antibody specificity

Summary

Summary: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.

Top Publications

  1. ncbi Antibody neutralization and escape by HIV-1
    Xiping Wei
    Howard Hughes Medical Institute, University of Alabama at Birmingham, 720 South 20th Street, Kaul 816, Birmingham, Alabama 35294 0024, USA
    Nature 422:307-12. 2003
  2. ncbi Antibody domain exchange is an immunological solution to carbohydrate cluster recognition
    Daniel A Calarese
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 300:2065-71. 2003
  3. ncbi Predominant autoantibody production by early human B cell precursors
    Hedda Wardemann
    Laboratory of Molecular Immunology, Rockefeller University, New York, NY 10021, USA
    Science 301:1374-7. 2003
  4. pmc Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning
    Thomas Tiller
    Max Planck Institute for Infection Biology, D 10117 Berlin, Germany
    J Immunol Methods 329:112-24. 2008
  5. pmc A limited number of antibody specificities mediate broad and potent serum neutralization in selected HIV-1 infected individuals
    Laura M Walker
    Department of Immunology and Microbial Science and IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, California, United States of America
    PLoS Pathog 6:e1001028. 2010
  6. ncbi Influenza A vaccine based on the extracellular domain of M2: weak protection mediated via antibody-dependent NK cell activity
    Andrea Jegerlehner
    Cytos Biotechnology, Schlieren, Switzerland
    J Immunol 172:5598-605. 2004
  7. pmc Antibody specificities associated with neutralization breadth in plasma from human immunodeficiency virus type 1 subtype C-infected blood donors
    Elin S Gray
    AIDS Virus Research Unit, National Institute for Communicable Diseases, Johannesburg, Private Bag X4, Sandringham 2131, Johannesburg, South Africa
    J Virol 83:8925-37. 2009
  8. ncbi Broadly neutralizing anti-HIV antibody 4E10 recognizes a helical conformation of a highly conserved fusion-associated motif in gp41
    Rosa M F Cardoso
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Immunity 22:163-73. 2005
  9. pmc Specificity controls for immunocytochemistry: the antigen preadsorption test can lead to inaccurate assessment of antibody specificity
    Silvia Holmseth
    Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
    J Histochem Cytochem 60:174-87. 2012
  10. pmc Beta-lactamase inhibitors derived from single-domain antibody fragments elicited in the camelidae
    K E Conrath
    Department of Ultrastructure, Vrije Universiteit Brussel, Pardenstraat 65, B 1640 St Genesius Rode, Belgium
    Antimicrob Agents Chemother 45:2807-12. 2001

Detail Information

Publications278 found, 100 shown here

  1. ncbi Antibody neutralization and escape by HIV-1
    Xiping Wei
    Howard Hughes Medical Institute, University of Alabama at Birmingham, 720 South 20th Street, Kaul 816, Birmingham, Alabama 35294 0024, USA
    Nature 422:307-12. 2003
    ..The evolving glycan shield thus represents a new mechanism contributing to HIV-1 persistence in the face of an evolving antibody repertoire...
  2. ncbi Antibody domain exchange is an immunological solution to carbohydrate cluster recognition
    Daniel A Calarese
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 300:2065-71. 2003
    ..The unique interdigitation of Fab domains within an antibody uncovers a previously unappreciated mechanism for high-affinity recognition of carbohydrate or other repeating epitopes on cell or microbial surfaces...
  3. ncbi Predominant autoantibody production by early human B cell precursors
    Hedda Wardemann
    Laboratory of Molecular Immunology, Rockefeller University, New York, NY 10021, USA
    Science 301:1374-7. 2003
    ..Most of these autoantibodies were removed from the population at two discrete checkpoints during B cell development. Inefficient checkpoint regulation would lead to substantial increases in circulating autoantibodies...
  4. pmc Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning
    Thomas Tiller
    Max Planck Institute for Infection Biology, D 10117 Berlin, Germany
    J Immunol Methods 329:112-24. 2008
    ....
  5. pmc A limited number of antibody specificities mediate broad and potent serum neutralization in selected HIV-1 infected individuals
    Laura M Walker
    Department of Immunology and Microbial Science and IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, California, United States of America
    PLoS Pathog 6:e1001028. 2010
    ..In approximately half of the donors, key N-linked glycans were critical for expression of the epitopes recognized by the bNAb specificities in the sera...
  6. ncbi Influenza A vaccine based on the extracellular domain of M2: weak protection mediated via antibody-dependent NK cell activity
    Andrea Jegerlehner
    Cytos Biotechnology, Schlieren, Switzerland
    J Immunol 172:5598-605. 2004
    ....
  7. pmc Antibody specificities associated with neutralization breadth in plasma from human immunodeficiency virus type 1 subtype C-infected blood donors
    Elin S Gray
    AIDS Virus Research Unit, National Institute for Communicable Diseases, Johannesburg, Private Bag X4, Sandringham 2131, Johannesburg, South Africa
    J Virol 83:8925-37. 2009
    ....
  8. ncbi Broadly neutralizing anti-HIV antibody 4E10 recognizes a helical conformation of a highly conserved fusion-associated motif in gp41
    Rosa M F Cardoso
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Immunity 22:163-73. 2005
    ..These structural insights should assist in the design of immunogens to elicit 4E10-like neutralizing responses...
  9. pmc Specificity controls for immunocytochemistry: the antigen preadsorption test can lead to inaccurate assessment of antibody specificity
    Silvia Holmseth
    Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
    J Histochem Cytochem 60:174-87. 2012
    ..In conclusion, they argue against uncritical use of the preadsorption test and, in doing so, address a number of other issues related to immunocytochemistry specificity testing...
  10. pmc Beta-lactamase inhibitors derived from single-domain antibody fragments elicited in the camelidae
    K E Conrath
    Department of Ultrastructure, Vrije Universiteit Brussel, Pardenstraat 65, B 1640 St Genesius Rode, Belgium
    Antimicrob Agents Chemother 45:2807-12. 2001
    ..This innovative strategy could generate multiple potent inhibitors for all types of beta-lactamases...
  11. pmc The immunogenicity of humanized and fully human antibodies: residual immunogenicity resides in the CDR regions
    Fiona A Harding
    Facet Biotech, Redwood City, CA, USA
    MAbs 2:256-65. 2010
    ..Modifications to CDR regions can be designed that reduce the immunogenic potential while maintaining the bioactivity of the antibody molecule...
  12. pmc Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen
    Kenneth Smith
    Department of Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Nat Protoc 4:372-84. 2009
    ..Although dependent on having an ongoing immune response, the approach described herein can be used to rapidly generate numerous antigen-specific hmAbs in a short time...
  13. ncbi The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies
    Hiroshi Kimura
    Nuclear Function and Dynamics Unit, HMRO, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Cell Struct Funct 33:61-73. 2008
    ..These highly-reliable and fully-characterized monoclonal antibodies may facilitate future epigenomic studies on healthy and diseased cells...
