cell nucleus active transport

Summary

Summary: Gated transport mechanisms by which proteins or RNA are moved across the NUCLEAR MEMBRANE.

Top Publications

  1. pmc Nuclear targeting by the growth factor midkine
    Yoshihisa Shibata
    Department of Biochemistry, Nagoya University School of Medicine, Showa Ku, Japan
    Mol Cell Biol 22:6788-96. 2002
  2. ncbi Karyopherins: from nuclear-transport mediators to nuclear-function regulators
    Nima Mosammaparast
    Center for Cell Signaling and Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
    Trends Cell Biol 14:547-56. 2004
  3. pmc Structural basis for leucine-rich nuclear export signal recognition by CRM1
    Xiuhua Dong
    Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, 6001 Forest Park, Dallas, Texas 75390 9041, USA
    Nature 458:1136-41. 2009
  4. ncbi Spatial control of cytokinesis by Cdr2 kinase and Mid1/anillin nuclear export
    Maria Almonacid
    Institut Curie, Centre de Recherche, F 75248 Paris, France
    Curr Biol 19:961-6. 2009
  5. ncbi Analysis and prediction of leucine-rich nuclear export signals
    Tanja la Cour
    Center for Biological Sequence Analysis, BioCentrum DTU, Technical University of Denmark, Building 208, DK 2800 Lyngby, Denmark
    Protein Eng Des Sel 17:527-36. 2004
  6. ncbi Duration of nuclear NF-kappaB action regulated by reversible acetylation
    - Chen Lf
    Gladstone Institute of Virology and Immunology, Department of Medicine, University of California, San Francisco, CA 94141, USA
    Science 293:1653-7. 2001
  7. pmc Capsid is a dominant determinant of retrovirus infectivity in nondividing cells
    Masahiro Yamashita
    Division of Human Biology, Mail Stop C2 023, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave North, P O Box 19024, Seattle, WA 98109 1024, USA
    J Virol 78:5670-8. 2004
  8. ncbi Shaping the nuclear action of NF-kappaB
    Lin Feng Chen
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, California 94141, USA
    Nat Rev Mol Cell Biol 5:392-401. 2004
  9. ncbi Oscillations in NF-kappaB signaling control the dynamics of gene expression
    D E Nelson
    Centre for Cell Imaging, School of Biological Sciences, Bioscience Research Building, Crown Street, Liverpool, L69 7ZB, UK
    Science 306:704-8. 2004
  10. ncbi Exporting RNA from the nucleus to the cytoplasm
    Alwin Köhler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Nat Rev Mol Cell Biol 8:761-73. 2007

Research Grants

  1. HIV-1 TROPISM AND CHEMOKINE RECEPTORS
    BRYAN CULLEN; Fiscal Year: 2002
  2. Nucleocytoplasmic Trafficking of Smads
    Lan Xu; Fiscal Year: 2008
  3. Regulation of human microRNA biosynthesis and activity
    BRYAN CULLEN; Fiscal Year: 2007
  4. Effect of APOBEC3G on retroviruses and retrotransposons
    BRYAN CULLEN; Fiscal Year: 2009
  5. Molecular Mechanism of HIV-1 Vif Function
    BRYAN CULLEN; Fiscal Year: 2007
  6. DUX4 and Facioscapulohumeral Muscular Dystrophy
    Howard Worman; Fiscal Year: 2004
  7. High Through-Put Genomics Approach To Lifespan in Yeast
    David Goldfarb; Fiscal Year: 2006
  8. Lamin A Mutation and Hutchinson-Gilford Progeria
    Howard Worman; Fiscal Year: 2008
  9. Study of novel REST/NRSF-Interacting LIM domain Protein
    Masahito Shimojo; Fiscal Year: 2005
  10. GLOMERULAR LAMININS: STRUCTURE AND FUNCTION
    Christine Abrass; Fiscal Year: 2003

Detail Information

Publications273 found, 100 shown here

  1. pmc Nuclear targeting by the growth factor midkine
    Yoshihisa Shibata
    Department of Biochemistry, Nagoya University School of Medicine, Showa Ku, Japan
    Mol Cell Biol 22:6788-96. 2002
    ..This study therefore reveals a novel role of LRP in intracellular signaling by its ligand and the importance of nucleolin in this process...
  2. ncbi Karyopherins: from nuclear-transport mediators to nuclear-function regulators
    Nima Mosammaparast
    Center for Cell Signaling and Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
    Trends Cell Biol 14:547-56. 2004
    ....
  3. pmc Structural basis for leucine-rich nuclear export signal recognition by CRM1
    Xiuhua Dong
    Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, 6001 Forest Park, Dallas, Texas 75390 9041, USA
    Nature 458:1136-41. 2009
    ..Similar energetic construction is also used in multipartite nuclear localization signals to provide broad substrate specificity and rapid evolution in nuclear transport...
  4. ncbi Spatial control of cytokinesis by Cdr2 kinase and Mid1/anillin nuclear export
    Maria Almonacid
    Institut Curie, Centre de Recherche, F 75248 Paris, France
    Curr Biol 19:961-6. 2009
    ..We conclude that Cdr2- and nuclear export-dependent positioning of Mid1 constitute two overlapping mechanisms that relay cell polarity and nuclear positional information to ensure proper division-plane specification...
  5. ncbi Analysis and prediction of leucine-rich nuclear export signals
    Tanja la Cour
    Center for Biological Sequence Analysis, BioCentrum DTU, Technical University of Denmark, Building 208, DK 2800 Lyngby, Denmark
    Protein Eng Des Sel 17:527-36. 2004
    ..We employ both neural networks and hidden Markov models in the prediction algorithm and verify the method on the most recently discovered NESs. The NES predictor (NetNES) is made available for general use at http://www.cbs.dtu.dk/...
  6. ncbi Duration of nuclear NF-kappaB action regulated by reversible acetylation
    - Chen Lf
    Gladstone Institute of Virology and Immunology, Department of Medicine, University of California, San Francisco, CA 94141, USA
    Science 293:1653-7. 2001
    ....
  7. pmc Capsid is a dominant determinant of retrovirus infectivity in nondividing cells
    Masahiro Yamashita
    Division of Human Biology, Mail Stop C2 023, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave North, P O Box 19024, Seattle, WA 98109 1024, USA
    J Virol 78:5670-8. 2004
    ..These results suggest that CA is the dominant determinant for the difference between HIV and MLV in the ability to transduce nondividing cells...
