- Application of impedance spectroscopy for monitoring colloid Au-enhanced antibody immobilization and antibody-antigen reactions
College of Chemistry, Jilin University, Changchun 130021, PR China
Biosens Bioelectron 19:575-82. 2004
..The results show that this method has good correlation for detection of Hepatitis B virus surface antigen in the range of 0.5-200 microg/l and a detection limit of about 50 ng/l...
- Human monoclonal antibody against Hepatitis B virus surface antigen (HBsAg)
Yong Won Shin
Antibody Engineering Laboratory, Research Center, Green Cross Corporation, 341 Bojeong dong, Giheung gu, Yongin City, Gyunggi do 446 799, Republic of Korea
Antiviral Res 75:113-20. 2007
..The HB-C7A binds to HBV-infected human liver tissue but does not bind to normal human tissues. This HB-C7A has several advantages compared to plasma-derived Hepabig such as activity, safety and availability...
- Protease-sensitive scrapie prion protein in aggregates of heterogeneous sizes
Department of Molecular Biology, The Hebrew University Hadassah Medical School, and Hadassah University Hospital, Jerusalem 91120, Israel
Biochemistry 41:12868-75. 2002
..Whether these new PrP(Sc) species play a role in the biogenesis or the pathogenesis of prions remains to be established...
- Rituximab (monoclonal anti-CD20 antibody): mechanisms of action and resistance
Mitchell R Smith
Lymphoma Service, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA
Oncogene 22:7359-68. 2003
..As the first of an expanding class of anticancer agents, lessons learned regarding the mechanism of rituximab action and resistance will be of increasing importance...
- Genetic, structural and functional properties of an IgG DNA-binding monoclonal antibody from a lupus patient with nephritis
C T Ravirajan
Department of Medicine, University College London, GB
Eur J Immunol 28:339-50. 1998
..In this study we have shown for the first time using electron microscopy that a human IgG anti-dsDNA mAb, RH-14, is nephritogenic and that deposition of such an antibody alone is sufficient to induce renal damage...
- Mechanisms of transfusion-related acute lung injury (TRALI): anti-leukocyte antibodies
Brian R Curtis
Platelet and Neutrophil Immunology Laboratory, BloodCenter of Wisconsin, Milwaukee, WI, USA
Crit Care Med 34:S118-23. 2006
..Genotyping for class I and II HLA and for a limited number of neutrophil antigens may also be helpful in establishing antibody-antigen concordance...
- Biosensor immunosurface engineering inspired by B-cell membrane-bound antibodies: modeling and analysis of multivalent antigen capture by immobilized antibodies
Department of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
IEEE Trans Nanobioscience 2:14-25. 2003
..An analysis of the derived model provides insight into the design of biosensor immunosurfaces to enhance pathogen capture capability...
- Isolation of human anti-idiotypes broadly cross reactive with anti-dsDNA antibodies from patients with Systemic lupus erythematosus
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma University Health Sciences Center, Oklahoma City, OK 73104, USA
Scand J Immunol 53:192-7. 2001
..Such anti-idiotypic antibodies could play a significant role in the regulation of anti-dsDNA antibody levels in SLE...
- [Specific and nonspecific interactions of antibodies and immunoglobulins with antigens and the methods of analysis of these interactions]
S A Bobrovnik
Ukr Biokhim Zh 76:132-9. 2004
..The methods of the diminishing the non-specific interaction are suggested...
- Differential interactions of phytochrome A (Pr vs. Pfr) with monoclonal antibodies probed by a surface plasmon resonance technique
Hitachi Advanced Research Laboratory, Hatoyama, Saitama 350 0395, Japan
Photochem Photobiol Sci 6:83-9. 2007
- Outer membrane protein-specific monoclonal antibodies protect SCID mice from fatal infection by the obligate intracellular bacterial pathogen Ehrlichia chaffeensis
J S Li
Department of Biomedical Sciences, School of Public Health, State University of New York, Albany, NY 12201, USA
J Immunol 166:1855-62. 2001
- Immunoglobulin M efficacy against Cryptococcus neoformans: mechanism, dose dependence, and prozone-like effects in passive protection experiments
C P Taborda
Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
J Immunol 166:2100-7. 2001
- Investigating specific antigen/antibody binding with the atomic force microscope
Laboratoire de Physique des Interfaces, Faculté des Science de Monastir, Monastir, Tunisia
Biomol Eng 19:183-8. 2002
..The analysis using the height histograms reveals many interesting features. We propose a general framework for interpreting these analysis, which enables the discrimination between specific and non-specific complexes...
