Summary: The origin of life. It includes studies of the potential basis for life in organic compounds but excludes studies of the development of altered forms of life through mutation and natural selection, which is EVOLUTION.

Top Publications

  1. pmc Template-directed synthesis of a genetic polymer in a model protocell
    Sheref S Mansy
    Howard Hughes Medical Institute, Department of Molecular Biology and the Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Nature 454:122-5. 2008
  2. doi Ribosome biogenesis and control of cell proliferation: p53 is not alone
    Giulio Donati
    Department of Experimental Pathology, Alma Mater Studiorum, Universita di Bologna, Bologna, Italy
    Cancer Res 72:1602-7. 2012
  3. doi Spontaneous network formation among cooperative RNA replicators
    Nilesh Vaidya
    Department of Chemistry, Portland State University, PO Box 751, Portland, Oregon 97207, USA
    Nature 491:72-7. 2012
  4. doi Growth control and ribosome biogenesis
    Harri Lempiäinen
    Department of Molecular Biology and NCCR Program Frontiers in Genetics, University of Geneva, 30 Quai Ernest Ansermet, Geneva 4, Switzerland
    Curr Opin Cell Biol 21:855-63. 2009
  5. doi Lysosome biogenesis and lysosomal membrane proteins: trafficking meets function
    Paul Saftig
    Department of Biochemistry, Christian Albrechts University, Kiel, Germany
    Nat Rev Mol Cell Biol 10:623-35. 2009
  6. pmc Trex1 regulates lysosomal biogenesis and interferon-independent activation of antiviral genes
    Maroof Hasan
    Department of Internal Medicine, UT Southwestern Medical Center, Dallas, Texas, USA
    Nat Immunol 14:61-71. 2013
  7. doi Role of pri-miRNA tertiary structure in miR-17~92 miRNA biogenesis
    Steven G Chaulk
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    RNA Biol 8:1105-14. 2011
  8. doi Transcriptional and post-transcriptional regulation of mitochondrial biogenesis in skeletal muscle: effects of exercise and aging
    Vladimir Ljubicic
    School of Kinesiology and Health Science, York University, Toronto, Ontario, Canada M3J 1P3
    Biochim Biophys Acta 1800:223-34. 2010
  9. doi A hierarchical model for evolution of 23S ribosomal RNA
    Konstantin Bokov
    Departement de Biochimie, Universite de Montreal, Montreal, H3C 3J7 Quebec, Canada
    Nature 457:977-80. 2009
  10. pmc Mechanisms of control of microRNA biogenesis
    Brandi N Davis-Dusenbery
    Molecular Cardiology Research Institute, Tufts Medical Center, Boston, MA 02111, USA
    J Biochem 148:381-92. 2010

Research Grants

Detail Information

Publications350 found, 100 shown here

  1. pmc Template-directed synthesis of a genetic polymer in a model protocell
    Sheref S Mansy
    Howard Hughes Medical Institute, Department of Molecular Biology and the Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Nature 454:122-5. 2008
  2. doi Ribosome biogenesis and control of cell proliferation: p53 is not alone
    Giulio Donati
    Department of Experimental Pathology, Alma Mater Studiorum, Universita di Bologna, Bologna, Italy
    Cancer Res 72:1602-7. 2012
    Cell growth is a prerequisite for cell proliferation, and ribosome biogenesis is a limiting factor for cell growth...
  3. doi Spontaneous network formation among cooperative RNA replicators
    Nilesh Vaidya
    Department of Chemistry, Portland State University, PO Box 751, Portland, Oregon 97207, USA
    Nature 491:72-7. 2012
    ..We can observe the evolvability of networks through in vitro selection. Our experiments highlight the advantages of cooperative behaviour even at the molecular stages of nascent life...
  4. doi Growth control and ribosome biogenesis
    Harri Lempiäinen
    Department of Molecular Biology and NCCR Program Frontiers in Genetics, University of Geneva, 30 Quai Ernest Ansermet, Geneva 4, Switzerland
    Curr Opin Cell Biol 21:855-63. 2009
    ..and in higher eukaryotes, are beginning to reveal new mechanisms underlying the elaborate control of ribosome biogenesis, which requires coordinate regulation of all three RNA polymerases...
  5. doi Lysosome biogenesis and lysosomal membrane proteins: trafficking meets function
    Paul Saftig
    Department of Biochemistry, Christian Albrechts University, Kiel, Germany
    Nat Rev Mol Cell Biol 10:623-35. 2009
    ..lysosomal trafficking pathways is emphasized by recent findings that reveal new roles for lysosomal membrane proteins in cellular physiology and in an increasing number of diseases that are characterized by defects in lysosome biogenesis.
  6. pmc Trex1 regulates lysosomal biogenesis and interferon-independent activation of antiviral genes
    Maroof Hasan
    Department of Internal Medicine, UT Southwestern Medical Center, Dallas, Texas, USA
    Nat Immunol 14:61-71. 2013
    ..Together our data identify Trex1 as a regulator of lysosomal biogenesis and interferon-independent activation of antiviral genes and show that dysregulation of lysosomes can elicit ..
  7. doi Role of pri-miRNA tertiary structure in miR-17~92 miRNA biogenesis
    Steven G Chaulk
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    RNA Biol 8:1105-14. 2011
    ..To date, the role of RNA structure in miRNA biogenesis has only been considered in terms of the secondary structural elements required for processing of pri-miRNAs by ..
  8. doi Transcriptional and post-transcriptional regulation of mitochondrial biogenesis in skeletal muscle: effects of exercise and aging
    Vladimir Ljubicic
    School of Kinesiology and Health Science, York University, Toronto, Ontario, Canada M3J 1P3
    Biochim Biophys Acta 1800:223-34. 2010
    ..This review summarizes our current understanding of the effects of exercise on the gene expression pathway leading to organelle biogenesis in muscle.
  9. doi A hierarchical model for evolution of 23S ribosomal RNA
    Konstantin Bokov
    Departement de Biochimie, Universite de Montreal, Montreal, H3C 3J7 Quebec, Canada
    Nature 457:977-80. 2009
    ..Surprisingly, a detailed analysis of the structure of the modern ribosome reveals a concerted and modular scheme of its early evolution...
  10. pmc Mechanisms of control of microRNA biogenesis
    Brandi N Davis-Dusenbery
    Molecular Cardiology Research Institute, Tufts Medical Center, Boston, MA 02111, USA
    J Biochem 148:381-92. 2010
    ..The recent identification of mechanisms of miRNA biogenesis regulation uncovers that various factors or growth factor signalling pathways control every step of the miRNA ..
  11. pmc Plant peroxisomes: biogenesis and function
    Jianping Hu
    Michigan State University Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824, USA
    Plant Cell 24:2279-303. 2012
    ..Considerable progress has been made in the identification of factors involved in peroxisomal biogenesis, revealing mechanisms that are both shared with and diverged from non-plant systems...
  12. pmc Origin of first cells at terrestrial, anoxic geothermal fields
    Armen Y Mulkidjanian
    School of Physics, University of Osnabruck, D 49069 Osnabruck, Germany
    Proc Natl Acad Sci U S A 109:E821-30. 2012
  13. pmc Serpentinite and the dawn of life
    Norman H Sleep
    Department of Geophysics, Stanford University, Stanford, CA 94305, USA
    Philos Trans R Soc Lond B Biol Sci 366:2857-69. 2011
    ..9 Ga, but older rocks that might retain evidence of its presence have not been found. Earth's sequesters extensive evidence of Archaean and younger subducted biological material, but has yet to be exploited for the Hadean record...
  14. doi Comparison of the roles of nucleotide synthesis, polymerization, and recombination in the origin of autocatalytic sets of RNAs
    Meng Wu
    Origins Institute and Department of Physics and Astronomy, McMaster University, Hamilton, Ontario, Canada
    Astrobiology 11:895-906. 2011
  15. pmc Computational analysis of mouse piRNA sequence and biogenesis
    Doron Betel
    Computational and Systems Biology Center, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America
    PLoS Comput Biol 3:e222. 2007
    ..microRNAs and repeat-associated small interfering RNAs (rasiRNAs), has raised puzzling questions regarding their biogenesis and function...