  8. ncbi Shaping the nuclear action of NF-kappaB
    Lin Feng Chen
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, California 94141, USA
    Nat Rev Mol Cell Biol 5:392-401. 2004
  9. ncbi Oscillations in NF-kappaB signaling control the dynamics of gene expression
    D E Nelson
    Centre for Cell Imaging, School of Biological Sciences, Bioscience Research Building, Crown Street, Liverpool, L69 7ZB, UK
    Science 306:704-8. 2004
    ..The functional consequences of NF-kappaB signaling may thus depend on number, period, and amplitude of oscillations...
  10. ncbi Exporting RNA from the nucleus to the cytoplasm
    Alwin Köhler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Nat Rev Mol Cell Biol 8:761-73. 2007
    ..Export of mRNAs is unique as it is extensively coupled to transcription (in yeast) and splicing (in metazoa). Understanding the mechanisms that connect RNP formation with export is a major challenge in the field...
  11. ncbi Nucleocytoplasmic shuttling of Smad proteins
    Caroline S Hill
    Laboratory of Developmental Signalling, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Cell Res 19:36-46. 2009
    ..Finally, the biological relevance of continuous nucleocytoplasmic shuttling for signaling by TGF-beta superfamily members is discussed...
  12. ncbi Missing pieces in the NF-kappaB puzzle
    Sankar Ghosh
    Section of Immunobiology, Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06520, USA
    Cell 109:S81-96. 2002
    ..A critical component in NF-kappaB regulation is the IkappaB kinase (IKK) complex. This review is focused on recent progress as well as unanswered questions regarding the regulation and function of NF-kappaB and IKK...
  13. ncbi Leucine-rich nuclear-export signals: born to be weak
    Ulrike Kutay
    Swiss Federal Institute of Technology ETH Zurich, Institute of Biochemistry, Schafmattstrasse 18, HPM F11 1, 8093 Zurich, Switzerland
    Trends Cell Biol 15:121-4. 2005
    ..This finding suggests that high-affinity NES binding to CRM1 impairs the efficient release of export complexes from the NPC, explaining why leucine-rich NESs have evolved to be weak...
  14. ncbi The molecular architecture of the nuclear pore complex
    Frank Alber
    Department of Bioengineering and Therapeutic Sciences, and California Institute for Quantitative Biosciences, Mission Bay QB3, 1700 4th Street, Suite 503B, University of California at San Francisco, San Francisco, California 94158 2330, USA
    Nature 450:695-701. 2007
    ..These findings provide clues to the evolutionary origins of the NPC...
  15. pmc Rules for nuclear localization sequence recognition by karyopherin beta 2
    Brittany J Lee
    Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, 6001 Forest Park, Dallas, TX 75390, USA
    Cell 126:543-58. 2006
    ..These studies define and validate a new NLS that could not be predicted by primary sequence analysis alone...
  16. ncbi Nuclear export of microRNA precursors
    Elsebet Lund
    Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison, WI 53706, USA
    Science 303:95-8. 2004
    ..Thus, Exp5 is key to miRNA biogenesis and may help coordinate nuclear and cytoplasmic processing steps...
  17. ncbi Small interfering RNA-induced transcriptional gene silencing in human cells
    Kevin V Morris
    Department of Medicine 0678, Stein Clinical Research Building, Room 302, University of California San Diego, La Jolla, CA 92093 0678, USA
    Science 305:1289-92. 2004
    ..These results demonstrate that siRNA-directed transcriptional silencing is conserved in mammals, providing a means to inhibit mammalian gene function...
  18. ncbi Actin dynamics control SRF activity by regulation of its coactivator MAL
    Francesc Miralles
    Transcription Laboratory, Room 401, Cancer Research UK London Research Institute, Lincolns Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Cell 113:329-42. 2003
    ..Constitutively cytoplasmic MAL derivatives interfere with MAL redistribution and Rho-actin signaling to SRF. MAL associates with several SRF target promoters regulated via the Rho-actin pathway...
  19. ncbi Nuclear-cytoplasmic shuttling of APC regulates beta-catenin subcellular localization and turnover
    B R Henderson
    Westmead Institute for Cancer Research, University of Sydney, Westmead Millenium Institute, Westmead NSW 2145, Australia
    Nat Cell Biol 2:653-60. 2000
    ..These findings suggest that wild-type APC controls the nuclear accumulation of beta-catenin by a combination of nuclear export and cytoplasmic degradation...
  20. pmc Influenza virus targets the mRNA export machinery and the nuclear pore complex
    Neal Satterly
    Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9039, USA
    Proc Natl Acad Sci U S A 104:1853-8. 2007
    ..These findings demonstrate previously undescribed influenza-mediated viral-host interactions and provide insights into potential molecular therapies that may interfere with influenza infection...
  21. ncbi Estrogen-receptor-alpha exchange and chromatin dynamics are ligand- and domain-dependent
    Z Dave Sharp
    Molecular Medicine, University of Texas Instititue of Biotechnology, San Antonio, TX, USA
    J Cell Sci 119:4101-16. 2006
    ..Collectively, quantitative observations from this physiologically relevant biosensor suggest stochastic-based dynamics influence gene regulation at the promoter level...
  22. pmc Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes
    Theis Stüven
    ZMBH, INF 282, D 69120 Heidelberg and Universität Lübeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
    EMBO J 22:5928-40. 2003
    ..In contrast to a previous report, we found no indications of a major and direct role for CRM1 in actin export from mammalian or insect nuclei...
  23. pmc Using single-particle tracking to study nuclear trafficking of viral genes
    Hazen P Babcock
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Biophys J 87:2749-58. 2004
    ..We expect this single-particle tracking approach to find broad application in investigations of genetic trafficking...
  24. ncbi The carboxyl-terminal fragment of pro-HB-EGF reverses Bcl6-mediated gene repression
    Yumi Kinugasa
    Department of Biochemistry and Molecular Genetics, Ehime University Graduate School of Medicine, Shitsukawa, To on, Ehime 791 0295, Japan
    J Biol Chem 282:14797-806. 2007
    ..These findings suggest that HB-EGF-CTF is a potent regulator of gene expression via its interaction with the transcriptional repressor Bcl6...