- Grafting of "abbreviated" complementarity-determining regions containing specificity-determining residues essential for ligand contact to engineer a less immunogenic humanized monoclonal antibody
Roberto De Pascalis
Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 169:3076-84. 2002
..The final variant of the HuCOL-1, which retains its Ag-binding reactivity and shows significantly lower serum reactivity than that of the parental Ab, can serve as a prototype for the development of a potentially useful clinical reagent...
- Structural basis for recognition of CD20 by therapeutic antibody Rituximab
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
J Biol Chem 282:15073-80. 2007
..These results provide a molecular basis for the specific recognition of CD20 by Rituximab as well as valuable information for development of improved antibody drugs with better specificity and higher affinity...
- Detection of 2,4,6-trinitrotoluene in seawater using a reversed-displacement immunosensor
Tiffanee M Green
Center for Bio Molecular Science and Engineering, US Naval Research Laboratory, CBMSE Code 6900, 4555 Overlook Ave SW, Washington, DC 20375 5320, USA
Anal Biochem 310:36-41. 2002
- Production of monoclonal antibodies for detection of a secreted aspartyl proteinase from Candida spp. in biologic specimens
Janaina Aparecida De Oliveira Rodrigues
Area de Microbiologia e Imunologia, Departamento de Diagnóstico Oral, Faculdade de Odontologia de Piracicaba, UNICAMP, SP, Brazil
Hybridoma (Larchmt) 26:201-10. 2007
..albicans and C. dubliniensis extracts. The results herein obtained allow for the prediction of how this set of antibodies can be useful for SAP detection in biologic specimens...
- Antibody nanoarrays with a pitch of approximately 20 nanometers
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA
J Am Chem Soc 128:12664-5. 2006
..In the resulting tetragonal antibody arrays, each spot is a similarly orientated, individual antibody molecule (IgG). The separation between two adjacent IgG molecules is only approximately 20 nm...
- Stereoselective recognition of monolayers of cholesterol, ent-cholesterol, and epicholesterol by an antibody
Department of Structural Biology, Weizmann Institute of Science, Rehovot, 76100 Israel
Chembiochem 2:265-71. 2001
..The lack of chiral discrimination by the antibody is thus correlated to the level at which the chirality is exposed at the surface of the monolayers...
- Hemagglutinin linear epitope presentation on monolayer-protected clusters elicits strong antibody binding
Aren E Gerdon
Department of Chemistry, Vanderbilt University, VU Station B, Nashville, Tennessee 37235 1822, USA
Biomacromolecules 6:3419-24. 2005
..Water-soluble nanoparticles that present known neutralizing peptide epitopes of protein antigens might be used in antiviral influenza vaccines...
- Specificity of antibodies: unexpected cross-reactivity of antibodies directed against the excitatory amino acid transporter 3 (EAAT3)
Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, P O Box 1105, Blindern, N 0317 Oslo, Norway
Neuroscience 136:649-60. 2005
..The specificity of an antibody is hard to predict because cross-reactivity can be specific and to unrelated molecules. The antigen preabsorption test is of little value in testing the specificity of affinity purified antibodies...
- Epitope mapping of the protective antigen of B. anthracis by using nanoclusters presenting conformational peptide epitopes
Aren E Gerdon
Department of Chemistry, Vanderbilt University, Station B 351822, Nashville, TN 37235, USA
Angew Chem Int Ed Engl 45:594-8. 2006
- [Dissection of specificity and affinity of an antibody for its specific antigen]
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Japan
Seikagaku 78:93-109. 2006
- Characterization of an anti-MUC1 monoclonal antibody with potential as a cancer vaccine
AltaRex Corp, Edmonton, AB, Canada
Hybrid Hybridomics 20:313-24. 2001
..5, but not in mice treated with control murine antibody or PBS (p < 0.001 and p < 0.05, respectively). An anti-tumor effect could also be demonstrated in a CB6F1 mouse model with the MUC1 transfectoma 413BCR...
- Oxidation of beta2-glycoprotein I (beta2GPI) by the hydroxyl radical alters phospholipid binding and modulates recognition by anti-beta2GPI autoantibodies
Laboratoire d Immunologie, Institut de Synergie des Sciences et de la Sante, CHU Brest, France
Thromb Haemost 86:1070-6. 2001
..These findings may be of clinical relevance for the generation and/or reactivity of anti-beta2GPI antibodies...
- A novel tumor-associated mucin of gastrointestinal carcinoma
K M Hong
Department of Biochemistry, Wonkwang University College of Medicine, Iksan, Korea
J Cancer Res Clin Oncol 127:551-8. 2001
..Therefore, SC142 may become a valuable tool for the immunohistochemical diagnosis and tumor immunoscintigraphy of the gastrointestinal cancer patients...