  14. pmc Rapid cloning of high-affinity human monoclonal antibodies against influenza virus
    Jens Wrammert
    Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Nature 453:667-71. 2008
    ..However, we found that most of the influenza-virus-specific mAbs showed the highest affinity for the current vaccine strain. Thus, OAS does not seem to be a common occurrence in normal, healthy adults receiving influenza vaccination...
  15. pmc Ten commercial antibodies for alpha-1-adrenergic receptor subtypes are nonspecific
    Brian C Jensen
    Cardiology Section and Research Service, San Francisco VA Medical Center 111 C 8, 4150 Clement St, San Francisco, CA 94121, USA
    Naunyn Schmiedebergs Arch Pharmacol 379:409-12. 2009
    ..These results raise caution with prior studies using these reagents. For now, competition radioligand binding is the only reliable approach to quantify the alpha1-AR subtype proteins. Receptor protein localization remains a challenge...
  16. pmc An assessment of histone-modification antibody quality
    Thea A Egelhofer
    Department of Molecular, Cell and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, USA
    Nat Struct Mol Biol 18:91-3. 2011
    ..We advise rigorous testing of histone-modification antibodies before use, and we provide a website for posting new test results (http://compbio.med.harvard.edu/antibodies/)...
  17. ncbi Efficient neutralization of primary isolates of HIV-1 by a recombinant human monoclonal antibody
    D R Burton
    Department of Immunology, Scripps Research Institute, La Jolla, CA 92037
    Science 266:1024-7. 1994
    ..The broad specificity and efficacy of the antibody implies the conservation of a structural feature on gp120, which could be important in vaccine design...
  18. pmc The role of antibody polyspecificity and lipid reactivity in binding of broadly neutralizing anti-HIV-1 envelope human monoclonal antibodies 2F5 and 4E10 to glycoprotein 41 membrane proximal envelope epitopes
    S Munir Alam
    Department of Medicine, Duke Human Vaccine Institute, Duke University School of Medicine, RP1 Circuit Drive, Durham, NC 27710, USA
    J Immunol 178:4424-35. 2007
    ..Taken together, these data demonstrate the similarity of 2F5 and 4E10 mAbs to known anti-cardiolipin Abs and support the model that mAb 2F5 and 4E10 binding to HIV-1 involves both viral lipid membrane and gp41 membrane proximal epitopes...
  19. pmc Immunoglobulin domain crossover as a generic approach for the production of bispecific IgG antibodies
    Wolfgang Schaefer
    Biologics Research, Pharma Research and Early Development, Roche Diagnostics GmbH, D 82372 Penzberg, Germany
    Proc Natl Acad Sci U S A 108:11187-92. 2011
    ..Because of its superior side-product profile, the CrossMab(CH1-CL) was selected for in vivo profiling and showed potent antiangiogenic and antitumoral activity...
  20. ncbi Molecular and clinical dissection of CD24 antibody specificity by a comprehensive comparative analysis
    Glen Kristiansen
    Institute of Clinical Pathology, University Hospital Zurich, Zurich, Switzerland
    Lab Invest 90:1102-16. 2010
    ..Our results shed new light on the tumorbiological role of CD24 and resolve discrepancies in the literature related to the use of different CD24 mAbs...
  21. pmc Heterosubtypic neutralizing monoclonal antibodies cross-protective against H5N1 and H1N1 recovered from human IgM+ memory B cells
    Mark Throsby
    Crucell Holland BV, Leiden, The Netherlands
    PLoS ONE 3:e3942. 2008
    ..Furthermore, there are 16 different HA subtypes and no certainty the next pandemic will be caused by an H5 subtype, thus it is important to develop prophylactic and therapeutic interventions that provide heterosubtypic protection...
  22. ncbi A rapid and efficient single-cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood
    Aishun Jin
    Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
    Nat Med 15:1088-92. 2009
    ..Our method can open the way for the generation of therapeutic antibodies for individual patients...
  23. ncbi Domain antibodies: proteins for therapy
    Lucy J Holt
    Domantis Limited, Granta Park, Abington, Cambridge CB1 6GS, UK
    Trends Biotechnol 21:484-90. 2003
    ..dAbs are bioactive as monomers and, owing to their small size and inherent stability, can be formatted into larger molecules to create drugs with prolonged serum half-lives or other pharmacological activities...
  24. pmc Broadly neutralizing antibodies targeted to the membrane-proximal external region of human immunodeficiency virus type 1 glycoprotein gp41
    M B Zwick
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Virol 75:10892-905. 2001
    ..g., subtypes B, C, and E). The results suggest that a rather extensive region of gp41 close to the transmembrane domain is accessible to neutralizing Abs and could form a useful target for vaccine design...
  25. pmc The cation efflux transporter ZnT8 (Slc30A8) is a major autoantigen in human type 1 diabetes
    Janet M Wenzlau
    Barbara Davis Center for Childhood Diabetes, University of Colorado at Denver and Health Sciences Center, 1775 North Ursula Street, Aurora, CO 80045, USA
    Proc Natl Acad Sci U S A 104:17040-5. 2007
    ..The combination of bioinformatics and molecular engineering used here will potentially generate other diabetes autoimmunity markers and is also broadly applicable to other autoimmune disorders...
  26. pmc Accelerated development of IgG autoantibodies and autoimmune disease in the absence of secreted IgM
    M Boes
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 97:1184-9. 2000
    ..These findings suggest that secreted IgM, including IgM autoantibodies produced naturally or as part of an autoimmune response, may lessen the severity of autoimmune pathology associated with IgG autoantibodies...
  27. ncbi Analysis of the antigen combining site: correlations between length and sequence composition of the hypervariable loops and the nature of the antigen
    Abigail V J Collis
    School of Animal and Microbial Sciences, University of Reading, Whiteknights, P O Box 228, UK
    J Mol Biol 325:337-54. 2003
    ..As well as helping us to understand the process of antigen recognition, autoimmune disease and cross-reactivity, these results are of direct application in the design of antibody phage libraries and modification of affinity...
  28. pmc Autoantibody systems in rheumatoid arthritis: specificity, sensitivity and diagnostic value
    Martinus A M van Boekel
    Department of Biochemistry, University of Nijmegen, PO Box 9101, 6500 HB Nijmegen, The Netherlands
    Arthritis Res 4:87-93. 2002
    ..We conclude that, at present, the antibody response directed to citrullinated antigens has the most valuable diagnostic and prognostic potential for RA...
  29. ncbi Modulation of Notch signaling by antibodies specific for the extracellular negative regulatory region of NOTCH3
    Kang Li
    Tanox, Incorporated, Houston, Texas 77025, USA
    J Biol Chem 283:8046-54. 2008
    ....
  30. ncbi Variants of the antibody herceptin that interact with HER2 and VEGF at the antigen binding site
    Jenny Bostrom
    Department of Protein Engineering, Genentech, 1 DNA Way, South San Francisco, CA 94080, USA
    Science 323:1610-4. 2009
    ..Such "two-in-one" antibodies challenge the monoclonal antibody paradigm of one binding site, one antigen. They could also provide new opportunities for antibody-based therapy...