  25. ncbi Classical nuclear localization signals: definition, function, and interaction with importin alpha
    Allison Lange
    Department of Biochemistry, School of Medicine, Emory University, Atlanta, Georgia 30322, USA
    J Biol Chem 282:5101-5. 2007
    ..Finally, we describe how a predicted NLS within a protein of interest can be confirmed experimentally to be functionally important...
  26. ncbi PDLIM2-mediated termination of transcription factor NF-kappaB activation by intranuclear sequestration and degradation of the p65 subunit
    Takashi Tanaka
    Laboratory for Host Defense, RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa 230 0045, Japan
    Nat Immunol 8:584-91. 2007
    ..Our findings delineate a pathway by which PDLIM2 terminates NF-kappaB activation through intranuclear sequestration and subsequent degradation...
  27. ncbi Control of the nuclear-cytoplasmic partitioning of annexin II by a nuclear export signal and by p11 binding
    D A Eberhard
    Department of Pharmacology, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    J Cell Sci 114:3155-66. 2001
    ..Thus, nuclear exclusion of AnxII results from nuclear export of the monomer and sequestration of AnxII/p11 complex, and may be modulated by phosphorylation...
  28. ncbi An auto-regulatory loop between stress sensors INrf2 and Nrf2 controls their cellular abundance
    Ok Hee Lee
    Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 282:36412-20. 2007
    ..The induction of INrf2 followed ubiquitination and degradation of Nrf2 and suppression of INrf2 gene expression. In conclusion, Nrf2 regulates INrf2 by controlling its transcription, and INrf2 controls Nrf2 by degrading it...
  29. pmc ER membrane-bending proteins are necessary for de novo nuclear pore formation
    T Renee Dawson
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Cell Biol 184:659-75. 2009
    ..We hypothesize that these ER membrane-bending proteins mediate early NPC assembly steps...
  30. ncbi Cyclin B1 and CDK1: nuclear localization and upstream regulators
    Lisa A Porter
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093 0367, USA
    Prog Cell Cycle Res 5:335-47. 2003
    ..Elucidating the role of mediators controlling cyclin B1-CDK1 translocation at the onset of mitosis is essential in developing drug targets for cell cycle control...
  31. ncbi The DECD box putative ATPase Sub2p is an early mRNA export factor
    T H Jensen
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, MA 02454, USA
    Curr Biol 11:1711-5. 2001
    ..Taken together, our data suggest that Sub2p functions at an early step in the mRNA export process...
  32. ncbi Apoptin nuclear accumulation is modulated by a CRM1-recognized nuclear export signal that is active in normal but not in tumor cells
    Ivan K H Poon
    Nuclear Signaling Laboratory, Department of Biochemistry and Molecular Biology, Monash University, Victoria, Australia
    Cancer Res 65:7059-64. 2005
    ..This unique combination of signals is the basis of the tumor cell-specific nuclear targeting abilities of VP3...
  33. pmc Structure-based design of a pathway-specific nuclear import inhibitor
    Ahmet E Cansizoglu
    Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, 6001 Forest Park, Dallas, Texas 75390 9041, USA
    Nat Struct Mol Biol 14:452-4. 2007
    ..On the basis of these data and complementary biochemical analyses, we designed a Kapbeta2-specific nuclear import inhibitor, M9M...
  34. ncbi Importin-mediated nuclear transport in neurons
    Klara Olofsdotter Otis
    University of California, Los Angeles, Gonda Research Building 3506C, 695 Charles Young Drive South, Los Angeles, CA 90095 1761, USA
    Curr Opin Neurobiol 16:329-35. 2006
    ..In neurons, importins might serve an additional function by carrying signals from distal sites to the soma...
  35. pmc Nuclear export of the APC tumour suppressor controls beta-catenin function in transcription
    Rina Rosin-Arbesfeld
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    EMBO J 22:1101-13. 2003
    ..Our evidence indicates that the rate of nuclear export of APC, rather than its nuclear import or steady-state levels, determines the transcriptional activity of beta-catenin...
  36. pmc Protein kinase A phosphorylation modulates transport of the polypyrimidine tract-binding protein
    Jiuyong Xie
    Howard Hughes Medical Institute, Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 100:8776-81. 2003
    ..Thus, direct PKA phosphorylation of PTB at Ser-16 modulates the nucleo-cytoplasmic distribution of PTB. This phosphorylation likely plays a role in the cytoplasmic function of PTB...
  37. pmc The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import
    B Fahrenkrog
    M E Müller Institute for Structural Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Mol Biol Cell 11:3885-96. 2000
    ..Whereas Nup53p is directly involved in NLS-mediated protein import by its interaction with the yeast nuclear import receptor Kap95p, it appears not to participate in NES-dependent nuclear export...
  38. pmc The mechanism of nuclear export of Smad3 involves exportin 4 and Ran
    Akira Kurisaki
    Ludwig Institute for Cancer Research, Box 595 Biomedical Center, SE 751 24 Uppsala, Sweden
    Mol Cell Biol 26:1318-32. 2006
    ..A short peptide representing the minimal interaction domain in Smad3 effectively competes with Smad3 association to exportin 4 and blocks nuclear export of Smad3 in vivo. We thus delineate a novel nuclear export pathway for Smad3...
  39. pmc Integrase interacts with nucleoporin NUP153 to mediate the nuclear import of human immunodeficiency virus type 1
    Cora L Woodward
    Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, California 90095, USA
    J Virol 83:6522-33. 2009
    ....
  40. ncbi Pre-ribosomes on the road from the nucleolus to the cytoplasm
    Herbert Tschochner
    Biochemie Zentrum Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Trends Cell Biol 13:255-63. 2003
    ..The future challenge will be to assign functions to the more than 150 non-ribosomal factors that transiently associate with the developing pre-ribosomes...
  41. ncbi AMP-activated protein kinase-regulated phosphorylation and acetylation of importin alpha1: involvement in the nuclear import of RNA-binding protein HuR
    Wengong Wang
    Laboratory of Cellular and Molecular Biology, NIA Intramural Research Program, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 279:48376-88. 2004
    ..Our results point to importin alpha1 as a critical downstream target of AMPK and key mediator of AMPK-triggered HuR nuclear import...
