
Cancer Research UK Institute for Cancer Studies, School of Cancer Sciences, Edgbaston, Birmingham, UK
Nat Rev Microbiol 7:206-14. 2009
..Here, we discuss how these structures illuminate the mechanisms responsible for the biogenesis of outer membrane proteins...
- Activity-dependent regulation of dendritic synthesis and trafficking of AMPA receptors
Nancy Friend Pritzker Laboratory, Department of Psychiatry and Behavioral Sciences, 1201 Welch Road, Stanford University School of Medicine, Palo Alto, California 94304, USA
Nat Neurosci 7:244-53. 2004
- A mechanism for vertebrate Hedgehog signaling: recruitment to cilia and dissociation of SuFu-Gli protein complexes
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
J Cell Biol 191:415-28. 2010
..Our results support a simple mechanism in which Hh signals at vertebrate cilia cause dissociation of inactive SuFu-Gli complexes, a process inhibited by PKA...
- A FAM21-containing WASH complex regulates retromer-dependent sorting
Timothy S Gomez
Department of Immunology, Division of Oncology Research and Schulze Center for Novel Therapeutics, College of Medicine, Mayo Clinic, Rochester, MN 55905, USA
Dev Cell 17:699-711. 2009
- Routes, destinations and delays: recent advances in AMPA receptor trafficking
Jeremy M Henley
MRC Centre for Synaptic Plasticity, School of Biochemistry, University of Bristol, University Walk, Bristol, UK, BS8 1TD
Trends Neurosci 34:258-68. 2011
..Here, we review recent findings that have provided new insights into AMPAR trafficking and that might lead to the development of novel therapeutic strategies...
- Antagonism of tetherin restriction of HIV-1 release by Vpu involves binding and sequestration of the restriction factor in a perinuclear compartment
Laboratory of Human Retrovirology, Institut de recherches cliniques de Montreal IRCM, Montreal, Quebec, Canada
PLoS Pathog 6:e1000856. 2010
- ADP ribosylation factor 6 (ARF6) controls amyloid precursor protein (APP) processing by mediating the endosomal sorting of BACE1
Laboratory of Membrane Trafficking, Center for Human Genetics, Catholic University of Leuven, Leuven, Belgium
Proc Natl Acad Sci U S A 108:E559-68. 2011
..It thereby provides a novel avenue to interfere with Aβ production through a selective modulation of the distinct endosomal transport routes used by BACE1 or APP...
- Kremen proteins are Dickkopf receptors that regulate Wnt/beta-catenin signalling
Molecular Embryology Division, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Nature 417:664-7. 2002
..The results indicate that Kremen1 and Kremen2 are components of a membrane complex modulating canonical Wnt signalling through LRP6 in vertebrates...