- Design of immunogens that present the crown of the HIV-1 V3 loop in a conformation competent to generate 447-52D-like antibodies
Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India
Biochem J 399:483-91. 2006
..The data suggest that because of the low accessibility of the V3 loop on primary isolates such as JRFL, it will be difficult to elicit a V3-specific, 447-52D-like antibody response to effectively neutralize such isolates...
- Analysis of the binding of bispecific monoclonal antibodies with immobilized antigens (human IgG and horseradish peroxidase) using a resonant mirror biosensor
Dmitriy A Dmitriev
Division of Chemical Enzymology, Department of Chemistry, Moscow State University, Moscow, Russian Federation
J Immunol Methods 261:103-18. 2002
..On the other hand, these data demonstrate that real-time analysis of antibody binding parameters in IAsys biosensor is valuable for the selection of mAbs and bAbs with desired features, for different fields of application...
- Generation of a humanised single chain Fv (Scfv) derived from the monoclonal Eric-1 recognising the human neural cell adhesion molecule
H A Whittington
Division of Oncology, University of Bristol, United Kingdom
Med Pediatr Oncol 36:243-6. 2001
..CONCLUSIONS: The humanised scFv may prove to be a useful reagents in the treatment and diagnosis of a variety of neuroectodermal tumours and can clearly form a suitable template for the generation of a fully humanised ERIC-1 MoAb...
- Section 1A: Rh serology. Coordinator's report
International Blood Group Reference Laboratory, Southmead Road, Bristol, BS10 5ND, UK
Transfus Clin Biol 9:23-9. 2002
..No patterns of reactivity corresponding to the epitope groups could be observed with the different types of weak D tested. Anti-E Mabs were sorted into 14 groups, and the E variant cells into seven groups...
- Mutations of an epitope hot-spot residue alter rate limiting steps of antigen-antibody protein-protein associations
Basic Research Laboratory, Program in Structural Biology, National Cancer Institute, Frederick Cancer Research and Development Center, P.O. Box B, Building 469, Room 206, Frederick, Maryland 21702-1201, USA
Biochemistry 40:2011-22. 2001
..Our results are consistent with the interpretation that the free energy barriers to conformational rearrangement are highest in H26, especially with mutant antigen...
- Production of anti-digoxigenin antibody HRP conjugate for PCR-ELISA DIG detection system
Department of Medical Biotechnology, Tarbiat Modarres University, Tehran, Iran
J Immunoassay Immunochem 27:303-18. 2006
..Several biochemical protocols and modifications were applied to increase the sensitivity and specificity of this conjugate for an efficient and cost-effective product...
- Preparation and characterization of anti-anti-idiotypic monoclonal antibody ("Ab1-like Ab3") in relation to carcinoembryonic antigen
Department of Internal Medicine Section 1, Sapporo Medical University, School of Medicine, Sapporo, Japan
J Clin Lab Anal 16:279-89. 2002
..These results indicate that anti-anti-Id MoAb 11B2 is "Ab1-like Ab3." Therefore, it is suggested that anti-Id MoAb M315 bears an "internal image" of the MoAb P1-356-defined epitope on CEA...
- Combinatorial experimental protocols for Erbicin-derived immunoagents and Herceptin
C De Lorenzo
Dipartimento di Biologia Strutturale e Funzionale, Universita di Napoli Federico II, Via Cinthia, Napoli 80126, Italy
Br J Cancer 97:1354-60. 2007
- Identification of the epitope of a monoclonal antibody to DJ-1
David W Miller
Laboratory of Neurogenetics, National Institute on Aging, Bldg 35, Rm 1A 1002, 35 Convent Drive, Bethesda, MD 20892, USA
Neurosci Lett 374:203-6. 2005
..Moreover, the loss of immunoreactivity due to such a small substitution demonstrates the remarkable sensitivity of the monoclonal antibody 3E8 to DJ-1...
- Expression and functional activity of isotype and subclass switched human monoclonal antibody reactive with the base of the V3 loop of HIV-1 gp120
Beth Israel Deaconess Medical Center, Massachusetts 02215, USA
AIDS Res Hum Retroviruses 19:597-607. 2003
- Interaction between the antigen and antibody is controlled by the constant domains: normal mode dynamics of the HEL-HyHEL-10 complex
School of Pharmaceutical Sciences, Kitasato University, 5 9 1 Shirokane, Minato ku, Tokyo 108 8641, Japan
Protein Sci 12:2125-31. 2003
..UL2-CL could play an important role and could be attractive for modification in protein engineering...
- Serological studies of monoclonal RH antibodies with RH1 (D), RH2 (C), RH3 (E) and RH5 (e) variant RBCs
Etablissement Français du Sang, Site Henri Mondor, 56, avenue du Maréchal de Lattre de Tasssigny, 94010 Creteil, France
Transfus Clin Biol 10:319-23. 2003
..Therefore, we drew parallels between our procedures and results obtained with the 2001 workshop antibodies...