  42. pmc Importin beta-mediated nuclear import of fibroblast growth factor receptor: role in cell proliferation
    J F Reilly
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Cell Biol 152:1307-12. 2001
    ..These data are the first description of a nuclear import pathway for transmembrane growth factor receptors and elucidate a novel signal transduction pathway from the cell surface to the nucleus...
  43. ncbi Nitric oxide stimulates Nrf2 nuclear translocation in vascular endothelium
    Barbara J Buckley
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Biochem Biophys Res Commun 307:973-9. 2003
    ..These results suggest that ERK and p38 pathways may regulate nitric oxide-mediated adaptive responses in vascular endothelium via translocation of Nrf2 and activation of the ARE...
  44. ncbi LEDGF/p75 is essential for nuclear and chromosomal targeting of HIV-1 integrase in human cells
    Goedele Maertens
    Laboratory of Biomolecular Dynamics, Katholieke Universiteit Leuven, Celestijnenlaan 200D, B 3001 Leuven, Belgium
    J Biol Chem 278:33528-39. 2003
    ..We conclude, therefore, that the interaction with LEDGF/p75 accounts for the karyophilic properties and chromosomal targeting of HIV-1 IN...
  45. ncbi Pathways mediating the nuclear import of histones H3 and H4 in yeast
    Nima Mosammaparast
    Center for Cell Signaling, Department of Microbiology, University of Virginia Health Sciences Center, University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 277:862-8. 2002
    ..H3 and H4 are the first histones to be assembled onto DNA, and these results show that their import is mediated by at least two import pathways...
  46. pmc Activity-dependent nuclear translocation and intranuclear distribution of NFATc in adult skeletal muscle fibers
    Y Liu
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Cell Biol 155:27-39. 2001
    ..1-s trains at 50 Hz every 50 s. The stimulation pattern-dependent nuclear translocation of NFATc demonstrated here could thus contribute to fast-twitch to slow-twitch fiber type transformation...
  47. ncbi Docking of HIV-1 Vpr to the nuclear envelope is mediated by the interaction with the nucleoporin hCG1
    Erwann Le Rouzic
    Institut Cochin, Department of Infectious Diseases, CNRS UMR8104, INSERM U567, Universite Paris 5, Paris, France
    J Biol Chem 277:45091-8. 2002
    ..This association of Vpr with a component of the nuclear pore complex may contribute to the disruption of the nuclear envelope and to the nuclear import of the viral DNA...
  48. pmc STAT3 nuclear import is independent of tyrosine phosphorylation and mediated by importin-alpha3
    Ling Liu
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794 8691, USA
    Proc Natl Acad Sci U S A 102:8150-5. 2005
    ..These results distinguish STAT3 cellular localization from other STAT molecules and identify a feature that may be targeted in the clinical intervention of STAT3-dependent neoplasia...
  49. ncbi The synthetic triterpenoid 1-[2-cyano-3,12-dioxooleana-1,9(11)-dien-28-oyl]imidazole blocks nuclear factor-kappaB activation through direct inhibition of IkappaB kinase beta
    Mark M Yore
    Department of Pharmacology, Dartmouth Medical School, Hanover, NH 03755, USA
    Mol Cancer Ther 5:3232-9. 2006
    ..Furthermore, we show that Cys(179) on IKK is a target for CDDO-Im. This is the first report to show that this novel synthetic triterpenoid binds to and inhibits IKKbeta directly...
  50. ncbi Role of nucleoporin induction in releasing an mRNA nuclear export block
    Jost Enninga
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Science 295:1523-5. 2002
    ..Thus, increased Nup98 and Nup96 expression constitutes an IFN-mediated mechanism that reverses M protein-mediated inhibition of gene expression...
  51. ncbi Studying nuclear protein import in yeast
    Deena M Leslie
    Institute for Systems Biology, 1141 N 34th St, Seattle, WA 98103, USA
    Methods 39:291-308. 2006
    ....
  52. pmc Cellular stresses induce the nuclear accumulation of importin alpha and cause a conventional nuclear import block
    Yoichi Miyamoto
    Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamada oka, Suita, Osaka 565 0871, Japan
    J Cell Biol 165:617-23. 2004
    ..These findings suggest that the stress-induced nuclear accumulation of importin alpha can be involved in a common physiological response to various stress conditions...
  53. pmc Nuclear localization signal and protein context both mediate importin alpha specificity of nuclear import substrates
    Beate Friedrich
    The Max Delbrueck Center for Molecular Medicine, Robert Roessle Strasse 10, 13125 Berlin, Germany
    Mol Cell Biol 26:8697-709. 2006
    ..In vitro binding studies and nuclear import assays revealed that both NLS and protein context contribute to the specificity of importin alpha binding and transport...
  54. pmc Identification of the nuclear export signal and STAT-binding domains of the Nipah virus V protein reveals mechanisms underlying interferon evasion
    Jason J Rodriguez
    Immunobiology Center, The Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Virol 78:5358-67. 2004
    ..These findings characterize essential regions for Henipavirus V proteins that represent potential targets for therapeutic intervention...
  55. pmc Structural requirements for pre-microRNA binding and nuclear export by Exportin 5
    Yan Zeng
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Nucleic Acids Res 32:4776-85. 2004
    ..Moreover, these results argue that Exp5 binding not only mediates pre-microRNA nuclear export but also prevents nuclear pre-microRNA degradation...
  56. ncbi Intranuclear pre-mRNA trafficking in an insect model system
    Eva Kiesler
    Department of Molecular Biology and Functional Genomics, Stockholm University, 10961 Stockholm, Sweden
    Prog Mol Subcell Biol 35:99-118. 2004
  57. ncbi From birth to death: the complex lives of eukaryotic mRNAs
    Melissa J Moore
    Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, 415 South Street, Waltham, MA 02454
    Science 309:1514-8. 2005
    ..Thus, a comprehensive understanding of eukaryotic gene expression requires an appreciation for how the lives of mRNAs are influenced by a wide array of diverse regulatory mechanisms...
  58. pmc In situ fluorescence analysis demonstrates active siRNA exclusion from the nucleus by Exportin 5
    Thomas Ohrt
    Institute for Biophysics, BIOTEC, Dresden University of Technology, Tatzberg 47 51, 01307 Dresden, Germany
    Nucleic Acids Res 34:1369-80. 2006
    ..Exportin 5 therefore represents a key point of intersection between the siRNA and miRNA pathways, and, as such, is of fundamental importance for the design and interpretation of RNA interference experimentation...