  16. doi Mechanisms of autophagosome biogenesis
    David C Rubinsztein
    Department of Medical Genetics, Cambridge Institute for Medical Research, Wellcome MRC Building, Addenbrooke s Hospital, Cambridge CB2 0XY, UK
    Curr Biol 22:R29-34. 2012
    ..studies in yeast and mammalian systems that have provided insights into two critical areas of autophagosome biogenesis - the source of the autophagosomal membranes, and the mechanisms regulating the fusion of the edges of the ..
  17. doi Role of MINOS in mitochondrial membrane architecture and biogenesis
    Martin van der Laan
    Institut fur Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Universitat Freiburg, 79104 Freiburg, Germany
    Trends Cell Biol 22:185-92. 2012
    ..Here, we propose that MINOS forms a central part of an ER-mitochondria organizing network (ERMIONE) that controls mitochondrial membrane architecture and biogenesis.
  18. doi Glycogen synthase kinase-3 inhibition reduces ischemic cerebral damage, restores impaired mitochondrial biogenesis and prevents ROS production
    Alessandra Valerio
    Division of Pharmacology, Department of Biomedical Sciences and Biotechnology, University of Brescia, Brescia, Italy
    J Neurochem 116:1148-59. 2011
    This study was designed to test the hypothesis that improved mitochondrial biogenesis could help reducing ischemic cerebral injury...
  19. ncbi Biogenesis of small nuclear RNPs
    Tamas Kiss
    Laboratoire de Biologie Moleculaire Eucaryote du CNRS, UMR5099, Universite Paul Sabatier, IFR109, 118 route de Narbonne, 31062 Toulouse Cedex 4, France
    J Cell Sci 117:5949-51. 2004
  20. ncbi The antiquity of RNA-based evolution
    Gerald F Joyce
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Nature 418:214-21. 2002
    ..This earlier era is referred to as the 'RNA world', during which the genetic information resided in the sequence of RNA molecules and the phenotype derived from the catalytic properties of RNA...
  21. pmc Regulation of microRNA biogenesis and turnover by animals and their viruses
    Valentina Libri
    Centre for Immunity, Infection and Evolution, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh, EH9 3JT, UK
    Cell Mol Life Sci 70:3525-44. 2013
    ..of the pathways by which miRNAs are produced, matured and turned-over has revealed many aspects of their biogenesis that are subject to regulation...
  22. ncbi Secretory granule biogenesis: rafting to the SNARE
    S A Tooze
    Secretory Pathways Laboratory, 44 Lincoln s Inn Fields, London, UK WC2A 3PX
    Trends Cell Biol 11:116-22. 2001
    ..At the heart of these findings is the elucidation of molecular mechanisms driving protein--lipid and protein--protein interactions specific for secretory granule biogenesis.
  23. pmc Evolution of functional nucleic acids in the presence of nonheritable backbone heterogeneity
    Simon G Trevino
    Howard Hughes Medical Institute, Center for Computational and Integrative Biology, and Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02144, USA
    Proc Natl Acad Sci U S A 108:13492-7. 2011
    ..We conclude that nonheritable variations in nucleic acid backbone structure may not have posed an insurmountable barrier to the emergence of functionality in early nucleic acids...
  24. pmc Bioenergetics and life's origins
    David Deamer
    Department of Biomolecular Engineering, Baskin School of Engineering, University of California, Santa Cruz, California 95064, USA
    Cold Spring Harb Perspect Biol 2:a004929. 2010
    ..This article examines potential sources of energy available to protocells, and mechanisms by which the energy could be used to drive polymer synthesis...
  25. doi Chemical evolution from simple inorganic compounds to chiral peptides
    Thomas A E Jakschitz
    Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck, Innrain 80 82, 6020 Innsbruck, Austria
    Chem Soc Rev 41:5484-9. 2012
    ..Furthermore it proposes different answers to one of the big secrets of nature: why DNA-coded biohomochiral life emerged using amino acids in their l-form?..
  26. ncbi The roads to and from the RNA world
    Jason P Dworkin
    Laboratory for Extraterrestrial Physics, Code 691, NASA Goddard Space Flight Center, Greenbelt, MD 20771, USA
    J Theor Biol 222:127-34. 2003
    ..The possibility that DNA may have come before RNA is discussed, although it is a less parsimonious explanation than DNA following RNA...
  27. doi Out of fuzzy chemistry: from prebiotic chemistry to metabolic networks
    Juli Peretó
    Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Departament de Bioquimica i Biologia Molecular, Universitat de Valencia, C Jose Beltran 2, 46980 Paterna, Spain
    Chem Soc Rev 41:5394-403. 2012
    ..I suggest that a leitmotif in the origin and evolution of metabolism is the role played by catalysers' substrate ambiguity and multifunctionality...
  28. pmc The dawn of the RNA World: toward functional complexity through ligation of random RNA oligomers
    Carlos Briones
    Centro de Astrobiologia CSIC INTA, 28850 Torrejon de Ardoz, Madrid, Spain
    RNA 15:743-9. 2009
    ..This allows us to build up a stepwise model of ligation-based, modular evolution that could pave the way to the emergence of a ribozyme with RNA replicase activity, step at which information-driven Darwinian evolution would be triggered...
  29. pmc Originator dynamics
    Michael Manapat
    Program for Evolutionary Dynamics, Harvard University, Cambridge, MA 02138, USA
    J Theor Biol 256:586-95. 2009
    ..We study these reversals, which mark the transition from prelife to life in our model. If the replication potential exceeds a critical value, then life replicates into existence...
  30. pmc Mineral surfaces, geochemical complexities, and the origins of life
    Robert M Hazen
    Geophysical Laboratory, Carnegie Institution of Washington, Washington, DC 20015, USA
    Cold Spring Harb Perspect Biol 2:a002162. 2010
    ..Thus, mineral surfaces may have contributed centrally to the linked prebiotic problems of containment and organization by promoting the transition from a dilute prebiotic "soup" to highly ordered local domains of key biomolecules...
  31. ncbi Gender dimorphism in rat liver mitochondrial oxidative metabolism and biogenesis
    Roberto Justo
    Grup de Metabolisme Energetic i Nutricio, Departament de Biologia Fonamental i Ciencies de la Salut, Institut Universitari d Investigacio en Ciencies de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Spain
    Am J Physiol Cell Physiol 289:C372-8. 2005
  32. doi Non-enzymatic transfer of sequence information under plausible prebiotic conditions
    Felix Olasagasti
    Department of Biomolecular Engineering, Baskin School of Engineering, University of California, Santa Cruz, CA 95060, USA
    Biochimie 93:556-61. 2011
    ..9%. We present these results as a proof of concept for the possibility of having non-enzymatic transfer of sequence information in a prebiotically plausible environment...
  33. ncbi Lipid-assisted synthesis of RNA-like polymers from mononucleotides
    Sudha Rajamani
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    Orig Life Evol Biosph 38:57-74. 2008
    ..In the final hydration step, the RNA-like polymer is encapsulated within lipid vesicles. This process provides a laboratory model of an early stage of evolution toward an RNA World...
  34. ncbi Ribozyme catalysis of metabolism in the RNA world
    Xi Chen
    Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    Chem Biodivers 4:633-55. 2007
  35. ncbi The first steps of chemical evolution towards the origin of life
    Bernd M Rode
    Institute for General, Inorganic, and Theoretical Chemistry, University of Innsbruck, Innrain 52a, A 6020 Innsbruck
    Chem Biodivers 4:2674-702. 2007
  36. pmc Activation of mitochondrial biogenesis by heme oxygenase-1-mediated NF-E2-related factor-2 induction rescues mice from lethal Staphylococcus aureus sepsis
    Nancy Chou Macgarvey
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Am J Respir Crit Care Med 185:851-61. 2012
    ..control is regulated in part by the heme oxygenase-1 (HO-1; Hmox1) system through the redox-regulated NF-E2-related factor-2 (Nrf2) transcription factor, but its role in mitochondrial biogenesis in Staphylococcus aureus sepsis is unknown.