- Ultrastructural analysis of the dynactin complex: an actin-related protein is a component of a filament that resembles F-actin
D A Schafer
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218
J Cell Biol 126:403-12. 1994
- Studies on experimental iodine allergy: 1. Antigen recognition of guinea pig anti-iodine antibody
Department of Drug Safety Research, Eisai Kawashima Research Laboratories, Eisai Co, Ltd, 1 Takehaya cho, Kawashima cho, Hashima gun, Gifu 501 0061, Japan
J Toxicol Sci 29:131-6. 2004
- Screening for epitope specificity directly on culture supernatants in the early phase of monoclonal antibody production by an ELISA with biotin-labeled antigen
Ditte C Andersen
Department of Immunology and Microbiology, University of Southern Denmark, Odense C, Denmark
J Immunoassay Immunochem 25:147-57. 2004
..Moreover, the bonus effect, i.e., a signal in excess of the reference signal when sets of monoclonal antibodies with different epitope specificity are compared, gives a relative measure of affinity...
- A new approach for fluorescence correlation spectroscopy (FCS) based immunoassays
Oleg A Mayboroda
Department of Parasitology, Leiden University Medical Centre LUMC, The Netherlands
J Biotechnol 107:185-92. 2004
..We have evaluated the feasibility of this approach in a model system consisting of mouse monoclonal IgG directed against the Lewis X antigen, and Protein A as a low molecular weight tag...
- Studies with Synthetic Peptides of 80 kDa Human Sperm Antigen (80 kDa HSA)
Vandana J Vernekar
National Institute for Research, in Reproductive Health, J M Street, Parel, Mumbai, India
Am J Reprod Immunol 51:106-11. 2004
..These sequenced peptides were synthesized and conjugated to key hole limpet haemocyanin (KLH) and evaluated for its antifertility effects. The present communication describes the characterization of these peptides and their antibodies...
- Characterization of a single-chain intrabody directed against the human receptor tyrosine kinase Ron
Department of Medical Sciences and Interdisciplinary Research Center of Autoimmune Diseases IRCAD, University of Eastern Piedmont A Avogadro, Via Solaroli 17, 28100 Novara, Italy
J Immunol Methods 285:99-109. 2004
..We demonstrate that the isolated antibody fragment interacts in vivo with the intracellular domain of Ron in mammalian cells...
- Polyreactivity of antibody molecules
Abner Louis Notkins
Experimental Medicine Section, Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research NIH, Bethesda, MD 20892 4322, USA
Trends Immunol 25:174-9. 2004
- Quantification of the major allergen from cypress (Cupressus arizonica) pollen, Cup a 1, by monoclonal antibody-based ELISA
M C Arilla
Research and Development Department, Bial Aristegui, Bilbao, Spain
Int Arch Allergy Immunol 134:10-6. 2004
..The aim of this study is to develop a sandwich ELISA for the quantification of Cup a 1, the major allergen of cypress (Cupressus arizonica) pollen extract...
- Determination of the binding parameters for a pre-existing antigen-antibody mixture
S A Bobrovnik
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv
Ukr Biokhim Zh 75:107-14. 2003
..Such a method could be useful for characterization of infection and autoimmune processes when the antigen and antibodies circulate together in the bloodstream...
- Antibody therapy for Alzheimer's disease
Dept of Pharmacology, University of South Florida, Tampa, FC 33612 4799, USA
Expert Rev Vaccines 2:53-9. 2003
..This review summarizes the rationale, progress and setbacks regarding the use of antibody-based therapies to treat Alzheimer's disease and discusses future directions for this approach in Alzheimer's and other disorders...
- Antibody multispecificity mediated by conformational diversity
Leo C James
Centre for Protein Engineering, Medical Research Council Centre, Hills Road, Cambridge CB2 2HQ, UK
Science 299:1362-7. 2003
..Conformational diversity, whereby one sequence adopts multiple structures and multiple functions, can increase the effective size of the antibody repertoire but may also lead to autoimmunity and allergy...
- Monoclonal antibodies as a probe for the unfolding of porcine growth hormone
Dipartimento di Patologia Animale, Igiene e Sanita Pubblica Veterinaria, Sezione di Biochimica e Fisiologia Veterinaria, University of Milan, Via Celoria 10, Italy
J Immunol Methods 272:107-15. 2003
..e. immunoblotting and immunohistochemistry; 3) the performances of the other eight mAbs differed significantly in the competitive and non-competitive ELISA...