  59. pmc SUMO modification of heterogeneous nuclear ribonucleoproteins
    Maria T Vassileva
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Mol Cell Biol 24:3623-32. 2004
    ..Based on our findings, we propose that SUMO modification of the hnRNP C and M proteins may occur at NPCs and facilitate the nucleocytoplasmic transport of mRNAs...
  60. ncbi Regulation of tumor suppressors by nuclear-cytoplasmic shuttling
    Megan Fabbro
    Westmead Institute for Cancer Research, University of Sydney, Westmead Millennium Institute at Westmead Hospital, New South Wales, Australia
    Exp Cell Res 282:59-69. 2003
    ..Here, we review the pathways by which tumor suppressors such as APC, p53, VHL, and BRCA1 cross the nuclear envelope and the impact of regulated nuclear import/export on protein function...
  61. ncbi Nuclear retention of unspliced mRNAs in yeast is mediated by perinuclear Mlp1
    Vincent Galy
    Unité de Biologie Cellulaire du Noyau, CNRS URA 2582, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Cell 116:63-73. 2004
    ..We propose that at this confined site the perinuclear Mlp1 implements a quality control step prior to export, physically retaining faulty pre-mRNAs...
  62. pmc Role and dynamics of the ribosomal protein P0 and its related trans-acting factor Mrt4 during ribosome assembly in Saccharomyces cerevisiae
    María Rodríguez-Mateos
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas and Universidad Autonoma de Madrid, Cantoblanco E 28049 Madrid, Spain
    Nucleic Acids Res 37:7519-32. 2009
    ..We conclude that Mrt4 controls the position and timing of P0 assembly. In turn, P0 is required for the release of Mrt4 and exchanges with this factor at the cytoplasm. Our results also suggest other P0 assembly alternatives...
  63. ncbi Design of peptide inhibitors for the importin alpha/beta nuclear import pathway by activity-based profiling
    Shunichi Kosugi
    Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan
    Chem Biol 15:940-9. 2008
    ..These peptide inhibitors provide a useful tool for studying nuclear import events. Moreover, our inhibitor design method should enable the development of potent inhibitors from a peptide seed...
  64. pmc The ins and outs of APC and beta-catenin nuclear transport
    Beric R Henderson
    Westmead Institute for Cancer Research, University of Sydney, Westmead Millennium Institute, NSW, Australia
    EMBO Rep 3:834-9. 2002
    ..Models that link APC and beta-catenin transport to function are discussed...
  65. pmc Nuclear epidermal growth factor receptor (EGFR) interacts with signal transducer and activator of transcription 5 (STAT5) in activating Aurora-A gene expression
    Liang Yi Hung
    Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan
    Nucleic Acids Res 36:4337-51. 2008
    ..The results also provide a novel linkage between the EGFR signaling pathway and overexpression of Aurora-A in tumorigenesis and chromosome instability...
  66. pmc Kinetic and molecular analysis of nuclear export factor CRM1 association with its cargo in vivo
    Dirk Daelemans
    Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium
    Mol Cell Biol 25:728-39. 2005
    ..CRM1 is able to bind Rev cargo in the nucleolus, and upon RanGTP binding a functional export complex is produced that is exported to the cytoplasm...
  67. pmc Importin alpha associates with membranes and participates in nuclear envelope assembly in vitro
    Virginie Hachet
    European Molecular Biology Laboratory, Meyerhofstrasse, Heidelberg, Germany
    EMBO J 23:1526-35. 2004
    ..Unlike other functions of Importin alpha, this membrane-associated activity does not require interaction with NLS proteins...
  68. pmc Function of dynein and dynactin in herpes simplex virus capsid transport
    Katinka Döhner
    Institute of Biochemistry, Hannover Medical School, Germany
    Mol Biol Cell 13:2795-809. 2002
    ..We concluded that incoming HSV1 capsids are propelled along MTs by dynein and that dynein and dynactin are required for efficient viral capsid transport to the nucleus...
  69. ncbi Regulation of Stat3 nuclear import by importin alpha5 and importin alpha7 via two different functional sequence elements
    Jing Ma
    Signal Transduction Laboratory, Institute of Molecular and Cell Biology, Proteos Building, 61 Biopolis Drive, Singapore, 138673, Republic of Singapore
    Cell Signal 18:1117-26. 2006
    ..A model for Stat3 nuclear translocation is proposed based on these data...
  70. pmc Novel nuclear import of Vpr promoted by importin alpha is crucial for human immunodeficiency virus type 1 replication in macrophages
    Yuko Nitahara-Kasahara
    Retrovirus Research Unit, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Virol 81:5284-93. 2007
    ..This study suggests the possibility that the binding of Vpr to importin alpha, preceding a novel nuclear import process, is a potential target for therapeutic intervention...
  71. ncbi Structural biology of nucleocytoplasmic transport
    Atlanta Cook
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Annu Rev Biochem 76:647-71. 2007
    ....
  72. ncbi Mutation of SAD2, an importin beta-domain protein in Arabidopsis, alters abscisic acid sensitivity
    Paul E Verslues
    Institute for Integrative Genome Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA
    Plant J 47:776-87. 2006
    ..The results suggest a role for nuclear transport in ABA signal transduction, and the possible roles of SAD2 in relation to that of SAD1 and ABH1 are discussed...
  73. pmc Recruitment of the complete hTREX complex is required for Kaposi's sarcoma-associated herpesvirus intronless mRNA nuclear export and virus replication
    James R Boyne
    Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, UK
    PLoS Pathog 4:e1000194. 2008
    ..Together, these data provide the first direct evidence that the complete hTREX complex is essential for the export of KSHV intronless mRNAs and infectious virus production...
  74. pmc Degradation of promoter-bound p65/RelA is essential for the prompt termination of the nuclear factor kappaB response
    Simona Saccani
    Institute for Research in Biomedicine, Via Vela 6, 6500 Bellinzona, Switzerland
    J Exp Med 200:107-13. 2004
    ..These results indicate that proteasomal degradation of p65/RelA does not merely regulate its stability and abundance, but also actively promotes transcriptional termination...