  31. ncbi A two-in-one antibody against HER3 and EGFR has superior inhibitory activity compared with monospecific antibodies
    Gabriele Schaefer
    Department of Research Oncology, Genentech, Inc, South San Francisco, CA 94080, USA
    Cancer Cell 20:472-86. 2011
    ..Compared with monospecific anti-HER antibodies, MEHD7945A was more broadly efficacious in multiple tumor models, showing that combined inhibition of EGFR and HER3 with a single antibody is beneficial...
  32. ncbi Polyreactivity of antibody molecules
    Abner Louis Notkins
    Experimental Medicine Section, Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research NIH, Bethesda, MD 20892 4322, USA
    Trends Immunol 25:174-9. 2004
  33. pmc Pharmacological characterization of six trkB antibodies reveals a novel class of functional agents for the study of the BDNF receptor
    M Cazorla
    Laboratory of Neurobiology and Molecular Pharmacology, Centre de psychiatrie et Neurosciences, UMR 894 INSERM Université Paris Descartes, 2ter rue d Alesia, Paris, France
    Br J Pharmacol 162:947-60. 2011
    ..In this study, we used an antibody-based approach to search for trkB-selective functional reagents...
  34. pmc Lack of specificity of commercial antibodies leads to misidentification of angiotensin type 1 receptor protein
    Marcela Herrera
    Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, NC, USA
    Hypertension 61:253-8. 2013
    ..We conclude that anti-AT(1)R antibodies available from commercial sources and commonly used in published studies exhibit nonspecific binding in mouse tissue that may lead to erroneous results...
  35. ncbi Behavioral correlates of maternal antibody status among children with autism
    Daniel Braunschweig
    Division of Rheumatology Allergy and Clinical Immunology, University of California, Davis, 451 E Health Sciences Drive, Suite 6510 GBSF, Davis, CA 95616, USA
    J Autism Dev Disord 42:1435-45. 2012
    ..05). This study provides evidence of multiple patterns of reactivity to fetal brain proteins by maternal antibodies associated with ASD and specific childhood behavioral outcomes...
  36. pmc Dynamic antibody specificities and virion concentrations in circulating immune complexes in acute to chronic HIV-1 infection
    Pinghuang Liu
    Duke Human Vaccine Institute, Duke University, Durham, North Carolina, USA
    J Virol 85:11196-207. 2011
    ..virion-immune complexes (ICs), present in approximately 90% of AHI subjects, and compared the levels and antibody specificity to those in chronic infection...
  37. pmc Structurally distinct nicotine immunogens elicit antibodies with non-overlapping specificities
    M Pravetoni
    Minneapolis Medical Research Foundation, 600 Shapiro Bldg, 914 S 8th Street, Minneapolis, MN 55404, USA
    Biochem Pharmacol 83:543-50. 2012
    ..This approach could be applicable to other addiction vaccines or small molecule targets as well...
  38. pmc Discovery of potential diagnostic and vaccine antigens in herpes simplex virus 1 and 2 by proteome-wide antibody profiling
    Mina Kalantari-Dehaghi
    Division of Infectious Diseases, School of Medicine, University of California, Irvine, Irvine, California, USA
    J Virol 86:4328-39. 2012
    ..e., recognized by both HSV-1- and HSV-2-positive donors). Both membrane and nonmembrane virion proteins were antigenic, although type-specific antigens were enriched for membrane proteins, despite being expressed in E. coli...
  39. ncbi Allergen specificity of IgG(4)-expressing B cells in patients with grass pollen allergy undergoing immunotherapy
    Louisa K James
    Randall Division of Cell and Molecular Biophysics, King s College London, London, United Kingdom
    J Allergy Clin Immunol 130:663-670.e3. 2012
    ....
  40. ncbi Efficient inhibition of EGFR signaling and of tumour growth by antagonistic anti-EFGR Nanobodies
    Rob C Roovers
    Department of Molecular Cell Biology, Institute of Biomembranes, Utrecht University, Padualaan 8, CH 3584 Utrecht, The Netherlands
    Cancer Immunol Immunother 56:303-317. 2007
    ..The results show that functional phage antibody selection, coupled to the rational design of Nanobodies, permits the rapid development of novel anti-cancer antibody-based therapeutics...
  41. pmc Human monoclonal antibody combination against SARS coronavirus: synergy and coverage of escape mutants
    Jan Ter Meulen
    Crucell Holland B V, Leiden, Netherlands
    PLoS Med 3:e237. 2006
    ..Combinations of virus-neutralizing, noncompeting mAbs may have these properties...
  42. ncbi Generation of antibodies of distinct subclasses and specificity is linked to H2s in an active mouse model of epidermolysis bullosa acquisita
    Ralf J Ludwig
    Department of Dermatology, University of Lubeck, Lubeck, Germany
    J Invest Dermatol 131:167-76. 2011
    ..Furthermore, our data are the basis for future studies with the goal of identifying non-MHC EBA susceptibility genes...
  43. ncbi Association of G-protein-coupled receptor 154 with asthma and total IgE in a population of the Caribbean coast of Colombia
    C Vergara
    Institute for Immunological Research, University of Cartagena, Cartagena, Colombia
    Clin Exp Allergy 39:1558-68. 2009
    ..It has been subsequently associated with asthma and other inflammatory diseases in several populations with different ethnic origin. Replication of associations adds reliability to these findings...
  44. ncbi De novo donor-specific antibody at the time of kidney transplant biopsy associates with microvascular pathology and late graft failure
    L G Hidalgo
    Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada
    Am J Transplant 9:2532-41. 2009
    ..We propose careful assessment of de novo DSA, particularly against class II, be performed in all late kidney transplant biopsies...
  45. ncbi Is my antibody-staining specific? How to deal with pitfalls of immunohistochemistry
    Jean Marc Fritschy
    Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland
    Eur J Neurosci 28:2365-70. 2008
    ..As a consequence, antibody characterization in immunohistochemistry should include their susceptibility towards fixation and determination of the optimal conditions for their use...
  46. ncbi An integrated approach to extreme thermostabilization and affinity maturation of an antibody
    Audrey D McConnell
    AnaptysBio, Inc, 10421 Pacific Center Court, Suite 200, San Diego, CA 92121, USA
    Protein Eng Des Sel 26:151-64. 2013
    ....
  47. pmc Application of phage display to high throughput antibody generation and characterization
    Darren J Schofield
    Abcam Ltd, Cambridge Science Park, Cambridge CB4 0FW, UK
    Genome Biol 8:R254. 2007
    ..Thus, we have demonstrated the potential of and illuminated the issues associated with genome-wide monoclonal antibody generation...
  48. ncbi Selection of full-length IgGs by tandem display on filamentous phage particles and Escherichia coli fluorescence-activated cell sorting screening
    Yariv Mazor
    Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    FEBS J 277:2291-303. 2010
    ....
  49. ncbi Conserved structural elements in the V3 crown of HIV-1 gp120
    Xunqing Jiang
    Department of Biochemistry, New York University School of Medicine, New York, New York, USA
    Nat Struct Mol Biol 17:955-61. 2010
    ..As these regions are targeted by cross-clade neutralizing human antibodies, they provide a blueprint for the design of vaccine immunogens that could elicit broadly cross-reactive protective antibodies...