  37. pmc Impaired mitochondrial biogenesis contributes to mitochondrial dysfunction in Alzheimer's disease
    Baiyang Sheng
    Department of Pathology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Neurochem 120:419-29. 2012
    ..In the current study, we investigated whether alterations in mitochondrial biogenesis contribute to mitochondrial abnormalities in AD...
  38. ncbi Coordinated changes in mitochondrial function and biogenesis in healthy and diseased human skeletal muscle
    Anne Garnier
    Cardiologie Cellulaire et Moléculaire U 446 INSERM, Chatenay Malabry, France
    FASEB J 19:43-52. 2005
    We examined the transcriptional signaling cascade involved in the changes of mitochondrial biogenesis and mitochondrial function of skeletal muscle and of the exercise capacity of humans in response to long-term physical activity and ..
  39. pmc Regulation of mitochondrial biogenesis
    Francois R Jornayvaz
    Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06536, USA
    Essays Biochem 47:69-84. 2010
    ..receptor gamma co-activator-1alpha) is a co-transcriptional regulation factor that induces mitochondrial biogenesis by activating different transcription factors, including nuclear respiratory factor 1 and nuclear respiratory ..
  40. ncbi Rosiglitazone induces mitochondrial biogenesis in mouse brain
    Jay C Strum
    Department of Quantitative Expression, Division of Genetics Research, GlaxoSmithKline, Research Triangle Park, NC 27278, USA
    J Alzheimers Dis 11:45-51. 2007
    Rosiglitazone was found to simulate mitochondrial biogenesis in mouse brain in an apolipoprotein (Apo) E isozyme-independent manner...
  41. ncbi The stepwise evolution of early life driven by energy conservation
    James G Ferry
    Department of Biochemistry and Molecular Biology and Pennsylvania State Astrobiology Research Center, 205 South Frear Laboratory, Pennsylvania State University, USA
    Mol Biol Evol 23:1286-92. 2006
    ..We further propose that energy conservation played the predominant role in the later evolution of anaerobic metabolisms which explains the origin and evolution of extant methanogenic pathways...
  42. doi Origin of self-replicating biopolymers: autocatalytic feedback can jump-start the RNA world
    Meng Wu
    Department of Physics and Astronomy, Origins Institute, McMaster University, Hamilton, ON, L8S 4M1, Canada
    J Mol Evol 69:541-54. 2009
    ..This transition occurs most easily in volumes of intermediate size...
  43. ncbi Biogenesis and germline functions of piRNAs
    Carla Klattenhoff
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Development 135:3-9. 2008
    ..organization, gene transcription, RNA stability or RNA translation is not well understood, neither is piRNA biogenesis. Here, we review recent studies of piRNA production and function, and discuss unanswered questions about this ..
  44. pmc Prevolutionary dynamics and the origin of evolution
    Martin A Nowak
    Program for Evolutionary Dynamics, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 105:14924-7. 2008
    ..Replication is not a prerequisite for selection, but instead, there can be selection for replication. Mutation leads to an error threshold between life and prelife...
  45. pmc Cell-cell contact globally activates microRNA biogenesis
    Hun Way Hwang
    Program in Human Genetics and Molecular Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 106:7016-21. 2009
    ..The molecular mechanisms that govern the global efficiency of miRNA biogenesis in these settings remain incompletely understood, and experimental systems for the biochemical dissection of ..
  46. ncbi The habitat and nature of early life
    E G Nisbet
    Department of Geology, University of London, Egham, UK
    Nature 409:1083-91. 2001
    ..By about 3,500 million years ago, most of the principal biochemical pathways that sustain the modern biosphere had evolved, and were global in scope...
  47. doi Fructose 1,6-bisphosphate aldolase/phosphatase may be an ancestral gluconeogenic enzyme
    Rafael F Say
    Mikrobiologie, Fakultat Biologie, Universitat Freiburg, Schanzlestrasse 1, D 79104 Freiburg, Germany
    Nature 464:1077-81. 2010
    ..We propose that this highly conserved, heat-stabile and bifunctional FBP aldolase/phosphatase represents the pace-making ancestral gluconeogenic enzyme, and that in evolution gluconeogenesis preceded glycolysis...
  48. pmc Phosphatidylinositol 3-monophosphate is involved in toxoplasma apicoplast biogenesis
    Lina Tawk
    UMR 5235 CNRS, Universite Montpellier 1 and 2, Montpellier, France
    PLoS Pathog 7:e1001286. 2011
    ..Here we show that the lipid phosphatidylinositol 3-monophosphate (PI3P) is involved in apicoplast biogenesis in Toxoplasma gondii...
  49. doi How did LUCA make a living? Chemiosmosis in the origin of life
    Nick Lane
    Department of Genetics, Evolution and Environment, University College London, London, UK
    Bioessays 32:271-80. 2010
    ..Synthesis of ATP by chemiosmosis today involves generation of an ion gradient by means of vectorial electron transfer from a donor to an acceptor. We argue that the first donor was hydrogen and the first acceptor CO2...
  50. ncbi Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes
    An Chi
    Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904, USA
    J Proteome Res 5:3135-44. 2006
    ..The sharing of proteins between melanosomes and other lysosome-related organelles suggests a common evolutionary origin. This work represents a model for the study of the biogenesis of lysosome-related organelles.
  51. pmc Evolution before genes
    Vera Vasas
    Departament de Genetica i de Microbiologia, Grup de Biologia Evolutiva GBE, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
    Biol Direct 7:1; discussion 1. 2012
    ..However, this is exactly the kind of scheme we propose for how Darwinian evolution could have occurred prior to template replication...
  52. pmc ADAMTSL4, a secreted glycoprotein widely distributed in the eye, binds fibrillin-1 microfibrils and accelerates microfibril biogenesis
    Luis A R Gabriel
    Departments of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Invest Ophthalmol Vis Sci 53:461-9. 2012
    ..The authors sought to characterize recombinant ADAMTSL4 and the ocular distribution of ADAMTSL4 and to investigate whether ADAMTSL4 influences the biogenesis of fibrillin-1 microfibrils, which compose the zonule.
  53. doi PGC-1 beta-regulated mitochondrial biogenesis and function in myotubes is mediated by NRF-1 and ERR alpha
    Di Shao
    The National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China
    Mitochondrion 10:516-27. 2010
    ..ablated the aforesaid function of PGC-1 beta and compromised the oxidative phosphorylation and mitochondrial biogenesis. Taken together, these results confirm the direct interaction of NRF-1 and ERR alpha with PGC-1 beta, and their ..
  54. doi Possible origin of life between mica sheets
    Helen Greenwood Hansma
    Department of Physics, University of California, Santa Barbara, CA 93106, USA
    J Theor Biol 266:175-88. 2010
    ..Therefore the mica hypothesis has the potential to unify origins hypotheses, such that different molecular components and systems could simultaneously evolve in the spaces between mica sheets...
  55. pmc Biogenesis of the Saccharomyces cerevisiae pheromone a-factor, from yeast mating to human disease
    Susan Michaelis
    Department of Cell Biology, The Johns Hopkins School of Medicine, Baltimore, Maryland, USA
    Microbiol Mol Biol Rev 76:626-51. 2012
    ..In this review, we discuss the three modules comprising the a-factor biogenesis pathway: (i) the C-terminal CAAX-processing steps carried out by Ram1/Ram2, Ste24 or Rce1, and Ste14; (ii) two ..
  56. doi Seeking the chemical roots of darwinism: bridging between chemistry and biology
    Addy Pross
    Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva, 84105, Israel
    Chemistry 15:8374-81. 2009
    ..Possible implications of these ideas to the origin of life problem and the relationship between chemical emergence and biological evolution are discussed...
  57. pmc Evolutionary dynamics of RNA-like replicator systems: A bioinformatic approach to the origin of life
    Nobuto Takeuchi
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, 8600 Rockville Pike, Bethesda, MD 20894, USA
    Phys Life Rev 9:219-63. 2012
    ..In conclusion, we pose a future question for theoretical studies on the origin of life...