  75. ncbi Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex
    Samir S Patel
    MCD Biology, University of California Santa Cruz, Santa Cruz, CA 95064, USA
    Cell 129:83-96. 2007
    ..The results support a two-gate model of NPC architecture featuring a central diffusion gate formed by a meshwork of cohesive FG nucleoporin filaments and a peripheral gate formed by repulsive FG nucleoporin filaments...
  76. ncbi A high-efficiency protein transduction system demonstrating the role of PKA in long-lasting long-term potentiation
    M Matsushita
    First Department of Physiology, Okayama University Medical School, Okayama 700-8558, Japan
    J Neurosci 21:6000-7. 2001
    ..These results highlight transduction of proteins and peptides into specific neuronal subcellular compartments in brain slices as a powerful tool for studying neuronal plasticity...
  77. ncbi Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport
    Hidetaka Kosako
    Division of Cellular Proteomics BML, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    Nat Struct Mol Biol 16:1026-35. 2009
    ..Thus, we propose that ERK phosphorylates various nucleoporins to regulate nucleocytoplasmic transport...
  78. ncbi Wg/Wnt signal can be transmitted through arrow/LRP5,6 and Axin independently of Zw3/Gsk3beta activity
    Nicholas S Tolwinski
    Howard Hughes Medical Institute, Princeton University, Princeton, NJ 08544, USA
    Dev Cell 4:407-18. 2003
    ..We propose that Arrow (LRP) recruits Axin to the membrane, and that this interaction leads to Axin degradation. As a consequence, Armadillo is no longer bound by Axin, resulting in nuclear signaling by Armadillo...
  79. ncbi Myb-binding protein 1a is a nucleocytoplasmic shuttling protein that utilizes CRM1-dependent and independent nuclear export pathways
    Rebecca A Keough
    Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide, South Australia 5000, Australia
    Exp Cell Res 289:108-23. 2003
    ..Deletion analysis and in vivo export studies using a heterologous assay system identified several nuclear export sequences which facilitate Mybbp1a nuclear export of Mybbp1a by CRM1-dependent and -independent pathways...
  80. ncbi Nuclear export of glucocorticoid receptor is enhanced by c-Jun N-terminal kinase-mediated phosphorylation
    M Itoh
    First Department of Internal Medicine, Sapporo Medical University School of Medicine, Sapporo, 060 8543, Japan
    Mol Endocrinol 16:2382-92. 2002
    ..Taken together, these findings suggest that JNK-mediated phosphorylation of the GR-Ser226 enhances GR nuclear export and may contribute to termination of GR-mediated transcription...
  81. ncbi Nuclear-cytoplasmic transport of EGFR involves receptor endocytosis, importin beta1 and CRM1
    Hui Wen Lo
    Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Blvd, Houston, Texas 77030, USA
    J Cell Biochem 98:1570-83. 2006
    ....
  82. ncbi Ratjadone and leptomycin B block CRM1-dependent nuclear export by identical mechanisms
    Torsten Meissner
    Abteilung Zellulare Signalverarbeitung, Leibniz Forschungsinstitut fur Molekulare Pharmakologie, Freie Universitat Berlin, Robert Rossle Str 10, 13125 Berlin, Germany
    FEBS Lett 576:27-30. 2004
    ..Here, we show that LMB and RAT A employ the same molecular mechanism to inactivate CRM1. Alkylation of residue Cys528 of CRM1 determines both LMB and RAT sensitivity and prevents nuclear export of CRM1 cargo proteins...
  83. ncbi Nrf2 controls constitutive and inducible expression of ARE-driven genes through a dynamic pathway involving nucleocytoplasmic shuttling by Keap1
    Truyen Nguyen
    Schering Plough Research Institute, Kenilworth, New Jersey 07033, USA
    J Biol Chem 280:32485-92. 2005
    ....
  84. pmc Nuclear export and retention signals in the RS domain of SR proteins
    Demian Cazalla
    MRC Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, Scotland
    Mol Cell Biol 22:6871-82. 2002
    ..However, the SR protein SC35 has two long stretches of RS repeats, yet it is not a shuttling protein. We demonstrate the presence of a dominant nuclear retention signal in the RS domain of SC35...
  85. pmc Glycogen synthase kinase-3 enhances nuclear export of a Dictyostelium STAT protein
    R S Ginger
    Department of Anatomy and Physiology, MSI WTB complex, University of Dundee, Dow Street, Dundee, UK
    EMBO J 19:5483-91. 2000
    ..It also raises the possibility of an analogous regulation of STAT nuclear export in higher eukaryotes...
  86. pmc Evidence for a functional link between uncoating of the human immunodeficiency virus type 1 core and nuclear import of the viral preintegration complex
    David J Dismuke
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, A 5301 Medical Center North, Nashville, Tennessee 37232 2363, USA
    J Virol 80:3712-20. 2006
    ..These results demonstrate that mutations in CA can have deleterious effects on both nuclear targeting and integration, suggesting that these steps in the HIV-1 life cycle are dependent on proper uncoating of the viral core...
  87. pmc Integrin-mediated adhesion regulates ERK nuclear translocation and phosphorylation of Elk-1
    A E Aplin
    Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Cell Biol 153:273-82. 2001
    ..These data show that integrin-mediated organization of the actin cytoskeleton regulates localization of activated ERK, and in turn the ability of ERK to efficiently phosphorylate nuclear substrates...
  88. pmc Enteropathogenic Escherichia coli (EPEC) inactivate innate immune responses prior to compromising epithelial barrier function
    Marie Hélène Ruchaud-Sparagano
    Institute for Cell and Molecular Biosciences, Medical School, University of Newcastle, Framlington Place, Newcastle upon Tyne, NE2 4HH, UK
    Cell Microbiol 9:1909-21. 2007
    ..Thus, these findings also lend themselves to a plausible mechanism to explain the absence of a strong inflammatory response in EPEC-infected humans...
  89. ncbi Nuclear import and export signals in control of Nrf2
    Abhinav K Jain
    Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030, USA
    J Biol Chem 280:29158-68. 2005
    ..Antioxidants change this balance in favor of nuclear accumulation of Nrf2, leading to activation of chemopreventive proteins. Once this is achieved, Nrf2 exits the nucleus for binding to INrf2 and degradation...