  50. ncbi Isolation and characterisation of Ebolavirus-specific recombinant antibody fragments from murine and shark immune libraries
    Sarah A Goodchild
    Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK
    Mol Immunol 48:2027-37. 2011
    ....
  51. ncbi Immunological characteristics associated with the protective efficacy of antibodies to ricin
    Massimo Maddaloni
    Department of Microbiology, Montana State University, Bozeman, MT 59717, USA
    J Immunol 172:6221-8. 2004
    ..Anti-A chain Abs with moderate avidity had no in vivo effect, nor did any anti-B chain Abs...
  52. pmc IgG subclass profiles in infected HIV type 1 controllers and chronic progressors and in uninfected recipients of Env vaccines
    Kaustuv Banerjee
    Department of Microbiology and Immunology, Weill Cornell Medical College, New York, New York 10065, USA
    AIDS Res Hum Retroviruses 26:445-58. 2010
    ..Although we found isotypic differences in IgG responses to HIV-1 antigens among vaccinees and the HC and CP individuals, there were no indications of differential T(H)1:T(H)2 polarization between the different groups...
  53. pmc Evolving concepts of specificity in immune reactions
    Herman N Eisen
    Koch Institute for Integrative Cancer Research, and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 107:22373-80. 2010
    ....
  54. ncbi The carboxy-terminus of merozoite surface protein 1: structure, specific antibodies and immunity to malaria
    A A Holder
    Division of Parasitology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London
    Parasitology 136:1445-56. 2009
    ..Understanding the structure of MSP1 will help us design and engineer better antigens that are more effective than the first generation of vaccine candidates. This review is focused on the carboxy-terminus of MSP1...
  55. ncbi A West Nile virus recombinant protein vaccine that coactivates innate and adaptive immunity
    William F McDonald
    VaxInnate Corporation, New Haven, CT 06511, USA
    J Infect Dis 195:1607-17. 2007
    ..They also support the further development of an effective WNV vaccine and novel monovalent and multivalent vaccines for related flaviviruses...
  56. pmc Role of immune mechanisms in induction of HIV-1 broadly neutralizing antibodies
    Laurent Verkoczy
    Duke Human Vaccine Institute, Duke University Medical Center, Durham, NC, USA
    Curr Opin Immunol 23:383-90. 2011
    ....
  57. ncbi Clinical and laboratory aspects of Ro/SSA-52 autoantibodies
    Caterina Defendenti
    Laboratory Unit, Fatebenefratelli Hospital, Milan, Italy
    Autoimmun Rev 10:150-4. 2011
    ..However, the majority of the specific diagnosis is mostly based on the simultaneous presence of other autoantibodies that seems diagnostically more relevant...
  58. pmc A guide to the perplexed on the specificity of antibodies
    Clifford B Saper
    Department of Neurology, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA
    J Histochem Cytochem 57:1-5. 2009
    ..In this review, I consider the principles of antibody action and how they define a set of rules for what information should be obtained by the investigator before using an antibody in a serious scientific investigation...
  59. ncbi Generation of monoclonal antibody that distinguishes PrPSc from PrPC and neutralizes prion infectivity
    Motohiro Horiuchi
    Laboratory of Prion Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita ku, Sapporo, Hokkadio 060 0818, Japan
    Virology 394:200-7. 2009
    ..Taken together, these results suggest that mAb 6H10 recognizes a conformational epitope on PrP(Sc) that is related to prion infectivity...
  60. pmc Immunodetection of DNA repair endonuclease ERCC1-XPF in human tissue
    Nikhil R Bhagwat
    Department of Human Genetics, University of Pittsburgh School of Public Health, PA, USA
    Cancer Res 69:6831-8. 2009
    ..The most commonly used antibody, 8F1, is not suitable for immunodetection in tissue. The results with validated antibodies reveal marked differences in ERCC1-XPF protein levels between samples and cell types...
  61. ncbi A new perspective: molecular motifs on oxidized LDL, apoptotic cells, and bacteria are targets for chronic lymphocytic leukemia antibodies
    Anna Lanemo Myhrinder
    Department of Clinical and Experimental Medicine, Linkoping University, Sweden
    Blood 111:3838-48. 2008
    ....
  62. pmc Display of VP1 on the surface of baculovirus and its immunogenicity against heterologous human enterovirus 71 strains in mice
    Tao Meng
    Animal Health Biotechnology, Temasek Life Sciences Laboratory, Republic of Singapore
    PLoS ONE 6:e21757. 2011
    ..VP1 is one of the major immunogenic capsid protein of EV71 and plays a crucial role in viral infection. Antibodies against VP1 are important for virus neutralization...
  63. ncbi Reactivity pattern of 92 monoclonal antibodies with 15 human papillomavirus types
    Raeda Z Rizk
    Department of Genome Modifications and Cancer, Infection and Cancer Program, German Cancer Research Center DKFZ, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany
    J Gen Virol 89:117-29. 2008
    ..Distantly related mucosal and skin alpha papillomaviruses share some conformational epitopes and the phylogenetic L1-based species definition may not define a serological unit since no species-specific epitope was found...
  64. ncbi Amyotrophic lateral sclerosis is a non-amyloid disease in which extensive misfolding of SOD1 is unique to the familial form
    Aaron Kerman
    Department of Medical Biophysics, Ontario Cancer Institute, University of Toronto, and Department of Laboratory Medicine and Pathobiology, Toronto General Hospital, TMDT 4 305, 101 College Street, Toronto, ON, M5G 1L7, Canada
    Acta Neuropathol 119:335-44. 2010
    ..This knowledge will provide new insights into subcellular events that cause misfolding, aggregation and toxicity...
  65. ncbi Eosinophilic rhinitis accompanies the development of lower airway inflammation and hyper-reactivity in sensitized mice exposed to aerosolized allergen
    P W Hellings
    Laboratory of Experimental Immunology, Department of Internal Medicine, University Hospitals, Faculty of Medicine, University of Leuven, Herestraat 49, 3000 Leuven, Belgium
    Clin Exp Allergy 31:782-90. 2001
    ..These findings support the viewpoint that upper and lower airway disease represent a continuum of inflammation involving one common airway and provide evidence for the concept of global airway inflammation after inhalation of allergen...
  66. pmc Antigen capture enzyme-linked immunosorbent assay for specific detection of Reston Ebola virus nucleoprotein
    Tetsuro Ikegami
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208 0011, Japan
    Clin Diagn Lab Immunol 10:552-7. 2003
    ..The antigen capture ELISAs using Res2-6C8 and Res2-1D8 are useful for the rapid detection of the NP in EBO-R-infected cynomolgus macaques...
  67. pmc Parasite antigens on the infected red cell surface are targets for naturally acquired immunity to malaria
    P C Bull
    Kenya Medical Research Institute CRC, Kilifi Unit
    Nat Med 4:358-60. 1998
    ..The apparent selective pressure exerted by established anti-PfEMP1 antibodies on infecting parasites supports the idea that such responses provide variant-specific protection against disease...