- A hypoallergenic vaccine obtained by tail-to-head restructuring of timothy grass pollen profilin, Phl p 12, for the treatment of cross-sensitization to profilin
Christian Doppler Laboratory for Allergy Research, Division of Immunopathology, Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria
J Immunol 179:7624-34. 2007
..The strategy of tail-to-head reassembly of hypoallergenic allergen fragments within one molecule represents a generally applicable strategy for the generation of allergy vaccines...
- Four different clones of mouse anti-acetyllysine monoclonal antibodies having different recognition properties share a common immunoglobulin framework structure
CREST Research Project, Japan Science and Technology Corporation, Kawaguchi Center Building, 4 1 8 Hon cho, Saitama 332 0012, Japan
J Immunol Methods 272:161-75. 2003
- Use of monoclonal antibodies that recognize p60 for identification of Listeria monocytogenes
Kang Y Yu
KOMED Institute for Life Science, Graduate School of Biotechnology, Korea University, Rm 640, 1, 5 Ka, Anam Dong, Sungbuk Ku, Seoul 136 701, Korea
Clin Diagn Lab Immunol 11:446-51. 2004
..Appl. Environ. Microbiol. 60:3120-3127, 1997). To our best knowledge, this is the first example of an immunological identification system that uses p60-recognizing MAbs...
- On molecular recognition of an uranyl chelate by monoclonal antibodies
Jean Marie Teulon
CEA VALRHO, DSV iBEB SBTN, BP 17171, 30207 Bagnols sur Ceze, France
J Mol Recognit 20:508-15. 2007
..In addition, the unbinding process goes through two steps; the first weakens the bonding of the central metal with AspL50 of the antibody and the second breaks other non-bonded interactions of the compound with the antibody...
- Critical contribution of VH-VL interaction to reshaping of an antibody: the case of humanization of anti-lysozyme antibody, HyHEL-10
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai 980 8579, Japan
Protein Sci 17:261-70. 2008
- A direct antigen-binding assay for detection of antibodies against native epitopes using alkaline phosphatase-tagged proteins
Laboratory of Immunogenetics, Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Lavrentyev ave 10, Novosibirsk 630090, Russia
J Immunol Methods 332:73-81. 2008
..The results obtained show that AP-tagged proteins are useful tools for discrimination of antibodies against native epitopes when production of antigen in its native conformation is laborious and expensive...
- A rapid and efficient strategy to generate antigen-specific human monoclonal antibody by in vitro immunization and the phage display method
Shin ei Matsumoto
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6 10 1 Hakozaki, Higashi ku, Fukuoka 812 8581, Japan
J Immunol Methods 332:2-9. 2008
..We thus established a rapid, efficient method to obtain antigen-specific human antibody genes and produce human monoclonal IgG antibody using the phage antibody library generated from in vitro immunized PBMCs...
- Prostate-specific antigen detection by using a reusable amperometric immunosensor based on reversible binding and leasing of HRP-anti-PSA from phenylboronic acid modified electrode
Jiangsu Provincial Key Laboratory of Biomaterials and Biodevices, Southeast University, Nanjing, 210096, People s Republic of China
Clin Chim Acta 395:51-6. 2008
..It is important to develop reusable immunologically-sensitive elements for prostate-specific antigen (PSA) detection...
- Analysis of bispecific monoclonal antibody binding to immobilized antigens using an optical biosensor
D A Dmitriev
Department of Chemical Enzymology, School of Chemistry, Lomonosov Moscow State University, Moscow, 119992 Russia
Biochemistry (Mosc) 67:1356-65. 2002
..In general, the data obtained indicate that Babs bearing an enzyme-binding site may not be efficiently used instead of traditional antibody-enzyme conjugates in the case of binding of bivalent Mabs...
- Antibodies elicited by the secretions from schistosome cercariae and eggs are predominantly against glycan epitopes
T M Kariuki
Institute of Primate Research, National Museums of Kenya, Nairobi
Parasite Immunol 30:554-62. 2008
..Our data further support the hypothesis that antiglycan responses are a smokescreen with negligible protective potential...
- Immunology. Isomeric antibodies
Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Science 299:1327-8. 2003
- TNT detection using multiplexed liquid array displacement immunoassays
George P Anderson
Center for Bio Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375, USA
Anal Chem 78:2279-85. 2006
..Seawater required dilution with two parts buffer to avoid loss of microspheres, while the acetone extracts were diluted 100-fold or more to minimize solvent affects...
- Heat shock protein and gliadin peptide promote development of peptidase antibodies in children with autism and patients with autoimmune disease
Section of Neuroimmunology, Immunosciences Lab, Inc, 8693 Wilshire Blvd, Suite 200, Beverly Hills, CA 90211, USA
Clin Diagn Lab Immunol 11:515-24. 2004
..Dysfunctional membrane peptidases and autoantibody production may result in neuroimmune dysregulation and autoimmunity...