  58. pmc Self-assembly of VPS41 promotes sorting required for biogenesis of the regulated secretory pathway
    Cédric S Asensio
    Department of Physiology, University of California, San Francisco, San Francisco, CA 94158, USA Department of Neurology, University of California, San Francisco, San Francisco, CA 94158, USA
    Dev Cell 27:425-37. 2013
    ..VPS41 also self-assembles into a lattice, suggesting that it acts as a coat protein for AP-3 in formation of the regulated secretory pathway. ..
  59. doi Resistance exercise enhances the molecular signaling of mitochondrial biogenesis induced by endurance exercise in human skeletal muscle
    Li Wang
    The Astrand Laboratory of Work Physiology, GIH, The Swedish School of Sport and Health Sciences, Box 5626, 114 86 Stockholm, Sweden
    J Appl Physiol (1985) 111:1335-44. 2011
    ..We have investigated the hypothesis that molecular signaling of mitochondrial biogenesis after endurance exercise is impaired by resistance exercise...
  60. ncbi Increased mitochondrial biogenesis in primary leukemia cells: the role of endogenous nitric oxide and impact on sensitivity to fludarabine
    J S Carew
    Department of Molecular Pathology, The University of Texas, MD Anderson Cancer Center, Houston, TX 77030, USA
    Leukemia 18:1934-40. 2004
    ..Considering that NO levels can affect mitochondrial biogenesis, we hypothesized that the inherent nitrosative stress in CLL cells may lead to hyperactive mitochondrial ..
  61. pmc Biogenesis of cytoplasmic membranous vesicles for plant potyvirus replication occurs at endoplasmic reticulum exit sites in a COPI- and COPII-dependent manner
    Taiyun Wei
    Southern Crop Protection and Food Research Centre, AAFC, 1391 Sandford Street, London, Ontario N5V 4T3, Canada
    J Virol 82:12252-64. 2008
    Single-stranded positive-sense RNA viruses induce the biogenesis of cytoplasmic membranous vesicles, where viral replication takes place...
  62. doi Probing teichoic acid genetics with bioactive molecules reveals new interactions among diverse processes in bacterial cell wall biogenesis
    Michael A D'Elia
    Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada
    Chem Biol 16:548-56. 2009
    ..Furthermore, the new reporter assay represents an exciting avenue for the discovery of antibacterial molecules...
  63. ncbi Monte Carlo simulation of early molecular evolution in the RNA World
    Wentao Ma
    College of Life Sciences, Wuhan University, Wuhan 430072, PR China
    Biosystems 90:28-39. 2007
  64. doi ArfGAP1 activity and COPI vesicle biogenesis
    Rainer Beck
    Heidelberg University Biochemistry Center, Heidelberg University, Im Neuenheimer Feld 328, Heidelberg, Germany
    Traffic 10:307-15. 2009
    ..Various and opposing reports exist on a role of ArfGAP1 in COPI vesicle biogenesis. In this study, we show that, in contrast to data in the literature, ArfGAP1 is not required for COPI vesicle ..
  65. pmc Age associated low mitochondrial biogenesis may be explained by lack of response of PGC-1α to exercise training
    Frederic Derbré
    Laboratory Movement Sport and Health Sciences, University Rennes, France
    Age (Dordr) 34:669-79. 2012
    ..To sum up, the low mitochondrial biogenesis associated with aging may be due to the lack of response of PGC-1α to different stimuli...
  66. ncbi Detecting autocatalytic, self-sustaining sets in chemical reaction systems
    Wim Hordijk
    Biomathematics Research Centre, The University of Canterbury, Room 623, Private Bag 4800, Christchurch, New Zealand
    J Theor Biol 227:451-61. 2004
    ..Using simulations and some analytic techniques we investigate the rate of catalysis that is required for the emergence of autocatalytic and sustaining subsystems...
  67. doi A formal test of the theory of universal common ancestry
    Douglas L Theobald
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 01778, USA
    Nature 465:219-22. 2010
    ..These results provide powerful statistical evidence corroborating the monophyly of all known life...
  68. ncbi The origin of autonomous agents by natural selection
    Chrisantha Fernando
    School of Computer Science, University of Birmingham, Birmingham B15 2TT, UK
    Biosystems 91:355-73. 2008
    ..This model evolved increasingly complex self-sustaining processes of constitution, a result that is also expected in real chemistry...
  69. pmc Primordial soup or vinaigrette: did the RNA world evolve at acidic pH?
    Harold S Bernhardt
    Department of Biochemistry, University of Otago, Dunedin, New Zealand
    Biol Direct 7:4. 2012
    ..As the Hadean Eon progressed, the ocean pH is inferred to have gradually risen to near neutral as atmospheric CO(2) levels decreased...
  70. pmc NBEAL2 is mutated in gray platelet syndrome and is required for biogenesis of platelet α-granules
    Meral Gunay-Aygun
    Section on Human Biochemical Genetics, Medical Genetics Branch, National Human Genome Research Institute, US National Institutes of Health, Bethesda, Maryland, USA
    Nat Genet 43:732-4. 2011
    ..Proteomic analysis of sucrose-gradient subcellular fractions of platelets indicated that NBEAL2 localizes to the dense tubular system (endoplasmic reticulum) in platelets...
  71. doi Pumice as a remarkable substrate for the origin of life
    Martin D Brasier
    Department of Earth Sciences, University of Oxford, Oxford, UK
    Astrobiology 11:725-35. 2011
    ..These remarkable properties now deserve to be rigorously explored in the laboratory and the early rock record...
  72. doi Primordial ocean chemistry and its compatibility with the RNA world
    Jeremy Kua
    Department of Chemistry and Biochemistry, University of San Diego, 5998 Alcala Park, San Diego, CA 92110, USA
    Orig Life Evol Biosph 41:553-8. 2011
    ..The conditions we have chosen range from highly acidic or alkaline hydrothermal vents, to the milder conditions in a primordial ocean at a range of atmospheric CO(2) partial pressures...
  73. pmc Lethal mutants and truncated selection together solve a paradox of the origin of life
    David B Saakian
    Institute of Physics, Academia Sinica, Nankang, Taipei, Taiwan
    PLoS ONE 6:e21904. 2011
    ..This is the famous error catastrophe paradox. How to solve this puzzle is an interesting and important problem in the theory of the origin of life...
  74. pmc Closing the circle: replicating RNA with RNA
    Leslie K L Cheng
    Simon Fraser University, 8888 University Drive, Burnaby, BC V5A 1S6, Canada
    Cold Spring Harb Perspect Biol 2:a002204. 2010
    ..Recent progress into the selection and characterization of ribozymes that promote nucleotide synthesis and RNA polymerization are discussed and outstanding problems in the field of RNA-mediated RNA replication are summarized...
  75. ncbi Testing fundamental evolutionary hypotheses
    David Penny
    Allan Wilson Center for Molecular Ecology and Evolution, Massey University, Palmerston North, New Zealand
    J Theor Biol 223:377-85. 2003
    ..The uniqueness (or not) of the origin of life, though still difficult, is similarly amenable to the testing of alternative hypotheses...
  76. ncbi Emergence of information transmission in a prebiotic RNA reactor
    Benedikt Obermayer
    Arnold Sommerfeld Center für Theoretische Physik and Center for NanoScience, Ludwig Maximilians Universitat Munchen, Germany
    Phys Rev Lett 107:018101. 2011
    ..By analyzing stochastic simulations, we find temporal sequence correlations that constitute a signature of information transmission, weaker but of the same form as in a true replicator...
  77. ncbi Origins of life: How leaky were primitive cells?
    David W Deamer
    Nature 454:37-8. 2008
  78. doi Early targeting events during membrane protein biogenesis in Escherichia coli
    Eitan Bibi
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    Biochim Biophys Acta 1808:841-50. 2011
    ..This article is part of a Special Issue entitled Protein translocation across or insertion into membranes...