  68. pmc Post-exposure targeting of specific epitopes on ricin toxin abrogates toxin-induced hypoglycemia, hepatic injury, and lethality in a mouse model
    James K Roche
    Division of Nephrology, Department of Medicine, University of Virginia Health Sciences Center, Charlottesville, VA, USA
    Lab Invest 88:1178-91. 2008
    ..These data identify critical events for liver injury and healing in ricin intoxication, as well as a new means and specific targets for post-exposure therapeutic intervention...
  69. pmc Profiles of human serum antibody responses elicited by three leading HIV vaccines focusing on the induction of Env-specific antibodies
    Michael Vaine
    Laboratory of Nucleic Acid Vaccines, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America
    PLoS ONE 5:e13916. 2010
    ..antibody, neutralizing antibody, antibody-dependent cell-mediated cytotoxicity (ADCC), and profiles of antibody specificity were analyzed for human immune sera collected from vaccinees enrolled in the NIH HIV Vaccine Trial Network (..
  70. pmc Neutralising antibodies against ricin toxin
    Julie Prigent
    CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, CEA Saclay, Gif sur Yvette, France
    PLoS ONE 6:e20166. 2011
    ..5 hours after ricin challenge. The strong in vivo neutralising capacity of this three mAbs combination makes it potentially useful for immunotherapeutic purposes in the case of ricin poisoning or possibly for prevention...
  71. pmc Properties and function of polyreactive antibodies and polyreactive antigen-binding B cells
    Zhao Hua Zhou
    Experimental Medicine Section, Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Building 30, Room 106, 30 Convent Dr, MSC 4322, Bethesda, MD 20892 4322, USA
    J Autoimmun 29:219-28. 2007
    ..In this brief review we will discuss these insights and their impact on basic and clinical immunology...
  72. ncbi New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins
    J Ko
    Biology Division, California Institute of Technology, Pasadena, CA 91125, USA
    Brain Res Bull 56:319-29. 2001
    ..As with MW1-6, the epitopes for MW7 and 8 are differentially available in the various subcellular compartments where Htt is found...
  73. ncbi Expression of laminin isoforms in mouse myogenic cells in vitro and in vivo
    F Schuler
    Institute for Experimental Medicine, University of Edangen Nuremberg, Germany
    J Cell Sci 108:3795-805. 1995
    ..Taken together, the data suggest that laminins 1 and 2 play distinct roles in myogenesis...
  74. pmc Fine and domain-level epitope mapping of botulinum neurotoxin type A neutralizing antibodies by yeast surface display
    R Levy
    Department of Anesthesia and Pharmaceutical Chemistry, University of California, San Francisco, San Francisco General Hospital, CA 94110, USA
    J Mol Biol 365:196-210. 2007
    ..The results demonstrate how yeast display can be used for domain-level and fine mapping of conformational BoNT antibody epitopes and the mapping results identify three neutralizing BoNT/A epitopes...
  75. ncbi The autoantibody repertoire: searching for order
    Paul H Plotz
    Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892 1820, USA
    Nat Rev Immunol 3:73-8. 2003
    ....
  76. pmc Llama single domain antibodies specific for the 7 botulinum neurotoxin serotypes as heptaplex immunoreagents
    Jerry O Conway
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas, United States of America
    PLoS ONE 5:e8818. 2010
    ..Here we employ facile recombinant antibody technology to subtractively select ligands to each of the 7 BoNT serotypes, resulting in populations with very high specificity for their intended serotype...
  77. pmc Expression of the hypoxia-inducible and tumor-associated carbonic anhydrases in ductal carcinoma in situ of the breast
    C C Wykoff
    Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, United Kingdom
    Am J Pathol 158:1011-9. 2001
    ..The existence of differential regulation and associations with an aggressive phenotype may be important in the development of selective inhibitors of CAs, because the latter have recently been shown to prevent tumor invasion...
  78. pmc Antigenicity and immunogenicity of HIV-1 consensus subtype B envelope glycoproteins
    Denise L Kothe
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Virology 360:218-34. 2007
    ....
  79. ncbi Analyzing the "degree of humanness" of antibody sequences
    K R Abhinandan
    Biomolecular Structure and Modelling Unit, Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK
    J Mol Biol 369:852-62. 2007
    ..We have provided a Web server allowing humanness to be assigned for a sequence...
  80. ncbi Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodies
    Barton F Haynes
    Duke University School of Medicine, Durham, NC 27710, USA
    Science 308:1906-8. 2005
    ..These results may have important implications for generating effective neutralizing antibody responses by using HIV-1 vaccines...
  81. pmc Roles of adjuvant and route of vaccination in antibody response and protection engendered by a synthetic matrix protein 2-based influenza A virus vaccine in the mouse
    Krystyna Mozdzanowska
    Immunology Program, The Wistar Institute, Philadelphia, USA
    Virol J 4:118. 2007
    ....
  82. ncbi KChIPs and Kv4 alpha subunits as integral components of A-type potassium channels in mammalian brain
    Kenneth J Rhodes
    Neuroscience, Wyeth Discovery Research, Princeton, New Jersey 08543, USA
    J Neurosci 24:7903-15. 2004
    ....
  83. doi Human leukocyte antigen antibodies and chronic rejection: from association to causation
    Paul I Terasaki
    Transplantation 86:377-83. 2008
    ..The clinical implication is significant because we hope this review will stimulate centers to begin the one remaining task of showing that antibody removal will indeed prevent chronic failure...
  84. ncbi Generation and characterization of anti-MUC4 monoclonal antibodies reactive with normal and cancer cells in humans
    Nicolas Moniaux
    Department of Biochemistry and Molecular Biology and Eppley Institute for Research in Cancer and Allied Disease, University of Nebraska Medical Center, Omaha, Nebraska 68198 4525, USA
    J Histochem Cytochem 52:253-61. 2004
    ..Anti-MUC4 MAb may represent a powerful tool for the study of MUC4 function under normal and pathological conditions and for diagnosis of solid tumors including those in the breast, pancreas, lungs, and ovaries...
  85. pmc Additive effects characterize the interaction of antibodies involved in neutralization of the primary dualtropic human immunodeficiency virus type 1 isolate 89.6
    F Verrier
    Department of Pathology, New York University School of Medicine, New York, New York 10016, USA
    J Virol 75:9177-86. 2001
    ..These studies suggest that Abs rarely act in synergy to neutralize primary isolate HIV-1(89.6); many anti-HIV-1 Abs act additively to mediate this biological function...
  86. ncbi BoLA-DR peptide binding pockets are fundamental for foot-and-mouth disease virus vaccine design in cattle
    R Baxter
    The Roslin Institute and Royal Dick School of Veterinary Studies, The University of Edinburgh, Roslin Biocentre, Midlothian, EH25 9PS, United Kingdom
    Vaccine 28:28-37. 2009
    ....
  87. ncbi Generation and application of type-specific anti-heparan sulfate antibodies using phage display technology. Further evidence for heparan sulfate heterogeneity in the kidney
    T H van Kuppevelt
    Department of Biochemistry, 160, Faculty of Medical Sciences, University of Nijmegen, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    J Biol Chem 273:12960-6. 1998
    ..This is the first time this technique has been successfully applied to obtain directly antibodies to (poly)saccharides...