  90. pmc Exportin-5, a novel karyopherin, mediates nuclear export of double-stranded RNA binding proteins
    Amy M Brownawell
    Center for Cell Signaling, University of Virginia, Charlottesville, VA 22908, USA
    J Cell Biol 156:53-64. 2002
    ..We propose that exportin-5 is not an RNA export factor but instead participates in the regulated translocation of dsRBD proteins to the cytoplasm where they interact with target mRNAs...
  91. ncbi Nuclear exclusion of Smad2 is a mechanism leading to loss of competence
    Oliver H Grimm
    Wellcome Trust Cancer Research UK Institute, Tennis Court Road, University of Cambridge, Cambridge, CB2 1QR, UK
    Nat Cell Biol 4:519-22. 2002
    ..This mechanism operates within less than an hour, and is therefore well suited to control an orderly sequence of inductions...
  92. pmc Replacement of the yeast TRP4 3' untranslated region by a hammerhead ribozyme results in a stable and efficiently exported mRNA that lacks a poly(A) tail
    Katrin Düvel
    Institute of Microbiology and Genetics, Georg August University, Gottingen, Germany
    RNA 8:336-44. 2002
    ..This translational defect allows sufficient synthesis of Trp4p to support growth of trp4 cells, but is, nevertheless, of such magnitude as to activate the general control network of amino acid biosynthesis...
  93. ncbi Characterization of the nuclear import and export functions of Ikappa B(epsilon)
    Sang Hyun Lee
    Biochemistry Department University of Missouri, Columbia, Missouri 65212, USA
    J Biol Chem 277:23358-66. 2002
    ....
  94. ncbi Minihelix-containing RNAs mediate exportin-5-dependent nuclear export of the double-stranded RNA-binding protein ILF3
    Carole Gwizdek
    Institut Jacques Monod, Unite Mixte de Recherche 7592, CNRS, Universités Paris VI et VII, 2 place Jussieu, Tour 43, Paris 75251 Cedex 05, France
    J Biol Chem 279:884-91. 2004
    ..Exportin-5 thus appears as the first example of a nuclear export receptor that mediates RNA export but also promotes transport of proteinaceous cargo through appropriate and specific RNA adaptors...
  95. pmc A PY-NLS nuclear targeting signal is required for nuclear localization and function of the Saccharomyces cerevisiae mRNA-binding protein Hrp1
    Allison Lange
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 283:12926-34. 2008
    ..Finally, we apply this analysis to a bioinformatic search of the yeast proteome as a preliminary search for new potential Kap104 cargos...
  96. ncbi Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor
    Michael E Nemergut
    Department of Microbiology, The Center for Cell Signaling, The University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 277:17385-8. 2002
    ..By tethering Crm1 to catalytically enhanced RCC1, RanBP3 may lower the entropic barrier for the loading of Ran.GTP onto Crm1. We propose that this provides an additional mechanism by which RanBP3 facilitates export...
  97. pmc Multiple nuclear localization signals function in the nuclear import of the transcription factor Nrf2
    Melanie Theodore
    School of Medicine, Meharry Medical College, Nashville, TN 37208 3599, USA
    J Biol Chem 283:8984-94. 2008
    ....
  98. ncbi Import of adenovirus DNA involves the nuclear pore complex receptor CAN/Nup214 and histone H1
    L C Trotman
    , Institute of Zoology, Winterthurerstrasse 190, , Switzerland
    Nat Cell Biol 3:1092-100. 2001
    ..Our results provide a molecular mechanism for disassembly of Ad2 and reveal an unexpected function of histone H1 in virus-mediated DNA import...
  99. pmc Yvh1 is required for a late maturation step in the 60S biogenesis pathway
    Stefan Kemmler
    Institute of Biochemistry, ETH Zurich, CH 8093 Zurich, Switzerland
    J Cell Biol 186:863-80. 2009
    ..Together, our data suggest that Yvh1-mediated release of Mrt4 precedes cytoplasmic loading of Rpp0 on pre-60S particles and is an obligatory late step toward construction of translation-competent 60S subunits...
  100. ncbi Vesicular stomatitis virus matrix protein inhibits host cell gene expression by targeting the nucleoporin Nup98
    - von Kobbe C
    European Molecular Biology Laboratory Meyerhofstrasse 1 D 69117, Heidelberg, Germany
    Mol Cell 6:1243-52. 2000
    ..Together, our data demonstrate that VSV M inhibits host cell gene expression by targeting a nucleoporin and primarily blocking nuclear export...
  101. pmc Importin-beta-like nuclear transport receptors
    A C Ström
    Department of Molecular and Cellular Biology, University of California, Berkeley, CA 94720 3200, USA
    Genome Biol 2:REVIEWS3008. 2001
    ..Thus, common features of all importin-beta-like transport factors are their ability to shuttle between the nucleus and the cytoplasm, their interaction with RanGTP as well as their ability to recognize specific transport substrates...

Research Grants32

  1. HIV-1 TROPISM AND CHEMOKINE RECEPTORS
    BRYAN CULLEN; Fiscal Year: 2002
    ..The final specific aim focusses on the promoter regions of CCR5, the identification of transcription factors that may interact with the promoter, and the use of antisense strategies to block CCR5 expression. ..
  2. Nucleocytoplasmic Trafficking of Smads
    Lan Xu; Fiscal Year: 2008
    ..These regulators of subcellular localization potential targets for therapeutic interventions aimed at modulating TGF-beta signaling diseases such as cancer. ..
  3. Regulation of human microRNA biosynthesis and activity
    BRYAN CULLEN; Fiscal Year: 2007
    ..Finally, in specific aim 5, we seek to extend our exciting initial data demonstrating inhibition of miRNA function by the adenovirus VA1 non-coding RNA by trying to define the underlying mechanism. ..
  4. Effect of APOBEC3G on retroviruses and retrotransposons
    BRYAN CULLEN; Fiscal Year: 2009
    ..abstract_text> ..
  5. Molecular Mechanism of HIV-1 Vif Function
    BRYAN CULLEN; Fiscal Year: 2007
    ..Together, this wide-ranging series of experiments should lead to considerable insight into how HIV-1 Vif functions and potentially suggest approaches to inhibit Vif function. ..
  6. DUX4 and Facioscapulohumeral Muscular Dystrophy
    Howard Worman; Fiscal Year: 2004
    ..This work could lead to the development of new diagnostic methods as well as the identification of potential protein targets for the treatment of FSHD. ..