  79. pmc Mutation of a gene essential for ribosome biogenesis, EMG1, causes Bowen-Conradi syndrome
    Joy Armistead
    Department of Biochemistry and Medical Genetics, University of Manitoba, MB, Canada
    Am J Hum Genet 84:728-39. 2009
    ..These findings suggested that the D-to-G mutation caused aggregation of EMG1, thereby reducing the level of the protein and causing BCS...
  80. doi Biogenesis of sperm perinuclear theca and its role in sperm functional competence and fertilization
    Richard Oko
    Department of Anatomy and Cell Biology, Queen s University, 9th Floor, Botterell Hall, Kingston, ON, Canada
    J Reprod Immunol 83:2-7. 2009
    ..In this review data collected on resident perinuclear theca proteins, SubH2Bv, RAB2, PAWP and the four core somatic histones, is used to substantiate these hypotheses...
  81. ncbi Characters of very ancient proteins
    Bin Guang Ma
    Shandong Provincial Research Center for Bioinformatic Engineering and Technique, Center for Advanced Study, Shandong University of Technology, Zibo 255049, PR China
    Biochem Biophys Res Commun 366:607-11. 2008
  82. doi Serpentinization as a source of energy at the origin of life
    M J Russell
    Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, USA
    Geobiology 8:355-71. 2010
  83. doi Hydrothermal focusing of chemical and chemiosmotic energy, supported by delivery of catalytic Fe, Ni, Mo/W, Co, S and Se, forced life to emerge
    Wolfgang Nitschke
    Laboratoire de Bioénergétique et Ingénierie des Protéines CNRS UPR9036, IFR88, 31 Chemin Joseph Aiguier, 13402, Marseille Cedex 20, France
    J Mol Evol 69:481-96. 2009
    ..Thus the two prokaryotic domains both emerged within the hydrothermal mound-the acetogens were the forerunners of the Bacteria and the methanogens were the forerunners of the Archaea...
  84. pmc Distribution patterns of small-molecule ligands in the protein universe and implications for origin of life and drug discovery
    Hong Fang Ji
    Shandong Provincial Research Center for Bioinformatic Engineering and Technique, Center for Advanced Study, Shandong University of Technology, Zibo 255049, PR China
    Genome Biol 8:R176. 2007
    ..Extant life depends greatly on the binding of small molecules (such as ligands) with macromolecules (such as proteins), and one ligand can bind multiple proteins. However, little is known about the global patterns of ligand-protein mapping...
  85. doi Biochemical and structural insights into bacterial organelle form and biogenesis
    Joshua B Parsons
    Protein Science Group, Department of Biochemistry, University of Kent, Canterbury, Kent, UK
    J Biol Chem 283:14366-75. 2008
    ..The recombinant model will facilitate future experiments establishing the structure and assembly of these multiprotein assemblages and their fate when the specific metabolic function is no longer required...
  86. doi Evolutionary analysis and molecular dissection of caveola biogenesis
    Matthew Kirkham
    Institute for Molecular Bioscience, University of Queensland, Queensland, Brisbane, Australia
    J Cell Sci 121:2075-86. 2008
    ..However, coexpression of flotillin-1 and flotillin-2 did not cause caveola biogenesis in this system. In contrast to the other tested caveolins, C...
  87. doi Biogenesis, regulation, and targeting of the type III secretion system
    Thierry Izoré
    Bacterial Pathogenesis Group, Institut de Biologie Structurale, Universite Grenoble I, 38027 Grenoble, France
    Structure 19:603-12. 2011
    ..The biogenesis of this complex machinery requires the regulated interaction between over 20 cytosolic, periplasmic, and ..
  88. pmc On the origins of cells: a hypothesis for the evolutionary transitions from abiotic geochemistry to chemoautotrophic prokaryotes, and from prokaryotes to nucleated cells
    William Martin
    Institut für Botanik III, Heinrich Heine Universitaet Düsseldorf, Universitatsstrasse 1, 40225 Dusseldorf, Germany
    Philos Trans R Soc Lond B Biol Sci 358:59-83; discussion 83-5. 2003
    ..Under these premises, the most ancient divide in the living world is that between eubacteria and archaebacteria, yet the steepest evolutionary grade is that between prokaryotes and eukaryotes...
  89. pmc Expanding roles for diverse physical phenomena during the origin of life
    Itay Budin
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114 2696, USA
    Annu Rev Biophys 39:245-63. 2010
    ..In the absence of evolved biochemical capabilities, such physical processes likely played an essential role in early biology...
  90. pmc Coupled growth and division of model protocell membranes
    Ting F Zhu
    Howard Hughes Medical Institute, and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    J Am Chem Soc 131:5705-13. 2009
    ..In addition, the robustness and simplicity of this pathway suggests that similar processes might have occurred under the prebiotic conditions of the early Earth...
  91. ncbi Filamentous microfossils in a 3,235-million-year-old volcanogenic massive sulphide deposit
    B Rasmussen
    Department of Geology and Geophysics, University of Western Australia, Nedlands, Australia
    Nature 405:676-9. 2000
    ..Such environments may have hosted the first living systems on Earth, consistent with proposals for a thermophilic origin of life...
  92. doi A simple template-dependent ligase ribozyme as the RNA replicase emerging first in the RNA world
    Wentao Ma
    College of Life Sciences, Wuhan University, Wuhan, People s Republic of China
    Astrobiology 10:437-47. 2010
  93. ncbi Membrane self-assembly processes: steps toward the first cellular life
    Pierre Alain Monnard
    Department of Chemistry and Biochemistry, University of California Santa Cruz, USA
    Anat Rec 268:196-207. 2002
    ..Their suspensions in aqueous media have therefore been used to investigate nutrient uptake across simple membranes and encapsulated catalyzed reactions, both of which would be essential processes in protocellular life forms...
  94. ncbi From replicators to reproducers: the first major transitions leading to life
    E Szathmary
    Department of Plant Taxonomy and Ecology, Eotvos University, Budapest, Hungary
    J Theor Biol 187:555-71. 1997
    ..We outline Griesemer's concept of a reproducer, which brings out the essential role of development in evolution...
  95. pmc Enrichment of the amino acid L-isovaline by aqueous alteration on CI and CM meteorite parent bodies
    Daniel P Glavin
    National Aeronautics and Space Administration Goddard Space Flight Center, Greenbelt, MD 20771, USA
    Proc Natl Acad Sci U S A 106:5487-92. 2009
  96. pmc Fossil evidence of Archaean life
    J William Schopf
    University of California, Department of Earth and Space Sciences, Institute of Geophysics and Planetary Physics Center for the Study of Evolution and the Origin of Life, Los Angeles, CA 90095, USA
    Philos Trans R Soc Lond B Biol Sci 361:869-85. 2006
    ..These compilations support the view that life's existence dates from more than or equal to 3500 Myr ago...
  97. doi Synthesis of activated pyrimidine ribonucleotides in prebiotically plausible conditions
    Matthew W Powner
    School of Chemistry, The University of Manchester, Oxford Road, Manchester M13 9PL, UK
    Nature 459:239-42. 2009
    ..For prebiotic reaction sequences, our results highlight the importance of working with mixed chemical systems in which reactants for a particular reaction step can also control other steps...
  98. pmc A two-step chemical mechanism for ribosome-catalysed peptide bond formation
    David A Hiller
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Nature 476:236-9. 2011
    ..This suggests that in addition to substrate positioning, the ribosome is contributing to chemical catalysis by changing the rate-limiting transition state...
  99. ncbi Testing the hypothesis of common ancestry
    Elliott Sober
    Department of Philosophy, University of Wisconsin, Madison 53706, USA
    J Theor Biol 218:395-408. 2002
    ..We then describe some theoretical results that suggest the hypothesis may be intrinsically difficult to test. We conclude by suggesting an approach to the problem based on the Aikaike information criterion...