  88. ncbi Allergy, parasites, and the hygiene hypothesis
    Maria Yazdanbakhsh
    Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands
    Science 296:490-4. 2002
    ..The induction of a robust anti-inflammatory regulatory network by persistent immune challenge offers a unifying explanation for the observed inverse association of many infections with allergic disorders...
  89. ncbi Characterization of a mouse monoclonal antibody specific for O-linked N-acetylglucosamine
    F I Comer
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205-2185, USA
    Anal Biochem 293:169-77. 2001
    ..These studies demonstrate that CTD110.6 is highly specific toward O-GlcNAc, with no cross-reactivity toward similar carbohydrate antigens or toward peptide determinants...
  90. pmc Rabies virus glycoprotein as a carrier for anthrax protective antigen
    Mary Ellen Smith
    Department of Microbiology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Virology 353:344-56. 2006
    ..These data demonstrate that a viral envelope protein can carry a bacterial protein and that a viral carrier can display whole polypeptides compared to the limited epitope presentation of previous viral systems...
  91. ncbi Differential epitope positioning within the germline antibody paratope enhances promiscuity in the primary immune response
    Dhruv K Sethi
    National Institute of Immunology, New Delhi 110067, India
    Immunity 24:429-38. 2006
    ..The observed multiple binding modes of diverse epitopes within a common paratope conformation of a germline antibody reveal a simple, yet elegant, mechanism for expanding the primary antibody repertoire...
  92. pmc Characterization of beta pat-3 heterodimers, a family of essential integrin receptors in C. elegans
    S N Gettner
    Department of Physiology, University of California, San Francisco 94143, USA
    J Cell Biol 129:1127-41. 1995
    ....
  93. pmc Llama-derived single domain antibodies to build multivalent, superpotent and broadened neutralizing anti-viral molecules
    Anna Hultberg
    Department of Biology, Cellular Architecture and Dynamics, University of Utrecht, Utrecht, The Netherlands
    PLoS ONE 6:e17665. 2011
    ..The results show that multimerization of VHH fragments targeting multiple epitopes on a viral trimeric spike protein is a powerful tool for anti-viral therapy to achieve "best-in-class" and broader neutralization capacity...
  94. pmc Effects of feeding Bt MON810 maize to pigs for 110 days on peripheral immune response and digestive fate of the cry1Ab gene and truncated Bt toxin
    Maria C Walsh
    Teagasc, Pig Development Department, Animal and Grassland Research and Innovation Centre, Moorepark, Fermoy, County Cork, Ireland
    PLoS ONE 7:e36141. 2012
    ....
  95. pmc High affinity human antibody fragments to dengue virus non-structural protein 3
    Nicole J Moreland
    Program in Emerging Infectious Diseases, Duke NUS Graduate Medical School, Singapore, Singapore
    PLoS Negl Trop Dis 4:e881. 2010
    ..They are distributed between the N-terminal protease domain in the first one-third and the C-terminal ATPase/helicase and nucleoside 5' triphosphatase domain which forms the remainder of the 618-aa long protein...
  96. pmc Tissue profiling of the mammalian central nervous system using human antibody-based proteomics
    Jan Mulder
    Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
    Mol Cell Proteomics 8:1612-22. 2009
    ..Furthermore optimized tissue processing and colchicine treatment allow a high quality, more extended annotation and detailed analysis of subcellular distributions and protein dynamics...
  97. ncbi Immediate protection of swine from foot-and-mouth disease: a combination of adenoviruses expressing interferon alpha and a foot-and-mouth disease virus subunit vaccine
    Mauro P Moraes
    Plum Island Animal Disease Center, US Department of Agriculture USDA, Agricultural Research Service ARS, P O Box 848, North Atlantic Area NAA, Greenport, NY 11944 0484, USA
    Vaccine 22:268-79. 2003
    ..Swine inoculated with a combination of Ad5-pIFNalpha and Ad5-A24 and challenged 5dpi were all completely protected from disease and developed a significant FMDV-specific neutralizing antibody response...
  98. ncbi Erythropoietin receptor expression in non-small cell lung carcinoma: a question of antibody specificity
    W Mark Brown
    Department of Pathology, Institute of Pathology, Royal Group of Hospitals Trust, Grosvenor Road, Belfast BT12 6BA, United Kingdom
    Stem Cells 25:718-22. 2007
    ....
  99. pmc Preferential use of the VH5-51 gene segment by the human immune response to code for antibodies against the V3 domain of HIV-1
    Miroslaw K Gorny
    Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
    Mol Immunol 46:917-26. 2009
    ..The preferential usage of selected Ig gene segments and the characteristic pattern of Ig gene usage by anti-V3 mAbs can be related to the conserved structure of the V3 region...
  100. pmc Generation of monospecific antibodies based on affinity capture of polyclonal antibodies
    Barbara Hjelm
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, 10691 Stockholm, Sweden
    Protein Sci 20:1824-35. 2011
    ....
  101. ncbi Clinical associations of autoantibodies to a p155/140 kDa doublet protein in juvenile dermatomyositis
    H Gunawardena
    Royal National Hospital for Rheumatic Diseases, Upper Borough Walls, Bath BA1 1RL, UK
    Rheumatology (Oxford) 47:324-8. 2008
    ..Recently, there have been descriptions of novel autoantibodies in DM. This study was conducted to establish the clinical significance of anti-p155/140 autoantibodies in juvenile DM (JDM)...

Research Grants62

  1. Autoantibodies and B Cell Chemotaxis in Sjogren's Syndrome
    JILL MARIE KRAMER; Fiscal Year: 2013
    ..cells subsets responsible for autoantibody production in SS, as well as immunoglobulin gene usage and fine antibody specificity. In specific aims 2 and 3, we will seek to identify innovative therapeutic targets in SS...
  2. Antigen-microarray validated monoclonal antibody library for analysis of brain-ex
    Seth Blackshaw; Fiscal Year: 2010
    ..We have developed an approach to rapidly validate antibody specificity. We propose to use this tool to generate a large set of monoclonal antibodies of verified specificity that ..
  3. Structure and conformational diversity of amyloid oligomers
    Charles G Glabe; Fiscal Year: 2013
    ..the conformational diversity of amyloid aggregates? What is the structural basis of conformation dependent antibody specificity and amyloid oligomer structural? What are the relationships between different conformational states of ..
  4. Immunoprophylaxis to Kell RBC Alloimmunization During Pregnancy
    Jeanne E Hendrickson; Fiscal Year: 2013
    ..limitations of human studies, most of which have been limited to observation/correlation studies between antibody specificity, titer, and fetal outcome...
  5. Synthetic Nanofiber Vaccines for Cocaine Addiction
    Jia Zhou; Fiscal Year: 2013
    ..proper haptens is critical for proper immune stimulation both in terms of amount of antibody elicited and antibody specificity. The goals of this proposal are to design and develop cocaine vaccines based on designed cocaine analogs in ..