  7. High Through-Put Genomics Approach To Lifespan in Yeast
    David Goldfarb; Fiscal Year: 2006
    ..The resulting public data set is likely to identify new aging genes that could be of considerable importance to human aging. ..
  8. Lamin A Mutation and Hutchinson-Gilford Progeria
    Howard Worman; Fiscal Year: 2008
    ..This project will establish how mutations in nuclear lamins A and C cause HGPS, and if inhibition of protein farnesylation is a potential therapeutic intervention. ..
  9. Study of novel REST/NRSF-Interacting LIM domain Protein
    Masahito Shimojo; Fiscal Year: 2005
    ..Dr. Shimojo will have access to shared-use equipment, laboratory space, and faculty and staff, which will enhance the likelihood that the proposed objectives will be successfully accomplished. ..
  10. GLOMERULAR LAMININS: STRUCTURE AND FUNCTION
    Christine Abrass; Fiscal Year: 2003
    ....
  11. Molecular Mechanisms of Mesangial Sclerosis
    Christine Abrass; Fiscal Year: 2009
    ..Understanding transcriptional control of LN isoform expression will add new insights into the role that LN plays in normal glomerulogenesis and that becomes disordered in glomerular disease. ..
  12. Transplantation of Reduced-Size Fatty Livers
    Zhi Zhong; Fiscal Year: 2010
    ....
  13. The effects of strain on vocal fold fibroblasts
    Ryan Branski; Fiscal Year: 2004
    ..Furthermore, it seeks to provide in vitro data in conjunction with the Sponsor's current NIH ROI. ..
  14. Lamin A in Adipocyte Differentation and Survival
    Howard Worman; Fiscal Year: 2004
    ..Confirmation of this hypothesis will have implications for the identification of new cellular targets to treat human obesity and diabetes mellitus. ..
  15. Histone Acetylation and Insulin Gene Expression
    Sabire Ozcan; Fiscal Year: 2010
    ..In addition, the data obtained will contribute to our understanding of Pdx-1, MafA and NeuroD1 function in the regulation of pancreas development and glucose-stimulated insulin gene transcription. ..
  16. Mechanisms of In Vivo Ethanol-Induced Mitochondrial Depolarization
    Zhi Zhong; Fiscal Year: 2010
    ....
  17. Nuclear functions of the tumor suppressor protein APC
    KRISTI NEUFELD; Fiscal Year: 2008
    ..Greater knowledge of APC function in normal cells will improve our understanding of APC's role in tumorigenesis and ultimately illuminate new points for therapeutic intervention. ..
  18. Inhibition of colon tumor progression by Ink4a/Arf
    Gregory Enders; Fiscal Year: 2007
    ....
  19. Enteric Neuronal Survival in Diabetes
    Shanthi Srinivasan; Fiscal Year: 2008
    ....
  20. Advances in Mineral Metabolism (AIMM) annual meeting
    Rene St Arnaud; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  21. Formation of Heterochromatin and Cellular Senescence Driven by HIRA and ASF 1a
    Gregory Enders; Fiscal Year: 2009
    ..Specific Aim 3. Investigate the role of GSK3 activity in localization of HIRA to PML bodies, formation of SAHF and onset of senescence. ..
  22. G2 Role For Cdk2 in Human Cells
    Gregory Enders; Fiscal Year: 2007
    ..We believe that deregulation of Cdk2, a frequent event in cancer, may contribute to genetic instability and neoplastic progression through deregulation of these functions. ..
  23. ACUTE MYOCARDITIS--ROLES OF REOVIRUS AND INTERFERON BETA
    Barbara Sherry; Fiscal Year: 2001
    ..Reoviruses offer a unique tool to investigate the IFN response invoked by closely related viruses in the heart. Our work will be the first to identity cell factors that are determinants of this cardiac IFN response and myocarditis. ..
  24. Cell Type-Specific Viral Translation in the CNS
    Matthias Gromeier; Fiscal Year: 2006
    ..This principle has shown great promise in pre-clinical studies in experimental animals and in primary explant cultures of human CNS tumors. ..
  25. Tat Interaction with LRP and Its Role in AIDS Dementia
    Johnny He; Fiscal Year: 2006
    ..In addition, these studies will also yield new clues for developing anti-HIV therapeutic strategies for treating HIV-associated neurological disorders occurring in the majority of AIDS patients. ..
  26. Transcriptional Regulation of a Surfactant Enzyme
    RAMA MALLAMPALLI; Fiscal Year: 2005
    ..Ultimately, the results from these studies could lead to therapeutic strategies directed at modulating expression of this key surfactant enzyme at the molecular level. ..
  27. Poxvirus adverse effects on cardiac cells and the heart
    Barbara Sherry; Fiscal Year: 2005
    ..Studies proposed in this R21 application will provide the foundation for future in-depth studies with the goal of improving current and future smallpox vaccines. ..
  28. EBOLA VIRUS INTERFERON-ANTAGONISTS AND VIRULENCE
    CHRISTOPHER BASLER; Fiscal Year: 2003
    ..VP35 and VP24 may also prove to be attractive targets for new antiviral agents. Inhibition of anti-IFN factors may sensitize Ebola virus infected cells to the antiviral effects of IFN and may also enhance the effect of antiviral agents. ..
  29. Activation of a DNA damage pathway by HIV-1 vpr
    Vicente Planelles; Fiscal Year: 2003
    ..The goal of this proposal is to elucidate the specific steps of the DNA-response pathway that are activated by Vpr, and to compare such steps with the physiological activator, DNA damage. ..
  30. 2005 Bones & Teeth Gordon Conference
    Rene St Arnaud; Fiscal Year: 2005
    ..These funds, if granted, will aid immeasurably in allowing the Gordon Research Conference to fulfill its function to stimulate further creativity in bone, cartilage and tooth research. ..
  31. Advances in Mineral Metabolism (AMM) meeting
    Rene St Arnaud; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  32. Cytokine signal transduction in immune system
    Hodaka Fujii; Fiscal Year: 2007
    ..Detailed information gained from the proposed experiments will be valuable for elucidation of molecular mechanisms of oncogenesis and autoimmune diseases caused by dysregulation of cytokine signaling. ..