- Airway biofilms: implications for pathogenesis and therapy of respiratory tract infections
First Department of Internal Medicine, Kyorin University School of Medicine, Shinkawa, Mitaka, Tokyo, Japan
Treat Respir Med 4:241-53. 2005
..The harmful reactions are mediated by alginate, which induces antigen antibody reactions around the airways, as well as formation of circulating immune complexes that are deposited on lung ..
- Real-time label-free detection of complement activation products in human serum by white light reflectance spectroscopy
Panagiota S Petrou
Institute of Radioisotopes and Radiodiagnostic Products, NCSR Demokritos, GR 15310 Aghia Paraskevi, Greece
Biosens Bioelectron 24:3359-64. 2009
..Thus, this novel assay method can be used to monitor serum C3b produced as a result of complement activation in a variety of normal and pathologic conditions...
- [Pharmacological and clinical properties of levocabastine hydrochloride (eye drop and nasal spray), a selective H1 antagonist]
Nonclinical Group, Janssen Pharmaceutical K K, 1 5 Higashi gotanda 3 chome, Shinagawa ku, Tokyo 141 8633, Japan
Nihon Yakurigaku Zasshi 119:175-84. 2002
..With these pharmacological effects and topical usage, levocabastine was shown to be useful for allergic conjunctive and rhinitis in both seasonal and perennial clinical use...
- Inhibitory mechanism of aloe single component (alprogen) on mediator release in guinea pig lung mast cells activated with specific antigen-antibody reactions
J Y Ro
Department of Pharmacology, Yonsei University College of Medicine, Seoul, Korea
J Pharmacol Exp Ther 292:114-21. 2000
- Terpenoids and glycolipids from euphorbiaceae
Department of Pharmaceutical Sciences, University of Trieste, Piazzale Europa 1, 34127 Trieste, Italy
Mini Rev Med Chem 3:425-37. 2003
..These molecules may function as endogenous modulators of cell function and possibly as second messengers...
- ABO reverse grouping: effect of varying concentrations of the enzyme bromelain
L E Rookard
Donor Testing Centre, National Blood Service, Brentwood, Essex, UK
Br J Biomed Sci 66:93-7. 2009
..This study identified limitations and problems with the methods used and presents recommendations for future research which may assist in the clarification of the role of proteolytic enzymes in ABO reverse grouping...
- Capacitive monitoring of protein immobilization and antigen-antibody reactions on monomolecular alkylthiol films on gold electrodes
V M Mirsky
University of Regensburg, Institute of Analytical Chemistry, Chemo and Biosensors, Regensburg, Germany
Biosens Bioelectron 12:977-89. 1997
..Specifically, antibodies to human serum albumin were immobilized on the alkylthiol mono-layer. Binding of the antigen led to a decrease of the electrode capacitance. The detection limit of the immunosensor is as low as 15 nM (1 mg/l)...
- Inhibitory effect of levocabastine on experimental allergic conjunctivitis in guinea pigs
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan
J Pharmacobiodyn 14:467-73. 1991
..Furthermore, levocabastine prevented an increase in the vascular permeability elicited by both Hi and antigen instillation...
- Inhibitory effect of ginsenoside on the mediator release in the guinea pig lung mast cells activated by specific antigen-antibody reactions
J Y Ro
Department of Pharmacology, Yonsei University, College of Medicine, Seoul, Korea
Int J Immunopharmacol 20:625-41. 1998
..Furthermore, Rb1 reduces the phosphatidylcholine production by inhibiting the methyl-transferase I and II, and the reduction of phosphatidylcholine production inhibits leukotriene release...
- Localization of telomerase hTERT protein in frozen sections of basal cell carcinomas (BCC) and tumor margin tissues
Eva Maria Fabricius
Charite Universitatsmedizin, Campus Virchow Hospital, Clinic for Oral and Maxillofacial Surgery, Berlin, Germany
Int J Oncol 35:1377-94. 2009
..Finally, we compared the hTERT expression in BCC tissues with the hTERT scores in HNSCC tissues from our previous study. Only one anti-hTERT antibody (our Ab 7) yielded significantly higher scores in BCC than in HNSCC...
- A new concept of accommodation in ABO-incompatible kidney transplantation
Division of Urology, Department of Regenerative and Transplant Medicine, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan
Clin Transplant 19:76-85. 2005
..In other words, bone marrow-derived histo-blood type regulates the histo-blood type of the organs...
- [Severe adverse event following iv administration of 10 ml 6% Dextran60 (0.6 g) in a healthy volunteer]
Institut für Experimentelle Anästhesiologie der Johann Wolfgang Goethe Universität Frankfurt, Germany
Anaesthesist 51:820-4. 2002
..e. 0.6 g) are infused. This is becoming more and more important due to the increasing use of "small volume resuscitation", solutions containing dextran (RescueFlow((R)), Biophausia AB, Uppsala, Schweden) or cryoconservation with dextran...