  100. ncbi In silico simulations reveal that replicators with limited dispersal evolve towards higher efficiency and fidelity
    Peter Szabo
    Department of Plant Taxonomy and Ecology, Research Group of Ecology and Theoretical Biology, Eotvos University, 1 C Pázmány P sétány, H 1117 Budapest, Hungary
    Nature 420:340-3. 2002
  101. ncbi An off-axis hydrothermal vent field near the Mid-Atlantic Ridge at 30 degrees N
    D S Kelley
    University of Washington, School of Oceanography, Seattle, Washington 98195, USA
    Nature 412:145-9. 2001

Research Grants81

  1. Functional Genomics of the Mitochondrial Proteome
    Dennis R Winge; Fiscal Year: 2011
    ..system for mitochondrial protein quality control and Coa4 which are novel assembly factors for the biogenesis of cytochrome c reductase and cytochrome oxidase, respectively...
  2. Role of the Mammalian Siderophore in Cellular Iron Metabolism and Erythropoiesis
    Laxminarayana R Devireddy; Fiscal Year: 2012
    ..We have also identified several novel genes that are necessary for the biogenesis of the mammalian siderophore...
  3. Iron in the pathogenesis of Friedreich's ataxia
    ..Its putative normal function is the biogenesis of iron- sulfur (Fe-S) clusters conveying iron homeostasis to the entire cell...
  4. Characterization of new toxins (YmgD and YdfD) from E.coli, targeting cell wall
    Hisako Masuda; Fiscal Year: 2012
    ..In this application, I will attempt to decipher the exact molecular reactions in peptidoglycan (PG) biogenesis, which these toxins target...
  5. Non-coding RNA engineers antibody diversity
    Uttiya Basu; Fiscal Year: 2011
    ..Implication: If successful, this study will be the first to demonstrate the role of ncRNA biogenesis pathway in the genetic and epigenetic control of the Ig locus...
  6. Role of GDNF in the regulation of hepatic steatosis
    SHANTHI K SRINIVASAN; Fiscal Year: 2013 hepatocytes, resulting in up regulation of enzymes related to fatty acids beta-oxidation and mitochondrial biogenesis. We will establish the necessity and sufficiency of GDNF in regulating hepatic fatty acid oxidation using mice ..
  7. Molecular Biology of Human Erythrocyte Alpha Spectrin
    Patrick G Gallagher; Fiscal Year: 2013
    ..Results obtained from these studies will allow study of the role of 1-spectrin in erythropoiesis, membrane biogenesis, and inherited erythrocyte disorders...
  8. Metabolic regulation of diabetic nephropathy
    Karen Block; Fiscal Year: 2013
    ..Reduced expression of mitochondrial genes involved in mitochondrial biogenesis is associated with altered cellular metabolism and oxygen consumption...
    L Jeannine Brady; Fiscal Year: 2013
    ..These objectives will advance our understanding of acid tolerance and surface biogenesis in S...
  10. Roles of RNA Polymerases IV and V in siRNA-mediated gene silencing
    CRAIG STUART PIKAARD; Fiscal Year: 2013
    ..Pol IV acts early in the pathway, generating transcripts that are required for the biogenesis of 24 nt short interfering RNAs (siRNAs) that are loaded into ARGONAUTE 4 (AGO4)...
  11. Cellular and Molecular Regulation of Thrombopoiesis
    Ramesh A Shivdasani; Fiscal Year: 2012
    ..Second, although NF-E2 is an essential effector of late MK maturation and platelet biogenesis, satisfactory understanding of its mechanisms and transcriptional targets has been elusive...
  12. Respiration in Sepsis
    Claude A Piantadosi; Fiscal Year: 2013
    ..renewal application proposes to study transcriptional regulation of the pro-survival program of mitochondrial biogenesis during experimental S. aureus sepsis...
  13. Oxidative Stress in Chronic Kidney Disease
    Talat Alp Ikizler; Fiscal Year: 2012
    ..and dietary caloric restriction, have demonstrated antioxidant effects mediated via induction of mitochondrial biogenesis, induction of antioxidant enzymes, and reduction of free radical mitochondrial leak during oxidative ..
  14. Adipose Tissue Macrophage Phenotype and Function
    Anthony W Ferrante; Fiscal Year: 2013
    ..rather then inducing a classical inflammatory program in ATMs activates a program of lipid uptake and lysosome biogenesis. From these data emerge a dichotomous picture of ATMs in which their action on adipocytes and adipose tissue ..
  15. Testing the efficacy of PGC-1alpha as a therapeutic target for Parkinson's diseas
    HANS BUELER; Fiscal Year: 2010
    ..coactivator-11 (PGC-1a) is a transcriptional coactivator of nuclear receptors that stimulates mitochondrial biogenesis and function...
  16. Characterization of an essential mitochondrial rRNA methyltransferase
    Kip E Guja; Fiscal Year: 2013
    ..A crucial requirement for mitochondrial gene expression is proper ribosome biogenesis. This process, which is not well understood, involves several posttranscriptional RNA modifications that are ..
  17. High-affinity RNA targets of Survival Motor Neuron Protein
    Ravindra N Singh; Fiscal Year: 2013
    ..Among several important functions, SMN has been implicated in biogenesis of small-nuclear ribonucleoproteins (snRNPs), transcription, pre-mRNA splicing, macromolecular trafficking, ..
    Frances M Brodsky; Fiscal Year: 2013
    ..CCVs implement the fundamental cellular membrane traffic pathways of endocytosis and lysosome biogenesis. CCVs also participate in specialized protein sorting pathways in specific tissues, influencing physiological ..
  19. FASEB SRC on Mitochondrial Assembly &Dynamics in Health, Disease &Aging
    Antoni Barrientos; Fiscal Year: 2011
    ..of respiratory chain components, transcriptional and translational regulatory circuits controlling mitochondrial biogenesis and function, mitochondrial DNA maintenance, mitochondrial-nuclear and mitochondrial-cytoplasm cross talk, ..
    ANIL KUMAR JAISWAL; Fiscal Year: 2013
    ..of bladders from wild type and NQO1-/- mice revealed that PPAR?C1a /MUP1/MUP2 factors regulating mitochondrial biogenesis, energy metabolism and adhesion were down regulated in NQO1-/- mice...
  21. Novel PPAR-Independent Peroxisome Proliferators for Type 2 Diabetes
    LIDIA NIEROBISZ; Fiscal Year: 2011
    ..Compounds that increase peroxisome biogenesis in rodents universally improve metabolic syndrome and diabetic symptoms and result in decreased elevated plasma ..
  22. Characterization of the ER associated Biogenesis and Degradation of ENaC
    Teresa M Buck; Fiscal Year: 2013
    ..The goal of this proposal is to identify and characterize additional effectors of ENaC degradation and biogenesis using two model systems...
  23. Ribosomal Protein Gene Silencing in Neural Crest Stem Cells
    JOHNSON MING YU LIU; Fiscal Year: 2010
    Diamond Blackfan anemia (DBA) is one of a class of disorders linked to defects in ribosome biogenesis. While classified as a bone marrow failure syndrome, some DBA patients exhibit craniofacial anomalies...
  24. Phospholipid flip-flop in biogenic membranes
    Anant K Menon; Fiscal Year: 2013
    ..Lipid flip-flop across the ER is also required for membrane biogenesis: phospholipids that are synthesized on the cytoplasmic face of the ER must be translocated to the opposite face ..
  25. Sympathetic Regulation of AMPK in the control of non-shivering thermogenesis
    Jacob D Mulligan; Fiscal Year: 2012
    ..weight gain increases caloric expenditure of BAT via an AMPK-mediated increase in brown adipocyte mitochondrial biogenesis. This will be tested in wildtype and 11 AMPK knockout mice in vivo, as well as in vitro using pharmacological ..
  26. Skeletal Muscle Mitochondrial Adaptations to a Two-Year Caloric Restiction
    STEVEN RICHARD SMITH; Fiscal Year: 2012 of CR (phase 1 of this U01), we propose the hypotheses that 2 years of 25% CR will increase mitochondrial biogenesis and increase coupling in vivo;that these changes will be associated with a reduction in ex vivo mitochondrial ..
  27. Molecular Genetics of Caloric Restriction in Aging Flies
    Blanka Rogina; Fiscal Year: 2013
    ..elucidate the role mitochondria play in CR life span extension by assessing the mitochondrial physiology and biogenesis in the Indy/rpd3/Sir2 longevity pathway...