  6. Peripheral B Cell Receptor Editing
    Duane R Wesemann; Fiscal Year: 2013
    ..results from this proposal will advance our understanding of the mechanisms of B cell development, antibody specificity modulation and regulation of DNA accessibility to V(D)J rearrangement machinery...
  7. Animal models of autoimmune neuropathy
    BETTY C SOLIVEN; Fiscal Year: 2013
    ..contribute to the development or progression of SAP, and if so, delineate the fine relationship between antibody specificity and pathogenicity...
  8. Antibody Reprtoire Characterization in Celiac Disease
    Patrick S Daugherty; Fiscal Year: 2013
    ..Using high-resolution antibody specificity data, novel self- and non-self antigens will be identified as the targets of serum antibodies that may be ..
  9. PURIFIED TESTIS ANTIGENS AND TESTICULAR AUTOIMMUNITY
    Kenneth Tung; Fiscal Year: 1991
    ..early testicular changes, testicular IgG will be localized by immunoperoxidase technique, and its antibody specificity identified after being eluted with acid pH...
  10. DETERMINATION OF ANTIBODY SPECIFICITY USING AFM
    CHARLES SOKOLIK; Fiscal Year: 2001
    ..The utilization of the AFM to probe libraries made by the ink-jet printer technology will provide for a faster, more reliable and reproducible methodology for uncovering the binding characteristics of an antibody. ..
  11. AFLATOXIN DNA ADDUCTS DETECTED BY MONOCLONAL ANTIBODIES
    John Groopman; Fiscal Year: 1992
    ..made from aflatoxin guanosine adduct produced by synthetic aflatoxin epoxide will be tried to induce an antibody specificity and affinity for the DNA adducts not attained by previous haptenization procedures; (2) Develop analytical ..
  12. BACTERICIDAL ACTIVITY OF LACTOFERRIN ON ORAL FLORA
    Roland Arnold; Fiscal Year: 1993
    ..IgA-LF complex will be isolated and characterized as to the physicochemical nature of the association, its antibody specificity, and its bactericidal activity as it relates to antibody specificity...
  13. RABIES VIRUS AND RABIES VIRUS ANTIGENS
    Hilary Koprowski; Fiscal Year: 2006
    ..elucidate the contributions of antibody specificity and structure and known mechanisms of BBB permeability enhancement to immune protection, using molecular ..
  14. PHASE I STUDY OF ANTICD20/STREPTAVIDIN
    Hazel Breitz; Fiscal Year: 2001
    ..PRIT is a multistep delivery system that takes advantage of antibody specificity and small molecule radioligand pharmacokinetics to increase the therapeutic ratio by reducing the exposure ..
  15. EVOLUTIONARY MODELS OF THE IMMUNE RESPONSE
    Melvin Cohn; Fiscal Year: 1990
    ..various segments of these L and H chains, and even nucleotides that encode amino acids which determine antibody specificity)...
  16. CONTROL OF IMMUNOGLOBULIN SYNTHESIS
    Ursula Storb; Fiscal Year: 1990
    ..A second antibody specificity, anti murine class II MHC molecules will be used to test a model for the determination of T cell MHC ..
  17. Hypocretin System Immune Diathesis in Human Narcolepsy
    John Black; Fiscal Year: 2004
    ..Positive sera and CSF will also be preadsorbed and the studies will be repeated to determine if antibody specificity for these proteins exists...
  18. Integrating Experiment and Theory to Characterize Diagnostic Antibody Specificity
    Robert J Woods; Fiscal Year: 2013
    ....
  19. CHARACTERIZATION OF SEXUAL PHEROMONES IN APLYSIA
    SHERRY PAINTER; Fiscal Year: 1993
    ..provide an immunocytochemical approach to demonstrating release from the atrial gland onto the egg cordon; antibody specificity will be characterized by immunodot assay, immunocytochemistry and preabsorption studies...
  20. Multiplex Microarray for the Detection of Pre-Type 1 Diabetes Autoantibodies
    ZUHEIR AWDEH; Fiscal Year: 2009
    ..Recently, a single ELISA assay that is used to detect two antibodies simultaneously but cannot assign antibody specificity became available...
  21. PATHOGENESIS OF THE GUILLAIN-BARRE SYNDROMES
    John Griffin; Fiscal Year: 1999
    ..response against shared epitopes with very specific localizations in the peripheral nerves, so that antibody specificity dictates the type of nerve fibers involved and th type of pathologic lesion...
  22. Antiphospholipid Antibody Heterogeneity
    Neal Rote; Fiscal Year: 2007
    ..The cloned antibodies will be characterized. Aim 3 will investigate the relationship between antibody specificity and biological activity against trophoblast...
  23. DEVELOPMENT OF THE ANTIBODY REPERTOIRE
    Harry Schroeder; Fiscal Year: 2005
    ..HCDR3 lies at the center of the antigen-binding site and typically determines antibody specificity. At the genetic level, HCDR3 represents the focal point of diversification of the pre-immune B-cell ..
  24. STRUCTURAL STUDIES OF UNIQUE MYELOMA IMMUNOGLOBULINS
    FREDERICK GARVER; Fiscal Year: 1980
    ..in heavy chain disease proteins and (3) the localization of the antibody-combining site and the basis of antibody specificity. We are presently defining the amino acid sequence of three monoclonal immunoglobulins: IgG GAR, IgG Sm and ..
  25. VIIITH INTERNATIONAL CONFERENCE ON MYASTHENIA GRAVIS
    AUDREY PENN; Fiscal Year: 1992
    ..of the thymus gland, the molecular biology of T cell receptors, B cell rearrangements which result in antibody specificity and the biochemistry of glycoprotein receptors...
  26. Immunization-induced AMI and CMI against malaria
    James Burns; Fiscal Year: 2005
    ..We will examine antibody specificity, concentration, isotype and avidity to define measurable parameters that predict protective efficacy...
  27. BLASTULA ADHESION COMPLEXES
    MERRIL HILLE; Fiscal Year: 1990
    ..The complexes will be identified by SDS-PAGE, radiolabeling, antibody specificity, and peptide mapping...
  28. Anthrax Vaccine Formulations Combining PA/Spore Epitopes
    AAIYAPPA PALECANDA; Fiscal Year: 2005
    ..Antibodies will be grouped according to antibody specificity and isotype...
  29. Quantitative Serum Antibody Specificity Screening in Celiac Disease
    Patrick Daugherty; Fiscal Year: 2009
    ..This work will establish a new gold standard methodology for analysis of serum antibody specificities, as well as provide reagents to diagnose a common autoimmune disorder (Celiac Disease), and better understand its cause. ..
  30. BIOCHEMICAL CHARACTERIZATION OF HUMAN IMMUNE NEUTROPENIA
    Mark Currie; Fiscal Year: 1990
    ..in vitro assays listed above along with assays of cytotoxicity and granulocyte function; d) to correlate antibody specificity and consequences of antibody binding to clinical disease...
  31. MOLECULAR CHARACTERIZATION OF C NEOFORMANS ANTIBODIES
    Arturo Casadevall; Fiscal Year: 2006
    ..neoformans appears to be extremely complex. Antibody specificity, isotype and amount have each been shown to be important variables in protective efficacy...