- Membrane-associated carbonic anhydrase IV in skeletal muscle: subcellular localization
Abteilung Zellbiologie und Elektronenmikroskopie, Medizinische Hochschule Hannover, Germany
Histochem Cell Biol 106:405-11. 1996
..Conceivably, the presence of SR staining in ultrathin sections and its absence in semithin sections reflect a problem of accessibility of the antigenic sites...
- Immunohistochemical study on the chicken adenohypophysis using monoclonal mouse anti-human FSH as a primary antibody
Department of Histology and Embryology, Uludağ University School of Veterinary, Bursa, Turkey
Anat Histol Embryol 33:49-52. 2004
..Secondly, the incubation with the primary antibody (dilution 1/100 for anti-FSH) at 37 degrees C for 90 min...
- Current issues in species identification for forensic science and the validity of using the cytochrome oxidase I (COI) gene
ANZPAA National Institute of Forensic Science, Melbourne, Vic, Australia
Forensic Sci Med Pathol 6:233-41. 2010
..techniques commonly utilized in Australian Forensic Science laboratories are gel immunodifussion antigen antibody reactions and hair comparison analysis...
- Protamine-associated hypotension in patients on hemodialysis: retrospective study and prevalence of antiprotamine antibodies
Y T Tsai
Division of Cardiovascular Surgery, Department of Surgery, Tri Service General Hospital, Taipei, Taiwan, ROC
Clin Nephrol 72:122-8. 2009
..The objective of this study is to investigate whether patients on hemodialysis are more susceptible to the protamine adverse effects...
- Operation of acoustic plate mode immunosensors in complex biological media
Angewandte Physikalische Chemie, Universitaet Heidelberg, Germany
Anal Chem 71:3150-6. 1999
..While the observed frequency shifts are comparable to those measured in pure buffer solutions, the binding process is accompanied by additional acoustic loss, indicating changes in the viscoelastic properties of the interfacial layer...
- RNA aptamer-based optical nanostructured sensor for highly sensitive and label-free detection of antigen-antibody reactions
Ha Minh Hiep
Department of Applied Physics, Graduate School of Engineering, Osaka University, Suita, Osaka 565 0871, Japan
Anal Bioanal Chem 396:2575-81. 2010
..1 ng/mL. These nanostructured sensor-based models will open a way to display numerous immunosensors as well as to develop other functionally similar sensors which could then be expanded into multi-arrays assay systems...
- [Advances in identification of semen stains]
Guang Yao Fan
School of Forensic Medicine, Henan University of Science and Technology, Luoyang, Henan 471003, China
Zhonghua Nan Ke Xue 16:735-40. 2010
..Further more, studies on tis-sue-specific differential DNA methylation will provide a scrumptious way of identifying difficult samples...
- Capacitive monitoring of the antigen-antibody reactions enhanced by nanogold
X S Guo
Biosensor National Special Laboratory, Department of Biomedical Engineering Department of Biosystems Engineering, Zhejiang University, Hang Zhou 310027, P R China E mail Fax 86 571 87951676
Conf Proc IEEE Eng Med Biol Soc 2:1260-3. 2005
..The result showed the capacity of the electrical double layer by nanogold enhancement was largely increased compared to that in traditional capacitive immunosensor. The detection sensitivity is up to 10nF / μg/ml...
- Loop mediated isothermal amplification combined with nucleic acid lateral flow strip for diagnosis of cyprinid herpes virus-3
Fish Medicine and Livestock Management, University of Veterinary Medicine, Vienna, Austria
Mol Cell Probes 24:38-43. 2010
- Immunohistological evaluation of immune cell infiltrate in cutaneous Kaposi's sarcoma
Mahmoud R Hussein
Department of Pathology, Assiut University Hospitals, Faculty of Medicine, Assiut University, Assiut, Egypt
Cell Biol Int 32:157-62. 2008
..However, these issues should be tested by further studies...
- Comparison of blocking agents for an ELISA for LPS
Department of Medical Microbiology and Immunology, University Medical School of Pecs, Hungary
J Immunoassay 21:341-54. 2000
..This modification increases the sensitivity of ELISA. This method is useful for detection of LPS (S, R form) and anti-LPS antibody reaction in serological cross-reaction studies...
- Development of an ultrasound-emitting device for performing rapid immunostaining procedures
Department of Diagnostic Pathology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930 0194, Japan
J Histochem Cytochem 58:421-8. 2010
..Finally, we tried immunostaining with the ultrasonic generator using frozen specimens to simulate intraoperative rapid diagnosis. We report herein that ultrasound enables immunostaining of frozen specimens in approximately 10 min...