  28. Mitochondrial Biomarkers of Cardiomyopathy and Cure in Chagas Disease
    Nisha Jain Garg; Fiscal Year: 2013
    ..of genes/proteins involved in maintaining the oxidative phosphorylation (OXPHOS) pathway and mitochondrial biogenesis. In this proposal, we will develop tools to capture the mitochondrial alterations as an indicator of cardiac ..
  29. Redox Regulation of Lung Mitochondrial Biogenesis in Sepsis/Pneumonia
    Claude A Piantadosi; Fiscal Year: 2013
    ..provided by applicant): This is a revised application to study the regulation and the role of mitochondrial biogenesis and mitophagy in experimental sepsis and acute lung injury (ALI) caused by S. aureus...
  30. CFTR Biogenesis and Function in Epithelia
    Zsuzsa Bebok; Fiscal Year: 2012
    ..Our previous project concentrated on the biogenesis of the wild type (WT) and the most common disease causing mutant, ?F508 CFTR...
  31. Noncoding RNA targets of the spinal muscular atrophy protein
    Livio Pellizzoni; Fiscal Year: 2010
    ..Defects in the biogenesis and function of these novel ncRNA targets of SMN may represent the disease trigger of SMA...
    Pei Zhou; Fiscal Year: 2012
    ..development, these studies will provide fundamental insights into lipid A biology, chemistry and outer membrane biogenesis. PUBLIC HEALTH RELEVANCE: Half of all human bacterial pathogens are classified as "Gram-negative";they appear as ..
  33. Experimental Molecular Vaccines for Schistosomiasis
    Afzal A Siddiqui; Fiscal Year: 2012
    ..identified a novel schistosome protein that was originally identified to be involved in the surface membrane biogenesis. This phenomenon has been considered to be one of the mechanisms utilized by blood-dwelling worms to evade the ..
  34. Disease-related defects in dendritic cell processing of bacterial antigens
    Michael S Marks; Fiscal Year: 2012
    ..monocytogenes) in vivo. 2. To test whether LRO biogenesis and/or phagosome maturation in bone marrow-derived DCs (BMDCs) is altered in HPS mouse models lacking BLOC-1 or ..
  35. Mitoenergetic Failure in Brain Aging
    Gregory J Brewer; Fiscal Year: 2013 the peroxisome proliferator family, especially PGC1a, PPAR? and NRF, also known to stimulate mitochondrial biogenesis. Since changes in mitochondrial function with age persist in culture, in Aim 3, we will test the hypothesis that ..
    Joseph Avruch; Fiscal Year: 2013
    ..First identified in yeast, there TOR regulates transcription, ribosomal biogenesis, mRNA translation and protein turnover in response to nutrient availability...
  37. Mechanisms and Functions of Bimodally Targeted Cytochrome P450s to Mitochondria
    Narayan G Avadhani; Fiscal Year: 2012
    ..cytosolic endoprotease in modulating mt- targeting of proteins such as GR, RxR1, ER2, and other proteins on mt-biogenesis, and mt- function...
  38. Contractile Regulation of Cardiocyte Protein Synthesis
    Paul J McDermott; Fiscal Year: 2013
    ..receptor superfamily that activates transcription of a wide array of target genes required for mitochondrial biogenesis, fatty acid metabolism and oxidative phosphorylation...
  39. Role of dyskerin in oral epithelial homeostasis
    Faizan Alawi; Fiscal Year: 2013
    ..However, the mechanisms remain to be elucidated. Dyskerin is required for the biogenesis of ribonucleoproteins that incorporate small non-coding RNA molecules characterized by the H/ACA secondary ..
  40. Biogenesis of lipoarabinomannan in mycobacteria
    Mary Jackson; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Biogenesis of lipoarabinomannan in mycobacteria Summary Phosphatidylinositol mannosides (PIM) and their multiglycosylated counterparts, lipomannan (LM) and lipoarabinomannan (LAM) are complex ..
  41. Agnoprotein in JC virus virion biogenesis and replication
    Mahmut Safak; Fiscal Year: 2012
    ..of agnoprotein and led us to hypothesize that agnoprotein plays critical regulatory roles in JCV virion biogenesis and therefore in the progression of PML...
  42. 2011 Nucleic Acids Gordon Research Conference
    Christopher D Lima; Fiscal Year: 2011
    ..and Chromatin Dynamics;Co- and Post-transcriptional Regulation;Non-coding RNA;RNA Stability and Export;Ribosome Biogenesis, Function and Regulation;Nucleic Acid Structure and Catalysis...
    Guojun Bu; Fiscal Year: 2013
    ..However, the mechanisms that govern their biogenesis and transport to neurons are poorly understood...
  44. Rev-ERBa Regulates Mitochondrial Biogenesis, Adiposity, and Insulin Action
    Thomas H Reynolds; Fiscal Year: 2010
    ..proliferator-activated receptor-gamma (PPAR?) coactivator 1 alpha (PGC1a), a master regulator of mitochondrial biogenesis. Our preliminary data demonstrates that PGC1a is increased in mice treated with MnTBA porphyrin...
  45. AMP-activated protein kinase in the ischemic heart
    Lawrence H Young; Fiscal Year: 2012
    ..AMPK modulates major metabolic pathways, gene transcription, and mitochondrial biogenesis. Our laboratory has elucidated the importance of AMPK in the heart, where it protects against myocardial injury ..
  46. Metabolic flux in a model of reduced oxidative capacity
    CHARLES ROBERT EVANS; Fiscal Year: 2013
    ..syndrome, including relative obesity, dyslipidemia as well as reduced oxidative capacity, mitochondrial biogenesis rates, and mitochondrial mass compared to the HCR line...
  47. microRNA analysis of neurons generated from patient-specific iPSCs
    Herbert M Lachman; Fiscal Year: 2013
    ..unequivocally identified, a promising candidate is DGCR8, which codes for a nuclear protein involved in miRNA biogenesis. DGCR8 combines with Drosha to form the so-called "microprocessor," which produces miRNAs from long primary ..
  48. Genome-wide Association Study: Fetal Hemoglobin Phenotypes in Sickle Cell Disease
    Betty Pace; Fiscal Year: 2009
    ..research shows that interval training (INT) produces greater changes in exercise capacity, mitochondrial biogenesis, skeletal muscle enzymatic markers associated with carbohydrate metabolism, aerobic metabolism and fat oxidation ..
  49. Mitochondrial Inborn Errors in Metabolism
    Douglas C Wallace; Fiscal Year: 2013
    ..LHON and of benzofibrate in repairing mitochondrial and RGC oxidative damage through induction of mitochondrial biogenesis. Finally, we will prepare an AAV-mCAT vector for intra-orbital injection to transduce the mCAT catalytic ..
  50. Transport ATPases: From Molecules to Maladies
    Rajini Rao; Fiscal Year: 2010
    ..and Mechanism of ATPases, Membrane Dynamics and Vesicle Fusion, Orphan ATPases, Transport ATPases and Cancer, Biogenesis, turnover and trafficking of ATPases...
  51. Mechanisms Involved in Age-Related Loss of Muscle Mass and Growth Response
    SUE CAROL BODINE; Fiscal Year: 2012 the activation of protein degradation and a concomitant inhibition of translation initiation and ribosome biogenesis resulting in negative protein balance...
  52. Dissecting the Role of Unfolded Protein Response in Alcoholic Liver Disease
    Ling Qi; Fiscal Year: 2013
    ..This is a very attractive model because the ER is the predominant site for lipid biosynthesis, lipid droplet biogenesis, and alcohol detoxification, and because hepatic ER has been reported to expand in alcoholics...
  53. A Protein Biologic for the Treatment of Sepsis
    Rafal M Smigrodzki; Fiscal Year: 2010
    ..Experimental manipulations, such as overexpression of transcription factors that enhance mitochondrial biogenesis (e.g...
  54. Biochemistry and Cell Biology of CHC22 Clathrin
    Frances M Brodsky; Fiscal Year: 2013
    ..sort cargo during receptor-mediated endocytosis from the plasma membrane (PM), endosomal traffic, and organelle biogenesis from the trans-Golgi network (TGN)...