  32. Antibodies Against Amyloid Beta-Protein Intermediates
    ROBERT LAWTON; Fiscal Year: 2003
    ..4) Confirmation of antibody specificity to ABeta PFs by Western blotting and immuno-electron microscopy...
  33. HLA MISMATCH ACCEPTABILITY IN KIDNEY TRANSPLANTATION
    Rene Duquesnoy; Fiscal Year: 1999
    ..Most highly sensitized patients maintain, however, a rather restricted repertoire of antibody specificity towards generally high-frequency antigenic determinants...
  34. ANTIBODY DEPENDENT CELL CYTOTOXICITY REACTIONS
    MICHAEL FANGER; Fiscal Year: 1980
    ..The importance of antibody specificity and affinity in these reactions would be determined, as well as differences in the susceptibility to ..
  35. B CELL & T CELL RECOGNITION SITES OF P FALCIPARUM GP195
    Sandra Chang; Fiscal Year: 1993
    ..by immunization of mice with parasite-purified gp195 and gp195-related polypeptides and evaluation of serum antibody specificity by an ELISA assay...
  36. LINKER-MODULATED DISTRIBUTION OF RADIOIMMUNOCONJUGATES
    Syed Quadri; Fiscal Year: 1993
    ..targeting of radiosensitive normal tissue; (iv) improved clearance from the blood; and (v) undiminished antibody specificity. We now propose to test whether a linker-spacer, which has been shown by our preliminary studies to have ..
  37. IMMUNOPATHOGENESIS OF VISNA
    Neal Nathanson; Fiscal Year: 1980
    ..on (1) description of antigenic drift during longterm infection by characterizing serial isolates and N antibody specificity using specimens already collected...
  38. B Cell Transforation in Fas and FasL deficient Mice
    Wendy Davidson; Fiscal Year: 2004
    ..variable region gene hypermutation and intraclonal variation, restricted use of VH families arid also for antibody specificity. Finally, we will use BALB-gld/gld IL-10 knockout mice to investigate the role of IL- 10 in chronic B cell ..
  39. DETECTION OF FOOD BORNE PATHOGENS
    NEAL SLESZYNSKI; Fiscal Year: 2001
    ..The technology combines antibody specificity, fluorescent label sensitivity, and the ability to physically separate different species of microbes for ..
  40. HUMAN ANTIBODIES TO INTACT VIRIONS FOR VACCINE RESEARCH
    Lisa Cavacini; Fiscal Year: 2000
    ..concerns, identify antibodies that react with native primary isolate virions, and facilitate analysis of antibody specificity and viral neutralization, we have designed an assay using whole virions from primary isolates of HIV-1...
  41. PHYSICAL AND IMMUNOLOGICAL STUDIES OF PROTEIN FOLDING
    Karen Strehlow; Fiscal Year: 1990
    ..We will also have available a library of S. nuclease mutants produced by site-directed mutagenesis and will be able to generate mutants to further probe antibody specificity and the protein folding mechanism.
  42. Assessment of mFLSC(239)complex in cynomologus macaques
    Timothy Fouts; Fiscal Year: 2006
    ..Third, immunization with FLSC must demonstrate efficacy. While the antibody specificity and safety can be investigated in animal models, preclinical demonstration of efficacy with HIV based FLSC ..
  43. Unconventional Myosin Distribution Inner Ear Hair Cells
    ALLISON COFFIN; Fiscal Year: 2004
    ..b>Antibody specificity will be verified with western blotting...
  44. INHIBITING AGGREGATION WITH PHAGE DISPLAY ANTIBODIES
    Michael Sierks; Fiscal Year: 2003
    ..b>Antibody specificity will be increased by subjecting the isolated parent antibody to random mutagenesis of targeted antibody ..
  45. Functions of ERK and p38 MAP Kinases in Astrogliosis
    James Mandell; Fiscal Year: 2008
    ..This approach could promote recovery of brain and spinal cord function after injury or stroke. [unreadable] [unreadable]..
  46. Familial IgA Nephropathy: Genetic and Metabolic Studies
    Jiri Mestecky; Fiscal Year: 2006
    ..abstract_text> ..
  47. Hypoglycemic signaling targets in astrocytes
    James Mandell; Fiscal Year: 2003
    ..The longterm goal is to develop pharmacologic or molecular methods to augment or modulate neuroprotective aspects of the astrocytic response to hypoglycemia. ..
  48. MAP KINASE SIGNALING IN ASTROGLIAL REACTIONS
    James Mandell; Fiscal Year: 2003
    ..3) To generate transgenic mice expressing astrocyte-targeted activators of the ERK/MAPK pathway as critical tests of the working hypothesis. ..
  49. PERIODONTAL DISEASE:ROLE OF ABERRANT Ig GLYCOSYLATION
    Jiri Mestecky; Fiscal Year: 2006
    ..Results of these studies will generate information concerning previously unexplored inflammatory pathways that participate in the development of PD. ..
  50. STRUCTURE/FUNCTION ANALYSIS OF THE UROKINASE RECEPTOR
    Douglas Cines; Fiscal Year: 2002
    ..abstract_text> ..
  51. Development of a Novel Method for Inhibiting Atherosclerosis in Diabetes
    David Clemmons; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  52. Glycosylation and memory T cell differentiation
    Thandi Onami; Fiscal Year: 2006
    ..abstract_text> ..
  53. 'Lyme disease: A possible test for cure
    MARIO PHILIPP; Fiscal Year: 2003
    ..Cure of LD will be assessed objectively (by culture and PCR) both in rhesus monkeys (chronic LD) and in mice (acute LD). Correlation between LD cure and anti-C6 Ab titers will be evaluated. ..
  54. Innate-like hepatic CD1d-reative NKT cells:Liver Disease
    Mark Exley; Fiscal Year: 2008
    ..I. on acute HCV infection in chimpanzees, as well as on liver immunology and on 'NKT' cells in general. ..
  55. Immunoglobulin Allotypes in Hepatitis C Virus Infection
    JANARDAN PANDEY; Fiscal Year: 2008
    ..Results of this investigation will advance our understanding of the role of host genetic factors in clearance and persistence of hepatitis C virus infection. ..
  56. Studies of Human and Murine SLE
    Betty Diamond; Fiscal Year: 2006
    ..This effort is based on already existing interactions among the investigators and will be substantially strengthened by the resources provided by the Program Project...
  57. MICELLAR CARRIERS FOR SPARINGLY SOLUBLE PHARMACEUTICALS
    Vladimir Torchilin; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  58. Regulation and Cell Biology of Beta-Secretase
    Kumar Sambamurti; Fiscal Year: 2008
    ..In aim 3, the basis of enhanced BACE activity in the complex will be evaluated. [unreadable] [unreadable]..
  59. Human monoclonal panel mimicking anthrax immune globulin
    Donald Reason; Fiscal Year: 2005
    ..The PA-specific panel we construct will be molecularly defined and characterized, incorporate the advantages of toxin-specific, polyclonal human sera, and lack the inherent risk associated with blood derived products. ..