- Characterization of the epitope recognized by a monoclonal antibody highly specific for blood group M antigen
Department of Immunochemistry, Polish Academy of Sciences, Wroclaw
Transfusion 30:230-5. 1990
..The reaction of the antibody with blood group N antigen was not detectable in any of the assays used...
- Antigen-antibody binding kinetics for biosensor applications. A dual-fractal analysis
Chemical Engineering Department, University of Mississippi 38677 9740, USA
Appl Biochem Biotechnol 62:275-90. 1997
- Multichannel wireless-electrodeless quartz-crystal microbalance immunosensor
Graduate School of Engineering Science, Osaka University, Machikaneyama 1 3, Toyonaka, Osaka 560 8531, Japan
Anal Chem 82:3957-62. 2010
..The exponential coefficient of the frequency change, rather than the amount of the frequency decrease, is found to be useful for distinguishing between specific and nonspecific binding reactions...
- Nephritogenicity and immunocytochemical localization of the 70-kilodalton glycoprotein subunit (gp70) of Heymann antigen
A K Singh
Department of Nephrology, Rush Medical College, Chicago, Illinois 60612
Clin Immunol Immunopathol 48:61-77. 1988
..The presence of gp70 on the endothelial side of the GBM is consistent with a role for in situ antigen-antibody reactions at sites other than the subepithelial region in the pathogenesis of HN...
- A classification of drug substances according to their mechanism of action
Fachbereich Pharmazie, Philipps Universitat, Marburg, Germany
Pharmazie 59:579-89. 2004
..This target-oriented approach may be particularly useful in teaching advanced medicinal chemistry...
- Use of a 203 alphaalpha fragment of Tc-PDI to detect IgA activity during infection by Teladorsagia circumcincta in sheep
Departamento de Sanidad Animal, Parasitologia, Facultad de Veterinaria, Universidad de Leon, 24071 Leon, Spain
Parasitol Res 100:1213-20. 2007
..Nevertheless, the ELISA technique using the somatic antigen cannot be standardized, and the antigen-antibody reactions are more unspecific; therefore, the recombinant Tc-PDI could be a better option...
- Design and simulation of active biochip system
Institute of Genetic Diagnosis, The Fourth Military Medical University, Xi an 710032, PR China
Biomed Microdevices 7:157-60. 2005
..Computational simulation demonstrated that this system is a rapid, stable, robust and practical system that may enhance the efficiency of antigen-antibody reactions and improve the repeatability and accuracy of biochip analysis...
- Antimitochondrial antibodies and reactivity to N. aromaticivorans proteins in Icelandic patients with primary biliary cirrhosis and their relatives
Department of Internal Medicine, Landspitali University Hospital, Reykjavik, Iceland
Am J Gastroenterol 99:2143-6. 2004
..aromaticivorans might be the trigger of autoimmunity in PBC. No data are available on the specificity and crossreactivity of AMA in a genetically homogenous group of patients, such as the Icelandic population...
- A novel competitive fluorescence immunoassay for the determination of dibutyl phthalate
Ming Cui Zhang
College of Environmental Science and Engineering, Donghua University, Shanghai 201620, People s Republic of China
Anal Bioanal Chem 386:1401-6. 2006
- Characterization of antibody-antigen interactions by fluorescence spectroscopy
Department of Chemistry, The Open University, Milton Keynes, UK
Methods Mol Biol 248:431-41. 2004
- Label-free biomarker detection from whole blood
Department of Biomedical Engineering, School of Engineering and Applied Science, Yale University, New Haven, Connecticut 06511, USA
Nat Nanotechnol 5:138-42. 2010
..This study marks the first use of label-free nanosensors with physiological solutions, positioning this technology for rapid translation to clinical settings...
- Perspectives on the neutralization of interferons by antibody
Sidney E Grossberg
Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI, USA
Neurology 61:S21-3. 2003
- Antibodies use heme as a cofactor to extend their pathogen elimination activity and to acquire new effector functions
Jordan D Dimitrov
Department of Immunology, Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Bonchev Street, 1113 Sofia, Bulgaria
J Biol Chem 282:26696-706. 2007
..The potentiation of the antibacterial activity of IgG after contact with heme may represent a novel and inducible innate-type defense mechanism against invading pathogens...
- Effective antigen-retrieval method for immunohistochemical detection of abnormal isoform of prion proteins in animals
Department of Pathobiological Science, Obihiro University of Agriculture and Veterinary Medicine, 080 8555, Obihiro, Japan
Acta Neuropathol 109:263-71. 2005
..Based on the differences of reaction among the antibodies, we also discuss the mechanisms of the hydrated autoclaving methods to retrieve PrP(Sc) immunoreactivity...