  55. Function of the Zinc Finger Protein ZPR1 in neurodegeneration
    LAXMAN DASS GANGWANI; Fiscal Year: 2013
    ..effect of ZPR1 deficiency on the stability and integrity of SMN complex, nuclear accumulation of SMN and snRNP biogenesis. The findings of proposed study will provide insights into the function of ZPR1 in the nuclear accumulation of ..
  56. Functional Genomic Dissection of Refractory Anemia
    BENJAMIN LEVINE EBERT; Fiscal Year: 2013
    ..Alternatively, or additionally, abnormal ribosome biogenesis could lead to dysregulated mRNA translation and altered production of specific proteins...
  57. PPARgamma and PPARgamma agonists in septic shock
    Basilia Zingarelli; Fiscal Year: 2013
    ..exhibits reduced nuclear expression of the PPAR? coactivator 1-? (PGC-1?), the master regulator of mitochondrial biogenesis, and reduced activation of AMP-activated protein kinase (AMPK), a crucial energy status sensor, which is known ..
  58. High-Content Screening for Peroxisome Biogenesis for Type-II Diabetes.
    JONATHAN ZACHARY SEXTON; Fiscal Year: 2013
    ..provided by applicant): We will optimize a high-content assay to identify compounds that promote peroxisome biogenesis in human cells...
  59. Model systems for hematologic disorders caused by ribosomal protein deficiency
    George Thomas; Fiscal Year: 2010
    ..Although it is known that deficiencies in ribosomal protein expression result in disrupted ribosome biogenesis, the molecular mechanisms by which such defects lead to marrow failure and MDS are unclear...
  60. Cthrc1 function as a novel TGF-beta antagonist in the vasculature
    Volkhard Lindner; Fiscal Year: 2013
    ..Cthrc1 has an unexpected biogenesis because as it contains a signal peptide for secretion yet remains in the cytoplasm of differentiated smooth ..
  61. Effects of Dietary Fat on Contractile and Mitochondrial Function in Heart Failure
    Margaret P Chandler; Fiscal Year: 2010
    ..operate to stimulate cardiac gene expression of the FA metabolic pathways and regulate OX PHOS and mitochondrial biogenesis respectively...
  62. Genetics and Genomics of the yidC paralogs in Streptococcus mutans
    SARA MARIE RASER PALMER; Fiscal Year: 2013
    ..mutans to cause disease are located in the cytoplasmic membrane or are secreted outside of the cell. Proper biogenesis of the cell envelope of S...
  63. High-Throughput Assay for the Discovery of Small Molecule Inhibitors of microRNA
    Alexander Deiters; Fiscal Year: 2011
    ..Although the connections between those miRNAs and human diseases have been made, very little is known about the biogenesis and regulation of individual microRNAs in healthy tissue, and what causes their misregulation in diseased tissue...
  64. Host proteins in Vaccinia viral membrane biogenesis
    Victor Hsu; Fiscal Year: 2004
    ..Thus, we propose to elucidate how the COPI coat complex might play a role in viral biogenesis. First, we will determine the stage of viral biogenesis when COPI is first detected on viral membranes...
  65. Mitochondrial Assembly and Dynamics in Health and Disease
    Carla Koehler; Fiscal Year: 2009
    ..of respiratory chain components, transcriptional and translational regulatory circuits controlling mitochondrial biogenesis and function, mitochondrial-nuclear and mitochondrial cytoplasm cross talk, mitochondrial protein sorting, ..
  66. Analysis of Polyhydroxyalkanoate Inclusion Biogenesis
    Douglas Dennis; Fiscal Year: 2009
    ..The general goal of this research is to elucidate the mechanism of polyhydroxyalkanoate inclusion biogenesis. Polyhydroxyalkanoates (PHAs) are bacterial polymers that are synthesized in inclusions when carbon levels are ..
  67. Roles of Streptococcus mutans YidC1 and YidC2 in membrane biogenesis
    SARA RASER PALMER; Fiscal Year: 2010 every eukaryotic, archaeal and prokaryotic cell implying the existence of a back-up mechanism for membrane biogenesis and stress tolerance in this organism...
  68. Role of BLOC-3 in Lysosome and Melanosome Biogenesis
    ESTEBAN DELL ANGELICA; Fiscal Year: 2007
    ..Abnormalities in melanosome biogenesis seem to underlie the pigmentation defects and visual problems associated with several human genetic disorders, ..
  69. Cell wall biogenesis in Cryptococcus neoformans
    Deborah Fox; Fiscal Year: 2004
    ..neoformans is a direct result of fundamental differences in the way cell wall biogenesis is regulated in this organism compared to other fungi and that the identification of additional components of ..
  70. 2012 Iron Sulfur Enzymes Gordon Research Conference
    Markus W Ribbe; Fiscal Year: 2012
    ..of bringing together the world's leading experts in iron-sulfur clusters, focusing on enzymatic mechanisms, biogenesis, roles in regulation and in human disease in a uniquely cross-disciplinary manner...
  71. Adipose Tissue-specific Angiogenesis and Insulin Sensitivity
    Silvia Corvera; Fiscal Year: 2009
    ..paradoxical finding that thiazolidinediones such as rosiglitazone, that cause net weight gain by stimulating the biogenesis of new adipose cells, ameliorate insulin resistance...
  72. Oligodendrocyte Differentiation and Myelin Biogenesis
    Rashmi Bansal; Fiscal Year: 2007
    ..this project is to develop a detailed understanding of the molecular mechanisms of OL differentiation and myelin biogenesis in order to contribute to an informed intervention in clinical settings that will encourage more substantial ..
    David Pennock; Fiscal Year: 1990
    The long term goals of this project are to understand the molecular mechanisms of cilia biogenesis. Towards that end two Tetrahymena thermophila mutants with temperature-sensitive defects in both cilia biogenesis and some aspect of the ..
  74. Retrograde Signaling in Alcohol-Induced Mitochondrial Stress and Biogenesis.
    Neil Kaplowitz; Fiscal Year: 2009
    ..g., Hsp60 and 10) and mitochondrial biogenesis in response to stress to mitochondria (e.g...
  75. Assembly of the AI5-beta Group I Intron RNP
    Edward Turk; Fiscal Year: 2007
    ..DESCRIPTION (provided by applicant): This proposal will elucidate the mechanisms of ribonucleoprotein (RNP) biogenesis. RNPs play a central role in nearly all aspects of RNA biology, including transcription, RNA localization, ..
  76. Novel peroxisomal genes in the yeast Pichia pastoris
    James Cregg; Fiscal Year: 2005
    ..In humans, peroxisomes are essential for the metabolism of certain lipids, and defects in their biogenesis are responsible for a family of lethal genetic disorders termed peroxisome biogenesis disorders (PBDs)...
    Joseph Bass; Fiscal Year: 2003
    ..Insulin resistance is a predisposing factor for diabetes and can be induced by impaired biogenesis of the insulin receptor, a transmembrane protein tyrosine kinase...
  78. Mutational Analysis of Peroxisome Biogenesis Disorders
    Joseph Hacia; Fiscal Year: 2006
    unreadable] DESCRIPTION (provided by applicant): Peroxisome biogenesis disorders (PBDs) are inborn errors of metabolism with considerable clinical, biochemical, and genetic heterogeneity...
    Reuben Ramphal; Fiscal Year: 2007
    ..The long-term goal of this proposal is therefore to understand the biogenesis of this organelle in nature and disease...
  80. Ribosome Biogenesis in Diamond Blackfan Anemia
    Monica Bessler; Fiscal Year: 2007
    ..underlying mechanism responsible for the clinical manifestations in patients with DBA is a defect in ribosome biogenesis. We propose 1) that RPS19 mutations cause a defect in pre-ribosomal RNA processing and ribosome biogenesis that, ..
    PAUL LAZAROW; Fiscal Year: 2002
    The long term objectives of this research are to understand the biogenesis of peroxisomes in molecular detail and to identify and characterize human genes that cause fatal illness due to failure of peroxisome biogenesis...